CN102397172B - Extractive of sprouted soybean, and its preparation method and application - Google Patents
Extractive of sprouted soybean, and its preparation method and application Download PDFInfo
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- CN102397172B CN102397172B CN 201010275416 CN201010275416A CN102397172B CN 102397172 B CN102397172 B CN 102397172B CN 201010275416 CN201010275416 CN 201010275416 CN 201010275416 A CN201010275416 A CN 201010275416A CN 102397172 B CN102397172 B CN 102397172B
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Abstract
The invention discloses an extractive of sprouted soybean, and its preparation method and application. The extractive of sprouted soybean is prepared through the following method: crushed sprouted soybean is used as a raw material and extracted with an ethanol aqueous solution having a mass concentration of 75% so as to obtain the extractive of sprouted soybean; wherein, sprouted soybean is obtained through germination of soybean for 2 to 5 days. The method for extraction and separation provided in the invention is simple and convenient; an obtained product has a high purity and greatly improved quality. After soybean is sprouted, the content and quality of active components in soybean are improved. Sprouted soybean is used as a raw material and subjected to extraction; according to verification, the obtained extractive has powerful capability in removing free radicals and high capability in inhibiting the activity of tyrosinase. Therefore, the extractive of sprouted soybean can be used as a functional additive for delaying skin aging and has wide application in preparation of cosmetics, especially in preparation of cosmetics having the effect of delaying skin aging.
Description
Technical field
The present invention relates to a kind of extractive of sprouted soybean and preparation method thereof and application.
Background technology
The Semen sojae atricolor main active is soybean isoflavone, large soap saponin, soybean lecithin, soybean protein, aminoacid with multivitamin etc.Particularly soybean isoflavone also is plant estrogen.It is a kind of natural estrogen, and is without any side effects to human body.Be used for the minimizing that cosmetics can prevent skin collagen quantity, keep skin elasticity, the formation of prevention dermal melanin; Play clean white muscle skin, anti-lax, recover skin elasticity, solve the problem that produces of minimizing because of climacteric estrogen, create the skin the second spring.The earliest record about the bean sprout sees " Lingshu Jing, Miraculous Pivot " and Shennong's Herbal, and before the Song dynasty, the bean sprout only is medicinal, and it is edible it not to be used as vegetable.The lot of documents report, Semen sojae atricolor is after germinateing, its active component is further improved, change has occured in the constituent of soybean isoflavone, isoflavone content obviously improves, the content of aminoacid, various vitamin also significantly improves, and the variation of these active component has further been established it and has been used for the effect of cosmetics.
Summary of the invention
The purpose of this invention is to provide a kind of extractive of sprouted soybean and preparation method thereof.
Extractive of sprouted soybean provided by the present invention is to prepare according to the method that comprises the steps: the rudiment Semen sojae atricolor after to pulverize is as raw material, is that the ethanol water of 60%-80% extracts with mass concentration, obtains described extractive of sprouted soybean; Described rudiment Semen sojae atricolor obtained by soybean germination 2-5 days.
Wherein, described ethanol water preferred mass concentration is 75% ethanol water.
The proportioning of described rudiment Semen sojae atricolor and described ethanol water can be 1g: (10-20) ml is preferably 1g: 20ml, and the quality of described rudiment Semen sojae atricolor is with dry weight basis; Described extraction is specially supersound extraction; The temperature of described supersound extraction can be 40-70 ℃, is preferably 55 ℃; Time is 30-40 minute, is preferably 35 minutes; Supersonic frequency can be 60-100KHZ.The granularity of rudiment Semen sojae atricolor after the described pulverizing can be the 40-60 order.
Method of the present invention also comprises the step of following purification: after extracting end, centrifugal, collection extracting solution, ethanol evaporation in the described extracting solution is removed (to getting final product without the ethanol flavor), again surplus materials is applicable to organic solvent dissolution in the cosmetics with propylene glycol etc., collect solution, sterilization obtains the extractive of sprouted soybean behind the purification.
In purification step, described centrifugal rotating speed is 4000-5000rpm, is preferably 4000rpm; The centrifugal time is 10-30 minute, is preferably 20 minutes;
In purification step, the addition of described propylene glycol and the proportioning of described surplus materials are 10-15ml: 1g;
The temperature of described sterilization is 80-100 ℃, and the time is 30-45min.
The raw material rudiment Semen sojae atricolor of adopting among the present invention specifically obtains by the following method:
1) washing soybean is clean, soak;
2) Semen sojae atricolor after will soaking obtains described rudiment Semen sojae atricolor 20 ℃-25 ℃ lower sproutings 2-5 days.
Wherein, step 1) in, the mass ratio of described water and Semen sojae atricolor can be (1.5-3): 1, and preferred 2: 1; The time of described immersion can be 6-10h, preferred 8h, and soaking temperature is that room temperature gets final product;
Step 2) in, the concrete grammar of soybean germination is as follows: the Semen sojae atricolor after will soaking drains away the water and places and is lined with the cotton container of moistening dehydration, and covers with gauze, places to cultivate under the uniform temperature and sprouts, and Germination Temperature is 20 ℃～25 ℃, and preferred temperature is 22 ℃; Trickle once keeps Semen sojae atricolor moistening at regular intervals, and every 24h cleans once with clear water, prevents that the bean sprout is mouldy rotten; Germinating time is 2～5 days, preferred 3 days; After germination finishes the bean sprout is cleaned once with clear water, taken out 50 ℃ of oven drying at low temperatures.
A further object of the present invention provides the application of above-mentioned extractive of sprouted soybean.
The application of extractive of sprouted soybean provided by the present invention is its application in the preparation cosmetics; Especially preparation has the application in the function of delaying senility cosmetics.
The compositions that contains the cosmetics of extractive of sprouted soybean of the present invention and contain this extractive of sprouted soybean also belongs to protection scope of the present invention.
Extraction separation method provided by the present invention is easy, and products obtained therefrom purity is higher, and product quality has had very large improvement.Because contained isoflavone has the cellular metabolism of enlivening in the Semen sojae atricolor, prevent the minimizing of skin collagen quantity, keep skin elasticity, the formation of prevention dermal melanin, the effects such as the formation of obstruct speckle and the degeneration clouding colour of skin, can alleviate the skin problem that Women’s climacteric occurs comprehensively, allow the skin of imbalance reply equilibrium state.Saponin, multivitamin isoreactivity composition contained in the Semen sojae atricolor also have antioxidation, remove the functions such as free radical, prevention dermatitis, the infringement of opposing daylight, by the method for rudiment, have further improved the content and the quality of having improved active component of active component.Resulting extract has stronger radical scavenging activity and higher tyrosinase activity inhibition ability through verification experimental verification.Therefore extractive of sprouted soybean can be used as the functional additive of delay skin aging, in the preparation cosmetics, especially prepares the cosmetics with delay skin aging effect, has extensive use.
Description of drawings
Fig. 1 is the production technological process of preparation extractive of sprouted soybean provided by the invention;
Fig. 2 is soybean isoflavone and the bar diagram relatively of soybean saponin content before and after the Semen sojae atricolor rudiment;
Fig. 3 is the bar diagram relatively of nicotinic acid before and after the Semen sojae atricolor rudiment, vitamin B6, free biotin and Vitamin C content;
Fig. 4 is that the extractive of sprouted soybean scavenging ability of DPPH free radical is measured;
Fig. 5 is the mensuration of extractive of sprouted soybean restraint of tyrosinase activity.
The specific embodiment
Below by specific embodiment method of the present invention is described, but the present invention does not limit to therewith.Experimental technique described in the following embodiment if no special instructions, is conventional method; Described reagent and material if no special instructions, all can obtain from commercial channels.
Embodiment 1, preparation extractive of sprouted soybean
The production technological process of extractive of sprouted soybean as shown in Figure 1, concrete preparation process is as follows:
1) preparation rudiment Semen sojae atricolor
Get the Semen sojae atricolor of certain mass, clean up, add the deionized water of 2 times of Semen sojae atricolor quality, soak 8h under the room temperature; After soak finishing, Semen sojae atricolor drained away the water and place is lined with the cotton container of moistening dehydration, and covers with gauze, places 22 ℃ of lower cultivations to sprout; Trickle once keeps Semen sojae atricolor moistening at regular intervals, and every 24h cleans once with clear water, prevents that the bean sprout is mouldy rotten; Germinate and after 3 days the bean sprout is cleaned once with clear water, take out.
2) extract
50g bean sprout (with dry weight basis) is crushed to the 40-60 order, adds extracting solution (the 1000mL mass concentration is 75% ethanol water) by solid-liquid ratio 1g: 20ml; Under 55 ℃ with the supersonic frequency supersound extraction 35min of 100KHZ (add a cover preservative film, reduce evaporation); After naturally cooling to room temperature, under the rotating speed of 4000r/min centrifugal 20 minutes, get supernatant; Supernatant is carried out vacuum filter cake sucking filtration, collect filtrate; Rotary evaporation is removed the ethanol in the filtrate, by quality: volume (m: v)=1g: 15ml adds propylene glycol, makes to revolve and steams the afterwards abundant stirring and dissolving of remaining material, and remove by filter insoluble matter and get settled solution.Settled solution behind 90 ℃ of high temperature sterilize 30min, is obtained extractive of sprouted soybean solution.
The production technological process of extractive of sprouted soybean as shown in Figure 1, concrete preparation process is as follows:
1) preparation rudiment Semen sojae atricolor
Get the Semen sojae atricolor of certain mass, clean up, add the deionized water of 1.5 times of Semen sojae atricolor quality, soak 8h under the room temperature; After soak finishing, Semen sojae atricolor drained away the water and place is lined with the cotton container of moistening dehydration, and covers with gauze, places 22 ℃ of lower cultivations to sprout; Trickle once keeps Semen sojae atricolor moistening at regular intervals, and every 24h cleans once with clear water, prevents that the bean sprout is mouldy rotten; Germinate and after 2 days the bean sprout is cleaned once with clear water, take out.
2) extract
50g bean sprout (with dry weight basis) is crushed to the 40-60 order, adds extracting solution (the 1000mL mass concentration is 60% ethanol water) by solid-liquid ratio 1g: 10ml; Under 40 ℃ with the supersonic frequency supersound extraction 30min of 60KHZ (add a cover preservative film, reduce evaporation); After naturally cooling to room temperature, under the rotating speed of 4000r/min centrifugal 20 minutes, get supernatant; Supernatant is carried out vacuum filter cake sucking filtration, collect filtrate; Rotary evaporation is removed the ethanol in the filtrate, by quality: volume (m: v)=1g: 15ml adds propylene glycol, makes to revolve and steams the afterwards abundant stirring and dissolving of remaining material, and remove by filter insoluble matter and get settled solution.Settled solution behind 90 ℃ of high temperature sterilize 30min, is obtained extractive of sprouted soybean solution.
Embodiment 3, preparation extractive of sprouted soybean
The production technological process of extractive of sprouted soybean as shown in Figure 1, concrete preparation process is as follows:
1) preparation rudiment Semen sojae atricolor
Get the Semen sojae atricolor of certain mass, clean up, add the deionized water of 3 times of Semen sojae atricolor quality, soak 8h under the room temperature; After soak finishing, Semen sojae atricolor drained away the water and place is lined with the cotton container of moistening dehydration, and covers with gauze, places 25 ℃ of lower cultivations to sprout; Trickle once keeps Semen sojae atricolor moistening at regular intervals, and every 24h cleans once with clear water, prevents that the bean sprout is mouldy rotten; Germinate and after 5 days the bean sprout is cleaned once with clear water, take out.
2) extract
50g bean sprout (with dry weight basis) is crushed to the 40-60 order, adds extracting solution (the 1000mL mass concentration is 80% ethanol water) by solid-liquid ratio 1g: 20ml; Under 70 ℃ with the supersonic frequency supersound extraction 40min of 80KHZ (add a cover preservative film, reduce evaporation); After naturally cooling to room temperature, under the rotating speed of 4000r/min centrifugal 20 minutes, get supernatant; Supernatant is carried out vacuum filter cake sucking filtration, collect filtrate; Rotary evaporation is removed the ethanol in the filtrate, by quality: volume (m: v)=1g: 15ml adds propylene glycol, makes to revolve and steams the afterwards abundant stirring and dissolving of remaining material, and remove by filter insoluble matter and get settled solution.Settled solution behind 90 ℃ of high temperature sterilize 30min, is obtained extractive of sprouted soybean solution.
The functional examination test of embodiment 4, extractive of sprouted soybean
The extractive of sprouted soybean for preparing among the embodiment 1,2,3 is carried out respectively following experiment successively.
Used extractive of sprouted soybean solution in the following test, be that the extractive of sprouted soybean solution that embodiment 1,2,3 prepares is designated as mass concentration is 100% extract solution, the extract solution of other concentration carries out corresponding dilution with this solution and obtains.
1, the comparison of active component component content before and after the soybean germination
The rudiment Semen sojae atricolor that embodiment 1 is prepared gets dry rudiment Semen sojae atricolor in 50 ℃ of oven drying at low temperatures.Measure the content of active component in dried bean and the dry rudiment Semen sojae atricolor.
The mensuration of soybean isoflavone is carried out with reference to the method that provides such as Publication about Document: 1) Ju Xingrong, and Yuan builds, Wang Haifeng, the research of three-wavelength Determination of soybean isoflavone by UV spectrophotometry, Food Science, the 22nd phase of calendar year 2001: 46-48; 2) Shen Haijin, Wang Haifeng, Yuan Jian etc., the research that soybean germination phase isoflavone content changes and the impact of promoter thereof, Chinese grain and oil journal, the 6th phase: 75-78 in 2006.
The mensuration of soybean saponin content is carried out with reference to the method that provides such as Publication about Document: Ruan Hongsheng, Chen Zhibao, Zhu Dan etc., the research of soybean saponin extraction process in the bean cake, food science and technology, the 3rd phase of 15 volumes: 15-17 in 2008.
The mensuration of Vitamin C content is carried out with reference to the method that provides such as Publication about Document in the bean sprout: Guo Hongzhuan, Lu Zhanguo, Wang Caiyan, ascorbic Fluctuation research in the bean sprout growth process, food research and development, the 27th volume second phase in 2006.
The mensuration of the mensuration of nicotinic acid, the mensuration of vitamin B6, free biotin adopts respectively the method for GB/T 5009.89-2003, GB/T 5009.154-2003, GB/T 5413.19-197 to carry out.
The experimental result of the rudiment Semen sojae atricolor that embodiment 1 obtains is seen Fig. 2 and Fig. 3.As seen from the figure, compare with germinated soybean not, the content of soybean germination soybean isoflavone, vitamin C, nicotinic acid, free biotin after 3 days is improved significantly, and especially vitamin C does not exist dried Semen sojae atricolor, and the content of soybean saponin and vitamin B6 does not have significant change before and after soybean germination.3 days Semen sojae atricolor of sprouting of the present invention is described, wherein contained active component content is higher.The result of the rudiment Semen sojae atricolor that obtains among the embodiment 2 and 3 and the above results are without significant difference.
2, scavenging ability of DPPH free radical is measured
DPPH clearance rate assay method carries out according to the method that provides such as Publication about Document: Zheng Dagui, and Ye Qing, Ye Hongde, remaining redundancy due to misprinting, the DPPH method is estimated the antioxygenic property of Vc, different Vc and derivant thereof, food industry science and technology, the 04th phase: 113-115 in 2008.
1,1-diphenyl-2-trinitrophenyl-hydrazine (DPPH) is a kind of stable organic free radical, be 517nm at the visible region maximum absorption band, in alcoholic solution, each DPPH molecule can generate a stable nitrogen free radical that contains in solution, have typical purple, when it with provide the free radical scavenger of 1 electronics to do the time spent, generate colourless product, the typical purple of solution is shoaled.Its fading extent becomes stoichiometric relationship with the sharing electron number.Thereby available spectrophotography carries out quantitative analysis, detects the free radical scavenging situation, thus the oxidation resistance of assess sample.Its ability represents that with clearance rate clearance rate is larger, shows that then its oxidation resistance is stronger.
Experimental technique: the extracting solution of getting certain mass carries out gradient dilution with propylene glycol, and extract concentration is respectively 0g/100ml, 1g/100ml, 2g/100ml, 3g/100ml, 4g/100ml, 5g/100ml, 6g/100ml after the dilution.Get isopyknic liquid to be measured and 2 * 10
-4The DPPH solution of mol/L adds mixing in the same test tube (respectively getting 4ml), makes reference with 95% ethanol behind the 30min, surveys its absorbance A under 517nm
3Measure simultaneously 2 * 10
-4The DPPH solution of mol/L and the mixed absorbance A of equal-volume 95% ethanol
1And the absorbance A of measuring liquid to be measured and equal-volume 95% alcohol mixeding liquid
2
DPPH clearance rate=[1-(A
3-A
2)/A
1] * 100%
(A
3-A
2) eliminated the error that feed liquid self color causes, itself and A
1Ratio be exactly the shade degree that the free radical that is not eliminated displays, deduct this ratio with 1 and namely obtain feed liquid to the clearance rate of DPPH.
Used comparison-the germinated soybean extract is not prepared according to the extracting method among the embodiment 1, only needs the rudiment Semen sojae atricolor is replaced with not germinated soybean.
The experimental result of the extractive of sprouted soybean that embodiment 1 prepares is seen Fig. 4.As seen from the figure, free radical scavenging activity raises with the increasing of extract concentration, when extract concentration very high to the clearance rate of free radical during at 4g/100ml, than the free radical scavenging successful of germinated soybean extract not.Illustrate that extractive of sprouted soybean provided by the invention has stronger radical scavenging activity, has certain senile-resistant efficacy.The result of the extractive of sprouted soybean that embodiment 2 and 3 prepares and the above results are without significant difference.
3, the mensuration of restraint of tyrosinase activity
The melanic tyrosine that is formed in the dermal melanin cell tissue progressively is converted into eumelanin through intermediate such as DOPA, DOPA quinone, dopachrome, dihydroxy indoles under the effect of the enzymes such as tryrosinase; And then, the melanocyte tissue with melanin transfer in angle albuminous cell, and along with the horny layer cycle renews and comes off, the difference of human body complexion depends primarily on content and the distribution of eumelanin and pheomelanin, but when eumelanin is overrun growth and skewness, will cause local skin to cross black or pigmentation.Participate in that melanin is synthetic to mainly contain three kinds of enzymes: tryrosinase, dopachrome interconvertible enzyme (Dopachrom tautomerase) and dihydroxy indole carboxylic acid (DHICA) oxidase.Tryrosinase belongs to oxidoreductase, it is the synthetic main rate-limiting enzyme of melanin, its active size determines the quantity that melanin forms, many whitenings, the speckle dispelling product sold in the market all are to reach whitening function with the restraint of tyrosinase activity, so be to estimate the leading indicator of whitening cosmetic to the power of tyrosinase inhibitory action.
Catalytic reaction can occur in TYR enzyme and its substrate TYR.After in experimental system, having added the reagent that the effect of TYR inhibition of enzyme activity is arranged, can produce inhibitory action to catalytic reaction, add the reagent front and back in the absorption photometric at 475nm place by measuring, estimate reagent to the suppression ratio of TYR enzymatic activity.
Experimental technique:
(1) C
2After pipe prepared and shakes up, heating in water bath 10min in 37 ℃ of water-baths returned to zero under the wavelength 475nm.
(2) C
1Pipe solution prepares and shakes up, and behind 37 ℃ of water-bath 10min, adds tryrosinase 1ml, continues water-bath 10 minutes, measures C
1Absorbance.
(3) by (1) (2) same method, with T
2T is measured in zeroing
1Absorbance.
(4) calculation sample is to the maximum inhibition T (%) of tryrosinase.
Formula: T (%)=(C
1-T
1)/C
1* 100%
Table 1 reagent proportioning table
| Unit (mL) | C 1 | C 2 | T 1 | T 2 |
| TYR | 2 | 2 | 2 | 2 |
| |
0 | 0 | 2 | 2 |
| PBS | 4 | 5 | 2 | 3 |
| Tryrosinase | 1 | 0 | 1 | 0 |
| Cumulative volume | 7 | 7 | 7 | 7 |
Annotate: C
1And T
1All add the 1mL tryrosinase, enzyme is lived and is 100U/mL.
The experimental result of the extractive of sprouted soybean that embodiment 1 obtains is seen Fig. 5.As seen from the figure, the rudiment soybean extract strengthens with the rising that plays concentration the inhibition of tyrosinase activity, and suppression ratio reaches more than 60% when extract concentration is 6g/100ml, and inhibition is obvious.Illustrate that extractive of sprouted soybean provided by the invention has higher tyrosinase activity and suppresses ability, can be used for preparing cosmetics, especially be used for preparing the cosmetics with slow down aging and white-skinned face function.The result of the extractive of sprouted soybean that embodiment 2 and 3 obtains and the above results are without significant difference.
Claims (3)
1. adopt the application of extractive of sprouted soybean in the preparation cosmetics of following method preparation;
The preparation method of described extractive of sprouted soybean, rudiment Semen sojae atricolor after comprising the steps: to pulverize is raw material, be that the ethanol water of 60-80% extracts with mass concentration, after extracting end, centrifugal, collection extracting solution is removed the ethanol evaporation in the described extracting solution, again surplus materials is dissolved with propylene glycol, collect solution, sterilization obtains the extractive of sprouted soybean behind the purification;
The proportioning of described rudiment Semen sojae atricolor and described ethanol water is 1g: 10-20ml, and the quality of described rudiment Semen sojae atricolor is with dry weight basis; The described supersound extraction that is extracted as; The temperature of described supersound extraction is 40-70 ℃, and the time is 30-40 minute, and supersonic frequency is 60-100kHz; The granularity of rudiment Semen sojae atricolor after the described pulverizing is the 40-60 order;
Described rudiment Semen sojae atricolor obtains by the following method:
1) washing soybean is clean, soak;
2) Semen sojae atricolor after will soaking obtains described rudiment Semen sojae atricolor 20 ℃-25 ℃ lower sproutings 2-5 days;
Step 1) in, the mass ratio of described water and Semen sojae atricolor is (1.5-3): 1; The time of described immersion is 6-10h, and soaking temperature is room temperature; Step 2) in, the temperature of described sprouting is 22 ℃, and the time is 3 days;
Described centrifugal rotating speed is 4000-5000rpm; The centrifugal time is 10-30 minute;
The addition of described propylene glycol and the proportioning of described surplus materials are 10-15ml: 1g;
The temperature of described sterilization is 80-90 ℃, and the time is 30-45min.
2. application according to claim 1 is characterized in that: described cosmetics are the cosmetics with slow down aging and white-skinned face function.
3. cosmetics with slow down aging and white-skinned face function is characterized in that: described cosmetics contain the extractive of sprouted soybean that adopts following method preparation;
The preparation method of described extractive of sprouted soybean, rudiment Semen sojae atricolor after comprising the steps: to pulverize is raw material, be that the ethanol water of 60-80% extracts with mass concentration, after extracting end, centrifugal, collection extracting solution is removed the ethanol evaporation in the described extracting solution, again surplus materials is dissolved with propylene glycol, collect solution, sterilization obtains the extractive of sprouted soybean behind the purification;
The proportioning of described rudiment Semen sojae atricolor and described ethanol water is 1g: 10-20ml, and the quality of described rudiment Semen sojae atricolor is with dry weight basis; The described supersound extraction that is extracted as; The temperature of described supersound extraction is 40-70 ℃, and the time is 30-40 minute, and supersonic frequency is 60-100kHz; The granularity of rudiment Semen sojae atricolor after the described pulverizing is the 40-60 order;
Described rudiment Semen sojae atricolor obtains by the following method:
1) washing soybean is clean, soak;
2) Semen sojae atricolor after will soaking obtains described rudiment Semen sojae atricolor 20 ℃-25 ℃ lower sproutings 2-5 days;
Step 1) in, the mass ratio of described water and Semen sojae atricolor is (1.5-3): 1; The time of described immersion is 6-10h, and soaking temperature is room temperature; Step 2) in, the temperature of described sprouting is 22 ℃, and the time is 3 days;
Described centrifugal rotating speed is 4000-5000rpm; The centrifugal time is 10-30 minute;
The addition of described propylene glycol and the proportioning of described surplus materials are 10-15ml: 1g;
The temperature of described sterilization is 80-90 ℃, and the time is 30-45min.
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| KR102417301B1 (en) * | 2015-10-30 | 2022-07-06 | (주)아모레퍼시픽 | Composition for skin whitening containing soyasaponin |
| CN111317700A (en) * | 2020-04-02 | 2020-06-23 | 安赛搏(重庆)生物技术有限公司 | Method for preparing cosmetic base water by fermenting rice young bud raw materials |
| CN115607489A (en) * | 2022-10-09 | 2023-01-17 | 皓雨(广州)化妆品制造有限公司 | Cosmetic raw material containing germination secretion and preparation method of secretion |
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| 李笑梅，等.发芽大豆异黄酮提取工艺条件的研究.《食品工业科技》.2009,第30卷(第11期),第221页摘要，左栏倒数第2行-右栏第16行. * |
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