CN102318846B - Processing method of jack-fruit - Google Patents
Processing method of jack-fruit Download PDFInfo
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- CN102318846B CN102318846B CN201110150821XA CN201110150821A CN102318846B CN 102318846 B CN102318846 B CN 102318846B CN 201110150821X A CN201110150821X A CN 201110150821XA CN 201110150821 A CN201110150821 A CN 201110150821A CN 102318846 B CN102318846 B CN 102318846B
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- enzymolysis
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- jackfruit
- stoste
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Abstract
The invention relates to the processing field of food, and in particular to a processing method of jack-fruit. Enzymatic hydrolysis and subsequent purification procedures are undertaken on seeds and bud meat of jack-fruit, so contents of amylose, polypeptide, aqueous soluble starch, cellulose, vitamins and minerals in the jack-fruit extracts can be improved.
Description
Technical field
The present invention relates to food processing field, particularly a kind of processing method of jack-fruit.
Background technology
Jackfruit is typical tropical fruit (tree), originates in India, and the tropic countries such as Sri Lanka, Burma, Indonesia all have cultivation at present.China's cultivation jackfruit is the history in existing more than 1,000 year so far, and the torrid zone, the South Subtropical Area of China of existing Guangdong, Guangxi, Hainan, Yunnan, Fujian and South Sichuan all have cultivation, and Hainan Province's plantation at most.
Modern medicine study confirms, contains abundant carbohydrate, protein, B family vitamin (B in the jackfruit
1, B
2, B
6), vitamin C, mineral matter, fatty wet goods, the normal physiological function of keeping body is had important function.The hydrolysis of energy intensive aspect inner fibrin after the edible jackfruit, can will block in tissue and endovascular fibrin and dissolution of blood clot, thereby improve local blood, body fluid circulation, make inflammation and absorption of edema, disappear, cerebral thrombus and the caused disease of other thrombus are also had certain auxiliary therapeutic action.
Pulp contains total reducing sugar 20.5～21.7%, protein 1.05～1.72%, fat 0.6%, carbohydrate 23.4%, ash content 0.5%, cellulose 1.8% in the jackfruit fruit; Seed carbohydrate containing 38.4%, protein 6.6%, fat 0.4%, cellulose 1.5%.But, at present to the exploitation of jackfruit bud meat only be by to the drying of jackfruit bud meat, toast and make luxuriant sliced meat or sugar is made preserved fruit, exploitation to the jackfruit seed just makes starch through the method for physics, process is easily destroyed the nutritional labeling in jackfruit bud meat and the seed, further affects the recovery rate of each nutritional labeling.
Summary of the invention
In view of this, the invention provides a kind of processing method of jack-fruit.The method is carried out enzymolysis and subsequent purification step by seed and the luxuriant meat to jackfruit, has improved the content of the nutritional labelings such as polysaccharide, polypeptide in the jackfruit extract.
In order to realize the foregoing invention purpose, the invention provides following technical scheme:
The invention provides a kind of processing method of jack-fruit, comprising:
Step 1: the seed of jackfruit is soaked 30min with 0.5～1% sodium bicarbonate solution, remove epidermis behind the heating and cooling, be mixed to get raw material with the luxuriant meat of jackfruit after cleaning oven dry, in raw material, add entry and make stoste;
Step 2: adding alkali in described stoste, to make the pH value of described stoste be 8.3～8.7, under 43～47 ℃, adds 1.0～10.0 ten thousand IU pancreatin in every kg raw material, and enzymolysis 2～6h obtains the first enzymolysis liquid;
Step 3: adding acid in described the first enzymolysis liquid, to make the pH value of described the first enzymolysis liquid be 3.8～4.2, under 43～47 ℃, add in the AMS, pectase, cellulase, carbohydrase, papain of 1.0～20.0 ten thousand IU one or more in every kg raw material, enzymolysis 2～6h obtains the second enzymolysis liquid;
Step 4: described the second enzymolysis liquid is made the jackfruit extract through the separation of DEAE chromatographic column, ultrafiltration.
Pancreatin, be through extract, refining, the composite a kind of complex enzyme that forms, contain protease, lipase, amylase etc., have the abilities such as stronger decomposing protein and fat, be widely used in food, medical treatment, brewage, the industries such as silk, process hides.Processing method of jack-fruit provided by the invention, in the pancreatin of selection, in enzyme activity (IU), trypsase: amylopsin: pancreatic lipase=6: 70: 40.Preferably, in the method provided by the invention, in every 1g pancreatin, tryptic enzyme activity is 600～800IU, and the enzyme activity of amylopsin is 7000～9000IU, and the enzyme activity of pancreatic lipase is 4000～6000IU.
AMS, numbering: EC 3.2.1.1, diastatic a kind of, be endo-amylase, catalysis random hydrolysis α-Isosorbide-5-Nitrae-glycosidic bond produces maltose, maltotriose and schardinger dextrin.Wherein, fungal alpha-amylase is a kind of AMS that is produced by the aspergillus microbial fermentation.Different from bacterialα-amylase is that the optimum temperature of fungal alpha-amylase is about 40～55 ℃, surpasses 60 ℃ of beginning inactivations; The maltose that its amylatic product mainly is high-load and some compound sugar and a small amount of glucose.And bacterialα-amylase optimum temperature high (70～80 ℃ of mesophilicα-diastases, high temperature resistant AMS are 95～105 ℃), amylatic primary product is dextrin.Therefore, bacterialα-amylase can only be used for fermentation industry, and fungal alpha-amylase then is widely used in the production of starch syrup, compound sugar, beer, bakery, Flour product etc.In the processing method of jack-fruit provided by the invention, select fungal alpha-amylase to carry out enzymolysis.
Pectase, a multienzyme complex of decompose pectin generally includes protopectinase, pectinesterase hydrolase, pectinesterase, by their synergy pectic substance is decomposed fully.Natural pectic substance changes into the pectin of water dissolvable under the protopectinase effect; Pectin is removed the methyl esters group by the catalysis of pectin methyl esters hydrolase, generates pectic acid; Pectic acid generates galacturonic acid through polygalacturonase class and the degraded of pectate lyase class.Pectase can be got through fermenting refining by aspergillus niger (Aspergillus niger), and decompose pectin is had good effect.Action pH value is: 2.5～6.0, and Optimun pH is: 3.5～4.5.
Cellulase is a kind of complex enzyme, mainly is comprised of circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase etc., also has the Xylanase activity of very high vigor.The unformed area of the random cutting fibre element of endoglucanase polysaccharide chain inside produces the oligosaccharides of different length and the end of new chain.Exoglucanase acts on the end of the fibrination sugar chain of these reproducibilities and irreducibility, discharges glucose or cellobiose.Beta-glucosidase can be decomposed into glucose with cellobiose, cellotriose and other low molecule cellodextrins.
Carbohydrase claims again glucoamylase, formal name used at school is α-1,4-glucose hydrolysis enzyme (α-1,4-Glucan glucohydrolace), can produce glucose to starch from non reducing end hydrolyzing alpha-Isosorbide-5-Nitrae glucoside bond, also slow hydrolyzing alpha-1,6 glucoside bonds are converted into glucose.The Substratspezifitaet of carbohydrase is lower, and it also can slowly cut α-1,6 except cutting α-Isosorbide-5-Nitrae key from the irreducibility end of starch chain.Therefore, what it can be very fast downcuts grape unit to amylose successively from the irreducibility end, cuts apart running into 1,6 key, first α-1,6 key is cut apart, and α-Isosorbide-5-Nitrae key is cut apart again, thereby is made amylopectin be hydrolyzed into glucose.Carbohydrase increases with temperature rising vigor, surpasses 65 ℃ and sharply descends with temperature rising vigor again, and optimum temperature is 60～62 ℃, optimum pH 4.0～4.5.
Papain, be called for short papain, claim again Fructus Chaenomelis ferment, being a kind of proteolytic enzyme that all can decomposing protein under acid, neutral, alkaline environment, antibody molecule can being hydrolyzed to 3 fragments, is a kind of low specific proteins hydrolase that contains in the papaya, the activated centre contains cysteine, belong to thiol protease, have wider substrate specificity, act on the peptide bond that L-arginine in the protein, 1B, glycine and Cit residue participate in formation; Papain belongs to endopeptidase, can cut the polypeptide class that whole-egg protein matter intramolecule peptide chain-CO-NH-generates molecular weight.The mechanism of the shearing peptide bond of papain comprises: Cys-25 deprotonation under the His-159 effect, and Asn-158 can help the putting of imidazole ring of His-159, so that deprotonation can occur; Then the carbonyl carbon on the Cys-25 nucleophillic attack peptide main chain, and covalently bound formation acyl group-enzyme intermediate with it; Removal of acylation occurs in then enzyme and hydrone effect, and discharges the carbonyl end of peptide chain.Water-soluble and the glycerine of papain, the aqueous solution is colourless or faint yellow, sometimes is creamy white; Be dissolved in hardly the organic solvents such as ethanol, chloroform and ether; The most suitable pH value is 5.7 (general 3～9.5 all can), effect is also arranged, isoelectric point 18.75 when neutrality or slant acidity; 55～60 ℃ of the most suitable temperature (general 10～85 ℃ all can), heat resistance is strong, also can complete deactivation in the time of 90 ℃; Oxidated dose of inhibition, reducing substances activates.
As preferably, in g/mL, the mass volume ratio of above-mentioned raw materials and water is 1: 2～8.
As preferably, the addition of AMS and pectase is 1.0～20.0 ten thousand IU/kg raw materials, and the addition of cellulase, carbohydrase, papain is 1.0～15.0 ten thousand IU/kg raw materials.
Preferably, the addition of AMS and pectase is 1.0～10.0 ten thousand IU/kg raw materials, and the addition of cellulase, carbohydrase, papain is 1.0～8.0 ten thousand IU/kg raw materials.
Preferably, the addition of AMS and pectase is 5.0～10.0 ten thousand IU/kg raw materials, and the addition of cellulase, carbohydrase, papain is 6.0～8.0 ten thousand IU/kg raw materials.
In the method provided by the invention, can pass through controlled enzymatic hydrolysis time controlled enzymatic hydrolysis process, if enzymolysis time is too short, easily cause the insufficient minimizing nutriment of enzymolysis yield; The long increase cost that can extend manufacture cycle again of enzymolysis time causes simultaneously the wasting of resources and increases contaminated probability.Therefore, the present invention incites somebody to action the enzymolysis time first time, enzymolysis time all is controlled to be 2～6h for the second time.Preferably, enzymolysis time is controlled to be 3.5～4.5h for the first time, and enzymolysis time is controlled to be 2.5～3.5h for the second time.
As preferably, the present invention extracts nutritional labeling by to carrying out enzymolysis after the seed of jackfruit and the luxuriant meat preliminary treatment, obtains the jackfruit extract through purification step.Purifying comprises that the DEAE chromatographic column separates, ultrafiltration step.By the DEAE chromatographic column separate, ultrafiltration step, polysaccharide, polypeptide, water soluble starch, cellulose, vitamin, mineral matter and micro-isoreactivity material that can the Fractional Collections small-molecular weight.
As preferably, described DEAE chromatographic column separates and adopts 10～30mmol/L, and the pH value is 6.8～7.2 phosphate buffer.
As preferably, it is the milipore filter of 50～100KD that molecular cut off is adopted in described ultrafiltration.
As preferably, also comprise filtration, centrifugation step before the described DEAE chromatographic column separation.
As preferably, described alkali is one or more the mixed base in NaOH, calcium hydroxide, potassium hydroxide, sodium acid carbonate, the ammoniacal liquor.
As preferably, described acid is one or more the mixed acid in hydrochloric acid, sulfuric acid, phosphoric acid, the citric acid.
Processing method of jack-fruit provided by the invention carries out enzymolysis and subsequent purification step by seed and the luxuriant meat to jackfruit, obtains the jackfruit extract.Evidence, processing method of jack-fruit provided by the invention, the recovery rate of jackfruit extract are 69.27～71.62%; In every 100mL jackfruit extract, contain polysaccharide 1015～1132mg, polypeptide 480～533mg, water soluble starch 73～82mg, cellulose 13～17mg, vitamin 8～13mg; In the jackfruit extract that obtains, contain Ca 0.0321～0.0334%, Mg 0.0178～0.0213%, and Na 0.0042～0.0045%, and Zn 0.0037～0.0044%.Jackfruit provided by the invention is processed the method, has improved the content of polysaccharide, polypeptide, water soluble starch, cellulose, vitamin, mineral matter in the jackfruit extract.
The specific embodiment
The invention discloses a kind of processing method of jack-fruit, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is, all similarly replace and change apparent to those skilled in the art, and they all are deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, the related personnel obviously can change or suitably change and combination methods and applications as herein described within not breaking away from content of the present invention, spirit and scope, realizes and use the technology of the present invention.
In the processing method of jack-fruit provided by the invention, pancreatin, AMS, pectase, cellulase, carbohydrase, papain are commercially available.Wherein, pancreatin is provided by Chongqing City strength health Bioisystech Co., Ltd, and batch number is 20100312; AMS is provided by Jining and U.S. bioengineering Co., Ltd, and batch number is 20100322; Pectase is provided by permanent magnificent road, Nanning east biotechnology Co., Ltd, and batch number is 20100405; Cellulase is provided by permanent magnificent road, Nanning east biotechnology Co., Ltd, and batch number is 20100416; Carbohydrase is provided by Jining and U.S. bioengineering Co., Ltd, and batch number is 20100325; Papain is provided by permanent magnificent road, Nanning east biotechnology Co., Ltd, and batch number is 20100325.
Below in conjunction with embodiment, further set forth the present invention:
Embodiment 1
Choose one of ripe jackfruit fruit, 8.6kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked 30min with 0.5% sodium acid carbonate after heating boil 5min, 85 ℃ of baking 1h rub with the hands after the cooling and remove the seed epidermis, and luxuriant meat and seed are mixed to get raw material, are weighed as 4.19kg; Add 14.66L distilled water, add distilled water after the making beating, obtain stoste 18.66L, be weighed as 18.85kg; Stoste is warming up to 47 ℃, with 0.1mol/L NaOH stoste is regulated pH value to 8.7, add the pancreatin of 40,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), add the pancreatin of 16.76 ten thousand IU in the stoste, after mixing, be 8.7 in the pH value, temperature is enzymolysis 3h under 47 ℃ the condition, obtains the first enzymolysis liquid; With 0.1mol/L HCl the pH value of the first enzymolysis liquid is adjusted to 4.1 again, add respectively pectase and the amylase of 100,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), in stoste, add respectively the pectase of 41.9 ten thousand IU and the amylase of 41.9 ten thousand IU, after mixing, be 4.1 in the pH value, temperature is enzymolysis 3h under 47 ℃ the condition, obtains the second enzymolysis liquid; Enzymolysis is heated to 100 ℃ with stoste after finishing, and insulation 10min carries out enzyme-deactivating, is cooled to below 30 ℃, regulates the pH value with 0.1mol/L NaOH and is 7.2, and is centrifugal, collects supernatant and gets 14.25kg; Carry out coarse filtration with plate filter, collect filter liquor and get 13.90kg; With water for injection with DEAE furnishing pasty state, adopt slurry type method dress post, dress column pressure 0.5Mpa, be the cleaning of 7.0 phosphate buffer with 400mmol/L pH value first behind the dress post, be 7.0 phosphate buffer balance again with 20mmol/L pH value, DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and is 7.0 phosphate buffer wash-out with 20mmol/L pH value.Collecting object and get 13.70kg, is that the milipore filter of 100KD carries out ultrafiltration with molecular cut off, collects ultrafiltrate 13.50kg, acquisition jackfruit extract.
Gained jackfruit extract is detected, the results are shown in Table 1.
Table 1 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1022 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (Forint phenol method), mg/100mL | 520 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 7.05 |
| Water soluble starch, mg/100mL | 80 |
| Cellulose, mg/100mL | 13 |
| Vitamin, mg/100mL | 10 |
| Ca，％ | 0.0321 |
| Mg，％ | 0.0186 |
| Na，％ | 0.0043 |
| Zn，％ | 0.0042 |
Embodiment 2
Choose one of ripe jackfruit fruit, 9.58kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked 30min with 0.5% sodium acid carbonate after heating boil 5min, 85 ℃ of baking 1h rub with the hands after the cooling and remove the seed epidermis, and luxuriant meat and seed are mixed to get raw material, are weighed as 4.55kg; Add 15.93L distilled water, add distilled water after the making beating, obtain stoste 19.90L, be weighed as 20.50kg; Stoste is warming up to 43 ℃, with 30%Ca (OH)
2Stoste is regulated pH value to 8.58, add the pancreatin of 40,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), add the pancreatin of 18.2 ten thousand IU in the stoste, after mixing, be 8.3 in the pH value, temperature is enzymolysis 3h under 43 ℃ the condition, obtains the first enzymolysis liquid; Use again 0.5mol/L H
3PO
4The pH value of the first enzymolysis liquid is adjusted to 4.0, add respectively the cellulase of 80,000 IU and the carbohydrase of 60,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), in stoste, add respectively the cellulase of 36.4 ten thousand IU and the carbohydrase of 27.3 ten thousand IU, after mixing, be 3.8 in the pH value, temperature is enzymolysis 3h under 43 ℃ the condition, obtains the second enzymolysis liquid; Enzymolysis is heated to 100 ℃ with stoste after finishing, and insulation 10min carries out enzyme-deactivating, is cooled to below 30 ℃, with 0.1mol/L Ca (OH)
2Regulate the pH value and be 6.8, centrifugal, collect supernatant and get 15.63kg; Carry out coarse filtration with plate filter, collect filter liquor and get 15.00kg; With water for injection with DEAE furnishing pasty state, adopt slurry type method dress post, dress column pressure 0.5Mpa, be the cleaning of 7.0 phosphate buffer with 400mmol/L pH value first behind the dress post, be 7.0 phosphate buffer balance again with 20mmol/L pH value, DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and is 7.0 phosphate buffer wash-out with 20mmol/L pH value.Collecting object and get 14.50kg, is that the milipore filter of 50KD carries out ultrafiltration with molecular cut off, collects ultrafiltrate 14.20kg, acquisition jackfruit extract.
Gained jackfruit extract is detected, the results are shown in Table 2.
Table 2 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1132 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (Forint phenol method), mg/100mL | 480 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 6.92 |
| Water soluble starch, mg/100mL | 73 |
| Cellulose, mg/100mL | 14 |
| Vitamin, mg/100mL | 8 |
| Ca，％ | 0.0332 |
| Mg，％ | 0.0213 |
| Na，％ | 0.0042 |
| Zn，％ | 0.0037 |
Embodiment 3
Choose one of ripe jackfruit fruit, 7.9kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked 30min with 0.5% sodium acid carbonate after heating boil 5min, 85 ℃ of baking 1h rub with the hands after the cooling and remove the seed epidermis, and luxuriant meat and seed are mixed to get raw material, are weighed as 3.85kg; Add 23.1L distilled water, add distilled water after the making beating, obtain stoste 26.01L, be weighed as 26.95kg; Stoste is warming up to 45 ℃, with 0.1mol/L KOH stoste is regulated pH value to 8.3, add the pancreatin of 100,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), add the pancreatin of 38.5 ten thousand IU in the stoste, after mixing, be 8.3 in the pH value, temperature is enzymolysis 5h under 43 ℃ the condition, obtains the first enzymolysis liquid; Use again 0.1mol/LH
2SO
4The pH value of the first enzymolysis liquid is adjusted to 4.1, add respectively pectase and the amylase of 150,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), in stoste, add respectively the pectase of 57.75 ten thousand IU and the amylase of 57.75 ten thousand IU, after mixing, be 3.8 in the pH value, temperature is enzymolysis 6h under 43 ℃ the condition, obtains the second enzymolysis liquid; Enzymolysis is heated to 100 ℃ with stoste after finishing, and insulation 10min carries out enzyme-deactivating, is cooled to below 30 ℃, uses 0.1mol/L NaHCO
3Regulate the pH value and be 7.2, centrifugal, collect supernatant and get 21.25kg; Carry out coarse filtration with plate filter, collect filter liquor and get 19.55kg; With water for injection with DEAE furnishing pasty state, adopt slurry type method dress post, dress column pressure 0.5Mpa, be the cleaning of 6.8 phosphate buffer with 400mmol/L pH value first behind the dress post, be 7.2 phosphate buffer balance again with 30mmol/L pH value, DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and is 7.2 phosphate buffer wash-out with 30mmol/L pH value.Collecting object and get 19.30kg, is that the milipore filter of 100KD carries out ultrafiltration with molecular cut off, collects ultrafiltrate 19.05kg, acquisition jackfruit extract.
Gained jackfruit extract is detected, the results are shown in Table 3.
Table 3 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1085 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (Forint phenol method), mg/100mL | 496 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 7.02 |
| Water soluble starch, mg/100mL | 76 |
| Cellulose, mg/100mL | 15 |
| Vitamin, mg/100mL | 12 |
| Ca，％ | 0.0327 |
| Mg，％ | 0.0192 |
| Na，％ | 0.0045 |
| Zn，％ | 0.0044 |
Embodiment 4
Choose one of ripe jackfruit fruit, 10.46kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked 30min with 0.5% sodium acid carbonate after heating boil 5min, 85 ℃ of baking 1h rub with the hands after the cooling and remove the seed epidermis, and luxuriant meat and seed are mixed to get raw material, are weighed as 5.71kg; Add 45.68L distilled water, add distilled water after the making beating, obtain stoste 50.88L, be weighed as 51.39kg; Stoste is warming up to 44 ℃, with 0.1mol/L ammoniacal liquor stoste is regulated pH value to 8.4, add the pancreatin of 100,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), add the pancreatin of 57.1 ten thousand IU in the stoste, after mixing, be 8.4 in the pH value, temperature is enzymolysis 6h under 44 ℃ the condition, obtains the first enzymolysis liquid; With the 0.1mol/L citric acid pH value of the first enzymolysis liquid is adjusted to 4.1 again, add respectively pectase and the amylase of 200,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), in stoste, add respectively the pectase of 114.2 ten thousand IU and the amylase of 114.2 ten thousand IU, after mixing, be 3.9 in the pH value, temperature is enzymolysis 6h under 44 ℃ the condition, obtains the second enzymolysis liquid; Enzymolysis is heated to 100 ℃ with stoste after finishing, and insulation 10min carries out enzyme-deactivating, is cooled to below 30 ℃, uses 0.1mol/L NaHCO
3Regulate the pH value and be 6.8, centrifugal, collect supernatant and get 37.25kg; Carry out coarse filtration with plate filter, collect filter liquor and get 36.76kg; With water for injection with DEAE furnishing pasty state, adopt slurry type method dress post, dress column pressure 0.5Mpa, be the cleaning of 7.2 phosphate buffer with 400mmol/L pH value first behind the dress post, be 6.8 phosphate buffer balance again with 10mmol/L pH value, DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and is 6.8 phosphate buffer wash-out with 10mmol/L pH value.Collecting object and get 36.40kg, is that the milipore filter of 80KD carries out ultrafiltration with molecular cut off, collects ultrafiltrate 36.25kg, acquisition jackfruit extract.
Gained jackfruit extract is detected, the results are shown in Table 4.
Table 4 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1053 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (Forint phenol method), mg/100mL | 533 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 6.95 |
| Water soluble starch, mg/100mL | 82 |
| Cellulose, mg/100mL | 17 |
| Vitamin, mg/100mL | 13 |
| Ca，％ | 0.0330 |
| Mg，％ | 0.0196 |
| Na，％ | 0.0045 |
| Zn，％ | 0.0043 |
Embodiment 5
Choose one of ripe jackfruit fruit, 8.48kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked 30min with 0.5% sodium acid carbonate after heating boil 5min, 85 ℃ of baking 1h rub with the hands after the cooling and remove the seed epidermis, and luxuriant meat and seed are mixed to get raw material, are weighed as 3.8kg; Add 11.4L distilled water, add distilled water after the making beating, obtain stoste 14.92L, be weighed as 15.2kg; Stoste is warming up to 46 ℃, with 0.1mol/L NaOH stoste is regulated pH value to 8.6, add the pancreatin of 20,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), add the pancreatin of 7.6 ten thousand IU in the stoste, after mixing, be 8.6 in the pH value, temperature is enzymolysis 2h under 46 ℃ the condition, obtains the first enzymolysis liquid; With 0.1mol/L HCl the pH value of the first enzymolysis liquid is adjusted to 3.9 again, add respectively cellulase and the carbohydrase of 20,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), in stoste, add respectively the cellulase of 7.6 ten thousand IU and the carbohydrase of 7.6 ten thousand IU, after mixing, be 4.1 in the pH value, temperature is enzymolysis 3h under 47 ℃ the condition, obtains the second enzymolysis liquid; Enzymolysis is heated to 100 ℃ with stoste after finishing, and insulation 10min carries out enzyme-deactivating, is cooled to below 30 ℃, regulates the pH value with 0.1mol/L NaOH and is 6.8, and is centrifugal, collects supernatant and gets 11.3kg; Carry out coarse filtration with plate filter, collect filter liquor and get 11.1kg; With water for injection with DEAE furnishing pasty state, adopt slurry type method dress post, dress column pressure 0.5Mpa, be the cleaning of 6.8 phosphate buffer with 400mmol/L pH value first behind the dress post, be 6.9 phosphate buffer balance again with 15mmol/L pH value, DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and is 6.9 phosphate buffer wash-out with 15mmol/L pH value.Collecting object and get 10.96kg, is that the milipore filter of 90KD carries out ultrafiltration with molecular cut off, collects ultrafiltrate 10.85kg, acquisition jackfruit extract.
Gained jackfruit extract is detected, the results are shown in Table 5.
Table 5 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1019 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (Forint phenol method), mg/100mL | 527 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 7.00 |
| Water soluble starch, mg/100mL | 78 |
| Cellulose, mg/100mL | 16 |
| Vitamin, mg/100mL | 12 |
| Ca，％ | 0.0325 |
| Mg，％ | 0.0192 |
| Na，％ | 0.0044 |
| Zn，％ | 0.0041 |
Embodiment 6
Choose one of ripe jackfruit fruit, 9.5kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked 30min with 0.5% sodium acid carbonate after heating boil 5min, 85 ℃ of baking 1h rub with the hands after the cooling and remove the seed epidermis, and luxuriant meat and seed are mixed to get raw material, are weighed as 4.7kg; Add 9.42L distilled water, add distilled water after the making beating, obtain stoste 13.01L, be weighed as 13.42kg; Stoste is warming up to 47 ℃, with 0.1mol/L NaOH stoste is regulated pH value to 8.7, add the pancreatin of 60,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), add the pancreatin of 28.2 ten thousand IU in the stoste, after mixing, be 8.7 in the pH value, temperature is enzymolysis 4h under 47 ℃ the condition, obtains the first enzymolysis liquid; With 0.1mol/LHCl the pH value of the first enzymolysis liquid is adjusted to 4.2 again, add respectively cellulase and the papain of 150,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), in stoste, add respectively the cellulase of 70.6 ten thousand IU and the papain of 70.6 ten thousand IU, after mixing, be 4.2 in the pH value, temperature is enzymolysis 3h under 47 ℃ the condition, obtains the second enzymolysis liquid; Enzymolysis is heated to 100 ℃ with stoste after finishing, and insulation 10min carries out enzyme-deactivating, is cooled to below 30 ℃, regulates the pH value with 0.1mol/L NaOH and is 7.2, and is centrifugal, collects supernatant and gets 9.89kg; Carry out coarse filtration with plate filter, collect filter liquor and get 9.76kg; With water for injection with DEAE furnishing pasty state, adopt slurry type method dress post, dress column pressure 0.5Mpa, be the cleaning of 7.0 phosphate buffer with 400mmol/L pH value first behind the dress post, be 7.0 phosphate buffer balance again with 20mmol/L pH value, DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and is 7.0 phosphate buffer wash-out with 20mmol/L pH value.Collecting object and get 9.66kg, is that the milipore filter of 100KD carries out ultrafiltration with molecular cut off, collects ultrafiltrate 9.48kg, acquisition jackfruit extract.
Gained jackfruit extract is detected, the results are shown in Table 6.
Table 6 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1015 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (Forint phenol method), mg/100mL | 515 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 6.95 |
| Water soluble starch, mg/100mL | 82 |
| Cellulose, mg/100mL | 12 |
| Vitamin, mg/100mL | 12 |
| Ca，％ | 0.0334 |
| Mg，％ | 0.0178 |
| Na，％ | 0.0042 |
| Zn，％ | 0.0043 |
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a processing method of jack-fruit is characterized in that, comprising:
Step 1: the seed of jackfruit is soaked 30min with 0.5～1% sodium bicarbonate solution, remove epidermis behind the heating and cooling, be mixed to get raw material with the luxuriant meat of jackfruit after cleaning oven dry, in raw material, add entry and make stoste;
Step 2: adding alkali in described stoste, to make the pH value of described stoste be 8.3～8.7, under 43～47 ℃, adds the pancreatin of 1.0～10.0 ten thousand IU in every kg raw material, and enzymolysis 2～6h obtains the first enzymolysis liquid;
Step 3: adding acid in described the first enzymolysis liquid, to make the pH value of described the first enzymolysis liquid be 3.8～4.2, under 43～47 ℃, add in the AMS, pectase, cellulase, carbohydrase, papain of 1.0～20.0 ten thousand IU one or more in every kg raw material, enzymolysis 2～6h obtains the second enzymolysis liquid;
Step 4: described the second enzymolysis liquid is made the jackfruit extract after the separation of DEAE chromatographic column, ultrafiltration.
2. the method for claim 1 is characterized in that, in g/mL, the mass volume ratio of raw material and water is 1:2～8.
3. the method for claim 1 is characterized in that, described DEAE chromatographic column separates employing 10～30mmol/L, and the pH value is 6.8～7.2 phosphate buffer.
4. the method for claim 1 is characterized in that, it is the milipore filter of 50～100kD that molecular cut off is adopted in described ultrafiltration.
5. the method for claim 1 is characterized in that, described DEAE chromatographic column also comprises filtration, centrifugation step before separating.
6. the method for claim 1 is characterized in that, described alkali is one or more the mixed base in NaOH, calcium hydroxide, potassium hydroxide, sodium acid carbonate, the ammoniacal liquor.
7. the method for claim 1 is characterized in that, described acid is one or more the mixed acid in hydrochloric acid, sulfuric acid, phosphoric acid, the citric acid.
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| CN103431328B (en) * | 2013-09-02 | 2014-11-05 | 中国热带农业科学院农产品加工研究所 | Natural jackfruit powder and preparation method thereof |
| CN103710214B (en) * | 2014-01-06 | 2016-05-11 | 中国热带农业科学院香料饮料研究所 | A kind of jackfruit fruit wine and preparation method thereof |
| CN103815007B (en) * | 2014-03-13 | 2015-07-15 | 中国热带农业科学院香料饮料研究所 | Canned black tea and jackfruit seeds in syrup and preparation method thereof |
| CN105063129A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Ultrasonic extraction process of kudzuvine root fructose |
| CN110357938B (en) * | 2019-08-30 | 2021-05-04 | 中国热带农业科学院香料饮料研究所 | Method for extracting protein from jack fruit seeds |
| CN110656138A (en) * | 2019-09-26 | 2020-01-07 | 海南农垦南金农场有限公司 | Method for preparing high-purity resistant starch by using jackfruit seed starch |
| CN112971133A (en) * | 2021-04-19 | 2021-06-18 | 海南云皓生物科技有限公司 | Paederia scandens enzyme, preparation method thereof and application of paederia scandens enzyme in preparation of anti-inflammatory and analgesic products |
| CN114098075A (en) * | 2021-11-09 | 2022-03-01 | 安徽盛美诺生物技术有限公司 | Jackfruit-based compound preparation with immunocompetence and application thereof |
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