CN102337206A - Enclosed bioreaction system - Google Patents
Enclosed bioreaction system Download PDFInfo
- Publication number
- CN102337206A CN102337206A CN2011103111801A CN201110311180A CN102337206A CN 102337206 A CN102337206 A CN 102337206A CN 2011103111801 A CN2011103111801 A CN 2011103111801A CN 201110311180 A CN201110311180 A CN 201110311180A CN 102337206 A CN102337206 A CN 102337206A
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- China
- Prior art keywords
- tfl
- carbon source
- pump housing
- pipeline group
- pump
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
Abstract
The invention discloses an enclosed bioreaction system, which consists of a power system and a reaction unit, wherein the power system comprises an energy source and a pump body which are connected; the reaction unit comprises a pipeline assembly, valves, a recovery tube, an air tube, a carbon source tube and a carbon source; one end of the pipeline assembly is connected with the outlet of the pump body, and the other end of the pipeline assembly is connected with the recovery tube; the valves are positioned on the top and the bottom of the recovery tube; one end of the recovery tube is connected with the pipeline assembly, and the other end of the recovery tube is connected with the pump body; the upper part of the recovery tube is provided with an exhaust port which is controlled to be opened or closed by a valve; the air tube is arranged in the recovery tube; one end of the air tube is positioned outside the recovery tube and is provided with a valve, and the other end of the air tube is positioned between the bottom of the recovery tube and the pump body; and one end of the carbon source tube is connected with the carbon source, and the other end of the carbon source tube is positioned between the bottom of the recovery tube and the pump body. The whole set of system is low in energy consumption, simple in structure, and easy to operate; the utilization rate of air and the carbon source is improved; massive equipment is saved, and the cost is saved.
Description
Technical field
The invention belongs to technical field of bioengineering, be specifically related to the closed bio-reaction system in a kind of microorganism culturing.
Background technology
Mikrobe is most important a kind of biology on the earth, is important in a biosphere integral part.The development and utilization mikrobe can provide amounts of protein, VITAMINs, Serlabo, glucide, pigment, mineral substance, polysaccharide, fat and unsaturated fatty acids etc. for human society; And can cultivate throughout the year; Output is tens times even the hundreds of times of food crop, and economic benefit is thousands of times of cash crop.
Microbe species is various, and different varieties has different requirement to survival environment, and the development and utilization mikrobe must reach single pure culture of planting, and whole process is necessary for closed, stops fungi, bacterium, dust, protozoic influence.Culturing micro-organisms adopts closed column tank body reactor drum at present; Several centimetres of this reactor drum tank body diameters are to hundreds of centimetre; Volume is several tons to tens tons, and body wall is thicker, and temperature is just transmitted through the body wall interlayer; Control but often need consume mass energy, and can not accomplish instant effectively regulation and control; Be provided with large-scale spiral blade and motor in the middle of the tank body, reactor operation need consume a large amount of electric energy; The culture soln liquid level is darker in the tank body; Provide oxygen and carbonic acid gas just must supply with to mikrobe, and two kinds of gases solubleness in nutrient solution is low through specific equipment compression back, and long-pending little with the nutrient solution contact level; Duration of contact is short; A large amount of gases just are not utilized discharges, and just instability, carbon source lack, dissolved oxygen is not enough to cause the pH value of solution value, and then cause that microorganism culturing efficient is low.
Therefore,, expend few energy, increase the utilization ratio of gas and carbon source, improve microorganism culturing efficient, become the key core technical problem of mikrobe development and use keeping how effectively coming regulating and controlling temperature immediately under the constant situation of volume of culture.
Summary of the invention
The objective of the invention is problem, the closed bio-reaction system in a kind of new microbial cultivation is provided to above-mentioned existence.It can effectively solve air and the low problem of utilization of carbon source rate, for microorganism growth provides best culture environment.
The present invention addresses the above problem the technical scheme that is adopted:
This system is made up of power system and reaction member, and power system comprises the energy and the pump housing, and the energy is connected with the pump housing; Reaction member comprises pipeline group, valve, TFL, blast main, carbon source pipe, carbon source; Pipeline group one end is connected with pump housing outlet, and the other end is connected with TFL, and valve lays respectively at TFL top and TFL bottom; TFL one end is connected with pipeline group, and the other end is connected with the pump housing, and top is provided with venting port; Blast main is arranged in the TFL, and wherein an end is positioned at the TFL outside, and is designed with valve, and the other end is positioned between the TFL bottom and the pump housing, and carbon source Guan Yiduan links to each other with carbon source, and the other end is positioned between the TFL bottom and the pump housing.
Described TFL is that material transparent constitutes, and diameter and pipeline group are isometrical, the vertical placement.
Described pipeline group caliber is DN (internal diameter) 20～DN110, and material can be made up of Vilaterm, SE, Vestolen PP 7052, glass or stainless steel etc., can be transparent, translucent or opaque.
Described pipeline group can be vertically, inclination, horizontal positioned, is made up of many pipelines polyphones and parallel connection.
The said pump housing can be impeller pump, reciprocation pump, toothed gear pump, volute pump etc.
Said blast main and carbon source pipe diameter are DN3～DN12, and material can be formations such as silica gel, Vilaterm or SE.
Described carbon source can be plant gas, vehicle exhaust, oil liquefied gas, bavin coal waste gas, biogas or organism etc.
The present invention and prior art relatively have the following advantages:
1, blade and motor need be installed in the TFL, caliber is less relatively, and liquid is taken away whole cultures in the pipeline, and culture is difficult for being deposited in tube wall.
2, total system easy control of temperature can be regulated immediately, and the pipeline group diameter is less, and it is fast to conduct heat, and on tube wall, sprays hot water or frozen water, and temperature just can be controlled in the scope of regulation.
3, the carbon source of this system's use can be liquid, gaseous state, and breaking away from must be the traditional method that could use after the liquefaction of gases.
4, air and carbon supplied mode are utilized venturi principle, reclaim liquid in pipe after pump housing speed-raising, produce negative pressure in the TFL, and air and carbon source just are inhaled in the liquid, break away from the method that needs pump and press device to come malleation to supply with originally.
5, air and carbon source are through behind the pump housing; Pressure increases, and solubleness increases, and in pipeline group, follows cultivation liquid and circulates many times together; Contact area and touch opportunity have been increased; Provide the competent time to absorb to mikrobe, can reach 100% utilization, to the greatest extent the pH value of nutrient solution is maintained in the stable range.
6, total system is simple in structure, and floor space is little, can install according to actual landform, as on the car, factory, kitchen, really reach zero release.
7, the total system power consumption is little, and unique power is the pump housing, and the energy can be wind energy, sun power, chemical energy, electric energy or mechanical energy, can directly or indirectly to the pump housing energy be provided.
Description of drawings
Fig. 1 is a closed bio-reaction system structural representation of the present invention.
Among the figure, 1-vent valve, 2-air pipe valve, 3-blast main, 4-carbon source pipe, 5-carbon source, 6-TFL, 7-recovery pipe valve, the 8-pump housing, 9-pipeline group.
Embodiment
As shown in Figure 1, concrete structure of the present invention is following:
Closed bio-reaction system of the present invention is made up of power system and reaction member, and power system comprises the energy and the pump housing 8, and the energy is connected with the pump housing 8.Reaction member comprises pipeline group 9, vent valve 1, air pipe valve 2 and recovery pipe valve 7, TFL 6, blast main 3 and carbon source pipe 4; Pipeline group 9 one ends are connected with the pump housing 8 outlets; The other end is connected with TFL 6; Vent valve 1 is positioned at TFL 6 tops, and air pipe valve 2 is positioned on the blast main 3, recovery pipe valve 7 is positioned at TFL 6 bottoms; TFL 6 one ends are connected with pipeline group 9, and the other end is connected with the pump housing 8; Blast main 3 is arranged in the TFL 6, and wherein an end is positioned at TFL 6 outsides, and the other end is positioned at 8 of TFL 6 bottoms and the pump housings, and carbon source pipe 4 one ends link to each other with carbon source 5, and the other end is positioned at 8 of TFL 6 bottoms and the pump housings.
Described TFL is that material transparent constitutes, and diameter and pipeline group are isometrical, the vertical placement.
Described pipeline group caliber is DN20～DN110, and material can be made up of Vilaterm, SE, Vestolen PP 7052, glass or stainless steel etc., can be transparent, translucent or opaque.
Described pipeline group can be vertically, inclination, horizontal positioned, is made up of many pipelines polyphones and parallel connection.
The said pump housing can be impeller pump, reciprocation pump, toothed gear pump, volute pump etc.
Said blast main and carbon source pipe diameter are DN3～DN12, and material can be formations such as silica gel, Vilaterm or SE.
Described carbon source can be plant gas, vehicle exhaust, oil liquefied gas, bavin coal waste gas, biogas or organism etc.
Installation method:
0.1 the closed bio-reaction system of ton is chosen on the level land and installs.
At first, pipe laying group 9 on the level land, pipeline group 9 is by 40 of the long Glass tubings of 2m (DN40); Per 10 is a polyphone unit, is installed as 4 parallel connections then, and TFL 6 is positioned at pipeline group 9 one sides; TFL 6 is vertically installed, and carbon source 5 is selected plant gas, and the energy 5 uses electric energy; The pump housing 8 uses impeller pump, and other installs related equipment successively according to Fig. 1 pattern.
After installing, carry out thorough disinfection and handle, disinfection way is traditional chemical treatment, is sterilizing repeatedly through sterilizing agents such as alcohol, Youxiaolin, potassium permanganate, ozone, dioxide peroxide, with the sterilized water of sterilizing total system is cleaned up at last.
Utilize the detailed process of above-mentioned reactive system culturing micro-organisms (is representative with the haematococcus pulvialis) following:
(1) open vent valve 1, inject Haematocoocus Pluvialls algae kind toward 6 li of TFLs, volume is 10kg, again supplemented medium 90kg.
(2) start power supply, make the pump housing 8 entrys into service, liquid begins the cycles back and forth running in the whole system.
(3) open pressure lock 3 and carbon source 5, utilize the Venturi principle of work, air and carbonic acid gas are inhaled into 9 li of pipeline group; Air and carbonic acid gas are broken into trickle bubble through behind the pump housing, and contact area increases, and pressure also increases; Solubleness increases considerably; Through the circulation of 80m, carbonic acid gas has all been absorbed by Haematocoocus Pluvialls in nutrient solution, and the oxygen that air that has not absorbed and photosynthesis produce is discharged through vent valve 1.
(4) through measuring pH value and the dissolved oxygen height of cultivating liquid, regulate and control pressure lock 2 immediately and supply with carbon source 5.
(5) in system's operational process,, maintain the temperature in the certain limit (20～25 ℃) through spraying hot water or frozen water to reactor unit.
(6) after cultivation after a while, the haematococcus pluvialis cell that a whole set of reactor drum is cultivated reaches the density of results, stops to cultivate, and powered-down is opened recovery pipe valve 7 and collected.
(7) after collection finishes,, wash away residual disinfectancy liquid with sterilized water more at last, can restart the cultivation of next round Haematocoocus Pluvialls then in whole system, adding a certain amount of thimerosal sterilization.
The result who implements shows closed bio-reaction system of the present invention: it is low to consume energy, and whole system is simple in structure, easily operation; Utilize the Venturi principle of work to suck air and carbon source, through the stirring of pump housing thorough mixing, supercharging, air and utilization of carbon source rate improve again; Save a large amount of equipment, practiced thrift cost, for microorganism growth provides best suitable condition; Effectively shorten incubation time, this system can be used for continuous or semicontinuous microorganism culturing, supports for the exploitation Microbial resources provide actual techniques.
Claims (7)
1. a closed bio-reaction system is characterized in that this system is made up of power system and reaction member, and power system comprises the energy and the pump housing, and the energy is connected with the pump housing; Reaction member comprises pipeline group, valve, TFL, blast main, carbon source pipe, carbon source, and pipeline group one end is connected with pump housing outlet, and the other end is connected with TFL, and valve is positioned at TFL top and TFL bottom; TFL one end is connected with pipeline group, and the other end is connected with the pump housing, the upper design venting port; Blast main is arranged in the TFL, and wherein an end is positioned at the TFL outside, and is designed with valve, and the other end is positioned between the TFL bottom and the pump housing, and carbon source end Guan Yiduan links to each other with carbon source, and the other end is positioned between the TFL bottom and the pump housing.
2. closed bio-reaction system according to claim 1 is characterized in that: TFL is a material transparent, and diameter and pipeline group are isometrical, the vertical placement.
3. closed bio-reaction system according to claim 1 is characterized in that: the pipeline group caliber is DN20～DN110, and material is Vilaterm or SE or Vestolen PP 7052 or glass or stainless steel.
4. closed bio-reaction system according to claim 1 is characterized in that: pipeline group is vertical or inclination or horizontal positioned, is made up of many pipelines polyphones or parallel connection.
5. closed bio-reaction system according to claim 1 is characterized in that: the pump housing is impeller pump or reciprocation pump or toothed gear pump or volute pump.
6. closed bio-reaction system according to claim 1 is characterized in that: blast main and carbon source pipe diameter are DN3～DN12, and material is in silica gel or Vilaterm or the SE.
7. closed bio-reaction system according to claim 1 is characterized in that: carbon source is one or more in plant gas, vehicle exhaust, oil liquefied gas, bavin coal waste gas, biogas, the organism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103111801A CN102337206A (en) | 2011-10-14 | 2011-10-14 | Enclosed bioreaction system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103111801A CN102337206A (en) | 2011-10-14 | 2011-10-14 | Enclosed bioreaction system |
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| CN102337206A true CN102337206A (en) | 2012-02-01 |
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| CN2011103111801A Pending CN102337206A (en) | 2011-10-14 | 2011-10-14 | Enclosed bioreaction system |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN102061256A (en) * | 2009-11-11 | 2011-05-18 | 陈志威 | Compound culture tube, preparation method thereof and enclosed algae culture system |
-
2011
- 2011-10-14 CN CN2011103111801A patent/CN102337206A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN102061256A (en) * | 2009-11-11 | 2011-05-18 | 陈志威 | Compound culture tube, preparation method thereof and enclosed algae culture system |
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Application publication date: 20120201 |