CN102389145B - Method for preparing composite beverage by fermenting Cordyceps militaris - Google Patents
Method for preparing composite beverage by fermenting Cordyceps militaris Download PDFInfo
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 28
- 239000002131 composite material Substances 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title abstract 3
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 11
- 230000004151 fermentation Effects 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
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- 238000002386 leaching Methods 0.000 claims description 20
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- Non-Alcoholic Beverages (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing a composite beverage by fermenting Cordyceps militaris, which belongs to the nutrition and health beverage field. The composite beverage is prepared by using Cordyceps militaris, red date, Chinese wolfberry and honeysuckle as main raw materials, the method comprises the following steps: fermenting Cordyceps militaris to prepare fermentation liquor, respectively extracting red date, Chinese wolfberry and honeysuckle to obtain extract liquid, preparing the composite beverage according to the proportion that every 100mL beverage contains 40-60mL of Cordyceps militaris fermentation liquor, 10-20mL of Chinese wolfberry extract liquid, 2-8mL of honeysuckle extract and 25-40mL of red date extract liquid by adding water and seasoning auxiliary materials. The preparation method has the advantages of simple operation and short period. The prepared composite beverage by fermenting Cordyceps militaris has abundant nutrition and has auxiliary efficacies of raising human immunizing power, enhancing physical fitness, nourishing liver and improving eyesight, tonifying spleen and stomach, nourishing blood and tranquilizing mind, recuperating kidney and benefiting essence of life, aging resistance, antimutagenesis and antineoplastic.
Description
Technical field
The present invention relates to a kind of nutrient health-care beverage, specifically a kind of preparation method of composite beverage by fermenting Cordyceps militaris, belong to field of health care food.
Background technology
Cordyceps militaris extensively distributes in the world at China's northern Chinese caterpillar Fungus that is otherwise known as, and is a kind of famous and precious Chinese medicine and high tonic.Its active component is extremely similar to famous medicinal fungi Cordyceps sinensis with function, and some active component such as the content of material such as cordycepin, nucleosides are also higher than Cordyceps sinensis.Because natural Cordyceps militaris quantity is deficient, a large amount of excavations of people in addition constantly reduce the quantity of Cordyceps militaris, can not satisfy people's demand.Since last century the seventies; the researcher begins the liquid fermentation of Cordyceps militaris is studied both at home and abroad; studies show that; the Cordyceps militaris of liquid fermentation contains the multiple lifes such as cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, nucleosides, sterol: active substances; human body is had multiple significant physiological function, have strengthen immunity, regulate endocrine, the different physiological roles such as antifatigue, anti-oxidant, anti-ageing, antibiotic, antitumor, anti-anoxic, calmness, hypoglycemic, protection liver kidney and respiratory system.
Beverage market relates to tea, water, fruit juice, solid beverage four bulks at present.And the market of health drink is in low link, and the R﹠D and promotion of health drink kind has very large development space.Along with the raising of people's living standard and cultural quality, people will more pay attention to safety and sanitation, natural sex and the health of beverage when selecting beverage.Cordycepin beverage is several years ago appearing at beverage market, but natural health composition collocation Chinese caterpillar fungus drinks together such as discovery and red date, matrimony vine, honeysuckle not yet.
Summary of the invention
The invention provides a kind of preparation method of composite beverage by fermenting Cordyceps militaris, purpose is horn of plenty beverage market and provide a kind of nutritious, has the composite beverage by fermenting Cordyceps militaris of multiple physiological regulation function.
The present invention realizes with following technical scheme: a kind of preparation method of composite beverage by fermenting Cordyceps militaris, to make as primary raw material take Cordyceps militaris, red date, matrimony vine, honeysuckle, and concrete steps are as follows:
1) preparation of Cordyceps militaris zymotic fluid: the Pm14 cordyceps militaris link bacterial strain, cultivate 3-8d for 20-25 ℃ on the SDAY slant medium; Then in the SDY culture medium with 100-140r/min, 20-25 ℃, shaking flask is cultivated 3-8d; Then with 5～10% inoculum concentration access ferment tanks, tank pressure is 0.01～0.08MPa, cultivates 3-8d for 20-25 ℃, obtains fermentation Miao that mycelium and zymotic fluid mix; Then 35-50 ℃ of water-bath 10-40min, cross colloid mill, and 4000r/min is centrifugal, filters to obtain the bright fermentation mixed liquor of sepia clarification; Standby;
2) preparation of red date leaching liquor: red date is soaked, and the mass ratio of red date and water is 1: 10-15, and at 60-85 ℃, then lixiviate 3-5h filters to get the red date leaching liquor; Standby;
3) preparation of matrimony vine immersion fluid: matrimony vine is soaked, and matrimony vine and water quality ratio are 1: 35-50, and at 40-60 ℃, then lixiviate 30-60min filters to get the matrimony vine leaching liquor; Standby;
4) honeysuckle leaching liquor preparation: honeysuckle is soaked, and honeysuckle and water quality ratio are 1: 100-120 is the Microwave Extraction Apparatus of 120W with power, and extraction 10-20min filters to get Flos Lonicerae extractive solution; Standby;
5) Beverage Service: contain the 40-60mL cordyceps sinensis fermentation liquor by every 100mL beverage, 10-20mL matrimony vine leaching liquor, 2-8mL honeysuckle extract, 25-40mL red date leaching liquor, all the other add the water proportioning; Another auxiliary sucrose 0.2-0.4g, citric acid 0.05-0.2g, honey 0.2-0.8mL, Vc 0.02-0.05g, sodium sulfite 0.01-0.05g, the pectin 0.1-0.3g of adding stirs;
6) can: beverage product is divided install in corresponding bottle;
7) sterilization: beverage is at 120-135 ℃, and pressure is 1.000-2.110 * 10
5Pa is between 30-35 minute;
8) degassed: with the air in vacuum degassing machine eliminating beverage;
9) capping.
In above-mentioned raw materials, the Cordyceps militaris zymotic fluid contains the multiple lifes such as cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, nucleosides, sterol: active substances; human body is had multiple significant physiological function, have strengthen immunity, regulate endocrine, the different physiological roles such as antifatigue, anti-oxidant, anti-ageing, antibiotic, antitumor, anti-anoxic, calmness, hypoglycemic, protection liver kidney and respiratory system.The date leaching liquor contains a large amount of glucides, is mainly glucose, also contains fructose, sucrose, and compound sugar, araban and the galacturonan etc. that are comprised of glucose and fructose; And contain the multivitamins such as a large amount of vitamin Cs, riboflavin, thiamine, carrotene, niacin, and have stronger tonic effect, can improve immune function of human body, strengthen resistance against diseases.It is very high that the matrimony vine leaching liquor contains abundant LBP-X, fat, protein, free amino acid, taurine, betaine, vitamin B1, B2, E, C, particularly carotenoid content.Have filling liver kidney, beneficial vital essence, longue meat, improve complexion, make eye bright calm the nerves, dispel the wind control void, promote longevity, the effect of hard muscles and bones; In addition, also contain a large amount of mineral elements, as nutritional labelings such as potassium, sodium, calcium, magnesium, iron, copper, manganese, zinc, selenium., consumptive disease sallow to liver-kidney Yin deficiency, the deficiency of blood be smart loses, soreness and weakness of waist and knees, the disease such as have a dizzy spell have remarkable result.Flos Lonicerae extractive solution has clearing heat and detoxicating effect.The beverage that the reasonable coordination of above-mentioned several raw materials makes often ediblely can improve body immunity, strengthen muscle power, anti-ageing, nourishes the liver to improve visual acuity, tonifying spleen and stomach, nourishing blood and tranquilization, tonifying kidney and benefiting sperm, anti-sudden change, the auxiliary effect such as antitumor.
The invention has the beneficial effects as follows: the preparation method is simple to operate, the cycle is short; The composite beverage by fermenting Cordyceps militaris that makes is nutritious, have the body immunity of raising, strengthen muscle power, nourish the liver to improve visual acuity, tonifying spleen and stomach, nourishing blood and tranquilization, tonifying kidney and benefiting sperm, anti-ageing, anti-sudden change, the auxiliary effect such as antitumor.
The specific embodiment
Further illustrate the present invention below in conjunction with embodiment.
Embodiment 1
The preparation of Cordyceps militaris zymotic fluid: cordyceps militaris link bacterial strain PM14, SDAY cultivates on the inclined-plane, and 22 ℃, 5d; SDY shaking flask 120r/min, 22 ℃, 5d; 10% inoculum concentration access ferment tank is filled with and is pressed 0.03～0.05MPa, 120r/min, 22 ℃, 5d.Feeding obtains fermentation Miao of mycelium and zymotic fluid mixing, and 40 ℃ of water-bath 30min cross colloid mill, and 4000r/min is centrifugal, filters to obtain the bright zymotic fluid of sepia clarification.
Get respectively 10g red date, 5g matrimony vine, 1g honeysuckle and obtain jujube leaching liquor 120mL, matrimony vine leaching liquor 250mL, honeysuckle leaching liquor 120mL according to following solid-to-liquid ratio lixiviate.
Red date extracting condition: solid-to-liquid ratio: 1: 12, extraction temperature: 80 ℃, extraction time: 5h.
Matrimony vine extracting condition: solid-to-liquid ratio: 1: 45,50 ℃ of extraction temperatures, extraction time 60min.
Honeysuckle microwave abstracting condition: solid-to-liquid ratio 1: 120, power: 120W, extraction time: 10min.
Contain 50mL Cordyceps militaris zymotic fluid by every 100mL beverage, 15mL matrimony vine leaching liquor, 4mL honeysuckle extract, 30mL red date leaching liquor, add again sucrose 0.2g, citric acid 0.1g, honey 0.5mL, Vc 0.02g, sodium sulfite 0.01g, pectin O.14g, all the other add the water proportioning, stir, and obtain the bright liquid of sepia.Then at 121 ℃, pressure is 1.034 * 10
5Pa, high-temperature sterilization 30 minutes, air, capping through getting rid of with vacuum degassing machine in beverage obtain the functional health beverage that sepia is bright, sour-sweet taste, Chinese caterpillar fungus fragrance and honeysuckle fragrance that jujube juice and wolfberry juice are arranged are coordinated.
Claims (1)
1. the preparation method of a composite beverage by fermenting Cordyceps militaris, is characterized in that making as primary raw material take Cordyceps militaris, red date, matrimony vine, honeysuckle, and concrete steps are as follows:
1) preparation of Cordyceps militaris zymotic fluid: the Pm14 cordyceps militaris link bacterial strain, cultivate 3-8d for 20-25 ℃ on the SDAY slant medium; Then in the SDY culture medium with 100-140r/min, 20-25 ℃, shaking flask is cultivated 3-8d; Then with 5～10% inoculum concentration access ferment tanks, tank pressure is 0.01～0.08MPa, cultivates 3-8d for 20-25 ℃, obtains fermentation Miao that mycelium and zymotic fluid mix; Then 35-50 ℃ of water-bath 10-40min, cross colloid mill, and 4000r/min is centrifugal, filters to obtain the bright fermentation mixed liquor of sepia clarification; Standby;
2) preparation of red date leaching liquor: red date is soaked, and the mass ratio of red date and water is 1: 10-15, and at 60-85 ℃, then lixiviate 3-5h filters to get the red date leaching liquor; Standby;
3) preparation of matrimony vine leaching liquor: matrimony vine is soaked, and matrimony vine and water quality ratio are 1: 35-50, and at 40-60 ℃, then lixiviate 30-60min filters to get the matrimony vine leaching liquor; Standby;
4) honeysuckle leaching liquor preparation: honeysuckle is soaked, and honeysuckle and water quality ratio are 1: 100-120 is the Microwave Extraction Apparatus of 120W with power, and extraction 10-20min filters to get Flos Lonicerae extractive solution; Standby;
5) Beverage Service: contain the 40-60mL cordyceps sinensis fermentation liquor by every 100mL beverage, 10-20mL matrimony vine leaching liquor, 2-8mL honeysuckle extract, 25-40mL red date leaching liquor, all the other add the water proportioning; Another auxiliary sucrose 0.2-0.4g, citric acid 0.05-0.2g, honey 0.2-0.8mL, Vc 0.02-0.05g, sodium sulfite 0.01-0.05g, the pectin 0.1-0.3g of adding stirs;
6) can: beverage product is divided install in Packaging Bottle;
7) sterilization: with beverage 120-135 ℃, pressure 1.000-2.110 * 10
5Under Pa, sterilization is 30-35 minute;
8) degassed;
9) capping.
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