CN102351320B - Method for preparing novel biological microcapsule for biological fluidized bed - Google Patents
Method for preparing novel biological microcapsule for biological fluidized bed Download PDFInfo
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- CN102351320B CN102351320B CN201110183304.2A CN201110183304A CN102351320B CN 102351320 B CN102351320 B CN 102351320B CN 201110183304 A CN201110183304 A CN 201110183304A CN 102351320 B CN102351320 B CN 102351320B
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- sodium alginate
- chitosan
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- 239000003094 microcapsule Substances 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title abstract description 15
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000000661 sodium alginate Substances 0.000 claims abstract description 31
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 31
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 31
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 29
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 229920001661 Chitosan Polymers 0.000 claims abstract description 22
- 238000006731 degradation reaction Methods 0.000 claims abstract description 12
- 230000015556 catabolic process Effects 0.000 claims abstract description 11
- 239000011324 bead Substances 0.000 claims abstract description 8
- 239000000126 substance Substances 0.000 claims abstract description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 6
- 150000001768 cations Chemical class 0.000 claims abstract description 6
- 239000000648 calcium alginate Substances 0.000 claims abstract description 5
- 235000010410 calcium alginate Nutrition 0.000 claims abstract description 5
- 229960002681 calcium alginate Drugs 0.000 claims abstract description 5
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 claims abstract description 5
- 239000001509 sodium citrate Substances 0.000 claims abstract description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 4
- 229920002535 Polyethylene Glycol 1500 Polymers 0.000 claims abstract description 3
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- RFFLAFLAYFXFSW-UHFFFAOYSA-N 1,2-dichlorobenzene Chemical compound ClC1=CC=CC=C1Cl RFFLAFLAYFXFSW-UHFFFAOYSA-N 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 14
- 239000008223 sterile water Substances 0.000 claims description 12
- 230000000593 degrading effect Effects 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 9
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 8
- 229940072056 alginate Drugs 0.000 claims description 8
- 235000010443 alginic acid Nutrition 0.000 claims description 8
- 229920000615 alginic acid Polymers 0.000 claims description 8
- 239000011575 calcium Substances 0.000 claims description 8
- 229910052791 calcium Inorganic materials 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 8
- 229920000053 polysorbate 80 Polymers 0.000 claims description 8
- 239000002351 wastewater Substances 0.000 claims description 7
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 4
- 229910001424 calcium ion Inorganic materials 0.000 claims description 4
- 210000002808 connective tissue Anatomy 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- 239000002504 physiological saline solution Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 150000003384 small molecules Chemical class 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 2
- 150000002500 ions Chemical class 0.000 claims description 2
- 206010011732 Cyst Diseases 0.000 claims 1
- 208000031513 cyst Diseases 0.000 claims 1
- 239000002775 capsule Substances 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 3
- 239000001110 calcium chloride Substances 0.000 abstract 2
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 2
- 239000011248 coating agent Substances 0.000 abstract 2
- 238000000576 coating method Methods 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 150000001450 anions Chemical class 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- WXNZTHHGJRFXKQ-UHFFFAOYSA-N 4-chlorophenol Chemical compound OC1=CC=C(Cl)C=C1 WXNZTHHGJRFXKQ-UHFFFAOYSA-N 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 229940090668 parachlorophenol Drugs 0.000 description 6
- 239000000499 gel Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000005273 aeration Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 239000011824 nuclear material Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229920000867 polyelectrolyte Polymers 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920001131 Pulp (paper) Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for preparing a novel biological microcapsule for a biological fluidized bed. The microcapsule is prepared through treating sodium alginate, powdery active carbon, calcium chloride and chitosan as capsule materials, and a dominant degradation bacterium as a capsule core, preparing calcium alginate gel beads through carrying out ion exchange on sodium alginate and calcium chloride, coating the surface of the gel beads with chitosan, liquefying the gel beads in sodium citrate, and coating the gel beads with sodium alginate. The diameter of the prepared microcapsule is 3.0-4.0mm, the membrane thickness of the prepared microcapsule is 10-20mum, the density of the prepared microcapsule is 1.05-1.08g/mL, and the maximum interception molecular weight of the prepared microcapsule is about PEG 4000, so a micromolecular substance with the relative molecular weight of less than PEG 1500 can freely go through the capsule membrane. Acidic conditions and most divalent cations allow the microcapsule to be stable, and monovalent cations and most anions are bad for the stability of the microcapsule.
Description
One, technical field
The invention belongs to the microbiological treatment technical field of sewage, be specifically related to a kind of preparation method of the novel biology microcapsule that is applied to biological fluidized bed.
Two, background technology
Microencapsulation technology is that enzyme, coenzyme, protein and other or microorganism, animal and plant cells are encapsulated in to the pearl, ellipticity or the ellipsoid shape microcapsule that in the hydrophilic semi-permeable membranes of one deck, form, biomacromolecule and cell are blocked in film or outside film, and allow cell required oxygen, nutritive substance and other small-molecule substances freely to enter and freely the discharging of capsule intracellular metabolite product, thereby reach catalysis, cultivation or immune object of isolating.At present, bio-microcapsule technology all has application in every field such as food and fermentation industries, chemosynthesis industry, environmental pollution improvements.
The method of microencapsulation technology imbedded microbe mainly contains: absorption method, entrapping method and crosslinking, wherein the most frequently used is entrapping method.Entrapping method is that microorganism is embedded in semipermeable polymkeric substance or film, makes microorganism enter porous carrier inside.Be characterized in that operation is simple and feasible, little on microbic activity impact.Conventional embedded material has at present: agar, sodium alginate, chitosan, polyvinyl alcohol, polyacrylamide etc.Wherein two kinds of natural polymers of sodium alginate and chitosan have good biocompatibility, and can under mild conditions, carry out polyelectrolyte complex reaction formation microcapsule, are widely used.Publication number is that CN101348781A patent discloses a kind of microcapsule that are embedded with immobilized dominant bacteria, and is applied to the processing to wood pulp papermaking waste water, obtains good effect.My unit publication number is that CN201250141Y patent discloses a kind of inner loop fluidized bed reactor reactor that uses microcapsule carrier, makes microcapsule carrier be applied to biological fluidized bed and carries out wastewater treatment and become possibility.But existing entrapping method technology is poor because of its mass-transfer performance, and stability is high and affect the application of microcapsule carrier.
Three, summary of the invention
For overcoming the deficiencies in the prior art, the invention discloses a kind of preparation method of the embedding dominant degradation bacteria bio-microcapsule that is applied to biological fluidized bed.
The object of the invention is to: provide technique method simple, with low cost, prepare good biocompatibility, mass-transfer performance is good, physical strength is high, the bio-microcapsule of the liquid core embedding dominant degradation bacteria of good stability.
The novel biology microcapsule preparation method of above-mentioned biological fluidized bed of the present invention, comprises the following steps:
(1) preparation of microorganism mixed solution: add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A in sodium alginate soln, wherein sodium alginate massfraction is 2.0%, tween 80 concentration is 0.5%～1.0%, Powdered Activated Carbon 0.75% (W/V); In solution A, adding 10%～20% (V/V) dominant degradation bacteria bacterium liquid mixes and obtains solution B;
(2) solution B is added drop-wise to injection system by peristaltic pump in 4% calcium chloride solution and reacts 15min～30min, make calcium alginate plastic beads;
(3) calcium alginate plastic beads that makes is filtered, and by sterile water wash; Then be placed in pH6.0,1.8% chitosan solution reacts 10min～20min and makes alginate calcium/chitosan microcapsules;
(4) alginate calcium making/chitosan microcapsules is filtered to the rear sterile water wash of using; Then the sodium citrate solution that is placed in 55mmol/L liquefies, and to make kernel be liquid microcapsule to 5min-12min;
(5) microcapsule that make are filtered to the rear sterile water wash of using, be then placed in sodium alginate soln overlay film 10min～20min again of 0.15%, make bio-microcapsule;
(6) microcapsule filtered and clean with pure water, then microcapsule being placed in to calcium ion solubility and being 0.05～0.1% physiological saline storage solution and preserve.
Compared with prior art, the invention has the advantages that in efficient degrading bacteria embedding and microcapsule, thereby effectively improved the treatment effect of efficient degrading bacteria to waste water, reduced the loss of microorganism; Secondly, microcapsule of the present invention have advantages of traditional sodium alginate micro gel capsule: good biocompatibility, and cheap and easy to get, on molecular chain, there is a large amount of carboxyls, can under mild conditions, form polyelectrolyte film by positive and negative electric charge inspiration and many range of polycationic substances; Again, the present invention has added appropriate Powdered Activated Carbon in capsule material, it has very large specific surface area and pore volume, utilize the adsorption of Powdered Activated Carbon, can make more micromolecular matrix degradation and dissolved oxygen be adsorbed on the one hand enters microcapsule and is concentrated in activated carbon surface and surrounding, for the Metabolic activity of microorganism is built good microenvironment, accelerate organic degradation process, also help on the other hand the mass-transfer performance that improves microcapsule of the present invention, reach better degradation effect.The maximum molecular weight cut-off of the made microcapsule of the present invention is about PEG4000, the small-molecule substance that relative molecular mass is less than to PEG1500 has good mass-transfer performance, microcapsule are stable under acid and most of divalent cation condition in wastewater treatment process, and monovalent cation and most of negatively charged ion are unfavorable for the stable of microcapsule.。Finally, the diameter of microcapsule is 3.0～4.0mm, and thickness is 10～20 μ m, and density is 1.05～1.08g/ml, can greatly reduce the energy consumption of biological fluidized bed and solve the blockage problem of fluidized-bed.
The preparation technology of microcapsule of the present invention is simple, and cost is low; In the time being applied to biological fluidized bed processing organic wastewater with difficult degradation thereby, its operation is also very convenient, directly the microcapsule of embedding dominant bacteria is added in biological fluidized bed and just can be moved, do not need significant investment, treatment effect is good, and kinetic equation loss is low, can meet environmental requirement.Microcapsule of the present invention can reuse in fluidized-bed, also can reclaim wherein dominant bacteria with simple method liquefaction, can not produce bad air and mud in technological process, are conducive to the protection of ecotope.
Four, embodiment
Embodiment 1:
A kind of novel biology microcapsule carrier of biological fluidized bed, it is characterized in that described microcapsule are made up of kernel and adventitia, its kernel is aqueous sodium alginate gel, in described sodium alginate gel, include Powdered Activated Carbon and immobilized dominant bacteria, kernel is coated with chitosan, chitosan is coated with sodium alginate film, and the embedding bacterium in inner nuclear material is orthodichlorobenzene efficient degrading bacteria.
The microcapsule carrier of the present embodiment is to prepare with step by the following method:
(1) preparation of microorganism mixed solution: add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A in sodium alginate soln, wherein sodium alginate massfraction is 2.0%, and tween 80 concentration is 0.5%, Powdered Activated Carbon 0.75%; In A, in solution A, adding 10%～20% (V/V) orthodichlorobenzene efficient degrading bacteria bacterium liquid mixes and obtains solution B;
(2) solution B is added drop-wise to injection system by peristaltic pump in 4% calcium chloride solution and reacts 20min, make sodium alginate microcapsule;
(3) sodium alginate microcapsule that makes is filtered, and by sterile water wash; Then be placed in 1.8% chitosan solution (pH value is 6.0) reaction 10min, make alginate calcium/chitosan microcapsules;
(4) alginate calcium making/chitosan microcapsules is filtered to the rear sterile water wash of using; Then the sodium citrate solution that is placed in 55mmol/L liquefies, and to make kernel be liquid micro-capsule to 8min;
(5) micro-capsule making is filtered to the rear sterile water wash of using, be then placed in 0.15% sodium alginate soln overlay film 10min again, make bio-microcapsule;
(6) microcapsule filtered and clean with pure water, then microcapsule being placed in to calcium ion solubility and being 0.1% physiological saline storage solution and preserve.
The above-mentioned orthodichlorobenzene efficient degrading bacteria bio-microcapsule input biological fluidized bed that is embedded with making is processed orthodichlorobenzene waste water, in biological fluidized bed, microcapsule carrier adds 15%, operational conditions: pH value is 7.0, aeration rate 120L/h, hydraulic detention time 2d.Get water sample one time every 4h, by gas chromatography determination orthodichlorobenzene concentration, to judge treatment effect, analytical data is as shown in table 1:
Orthodichlorobenzene Inlet and outlet water concentration monitor value in table 1: embodiment 1
Embodiment 2:
A kind of novel biology microcapsule carrier of biological fluidized bed, it is characterized in that described microcapsule are made up of kernel and adventitia, its kernel is aqueous sodium alginate gel, in described sodium alginate gel, include Powdered Activated Carbon and immobilized dominant bacteria, kernel is coated with chitosan, chitosan is coated with sodium alginate film, and the embedding bacterium in inner nuclear material is para-chlorophenol efficient degrading bacteria.
The microcapsule carrier of the present embodiment is to prepare with step by the following method:
(1) preparation of microorganism mixed solution: add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A in sodium alginate soln, wherein sodium alginate massfraction is 2.0%, and tween 80 concentration is 0.5%, Powdered Activated Carbon 0.75%; In solution A, adding 10%～20% (V/V) para-chlorophenol efficient degrading bacteria wet thallus mixes and obtains solution B;
(2) solution B is added drop-wise to injection system by peristaltic pump in 4% calcium chloride solution and reacts 20min, make sodium alginate microcapsule;
(3) sodium alginate microcapsule that makes is filtered, and by sterile water wash; Then be placed in 1.8% chitosan solution (pH value is 6.0) reaction 10min, make alginate calcium/chitosan microcapsules;
(4) alginate calcium making/chitosan microcapsules is filtered to the rear sterile water wash of using; Then the sodium citrate solution that is placed in 55mmol/L liquefies, and to make kernel be liquid micro-capsule to 8min;
(5) micro-capsule making is filtered to the rear sterile water wash of using, be then placed in 0.15% sodium alginate soln overlay film 10min again, make bio-microcapsule;
(6) microcapsule filtered and clean with pure water, then microcapsule being placed in to calcium ion solubility and being 0.1% physiological saline storage solution and preserve.
The above-mentioned para-chlorophenol efficient degrading bacteria bio-microcapsule carrier input biological fluidized bed that is embedded with making is processed para-chlorophenol waste water, in biological fluidized bed, microcapsule carrier adds 15%, operational conditions: pH value is 7.0, aeration rate 120L/h, hydraulic detention time 2d.Get water sample one time every 4h, by gas chromatography determination para-chlorophenol concentration, to judge treatment effect, analytical data is as shown in table 2:
Para-chlorophenol Inlet and outlet water concentration monitor value in table 2: embodiment 1
Claims (3)
1. one kind is applied to the preparation method of the novel biology microcapsule of biological fluidized bed, it is characterized in that described microcapsule are made up of kernel and adventitia, its kernel is aqueous sodium alginate gel, in described sodium alginate gel, include Powdered Activated Carbon and immobilized dominant degradation bacteria, kernel is coated with chitosan, and chitosan is coated with sodium alginate adventitia;
Wherein, described novel biology microcapsule preparation method, comprises the following steps:
(1) preparation of microorganism mixed solution: add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A in sodium alginate soln, wherein sodium alginate massfraction is 2.0%, and tween 80 concentration is 0.5%, Powdered Activated Carbon 0.75%(W/V); In solution A, adding 10%～20%(V/V) dominant degradation bacteria bacterium liquid mixes and obtains solution B, and wherein said dominant degradation bacteria is orthodichlorobenzene efficient degrading bacteria;
(2) solution B is added drop-wise to injection system by peristaltic pump in 4% calcium chloride solution and reacts 20min, make calcium alginate plastic beads;
(3) calcium alginate plastic beads that makes is filtered, and by sterile water wash; Then be placed in pH6.0,1.8% chitosan solution reacts 10min and makes alginate calcium/chitosan microcapsules;
(4) alginate calcium making/chitosan microcapsules is filtered to the rear sterile water wash of using; Then the sodium citrate solution that is placed in 55mmoI/L liquefies, and to make kernel be liquid microcapsule to 8min;
(5) microcapsule that make are filtered to the rear sterile water wash of using, be then placed in 0.15% sodium alginate soln overlay film 10min again, make bio-microcapsule;
(6) microcapsule filtered and clean with pure water, then microcapsule being placed in to calcium ion solubility and being 0.1% physiological saline storage solution and preserve.
2. the preparation method of the novel biology microcapsule that is applied to biological fluidized bed as claimed in claim 1, the microcapsule diameter that it is characterized in that preparation is 3.0～4.0mm, thickness is 10～20 μ m, and density is 1.05～1.08g/ml, and maximum machine intensity can reach 730.31g/cm
2, maximum molecular weight cut-off is PEG4000, the small-molecule substance that relative molecular weight is less than PEG1500 can freely pass through cyst membrane.
3. the application of the bio-microcapsule that prepared by the preparation method of an employing novel biology microcapsule that is applied to biological fluidized bed as claimed in claim 1 on various organic wastewater with difficult degradation thereby are processed, it is characterized in that microcapsule are stable under acid and most of divalent cation condition, and monovalent cation and most of negatively charged ion are unfavorable for the stable of microcapsule, biological fluidized bed aerating wastewater amount 80～140L/h, hydraulic detention time 2～3d.
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| CN201110183304.2A CN102351320B (en) | 2011-07-01 | 2011-07-01 | Method for preparing novel biological microcapsule for biological fluidized bed |
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| CN201110183304.2A CN102351320B (en) | 2011-07-01 | 2011-07-01 | Method for preparing novel biological microcapsule for biological fluidized bed |
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| CN102921359B (en) * | 2012-11-08 | 2014-06-11 | 山东轻工业学院 | Preparation method of biological capsule for sewage treatment |
| CN103275966B (en) * | 2013-06-04 | 2014-10-22 | 国家粮食局科学研究院 | Method for preparing coating microcapsule before microbial fermentation by utilizing common fermentation tank |
| CN104030457B (en) * | 2014-05-21 | 2016-02-03 | 东莞市华中生物科技有限公司 | The purifying eutrophic water method of using microbe filler and fluidized-bed |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616656A (en) * | 2003-11-10 | 2005-05-18 | 中国科学院大连化学物理研究所 | Method for preparing fixed yeast cell micro capsule |
| JP4048995B2 (en) * | 2002-05-23 | 2008-02-20 | トヨタ自動車株式会社 | Motor drive device, motor drive device control method, and computer-readable recording medium storing a program for causing a computer to execute control of the motor drive device |
| CN101134955A (en) * | 2007-08-02 | 2008-03-05 | 中国石油化工股份有限公司 | Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same |
| CN101348781A (en) * | 2008-09-18 | 2009-01-21 | 中南林业科技大学 | Micro-capsule embedded with immobilized dominant bacteria, preparation and use thereof |
| CN101402489A (en) * | 2008-11-14 | 2009-04-08 | 盐城工学院 | Process for treating chemical industry tail water with immobilized microorganism-artificial wet land |
| CN101875928A (en) * | 2009-04-29 | 2010-11-03 | 中国环境科学研究院 | Embedding immobilization method for microbial preparation |
| CN102051353A (en) * | 2009-11-04 | 2011-05-11 | 中国科学院大连化学物理研究所 | Spongiform stents-encapsulated microcapsules, preparation and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0363981A (en) * | 1989-07-31 | 1991-03-19 | Canon Inc | Information recording and reproducing device |
| JPH048995A (en) * | 1990-04-24 | 1992-01-13 | Fujikura Rubber Ltd | Fluid cutoff device |
| JPH0448995A (en) * | 1990-06-15 | 1992-02-18 | Tosoh Corp | Water treatment |
-
2011
- 2011-07-01 CN CN201110183304.2A patent/CN102351320B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4048995B2 (en) * | 2002-05-23 | 2008-02-20 | トヨタ自動車株式会社 | Motor drive device, motor drive device control method, and computer-readable recording medium storing a program for causing a computer to execute control of the motor drive device |
| CN1616656A (en) * | 2003-11-10 | 2005-05-18 | 中国科学院大连化学物理研究所 | Method for preparing fixed yeast cell micro capsule |
| CN101134955A (en) * | 2007-08-02 | 2008-03-05 | 中国石油化工股份有限公司 | Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same |
| CN101348781A (en) * | 2008-09-18 | 2009-01-21 | 中南林业科技大学 | Micro-capsule embedded with immobilized dominant bacteria, preparation and use thereof |
| CN101402489A (en) * | 2008-11-14 | 2009-04-08 | 盐城工学院 | Process for treating chemical industry tail water with immobilized microorganism-artificial wet land |
| CN101875928A (en) * | 2009-04-29 | 2010-11-03 | 中国环境科学研究院 | Embedding immobilization method for microbial preparation |
| CN102051353A (en) * | 2009-11-04 | 2011-05-11 | 中国科学院大连化学物理研究所 | Spongiform stents-encapsulated microcapsules, preparation and application thereof |
Non-Patent Citations (6)
| Title |
|---|
| 响应面法优化海藻酸钠-壳聚糖-粉末活性炭生物微胶囊制备工艺;杨唐仪，等;《化学通报》;20110318;第74卷(第3期);252-258 * |
| 李朝霞.海藻酸钠-壳聚糖-粉末活性炭生物微胶囊的传递性能研究.<<化学通报>>.2007,228-232. |
| 杨唐仪，等.响应面法优化海藻酸钠-壳聚糖-粉末活性炭生物微胶囊制备工艺.《化学通报》.2011,第74卷(第3期),252-258. |
| 气升式内循环生物微胶囊流化床的结构设计及对氯苯酚废水处理;许琦,等;<<盐城工学院学报(自然科学版) >>;20090331;第22卷(第1期);6-11 * |
| 海藻酸钠-壳聚糖-粉末活性炭生物微胶囊的传递性能研究;李朝霞;<<化学通报>>;20070331;228-232 * |
| 许琦,等.气升式内循环生物微胶囊流化床的结构设计及对氯苯酚废水处理.<<盐城工学院学报(自然科学版) >>.2009,第22卷(第1期),6-11. |
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