CN1714675A - Natto powder and preparing method and health food containing such natto powder - Google Patents
Natto powder and preparing method and health food containing such natto powder Download PDFInfo
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- CN1714675A CN1714675A CNA200510017008XA CN200510017008A CN1714675A CN 1714675 A CN1714675 A CN 1714675A CN A200510017008X A CNA200510017008X A CN A200510017008XA CN 200510017008 A CN200510017008 A CN 200510017008A CN 1714675 A CN1714675 A CN 1714675A
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Abstract
The present invention discloses a kind of natto powder, and the natto powder is prepared through the following steps: soaking soybean in water for 10-24 hr; steaming at temperature over 100 deg.c for 20-60 min and cooling the cooked soybean to 20-40 deg.c; spraying natto bacteria liquid and fermentation at 35-45 deg.c and 50-90 RH% for 18-30 hr; cold storing the fermented natto soybean with rich transparent baves at 0-10 deg.c for over 24 hr; and freeze drying, crushing and 40-80 mesh sieving. The application of the natto powder is also disclosed, and the natto powder may be applied in serial health foods with the health functions of dissolving thrombus, balancing blood pressure, preventing cardiac and cerebral vascular diseases, etc.
Description
Technical field
The present invention relates to a kind of natto powder and preparation method thereof and use, specifically be meant a kind of natto powder and preparation method and the health food that contains this natto powder with it
Background technology
Natto is a kind of functional food with long history.Studies show that natto has on the basis of the original nutritional labeling of soybean, because the action protein matter of Bacillus natto is decomposed into each seed amino acid, the content of vitamins increases significantly, thereby is very beneficial for digesting and assimilating.As what the Main Ingredients and Appearance of natto received that people pay close attention to Nattokinase, farnoquinone, pyridinedicarboxylic acid etc. are arranged.Nattokinase has the thrombus effect of highly significant after deliberation, has simultaneously to purify the blood, and repairs the effect of blood vessel with high quality protein matter.So the prevention to diseases such as hypertension and encephalatrophy, senile dementia, heart disease, artery sclerosis has obvious effects.But, the deep-processed food that contains natto in the market but seldom, the health products that especially contain natto are rare especially.
Summary of the invention
The objective of the invention is for a kind of natto deep-processed food with health care is provided, specifically is a kind of natto powder and preparation method thereof and the health foodstuff series that contains this natto powder.
Its technology contents:
A kind of preparation method of natto powder, be made up of the following step: the soya bean of getting after selected was soaked in water 10-24 hour; After it is fully steeped out, more than 100 ℃ and comprise under 100 ℃ of temperature boiling 20-60 minute; When the ripe beans after the boiling are cooled to 20-40 ℃, evenly spray into Bacillus natto liquid, cultivated 18-30 hour at temperature 35-45 ℃, relative humidity 50-90% condition bottom fermentation; Place in the 0-10 ℃ of refrigerating chamber refrigeration more than 24 hours the natto that ferments, produces more transparent haircuts; Take out, carry out freeze drying, pulverize, cross the 40-80 mesh sieve, promptly.
Promptly obtain product-natto powder of the present invention according to above-mentioned preparation method.
Bacillus natto among the preparation method of the present invention is some kinds or the natto bud pole bacterium (Bacillus natto) of withered grass bud withered grass bud pole bacterium (BacillusSubtilis), and Bacillus natto can be bought on market.
Natto powder of the present invention is applied in the concrete scheme of health food:
Contain one of health food scheme of natto powder of the present invention: form by following each component by weight:
Natto powder 60-80%, flavone of hawthorn fruit 15-25%, garlic powder 3-15%, superfine silica gel powder 2-5%.
The preparation method of above-mentioned flavone of hawthorn fruit gets a certain amount of dry leaves of Hawthorn to add 95% alcohol reflux extraction 3 times, 2h/ time, merge ethanol extract, be recycled to nothing alcohol flavor and add 10 times of water gagings stirring refrigeration placement 24h, the aqueous solution is crossed macroporous resin column, make eluent with ethanol, merge ethanol eluate, recovered under reduced pressure promptly gets described flavone of hawthorn fruit to doing.
The preparation method of above-mentioned garlic powder gets garlic, clean, freeze drying, pulverize garlic powder.
The preparation method who contains one of the health food scheme of natto powder of the present invention after above-mentioned each component is mixed according to quantity, carries out filling with No. 0 capsule, promptly.
3-4 grain of usage and consumption, one day 2-3 time.
The health food of one of this kind scheme is to be used for artery sclerosis, thrombus, coronary heart disease crowd's bread and cheese.Hereinafter referred to as " bean-containing capsule ".
It is edible that bean-containing capsule is suitable for the elderly, natto powder is longer than thrombus in its prescription, purify the blood, repair blood vessel, hawthorne leaf P.E-flavone of hawthorn fruit then is better than reducing the lipid components in the blood, both complement each other, learn from other's strong points to offset one's weaknesses, and whole prescription has thrombolysis, balancing blood pressure, the effect of prevention cardiovascular and cerebrovascular disease.
Prove its above-mentioned effect below by pharmacological evaluation.
Appendix 1: bean-containing capsule thrombus test report experimental technique and result:
1: the natto extract pharmacodynamics test
The preparation of natto extract: it is an amount of to get natto powder, and water extracts, precipitation with alcohol, the centrifugal natto extract that gets.
One, natto extract is to the influence of rabbit thrombus in vivo dissolving
Get 30 of large ear rabbits, male and female half and half, body weight 1.8 ± 0.2kg is divided into five groups at random, 6 every group, separate by only carrying out arteria carotis communis before the administration, dissociate long proximal part of about 2cm and distal end are clamped with parteriole, allow blood pass through in blood vessel, 1.5 after hour, thrombus forms in the arteria carotis, irritates stomach natto extract 76mg/kg to rabbit, 38mg/kg and 19mg/kg, the positive control drug group is by duodenum injection streptokinase capsule liquid 380mg/kg, the blank group is irritated stomach isometric(al) physiological saline, put to death rabbit after 5 hours, cut carotid artery vascular, cut blood vessel open with little scissors and take out silk thread and thrombus, it is long to measure 1cm, claim its weight, then thrombus be put in 60 ℃ of oven dry in the baking oven, the weight in wet base of calculating the arteria carotis thrombus and dry weight and with the physiological saline comparison, inspection has there was no significant difference, the results are shown in Table 1.
Table 1 natto extract is to the influence of rabbit thrombus in vivo dissolving
Group drug dose number of animals wet weight of thrombus wet weight of thrombus
(mg/kg) (the only) (mg/1cm of X ± SD) (mg/1cm of X ± SD)
Physiological saline-6 10.0 ± 4.73 4.80 ± 1.89
Thrombolysis capsule 380 6 4.42 ± 2.27
*1.92 ± 0.86
*
Natto extract 76 6 3.33 ± 0.68
*1.50 ± 0.55
* *
Natto extract 38 6 5.17 ± 1.37
*2.50 ± 0.84
*
Annotate:
*Compare P＜0.05 with the physiological saline group
*Compare P＜0.01 with the physiological saline group
* *Compare P＜0.001 with the physiological saline group
Natto extract 196 7.00 ± 4.22 3.50 ± 2.53
Table 1 data show, after forming arteria carotis communis thrombus rabbit filling stomach natto extract, no matter are the weight in wet base or the dry weight of thrombus, and the result all demonstrates thrombus tangible thrombolytic effect, and significantly finds out the dose dependent relation.The positive drug thrombolysis capsule has tangible dissolution to thrombus in the blood vessel.
Two, natto extract is to the dissolution of dog arterial thrombus
Get 30 of hybrid dogs, the male and female dual-purpose, body weight 10.1 ± 1.6kg is divided into five groups at random, 6 every group.By only dog being anaesthetized (30mg/kg) with the yellow Jackets vena femoralis injection, back of the body position is fixed on the dog autopsy table, cut off groin down to femoral artery place hair, the femoral artery of dog is isolated in the operation of sterilization back, clamp with artery clamp at the femoral artery two ends, the long blood vessel of about 3cm is stayed in the centre, wear a rhizoid line at this intravascular sewing-needle, the about 1.5cm of line length is with the hemostasis of cotton balls compressing blood vessel, open artery clamp, allow blood freely pass through, owing in the blood vessel one section silk thread is arranged, blood flow is crossed and can be formed thrombus, after 2 hours, under X close to monitor, to the general amine of intravascular injection artery cover radiography liquid flooding shadow, determine that blood vessel does not have blood and passes through in the upper end of femoral artery, thrombus forms, takes the X-ray sheet under the X-ray machine.
The dog of physiological saline control group is irritated stomach isometric(al) physiological saline, positive drug control group is by duodenum injection thrombolysis capsule liquid 300mg/kg, administration group per os is respectively irritated stomach natto extract 50mg/kg, 25mg/kg, 12.5mg/kg, 5 hours, under X-ray, inject the general amine of general shadow once more, observing thrombus has and does not have dissolvedly, and whether blood passes through, and takes the X-ray sheet.
The result: 1,6 dog thrombus of natto extract high dose group (50mg/kg) are all dissolved falls, and radiography shows that blood passes through smoothly.
2, the thrombus of 6 dogs of dosage group (25mg/kg) is all dissolved in the natto extract, and injecting contrast shows that blood passes through smoothly.
3, have 4 routine blood to pass through smoothly in 6 dogs of natto extract low dose group (12.5mg/kg), have 1 routine radiography to show that blood does not pass through, 1 routine display part thrombus is dissolved, has blood to pass through.
4,6 dogs of thrombolysis capsule group (300mg/kg) wherein have 2 routine thrombus all dissolved, and contrast solution passes through smoothly, have 2 routine contrast solutions partly to pass through, and remaining 2 routine thrombus are not dissolved, and no contrast solution passes through.
Appendix 2: bean-containing capsule is regulated the blood fat test report
1 material and method
1.1 sample
Bean-containing capsule is provided by grass elixir industry Co., Ltd of Yanbian University, and being tried thing is capsule, every 0.45 gram, examination by sensory organs no abnormality seen.The human body RD is everyone gram every days 2.7.
1.2 TC, TG, HDL-C kit
Beijing Zhongsheng Biological Engineering High Technology Company produces.
1.3 animal used as test
50 of the male wistar rats that provides by institute of oncology, Heilongjiang Province Experimental Animal Center, body weight 180 ± 20 grams, lot number is 02004152.
1.4 dosage design
The human body recommended amounts of bean-containing capsule is everyone 2.7g every day, become body weight for humans in 60kg, then be equivalent to the 45mg/kg body weight, the dose,equivalent of rat is 10 times of human body recommended amounts, be 450mg/kg body weight (middle dosage), other establishes two dosage groups, 1350mg/kg body weight (high dose) and 225mg/kg body weight (low dosage), and each treated animal is all irritated the stomach volume by the 20ml/kg body weight and is irritated stomach.
1.5 high lipid food basal feed 93.8%, cholesterol 1%, lard 5%, cholate 0.2%.
1.6 experimental technique
With basal feed feed raise rat and observe 3 days after, get tail blood, enzymatic assays serum total cholesterol (TC), triglycerides (TG), HDL-C (HDL-C), according to the TC level animal is divided into 5 groups at random: 3 are subjected to examination group (225,450,1350mg/kg body weight), high lipid food control group, basal feed control group.Begin from formal experiment, respectively group is used high lipid food instead, is tried thing filling stomach and gives, and the single cage of animal is raised, and freely drinks water and writes down the weight of animals weekly, after the filling stomach gives 30 days, gets the every blood lipids index of tail hematometry.
1.7 experimental data is handled with the LKAT software analysis.
2 experimental results and analysis
2.1 bean-containing capsule is to the influence of rat body weight
Table 2 bean-containing capsule is to the influence of rat body weight
After testing before grouping dosage (mg/kg) number of animals (only) body weight (g) weightening finish (g) test
1350 10 193.0±20.7 231.0±22.5 38.0±19.2
450 10 206.0±14.8 250.0±28.3 36.1±14.6
225 10 202.0±13.4 239.0±21.1 37.1±10.9
High fat control group 10 198.0 ± 26.5 234.0 ± 19.9 43.8 ± 18.3
Basal feed control group 10 208.0 ± 18.6 231.0 ± 19.2 23.4 ± 19.5
By table 2 as seen, with 1350,450, the bean-containing capsule (prescription 1) of 225mg/kg.bw dosage irritates stomach and gave rat 30 days, each treated animal growth, movable normal, each dosage group and high fat animals of control group body weight and basal feed control group are relatively, the body weight of each dosage treated animal has growth trend with basal feed control group ratio, but does not have conspicuousness difference (P＞0.05).
2.2 bean-containing capsule is to the influence of Serum TC, TG, HDL-C
2.2.1 bean-containing capsule sees Table 3 to the influence of rat blood serum T-CHOL (TC)
Table 3 bean-containing capsule (prescription 1) is to the influence of rat blood serum T-CHOL (TC)
(mmol/L) experiment back (mmol/L) before group (mg/kg) experiment
1350 1.664±0.2559 1.866±0.3894
**
450 1.657±0.2612 1.889±0.5192
**
225 1.649±0.2435 2.073±0.2363
*
High fat control group 1.672 ± 0.2447 2.498 ± 0.2949
Basal feed control group 1.675 ± 0.2676 1.633 ± 0.3010
*
Annotate:
*Compared utmost point marked difference, P＜0.01 with high fat control group
*Compared marked difference, P＜0.05 with high fat control group
2.2.2 bean-containing capsule is to the influence of rat blood serum triglycerides (TG)
Bean-containing capsule sees Table 4 to the influence of rat blood serum triglycerides (TG).
Table 4 bean-containing capsule is to the influence of rat blood serum triglycerides (TG)
(mmol/L) experiment back (mmol/L) before group (mg/kg) experiment
1350 1.028±0.1789 1.321±0.2537
*
450 1.064±0.2006 1.488±0.3416
225 1.042±0.1648 1.507±0.1936
High fat control group 1.068 ± 0.2837 1.743 ± 0.4836
Basal feed control group 1.079 ± 0.2195 1.151 ± 0.4702
*
Annotate:
*Compared marked difference, P＜0.05 with high fat control group
2.2.3 bean-containing capsule is to the influence of rat blood serum HDL-C (HDL-C)
Table 5 bean-containing capsule is to the influence of rat blood serum HDL-C (HDL-C)
(mmol/L) experiment back (mmol/L) before group (mg/kg) experiment
1350 1.017±0.1881 1.216±0.0523
450 1.059±0.1535 1.205±0.0811
225 1.029±0.1673 1.263±0.0882
High fat control group 1.099 ± 0.1644 1.206 ± 0.587
Basal feed control group 1.063 ± 0.1286 1.219 ± 0.1142
By table 3,4,5 as seen, with 1350,450, the bean-containing capsule of 225mg/kg dosage irritates after stomach gives 30 days, the serum TC of basal feed control group is lower than high fat control group all the time with TG, and utmost point marked difference or marked difference are arranged, and illustrates that hyperlipidemia model is successful.The serum TC of high, middle dosage group and the apparent in view reduction of high fat control group (P＜0.01), low dose group and high fat control group ratio, the reduction amplitude has marked difference P＜0.05, the TG of high dose group serum and high fat control group ratio, obviously reduce P＜0.05, the HDL-C of each dosage group serum and high fat control group relatively do not have obvious rising.
2.3 bean-containing capsule is to the influence of rat fat level
Table 6 bean-containing capsule is to the influence of rat fat level
Dosage (mg/kg) TC (%) TG (%)
1350 24.4 24.2
450 24.3 14.6
225 17.0 13.5
Can see that from above result during to experiment end in the 30th day, the TC of experimental group, TG and the apparent in view reduction of high fat control group have marked difference or utmost point marked difference P＜0.05 or P＜0.01 respectively.Experiment shows that bean-containing capsule has the effect of tangible reduction cholesterolemia and triglycerides.
3 conclusions
This tests high fat control group all the time than serum TC, the TG height of basal feed control group, and the conspicuousness difference is arranged, and illustrates that hyperlipidemia model is successful, credible result.With 225,450, the bean-containing capsule of 1350mg/kg dosage irritates stomach and gave rat 30 days, the serum cholesterol of each dosage treated animal, the triglyceride levels of high dose group reduce than obvious with high fat control group, the high density lipoprotein level of serum of each dosage treated animal and high fat control group ratio all do not have obviously rising.The result shows that the laboratory sample bean-containing capsule has the adjusting blood fat.
Contain natto powder of the present invention the health food scheme two: form by following each component by weight:
Natto powder 70-80%, isoflavones 3-5%, Brazil mushroom crude polysaccharide 17-25%.Above-mentioned isoflavones can be bought by market; Brazil mushroom crude polysaccharide is that the Ji Songrong water is extracted three times, adds ethanol to 95% after concentrating and carries out alcohol precipitation, and pure hypostasis drying is Brazil mushroom crude polysaccharide.Two preparation method of health food scheme after according to quantity natto powder, isoflavones, Agaricus Blazei Murrill polysaccharide mixed, carries out filling with No. 0 capsule, promptly.
Two health food of this kind scheme is the health food that is used for women's climacteric syndrome and dysmenorrhoea.Contain isoflavones, Vitamin E etc. in the natto powder, these compositions are easier being absorbed by the body under the effect of Bacillus natto.Isoflavones is typical phytoestrogen, and isoflavones can replace estrogen to combine the performance estrogen-like action with ER, can disturb estrogen to combine with ER again, shows as the effect of antiestrogenic sample.Isoflavones shows that estrogen active or antiestrogenic activity depend primarily on the hormone metabolism state of study subject itself.To the horizontal person of high estrogen,, show the antiestrogenic activity as the animal and the young woman of young animal and estrogenization; To the estrogen level junior,, show estrogen active as brood, removal ovary animal, menopausal women.The estrogen-like action of isoflavones is withdrawn relevant disease such as blood fat rising, atherosclerotic and osteoporosis etc. to elderly woman is many with hormone, and certain prevention and therapeutic action are arranged.This product can be prevented the generation of women's reproduction product cancer that hormonal balance imbalance causes.Contain Agaricus Blazei Murrill polysaccharide among the Ji Songrong and have the generation that the enhancing immunization helps preventing and treating women's climacteric syndrome generation and genital organ tumour.
Two health food of this kind scheme is with 23 routine climacteric metancholia women's effect observation
Research object and condition: menopause symptom is arranged, healthy women 23 examples of no endocrine system disease.At the age 46.00 ± 2.5, the estrogens medicine was not used in all women's medications in preceding 3 months.
Administrated method: 4/time, 2 times/day, continuous oral 25-30 day is people course of treatment, totally 2 courses of treatment.
Observation item:
Kupperman improvement scoring method is adopted in the menopause symptom scoring, and the every month record in before the treatment and treatment back once.Blood FSH, each is measured once before and after the E2 treatment.The Ultrasonic Detection endometrium thickness, each is measured once before and after the treatment.Each is checked once before and after blood pressure, body weight, the breast treatment.Efficacy evaluation: kunpperman point system index: Tidal fever with perspiration 4 minutes, cacesthesia, insomnia, emotional each 2 minutes depressedly are suspicious of, dizzy, tired, osteoarthrosis pain, headache, palpitaition, the sense of skin ant 1 minute, sexual life difficulty, urinary infection each 2 minutes.Degree: asymptomatic symptom is 0 minute, and idol had symptom 1 minute, symptom was often arranged 2 minutes, and influence is lived and worked final score=index * degree 3 fens.
Efficacy evaluation: alleviate fully: overall score descends 〉=80%; Produce effects: overall score descends 〉=50%; Effectively: overall score descends 〉=20%; Invalid: overall score descends≤20%.
The result: show according to kupperman scoring analysis result: alleviation accounts for 61.74% fully, and produce effects 20 examples account for 29.41, and effective 4 examples account for 5.88%, and invalid 2 examples account for 2.94, and total effective rate is 97.06%.
Contain natto powder of the present invention the health food scheme three: form by following each component by weight: natto powder 60-85%, calcium lactate 15-40%.
Described calcium lactate can be bought by market.Natto powder and calcium lactate mixed powder are packaged into bag and get final product.
Owing in natto powder, add calcium lactate, calcium is formed with during the fermentation be beneficial to the compound calcium of gastrointestinal absorption.Therefore the scope of application of three of this kind scheme health food: the crowd that osteoporosis need be replenished the calcium.
Usage: a 1-2 bag, every bag of 5g, one day 2 times.
Contained farnoquinone in the natto, isoflavones and polyglutamic acid are for the absorption of the calcium of human body, γ-the hydroxylating of BMP suppresses osteoclast absorption again of calcium from bone and all have certain effect, and these effects all helps prevention and treatment osteoporosis.More farnoquinone is arranged in the natto, and every 50g in the commodity natto contains farnoquinone 870mg.Human oral 50g natto, after 14 days, find that the amount of the farnoquinone in the serum has certain increase, γ in the corresponding terrain-hydroxylated BMP amount also increases, because this albumen is the intermediate that bone forms, be very important material base therefore to osteoblastic osteogenesis function.With advancing age, farnoquinone amount in the body reduces gradually, and the sclerotin formation amount of osteocyte reduces, for the deficiency osteoclast that replenishes calcium utilizes calcium in the bone, thereby cause osteoporosis, so the farnoquinone in the natto there are prevention and treatment meaning for osteoporosis.
Isoflavonoid in the natto, as the substitute of female hormone also only with prevention and treatment osteoporosis, especially to menopausal women, the interior female hormone of body reduces gradually and causes that sclerotin absorbs again, bone density is lowered, isoflavones in the natto can improve the osteoporosis symptom, the osteogenic action of stimulating osteoblast, and the sclerotin that suppresses osteoclast absorbs again.
Contain the be absorbed with good facilitation of abundant γ-polyglutamic acid in addition in the natto cement for the calcium of human body.
γ-polyglutamic acid is a kind of big molecule that closes, and its inside has a large amount of negative electrical charges, but its Ca2+ ion and other material form insoluble compound.Make Ca2+ keep soluble state to reach 75%, therefore promote the sclerotin formation that is absorbed as of calcium to supply raw materials, certain effect is arranged for osteoporosis prevention and treatment.
After adding calcium in the sweat, increase the calcium concentration in this product, utilize the above-mentioned active factors that contains in the natto directly to promote the absorption of calcium.The organic acid that contains in the sweat of soybean in addition, flavonoids and Nattokinase and calcium reaction generate compound and help calcium in GI absorption, improve the utilization rate of calcium greatly, help replenishing the calcium of osteoporosis prevention and treatment and various crowds.
Contain natto powder of the present invention the health food scheme four: form by following each component by weight: natto powder 60-90%, aloe dry powder 10-40%.
Described aloe dry powder is to get Fresh leaf of aloe, and freeze drying is pulverized and got.
Mix above-mentioned natto powder and aloe dry powder and promptly get four of the health food scheme that contains natto powder.This health food is the health food that is used to improve gastrointestinal function, its function with cure mainly: have the effect of adjusting stomach and intestine, sterilization, defaecation, enhancing digestive function, be mainly used in constipation, enteritis, chronic gastritis crowd's health functional food.
Usage: every bag of 5g of a 1-2 bag, one day 2-3 time.
Bacillus natto in the natto has the effect of powerful adjustment stomach and intestine, contain 1,000,000,000 Bacillus nattos in the 1 gram natto, and its whole intestines effect is surprisingly big.Bacillus natto is not influenced by the strong acid of gastric juice, can arrive in the intestines by gastric juice smoothly, suppress the harmful bacterium in the enteron aisle, and to good growth and development environment of beneficial bacterium creation, harmful bacterium and virus in the enteron aisle are effectively killed, suppressed to the pyridinedicarboxylic acid that Bacillus natto produced, make intestinal flora reach a healthy dynamic equilibrium, strengthen the human consumption function, solve constipation, enteritis, diarrhoea problem rapidly, also the enteron aisle of your health.Eat natto every day, symptom will alleviate in 1～2 day, and 3 many weeks will be received beyond thought effect.
The function of the medication of aloe collection and Dietotherapy health is because of it has clearing away heat and loosing the bowels, desinsection treatment infantile malnutrition due to digestive disturbances or intestinalparasites, myogenic cures the wound and effect such as anti-inflammation.The traditional Chinese medical science thinks, aloe is cold in nature, bitter, go into the effect of liver, the heart, spleen, stomach, intestines warp, tool heat-clearing, defaecation, desinsection.Modern medicine study shows that the anthraquinone component that contains in the aloe has the enterocinesia of increasing, antibiotic, antiinflammation, and aloe polysaccharide has the raising body immunity, regulates body function, improves the effect of multiple chronic disease symptom.
Four experiments of adjusting the gastrointestinal health-care product functions of loosening bowel relieving constipation that contain the health food scheme of natto powder are observed
Method: experiment mice is in ig administration fasting 22 hours in the time of the 8th day, ig administration in the 9th day contains the medicine of the dosage of 50% burnt black ink, each mouse places the container of separation to observe separately, write down in time, 6 hours of the first grain row's melena of each mouse mouse defecation grain number, defecation proterties and ight soil and be infected with the anus situation, three groups of experiment small components, experiment I group is medicine small dose group 1.0g/kg every day for water, experiment II group, experiment II group is middle dosage group 2.5/kg every day, and experiment III group is the heavy dose of group of medicine 2.5/kg every day.
Four the health food that contains natto powder of table 7, scheme is to mouse first grain defecation time and the influence with interior defecation grain number in 6 hours
| Group | Dosage (g/kg) | Number of animals | The harmonic mean (min) of first grain row's melena time | The inverse (1/h) of first grain row's melena time (x+s) | Defecation grain number in the 6h (x ± s) |
| Water medicine medicine medicine | 0 1.0g/kg 2.5g/kg 5.01/kg | 10 10 10 10 | 362.30 229.0 168.54 127.39 | 0.166±0.12 0.262±0.106 0.241±0.179 0.462±0.29 | 1.7±1.49 2.67±1.0 2.38±2.07 3.56±1.59 |
The experimental result of table 7 shows that these health products have ease constipation and defecation, and has dose dependent.
Contain natto powder of the present invention the health food scheme five: mix by following each component and to form: natto powder 40-80g, aloe dry powder 5-20g, pollen pini 15-30, Flos puerariae lobatae extract 1-5g.Described aloe dry powder is to get Fresh leaf of aloe, and freeze drying is pulverized standby; Described pollen pini is to collect fresh pollen pini, uses 75% ethanol disinfection, drying for standby; Described Flos puerariae lobatae extract is that the FI puerariae water is extracted, and it is standby to be condensed into medicinal extract.
Contain natto powder of the present invention the health food scheme five are relieving alcoholisms, the health food of sobering up.Function with cure mainly: have anti-oxidant, strengthen immunity, promote cellular metabolism, the vigor that refresh oneself, inducing resuscitation increases brain cell.Can be used for relieving the effect of alcohol, control wine poison outbreak and relieving alcoholism, can improve drunk back wake up when coming various symptoms as, feel sick, headache, dizzy, weak etc.
Usage and consumption: a 1-2 bag, every bag of 5g, one day secondary.
The soybean saponin inhibition ethanol that contains in this prescription is in GI absorption and strengthen ethanol at GI first-pass effect, reduces the concentration of ethanol in blood.
Natto has the effect of decomposing alcohol composition, is regarded as dish that goes with wine always, and the effect of natto decomposing alcohol is just found recently.Table 8 is that health adult drinks the alcohol in the blood and the situation of change of aldehyde concentration after the whiskey that is equivalent to 30～65g.Peak when drinking the Bacillus natto zymotic fluid before drinking in the blood obviously reduces, and drops to 0.18mg/l with the alcohol concentration as a result of apparatus measures from 0.32mg/l after drinking.
Natto has the drunk effect of prevention, and the concentration of aldehyde has also had obvious variation as can be seen from Table 8.
The natto effective ingredient is proved by the animal experiment to the effect of aldehyde.When irritating a certain amount of acetaldehyde of stomach for the CDF1 mouse, because 24 hours later death rates of toxicity reach 65%; Even irritate the acetaldehyde of stomach with amount in advance the words of a spot of Bacillus natto zymotic fluid of mouse, 80% viral marketing survives, and promptly natto also can prevent alcoholism.
＜table 8-1〉irritate the alcohol concentration in the blood after the Bacillus natto zymotic fluid
| Time after the picked-up whiskey | ||||
| 1 hour | 2 hours | 3 hours | 4 hours | |
| The contrast bacterium | 20.0±3.4 | 16.3±3.4 | 9.6±3.4 | 5.5±2.4 |
| The Bacillus natto zymotic fluid | 15.6±3.9 | 14.2±3.7 | 9.0±3.6 | 5.2±3.2 |
| The blocking-up rate | 22.8％ | 12.9％ | 6.3％ | 5.5％ |
＜table 8-2〉irritate the aldehyde concentration in the blood after the Bacillus natto zymotic fluid
| Time after the picked-up whiskey | ||||
| 1 hour | 2 hours | 3 hours | 4 hours | |
| The contrast bacterium | 25.2±16.7 | 20.2±18.5 | 10.6±6.6 | 6.6±4.7 |
| The Bacillus natto zymotic fluid | 13.9±9.1 | 12.4±7.4 | 7.0±4.6 | 5.2±4.3 |
| The blocking-up rate | 44.8％ | 38.9％ | 34.0％ | 16.7％ |
Aloe and pollen pini have anti-inflammatory, detoxifcation, promotion cellular metabolism, sober up and deinebriating effect.FI puerariae always is used as the medicine that relieves the effect of alcohol.Whole prescription, demonstration is well sobered up, deinebriating effect.
The specific embodiment
Embodiment 1
Component is formed: natto powder 315g, flavone of hawthorn fruit 99g, garlic powder 22.5g, superfine silica gel powder 13.5g.After above-mentioned each component mixed according to quantity, carry out filling with No. 0 capsule and make 1000 capsules, every dress 0.45g.Usage and consumption: 3/time; 2 times/day.
The preparation method of above-mentioned natto powder wherein: the soya bean of getting after selected was soaked in water 10-24 hour; After it is fully steeped out, more than 100 ℃ and comprise under 100 ℃ of temperature boiling 20-60 minute; When the ripe beans after the boiling are cooled to 20-40 ℃, evenly spray into Bacillus natto liquid, cultivated 18-30 hour at temperature 35-45 ℃, relative humidity 50-90% condition bottom fermentation; Place in the 0-10 ℃ of refrigerating chamber refrigeration more than 24 hours the natto that ferments, produces more transparent haircuts; Take out, carry out freeze drying, pulverize, cross the 40-80 mesh sieve, promptly.
Embodiment 2
Component is formed: natto powder 337.5g, isoflavones 13.5g, Brazil mushroom crude polysaccharide 99g, and after above-mentioned each component mixed according to quantity, carry out filling with No. 0 capsule and make 1000 capsules, adorn 0.45g for every.Usage and consumption: 4/time; 2 times/day.
Wherein the preparation method of natto powder is with embodiment 1.
Embodiment 3
Component is formed: natto powder 100g, calcium lactate 20g, make 24 bags, every bag of 5g after above-mentioned each component mixed.Usage and consumption: 1 bag/time; 2 times/day.
Wherein the preparation method of natto powder is with embodiment 1.
Embodiment 4
Component is formed: natto powder 85g, fresh aloe dry powder 15g, make 20 bags, every bag of 5g after above-mentioned each component mixed.Usage and consumption: 1 bag/time; 2 times/day.
Wherein the preparation method of natto powder is with embodiment 1.
Embodiment 5
Component is formed: natto powder 77g, aloe dry powder 5g, pollen pini 15g, Flos puerariae lobatae extract 3g, the packaging bag of packing into after above-mentioned each component mixed, every bag of 5g.Usage and consumption: a 1-2 bag, one day secondary.
Wherein the preparation method of natto powder is with embodiment 1.
Claims (7)
1, a kind of preparation method of natto powder, be made up of the following step: the soya bean of getting after selected was soaked in water 10-24 hour; Under the temperature more than 100 ℃ boiling 20-60 minute then, when the ripe beans after the boiling are cooled to 20-40 ℃; Evenly spray into Bacillus natto liquid, cultivated 18-30 hour at temperature 35-45 ℃, relative humidity 50-90% condition bottom fermentation; Place in the 0-10 ℃ of refrigerating chamber refrigeration more than 24 hours the natto that ferments, produces more transparent haircuts; Take out, carry out freeze drying, pulverize, cross the 40-80 mesh sieve.
2, a kind of product-natto powder of making by the described preparation method of claim 1.
3, a kind of health food that contains the natto powder of claim 2 gained, form by following each component by weight:
Natto powder 60-80%, flavone of hawthorn fruit 15-25%, garlic powder 3-15%, superfine silica gel powder 2-5%.
4, a kind of health food that contains the natto powder of claim 2 gained, form by following each component by weight:
Natto powder 70-80%, isoflavones 3-5%, Brazil mushroom crude polysaccharide 17-25%.
5, a kind of health food that contains the natto powder of claim 2 gained, form by following each component by weight:
Natto powder 60-85%, calcium lactate 15-40%.
6, a kind of health food that contains the natto powder of claim 2 gained, form by following each component by weight:
Natto powder 60-90%, aloe dry powder 10-40%.
7, a kind of health food that contains the natto powder of claim 2 gained, form by following each component:
Natto powder 40-80g, aloe dry powder 5-20g, pollen pini 15-30g, Flos puerariae lobatae extract 1-5g.
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