KR101805015B1 - Skin external composition containing Citron Extract, Ulmus Davidiana root Extract, and Salicornia herbacea Extract - Google Patents

Abstract

The present invention increases the gene expression of hyaluronic acid synthase 2 (HAS2) present in human cells by containing two or more kinds of mixed components selected from the group consisting of citron extract, roots extract and green tea extract as an active ingredient Promoting the synthesis of hyaluronic acid in the human body, promoting the synthesis of Versican, and inhibiting the production of MMP-12 responsible for the degradation of Versican, thereby strengthening the structure of the extracellular matrix and improving skin elasticity and skin moisturizing effect And an external preparation composition.

Description

[0001] The present invention relates to a skin external composition containing citrus extract, Ulmus davidiana root extract, and Salicornia herbacea Extract,

The present invention includes hyaluronic acid synthase 2 (HAS2) gene expression in human cells by containing citron extract, rhizome extract, and green tea extract as an active ingredient, thereby activating the hyaluronic acid producing action of the human body, The present invention relates to a composition for external application for skin, which promotes the synthesis of versican and inhibits the production of MMP-12 responsible for the degradation of versican, thereby strengthening the structure of the extracellular matrix, thereby improving skin elasticity and skin moisturizing effect.

Conventional studies on ULMUS DAVIDIANA, which is the skin of elm roots, were about immunosuppressants and edible beverages. In Korean Patent No. 10-0439939, the effect of improving wrinkles, promoting collagen synthesis And an anti-aging composition containing a polysaccharide extract isolated from low molecular weight and high molecular weight hyaluronic acid and rhizome of the present invention is also excellent in comparison with the present invention. The effect is insufficient.

Korean Patent Registration No. 10-0439939

Thus, the inventors of the present invention conducted an ELISA in real-time DNA chain polymerization reaction in a cell culture state to increase the expression of the hyaluronic acid synthase 2 (HAS2) gene in citron extract, rhizome extract, green tea extract, Which increases the synthesis of hyaluronic acid and increases the expression of a virucan gene that enhances the extracellular matrix structure by binding to hyaluronic acid and collagen bundles and induces a decrease in the MMP-12 gene that causes the degradation of versican And completed the present invention.

Accordingly, the present invention relates to a pharmaceutical composition containing as an active ingredient a citron extract, a radish extract, a radish extract, a green tea extract or a combination extract thereof, which promotes hyaluronic acid production and strengthens the structure of an extracellular matrix component, A composition for promoting the production, or a composition for skin moisturizing enhancement and anti-aging.

In order to achieve the above object, the present invention provides a composition for external application for skin moisturizing and skin aging, which comprises citron extract, roots extract, green tea extract or a complex component thereof as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The citron used as the active ingredient of the present invention is Citrus Junos ), and the citron is an evergreen tree of the mulberry tree with a diceyle plant. It is native to China and planted as a fruit tree in the south. It is about 4m high. There are pointed spines on branches. Leaves are alternate phyllotaxis, ovate long oval, dull serrate on edge, wings on petiole. The flowers are bisexual and bloom in May to June, and they run one on the axilla. There are 5 calyxes and petals, 20 of which are merged together or divided into five. Fruit is long-bellied, 4-7cm in diameter, ripened in September-October. Its color is bright yellow and its surface is rugged and fragrant. Reproduction is done by seeding or grafting. Yuzu, a fruit, is soft and juicy, but it is strong in sour taste and fragrant, so it is used for cooking. In addition, it is known that when you drink citron with sugar and drink tea, you can win the cold well. In the old days, to win the cold, I put the citron into the bath and put on the bath.

Root bark used as an active ingredient of the present invention is a name collectively referred to as root bark of elm. Elm is a deciduous broad-leaved arboreous tree, with a length of 20m and a diameter of 60cm. The tree bark is gray-brown and the branch has a short brown hairs on reddish brown. . Wood is used for building materials, machinery materials, ship materials, construction materials, firewood. The root bark of elm is used as a medicinal material in oriental medicine, called oriental or milky, and its taste is sweet and its quality is fair. Swelling, urine discomfort, constipation, coughing, coughing, solitary,

Salicornia herbacea is a herbal medicine used in the present invention. The herbaceous plant is a plant belonging to the genus Liliaceae. It grows to a height of 10 to 30 cm and is divided into branches. The stem is dark green or yellow at first, then it turns red when it is autumn, and it is like a small cactus. Leaves are degenerate and almost invisible, with small scale scales of membrane. From August to September, three flowers bloom at the upper node of branches. The fur is fleshy and plump and has 1-2 stamens and 2 styles. Fruits are fleas and are wrapped in horns and have black seeds. Seawater is a one-year-old herb that grows near the tidal flats and inland saline areas. It is an excellent treatment for various intractable diseases such as cancer, myoma, sinusitis, hypertension, hypotension, back pain, diabetes, bronchial asthma, hypothyroidism, hyperthyroidism, skin disease and arthritis. It is known to carry.

The citrus juice extract, rhizome extract and green tea extract used in the present invention can be prepared by a commercially available method or a commonly used method, and the production method thereof is not particularly limited, but is preferably prepared by the following method .

The extract may be prepared by hot water extraction, supercritical extraction, subcritical extraction or the like, preferably by ultra-high pressure extraction.

The citron extract, roots extract and green tea extract used in the present invention can be obtained by extracting each medicinal material with water or an organic solvent. The organic solvent used in the present invention may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, preferably 80% ethanol. Further, a mixed solvent of an organic solvent and water may be used, and preferably 1: 1.

The composition of the present invention preferably contains at least two kinds selected from the group consisting of citron extract, roots extract and green tea extract in an amount of 0.0001 to 20% by weight based on the total weight of the composition. If the content of the complex extract is less than 0.0001% by weight, it is impossible to obtain an increase in hyaluronic acid production, acceleration of the production of versatile and inhibition of MMP-12 production by the extract, and if it exceeds 20% by weight, Because it is inefficient.

The composition according to the present invention is more effective when two or more kinds of the extracts are mixed and used than when the extracts are used alone. Preferably, the citrus extract and the mixture of citrus peel and citrus peony extracts are mixed at a weight ratio of 1: 1 to 1: 1, and the mixture is used in an amount of 0.0001 to 20% by weight, but not particularly limited thereto. . In addition, when the mixture of citron extract and rhizome extract is mixed with a green tea extract, the effect is more excellent. Preferably, citron extract, rhizome extract and green tea extract are mixed at a weight ratio of 1: 1: 1, They may be mixed and used in an appropriate ratio within the formulation.

The composition for external application for skin of the present invention is not particularly limited in its formulation. For example, the external composition for skin of the present invention may have cosmetic formulations of softening agent, nutritional lotion, massage cream, nutritional cream, pack, gel or skin adhesive type, , Gel, cream, patch, or spray.

The composition of the present invention increases the expression of the HAS2 gene, thereby increasing the synthesis of hyaluronic acid. In addition, it increases the expression of the versatile gene, which not only promotes the synthesis of versican, but also reduces the expression of the MMP-12 gene, thereby inhibiting the production of MMP-12, which is responsible for the degradation of versatile. Accordingly, the present invention has the effect of promoting the production of hyaluronic acid and strengthening the structure of extracellular matrix components, thereby suppressing skin elasticity deterioration due to aging and promoting skin moisturization.

The composition for external application for skin of the present invention contains a combination of citron extract and rhizome extract, which are not a single component, to significantly increase gene expression of a hyaluronic acid synthase present in human cells, thereby enhancing hyaluronic acid production promoting activity And at the same time, it promotes the production of vermiculite which strengthens the extracellular matrix structure, and inhibits the production of MMP-12 responsible for the degradation of versatil. Therefore, it can be used for preventing dryness of skin and aging of skin .

FIG. 1 is a graph showing the expression of hyaluronic acid synthase at the mRNA level after treatment of citron extract, rhizome extract, and green tea extract with HaCaT cells.
FIG. 2 is a graph showing the expression of a versatile gene at the level of mRNA after treatment of Haeca T cells with citron extract, yuukee extract, and green tea extract.
FIG. 3 is a graph showing the expression of MMP-12 gene by UV at the mRNA level after treatment of HaCaT cells with citron extract, root extract, and green tea extract.
FIG. 4 is a graph showing the results of measuring the amount of hyaluronic acid by the ELISA method after treating Haeca T cells with citron extract, Rootgrass bark extract, and green tea extract.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples. However, the present invention is not limited by these examples.

[Preparation Example 1] Preparation of citron extract

10 g of citron were extracted with 90 L of purified water as a solvent for 3 hours while maintaining a low temperature of below 30 캜 at 1500 atm using an ultra-high pressure apparatus. Then, the alien substance was removed by centrifugation (9000 rpm, 15 ° C, 30 min), and then diluted 10 times with a diluting solvent such as purified water, butyleneglycol, phenoxyethanol or the like to produce citron extract.

[Production Example 2] Preparation of extracts of Root Root Extract

100 g of yugaekpyu was extracted with 1000 L of purified water for 3 hours while maintaining a low temperature of 30 캜 or less at 1500 atm using an ultra high pressure apparatus. Then, the foreign material was removed by centrifugation (9000 rpm, 15 ° C, 30 min), filtered with a filter cloth, and diluted 10 times with a diluting solvent such as purified water, butyleneglycol, phenoxyethanol or the like.

[Production Example 3] Preparation of Leeky Leaf Extract

100 g of green tea was extracted with 1000 L of purified water for 3 hours while maintaining a low temperature of 30 캜 or less at 1500 atm using an ultra high pressure apparatus. Then, the foreign material was removed by centrifugation (9000 rpm, 15 ° C, 30 min), filtered with a filter cloth, and diluted 10 times with a diluting solvent such as purified water, butyleneglycol, phenoxyethanol or the like.

 [Test Example 1] Cell line and cell culture

HaCaT cells (human keratinocyte cell line, cell line service), a human keratinocyte, were cultured in DMEM medium (Dulbecco's modified Eagle's Medium, Gibco 1210-0038) containing 10% fetal bovine serum, At 37 ° C in a 5% CO ₂ incubator for 18 hours.

[Test Example 2] Expression of HAS2 and Versican in HaCaT Cells by Treatment with Yuza, Yugenmei and Green Tea Extracts

In order to confirm the effect of promoting the synthesis of hyaluronic acid and versatile by the composition of the present invention, the extract of the present invention is treated to measure the expression pattern of the hyaluronic acid synthase hAS2 gene and the versatile gene in the cells by the amount of mRNA Respectively.

Step 1. Extract treatment of cell line

The cell line cultured in Test Example 1 was treated with trypsin to prepare a single cell suspension. The cell suspension was dispensed into a T75 flask at a density of 1 × 10 ⁶ cells and cultured in a 37 ° C., 5% CO ₂ incubator for 24 hours.

The cultured cells were cultured in dimethylsulfoxide (DMSO) -treated medium (Comparative Example 1), the extracts of Preparation Examples 1 to 3 were dissolved in dimethylsulfoxide and citron extract (Comparative Example 2) (Comparative Example 3) and green tea extract (Comparative Example 4) were each treated at 10 ppm each. The extracts of Preparation Examples 1 and 2 were dissolved in dimethylsulfoxide and 1: 1 of yuzu (Example 2) treated with 5 ppm of the combined extract (Example 1), yuza extract, rhizome extract and green tea extract 1: 1: 1 were mixed and cultured in a medium (Example 2) Lt; / RTI >

Step 2. Quantitative Reverse transcription PCR through HAS2 , Varsikan  Gene mRNA  Confirmation of increased synthesis

The cells were collected, washed with cold 4 ° C phosphate buffered saline (PBS), and 1 ml of TRIzol ™ reagent (Life Technologies, Inc.) was added to extract the total RNA to perform quantitative real-time DNA chain polymerization. Primer sequences of HAS2, versatile, and MMP-12 for real-time DNA chain polymerization reaction are shown in Table 1 below.

Name of the primer order HAS-2 forward primer 5'-TTTCTTTATGTGACTCATCTGTCTCACCGG-3 ' HAS-2 reverse primer
VersiChan forward primer
Versican Reverse primer
MMP-12 forward primer
MMP-12 reverse primer 5'-ATTGTTGGCTACCAGTTTATCCAAAGGG-3 '
5'-GTCACCTTCCAACTATCCGGT-3 '
5'-AGCACGGTAGTCCATTCTTTCT-3 '
5'-GGAGTCCCCGATCTCCATCAT-3 '
5'-ACGGTTCATGTCAGGTGTGTA-3 ' GAPDH forward primer 5'-CAACTACATGGTTTACATGTTCC-3 ' GAPDH reverse primer 5'-GGACTGTGGTCATGAGTCCT-3 '

The primers were based on SCI-grade papers.

1 μg of the extracted total RNA was added to ₂₅ μl of a reaction buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl ₂ , 0.1 M DTT, 10 mM dNTP and 40 units / μl RNase inhibitor, A primer of 0.5 μg / μl oligo (dT) ₁₆ and a reverse transcription polymerase of 200 units SuperScript II (SuperScript II, GiboBRL) were added and reacted at 42 ° C. for 1 hour. Then, 2.5 μl of the reverse reaction solution was added to AmpliTaq DNA polymerase [0.04 U; Perkin Elmer, Shelton, CT], 50 mM Tris (pH 8.3), 0.25 mg / ml Bovine Serum Albumin, 3 mM MgCl ₂ , 0.25 mM dNTPs, 1 / 50,000 dilution of SYBR Green I [Molecular Probes, Eugene, OR] PCR reaction buffer, 10 μM of primer was added, and 30 cycles of denaturation at 94 ° C. for 30 seconds, annealing at 53 ° C. for 30 seconds, and extension at 72 ° C. for 1 minute were carried out 30 times. Relative mRNA levels were analyzed by measuring SYBR Green I fluorescence changes using ICycler software. Quantitative expression levels of genes were corrected using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as an internal standard. The mRNA expression level of the HAS2 gene is shown in Fig. 1 and the mRNA expression level of the versatile gene is shown in Fig.

As shown in Figs. 1 and 2, when the extracts of yuzu, yugenmei and green tea were treated alone (Comparative Examples 2 to 4), the expression amounts of the HAS2 gene and the versatile gene were higher than those of Comparative Example 1 However, the expression level of the HAS2 gene and the vasicococcal gene was significantly increased in the experimental group (Example 1) using the combined prescription of the citron extract and the rhizome extract, compared with the single treatment group, The expression level of the HAS2 gene and the versatile gene was significantly increased in the experimental group (Example 2) using the combined formulation of the green tea extract and the small amount of the HAS2 gene and the versatile gene, The effect is shown.

Therefore, according to the present invention, the combined use of citron extract and rhizome extract can effectively increase the synthesis of hyaluronic acid, and when the complex extract further contains a green tea extract, the synthesis of hyaluronic acid can be remarkably increased, Thereby enhancing the extracellular matrix structure.

[Test Example 3] Reduction of expression of MMP-12 in HaCaT cells by treatment with yuza, yugenmei and green tea extract

In order to confirm the degradation effect of the degradation of versatile by the composition of the present invention, the expression pattern of the MMP-12 gene responsible for the degradation of vernig cell was treated by the amount of mRNA by treating the extract of the present invention.

Step 1. To the cell line UV  And extract treatment

HaCaT cells cultured in Test Example 1 were cultured in a 100 mm cell culture dish at a density of 1 × 10 ⁶ cells, irradiated with ultraviolet ray B at 10 mJ / cm 2 using an FS40 lamp (Westinghouse, Pittsburgh, Pa., USA) (Comparative Example 6) treated with dimethylsulfoxide (DMSO), the citron extract (Comparative Example 7) in the form of dissolving the extracts of Preparation Examples 1 to 3 in dimethylsulfoxide, (Comparative Example 8) and green tea extract (Comparative Example 9) were each treated at 10 ppm each. The extracts of Preparation Examples 1 and 2 were dissolved in dimethylsulfoxide, and the citron extract and the extracts were mixed to obtain a total extract of 5 ppm (Example 3), a citron extract, a yucca extract, a yuukee extract and a green tea extract were mixed and cultured in a medium (Example 4) in which a total of 5 ppm of the extract was treated (Example 4) for 24 hours in a 5% CO ₂ incubator . At this time, the control group was a sample not irradiated with UV (Comparative Example 5).

Step 2. Quantitative Reverse transcription PCR through MMP -12 gene mRNA  Confirm synthesis reduction

The cells were collected, washed with cold 4 ° C phosphate buffered saline (PBS), and 1 ml of TRIzol ™ reagent (Life Technologies, Inc.) was added to extract the total RNA to perform quantitative real-time DNA chain polymerization.

1 μg of the extracted total RNA was added to ₂₅ μl of a reaction buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl ₂ , 0.1 M DTT, 10 mM dNTP and 40 units / μl RNase inhibitor, A primer of 0.5 μg / μl oligo (dT) ₁₆ and a reverse transcription polymerase of 200 units SuperScript II (SuperScript II, GiboBRL) were added and reacted at 42 ° C. for 1 hour. Then, 2.5 μl of the reverse reaction solution was added to AmpliTaq DNA polymerase [0.04 U; Perkin Elmer, Shelton, CT], 50 mM Tris (pH 8.3), 0.25 mg / ml Bovine Serum Albumin, 3 mM MgCl ₂ , 0.25 mM dNTPs, 1 / 50,000 dilution of SYBR Green I [Molecular Probes, Eugene, OR] PCR reaction buffer, 10 μM of primer was added, and 30 cycles of denaturation at 94 ° C. for 30 seconds, annealing at 53 ° C. for 30 seconds, and extension at 72 ° C. for 1 minute were carried out 30 times. Relative mRNA levels were analyzed by measuring SYBR Green I fluorescence changes using ICycler software. Quantitative expression levels of genes were corrected using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as an internal standard. As a result, the mRNA expression level of the MMP-12 gene is shown in Fig.

As shown in Fig. 3, when the extracts of yuzu, yugenmei and green tea were treated alone (Comparative Examples 7 to 9), the expression level of MMP-12 gene was decreased to some extent as compared with Comparative Example 6, The expression level of the MMP-12 gene was significantly decreased in the experimental group (Example 3) using the combination of citron extract and guinea pig extract, compared to the single treatment group. In the case of the experimental group used (Example 4), the expression level of MMP-12 gene was remarkably decreased even though it was used in a smaller amount than that of the single treatment group, and it is also remarkable that the effect was remarkable compared with the two kinds of compound extracts.

Therefore, according to the present invention, when the citron extract and the rhizome extract are used in combination, the degradation of the extracellular matrix structure can be inhibited by decreasing the expression level of the MMP-12 gene. In addition, It is possible to remarkably reduce the degradation of virucan, thereby enhancing the extracellular matrix structure.

[Test Example 4] Increase of hyaluronic acid production in HaCaT cells by citron extract, Rootgrass extract and green tea extract

The cell line cultured in Test Example 1 was treated with trypsin to prepare a single cell suspension. The cell suspension was dispensed into a T75 flask at a density of 1 × 10 ⁶ cells and cultured in a 5% CO ₂ incubator at 37 ° C. for 24 hours.

The cultured cells were cultured in dimethylsulfoxide (DMSO) -treated medium (Comparative Example 10), the extracts of Preparation Examples 1 to 3 were dissolved in dimethylsulfoxide and citron extract (Comparative Example 11) (Comparative Example 12) and Green tea extract (Comparative Example 13) were each treated at 10 ppm each. The extracts of Preparation Examples 1 and 2 were dissolved in dimethylsulfoxide, and the citron extract and the extracts were mixed to obtain a total of 5 ppm Cultured in a medium (Example 6) treated with a total of 5 ppm of the extract (Example 5), Yuza extract, Rootgrass extract, and Hamcho extract, at 37 ° C in a total of 5 ppm, for 24 hours in a 5% CO ₂ incubator Respectively.

After 24 hours, the cell culture medium was recovered and centrifuged at 15,000 g for 5 minutes to remove the precipitated cells. The supernatant was collected and analyzed using an ELISA kit (Echelon Bioscience, Salt Lake, UT) The amount of hyaluronic acid was measured and the results are shown in Fig.

As shown in Fig. 4, the amount of hyaluronic acid was slightly increased compared to Comparative Example 10 in which yuzu, yugenmei and green tea extracts were treated alone (Comparative Examples 11 to 13) (Example 5), which was a combination of the extract of yuukee and green tea extracts, showed a significant increase in the amount of hyaluronic acid even though a small amount was used, In the case of Example 6), the amount of hyaluronic acid was remarkably increased even though it was used in a smaller amount than that of the single treatment group.

Therefore, according to the present invention, the combined use of citron extract and rhizome extract can effectively increase the synthesis of hyaluronic acid, and when the complex extract further contains a green tea extract, the synthesis of hyaluronic acid can be remarkably increased, This can reinforce the extracellular matrix structure and prevent skin elasticity loss and moisture content reduction due to reduction of hyaluronic acid.

 [Formulation Example 1] Nutritional lotion

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 2 below.

ingredient Content (% by weight) Citron extract 10 Acne extract 10 Green tea extract 10 Squalane 5.0 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

[Formulation Example 2] Nourishing cream

Nutrition creams were prepared according to the compositions shown in Table 3 below in a conventional manner.

ingredient Content (% by weight) Citron extract 10 Acne extract 10 Green tea extract 10 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 hardened castor oil 2.0 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

 [Formulation Example 3] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 4 below.

ingredient Content (% by weight) Citron extract 10 Acne extract 10 Green tea extract 10 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonyl phenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

[Formulation Example 4] ointment

Ointments were prepared in a conventional manner according to the compositions shown in Table 5 below.

ingredient Content (% by weight) Citron extract 10 Acne extract 10 Green tea extract 10 Purified water Balance glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Stearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Balance beeswax 4.1 Sum 100

Claims (10)

A cosmetic composition for moisturizing skin, comprising as an active ingredient, a mixture of citron extract, yuukee extract, and green tea extract. The cosmetic composition according to claim 1, wherein the effective ingredient is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. The cosmetic composition according to claim 1 or 2, wherein the composition promotes hyaluronic acid production. The cosmetic composition according to claim 1 or 2, wherein the composition increases expression of a hyaluronic acid synthase gene. 3. The cosmetic composition according to claim 1 or 2, wherein the composition increases the expression of a versatile gene. 3. The cosmetic composition according to claim 1 or 2, wherein the composition reduces the expression of the MMP-12 gene. The cosmetic composition according to claim 1, wherein the composition is a mixture of citron extract, rhizome extract, and green tea extract in a weight ratio of 1: 1: 1. A cosmetic composition for promoting skin elasticity comprising a mixture of citron extract, citron extract, yuukee extract and green tea extract as an active ingredient. The cosmetic composition according to claim 8, wherein the effective ingredient is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. [Claim 9] The cosmetic composition according to claim 8, wherein the composition comprises citron extract, rhizome extract and green tea extract at a weight ratio of 1: 1: 1.

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