CN102342496A - Preparation method of cordyceps militaris natto product - Google Patents
Preparation method of cordyceps militaris natto product Download PDFInfo
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- CN102342496A CN102342496A CN2010102403155A CN201010240315A CN102342496A CN 102342496 A CN102342496 A CN 102342496A CN 2010102403155 A CN2010102403155 A CN 2010102403155A CN 201010240315 A CN201010240315 A CN 201010240315A CN 102342496 A CN102342496 A CN 102342496A
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- natto
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- cordyceps militaris
- caterpillar fungus
- chinese caterpillar
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Abstract
The invention discloses a preparation method of a cordyceps militaris natto product, belonging to the field of microorganism, comprising the following steps: crushing first-class soybean, wetting the crushed first-class soybean with water, and autoclaving; cooling the material, inoculating Bacillus natto liquid spawn, uniformly mixing, then keeping warm and carrying out fermentation, discharging, and drying; and crushing dried natto fermented material, then adding cordyceps militaris fruiting body powder proportionally, and uniformly mixing to obtain the cordyceps militaris natto product. Compared with pure natto powder, the prepared cordyceps militaris natto product has more stable activity of nattokinase, less peculiar smell, and better mouthfeel, and has high nutritional value and health care function.
Description
Technical field
The present invention relates to a kind of preparation method of northern Chinese caterpillar fungus natto product, belong to microorganism field.
Background technology
North Chinese caterpillar fungus and Cordyceps sinensis be Cordyceps belong to the xenogenesis fungi together, both chemical compositions, nutritional labeling and medicinal function are all closely similar.The main medicinal ingredient cordycepin equal size of north Chinese caterpillar fungus is far above wild cordyceps, and is easier to artificial cultivation than Cordyceps sinensis, therefore is chosen as the best substitute of Cordyceps sinensis and carries out a large amount of artificial cultures.Tame northern Chinese caterpillar fungus is that Cordyceps Militaris is grown in the fungus sporophore of growing on the dead organic matter and forming, and normally is primary raw material with the rice, processes through technologies such as profit water, sterilization, inoculation, cultivation, drying and pulverizing.Since the nineties in 20th century; Medicineization, pharmacology, toxicity and toxicity etc. to as the northern Chinese caterpillar fungus of medicine and health food have been carried out a large amount of research and safety evaluation; Proving that tame northern Chinese caterpillar fungus has no side effect, is safe and reliable as medicine and food use to health.
Natto is that outward appearance has thin tunica albuginea Bacillus natto and do not lose the fermentation article of soybean particle shape, and is direct-edible, but also freeze-drying, oven dry back eat, also can extract effective active matter respectively and process capsule and tablet.Natto mainly is that raw material is made with the soybean.The protein of soybean has indissolubility, and after making natto, the part that becomes is solvable and produce free amino acid, and non-existent various ferment can help stomach to digest and assimilate because Bacillus natto and related bacterium produce in the raw material.It is reported that Bacillus natto has the sterilizing effect to Escherichia coli.Often eat natto and all can play prevention and alleviation and therapeutic action cancer, diabetes, hypertension, artery sclerosis, obesity etc.
The medicated food formula that Cordyceps militaris fruiting body and food and Chinese herbal medicine compatibility form in state-owned hundreds of more than, but do not appear in the newspapers as yet with the production technology of natto compatibility.
Summary of the invention
The objective of the invention is to remedy the deficiency of above-mentioned prior art, a kind of preparation method of northern Chinese caterpillar fungus natto product is provided.
Realize that the technical scheme that the object of the invention adopted is:
A kind of preparation method of northern Chinese caterpillar fungus natto product may further comprise the steps:
(1) first-class soybean is pulverized after, be 1: 0.8～1 ratio profit water in the mass ratio of first-class soybean and water, under 0.10MPa～0.14MPa pressure, sterilize then;
(2) material after will sterilizing is cooled to 40～70 ℃, and the inoculated bacteria cell number is 1.0 * 10
7～6.0 * 10
7Mix behind the bafillus natto liquid spawn of individual/ml, the bafillus natto liquid spawn quality of inoculation is 4～25% of a first-class soybean quality, and at 37～40 ℃ of heat-preservation fermentations, discharging then is dried to water content and is no more than 10%;
(3) dried natto fermentate is crushed to 60～80 orders; Obtain natto powder, add 60～80 purpose Cordyceps militaris fruiting body powder then, the addition of Cordyceps militaris fruiting body powder be Cordyceps militaris fruiting body powder and natto powder gross mass 8～10%; Mix, promptly obtain northern Chinese caterpillar fungus natto product.
Be that first-class soy meal is broken to 5～8 orders in the step (1).
Sterilization time is 45～60 minutes described in the step (1).
The preparation method of bafillus natto liquid spawn is described in the step (2): the bacteria culture media after will dissolving pack into test tube, seal; After under 0.10MPa～0.14MPa, sterilizing; Put into the inclined-plane; The inoculation bafillus natto is purebred, under 32～40 ℃ of constant temperatures, cultivates then and covers with the inclined-plane until bacterial classification; Fluid nutrient medium is sterilized under 0.10MPa～0.14MPa pressure, inoculate slant strains after being cooled to 35～40 ℃, cultivate down at 37～40 ℃ then, reach 1.0 * 10 until the bacterial cell number
7～6.0 * 10
7Stop behind individual/ml cultivating; The pH value of said fluid nutrient medium is 7.0～7.2, and its raw material is peptone, beef extract, NaCl and water, peptone: beef extract: NaCl: the amount ratio of water is 2～5g: 1～3g: 1～5g: 1000ml.
The time of heat-preservation fermentation described in the step (2) is 24～36 hours.
After the discharging, be dried to water content at 40～60 ℃ and be no more than 10% in the step (2).
Compared with prior art, the present invention has the following advantages:
(1) experiment is found; Under identical preservation condition; Compare with simple natto powder; The natto kinase activity loss of northern Chinese caterpillar fungus natto product of the present invention obviously reduces; Make the shelf-life longer; Product quality is more stable, and some compositions that this result shows in the Cordyceps militaris fruiting body and contained have protective effect to natto kinase activity.In addition, contained superoxide dismutase (SOD) etc. has non-oxidizability in the Cordyceps militaris fruiting body, can reduce the oxidation of bafillus natto thalline, thereby the survival of this thalline is played a protective role.The bacterial cell number (1.0 * 10 of bafillus natto liquid spawn
7～6.0 * 10
7Individual/ml) inoculum concentration with this liquid spawn helps the breeding of bafillus natto in the sweat and the raising of natto kinase activity.
(2) adopt the northern Chinese caterpillar fungus natto product of the inventive method preparation to compare with the natto powder that does not add Cordyceps militaris fruiting body, its peculiar smell is little, and mouthfeel is better.
(3) Cordyceps militaris fruiting body have tangible calmness, antifatigue, antitumor, suppress cancer cell and impel effects such as 3male hormone secretion; But therefore enhancing body immunologic function etc. adopt the northern Chinese caterpillar fungus natto product of the inventive method preparation more comprehensive to the health care of human body than simple natto powder.
The specific embodiment
The present invention is further illustrated through specific embodiment below, but protection content of the present invention is not limited to following examples.
Embodiment 1:
(1) with peptone 5g, beef extract 3g, NaCl 5g, agar 15g and water 1000ml mixing and dissolve, use sodium bicarbonate solution adjust pH to 7.0 again, obtain bacteria culture media.Bacteria culture media after will the dissolving 18mm * 180mm test tube of packing into, each test tube 7ml, tampon behind the sterilization 30min, is put into the inclined-plane under 0.14MPa beyond the Great Wall.In superclean bench the inoculation bafillus natto purebred after, be cultured under 37 ℃ of constant temperatures cover with the inclined-plane till, obtain slant strains.
(2) with peptone 5g, beef extract 3g, NaCl 5g and water 1000ml mixing, use sodium bicarbonate solution adjust pH to 7.0 again, obtain fluid nutrient medium.With 500ml glass bottle fluid nutrient medium 100ml, tampon seals, and sterilization 30min under 0.14MPa is cooled to 35 ℃ of inoculation slant strains, every 500ml fluid nutrient medium inoculation 0.3cm
2, adopting reciprocal bottle swingging machine then, bottle speed of shaking is 140 times/min, under 37 ℃, shakes bottle and cultivates, the bacterial cell number is 5.83 * 10 until adopting the viable count method to make wherein
7Stop behind individual/ml cultivating, obtain the bafillus natto liquid spawn, be in store for.
(3) select first-class soybean for use, be crushed to behind 5～8 orders mass ratio in first-class soybean and water and be 1: 0.8 ratio profit water, then at sterilization 45min (under 0.10MPa～0.14MPa pressure, sterilize, temperature can reach more than 120 ℃ usually) 0.14MPa pressure under.
(4) material after will sterilizing is cooled to 40 ℃; Mix after inoculating above-mentioned bafillus natto liquid spawn, the bafillus natto liquid spawn quality of inoculation was 20% of a first-class soybean quality, 37 ℃ of heat-preservation fermentations 24 hours; Discharging then, being dried to water content at 40 ℃ is 9.5%.
(5) dried natto fermentate is crushed to 60 orders; Obtain natto powder, add 60 purpose Cordyceps militaris fruiting body powder then, the addition of Cordyceps militaris fruiting body powder is 10% of Cordyceps militaris fruiting body powder and a natto powder gross mass; Mix, promptly obtain northern Chinese caterpillar fungus natto product.
Through measuring; The natto kinase activity of this north Chinese caterpillar fungus natto product is 2450FU/g; The natto kinase activity that adds the preceding gained natto powder of Cordyceps militaris fruiting body powder is 2722FU/g; In 37 ℃ of insulating boxs, preserve after 90 days, the natto kinase activity of this north Chinese caterpillar fungus natto product is 2380FU/g, than decline 70FU/g before preserving; Descended 2.857%; And natto powder natto kinase activity decline 338FU/g under similarity condition has descended 12.417%, and fall is much larger than the former.
Please hear and above-mentioned northern Chinese caterpillar fungus natto product of everyone oral 2g and natto powder with nose at 25 years old～47 years old healthy male at 30 ages, the northern Chinese caterpillar fungus natto product peculiar smell of 30 bit test persons, 100% reflection is little as a result, and mouthfeel is better.
Gained north Chinese caterpillar fungus natto product is preserved under shady and cool, drying, ventilation condition through quality inspection, packing, warehouse-in.
Embodiment 2:
(1) with peptone 5g, beef extract 3g, NaCl 5g, agar 15g and water 1000ml mixing and dissolve, use sodium bicarbonate solution adjust pH to 7.0 again, obtain bacteria culture media.Bacteria culture media after will the dissolving 18mm * 180mm test tube of packing into, each test tube 7ml, tampon behind the sterilization 40min, is put into the inclined-plane under 0.12MPa beyond the Great Wall.In superclean bench the inoculation bafillus natto purebred after, be cultured under 37 ℃ of constant temperatures cover with the inclined-plane till, obtain slant strains.
(2) with peptone 5g, beef extract 3g, NaCl 5g and water 1000ml mixing, use sodium bicarbonate solution adjust pH to 7.0 again, obtain fluid nutrient medium.With 500ml glass bottle fluid nutrient medium 100ml, tampon seals, and sterilization 40min under 0.12MPa is cooled to 37 ℃ of inoculation slant strains, every 500ml fluid nutrient medium inoculation 0.2cm
2, adopting reciprocal bottle swingging machine then, bottle speed of shaking is 120 times/min, under 40 ℃, shakes bottle and cultivates, the bacterial cell number reaches 1.65 * 10 until adopting the viable count method to make wherein
7Stop behind individual/ml cultivating, obtain the bafillus natto liquid spawn, be in store for.
(3) select first-class soybean for use, be crushed to behind 5～8 orders mass ratio in first-class soybean and water and be 1: 1 ratio profit water, 60min then sterilizes under 0.1MPa pressure.
(4) material after will sterilizing is cooled to 50 ℃; Mix after inoculating above-mentioned natto gemma bar liquid spawn, the natto gemma bar liquid spawn quality of inoculation was 10% of a first-class soybean quality, 40 ℃ of heat-preservation fermentations 24 hours; Discharging then, being dried to water content at 50 ℃ is 10%.
(5) dried natto fermentate is crushed to 70 orders; Obtain natto powder, add 70 purpose Cordyceps militaris fruiting body powder then, the addition of Cordyceps militaris fruiting body powder be Cordyceps militaris fruiting body powder and natto powder gross mass 9%; Mix, promptly obtain northern Chinese caterpillar fungus natto product.
The natto kinase activity of present embodiment gained north Chinese caterpillar fungus natto product is 2698FU/ml.The natto powder that this north Chinese caterpillar fungus natto product and present embodiment is not added Cordyceps militaris fruiting body places insulating box to preserve, and equal conditions finds that down the former is littler than the latter's natto kinase activity loss.
By the method identical with embodiment 1, experimenter's 100% reflection is should north Chinese caterpillar fungus natto product peculiar smell little, and mouthfeel is better.
Gained north Chinese caterpillar fungus natto product is preserved under shady and cool, drying, ventilation condition through quality inspection, packing, warehouse-in.
Embodiment 3:
(1) with peptone 5g, beef extract 3g, NaCl 5g, agar 15g and water 1000ml mixing and dissolve, use sodium bicarbonate solution adjust pH to 7.2 again, obtain bacteria culture media.Bacteria culture media after will the dissolving 15mm * 150mm test tube of packing into, each test tube 4ml, tampon behind the sterilization 50min, is put into the inclined-plane under 0.1MPa beyond the Great Wall.In superclean bench the inoculation bafillus natto purebred after, be cultured under 36 ℃ of constant temperatures cover with the inclined-plane till, obtain slant strains.
(2) with peptone 2g, beef extract 3g, NaCl 4g and water 1000ml mixing, use sodium bicarbonate solution adjust pH to 7.2 again, obtain fluid nutrient medium.With 500ml glass bottle fluid nutrient medium 100ml, tampon seals, and sterilization 40min under 0.14MPa is cooled to 35 ℃ of inoculation slant strains, every 500ml fluid nutrient medium inoculation 0.4cm
2, adopting reciprocal bottle swingging machine then, bottle speed of shaking is 160 times/min, under 37 ℃, shakes bottle and cultivates, the bacterial cell number reaches 3.40 * 10 until adopting the viable count method to make wherein
7Stop behind individual/ml cultivating, obtain the bafillus natto liquid spawn, be in store for.
(3) select first-class soybean for use, be crushed to behind 5～8 orders mass ratio in first-class soybean and water and be 1: 0.9 ratio profit water, 50min then sterilizes under 0.12MPa pressure.
(4) material after will sterilizing is cooled to 40 ℃; Mix after inoculating above-mentioned natto gemma bar liquid spawn, the quality of inoculation natto gemma bar liquid spawn was 25% of a first-class soybean quality, 37 ℃ of heat-preservation fermentations 36 hours; Discharging then, being dried to water content at 55 ℃ is 8.0%.
(5) dried natto fermentate is crushed to 80 orders; Obtain natto powder, add 80 purpose Cordyceps militaris fruiting body powder then, the addition of Cordyceps militaris fruiting body powder be Cordyceps militaris fruiting body powder and natto powder gross mass 8%; Mix, promptly obtain northern Chinese caterpillar fungus natto product.
The natto kinase activity of present embodiment gained north Chinese caterpillar fungus natto product is 2780FU/g.The natto powder that this north Chinese caterpillar fungus natto product and present embodiment is not added Cordyceps militaris fruiting body places insulating box to preserve, and equal conditions finds that down the former is littler than the latter's natto kinase activity loss.
By the method identical with embodiment 1, experimenter's 100% reflection is should north Chinese caterpillar fungus natto product peculiar smell little, and mouthfeel is better.
Gained north Chinese caterpillar fungus natto product is preserved under shady and cool, drying, ventilation condition through quality inspection, packing, warehouse-in.
Claims (6)
1. the preparation method of a northern Chinese caterpillar fungus natto product is characterized in that may further comprise the steps:
(1) first-class soybean is pulverized after, be 1: 0.8～1 ratio profit water in the mass ratio of first-class soybean and water, under 0.10MPa～0.14MPa pressure, sterilize then;
(2) material after will sterilizing is cooled to 40～70 ℃, and the inoculated bacteria cell number is 1.0 * 10
7～6.0 * 10
7Mix behind the bafillus natto liquid spawn of individual/ml, the bafillus natto liquid spawn quality of inoculation is 4～25% of a first-class soybean quality, and at 37～40 ℃ of heat-preservation fermentations, discharging then is dried to water content and is no more than 10%;
(3) dried natto fermentate is crushed to 60～80 orders; Obtain natto powder, add 60～80 purpose Cordyceps militaris fruiting body powder then, the addition of Cordyceps militaris fruiting body powder be Cordyceps militaris fruiting body powder and natto powder gross mass 8～10%; Mix, promptly obtain northern Chinese caterpillar fungus natto product.
2. the preparation method of northern Chinese caterpillar fungus natto product according to claim 1 is characterized in that: be that first-class soy meal is broken to 5～8 orders in the step (1).
3. the preparation method of northern Chinese caterpillar fungus natto product according to claim 1 is characterized in that: sterilization time is 45～60 minutes described in the step (1).
4. the preparation method of northern Chinese caterpillar fungus natto product according to claim 1; The preparation method who it is characterized in that bafillus natto liquid spawn described in the step (2) is: the bacteria culture media after will dissolving pack into test tube, seal; After under 0.10MPa～0.14MPa, sterilizing; Put into the inclined-plane; The inoculation bafillus natto is purebred, under 32～40 ℃ of constant temperatures, cultivates then and covers with the inclined-plane until bacterial classification; Fluid nutrient medium is sterilized under 0.10MPa～0.14MPa pressure, inoculate slant strains after being cooled to 35～40 ℃, cultivate down at 37～40 ℃ then, reach 1.0 * 10 until the bacterial cell number
7～6.0 * 10
7Stop behind individual/ml cultivating; The pH value of said fluid nutrient medium is 7.0～7.2, and its raw material is peptone, beef extract, NaCl and water, peptone: beef extract: NaCl: the amount ratio of water is 2～5g: 1～3g: 1～5g: 1000ml.
5. the preparation method of northern Chinese caterpillar fungus natto product according to claim 1 is characterized in that: the time of heat-preservation fermentation described in the step (2) is 24～36 hours.
6. the preparation method of northern Chinese caterpillar fungus natto product according to claim 1 is characterized in that: after the discharging, be dried to water content at 40～60 ℃ and be no more than 10% in the step (2).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106387676A (en) * | 2016-09-19 | 2017-02-15 | 江苏师范大学 | Preparation method of active natto powder |
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| CN101757189A (en) * | 2010-03-11 | 2010-06-30 | 黑龙江省中医研究院 | Calcium supplement preparation |
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2010
- 2010-07-27 CN CN2010102403155A patent/CN102342496B/en not_active Expired - Fee Related
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| CN1647689A (en) * | 2004-01-19 | 2005-08-03 | 张溪水 | Method for producing natto food |
| CN1714675A (en) * | 2005-07-26 | 2006-01-04 | 李英姬 | Natto powder and preparing method and health food containing such natto powder |
| CN1957971A (en) * | 2006-11-02 | 2007-05-09 | 李英姬 | Health products for decreasing blood fat |
| CN101002608A (en) * | 2007-01-10 | 2007-07-25 | 孙景文 | Food adjuvant made of natto product and preserved fruit, and processing method therefor |
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| CN106387676A (en) * | 2016-09-19 | 2017-02-15 | 江苏师范大学 | Preparation method of active natto powder |
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