CN102389109A - Rice bran nutrient granule - Google Patents
Rice bran nutrient granule Download PDFInfo
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- CN102389109A CN102389109A CN2011103343936A CN201110334393A CN102389109A CN 102389109 A CN102389109 A CN 102389109A CN 2011103343936 A CN2011103343936 A CN 2011103343936A CN 201110334393 A CN201110334393 A CN 201110334393A CN 102389109 A CN102389109 A CN 102389109A
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Abstract
The invention discloses a rice bran nutrient granule which is made from filter, flavorings, antiseptics and rice bran nutrients. The parts by weight of the ingredients are as follows: 10 of rice bran nutrients, 5 to 20 of fillers, 1 to 2 of flavorings and 0.1 to 0.3 of antiseptics.
Description
Technical field
The invention belongs to food processing technology field, relate to a kind of rice bran nutrients electuary.
Background technology
Rice bran is the shell of grass paddy, be in the rice milling process by the mixture that grinds cortex down and meter embryo and crack rice on a small quantity, account for 5%~6% of paddy, it not only originates abundant, and comprehensive nutrition.Be rich in physiological activators such as unrighted acid, tocopherol, tocotrienols, lipopolysaccharides, edible fiber, squalene, γ-Hi-Z in the rice bran.These materials are for prevention people body-centered, cranial vascular disease, and are anticancer, and enhance immunity power reduces blood fat, and prevention constipation and obesity have significant function, are health food, medicine, the manufacturing important source material of chemical industry, receive in countries in the world
To extensively paying attention to.
In addition; Rice bran contains active very strong lipase, and this lipase can decompose grease contained in the rice bran very soon, and acid value is risen rapidly; And might stand the further oxidation (be commonly called as " breathe out become ") of lipoxygenase, produce a kind of unacceptable musty in the short period of time.Fresh rice bran, at normal temperatures several hours, its acid value can be risen to more than the 10mg KOH/g by 4mg KOH/g, and under 25 ℃ of temperature, the free fatty of rice bran (FFA) content increases to be about the 1%/h raising speed.Insect that is mingled with in the rice bran and the vital movement of microorganism also can be quickened the rice bran bad change of becoming sour.Therefore, necessary this lipase of passivation makes rice bran stable, and rice bran just can carry out deep development.
Though the total output of domestic rice bran is very big, because the deep process technology of paddy processing enterprise is immature, production scale is also little, adds fresh rice bran less stable, is difficult for storing and transportation, therefore is difficult to centralized production.At present, the rice bran effective rate of utilization is less than 20% still, and is most of as feed, even as waste material, serious waste of resources.
Find through retrieval, more about the deep process technology report of rice bran both at home and abroad.As: CN101301067A discloses the method that a kind of isolation technics is comprehensively extracted active matter in the rice bran; Extract the vegetative nerve acid amides earlier; Filter residue behind the utilization extraction vegetative nerve acid amides obtains rice bran protein through the heavy method of isoelectric point acid again; Through organic solvent fractional precipitation, ion exchange resin and organic solvent extractionprocess, obtain other active matters of rice bran protein, rice bran polysaccharide, phytic acid and 5 kinds of rice brans of rice bran oil respectively then.CN102178151A discloses a kind of method of utilizing the defatted rice bran coproduction to prepare rice bran dietary fiber and rice bran protein; Utilize defatted rice bran to be raw material; Handle degradation technique, centrifugation and isoelectric precipitation technology through alkali treatment, centrifugal treating and enzyme, realize the coproduction preparation of rice bran dietary fiber and rice bran protein.CN102144746A discloses the method that a kind of enzyme process prepares high nutritive rice bran, is to be raw material with stabilizing rice bran or the full composition of fresh rice bran, unites and uses cellulase, protease, phytase to handle, and makes high nutritive rice bran.
In a word; Deep processing to rice bran both at home and abroad mainly is conceived to the independent technology of extracting of nutritional labelings such as rice bran protein, rice bran polysaccharide and rice bran-fiber; These extraction and separation process all exist processing step complicacy, the demanding defective of production equipment, also are unfavorable for the effect of various nutrients in the meals health care in the collaborative performance rice bran simultaneously.
Summary of the invention
Deficiency in view of prior art the object of the present invention is to provide a kind of rice bran nutrients electuary.
A kind of rice bran nutrients electuary is prepared from filler, flavor enhancement, anticorrisive agent and said rice bran nutrients, and the weight portion content of each component is:
10 parts of rice bran nutrients
Filler 5-20 part
Flavor enhancement 1-2 part
Anticorrisive agent 0.1-0.3 part.
Preferably, described filler is a lactose or/and Icing Sugar, and described flavor enhancement is an essence, and described anticorrisive agent is a potassium sorbate.
Rice bran nutrients electuary of the present invention can be prepared from according to following method: with rice bran nutrients, filler, flavor enhancement and anticorrisive agent in 10: the ratio of 5-20: 1-2: 0.1-0.3 is mixed; Add suitable quantity of water, place the rotary propeller type mixer to mix.Then, move in the comminutor, make wet grain through 12 eye mesh screens, it is dry down that the particle after the moulding is placed on 45 ° hot blast, through screening.
The rice bran nutrients electuary of the present invention's preparation can adopt boiling water to take after mixing it with water.
Wherein, the preparation method of above-mentioned rice bran nutrients comprises the steps:
(1) 1000 parts of rice brans is cleaned, dries, pulverizes, cross 80 mesh sieves, handle 5-10min in 85 ℃ of damp and hot high-temperature steams, and in 10-15min, be warming up to 105 ℃ gradually, insulation processing 10-15min;
(2) with the benzinum of 2-3 times of weight of step (1) gained material adding, in 55-58 ℃ of stirring 0.5-0.8h, filter, filter residue is in 105 ℃ of dry 0.5-1.5h;
(3) aqueous hydrochloric acid solution with step (2) gained material and pH5.0-5.5 is that 1:8-10 mixes with the feed liquid weight ratio, adds 15-25 part combination enzyme A, keeps 38-45 ℃ and stirring reaction 1-2h, and the 3500-4000r/min centrifugal treating is collected supernatant;
(4) in step (3) gained supernatant, add 15-20 part combination enzyme B, keep 55-60 ℃ and stirring reaction 1-2h, the 3500-4000r/min centrifugal treating is collected supernatant;
(5) step (4) gained supernatant is concentrated through 50-55 ℃ of vacuum,, get rice bran nutrients then in subzero 20-25 ℃ freeze drying;
Wherein, the described combination enzyme of step (3) A is by pectase: phytase: cellulase=1:0.5-1:2-3 forms, and the described combination enzyme of step (4) B is by AMS: flavor protease=1:0.5-2 forms.
The preparation method of rice bran nutrients, can also realize like this:
The preparation method of described rice bran nutrients, the combination enzyme A in the step (3) is by pectase: phytase: cellulase=1:0.8:2 forms.
The preparation method of described rice bran nutrients, the combination enzyme B in the step (4) are by AMS: flavor protease=1:1.5 forms.
The preparation method of described rice bran nutrients, wherein the pectase enzyme activity unit is 50,000 U/g, and the phytase activity unit of force is 10,000 U/g, and the cellulose enzyme unit of activity is 100,000 U/g.
The preparation method of described rice bran nutrients, wherein the AMS enzyme activity unit is 4000U/g, the flavor protease enzyme activity unit is 15000U/g.
Compared with prior art, the rice bran nutrients electuary that the present invention relates to has following advantage and obvious improvement:
(1) a kind of rice bran nutrients electuary of the present invention contains multiple nutritional components such as rice bran protein, rice bran polysaccharide, solubility rice bran-fiber, phytic acid, mineral matter; Help these material preventions of collaborative performance or supplemental treatment human body cardiovascular and cerebrovascular disease, cancer; Enhance immunity power; Reduce blood fat, functions such as prevention constipation and obesity.
(2) this electuary preparation technology is simple, need not harsh reaction condition such as HTHP, to production equipment require lowly, be convenient to industrialized production.
The specific embodiment
Below through the embodiment form foregoing of the present invention is remake further detailed description; But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
The extraction of embodiment 1 rice bran nutrients separates
(1) gets fresh rice bran, through measuring rice bran total nitrogen content 14.4%, fat content 15.2%, moisture 18.5%; The 1000g rice bran is cleaned, dries, pulverizes, cross 80 mesh sieves, handle 6min in 85 ℃ of damp and hot high-temperature steams, and in 12min, be warming up to 105 ℃ gradually, insulation processing 15min;
(2) with the benzinum of step (1) gained material adding 2645g, in 55-58 ℃ of stirring 0.6h, filter, filter residue is in 105 ℃ of dry 1h;
(3) aqueous hydrochloric acid solution with step (2) gained material and pH5.5 is that 1:8 mixes with the feed liquid weight ratio, adds 20g combination enzyme A, keeps 40 ℃ and stirring reaction 1h, and the 3500r/min centrifugal treating is collected supernatant;
(4) in step (3) gained supernatant, add 15g combination enzyme B, keep 55 ℃ and stirring reaction 1-2h, the 3500-4000r/min centrifugal treating is collected supernatant;
(5) step (4) gained supernatant is concentrated through 55 ℃ of vacuum,, get the 432g rice bran nutrients then in subzero 20 ℃ of freeze dryings;
Wherein: the combination enzyme A of step (3) consists of: pectase (50,000 U/g) 7.4g, phytase (10,000 U/g) 5.9g, cellulase (100,000 U/g) 15g; The combination enzyme B of step (4) consists of: AMS (4000U/g) 6g, flavor protease (15000U/g) 9g.
The extraction of embodiment 2 rice bran nutrients separates
(1) gets fresh rice bran, through measuring rice bran total nitrogen content 14.6%, fat content 15.3%, moisture 18.3%; The 1000g rice bran is cleaned, dries, pulverizes, cross 80 mesh sieves, handle 8min in 85 ℃ of damp and hot high-temperature steams, and in 15min, be warming up to 105 ℃ gradually, insulation processing 15min;
(2) with the benzinum of step (1) gained material adding 2237g, in 55-58 ℃ of stirring 1h, filter, filter residue is in 105 ℃ of dry 1h;
(3) aqueous hydrochloric acid solution with step (2) gained material and pH5.0 is that 1:10 mixes with the feed liquid weight ratio, adds 20g combination enzyme A, keeps 45 ℃ and stirring reaction 1h, and the 4000r/min centrifugal treating is collected supernatant;
(4) in step (3) gained supernatant, add 15g combination enzyme B, keep 60 ℃ and stirring reaction 1-2h, the 4000r/min centrifugal treating is collected supernatant;
(5) step (4) gained supernatant is concentrated through 50 ℃ of vacuum,, get the 376g rice bran nutrients then in subzero 25 ℃ of freeze dryings;
Wherein: the combination enzyme A of step (3) consists of: pectase (50,000 U/g) 7.5g, phytase (10,000 U/g) 6g, cellulase (100,000 U/g) 15g; The combination enzyme B of step (4) consists of: AMS (4000U/g) 6g, flavor protease (15000U/g) 9g.
The extraction of comparative example's rice bran nutrients separates
Get the fresh rice bran of 500g, through measuring rice bran total nitrogen content 14.4%, fat content 15.3%, moisture 18.5%; Add 5kg water, stir, be heated to 40 ℃, add 2.5g food-grade cellulase (100,000 U/g), stir maintenance 24h.Add 5g food-grade phytase (10000U/g), stir maintenance 16h.Add 10g food-grade flavor protease (15000U/g), stirring keeps 6h, and the 4000r/min centrifugal treating is collected supernatant, and the gained supernatant concentrates through 50 ℃ of vacuum, then in subzero 25 ℃ of freeze dryings, gets rice bran nutrients.
The preparation of embodiment 3 rice bran nutrients electuaries
With the rice bran nutrients of embodiment 1 preparation, and filler, flavor enhancement and anticorrisive agent were in 10: 10: 1: 0.1 ratio is mixed, and the adding suitable quantity of water places the rotary propeller type mixer to mix.Then, move in the comminutor, make wet grain through 12 eye mesh screens, it is dry down that the particle after the moulding is placed on 45 ° hot blast, through screening.
Wherein, filler is made up of the mixture of lactose: Icing Sugar=1:4 (weight ratio), and flavor enhancement is an essence, and anticorrisive agent is a potassium sorbate.
Rice bran nutrients in the embodiment 4 rice bran nutrients electuaries is to the supplemental treatment effect of liver cancer mouse model
The healthy kunming mice of SPF level, male and female half and half, 6 age in week, heavily l8g-20g.Get exponential phase H22 (S type) HCC of in vitro culture, adjustment oncocyte concentration is 1 * 10
7/ mL.Under aseptic condition, it is subcutaneous that oncocyte is inoculated in the right armpit of mouse, and inoculum concentration is that only (cell number is 2 * 10 to 0.2ml/
6/ only).Whole seeded process must be carried out with sterile working in aseptic cover, accomplishes inoculation in the 1h.Treat that subcutaneous transplantation knurl volume reaches 60mm
3During the left and right sides (about lOd), model manufacturing success.Liver cancer mouse is divided into 4 groups by knurl volume and mice with tumor body weight homeostatic principle: model control group, endoxan group, test I group and test II group, 15 every group.Each group is by being tried thing like following table 1:
What table 1 was respectively organized liver cancer mouse is tried thing and therapeutic modality
Group | Tried thing and therapeutic modality |
Model control group | The physiological saline of filling stomach equivalent 1 time/day gives 40 altogether. |
The endoxan group | Tail vein injection 25mg/kg endoxan, the next day administration 1 time, amount to 12 times. |
Test I group | Tail vein injection 25mg/kg endoxan, the next day administration 1 time, amount to 12 times; Irritate the rice bran nutrients of comparative example's preparation that stomach gives simultaneously, dosage is 8g/kg, 1 time/day, gives 40 altogether. |
Test II group | Tail vein injection 25mg/kg endoxan, the next day administration 1 time, amount to 12 times; Irritate the rice bran nutrients of the embodiment of the invention 1 preparation that stomach gives simultaneously, dosage is 8g/kg, 1 time/day, gives 40 altogether. |
Last is tried thing, and mouse is put to death in dislocation behind 48h, and the excision transplantable tumor takes by weighing knurl and weighs.Heavy inhibiting rate (%) IR of knurl=(1-administration group knurl weight-average value/model control group knurl weight-average value) * 100%.Relatively embody medicine to the liver cancer mouse model preventive and therapeutic effect through what knurl weighed.(x ± s) expression adopts SPSS15.0 software to carry out variance analysis to data with mean ± standard deviation.
Table 2 rice bran nutrients is to the supplemental treatment effect of liver cancer mouse model
Group | Sample size (beginning/end) | Knurl heavy (g) | Average tumour inhibiting rate (%) |
Model control group | 15/14 | 2.93±0.61 | ? |
The endoxan group | 15/11 | 1.76±0.68 ** | 40.0 |
Test I group | 15/13 | 1.86±0.72 ** | 36.5 |
Test II group | 15/15 | 1.14±0.59 **¥# | 61.1 |
Compare with model control group,
* P<0.01;
Compare with the endoxan group,
$ P<0.05;
Compare with test I group,
# P<0.05.
Result of the test shows (referring to table 2): compare with model control group, each treatment group to the preventive and therapeutic effect of rat liver cancer model all have utmost point significant difference (
P<0.01), this explanation endoxan has extremely significant curative effect at the treatment liver cancer.In addition; Test II group (endoxan+rice bran nutrients of the present invention) is compared aspect the tumour inhibiting rate with the endoxan group has significant difference; And 4 of the dead mouses of endoxan group; This explains that rice bran nutrients of the present invention can reach significant Synergistic anti-cancer result of treatment by the combined with chemotherapy medicine, and can reduce the death rate of mice with tumor.The 3rd; Test II group (endoxan+rice bran nutrients of the present invention) is compared aspect the tumour inhibiting rate with test I group (endoxan+contrast rice bran nutrients) has significant difference; And 2 of the dead mouses of test I group, this explanation the present invention is in the auxiliary cancer or anticancer and keep aspect the survival rate of mice with tumor significantly because prior art that presses down.
Claims (2)
1. rice bran nutrients electuary, it is characterized in that: be prepared from filler, flavor enhancement, anticorrisive agent and said rice bran nutrients, the weight portion content of each component is:
10 parts of rice bran nutrients
Filler 5-20 part
Flavor enhancement 1-2 part
Anticorrisive agent 0.1-0.3 part.
2. a kind of rice bran nutrients electuary according to claim 1 is characterized in that: described filler is a lactose or/and Icing Sugar, and described flavor enhancement is an essence, and described anticorrisive agent is a potassium sorbate.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107373636A (en) * | 2017-07-21 | 2017-11-24 | 安康世茂生物科技有限公司 | A kind of health products of cancer-resisting |
CN109198430A (en) * | 2018-08-17 | 2019-01-15 | 湖南米珍宝生物高科技有限公司 | The method that nutrition additive and brown rice skin powder are processed as nutrition additive |
Citations (3)
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CN1327764A (en) * | 2000-06-08 | 2001-12-26 | 无锡轻工大学 | Method for producing health-care food containing rice bran nutrients |
CN1328830A (en) * | 2000-06-16 | 2002-01-02 | 赵今月 | Pueraria root soluble granules |
CN101278712A (en) * | 2008-05-15 | 2008-10-08 | 江苏双兔食品股份有限公司 | Rice bran nutriment and method for separating rice bran nutrition fibre with high-efficiency |
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2011
- 2011-10-29 CN CN2011103343936A patent/CN102389109B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1327764A (en) * | 2000-06-08 | 2001-12-26 | 无锡轻工大学 | Method for producing health-care food containing rice bran nutrients |
CN1328830A (en) * | 2000-06-16 | 2002-01-02 | 赵今月 | Pueraria root soluble granules |
CN101278712A (en) * | 2008-05-15 | 2008-10-08 | 江苏双兔食品股份有限公司 | Rice bran nutriment and method for separating rice bran nutrition fibre with high-efficiency |
Non-Patent Citations (2)
Title |
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毛金水等: "米糠营养素提取的研究与生产应用", 《粮食储藏》 * |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107373636A (en) * | 2017-07-21 | 2017-11-24 | 安康世茂生物科技有限公司 | A kind of health products of cancer-resisting |
CN109198430A (en) * | 2018-08-17 | 2019-01-15 | 湖南米珍宝生物高科技有限公司 | The method that nutrition additive and brown rice skin powder are processed as nutrition additive |
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