CN102321719A - Industrial production method for preparing collagen from fish scale by enzyme method - Google Patents
Industrial production method for preparing collagen from fish scale by enzyme method Download PDFInfo
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- CN102321719A CN102321719A CN201110298454A CN201110298454A CN102321719A CN 102321719 A CN102321719 A CN 102321719A CN 201110298454 A CN201110298454 A CN 201110298454A CN 201110298454 A CN201110298454 A CN 201110298454A CN 102321719 A CN102321719 A CN 102321719A
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- fish scale
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Abstract
The invention relates to the biological field and particularly discloses an industrial production method for preparing collagen from fish scale by an enzyme method. The industrial production method for preparing the collagen from the fish scale by the enzyme method is characterized by comprising the following steps of: deliming, performing size mixing, performing enzymolysis, deactivating an enzyme, decolorizing, filtering for the first time by using a ceramic membrane, deodorizing, filtering for the second time by using the ceramic membrane, performing ion exchange, performing vacuum concentration, sterilizing and spray-drying. The industrial production method for preparing the collagen from the fish scale by the enzyme method has the advantages that: the process is easy to operate, the enzymolysis is complete, intermediate products can be filtered easily, the production cycle is short, and the industrialized production can be realized easily; a produced collagen product is white, has no fishy smell, and has good mouthfeel and high purity, the content of proteins is over 85 percent, and the collagen product can be directly eaten when used as a protein nutritional supplement and can also be used as a functional ingredient of clinical nutrition products, health-care foods, sports foods and cosmetics; and a novel way is found for improving the value of fish scale products and producing high-value products.
Description
(1) technical field
The present invention relates to biological field, enzyme process is produced the industrial process of collagen protein in particularly a kind of fish scale.
(2) background technology
Collagen protein is a kind of extracellular protein; It is to walk to be found to twist into spiral fibrous protein by 3 peptide chains by SINOMOS/A29 in biomedicine beauty treatment forefront; Collagen protein is the rich in protein of people's in-vivo content; Account for more than 30% of whole body gross protein, collagen protein is rich in amino acid such as glycocoll that human body needs, proline(Pro), oxyproline.Collagen protein is a most important component in the extracellular matrix.3 kilograms of collagen proteins are arranged in the adult health approximately; Mainly be present in human body skin, bone, eyes, tooth, tendon, internal organ positions such as (comprising the heart, stomach, intestines, blood vessel); Its function is to keep the form and the structure of skin and histoorgan, also is the important source material material of repairing each damaged tissue.In the human body skin composition, form by collagen protein by 70%.When collagen protein was not enough, not only skin and bone can go wrong, and also can have a negative impact to internal organs.That is to say that collagen protein is to keep the indispensable important component of health normal activity.Simultaneously also be to make health keep young, prevent the aged material.In addition, all right preventing disease of collagen protein is improved physique, and is all helpful to beauty treatment and health.The existing method of producing collagen protein with the direct enzymolysis of fish scale, difficulty is big, ash content is many, be difficult for to filter, to obtain the albumen rate low.
(3) summary of the invention
The present invention is in order to remedy the deficiency of prior art, provides enzyme process in a kind of technology fish scale simple, with short production cycle to produce the industrial process of collagen protein.
The present invention realizes through following technical scheme:
Enzyme process is produced the industrial process of collagen protein in a kind of fish scale, it is characterized in that: comprise the steps:
(1) deliming is handled: drop into 1 ton of fish scale, add the hydrochloric acid soln 20m of 0.4mol/L
3Immersion treatment 2 hours leaves standstill, and suction filtration falls hydrochloric acid soln, again water suction filtration four times repeatedly;
(2) size mixing: with the fish scale handled well use vaal water size mixing to the slurries massfraction be 8%;
(3) enzymolysis: the fish scale solution that will size mixing is heated to 60 ℃, transfers PH to 5.9-6.1 with acid solution, adds the Bacillus subtillis proteolytic enzyme of fish scale gross weight 1% and the papoid of fish scale gross weight 1%, and enzymolysis time is 2 hours;
(4) enzyme that goes out: through plate-type heat exchanger, temperature is 115-120 ℃ with enzymolysis solution;
(5) decolouring: the feed liquid behind the enzyme that will go out adds the gac of fish scale gross weight 2%, and 90 ℃ of temperature were soaked 30 minutes;
(6) ceramic membrane filter for the first time: the feed liquid of soaking after 30 minutes is carried out membrane filtration;
(7) take off raw meat: feed liquid is cooled to 35 ℃, adds the yeast of fish scale gross weight 2%, reacted 1.5 hours;
(8) ceramic membrane filter for the second time: the reacted feed liquid of step (7) is crossed ceramic membrane once more;
(9) IX: with speed is 2m
3/ h carries out the zwitterion exchange;
(10) vacuum concentration: under-0.07Mpa, 70 ℃ vacuum condition, feed liquid is concentrated into solid content 20-25% with the triple effect concentrating pan;
(11) sterilization: sterilising temp is 130-135 ℃;
(12) spraying drying: adopt spray-drying tower dry, inlet temperature is 170 ℃, and temperature out is 80 ℃, promptly gets collagen protein after the spraying drying.
The ceramic membrane aperture is 0.2 micron in step (6) and the step (8).
Enzyme process is produced the beneficial effect of the industrial process of collagen protein and is in the fish scale of the present invention: technological operation is simple, and thorough enzymolysis is prone to filter; With short production cycle, be prone to realize suitability for industrialized production, the collagen protein product of being produced white, no fishy smell; Mouthfeel is good; Purity is high, and protein contnt can be used as the nutrient protein supplement and directly eats more than 85%; Also can be used as the function batching of clinical nutrition product, protective foods, motion food and makeup, for the product that improves fish scale value of the product, production high value has found new approach.
(4) embodiment
Enzyme process is produced the industrial process of collagen protein in this fish scale, comprises the steps:
(1) deliming is handled: drop into 1 ton of fish scale, add the hydrochloric acid soln 20m of 0.4mol/L
3Immersion treatment 2 hours leaves standstill, and suction filtration falls hydrochloric acid soln, again water suction filtration four times repeatedly;
(2) size mixing: with the fish scale handled well use vaal water size mixing to the slurries massfraction be 8%;
(3) enzymolysis: the fish scale solution that will size mixing is heated to 60 ℃, transfers PH to 5.9-6.1 with acid solution, adds 10 kilograms Bacillus subtillis proteolytic enzyme and 10 kilograms papoid, and enzymolysis time is 2 hours;
(4) enzyme that goes out: through plate-type heat exchanger, temperature is 115-120 ℃ with enzymolysis solution;
(5) decolouring: the feed liquid behind the enzyme that will go out adds 20 kilograms gac, and 90 ℃ of temperature were soaked 30 minutes;
(6) ceramic membrane filter for the first time: the feed liquid of soaking after 30 minutes is carried out membrane filtration, and the ceramic membrane aperture is 0.2 micron;
(7) take off raw meat: feed liquid is cooled to 35 ℃, adds 20 kilograms yeast, (it is preferable with feed liquid the yeast soaking fermentation to be added effect in 20 minutes more earlier before adding) reacted 1.5 hours;
(8) ceramic membrane filter for the second time: the reacted feed liquid of step (7) is crossed ceramic membrane once more, and the ceramic membrane aperture is 0.2 micron;
(9) IX: with speed is 2m
3/ h carries out the zwitterion exchange, and resin cation(R.C.) is 732 types (Tianjin Nankai University), and resin anion(R.A) is gel B214 type (an Xi'an Ke An company);
(10) vacuum concentration: under-0.07Mpa, 70 ℃ vacuum condition, feed liquid is concentrated into solid content 20-25% with the triple effect concentrating pan;
(11) sterilization: sterilising temp is 130-135 ℃;
(12) spraying drying: adopt spray-drying tower dry, inlet temperature is 170 ℃, and temperature out is 80 ℃, promptly gets collagen protein after the spraying drying.
Preferred version of the present invention is Hainan tilapia fish scale, and other fish fish scales also can.
Claims (2)
1. enzyme process is produced the industrial process of collagen protein in the fish scale, it is characterized in that: comprise the steps:
(1) deliming is handled: drop into 1 ton of fish scale, add the hydrochloric acid soln 20m of 0.4mol/L
3Immersion treatment 2 hours leaves standstill, and suction filtration falls hydrochloric acid soln, again water suction filtration four times repeatedly;
(2) size mixing: with the fish scale handled well use vaal water size mixing to the slurries massfraction be 8%;
(3) enzymolysis: the fish scale solution that will size mixing is heated to 60 ℃, transfers PH to 5.9-6.1 with acid solution, adds the Bacillus subtillis proteolytic enzyme of fish scale gross weight 1% and the papoid of fish scale gross weight 1%, and enzymolysis time is 2 hours;
(4) enzyme that goes out: through plate-type heat exchanger, temperature is 115-120 ℃ with enzymolysis solution;
(5) decolouring: the feed liquid behind the enzyme that will go out adds the gac of fish scale gross weight 2%, and 90 ℃ of temperature were soaked 30 minutes;
(6) ceramic membrane filter for the first time: the feed liquid of soaking after 30 minutes is carried out membrane filtration;
(7) take off raw meat: feed liquid is cooled to 35 ℃, adds the yeast of fish scale gross weight 2%, reacted 1.5 hours;
(8) ceramic membrane filter for the second time: the reacted feed liquid of step (7) is crossed ceramic membrane once more;
(9) IX: with speed is 2m
3/ h carries out the zwitterion exchange;
(10) vacuum concentration: under-0.07Mpa, 70 ℃ vacuum condition, feed liquid is concentrated into solid content 20-25% with the triple effect concentrating pan;
(11) sterilization: sterilising temp is 130-135 ℃;
(12) spraying drying: adopt spray-drying tower dry, inlet temperature is 170 ℃, and temperature out is 80 ℃, promptly gets collagen protein after the spraying drying.
2. enzyme process is produced the industrial process of collagen protein in the fish scale according to claim 1, it is characterized in that: the ceramic membrane aperture is 0.2 micron in step (6) and the step (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201110298454A CN102321719A (en) | 2011-09-28 | 2011-09-28 | Industrial production method for preparing collagen from fish scale by enzyme method |
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| CN201110298454A CN102321719A (en) | 2011-09-28 | 2011-09-28 | Industrial production method for preparing collagen from fish scale by enzyme method |
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| CN102321719A true CN102321719A (en) | 2012-01-18 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613383A (en) * | 2012-04-24 | 2012-08-01 | 天津春宇食品配料有限公司 | Fish scale protein enzymolysis liquid and preparation method thereof |
| CN103082080A (en) * | 2013-02-07 | 2013-05-08 | 广东海洋大学 | Fish skin and fish scale protein enzymolysis powder with function of relieving fatigue |
| CN104726527A (en) * | 2015-03-24 | 2015-06-24 | 宁德市夏威食品有限公司 | Preparation technology for producing collagen through enzymolysis of fish scales |
| CN104824376A (en) * | 2015-05-06 | 2015-08-12 | 宁德市夏威食品有限公司 | Production technology of complex-agglomerated micro-capsule fermented aquatic feed additive and additive |
| CN106519021A (en) * | 2016-12-20 | 2017-03-22 | 广西品位生物科技有限公司 | Preparation method of cod collagen |
| WO2017209587A1 (en) | 2016-06-01 | 2017-12-07 | Institut National De Recherche Halieutique | Method for the extraction and purification of native marine collagen from sardina pilchardus sardine scales |
| CN109793672A (en) * | 2018-12-24 | 2019-05-24 | 中健(广州)生物医药研究院有限公司 | Application of the fish oligopeptide in cosmetics |
| CN113135992A (en) * | 2021-05-11 | 2021-07-20 | 首曜(江苏)生物科技有限公司 | Preparation method of fish scale collagen peptide |
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| CN1775950A (en) * | 2005-10-19 | 2006-05-24 | 由守谊 | Fishscale collagen production process |
| CN101709319A (en) * | 2009-11-20 | 2010-05-19 | 华南理工大学 | Method for preparing fish skin and scale collagen peptide |
| CN101724677A (en) * | 2009-12-15 | 2010-06-09 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting collagen polypeptide and hydroxyapatite in fish scales by cooking hot extrusion |
| CN101812496A (en) * | 2010-06-07 | 2010-08-25 | 福州大学 | Preparation method for high purity fish scale collagen |
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| CN101709319A (en) * | 2009-11-20 | 2010-05-19 | 华南理工大学 | Method for preparing fish skin and scale collagen peptide |
| CN101724677A (en) * | 2009-12-15 | 2010-06-09 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting collagen polypeptide and hydroxyapatite in fish scales by cooking hot extrusion |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613383A (en) * | 2012-04-24 | 2012-08-01 | 天津春宇食品配料有限公司 | Fish scale protein enzymolysis liquid and preparation method thereof |
| CN102613383B (en) * | 2012-04-24 | 2015-04-15 | 天津春宇食品配料有限公司 | Fish scale protein enzymolysis liquid and preparation method thereof |
| CN103082080A (en) * | 2013-02-07 | 2013-05-08 | 广东海洋大学 | Fish skin and fish scale protein enzymolysis powder with function of relieving fatigue |
| CN104726527A (en) * | 2015-03-24 | 2015-06-24 | 宁德市夏威食品有限公司 | Preparation technology for producing collagen through enzymolysis of fish scales |
| CN104824376A (en) * | 2015-05-06 | 2015-08-12 | 宁德市夏威食品有限公司 | Production technology of complex-agglomerated micro-capsule fermented aquatic feed additive and additive |
| WO2017209587A1 (en) | 2016-06-01 | 2017-12-07 | Institut National De Recherche Halieutique | Method for the extraction and purification of native marine collagen from sardina pilchardus sardine scales |
| CN106519021A (en) * | 2016-12-20 | 2017-03-22 | 广西品位生物科技有限公司 | Preparation method of cod collagen |
| CN109793672A (en) * | 2018-12-24 | 2019-05-24 | 中健(广州)生物医药研究院有限公司 | Application of the fish oligopeptide in cosmetics |
| CN113135992A (en) * | 2021-05-11 | 2021-07-20 | 首曜(江苏)生物科技有限公司 | Preparation method of fish scale collagen peptide |
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Inventor after: Zhang Jiuxun Inventor after: Li Lei Inventor after: Zhang Xuejun Inventor after: Zhang Guiwen Inventor after: Zhang Ping Inventor before: Zhang Jiuxun |
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Free format text: CORRECT: INVENTOR; FROM: ZHANG JIUXUN TO: ZHANG JIUXUN LI LEI ZHANG XUEJUN ZHANG GUIWEN ZHANG PING |
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Application publication date: 20120118 |