CN102363009B - Traditional Chinese medicine composition with yin nourishing, yang assisting, vital essence reinforcing efficacies - Google Patents
Traditional Chinese medicine composition with yin nourishing, yang assisting, vital essence reinforcing efficacies Download PDFInfo
- Publication number
- CN102363009B CN102363009B CN201110324167A CN201110324167A CN102363009B CN 102363009 B CN102363009 B CN 102363009B CN 201110324167 A CN201110324167 A CN 201110324167A CN 201110324167 A CN201110324167 A CN 201110324167A CN 102363009 B CN102363009 B CN 102363009B
- Authority
- CN
- China
- Prior art keywords
- weight portions
- radix
- weight
- fructus
- rhizoma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003814 drug Substances 0.000 title claims abstract description 137
- 239000000203 mixture Substances 0.000 title claims abstract description 76
- 230000003014 reinforcing effect Effects 0.000 title abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract description 48
- 230000000694 effects Effects 0.000 claims abstract description 47
- 239000000463 material Substances 0.000 claims abstract description 25
- 210000000582 semen Anatomy 0.000 claims description 172
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 99
- 239000000843 powder Substances 0.000 claims description 78
- 229940079593 drug Drugs 0.000 claims description 77
- 238000000034 method Methods 0.000 claims description 55
- 238000012360 testing method Methods 0.000 claims description 47
- 238000002156 mixing Methods 0.000 claims description 45
- 239000006187 pill Substances 0.000 claims description 43
- 239000009636 Huang Qi Substances 0.000 claims description 40
- 239000010135 fructus aurantii immaturus Substances 0.000 claims description 40
- 239000013558 reference substance Substances 0.000 claims description 40
- 238000012258 culturing Methods 0.000 claims description 38
- 241000208340 Araliaceae Species 0.000 claims description 37
- 241000190633 Cordyceps Species 0.000 claims description 37
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 37
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 37
- 235000008434 ginseng Nutrition 0.000 claims description 37
- 241000965254 Apostichopus japonicus Species 0.000 claims description 36
- 241000756943 Codonopsis Species 0.000 claims description 36
- 241000180649 Panax notoginseng Species 0.000 claims description 36
- 235000003143 Panax notoginseng Nutrition 0.000 claims description 36
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims description 36
- 241001506047 Tremella Species 0.000 claims description 36
- 108010052008 colla corii asini Proteins 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 238000001035 drying Methods 0.000 claims description 28
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 27
- 230000008569 process Effects 0.000 claims description 27
- 241000005787 Cistanche Species 0.000 claims description 25
- 239000000796 flavoring agent Substances 0.000 claims description 25
- 235000019634 flavors Nutrition 0.000 claims description 25
- 239000000706 filtrate Substances 0.000 claims description 22
- 241000252335 Acipenser Species 0.000 claims description 21
- 240000001008 Dimocarpus longan Species 0.000 claims description 21
- 235000000235 Euphoria longan Nutrition 0.000 claims description 21
- 241000237524 Mytilus Species 0.000 claims description 21
- 241001534869 Terminalia Species 0.000 claims description 21
- 210000004712 air sac Anatomy 0.000 claims description 21
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 20
- 235000012907 honey Nutrition 0.000 claims description 17
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 12
- 239000004575 stone Substances 0.000 claims description 12
- 239000002671 adjuvant Substances 0.000 claims description 10
- 238000003556 assay Methods 0.000 claims description 10
- 235000021419 vinegar Nutrition 0.000 claims description 10
- 239000000052 vinegar Substances 0.000 claims description 10
- JAYVHSBYKLLDJC-DSNJPTTOSA-N (E)-2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C(O)C=C(O)C=C1\C=C\C1=CC=C(O)C=C1 JAYVHSBYKLLDJC-DSNJPTTOSA-N 0.000 claims description 9
- 238000007796 conventional method Methods 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- 239000000835 fiber Substances 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 8
- 238000004809 thin layer chromatography Methods 0.000 claims description 8
- 238000005303 weighing Methods 0.000 claims description 8
- PBCJIPOGFJYBJE-UHFFFAOYSA-N acetonitrile;hydrate Chemical compound O.CC#N PBCJIPOGFJYBJE-UHFFFAOYSA-N 0.000 claims description 7
- 239000002775 capsule Substances 0.000 claims description 7
- 239000000945 filler Substances 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 claims description 7
- 239000000377 silicon dioxide Substances 0.000 claims description 7
- 238000011003 system suitability test Methods 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000008187 granular material Substances 0.000 claims description 6
- 239000007901 soft capsule Substances 0.000 claims description 6
- 241000283898 Ovis Species 0.000 claims description 5
- 238000010791 quenching Methods 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 239000004576 sand Substances 0.000 claims description 5
- 239000003826 tablet Substances 0.000 claims description 5
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 claims description 4
- 229960002327 chloral hydrate Drugs 0.000 claims description 4
- SKCNIGRBPJIUBQ-UHFFFAOYSA-N chloroform;ethyl acetate Chemical compound ClC(Cl)Cl.CCOC(C)=O SKCNIGRBPJIUBQ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003480 eluent Substances 0.000 claims description 4
- 210000002615 epidermis Anatomy 0.000 claims description 4
- OAYLNYINCPYISS-UHFFFAOYSA-N ethyl acetate;hexane Chemical compound CCCCCC.CCOC(C)=O OAYLNYINCPYISS-UHFFFAOYSA-N 0.000 claims description 4
- 230000001788 irregular Effects 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- CASUWPDYGGAUQV-UHFFFAOYSA-M potassium;methanol;hydroxide Chemical compound [OH-].[K+].OC CASUWPDYGGAUQV-UHFFFAOYSA-M 0.000 claims description 4
- 210000003491 skin Anatomy 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims description 4
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 4
- 235000012141 vanillin Nutrition 0.000 claims description 4
- 239000002023 wood Substances 0.000 claims description 4
- 238000012850 discrimination method Methods 0.000 claims description 3
- 230000003712 anti-aging effect Effects 0.000 claims description 2
- 239000008280 blood Substances 0.000 abstract description 17
- 210000002966 serum Anatomy 0.000 abstract description 17
- 210000004185 liver Anatomy 0.000 abstract description 12
- 210000003734 kidney Anatomy 0.000 abstract description 11
- 210000000952 spleen Anatomy 0.000 abstract description 10
- 230000009182 swimming Effects 0.000 abstract description 10
- 102100021022 Gastrin Human genes 0.000 abstract description 9
- 108010052343 Gastrins Proteins 0.000 abstract description 9
- 229920002527 Glycogen Polymers 0.000 abstract description 9
- 241000699670 Mus sp. Species 0.000 abstract description 9
- AOXOCDRNSPFDPE-UKEONUMOSA-N chembl413654 Chemical compound C([C@H](C(=O)NCC(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](C)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H]1N(CCC1)C(=O)CNC(=O)[C@@H](N)CCC(O)=O)C1=CC=C(O)C=C1 AOXOCDRNSPFDPE-UKEONUMOSA-N 0.000 abstract description 9
- 230000007812 deficiency Effects 0.000 abstract description 9
- 229940096919 glycogen Drugs 0.000 abstract description 9
- 238000002474 experimental method Methods 0.000 abstract description 8
- 230000001965 increasing effect Effects 0.000 abstract description 6
- 206010033557 Palpitations Diseases 0.000 abstract description 4
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 abstract description 4
- 230000001976 improved effect Effects 0.000 abstract description 4
- 206010022437 insomnia Diseases 0.000 abstract description 4
- 230000036565 night sweats Effects 0.000 abstract description 4
- 206010029410 night sweats Diseases 0.000 abstract description 4
- 238000005728 strengthening Methods 0.000 abstract description 4
- 206010010774 Constipation Diseases 0.000 abstract description 3
- 206010012735 Diarrhoea Diseases 0.000 abstract description 3
- 208000019255 Menstrual disease Diseases 0.000 abstract description 3
- 230000002269 spontaneous effect Effects 0.000 abstract description 3
- 210000001835 viscera Anatomy 0.000 abstract description 3
- 206010029446 nocturia Diseases 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 208000000044 Amnesia Diseases 0.000 abstract 1
- 208000031091 Amnestic disease Diseases 0.000 abstract 1
- 206010006784 Burning sensation Diseases 0.000 abstract 1
- 240000008574 Capsicum frutescens Species 0.000 abstract 1
- 206010034568 Peripheral coldness Diseases 0.000 abstract 1
- 206010047924 Wheezing Diseases 0.000 abstract 1
- 208000031975 Yang Deficiency Diseases 0.000 abstract 1
- 230000006986 amnesia Effects 0.000 abstract 1
- 230000036528 appetite Effects 0.000 abstract 1
- 235000019789 appetite Nutrition 0.000 abstract 1
- 208000002173 dizziness Diseases 0.000 abstract 1
- 235000007183 paleface Nutrition 0.000 abstract 1
- 208000024891 symptom Diseases 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 230000001256 tonic effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 66
- 239000002994 raw material Substances 0.000 description 43
- 210000004027 cell Anatomy 0.000 description 17
- 241000700159 Rattus Species 0.000 description 13
- 210000004369 blood Anatomy 0.000 description 13
- 241000721047 Danaus plexippus Species 0.000 description 12
- 239000013641 positive control Substances 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 10
- 230000002440 hepatic effect Effects 0.000 description 9
- 239000008194 pharmaceutical composition Substances 0.000 description 9
- 230000003203 everyday effect Effects 0.000 description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 6
- 210000002784 stomach Anatomy 0.000 description 6
- 230000007423 decrease Effects 0.000 description 5
- 230000027939 micturition Effects 0.000 description 5
- 241000700157 Rattus norvegicus Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 208000012886 Vertigo Diseases 0.000 description 4
- 230000032683 aging Effects 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 210000004072 lung Anatomy 0.000 description 4
- 208000011580 syndromic disease Diseases 0.000 description 4
- 231100000889 vertigo Toxicity 0.000 description 4
- 206010063659 Aversion Diseases 0.000 description 3
- 206010013954 Dysphoria Diseases 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 3
- 206010037660 Pyrexia Diseases 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000005252 bulbus oculi Anatomy 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000006196 drop Substances 0.000 description 3
- 210000003414 extremity Anatomy 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 description 3
- 230000035807 sensation Effects 0.000 description 3
- 235000019615 sensations Nutrition 0.000 description 3
- 238000010254 subcutaneous injection Methods 0.000 description 3
- 239000007929 subcutaneous injection Substances 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- 238000012449 Kunming mouse Methods 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 206010061428 decreased appetite Diseases 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000004064 dysfunction Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 210000003127 knee Anatomy 0.000 description 2
- 210000005228 liver tissue Anatomy 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- XOFYZVNMUHMLCC-ZPOLXVRWSA-N prednisone Chemical compound O=C1C=C[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 XOFYZVNMUHMLCC-ZPOLXVRWSA-N 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 241000590988 Danainae Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010059013 Nocturnal emission Diseases 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 208000031971 Yin Deficiency Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004103 aerobic respiration Effects 0.000 description 1
- 235000019606 astringent taste Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000000550 effect on aging Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 229960004618 prednisone Drugs 0.000 description 1
- 229940069514 rhubarb preparation Drugs 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000021595 spermatogenesis Effects 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 210000001113 umbilicus Anatomy 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a traditional Chinese medicine composition with yin nourishing, yang assisting, vital essence reinforcing, and primordial qi strengthening efficacies, a preparation method thereof, and a purpose thereof. The traditional Chinese medicine composition provided by the invention is composed of 59 medicine materials. The medicine materials provide synergetic effects of yin nourishing, yang assisting, vital essence reinforcing, and primordial qi strengthening. The traditional Chinese medicine composition is used for treating viscera functional disturbance, qi-blood deficiency and yin-yang deficiency with symptoms of pale face, severe palpitation, fatigue, easy wheeze, frequent long breathe, chilly feeling, cold extremities, amnesia, insomnia, dreaminess, burning sensation of five centers, night sweat, spontaneous perspiration, dizziness, poor appetite, loose stool, constipation, menoxenia, and frequent urinate or frequent nocturia. The traditional Chinese medicine composition contains a plurality of medicine materials, but the medicine materials are not disordered. With the formula, yin-nourishing medicine materials assists in nourishing yin with the aid of yang, and yang-nourishing medicine materials assists in nourishing yang with the aid of yin. The traditional Chinese medicine composition provided by the invention can be a model for yin-yang regulating, vital essence reinforcing, primordial qi strengthening, spleen and kidney covering, and heart and kidney balancing tonic preparations. As results of experiments, with the medicine composition provided by the invention, SDH activity can be substantially improved; LDH level can be reduced; serum gastrin content of rat with spleen qi deficiency can be increased; liver glycogen content of mice after weight-bearing swimming is increased; and serum LA level of mice after weight-bearing swimming is reduced. Thus, the traditional Chinese medicine composition provides better effects in age resisting, yang invigorating, and qi benefiting.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, particularly a kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect.
Background technology
" negative and positive of qi and blood loses empty " clinical show as more: internal organs dysfunction, negative and positive of qi and blood all decreases, lusterless complexion, severe palpitation, the lazy speech of breathing hard; Spiritlessness and weakness, moving then work is breathed heavily, and likes out long gas, aversion to cold and cold limbs, forgetful insomnia; Dreaminess, dysphoria with feverish sensation in the chest palms and soles, night sweat or spontaneous perspiration, the vertigo.Inappetence, loose stool or constipation, menoxenia, frequent micturition or frequent urination at night.
" negative and positive of qi and blood loses empty " also can cause the generation of multiple disease, and be especially in the majority with chronic disease, like hypertension, coronary heart disease, hyperlipemia, diabetes, sub-health state etc.Therefore very big to human health risk, the development and utilization of demanding the association area active drug urgently.
Summary of the invention
The object of the invention is to provide a kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect; Another purpose of the present invention is to provide the method for preparing of this Chinese medicine composition; The object of the invention also is to provide the purposes of this Chinese medicine composition.
The present invention seeks to realize through following technical scheme:
A kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect, its 59 flavor crude drug composition and weight proportion are following:
Radix Ginseng 7.5-21 weight portion; Radix Rehmanniae Preparata 27-45 weight portion; Cornu Cervi 40-64.8 weight portion; Cordyceps 25-50 weight portion; Radix Polygoni Multiflori 108-288 weight portion; Carapax Et Plastrum Testudinis 36-72 weight portion; Fructus Corni 18-48 weight portion; Fructus Lycii 144-288 weight portion; Radix Astragali 36-108 weight portion; Rhizoma Atractylodis Macrocephalae 36-108 weight portion; Ligamentum cervi 1.875-12 weight portion; Semen Cuscutae 100-168 weight portion; Fructus Ligustri Lucidi 8-35 weight portion; Herba Ecliptae 20-50 weight portion; Tremella 24-48 weight portion; Mytilus 48-96 weight portion; Radix Bupleuri 2-16 weight portion; Fructus Aurantii 4-21 weight portion; Pericarpium Citri Reticulatae 2-16 weight portion; Lignum Aquilariae Resinatum 2-16 weight portion; Calculus Bovis 3-16 weight portion; Radix Scutellariae 4-20 weight portion; Rhizoma Coptidis 4-20 weight portion; Cortex Phellodendri 4-25 weight portion; Arillus Longan 24-54 weight portion; Rhizoma Acori Graminei 4-20 weight portion; Radix Polygalae 8-25 weight portion; Radix Salviae Miltiorrhizae 60-112 weight portion; Radix Notoginseng 4-20 weight portion; Fructus Jujubae 34.2-64.8 weight portion; Radix Glycyrrhizae 57.6-120 weight portion; Radix Codonopsis 8-30 weight portion; Fructus Schisandrae Chinensis 8-30 weight portion; Radix Angelicae Sinensis 18-48 weight portion; Rhizoma Polygonati Odorati 36-96 weight portion; Pseudobulbus Bletillae (Rhizoma Bletillae) 18-48 weight portion; Semen pini koraiensis 36-96 weight portion; Semen Nelumbinis 36-96 weight portion; Semen Juglandis 36-120 weight portion; Herba Epimedii 8-30 weight portion; Rhizoma Dioscoreae 36-96 weight portion; Fructus Amomi 2.25-21 weight portion; Semen Astragali Complanati 72-192 weight portion; Fructus Aurantii Immaturus 4.5-21 weight portion; Radix Morindae Officinalis 8-30 weight portion; Air Bladder pseudosciaenae seu Acipenser 18-48 weight portion; Stichopus japonicus 18-48 weight portion; Radix Rehmanniae 18-48 weight portion; Cortex Cinnamomi 4.5-21 weight portion; Rhizoma Polygonati 36-120 weight portion; Fructus Psoraleae 8-30 weight portion; Radix Paeoniae Alba 36-96 weight portion; Fructus Mume 8-30 weight portion; Semen Platycladi 36-120 weight portion; Colla Corii Asini 8-30 weight portion; Terminalia flesh 8-30 weight portion; Herba Cistanches 18-48 weight portion; Fructus Litseae 4.5-21 weight portion; Poria 50-90 weight portion.
A kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect, its 59 flavor crude drug composition and weight proportion are preferably following:
Radix Ginseng 9 weight portions, Radix Rehmanniae Preparata 27 weight portions, Cornu Cervi 45 weight portions,
Cordyceps 27 weight portions, Radix Polygoni Multiflori 207 weight portions, Carapax Et Plastrum Testudinis 45 weight portions,
Fructus Corni 27 weight portions, Fructus Lycii 207 weight portions, the Radix Astragali 63 weight portions,
The Rhizoma Atractylodis Macrocephalae 63 weight portions, Ligamentum cervi 2 weight portions, Semen Cuscutae 135 weight portions,
Fructus Ligustri Lucidi 9 weight portions, Herba Ecliptae 27 weight portions, Tremella 27 weight portions,
Mytilus 63 weight portions, Radix Bupleuri 4 weight portions, Fructus Aurantii 5 weight portions,
Pericarpium Citri Reticulatae 4 weight portions, Lignum Aquilariae Resinatum 4 weight portions, Calculus Bovis 4 weight portions,
Radix Scutellariae 5 weight portions, Rhizoma Coptidis 5 weight portions, Cortex Phellodendri, 4.5 weight portions,
Arillus Longan 27 weight portions, Rhizoma Acori Graminei 5 weight portions, Radix Polygalae 9 weight portions,
Radix Salviae Miltiorrhizae 63 weight portions, Radix Notoginseng 5 weight portions, Fructus Jujubae 45 weight portions,
Radix Glycyrrhizae 63 weight portions, Radix Codonopsis 9 weight portions, Fructus Schisandrae Chinensis 9 weight portions,
Radix Angelicae Sinensis 27 weight portions, Rhizoma Polygonati Odorati 63 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 27 weight portions,
Semen pini koraiensis 63 weight portions, Semen Nelumbinis 63 weight portions, Semen Juglandis 63 weight portions
Herba Epimedii 9 weight portions, Rhizoma Dioscoreae 63 weight portions, Fructus Amomi 4 weight portions
Semen Astragali Complanati 135 weight portions, Fructus Aurantii Immaturus 5 weight portions, Radix Morindae Officinalis 9 weight portions,
Air Bladder pseudosciaenae seu Acipenser 27 weight portions, Stichopus japonicus 27 weight portions, Radix Rehmanniae 27 weight portions,
Cortex Cinnamomi 5 weight portions, Rhizoma Polygonati 63 weight portions, Fructus Psoraleae 9 weight portions,
The Radix Paeoniae Alba 63 weight portions, Fructus Mume 9 weight portions, Semen Platycladi 63 weight portions,
Colla Corii Asini 9 weight portions, terminalia flesh 9 weight portions, Herba Cistanches 27 weight portions
Fructus Litseae 5 weight portions, Poria 63 weight portions.
A kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect, its 59 flavor crude drug composition and weight proportion are preferably following:
Radix Ginseng 9 weight portions, Radix Rehmanniae Preparata 36 weight portions, Cornu Cervi 54 weight portions,
Cordyceps 36 weight portions, Radix Polygoni Multiflori 216 weight portions, Carapax Et Plastrum Testudinis 54 weight portions,
Fructus Corni 36 weight portions, Fructus Lycii 216 weight portions, the Radix Astragali 72 weight portions,
The Rhizoma Atractylodis Macrocephalae 72 weight portions, Ligamentum cervi 2.25 weight portions, Semen Cuscutae 144 weight portions,
Fructus Ligustri Lucidi 18 weight portions, Herba Ecliptae 36 weight portions, Tremella 36 weight portions,
Mytilus 72 weight portions, Radix Bupleuri 4.5 weight portions, Fructus Aurantii 9 weight portions,
Pericarpium Citri Reticulatae 4.5 weight portions, Lignum Aquilariae Resinatum 4.5 weight portions, Calculus Bovis 4.5 weight portions,
Radix Scutellariae 9 weight portions, Rhizoma Coptidis 9 weight portions, Cortex Phellodendri 13.5 weight portions,
Arillus Longan 36 weight portions, Rhizoma Acori Graminei 9 weight portions, Radix Polygalae 18 weight portions,
Radix Salviae Miltiorrhizae 72 weight portions, Radix Notoginseng 9 weight portions, Fructus Jujubae 54 weight portions,
Radix Glycyrrhizae 72 weight portions, Radix Codonopsis 18 weight portions, Fructus Schisandrae Chinensis 18 weight portions,
Radix Angelicae Sinensis 36 weight portions, Rhizoma Polygonati Odorati 72 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 36 weight portions,
Semen pini koraiensis 72 weight portions, Semen Nelumbinis 72 weight portions, Semen Juglandis 72 weight portions
Herba Epimedii 18 weight portions, Rhizoma Dioscoreae 72 weight portions, Fructus Amomi 4.5 weight portions
Semen Astragali Complanati 144 weight portions, Fructus Aurantii Immaturus 9 weight portions, Radix Morindae Officinalis 18 weight portions,
Air Bladder pseudosciaenae seu Acipenser 36 weight portions, Stichopus japonicus 36 weight portions, Radix Rehmanniae 36 weight portions,
Cortex Cinnamomi 9 weight portions, Rhizoma Polygonati 72 weight portions, Fructus Psoraleae 18 weight portions,
The Radix Paeoniae Alba 72 weight portions, Fructus Mume 18 weight portions, Semen Platycladi 72 weight portions,
Colla Corii Asini 18 weight portions, terminalia flesh 18 weight portions, Herba Cistanches 36 weight portions
Fructus Litseae 9 weight portions, Poria 72 weight portions.
A kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect, its 59 flavor crude drug composition and weight proportion are preferably following:
Radix Ginseng 9 weight portions, Radix Rehmanniae Preparata 45 weight portions, Cornu Cervi 63 weight portions,
Cordyceps 45 weight portions, Radix Polygoni Multiflori 225 weight portions, Carapax Et Plastrum Testudinis 63 weight portions,
Fructus Corni 45 weight portions, Fructus Lycii 225 weight portions, the Radix Astragali 81 weight portions,
The Rhizoma Atractylodis Macrocephalae 81 weight portions, Ligamentum cervi 11.25 weight portions, Semen Cuscutae 153 weight portions,
Fructus Ligustri Lucidi 27 weight portions, Herba Ecliptae 45 weight portions, Tremella 45 weight portions,
Mytilus 81 weight portions, Radix Bupleuri 13.5 weight portions, Fructus Aurantii 18 weight portions,
Pericarpium Citri Reticulatae 13.5 weight portions, Lignum Aquilariae Resinatum 13.5 weight portions, Calculus Bovis 13.5 weight portions,
Radix Scutellariae 18 weight portions, Rhizoma Coptidis 18 weight portions, Cortex Phellodendri 22.5 weight portions,
Arillus Longan 45 weight portions, Rhizoma Acori Graminei 18 weight portions, Radix Polygalae 27 weight portions,
Radix Salviae Miltiorrhizae 81 weight portions, Radix Notoginseng 18 weight portions, Fructus Jujubae 63 weight portions,
Radix Glycyrrhizae 81 weight portions, Radix Codonopsis 27 weight portions, Fructus Schisandrae Chinensis 27 weight portions,
Radix Angelicae Sinensis 45 weight portions, Rhizoma Polygonati Odorati 81 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 45 weight portions,
Semen pini koraiensis 81 weight portions, Semen Nelumbinis 81 weight portions, Semen Juglandis 81 weight portions
Herba Epimedii 27 weight portions, Rhizoma Dioscoreae 81 weight portions, Fructus Amomi 13.5 weight portions
Semen Astragali Complanati 153 weight portions, Fructus Aurantii Immaturus 18 weight portions, Radix Morindae Officinalis 27 weight portions,
Air Bladder pseudosciaenae seu Acipenser 45 weight portions, Stichopus japonicus 45 weight portions, Radix Rehmanniae 45 weight portions,
Cortex Cinnamomi 18 weight portions, Rhizoma Polygonati 81 weight portions, Fructus Psoraleae 27 weight portions,
The Radix Paeoniae Alba 81 weight portions, Fructus Mume 27 weight portions, Semen Platycladi 81 weight portions,
Colla Corii Asini 27 weight portions, terminalia flesh 27 weight portions, Herba Cistanches 45 weight portions
Fructus Litseae 18 weight portions, Poria 81 weight portions.
A kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect, its 59 flavor crude drug composition and weight proportion are preferably following:
Radix Ginseng 20 weight portions; Radix Rehmanniae Preparata 28 weight portions; Cornu Cervi 64 weight portions; Cordyceps 26 weight portions; Radix Polygoni Multiflori 280 weight portions; Carapax Et Plastrum Testudinis 36 weight portions; Fructus Corni 47 weight portions; Fructus Lycii 145 weight portions; The Radix Astragali 106 weight portions; The Rhizoma Atractylodis Macrocephalae 37 weight portions; Ligamentum cervi 12 weight portions; Semen Cuscutae 105 weight portions; Fructus Ligustri Lucidi 34 weight portions; Herba Ecliptae 22 weight portions; Tremella 46 weight portions; Mytilus 49 weight portions; Radix Bupleuri 10 weight portions; Fructus Aurantii 20 weight portions; Pericarpium Citri Reticulatae 8 weight portions; Lignum Aquilariae Resinatum 4 weight portions; Calculus Bovis 15 weight portions; Radix Scutellariae 18 weight portions; Rhizoma Coptidis 5 weight portions; Cortex Phellodendri 19 weight portions; Arillus Longan 26 weight portions; Rhizoma Acori Graminei 18 weight portions; Radix Polygalae 10 weight portions; Radix Salviae Miltiorrhizae 90 weight portions; Radix Notoginseng 8 weight portions; Fructus Jujubae 60 weight portions; Radix Glycyrrhizae 59 weight portions; Radix Codonopsis 25 weight portions; Fructus Schisandrae Chinensis 10 weight portions; Radix Angelicae Sinensis 45 weight portions; Rhizoma Polygonati Odorati 39 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 45 weight portions; Semen pini koraiensis 38 weight portions; Semen Nelumbinis 90 weight portions; Semen Juglandis 38 weight portions; Herba Epimedii 15 weight portions; Rhizoma Dioscoreae 90 weight portions; Fructus Amomi 10 weight portions; Semen Astragali Complanati 180 weight portions; Fructus Aurantii Immaturus 6 weight portions; Radix Morindae Officinalis 18 weight portions; Air Bladder pseudosciaenae seu Acipenser 20 weight portions; Stichopus japonicus 47 weight portions; Radix Rehmanniae 30 weight portions; Cortex Cinnamomi 20 weight portions; Rhizoma Polygonati 100 weight portions; Fructus Psoraleae 9 weight portions; The Radix Paeoniae Alba 90 weight portions; Fructus Mume 10 weight portions; Semen Platycladi 110 weight portions; Colla Corii Asini 11 weight portions; Terminalia flesh 29 weight portions; Herba Cistanches 20 weight portions; Fructus Litseae 20 weight portions; Poria 55 weight portions.
A kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect, its 59 flavor crude drug composition and weight proportion are preferably following:
Radix Ginseng 8 weight portions; Radix Rehmanniae Preparata 45 weight portions; Cornu Cervi 45 weight portions; Cordyceps 45 weight portions; Radix Polygoni Multiflori 110 weight portions; Carapax Et Plastrum Testudinis 70 weight portions; Fructus Corni 21 weight portions; Fructus Lycii 200 weight portions; The Radix Astragali 50 weight portions; The Rhizoma Atractylodis Macrocephalae 38 weight portions; Ligamentum cervi 10 weight portions; Semen Cuscutae 160 weight portions; Fructus Ligustri Lucidi 9 weight portions; Herba Ecliptae 22 weight portions; Tremella 47 weight portions; Mytilus 49 weight portions; Radix Bupleuri 4 weight portions; Fructus Aurantii 20 weight portions; Pericarpium Citri Reticulatae 3 weight portions; Lignum Aquilariae Resinatum 3 weight portions; Calculus Bovis 15 weight portions; Radix Scutellariae 6 weight portions; Rhizoma Coptidis 6 weight portions; Cortex Phellodendri 18 weight portions; Arillus Longan 50 weight portions; Rhizoma Acori Graminei 6 weight portions; Radix Polygalae 9 weight portions; Radix Salviae Miltiorrhizae 100 weight portions; Radix Notoginseng 6 weight portions; Fructus Jujubae 35 weight portions; Radix Glycyrrhizae 90 weight portions; Radix Codonopsis 17 weight portions; Fructus Schisandrae Chinensis 27 weight portions; Radix Angelicae Sinensis 19 weight portions; Rhizoma Polygonati Odorati 90 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 20 weight portions; Semen pini koraiensis 80 weight portions; Semen Nelumbinis 40 weight portions; Semen Juglandis 110 weight portions; Herba Epimedii 11 weight portions; Rhizoma Dioscoreae 80 weight portions; Fructus Amomi 5 weight portions; Semen Astragali Complanati 180 weight portions; Fructus Aurantii Immaturus 8 weight portions; Radix Morindae Officinalis 28 weight portions; Air Bladder pseudosciaenae seu Acipenser 20 weight portions; Stichopus japonicus 45 weight portions; Radix Rehmanniae 20 weight portions; Cortex Cinnamomi 15 weight portions; Rhizoma Polygonati 100 weight portions; Fructus Psoraleae 27 weight portions; The Radix Paeoniae Alba 38 weight portions; Fructus Mume 28 weight portions; Semen Platycladi 100 weight portions; Colla Corii Asini 8 weight portions; Terminalia flesh 9 weight portions; Herba Cistanches 40 weight portions; Fructus Litseae 5 weight portions; Poria 85 weight portions.
A kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect, its 59 flavor crude drug composition and weight proportion are preferably following:
Radix Ginseng 8 weight portions; Radix Rehmanniae Preparata 45 weight portions; Cornu Cervi 50 weight portions; Cordyceps 45 weight portions; Radix Polygoni Multiflori 110 weight portions; Carapax Et Plastrum Testudinis 70 weight portions; Fructus Corni 21 weight portions; Fructus Lycii 200 weight portions; The Radix Astragali 50 weight portions; The Rhizoma Atractylodis Macrocephalae 38 weight portions; Ligamentum cervi 10 weight portions; Semen Cuscutae 160 weight portions; Fructus Ligustri Lucidi 9 weight portions; Herba Ecliptae 22 weight portions; Tremella 47 weight portions; Mytilus 55 weight portions; Radix Bupleuri 4 weight portions; Fructus Aurantii 20 weight portions; Pericarpium Citri Reticulatae 3 weight portions; Lignum Aquilariae Resinatum 3 weight portions; Calculus Bovis 15 weight portions; Radix Scutellariae 6 weight portions; Rhizoma Coptidis 6 weight portions; Cortex Phellodendri 18 weight portions; Arillus Longan 50 weight portions; Rhizoma Acori Graminei 6 weight portions; Radix Polygalae 9 weight portions; Radix Salviae Miltiorrhizae 100 weight portions; Radix Notoginseng 6 weight portions; Fructus Jujubae 35 weight portions; Radix Glycyrrhizae 90 weight portions; Radix Codonopsis 17 weight portions; Fructus Schisandrae Chinensis 27 weight portions; Radix Angelicae Sinensis 19 weight portions; Rhizoma Polygonati Odorati 90 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 20 weight portions; Semen pini koraiensis 80 weight portions; Semen Nelumbinis 40 weight portions; Semen Juglandis 110 weight portions; Herba Epimedii 11 weight portions; Rhizoma Dioscoreae 80 weight portions; Fructus Amomi 5 weight portions; Semen Astragali Complanati 180 weight portions; Fructus Aurantii Immaturus 8 weight portions; Radix Morindae Officinalis 28 weight portions; Air Bladder pseudosciaenae seu Acipenser 20 weight portions; Stichopus japonicus 45 weight portions; Radix Rehmanniae 20 weight portions; Cortex Cinnamomi 15 weight portions; Rhizoma Polygonati 100 weight portions; Fructus Psoraleae 27 weight portions; The Radix Paeoniae Alba 38 weight portions; Fructus Mume 28 weight portions; Semen Platycladi 100 weight portions; Colla Corii Asini 9 weight portions; Terminalia flesh 9 weight portions; Herba Cistanches 40 weight portions; Fructus Litseae 5 weight portions; Poria 85 weight portions.
59 flavor crude drug of above-mentioned Chinese medicine composition; Add conventional adjuvant; According to conventional method, process mixture, concentrated pill, capsule, drop pill, granule, tablet, pill, soft capsule, slow releasing agent, oral liquid or the lyophilized injectable powder of clinical acceptance.
The preferred Radix Polygoni Multiflori (processed with Semen sojae atricolor wine) of Radix Polygoni Multiflori in the above-mentioned raw materials medicine, the preferred stir-baking in sand quench in vinegar of Carapax Et Plastrum Testudinis Carapax Et Plastrum Testudinis, the preferred wine Fructus Corni Preparata of Fructus Corni, the preferred Radix Astragali (processed with Mel) of the Radix Astragali; The preferred Rhizoma Atractylodis Macrocephalae (parched with bran) of the Rhizoma Atractylodis Macrocephalae, the preferred Fructus Ligustri Lucidi (processed with wine) of Fructus Ligustri Lucidi, the preferred stir-baked Fructus Aurantii in bran of Fructus Aurantii; The preferred Radix Glycyrrhizae Cortex et Radix Polygalae (processed) of Radix Polygalae, the preferred Radix Glycyrrhizae (processed with honey) of Radix Glycyrrhizae, the preferred processed with vinegar Fructus Schisandrae Chinensis of Fructus Schisandrae Chinensis; The preferred Adeps Caprae seu ovis Herba Epimedii Preparata of Herba Epimedii, the preferred Fructus Aurantii Immaturus (parched) of Fructus Aurantii Immaturus, the preferred Radix Glycyrrhizae Radix Morindae Officinalis (processed) of Radix Morindae Officinalis; The preferred wine of Rhizoma Polygonati is processed Rhizoma Polygonati, Fructus Psoraleae preferred salt Fructus Psoraleae (processed), the preferred wine Herba Cistanches (processed) of Herba Cistanches.Cortex Phellodendri can replace with Cortex Phellodendri.
A kind of method for preparing with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect; Its preparation method is: a kind of method for preparing with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect; Its preparation method is: get crude drug by weight ratio, wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii, be ground into fine powder; Sieve; Mixing with the Calculus Bovis facing-up, sieves; Mixing gets powder; All the other flavour of a drug, decocte with water 2-4 time, each 1-4 hour, filter, merging filtrate is concentrated into the thick paste of 40-90 ℃ of relative density 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Fine powder adds conventional adjuvant, according to conventional method, processes mixture, concentrated pill, capsule, drop pill, granule, tablet, pill, soft capsule, slow releasing agent, oral liquid or the lyophilized injectable powder of clinical acceptance.
A kind of method for preparing with Chinese medicine composition pill of Yin-nourishing and Yang-supporting, element culturing fixed folder effect; Its preparation method is preferably: get crude drug by weight ratio, wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii, be ground into fine powder; Sieve; Mixing with the Calculus Bovis facing-up, sieves; Mixing gets powder; All the other flavour of a drug, decocte with water 2-4 time, each 1-4 hour,, filtering, merging filtrate is concentrated into the thick paste of 40-90 ℃ of relative density 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Every 50-150 weight portion fine powder adds refined honey 15-55 weight portion and suitable quantity of water, pill, and cold drying promptly gets.
A kind of method for preparing with Chinese medicine composition pill of Yin-nourishing and Yang-supporting, element culturing fixed folder effect; Its preparation method is preferably: get crude drug by weight ratio, wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii, be ground into fine powder; Sieve; Mixing with the Calculus Bovis facing-up, sieves; Mixing gets powder; All the other flavour of a drug, decocte with water 2 times, 4 hours for the first time, be respectively 1 hour for the 2nd time, filter, merging filtrate is concentrated into the thick paste of 40 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Per 60 weight portion fine powders add refined honey 20 weight portions and suitable quantity of water, pill, and cold drying promptly gets.
A kind of method for preparing with Chinese medicine composition pill of Yin-nourishing and Yang-supporting, element culturing fixed folder effect; It is preferably: get crude drug by weight ratio, wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii, be ground into fine powder; Sieve; Mixing with the Calculus Bovis facing-up, sieves; Mixing gets powder; All the other flavour of a drug, decocte with water 4 times, the 1st, 2 time 2 hours, be respectively 3 hours for the 3rd, 4 time, filter, merging filtrate is concentrated into the thick paste of 90 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Per 140 weight portion fine powders add refined honey 45 weight portions and suitable quantity of water, pill, and cold drying promptly gets.
A kind of method for preparing with Chinese medicine composition pill of Yin-nourishing and Yang-supporting, element culturing fixed folder effect; It is preferably: get crude drug by weight ratio, wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii, be ground into fine powder; Sieve; Mixing with the Calculus Bovis facing-up, sieves; Mixing gets powder; All the other flavour of a drug, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Per 100 weight portion fine powders add refined honey 35 weight portions and suitable quantity of water, pill, and cold drying promptly gets.
A kind of quality determining method with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect comprises following assay: chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; 5-28: the acetonitrile-water of 60-100 ratio is a mobile phase; The detection wavelength is 320nm; Number of theoretical plate is by 2,3,5, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside calculates should be not less than 3000; The preparation of reference substance solution: precision take by weighing 50-70 ℃ of vacuum drying 3-5 hour 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside reference substance is an amount of, adds methanol and processes the solution that every 1ml contains 5-15 μ g, as reference substance solution; The preparation of need testing solution: get this Chinese medicinal composition preparation under the content uniformity item, pulverize, get 0.5-1.5g, the accurate title, decide; Put in the tool plug conical flask of 50-150mL, the accurate 40-60% methanol 40-60mL that adds claims to decide weight, power 250W; Under the frequency 40KHz condition supersound process 20-40 minute, put coldly, claim again to decide weight, supply weight with 40-60% methanol; Shake up, the 0.45um microporous filter membrane filters, and gets subsequent filtrate, promptly gets; Algoscopy: accurate respectively above-mentioned reference substance solution and each 5-15 μ L of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get; The every 1g of this Chinese medicinal composition preparation contains Radix Polygoni Multiflori with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside, i.e. C
20H
22O
9Meter is no less than 0.08mg.
A kind of quality determining method with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect preferably includes following assay: chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; The acetonitrile-water of 5: 95 ratios is a mobile phase; The detection wavelength is 320nm; Number of theoretical plate is by 2,3,5, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside calculates should be not less than 3000; The preparation of reference substance solution: precision takes by weighing at 2,3,5 of 60 ℃ of vacuum dryings 4 hours, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside reference substance is an amount of, adds methanol and processes the solution that every 1ml contains 6 μ g, as reference substance solution; The preparation of need testing solution: get this Chinese medicinal composition preparation under the content uniformity item, pulverize, get 1g, the accurate title, decide; Put in the tool plug conical flask of 100mL, the accurate 50% methanol 50mL that adds claims to decide weight, power 250W; Supersound process is 30 minutes under the frequency 40KHz condition, puts coldly, claims to decide weight again, supplies weight with 50% methanol; Shake up, the 0.45um microporous filter membrane filters, and gets subsequent filtrate, promptly gets; Algoscopy: accurate respectively above-mentioned reference substance solution and each 10 μ L of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get; The every 1g of this Chinese medicinal composition preparation contains Radix Polygoni Multiflori with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside, i.e. C
20H
22O
9Meter is no less than 0.08mg.
A kind of quality determining method with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect preferably includes following assay: chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; The acetonitrile-water of 25: 75 ratios is a mobile phase; The detection wavelength is 320nm; Number of theoretical plate is by 2,3,5, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside calculates should be not less than 3000; The preparation of reference substance solution: precision takes by weighing at 2,3,5 of 60 ℃ of vacuum dryings 4 hours, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside reference substance is an amount of, adds methanol and processes the solution that every 1ml contains 12 μ g, as reference substance solution; The preparation of need testing solution: get this Chinese medicinal composition preparation under the content uniformity item, pulverize, get 1g, the accurate title, decide; Put in the tool plug conical flask of 100mL, the accurate 50% methanol 50mL that adds claims to decide weight, power 250W; Supersound process is 30 minutes under the frequency 40KHz condition, puts coldly, claims to decide weight again, supplies weight with 50% methanol; Shake up, the 0.45um microporous filter membrane filters, and gets subsequent filtrate, promptly gets; Algoscopy: accurate respectively above-mentioned reference substance solution and each 10 μ L of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get; The every 1g of this Chinese medicinal composition preparation contains Radix Polygoni Multiflori with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside, i.e. C
20H
22O
9Meter is no less than 0.08mg.
A kind of quality determining method with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect preferably includes following assay: chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; The acetonitrile-water of 18: 82 ratios is a mobile phase; The detection wavelength is 320nm; Number of theoretical plate is by 2,3,5, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside calculates should be not less than 3000; The preparation of reference substance solution: precision takes by weighing at 2,3,5 of 60 ℃ of vacuum dryings 4 hours, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside reference substance is an amount of, adds methanol and processes the solution that every 1ml contains 10 μ g, as reference substance solution; The preparation of need testing solution: get this Chinese medicinal composition preparation under the content uniformity item, pulverize, get 1g, the accurate title, decide; Put in the tool plug conical flask of 100mL, the accurate 50% methanol 50mL that adds claims to decide weight, power 250W; Supersound process is 30 minutes under the frequency 40KHz condition, puts coldly, claims to decide weight again, supplies weight with 50% methanol; Shake up, the 0.45um microporous filter membrane filters, and gets subsequent filtrate, promptly gets; Algoscopy: accurate respectively above-mentioned reference substance solution and each 10 μ L of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get; The every 1g of this Chinese medicinal composition preparation contains Radix Polygoni Multiflori with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside, i.e. C
20H
22O
9Meter is no less than 0.08mg.
A kind of quality determining method with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect on the basis of above-mentioned assay, also comprises in the following discrimination method one or more:
Differentiate (1): get this Chinese medicinal composition preparation, put microscopically and observe: the square or type circle property of stone cell class, wall is poor on one side.It is fallow to plant skin epidermis stone cell, and polygonal property, wall thickness are seen in the surface, and the hole ditch is superfine close, and cell includes brown thing.Fiber bunchy or diffusing leaving, wall thickness, surface are seen has longitudinal crack, two ends to be cleaved into palpus shape or more truncate.Plant skin palisade cells 2 row, light line is arranged.Irregular branch agglomerate is colourless, meets chloral hydrate liquid and gradually dissolves.The starch grain simple grain, triangular shape is avette or square is circular, diameter 8-35 μ m, omphalion short seam shape or herringbone shape, visible laminated striation.Endotesta prothenchyma (of wood) brownish red, a type polygonal property is seen on the surface, and wall thickness, chamber are little, include siliceous.The stone cell foresythia, wall thickness, the hole ditch is obvious, and laminated striation is obvious.Fiber is faint yellow, fusiformis, and wall thickness, the hole ditch is thin.
Differentiate (2): get this Chinese medicinal composition preparation 20g, porphyrize, the 150ml that adds diethyl ether, ultrasonic 30 minutes, put coldly, filter, medicinal residues are subsequent use, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Get Fructus Psoraleae control medicinal material 1g, with the method preparation, as control medicinal material solution; According to the thin layer chromatography test, draw each 2～4 μ L of control medicinal material solution and need testing solution, put respectively on same silica gel G thin layer precoated plate; With 2-6: the normal hexane-ethyl acetate of 0.5-1.5 ratio is developing solvent; Launch, take out, dry; Spray is put under the ultra-violet lamp 365nm and is inspected with 10% potassium hydroxide methanol solution; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show two identical blue and white fluorescence speckles;
Differentiate (3): get the medicinal residues under discriminating (2) item, add water-saturated n-butanol 150ml, ultrasonic 30 minutes, put cold; Filter, the filtrating evaporate to dryness, residue adds ethanol 5ml makes dissolving, adds that an amount of aluminium oxide is mixed thoroughly in water-bath, drying; 100～200 orders that fill in advance of packing into, 2g is on the neutral alumina pillar of internal diameter 10mm, with methanol 150ml eluting; Collect eluent, evaporate to dryness, residue add methanol 2ml dissolving, as need testing solution; Get the peoniflorin reference substance, add ethanol and process the solution that 1ml contains 1mg, as reference substance solution; According to the thin layer chromatography test, draw each 20 μ L of above-mentioned two kinds of solution, put respectively on same silica gel G thin layer precoated plate; With 20-40: 1-10: 5-15: the chloroform-ethyl acetate of 0.1-0.3 ratio-methanol-formic acid is developing solvent, launches, and takes out; Dry; Spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing, puts under the daylight and inspects; In the test sample chromatograph, with the corresponding position of reference substance on, show the speckle of same color.
A kind of quality determining method with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect on the basis of above-mentioned assay, preferably also comprises in the following discrimination method one or more:
Differentiate (1): get this Chinese medicinal composition preparation, put microscopically and observe: the square or type circle property of stone cell class, wall is poor on one side.It is fallow to plant skin epidermis stone cell, and polygonal property, wall thickness are seen in the surface, and the hole ditch is superfine close, and cell includes brown thing.Fiber bunchy or diffusing leaving, wall thickness, surface are seen has longitudinal crack, two ends to be cleaved into palpus shape or more truncate.Plant skin palisade cells 2 row, light line is arranged.Irregular branch agglomerate is colourless, meets chloral hydrate liquid and gradually dissolves.The starch grain simple grain, triangular shape is avette or square is circular, diameter 8-35 μ m, omphalion short seam shape or herringbone shape, visible laminated striation.Endotesta prothenchyma (of wood) brownish red, a type polygonal property is seen on the surface, and wall thickness, chamber are little, include siliceous.The stone cell foresythia, wall thickness, the hole ditch is obvious, and laminated striation is obvious.Fiber is faint yellow, fusiformis, and wall thickness, the hole ditch is thin.
Differentiate (2): get this Chinese medicinal composition preparation 20g, porphyrize, the 150ml that adds diethyl ether, ultrasonic 30 minutes, put coldly, filter, medicinal residues are subsequent use, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Get Fructus Psoraleae control medicinal material 1g, with the method preparation, as control medicinal material solution; According to the thin layer chromatography test, draw each 2～4 μ L of control medicinal material solution and need testing solution, put respectively on same silica gel G thin layer precoated plate; Normal hexane-ethyl acetate with 4: 1 ratios is developing solvent; Launch, take out, dry; Spray is put under the ultra-violet lamp 365nm and is inspected with 10% potassium hydroxide methanol solution; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show two identical blue and white fluorescence speckles;
Differentiate (3): get the medicinal residues under discriminating (2) item, add water-saturated n-butanol 150ml, ultrasonic 30 minutes, put cold; Filter, the filtrating evaporate to dryness, residue adds ethanol 5ml makes dissolving, adds that an amount of aluminium oxide is mixed thoroughly in water-bath, drying; 100～200 orders that fill in advance of packing into, 2g is on the neutral alumina pillar of internal diameter 10mm, with methanol 150ml eluting; Collect eluent, evaporate to dryness, residue add methanol 2ml dissolving, as need testing solution; Get the peoniflorin reference substance, add ethanol and process the solution that 1ml contains 1mg, as reference substance solution; According to the thin layer chromatography test, draw each 20 μ L of above-mentioned two kinds of solution, put respectively on same silica gel G thin layer precoated plate; With 30: 5: 10: the chloroform-ethyl acetate of 0.2 ratio-methanol-formic acid was developing solvent, launched, and took out; Dry; Spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing, puts under the daylight and inspects; In the test sample chromatograph, with the corresponding position of reference substance on, show the speckle of same color.
Said Chinese medicinal composition preparation is meant the 59 flavor crude drug by the present invention; Add conventional adjuvant; According to conventional method, process mixture, concentrated pill, capsule, drop pill, granule, tablet, pill, soft capsule, slow releasing agent, oral liquid or the lyophilized injectable powder of clinical acceptance.Cortex Phellodendri according to the invention can replace with Cortex Phellodendri.
Traditional Chinese medicinal composition raw materials of the present invention, (1) monarch drug group: Radix Ginseng, Radix Rehmanniae Preparata, Cornu Cervi, Cordyceps, Radix Polygoni Multiflori; Radix Ginseng function strongly invigorating primordial QI for controlling the key medicine of lung spleen ten thousand void, is applicable to lusterless complexion, severe palpitation, the diseases such as lazy speech, spiritlessness and weakness of breathing hard; Radix Rehmanniae Preparata is nourishing YIN and benefiting blood not only, can mend marrow by spermatogenesis again, and the key medicine for unit, beneficial essence under the training benefit consolidate is usually used in deficiency of kidney yin, and soreness of the waist and knees, dysphoria with feverish sensation in the chest palms and soles, night sweat are quenched one's thirst, deficiency and comsumption of liver-blood, lusterless complexion, diseases such as the vertigo; Cornu Cervi, kidney-replenishing, strengthing aitch bone; Cordyceps both tonifying the lung is cloudy, kidney tonifying sun again, the good merchantable brand for tonifying both YIN and YANG is applicable to deficiency of lung-YIN, moving then do to breathe heavily, sighing frequently, and insufficiency of kidney-YANG see aversion to cold and cold limbs is arranged, frequent micturition or nocturia frequency etc.; Radix Polygoni Multiflori is processed and is used the tonifying liver benefiting essence-blood, doublely can restrain vital essence, slightly warm in nature and not dry, and replenishing vital essence and blood and hold back vital essence is mended and oiliness.Be important drugs in the monarch drug group in the prescription; The monarch drug group is given prominence to Yin-nourishing and Yang-supporting, and negative and positive are two transfers the major function of element culturing fixed folder.(2) ministerial drug group: Carapax Et Plastrum Testudinis, Fructus Corni, Fructus Lycii are strengthened monarch drug with 5, nourishing the liver and kidney, and the reinforcing the heart of nourishing blood, astringency inducing is the merit of unit down; The Radix Astragali, the Rhizoma Atractylodis Macrocephalae and usefulness are strengthened monarch drug spleen invigorating QI invigorating, and the merit of yang invigorating consolidating superficial resistance is defended table and protected admittedly and be enough to resist the exopathogen invasion, the merit multiplication of element culturing fixed folder; Ligamentum cervi, Semen Cuscutae are strengthened supporing yang, the controlling nocturnal emission with astringent drugs of monarch drug, the effect of invigorating the liver and kidney, strengthening bone and muscle jointly; Fructus Ligustri Lucidi, Herba Ecliptae are share and are ERZHI WAN, strengthen the monarch drug invigorating the liver and kidney, and the merit of tonifying YIN blood is applicable to the hepatic and renal YIN deficiency, vertigo and tinnitus, insomnia and dreamful sleep, diseases such as soreness of the waist and knees.(3) adjuvant drug group: Tremella, Mytilus are the shared article of medicine food, can hold up the invigorating the liver and kidney of assistant monarch drug, benefiting essence-blood, and nourishing YIN and moistening the lung is with the fraud of harm temperature compensation impairment of YIN; Radix Bupleuri, Fructus Aurantii, Lignum Aquilariae Resinatum, Pericarpium Citri Reticulatae share, and hold up assistant monarch drug depressed liver-energy dispersing and QI regulating, normalizing the stomach by guiding QI downward, and the chest stuffiness relieving relieving distension, in the tonification article, assistant is stopped up the fraud that stagnates with the article of activating qi for lowering adverse qi with the harm benefit; Radix Scutellariae, Rhizoma Coptidis, Cortex Phellodendri are cold and cool generation with the fire of 5 using corrigent monarchs drug with the clear part of the body cavity above the diaphragm housing the heart and lungs, middle Jiao, part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels noxious dampness together with Calculus Bovis, together help the fraud of fire with the harm temperature compensation; Arillus Longan, SHIGEPU, Radix Polygalae are held up the assistant monarch drug benefit motive, the blood that nourishes heart, tranquilizing the mind, restoring normal coordination between the heart and kidney, the refreshment of having one's ideas straightened out with 5; Radix Salviae Miltiorrhizae, Radix Notoginseng are held up the assistant monarch drug removing heat from blood that nourishes blood with 5, remove the tissue regeneration promoting that becomes silted up, promoting blood circulation to remove obstruction in the collateral.And Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, Rhizoma Polygonati Odorati, Pseudobulbus Bletillae (Rhizoma Bletillae), Semen pini koraiensis, Semen Nelumbinis, Semen Juglandis, Herba Epimedii, Rhizoma Dioscoreae, Fructus Amomi, Semen Astragali Complanati, Fructus Aurantii Immaturus, Radix Morindae Officinalis, Air Bladder pseudosciaenae seu Acipenser, Stichopus japonicus, Radix Rehmanniae, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Fructus Mume, Semen Platycladi, Colla Corii Asini, terminalia flesh, Herba Cistanches, Fructus Litseae, Poria are held up assistant monarch drug, ministerial drug jointly.(4) messenger drug group: Fructus Jujubae can the invigorating the spleen and replenishing QI hemopoietic, and Radix Glycyrrhizae can be concocted all medicines again, is messenger drug altogether.
Full side cooperates plays Yin-nourishing and Yang-supporting altogether, and the merit of element culturing fixed folder is used for internal organs dysfunction, and negative and positive of qi and blood all decreases, and seeing has lusterless complexion; Severe palpitation, the lazy speech of breathing hard, spiritlessness and weakness, moving then work is breathed heavily, and likes out long gas; Aversion to cold and cold limbs, forgetful insomnia, dreaminess, dysphoria with feverish sensation in the chest palms and soles, night sweat or spontaneous perspiration; The vertigo, inappetence, loose stool or constipation, menoxenia, frequent micturition or frequent urination at night.We's medicine is many and not mixed and disorderly, can be described as kind nourishing YIN person, and from positive nourishing YIN, kind YANG invigorating person is from cloudy YANG invigorating.The two accent of negative and positive, element culturing fixed folder, the spleen kidney is taken into account, the example of coordinating water and fire tonifying recipes.
Through experiment confirm: pharmaceutical composition proportioning raw materials group of the present invention can make the SDH increased activity better, downward modulation LDH level; Improve syndrome of deficiency of spleen qi rat blood serum gastrin content; Hepatic glycogen content increases and the decline of serum lactate level after making the mice swimming with a load attached to the body.That is to say that effect is all good than matched group aspect anti-ageing, yang invigorating, QI invigorating.Following experiment and embodiment are used to further specify but are not limited to the present invention.
Proportioning raw materials among the corresponding embodiment 1-3 of proportioning raw materials 1-3 difference that uses in the following experimental example.
Experimental example 1 defying age effect experiment
1, material and medicine
Laboratory animal SPF level Wistar rat, male and female half and half, body weight (180 ± 20) g
Medicine SDH testing cassete
The LDH testing cassete
Pharmaceutical composition proportioning raw materials group 1-3 of the present invention
GUIFU DIHUANG WAN
2, method and result
48 of SPF level Wistar rats are divided into 6 groups immediately: blank group, model group, proportioning raw materials group (0.3gkg
-1D
-1), GUIFU DIHUANG WAN group (1.1gkg
-1D
-1), every group each 8.Outside the blank group subcutaneous injection every day equivalent normal saline, all the other respectively organize animal subject cervical region subcutaneous injection D-galactose (0.125g/kg), every day 1 time, 40d continuously.Continuous irrigation stomach treatment 40d.
Each is organized rat and irritates stomach last 1 time, and fasting can't help all killing inspection behind the water 24h.Get and respectively organize centrifuged supernatant after the liver tissues of rats homogenate, the concrete step of measuring the test kit description in strict accordance with SDH, LDH of measuring is carried out.Adopt the SPSS software statistics; Experimental data representes that with
result checks Treatment Analysis with t.
Aging model liver tissues of rats SDH, the active result of influence of LDH are seen table 1-6
Table 1 pair aging model rat liver is organized SDH, the active influence of LDH (n=8,
)
Compare with the blank group: 1) P＜0.05,2) P＜0.01; Compare with model group: 3) P＜0.05, * P＜0.01; The aging model rat liver is organized the active influence of SDH
Each group of table 2 is carried out t check gained P value table respectively with model group
Model group and the modeling of blank control group comparative descriptions success, model group and proportioning raw materials group and positive controls comparative descriptions are significantly improved aspect the SDH activity increasing after with positive drug and proportioning raw materials group.
Table 3 proportioning raw materials group compares with positive group result
Proportioning raw materials 1-3 is superior to positive controls.
Significant difference comparison sheet between 1,2,3 groups of table 4 proportioning raw materials
Proportioning raw materials group 1,2,3 compares the P value each other all greater than 0.05.
The aging model rat liver is organized the influence of LDH level
Each group of table 5 is carried out t check gained P value table respectively with model group
Model group and the modeling of blank control group comparative descriptions success, model group and proportioning raw materials group and positive controls comparative descriptions are significantly improved aspect horizontal reducing LDH after with positive drug and proportioning raw materials group.
Table 6 proportioning raw materials group compares with positive group result
Proportioning raw materials 1-3 is superior to positive controls.
3, conclusion
Pharmaceutical composition proportioning raw materials group 1-3 of the present invention can make the SDH increased activity, and downward modulation LDH level is improved the hepatocyte energy metabolism, strengthens the cell aerobic respiration, makes cell be in the function active state, improves old and feeble rat liver metabolic capacity.
Experimental example 2 yang invigorating effect experiments
1, material and medicine
Laboratory animal SPF level Kunming mouse, male and female half and half, body weight (20 ± 2) g
Medicine LA testing cassete
The hepatic glycogen testing cassete
Pharmaceutical composition proportioning raw materials group 1-3 of the present invention
GUIFU DIHUANG WAN
2, method and result
48 of SPF level kunming mices are divided into 6 groups immediately: blank group, model group, proportioning raw materials group (0.4gkg
- 1D
-1), GUIFU DIHUANG WAN group (1.6gkg
-1D
-1), every group each 8.Outside the blank group subcutaneous injection every day equivalent normal saline, all the other are respectively organized animal subject and adopt prednisone (HC) with 1 intramuscular injection 25mg/kg (being 0.05ml/10g) every day, continuously 7d.Continuous irrigation stomach treatment 14d.Each organizes mice 30min after the last administration, is equivalent to the weight of body constitution amount 10% at afterbody bundle one, and takes out after putting into tank (26 ℃ of water temperatures) the swimming 10min of 36cm * 25cm * 30cm.Pluck the eyeball blood sampling immediately, centrifuging and taking determination of serum serum lactic acid; And accurately take by weighing liver 200mg, through homogenate, hepatic glycogen is measured in centrifugal back.Adopt the SPSS software statistics; Experimental data representes that with
result checks Treatment Analysis with t.
The result that influences to hepatic glycogen and serum lactic acid after the swimming with a load attached to the body of insufficiency of kidney-YANG mice sees table 7-11.
The influence of hepatic glycogen, serum lactic acid content after the table 7 pair insufficiency of kidney-YANG mice swimming with a load attached to the body (n=8,
)
Compare with the blank group: 1) P＜0.05,2) P＜0.01; Compare with model group: 3) P＜0.05, * P＜0.01;
Influence to hepatic glycogen content after the swimming with a load attached to the body of insufficiency of kidney-YANG mice
Each group of table 8 is carried out t check gained P value table respectively with model group
Model group and the modeling of blank control group comparative descriptions success, model group and proportioning raw materials group and positive controls comparative descriptions increase significantly improvement at hepatic glycogen content after with positive drug and proportioning raw materials group.
Table 9 proportioning raw materials group compares with positive group result
Proportioning raw materials 1-3 is superior to positive controls.
Influence to serum lactic acid content after the swimming with a load attached to the body of insufficiency of kidney-YANG mice
Each group of table 10 is carried out t check gained P value table respectively with model group
Model group and the modeling of blank control group comparative descriptions success, model group and proportioning raw materials group and positive controls comparative descriptions are significantly improving aspect the decline of serum lactate level after with positive drug and proportioning raw materials group.
Table 11 proportioning raw materials group compares with positive group result
Proportioning raw materials 1-3 is superior to positive controls.
3, conclusion
Hepatic glycogen content increased and the decline of serum lactate level after pharmaceutical composition proportioning raw materials group 1-3 of the present invention can make the mice swimming with a load attached to the body, and explanation can improve insufficiency of kidney-YANG mouse liver metabolic capacity.Wherein the effect of proportioning raw materials 1-3 is superior to positive controls.
Experimental example 3 QI invigorating effect experiments
1, materials and methods
Laboratory animal: 48 of cleaning level Wistar rats, male and female half and half, body weight (180 ± 20) g.
Medicine: gastrin radioimmunological kit
Pharmaceutical composition proportioning raw materials group 1-3 of the present invention
BUZHONG YIQI WAN
2, method and result
48 of cleaning level Wistar rats are divided into 6 groups: blank group, model group, proportioning raw materials group (0.3gkg immediately
- 1D
-1), BUZHONG YIQI WAN group (1.7gkg
-1D
-1), every group each 8.First breeding observing 3d before the experiment.
Syndrome of deficiency of spleen qi model: 1. only irritate clothes rhubarb preparation liquid, day 1 time with 3ml/ morning every day; 2. every day, bore a heavy burden animal afternoon, and twining weight in rat root of the tail portion be the electric fuse of this rat body weight 10%, puts into the tank of 20 ℃ of depth of water 50cm, water temperature and swim, and exhausts degree of being with power, and promptly the rat nose does not have upstream face 10s, day 1 time; 3. keep on a diet, the morning every day 8:00 feeding, afternoon, 8:00 removed food.Blank group filling in morning every day clothes normal saline, day 1 time, free diet.Be total to 21d.
From experiment 22d, blank group, model group give normal saline and irritate stomach, and the medication group gives each proportioning raw materials group of pharmaceutical composition of the present invention respectively and BUZHONG YIQI WAN is irritated stomach, altogether 21d.Pluck eyeball respectively at 21d after intervening and get blood, put to death.Pluck eyeball and get blood, separation of serum detects serum gastrin.Adopt the SPSS software statistics; Experimental data representes that with
result checks Treatment Analysis with t.
Radix Et Rhizoma Rhei method, swimming power are exhausted method, starvation method three factor composite methods duplicates the result of syndrome of deficiency of spleen qi rat blood serum gastrin influence and sees table 12-14.
The influence of table 12 pair serum gastrin (n=8,
)
Compare with the blank group: 1) P＜0.05,2) P＜0.01;
Compare with model group: 3) P＜0.05, * P＜0.01;
Each group of table 13 is carried out t check gained P value table respectively with model group
Model group and the modeling of blank control group comparative descriptions success, model group and proportioning raw materials group and positive controls comparative descriptions significantly improve at serum gastrin content after with positive drug and proportioning raw materials group.
Table 14 proportioning raw materials group compares with positive group result
Proportioning raw materials 1-3 is superior to positive controls.
3, conclusion
The serum gastrin content of pharmaceutical composition proportioning raw materials group 1-3 of the present invention raises.Explain that pharmaceutical composition proportioning raw materials group 1-3 of the present invention can improve syndrome of deficiency of spleen qi rat blood serum gastrin content.Wherein the effect of proportioning raw materials 1-3 is superior to the effect of positive controls.
Following embodiment all can realize the effect of above-mentioned experimental example.
Embodiment 1
Proportioning raw materials 1:
Radix Ginseng 9g, Radix Rehmanniae Preparata 27g, Cornu Cervi 45g,
Cordyceps 27g, Radix Polygoni Multiflori (black soya bean wine is processed) 207g, Carapax Et Plastrum Testudinis (stir-baking in sand quench in vinegar) 45g,
Fructus Corni (wine is processed) 27g, Fructus Lycii 207g, the Radix Astragali (processed with honey) 63g,
The Rhizoma Atractylodis Macrocephalae (parched with bran) 63g, Ligamentum cervi 2g, Semen Cuscutae 135g,
Fructus Ligustri Lucidi (wine is processed) 9g, Herba Ecliptae 27g, Tremella 27g,
Mytilus 63g, Radix Bupleuri 4g, Fructus Aurantii (parched with bran) 5g,
Pericarpium Citri Reticulatae 4g, Lignum Aquilariae Resinatum 4g, Calculus Bovis 4g,
Radix Scutellariae 5g, Rhizoma Coptidis 5g, Cortex Phellodendri 4.5g,
Arillus Longan 27g, Rhizoma Acori Graminei 5g, Radix Polygalae (Radix Glycyrrhizae is processed) 9g,
Radix Salviae Miltiorrhizae 63g, Radix Notoginseng 5g, Fructus Jujubae 45g,
Radix Glycyrrhizae (processed with honey) 63g, Radix Codonopsis 9g, Fructus Schisandrae Chinensis (processed with vinegar) 9g,
Radix Angelicae Sinensis 27g, Rhizoma Polygonati Odorati 63g, Pseudobulbus Bletillae (Rhizoma Bletillae) 27g,
Semen pini koraiensis 63g, Semen Nelumbinis 63g, Semen Juglandis 63g
Herba Epimedii (Adeps Caprae seu ovis is processed) 9g, Rhizoma Dioscoreae 63g, Fructus Amomi 4g
Semen Astragali Complanati 135g, Fructus Aurantii Immaturus (stir-fry) 5g, Radix Morindae Officinalis (Radix Glycyrrhizae is processed) 9g,
Air Bladder pseudosciaenae seu Acipenser 27g, Stichopus japonicus 27g, Radix Rehmanniae 27g,
Cortex Cinnamomi 5g, Rhizoma Polygonati (wine is processed) 63g, Fructus Psoraleae (salt is processed) 9g,
Radix Paeoniae Alba 63g, Fructus Mume 9g, Semen Platycladi 63g,
Colla Corii Asini 9g, terminalia flesh 9g, Herba Cistanches (wine is processed) 27g
Fructus Litseae 5g, Poria 63g.
Get crude drug by weight ratio; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Every 100g fine powder adds refined honey 35g and suitable quantity of water, pill, and cold drying promptly gets pill.
Embodiment 2
Proportioning raw materials 2:
Radix Ginseng 9g, Radix Rehmanniae Preparata 36g, Cornu Cervi 54g,
Cordyceps 36g, Radix Polygoni Multiflori (black soya bean wine is processed) 216g, Carapax Et Plastrum Testudinis (stir-baking in sand quench in vinegar) 54g,
Fructus Corni (wine is processed) 36g, Fructus Lycii 216g, the Radix Astragali (processed with honey) 72g,
The Rhizoma Atractylodis Macrocephalae (parched with bran) 72g, Ligamentum cervi 2.25g, Semen Cuscutae 144g,
Fructus Ligustri Lucidi (wine is processed) 18g, Herba Ecliptae 36g, Tremella 36g,
Mytilus 72g, Radix Bupleuri 4.5g, Fructus Aurantii (parched with bran) 9g,
Pericarpium Citri Reticulatae 4.5g, Lignum Aquilariae Resinatum 4.5g, Calculus Bovis 4.5g,
Radix Scutellariae 9g, Rhizoma Coptidis 9g, Cortex Phellodendri 13.5g,
Arillus Longan 36g, Rhizoma Acori Graminei 9g, Radix Polygalae (Radix Glycyrrhizae is processed) 18g,
Radix Salviae Miltiorrhizae 72g, Radix Notoginseng 9g, Fructus Jujubae 54g,
Radix Glycyrrhizae (processed with honey) 72g, Radix Codonopsis 18g, Fructus Schisandrae Chinensis (processed with vinegar) 18g,
Radix Angelicae Sinensis 36g, Rhizoma Polygonati Odorati 72g, Pseudobulbus Bletillae (Rhizoma Bletillae) 36g,
Semen pini koraiensis 72g, Semen Nelumbinis 72g, Semen Juglandis 72g
Herba Epimedii (Adeps Caprae seu ovis is processed) 18g, Rhizoma Dioscoreae 72g, Fructus Amomi 4.5g
Semen Astragali Complanati 144g, Fructus Aurantii Immaturus (stir-fry) 9g, Radix Morindae Officinalis (Radix Glycyrrhizae is processed) 18g,
Air Bladder pseudosciaenae seu Acipenser 36g, Stichopus japonicus 36g, Radix Rehmanniae 36g,
Cortex Cinnamomi 9g, Rhizoma Polygonati (wine is processed) 72g, Fructus Psoraleae (salt is processed) 18g,
Radix Paeoniae Alba 72g, Fructus Mume 18g, Semen Platycladi 72g,
Colla Corii Asini 18g, terminalia flesh 18g, Herba Cistanches (wine is processed) 36g
Fructus Litseae 9g, Poria 72g.
Get crude drug by weight ratio; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Every 100g fine powder adds refined honey 35g and suitable quantity of water, pill, and cold drying promptly gets pill.
Embodiment 3
Proportioning raw materials 3:
Radix Ginseng 9g, Radix Rehmanniae Preparata 45g, Cornu Cervi 63g,
Cordyceps 45g, Radix Polygoni Multiflori (black soya bean wine is processed) 225g, Carapax Et Plastrum Testudinis (stir-baking in sand quench in vinegar) 63g,
Fructus Corni (wine is processed) 45g, Fructus Lycii 225g, the Radix Astragali (processed with honey) 81g,
The Rhizoma Atractylodis Macrocephalae (parched with bran) 81g, Ligamentum cervi 11.25g, Semen Cuscutae 153g,
Fructus Ligustri Lucidi (wine is processed) 27g, Herba Ecliptae 45g, Tremella 45g,
Mytilus 81g, Radix Bupleuri 13.5g, Fructus Aurantii (parched with bran) 18g,
Pericarpium Citri Reticulatae 13.5g, Lignum Aquilariae Resinatum 13.5g, Calculus Bovis 13.5g,
Radix Scutellariae 18g, Rhizoma Coptidis 18g, Cortex Phellodendri 22.5g,
Arillus Longan 45g, Rhizoma Acori Graminei 18g, Radix Polygalae (Radix Glycyrrhizae is processed) 27g,
Radix Salviae Miltiorrhizae 81g, Radix Notoginseng 18g, Fructus Jujubae 63g,
Radix Glycyrrhizae (processed with honey) 81g, Radix Codonopsis 27g, Fructus Schisandrae Chinensis (processed with vinegar) 27g,
Radix Angelicae Sinensis 45g, Rhizoma Polygonati Odorati 81g, Pseudobulbus Bletillae (Rhizoma Bletillae) 45g,
Semen pini koraiensis 81g, Semen Nelumbinis 81g, Semen Juglandis 81g
Herba Epimedii (Adeps Caprae seu ovis is processed) 27g, Rhizoma Dioscoreae 81g, Fructus Amomi 13.5g
Semen Astragali Complanati 153g, Fructus Aurantii Immaturus (stir-fry) 18g, Radix Morindae Officinalis (Radix Glycyrrhizae is processed) 27g,
Air Bladder pseudosciaenae seu Acipenser 45g, Stichopus japonicus 45g, Radix Rehmanniae 45g,
Cortex Cinnamomi 18g, Rhizoma Polygonati (wine is processed) 81g, Fructus Psoraleae (salt is processed) 27g,
Radix Paeoniae Alba 81g, Fructus Mume 27g, Semen Platycladi 81g,
Colla Corii Asini 27g, terminalia flesh 27g, Herba Cistanches (wine is processed) 45g
Fructus Litseae 18g, Poria 81g.
Get crude drug by weight ratio; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Every 100g fine powder adds refined honey 35g and suitable quantity of water, pill, and cold drying promptly gets pill.
Embodiment 4
Radix Ginseng 20g; Radix Rehmanniae Preparata 28g; Cornu Cervi 64g; Cordyceps 26g; Radix Polygoni Multiflori 280g; Carapax Et Plastrum Testudinis 36g; Fructus Corni 47g; Fructus Lycii 145g; Radix Astragali 106g; Rhizoma Atractylodis Macrocephalae 37g; Ligamentum cervi 12g; Semen Cuscutae 105g; Fructus Ligustri Lucidi 34g; Herba Ecliptae 22g; Tremella 46g; Mytilus 49g; Radix Bupleuri 10g; Fructus Aurantii 20g; Pericarpium Citri Reticulatae 8g; Lignum Aquilariae Resinatum 4g; Calculus Bovis 15g; Radix Scutellariae 18g; Rhizoma Coptidis 5g; Cortex Phellodendri 19g; Arillus Longan 26g; Rhizoma Acori Graminei 18g; Radix Polygalae 10g; Radix Salviae Miltiorrhizae 90g; Radix Notoginseng 8g; Fructus Jujubae 60g; Radix Glycyrrhizae 59g; Radix Codonopsis 25g; Fructus Schisandrae Chinensis 10g; Radix Angelicae Sinensis 45g; Rhizoma Polygonati Odorati 39g; Pseudobulbus Bletillae (Rhizoma Bletillae) 45g; Semen pini koraiensis 38g; Semen Nelumbinis 90g; Semen Juglandis 38g; Herba Epimedii 15g; Rhizoma Dioscoreae 90g; Fructus Amomi 10g; Semen Astragali Complanati 180g; Fructus Aurantii Immaturus 6g; Radix Morindae Officinalis 18g; Air Bladder pseudosciaenae seu Acipenser 20g; Stichopus japonicus 47g; Radix Rehmanniae 30g; Cortex Cinnamomi 20g; Rhizoma Polygonati 100g; Fructus Psoraleae 9g; Radix Paeoniae Alba 90g; Fructus Mume 10g; Semen Platycladi 110g; Colla Corii Asini 11g; Terminalia flesh 29g; Herba Cistanches 20g; Fructus Litseae 20g; Poria 55g.
Get crude drug by weight ratio; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Every 100g fine powder adds refined honey 35g and suitable quantity of water, pill, and cold drying promptly gets pill.
Embodiment 5
Radix Ginseng 8g; Radix Rehmanniae Preparata 45g; Cornu Cervi 45g; Cordyceps 45g; Radix Polygoni Multiflori 110g; Carapax Et Plastrum Testudinis 70g; Fructus Corni 21g; Fructus Lycii 200g; Radix Astragali 50g; Rhizoma Atractylodis Macrocephalae 38g; Ligamentum cervi 10g; Semen Cuscutae 160g; Fructus Ligustri Lucidi 9g; Herba Ecliptae 22g; Tremella 47g; Mytilus 49g; Radix Bupleuri 4g; Fructus Aurantii 20g; Pericarpium Citri Reticulatae 3g; Lignum Aquilariae Resinatum 3g; Calculus Bovis 15g; Radix Scutellariae 6g; Rhizoma Coptidis 6g; Cortex Phellodendri 18g; Arillus Longan 50g; Rhizoma Acori Graminei 6g; Radix Polygalae 9g; Radix Salviae Miltiorrhizae 100g; Radix Notoginseng 6g; Fructus Jujubae 35g; Radix Glycyrrhizae 90g; Radix Codonopsis 17g; Fructus Schisandrae Chinensis 27g; Radix Angelicae Sinensis 19g; Rhizoma Polygonati Odorati 90g; Pseudobulbus Bletillae (Rhizoma Bletillae) 20g; Semen pini koraiensis 80g; Semen Nelumbinis 40g; Semen Juglandis 110g; Herba Epimedii 11g; Rhizoma Dioscoreae 80g; Fructus Amomi 5g; Semen Astragali Complanati 180g; Fructus Aurantii Immaturus 8g; Radix Morindae Officinalis 28g; Air Bladder pseudosciaenae seu Acipenser 20g; Stichopus japonicus 45g; Radix Rehmanniae 20g; Cortex Cinnamomi 15g; Rhizoma Polygonati 100g; Fructus Psoraleae 27g; Radix Paeoniae Alba 38g; Fructus Mume 28g; Semen Platycladi 100g; Colla Corii Asini 8g; Terminalia flesh 9g; Herba Cistanches 40g; Fructus Litseae 5g; Poria 85g.Get crude drug by weight ratio; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Every 100g fine powder adds refined honey 35g and suitable quantity of water, pill, and cold drying promptly gets pill.
Embodiment 6
Radix Ginseng 8g; Radix Rehmanniae Preparata 45g; Cornu Cervi 50g; Cordyceps 45g; Radix Polygoni Multiflori 110g; Carapax Et Plastrum Testudinis 70g; Fructus Corni 21g; Fructus Lycii 200g; Radix Astragali 50g; Rhizoma Atractylodis Macrocephalae 38g; Ligamentum cervi 10g; Semen Cuscutae 160g; Fructus Ligustri Lucidi 9g; Herba Ecliptae 22g; Tremella 47g; Mytilus 55g; Radix Bupleuri 4g; Fructus Aurantii 20g; Pericarpium Citri Reticulatae 3g; Lignum Aquilariae Resinatum 3g; Calculus Bovis 15g; Radix Scutellariae 6g; Rhizoma Coptidis 6g; Cortex Phellodendri 18g; Arillus Longan 50g; Rhizoma Acori Graminei 6g; Radix Polygalae 9g; Radix Salviae Miltiorrhizae 100g; Radix Notoginseng 6g; Fructus Jujubae 35g; Radix Glycyrrhizae 90g; Radix Codonopsis 17g; Fructus Schisandrae Chinensis 27g; Radix Angelicae Sinensis 19g; Rhizoma Polygonati Odorati 90g; Pseudobulbus Bletillae (Rhizoma Bletillae) 20g; Semen pini koraiensis 80g; Semen Nelumbinis 40g; Semen Juglandis 110g; Herba Epimedii 11g; Rhizoma Dioscoreae 80g; Fructus Amomi 5g; Semen Astragali Complanati 180g; Fructus Aurantii Immaturus 8g; Radix Morindae Officinalis 28g; Air Bladder pseudosciaenae seu Acipenser 20g; Stichopus japonicus 45g; Radix Rehmanniae 20g; Cortex Cinnamomi 15g; Rhizoma Polygonati 100g; Fructus Psoraleae 27g; Radix Paeoniae Alba 38g; Fructus Mume 28g; Semen Platycladi 100g; Colla Corii Asini 9g; Terminalia flesh 9g; Herba Cistanches 40g; Fructus Litseae 5g; Poria 85g.Get crude drug by weight ratio; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other flavour of a drug, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Add conventional adjuvant,, process the granule of clinical acceptance according to conventional method.
Embodiment 7
Get crude drug by weight proportion among the embodiment 1; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Add conventional adjuvant,, process the soft capsule of clinical acceptance according to conventional method.
Embodiment 8
Get crude drug by weight proportion among the embodiment 6; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Fine powder adds conventional adjuvant, according to conventional method, processes the slow releasing agent of clinical acceptance.
Embodiment 9
Get crude drug by weight proportion among the embodiment 5; Wherein with Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Amount to 33 flavor crude drug and be ground into fine powder, sieve mixing; With the Calculus Bovis facing-up; Sieve, mixing gets powder; All the other 25-components, decocte with water 3 times, 3 hours for the first time, be respectively 2 hours for the 2nd, 3 time, filter, merging filtrate is concentrated into the thick paste of 50 ℃ of relative densities 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Fine powder adds conventional adjuvant, according to conventional method, processes the oral liquid of clinical acceptance.
Embodiment 10
Pill with embodiment 4 preparations, carry out following assay:
Chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; The acetonitrile-water of 5: 95 ratios is a mobile phase; The detection wavelength is 320nm; Number of theoretical plate is by 2,3,5, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside calculates should be not less than 3000; The preparation of reference substance solution: precision takes by weighing at 2,3,5 of 60 ℃ of vacuum dryings 4 hours, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside reference substance is an amount of, adds methanol and processes the solution that every 1ml contains 6 μ g, as reference substance solution; The preparation of need testing solution: get the pill of embodiment 4 preparations under the content uniformity item, pulverize, get 1g, the accurate title, decide; Put in the tool plug conical flask of 100mL, the accurate 50% methanol 50mL that adds claims to decide weight, power 250W; Supersound process is 30 minutes under the frequency 40KHz condition, puts coldly, claims to decide weight again, supplies weight with 50% methanol; Shake up, the 0.45um microporous filter membrane filters, and gets subsequent filtrate, promptly gets; Algoscopy: accurate respectively above-mentioned reference substance solution and each 10 μ L of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get; The every 1g of pill of embodiment 4 preparations contains Radix Polygoni Multiflori with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside, i.e. C
20H
22O
9Meter is no less than 0.08mg.
Embodiment 11
Pill with embodiment 2 preparations, carry out following assay:
Chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; The acetonitrile-water of 18: 82 ratios is a mobile phase; The detection wavelength is 320nm; Number of theoretical plate is by 2,3,5, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside calculates should be not less than 3000; The preparation of reference substance solution: precision takes by weighing at 2,3,5 of 60 ℃ of vacuum dryings 4 hours, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside reference substance is an amount of, adds methanol and processes the solution that every 1ml contains 10 μ g, as reference substance solution; The preparation of need testing solution: get the pill of embodiment 2 preparations under the content uniformity item, pulverize, get 1g, the accurate title, decide; Put in the tool plug conical flask of 100mL, the accurate 50% methanol 50mL that adds claims to decide weight, power 250W; Supersound process is 30 minutes under the frequency 40KHz condition, puts coldly, claims to decide weight again, supplies weight with 50% methanol; Shake up, the 0.45um microporous filter membrane filters, and gets subsequent filtrate, promptly gets; Algoscopy: accurate respectively above-mentioned reference substance solution and each 10 μ L of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get; The every 1g of pill of embodiment 3 preparations contains Radix Polygoni Multiflori with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside, i.e. C
20H
22O
9Meter is no less than 0.08mg.
Embodiment 12
On the basis of embodiment 10, differentiate as follows again:
Get pill, put microscopically and observe: the square or type circle property of stone cell class, wall is poor on one side.It is fallow to plant skin epidermis stone cell, and polygonal property, wall thickness are seen in the surface, and the hole ditch is superfine close, and cell includes brown thing.Fiber bunchy or diffusing leaving, wall thickness, surface are seen has longitudinal crack, two ends to be cleaved into palpus shape or more truncate.Plant skin palisade cells 2 row, light line is arranged.Irregular branch agglomerate is colourless, meets chloral hydrate liquid and gradually dissolves.The starch grain simple grain, triangular shape is avette or square is circular, diameter 8-35 μ m, omphalion short seam shape or herringbone shape, visible laminated striation.Endotesta prothenchyma (of wood) brownish red, a type polygonal property is seen on the surface, and wall thickness, chamber are little, include siliceous.The stone cell foresythia, wall thickness, the hole ditch is obvious, and laminated striation is obvious.Fiber is faint yellow, fusiformis, and wall thickness, the hole ditch is thin.
Embodiment 13
On the basis of embodiment 11, differentiate as follows again:
Get pill 20g, porphyrize, the 150ml that adds diethyl ether, ultrasonic 30 minutes, put coldly, filter, medicinal residues are subsequent use, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Get Fructus Psoraleae control medicinal material 1g, with the method preparation, as control medicinal material solution; According to the thin layer chromatography test, draw each 2～4 μ L of control medicinal material solution and need testing solution, put respectively on same silica gel G thin layer precoated plate; Normal hexane-ethyl acetate with 4: 1 ratios is developing solvent; Launch, take out, dry; Spray is put under the ultra-violet lamp 365nm and is inspected with 10% potassium hydroxide methanol solution; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show two identical blue and white fluorescence speckles.
Embodiment 14
On the basis of embodiment 13, differentiate as follows again:
Get the medicinal residues that embodiment 13 differentiates under the item, add water-saturated n-butanol 150ml, ultrasonic 30 minutes, put cold; Filter, the filtrating evaporate to dryness, residue adds ethanol 5ml makes dissolving, adds that an amount of aluminium oxide is mixed thoroughly in water-bath, drying; 100～200 orders that fill in advance of packing into, 2g is on the neutral alumina pillar of internal diameter 10mm, with methanol 150ml eluting; Collect eluent, evaporate to dryness, residue add methanol 2ml dissolving, as need testing solution; Get the peoniflorin reference substance, add ethanol and process the solution that 1ml contains 1mg, as reference substance solution; According to the thin layer chromatography test, draw each 20 μ L of above-mentioned two kinds of solution, put respectively on same silica gel G thin layer precoated plate; With 30: 5: 10: the chloroform-ethyl acetate of 0.2 ratio-methanol-formic acid was developing solvent, launched, and took out; Dry; Spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing, puts under the daylight and inspects; In the test sample chromatograph, with the corresponding position of reference substance on, show the speckle of same color.
Claims (12)
1. Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect is characterized in that crude drug is formed and weight proportion is following:
Radix Ginseng 7.5-21 weight portion; Radix Rehmanniae Preparata 27-45 weight portion; Cornu Cervi 40-64.8 weight portion; Cordyceps 25-50 weight portion; Radix Polygoni Multiflori 108-288 weight portion; Carapax Et Plastrum Testudinis 36-72 weight portion; Fructus Corni 18-48 weight portion; Fructus Lycii 144-288 weight portion; Radix Astragali 36-108 weight portion; Rhizoma Atractylodis Macrocephalae 36-108 weight portion; Ligamentum cervi 1.875-12 weight portion; Semen Cuscutae 100-168 weight portion; Fructus Ligustri Lucidi 8-35 weight portion; Herba Ecliptae 20-50 weight portion; Tremella 24-48 weight portion; Mytilus 48-96 weight portion; Radix Bupleuri 2-16 weight portion; Fructus Aurantii 4-21 weight portion; Pericarpium Citri Reticulatae 2-16 weight portion; Lignum Aquilariae Resinatum 2-16 weight portion; Calculus Bovis 3-16 weight portion; Radix Scutellariae 4-20 weight portion; Rhizoma Coptidis 4-20 weight portion; Cortex Phellodendri 4-25 weight portion; Arillus Longan 24-54 weight portion; Rhizoma Acori Graminei 4-20 weight portion; Radix Polygalae 8-25 weight portion; Radix Salviae Miltiorrhizae 60-112 weight portion; Radix Notoginseng 4-20 weight portion; Fructus Jujubae 34.2-64.8 weight portion; Radix Glycyrrhizae 57.6-120 weight portion; Radix Codonopsis 8-30 weight portion; Fructus Schisandrae Chinensis 8-30 weight portion; Radix Angelicae Sinensis 18-48 weight portion; Rhizoma Polygonati Odorati 36-96 weight portion; Pseudobulbus Bletillae (Rhizoma Bletillae) 18-48 weight portion; Semen pini koraiensis 36-96 weight portion; Semen Nelumbinis 36-96 weight portion; Semen Juglandis 36-120 weight portion; Herba Epimedii 8-30 weight portion; Rhizoma Dioscoreae 36-96 weight portion; Fructus Amomi 2.25-21 weight portion; Semen Astragali Complanati 72-192 weight portion; Fructus Aurantii Immaturus 4.5-21 weight portion; Radix Morindae Officinalis 8-30 weight portion; Air Bladder pseudosciaenae seu Acipenser 18-48 weight portion; Stichopus japonicus 18-48 weight portion; Radix Rehmanniae 18-48 weight portion; Cortex Cinnamomi 4.5-21 weight portion; Rhizoma Polygonati 36-120 weight portion; Fructus Psoraleae 8-30 weight portion; Radix Paeoniae Alba 36-96 weight portion; Fructus Mume 8-30 weight portion; Semen Platycladi 36-120 weight portion; Colla Corii Asini 8-30 weight portion; Terminalia flesh 8-30 weight portion; Herba Cistanches 18-48 weight portion; Fructus Litseae 4.5-21 weight portion; Poria 50-90 weight portion.
2. a kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect as claimed in claim 1 is characterized in that crude drug is formed and weight proportion is following:
Radix Ginseng 9 weight portions; Radix Rehmanniae Preparata 27 weight portions; Cornu Cervi 45 weight portions; Cordyceps 27 weight portions; Radix Polygoni Multiflori 207 weight portions; Carapax Et Plastrum Testudinis 45 weight portions; Fructus Corni 27 weight portions; Fructus Lycii 207 weight portions; The Radix Astragali 63 weight portions; The Rhizoma Atractylodis Macrocephalae 63 weight portions; Ligamentum cervi 2 weight portions; Semen Cuscutae 135 weight portions; Fructus Ligustri Lucidi 9 weight portions; Herba Ecliptae 27 weight portions; Tremella 27 weight portions; Mytilus 63 weight portions; Radix Bupleuri 4 weight portions; Fructus Aurantii 5 weight portions; Pericarpium Citri Reticulatae 4 weight portions; Lignum Aquilariae Resinatum 4 weight portions; Calculus Bovis 4 weight portions; Radix Scutellariae 5 weight portions; Rhizoma Coptidis 5 weight portions; Cortex Phellodendri 4.5 weight portions; Arillus Longan 27 weight portions; Rhizoma Acori Graminei 5 weight portions; Radix Polygalae 9 weight portions; Radix Salviae Miltiorrhizae 63 weight portions; Radix Notoginseng 5 weight portions; Fructus Jujubae 45 weight portions; Radix Glycyrrhizae 63 weight portions; Radix Codonopsis 9 weight portions; Fructus Schisandrae Chinensis 9 weight portions; Radix Angelicae Sinensis 27 weight portions; Rhizoma Polygonati Odorati 63 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 27 weight portions; Semen pini koraiensis 63 weight portions; Semen Nelumbinis 63 weight portions; Semen Juglandis 63 weight portions; Herba Epimedii 9 weight portions; Rhizoma Dioscoreae 63 weight portions; Fructus Amomi 4 weight portions; Semen Astragali Complanati 135 weight portions; Fructus Aurantii Immaturus 5 weight portions; Radix Morindae Officinalis 9 weight portions; Air Bladder pseudosciaenae seu Acipenser 27 weight portions; Stichopus japonicus 27 weight portions; Radix Rehmanniae 27 weight portions; Cortex Cinnamomi 5 weight portions; Rhizoma Polygonati 63 weight portions; Fructus Psoraleae 9 weight portions; The Radix Paeoniae Alba 63 weight portions; Fructus Mume 9 weight portions; Semen Platycladi 63 weight portions; Colla Corii Asini 9 weight portions; Terminalia flesh 9 weight portions; Herba Cistanches 27 weight portions; Fructus Litseae 5 weight portions; Poria 63 weight portions.
3. a kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect as claimed in claim 1 is characterized in that crude drug is formed and weight proportion is following:
Radix Ginseng 9 weight portions; Radix Rehmanniae Preparata 36 weight portions; Cornu Cervi 54 weight portions; Cordyceps 36 weight portions; Radix Polygoni Multiflori 216 weight portions; Carapax Et Plastrum Testudinis 54 weight portions; Fructus Corni 36 weight portions; Fructus Lycii 216 weight portions; The Radix Astragali 72 weight portions; The Rhizoma Atractylodis Macrocephalae 72 weight portions; Ligamentum cervi 2.25 weight portions; Semen Cuscutae 144 weight portions; Fructus Ligustri Lucidi 18 weight portions; Herba Ecliptae 36 weight portions; Tremella 36 weight portions; Mytilus 72 weight portions; Radix Bupleuri 4.5 weight portions; Fructus Aurantii 9 weight portions; Pericarpium Citri Reticulatae 4.5 weight portions; Lignum Aquilariae Resinatum 4.5 weight portions; Calculus Bovis 4.5 weight portions; Radix Scutellariae 9 weight portions; Rhizoma Coptidis 9 weight portions; Cortex Phellodendri 13.5 weight portions; Arillus Longan 36 weight portions; Rhizoma Acori Graminei 9 weight portions; Radix Polygalae 18 weight portions; Radix Salviae Miltiorrhizae 72 weight portions; Radix Notoginseng 9 weight portions; Fructus Jujubae 54 weight portions; Radix Glycyrrhizae 72 weight portions; Radix Codonopsis 18 weight portions; Fructus Schisandrae Chinensis 18 weight portions; Radix Angelicae Sinensis 36 weight portions; Rhizoma Polygonati Odorati 72 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 36 weight portions; Semen pini koraiensis 72 weight portions; Semen Nelumbinis 72 weight portions; Semen Juglandis 72 weight portions; Herba Epimedii 18 weight portions; Rhizoma Dioscoreae 72 weight portions; Fructus Amomi 4.5 weight portions; Semen Astragali Complanati 144 weight portions; Fructus Aurantii Immaturus 9 weight portions; Radix Morindae Officinalis 18 weight portions; Air Bladder pseudosciaenae seu Acipenser 36 weight portions; Stichopus japonicus 36 weight portions; Radix Rehmanniae 36 weight portions; Cortex Cinnamomi 9 weight portions; Rhizoma Polygonati 72 weight portions; Fructus Psoraleae 18 weight portions; The Radix Paeoniae Alba 72 weight portions; Fructus Mume 18 weight portions; Semen Platycladi 72 weight portions; Colla Corii Asini 18 weight portions; Terminalia flesh 18 weight portions; Herba Cistanches 36 weight portions; Fructus Litseae 9 weight portions; Poria 72 weight portions;
Or
Radix Ginseng 9 weight portions; Radix Rehmanniae Preparata 45 weight portions; Cornu Cervi 63 weight portions; Cordyceps 45 weight portions; Radix Polygoni Multiflori 225 weight portions; Carapax Et Plastrum Testudinis 63 weight portions; Fructus Corni 45 weight portions; Fructus Lycii 225 weight portions; The Radix Astragali 81 weight portions; The Rhizoma Atractylodis Macrocephalae 81 weight portions; Ligamentum cervi 11.25 weight portions; Semen Cuscutae 153 weight portions; Fructus Ligustri Lucidi 27 weight portions; Herba Ecliptae 45 weight portions; Tremella 45 weight portions; Mytilus 81 weight portions; Radix Bupleuri 13.5 weight portions; Fructus Aurantii 18 weight portions; Pericarpium Citri Reticulatae 13.5 weight portions; Lignum Aquilariae Resinatum 13.5 weight portions; Calculus Bovis 13.5 weight portions; Radix Scutellariae 18 weight portions; Rhizoma Coptidis 18 weight portions; Cortex Phellodendri 22.5 weight portions; Arillus Longan 45 weight portions; Rhizoma Acori Graminei 18 weight portions; Radix Polygalae 27 weight portions; Radix Salviae Miltiorrhizae 81 weight portions; Radix Notoginseng 18 weight portions; Fructus Jujubae 63 weight portions; Radix Glycyrrhizae 81 weight portions; Radix Codonopsis 27 weight portions; Fructus Schisandrae Chinensis 27 weight portions; Radix Angelicae Sinensis 45 weight portions; Rhizoma Polygonati Odorati 81 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 45 weight portions; Semen pini koraiensis 81 weight portions; Semen Nelumbinis 81 weight portions; Semen Juglandis 81 weight portions; Herba Epimedii 27 weight portions; Rhizoma Dioscoreae 81 weight portions; Fructus Amomi 13.5 weight portions; Semen Astragali Complanati 153 weight portions; Fructus Aurantii Immaturus 18 weight portions; Radix Morindae Officinalis 27 weight portions; Air Bladder pseudosciaenae seu Acipenser 45 weight portions; Stichopus japonicus 45 weight portions; Radix Rehmanniae 45 weight portions; Cortex Cinnamomi 18 weight portions; Rhizoma Polygonati 81 weight portions; Fructus Psoraleae 27 weight portions; The Radix Paeoniae Alba 81 weight portions; Fructus Mume 27 weight portions; Semen Platycladi 81 weight portions; Colla Corii Asini 27 weight portions; Terminalia flesh 27 weight portions; Herba Cistanches 45 weight portions; Fructus Litseae 18 weight portions; Poria 81 weight portions.
4. a kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect as claimed in claim 1 is characterized in that crude drug is formed and weight proportion is following:
Radix Ginseng 20 weight portions; Radix Rehmanniae Preparata 28 weight portions; Cornu Cervi 64 weight portions; Cordyceps 26 weight portions; Radix Polygoni Multiflori 280 weight portions; Carapax Et Plastrum Testudinis 36 weight portions; Fructus Corni 47 weight portions; Fructus Lycii 145 weight portions; The Radix Astragali 106 weight portions; The Rhizoma Atractylodis Macrocephalae 37 weight portions; Ligamentum cervi 12 weight portions; Semen Cuscutae 105 weight portions; Fructus Ligustri Lucidi 34 weight portions; Herba Ecliptae 22 weight portions; Tremella 46 weight portions; Mytilus 49 weight portions; Radix Bupleuri 10 weight portions; Fructus Aurantii 20 weight portions; Pericarpium Citri Reticulatae 8 weight portions; Lignum Aquilariae Resinatum 4 weight portions; Calculus Bovis 15 weight portions; Radix Scutellariae 18 weight portions; Rhizoma Coptidis 5 weight portions; Cortex Phellodendri 19 weight portions; Arillus Longan 26 weight portions; Rhizoma Acori Graminei 18 weight portions; Radix Polygalae 10 weight portions; Radix Salviae Miltiorrhizae 90 weight portions; Radix Notoginseng 8 weight portions; Fructus Jujubae 60 weight portions; Radix Glycyrrhizae 59 weight portions; Radix Codonopsis 25 weight portions; Fructus Schisandrae Chinensis 10 weight portions; Radix Angelicae Sinensis 45 weight portions; Rhizoma Polygonati Odorati 39 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 45 weight portions; Semen pini koraiensis 38 weight portions; Semen Nelumbinis 90 weight portions; Semen Juglandis 38 weight portions; Herba Epimedii 15 weight portions; Rhizoma Dioscoreae 90 weight portions; Fructus Amomi 10 weight portions; Semen Astragali Complanati 180 weight portions; Fructus Aurantii Immaturus 6 weight portions; Radix Morindae Officinalis 18 weight portions; Air Bladder pseudosciaenae seu Acipenser 20 weight portions; Stichopus japonicus 47 weight portions; Radix Rehmanniae 30 weight portions; Cortex Cinnamomi 20 weight portions; Rhizoma Polygonati 100 weight portions; Fructus Psoraleae 9 weight portions; The Radix Paeoniae Alba 90 weight portions; Fructus Mume 10 weight portions; Semen Platycladi 110 weight portions; Colla Corii Asini 11 weight portions; Terminalia flesh 29 weight portions; Herba Cistanches 20 weight portions; Fructus Litseae 20 weight portions; Poria 55 weight portions;
Or Radix Ginseng 8 weight portions; Radix Rehmanniae Preparata 45 weight portions; Cornu Cervi 45 weight portions; Cordyceps 45 weight portions; Radix Polygoni Multiflori 110 weight portions; Carapax Et Plastrum Testudinis 70 weight portions; Fructus Corni 21 weight portions; Fructus Lycii 200 weight portions; The Radix Astragali 50 weight portions; The Rhizoma Atractylodis Macrocephalae 38 weight portions; Ligamentum cervi 10 weight portions; Semen Cuscutae 160 weight portions; Fructus Ligustri Lucidi 9 weight portions; Herba Ecliptae 22 weight portions; Tremella 47 weight portions; Mytilus 49 weight portions; Radix Bupleuri 4 weight portions; Fructus Aurantii 20 weight portions; Pericarpium Citri Reticulatae 3 weight portions; Lignum Aquilariae Resinatum 3 weight portions; Calculus Bovis 15 weight portions; Radix Scutellariae 6 weight portions; Rhizoma Coptidis 6 weight portions; Cortex Phellodendri 18 weight portions; Arillus Longan 50 weight portions; Rhizoma Acori Graminei 6 weight portions; Radix Polygalae 9 weight portions; Radix Salviae Miltiorrhizae 100 weight portions; Radix Notoginseng 6 weight portions; Fructus Jujubae 35 weight portions; Radix Glycyrrhizae 90 weight portions; Radix Codonopsis 17 weight portions; Fructus Schisandrae Chinensis 27 weight portions; Radix Angelicae Sinensis 19 weight portions; Rhizoma Polygonati Odorati 90 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 20 weight portions; Semen pini koraiensis 80 weight portions; Semen Nelumbinis 40 weight portions; Semen Juglandis 110 weight portions; Herba Epimedii 11 weight portions; Rhizoma Dioscoreae 80 weight portions; Fructus Amomi 5 weight portions; Semen Astragali Complanati 180 weight portions; Fructus Aurantii Immaturus 8 weight portions; Radix Morindae Officinalis 28 weight portions; Air Bladder pseudosciaenae seu Acipenser 20 weight portions; Stichopus japonicus 45 weight portions; Radix Rehmanniae 20 weight portions; Cortex Cinnamomi 15 weight portions; Rhizoma Polygonati 100 weight portions; Fructus Psoraleae 27 weight portions; The Radix Paeoniae Alba 38 weight portions; Fructus Mume 28 weight portions; Semen Platycladi 100 weight portions; Colla Corii Asini 8 weight portions; Terminalia flesh 9 weight portions; Herba Cistanches 40 weight portions; Fructus Litseae 5 weight portions; Poria 85 weight portions.
Or Radix Ginseng 8 weight portions; Radix Rehmanniae Preparata 45 weight portions; Cornu Cervi 50 weight portions; Cordyceps 45 weight portions; Radix Polygoni Multiflori 110 weight portions; Carapax Et Plastrum Testudinis 70 weight portions; Fructus Corni 21 weight portions; Fructus Lycii 200 weight portions; The Radix Astragali 50 weight portions; The Rhizoma Atractylodis Macrocephalae 38 weight portions; Ligamentum cervi 10 weight portions; Semen Cuscutae 160 weight portions; Fructus Ligustri Lucidi 9 weight portions; Herba Ecliptae 22 weight portions; Tremella 47 weight portions; Mytilus 55 weight portions; Radix Bupleuri 4 weight portions; Fructus Aurantii 20 weight portions; Pericarpium Citri Reticulatae 3 weight portions; Lignum Aquilariae Resinatum 3 weight portions; Calculus Bovis 15 weight portions; Radix Scutellariae 6 weight portions; Rhizoma Coptidis 6 weight portions; Cortex Phellodendri 18 weight portions; Arillus Longan 50 weight portions; Rhizoma Acori Graminei 6 weight portions; Radix Polygalae 9 weight portions; Radix Salviae Miltiorrhizae 100 weight portions; Radix Notoginseng 6 weight portions; Fructus Jujubae 35 weight portions; Radix Glycyrrhizae 90 weight portions; Radix Codonopsis 17 weight portions; Fructus Schisandrae Chinensis 27 weight portions; Radix Angelicae Sinensis 19 weight portions; Rhizoma Polygonati Odorati 90 weight portions; Pseudobulbus Bletillae (Rhizoma Bletillae) 20 weight portions; Semen pini koraiensis 80 weight portions; Semen Nelumbinis 40 weight portions; Semen Juglandis 110 weight portions; Herba Epimedii 11 weight portions; Rhizoma Dioscoreae 80 weight portions; Fructus Amomi 5 weight portions; Semen Astragali Complanati 180 weight portions; Fructus Aurantii Immaturus 8 weight portions; Radix Morindae Officinalis 28 weight portions; Air Bladder pseudosciaenae seu Acipenser 20 weight portions; Stichopus japonicus 45 weight portions; Radix Rehmanniae 20 weight portions; Cortex Cinnamomi 15 weight portions; Rhizoma Polygonati 100 weight portions; Fructus Psoraleae 27 weight portions; The Radix Paeoniae Alba 38 weight portions; Fructus Mume 28 weight portions; Semen Platycladi 100 weight portions; Colla Corii Asini 9 weight portions; Terminalia flesh 9 weight portions; Herba Cistanches 40 weight portions; Fructus Litseae 5 weight portions; Poria 85 weight portions.
5. like the arbitrary described a kind of Chinese medicine composition of claim 1-4 with Yin-nourishing and Yang-supporting, element culturing fixed folder effect; It is characterized in that crude drug is added conventional adjuvant; According to conventional method, process capsule, granule, tablet, pill, slow releasing agent, oral liquid or the lyophilized injectable powder of clinical acceptance.
6. like the arbitrary described a kind of Chinese medicine composition of claim 1-5, it is characterized in that the preferred Radix Polygoni Multiflori (processed with Semen sojae atricolor wine) of Radix Polygoni Multiflori in the crude drug, the preferred stir-baking in sand quench in vinegar of Carapax Et Plastrum Testudinis Carapax Et Plastrum Testudinis with Yin-nourishing and Yang-supporting, element culturing fixed folder effect; The preferred wine Fructus Corni Preparata of Fructus Corni, the preferred Radix Astragali (processed with Mel) of the Radix Astragali, the preferred Rhizoma Atractylodis Macrocephalae (parched with bran) of the Rhizoma Atractylodis Macrocephalae; The preferred Fructus Ligustri Lucidi (processed with wine) of Fructus Ligustri Lucidi, the preferred stir-baked Fructus Aurantii in bran of Fructus Aurantii, the preferred Radix Glycyrrhizae Cortex et Radix Polygalae (processed) of Radix Polygalae; The preferred Radix Glycyrrhizae (processed with honey) of Radix Glycyrrhizae, the preferred processed with vinegar Fructus Schisandrae Chinensis of Fructus Schisandrae Chinensis, the preferred Adeps Caprae seu ovis Herba Epimedii Preparata of Herba Epimedii; The preferred Fructus Aurantii Immaturus (parched) of Fructus Aurantii Immaturus, the preferred Radix Glycyrrhizae Radix Morindae Officinalis (processed) of Radix Morindae Officinalis, the preferred wine of Rhizoma Polygonati is processed Rhizoma Polygonati; Fructus Psoraleae preferred salt Fructus Psoraleae (processed), the preferred wine Herba Cistanches (processed) of Herba Cistanches; Cortex Phellodendri can replace with Cortex Phellodendri.
7. a kind of method for preparing with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect as claimed in claim 6 is characterized in that this method is:
Get crude drug by weight ratio, wherein, be ground into fine powder Radix Ginseng, Radix Codonopsis, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Pseudobulbus Bletillae (Rhizoma Bletillae), Tremella, Radix Salviae Miltiorrhizae, Lignum Aquilariae Resinatum, Radix Notoginseng, Semen Nelumbinis, Semen Juglandis, Rhizoma Dioscoreae, Fructus Amomi, Rhizoma Coptidis, Radix Scutellariae, Fructus Aurantii Immaturus, Rhizoma Acori Graminei, Stichopus japonicus, Radix Polygoni Multiflori, Cortex Cinnamomi, Rhizoma Polygonati, Fructus Psoraleae, the Radix Paeoniae Alba, Semen Cuscutae, Semen Platycladi, Cordyceps, Colla Corii Asini, Poria, Fructus Litseae, Radix Bupleuri, Fructus Aurantii; Sieve; Mixing with the Calculus Bovis facing-up, sieves; Mixing gets powder; All the other flavour of a drug, decocte with water 2-4 time, each 1-4 hour, filter, merging filtrate is concentrated into the thick paste of 40-90 ℃ of relative density 1.35～1.40; Add above-mentioned powder, mixing, drying is ground into fine powder; Fine powder adds conventional adjuvant, according to conventional method, processes capsule, granule, tablet, pill, slow releasing agent, oral liquid or the lyophilized injectable powder of clinical acceptance.
8. like the arbitrary described a kind of detection method of claim 1-6, it is characterized in that comprising following assay with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect:
Chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; The acetonitrile-water of 5-28:60-100 ratio is a mobile phase; The detection wavelength is 320nm; Number of theoretical plate is by 2,3,5, and 4 '-tetrahydroxystilbene-2-O-β-D-glucoside calculates should be not less than 3000; The preparation of reference substance solution: precision take by weighing 50-70 ℃ of vacuum drying 3-5 hour 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside reference substance is an amount of, adds methanol and processes the solution that every 1ml contains 5-15 μ g, as reference substance solution; The preparation of need testing solution: get this Chinese medicinal composition preparation under the content uniformity item, pulverize, get 0.5-1.5g, the accurate title, decide; Put in the tool plug conical flask of 50-150 mL, the accurate 40-60% methanol 40-60mL that adds claims to decide weight, power 250W; Under the frequency 40KHz condition supersound process 20-40 minute, put coldly, claim again to decide weight, supply weight with 40-60% methanol; Shake up, the 0.45um microporous filter membrane filters, and gets subsequent filtrate, promptly gets; Algoscopy: accurate respectively above-mentioned reference substance solution and each 5-15 μ L of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get; The every 1g of this Chinese medicinal composition preparation contains Radix Polygoni Multiflori with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-glucoside, i.e. C
20H
22O
9Meter is no less than 0.08mg.
9. a kind of detection method with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect as claimed in claim 8 is characterized in that on the basis of above-mentioned assay, also comprises in the following discrimination method one or more:
Differentiate (1): get this Chinese medicinal composition preparation, put microscopic examination: the square or type circle property of stone cell class, wall is poor on one side; It is fallow to plant skin epidermis stone cell, and polygonal property, wall thickness are seen in the surface, and the hole ditch is superfine close, and cell includes brown thing; Fiber bunchy or diffusing leaving, wall thickness, surface are seen has longitudinal crack, two ends to be cleaved into palpus shape or more truncate; Plant skin palisade cells 2 row, light line is arranged; The dendritic agglomerate of irregular branch is colourless, meets chloral hydrate liquid and gradually dissolves; The starch grain simple grain, triangular shape is avette or square is circular, diameter 8-35 μ m, omphalion short seam shape or herringbone shape, visible laminated striation; Endotesta prothenchyma (of wood) brownish red, a type polygonal property is seen on the surface, and wall thickness, chamber are little, include siliceous; The stone cell foresythia, wall thickness, the hole ditch is obvious, and laminated striation is obvious; Fiber is faint yellow, fusiformis, and wall thickness, the hole ditch is thin;
Differentiate (2): get this Chinese medicinal composition preparation 20g, porphyrize, the 150ml that adds diethyl ether, ultrasonic 30 minutes, put coldly, filter, medicinal residues are subsequent use, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Get Fructus Psoraleae control medicinal material 1 g, with the method preparation, as control medicinal material solution; According to the thin layer chromatography test, draw each 2～4 μ L of control medicinal material solution and need testing solution, put respectively on same silica gel G thin layer precoated plate; With 2-6: the normal hexane-ethyl acetate of 0.5-1.5 ratio is developing solvent; Launch, take out, dry; Spray is put under the ultra-violet lamp 365nm and is inspected with 10% potassium hydroxide methanol solution; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show two identical blue and white fluorescence speckles;
Differentiate (3): get the medicinal residues under discriminating (2) item, add water-saturated n-butanol 150ml, ultrasonic 30 minutes, put cold; Filter, the filtrating evaporate to dryness, residue adds ethanol 5ml makes dissolving, adds that an amount of aluminium oxide is mixed thoroughly in water-bath, drying; 100～200 orders that fill in advance of packing into, 2g is on the neutral alumina pillar of internal diameter 10mm, with methanol 150ml eluting; Collect eluent, evaporate to dryness, residue add methanol 2ml dissolving, as need testing solution; Get the peoniflorin reference substance, add ethanol and process the solution that 1ml contains 1mg, as reference substance solution; According to the thin layer chromatography test, draw each 20 μ L of above-mentioned two kinds of solution, put respectively on same silica gel G thin layer precoated plate; With 20-40: 1-10: 5-15: the chloroform-ethyl acetate of 0.1-0.3 ratio-methanol-formic acid is developing solvent, launches, and takes out; Dry; Spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing, puts under the daylight and inspects; In the test sample chromatograph, with the corresponding position of reference substance on, show the speckle of same color.
10. the application in, yang invigorating anti-ageing in preparation, the QI invigorating medicine like the arbitrary described a kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect of claim 1-6.
11. a kind of Chinese medicine composition with Yin-nourishing and Yang-supporting, element culturing fixed folder effect as claimed in claim 5 is characterized in that described capsule is a soft capsule; Pill is concentrated pill or drop pill; Oral liquid is a mixture.
12. a kind of method for preparing with Chinese medicine composition of Yin-nourishing and Yang-supporting, element culturing fixed folder effect as claimed in claim 7 is characterized in that described capsule is a soft capsule; Pill is concentrated pill or drop pill; Oral liquid is a mixture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110324167A CN102363009B (en) | 2011-10-21 | 2011-10-21 | Traditional Chinese medicine composition with yin nourishing, yang assisting, vital essence reinforcing efficacies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110324167A CN102363009B (en) | 2011-10-21 | 2011-10-21 | Traditional Chinese medicine composition with yin nourishing, yang assisting, vital essence reinforcing efficacies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102363009A CN102363009A (en) | 2012-02-29 |
| CN102363009B true CN102363009B (en) | 2012-09-05 |
Family
ID=45689625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110324167A Active CN102363009B (en) | 2011-10-21 | 2011-10-21 | Traditional Chinese medicine composition with yin nourishing, yang assisting, vital essence reinforcing efficacies |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102363009B (en) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102579799B (en) * | 2012-03-06 | 2013-09-25 | 孙雪晶 | Traditional Chinese medicine composition for treating postpartum fever with blood deficiency |
| CN102526541B (en) * | 2012-03-22 | 2013-11-06 | 常熟市虞山绿茶有限公司 | Composition for effectively conditioning female climacteric syndrome |
| CN102657735A (en) * | 2012-04-21 | 2012-09-12 | 申志恩 | Medicament capable of prolonging life by regular intake and production method thereof |
| CN102698238A (en) * | 2012-06-15 | 2012-10-03 | 李承平 | Group of capsules for nourishing kidney and tonifying yang |
| CN102727714B (en) * | 2012-07-03 | 2014-08-20 | 李广裕 | Traditional Chinese medicinal capsule for treating azoospermia |
| CN102772704B (en) * | 2012-08-15 | 2014-08-13 | 殷广全 | Medicament for treating geriatric disease |
| CN102846824B (en) * | 2012-08-28 | 2014-07-02 | 杨高林 | Traditional Chinese medicinal composition for treating cold dampness blood stasis blocking channel type diabetic feet, and its preparation method |
| CN102895521B (en) * | 2012-11-15 | 2013-10-09 | 李凯 | Traditional Chinese medicine for treating ankylosing spondylitis |
| CN102935165B (en) * | 2012-12-10 | 2014-10-08 | 青岛文创科技有限公司 | Traditional Chinese medicinal composition for treating qi deficiency type advanced menstruation |
| CN103893650B (en) * | 2012-12-26 | 2015-03-18 | 刘药石 | Medicament capable of nourishing vigour and strengthening the foundation of immunity |
| CN104248711B (en) * | 2013-06-28 | 2017-06-06 | 张少鹏 | A kind of SHENQUE acupoint health patch |
| CN103386085B (en) * | 2013-07-20 | 2015-04-22 | 陈磊 | Traditional Chinese medicine composition for treating anejaculation |
| CN103341039B (en) * | 2013-07-26 | 2014-11-19 | 甘肃天容堂药业有限公司 | Nourishing formula for enhancing human body immunity and production method of soup thereof |
| CN103417845B (en) * | 2013-08-19 | 2015-05-13 | 丁彬 | Traditional Chinese medicine preparation used for preventing and treating postoperative complications of gastric cancer and preparation method thereof |
| CN103611052A (en) * | 2013-11-28 | 2014-03-05 | 李玉芳 | Medicament for treating deficiency of qi and blood |
| CN103705621B (en) * | 2013-12-10 | 2015-06-10 | 章丘市中医医院 | Formula and preparation method of traditional Chinese medicine for promoting recovery and improving deficiency of qi and blood after orthopedic surgery |
| CN103736019B (en) * | 2013-12-31 | 2016-04-20 | 陈明友 | A kind of Chinese medicine preparation of Cure for insomnia dreaminess |
| CN103725573B (en) * | 2014-01-13 | 2015-08-12 | 江苏海昇药业有限公司 | A kind of imperial osmanthus wine and preparation method thereof |
| CN103705733B (en) * | 2014-01-17 | 2015-09-30 | 陈欣 | One treats ARR Chinese medicinal capsule and preparation method thereof |
| CN103830575A (en) * | 2014-03-03 | 2014-06-04 | 乔蕾 | Foot-bath traditional Chinese medicine for treating constitution of qi deficiency |
| CN103908520A (en) * | 2014-04-12 | 2014-07-09 | 陈欣 | Spleen-invigorating and kidney-nourishing preparation for relieving postoperative radiotherapy reaction of tumor patients and preparation method thereof |
| CN103990101A (en) * | 2014-06-13 | 2014-08-20 | 薛枫 | Traditional Chinese medicine composition for treating gynecological disease |
| CN104126799B (en) * | 2014-07-31 | 2015-12-30 | 桂林融通科技有限公司 | A kind of oral liquid removing wrinkle and preparation method thereof |
| CN104116930A (en) * | 2014-08-18 | 2014-10-29 | 青岛百慧智业生物科技有限公司 | Traditional Chinese medicament for treating liver-kidney yin deficiency type meniere disease and preparation method thereof |
| CN105455087A (en) * | 2014-08-29 | 2016-04-06 | 黄戈 | Dog bone soup making method |
| CN104208554A (en) * | 2014-09-19 | 2014-12-17 | 胡康有 | Traditional Chinese medicine mainly for treating multiple female diseases |
| CN104398792A (en) * | 2014-10-17 | 2015-03-11 | 何兵 | Rhizomyidae drug wine for old people, and preparation method thereof |
| CN104491435B (en) * | 2014-12-12 | 2015-06-10 | 高志芳 | Traditional Chinese Medicine for treating neurose and its preparation method |
| CN104585756B (en) * | 2014-12-31 | 2016-05-04 | 广州至信中药饮片有限公司 | Five ginseng Chinese caterpillar fungus radix polygonati officinalis lotus seeds herbal cuisine and preparation method thereof |
| CN105250864A (en) * | 2015-03-16 | 2016-01-20 | 刘芳 | Traditional Chinese medicine for treating visceral dysfunction |
| CN104719534A (en) * | 2015-03-18 | 2015-06-24 | 苏州市天灵中药饮片有限公司 | Anti-aging tea bag and preparation method thereof |
| CN105230891A (en) * | 2015-11-10 | 2016-01-13 | 安徽金健桥医疗科技有限公司 | Ginseng, orange peel and poria cocos tea beverage and preparation method thereof |
| CN105381358A (en) * | 2015-11-24 | 2016-03-09 | 薛学明 | Medicine for improving immunity and preparation method thereof |
| CN106860787A (en) * | 2017-04-17 | 2017-06-20 | 杨长安 | The Chinese medicine composition of Yin-nourishing and body fluid promoting anti-aging |
| CN107823377A (en) * | 2017-10-25 | 2018-03-23 | 陆惠红 | A kind of Chinese medicine for treating irregular menstruation |
| CN113125569B (en) * | 2019-12-30 | 2022-07-22 | 湖南易能生物医药有限公司 | Fingerprint spectrum determination method and quality control method based on Guyinshi material |
| CN114288355A (en) * | 2021-12-28 | 2022-04-08 | 乌日嘎 | Deficiency-tonifying and life-prolonging traditional Chinese medicine composition, traditional Chinese medicine preparation, preparation method and application thereof |
| EP4335449A1 (en) * | 2022-08-29 | 2024-03-13 | NuLiv Science USA, Inc. | Composition of botanical extracts for sleep and mood support |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1186437C (en) * | 2002-07-17 | 2005-01-26 | 龚文强 | Yellow wine for taking care of health and beautifying face and its preparing process |
-
2011
- 2011-10-21 CN CN201110324167A patent/CN102363009B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1186437C (en) * | 2002-07-17 | 2005-01-26 | 龚文强 | Yellow wine for taking care of health and beautifying face and its preparing process |
Non-Patent Citations (1)
| Title |
|---|
| 高益民.施今墨的经典方药――益肾强身丸、防衰益寿丸.《首都医药》.2005,(第07期),37-53. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102363009A (en) | 2012-02-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102363009B (en) | Traditional Chinese medicine composition with yin nourishing, yang assisting, vital essence reinforcing efficacies | |
| CN102078520B (en) | Chinese medicinal preparation for treating nervous headache and preparation method thereof | |
| CN103041209B (en) | Chinese herbal preparation for curing deficiency of kidney-yin and preparation method thereof | |
| CN102579669B (en) | Chinese medicinal composition for nourishing qi and blood and conditioning menstruation and preparation method thereof | |
| CN100400075C (en) | Qi-invigorating, blood-nourishing medicinal composition and its preparing method | |
| CN112641915A (en) | Traditional Chinese medicine ointment formula for treating palpitation and preparation method thereof | |
| CN106177053A (en) | A kind of Hyperglycemic health care compositions comprising leaf of Cyclocarya paliurus Iljinskaja and Pericarpium Citri Reticulatae | |
| CN106173605A (en) | A kind of Hyperglycemic health care compositions comprising Radix Puerariae and Herba Dendrobii | |
| CN106177477A (en) | A kind of health composition for blood sugar lowering, blood fat reducing and blood pressure lowering | |
| CN103638199B (en) | A kind ofly prevent and treat Chinese medicine composition of neurasthenia and senile dementia and preparation method thereof | |
| CN105796845A (en) | Application of medicine composition in preparing medicine for treating female climacteric syndromes | |
| CN104524292A (en) | Application of traditional Chinese medicine preparation in preparation of medicament for treating obesity | |
| CN106177054A (en) | A kind of Hyperglycemic health care compositions comprising Cortex Mori and Pericarpium Citri Reticulatae | |
| CN105727089A (en) | Application of medicine composition containing folium artemisiae argyi to preparing medicine for treating irritable bowel syndrome | |
| CN106177476A (en) | A kind of Hyperglycemic health care compositions comprising Herba Dendrobii and Pericarpium Citri Reticulatae | |
| CN104547738A (en) | Traditional Chinese medicine preparation for treating obesity and preparation method of traditional Chinese medicine preparation | |
| CN103316106A (en) | Danshan fat reducing granules and preparation method thereof | |
| CN108186821A (en) | A kind of Chinese medicine composition for treating nervous headache and its preparation method and application | |
| CN107638493A (en) | Treat dyspeptic pharmaceutical composition and its production and use | |
| CN100502912C (en) | Medicine for treating deficiency syndrome, its preparing process and usage | |
| CN103041288A (en) | Chinese medicinal composition for treating diabetic fatty liver and preparation process thereof | |
| CN101244127B (en) | Medicament for treating vascular dementia | |
| CN106177480A (en) | A kind of Hyperglycemic health care compositions comprising leaf of Cyclocarya paliurus Iljinskaja and Herba Dendrobii | |
| CN1359702A (en) | Formula and production process of supplement | |
| CN105727237A (en) | Folium artemisiae argyi-containing pharmaceutical composition for treating amenorrhea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240429 Address after: No. 33 Tiangui Street, Daxing Biomedical Industry Base, Zhongguancun Science and Technology Park, Daxing District, Beijing, 100062 Patentee after: BEIJING TONGRENTANG Co.,Ltd. Country or region after: China Address before: Room B1-202, No. 8 West Ring South Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing, 100176 Patentee before: Beijing Tongrentang Natural Pharmaceutical Co.,Ltd. Country or region before: China |
|
| TR01 | Transfer of patent right |