CN102318846A - Processing method of jack-fruit - Google Patents
Processing method of jack-fruit Download PDFInfo
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- CN102318846A CN102318846A CN201110150821A CN201110150821A CN102318846A CN 102318846 A CN102318846 A CN 102318846A CN 201110150821 A CN201110150821 A CN 201110150821A CN 201110150821 A CN201110150821 A CN 201110150821A CN 102318846 A CN102318846 A CN 102318846A
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- jackfruit
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Abstract
The invention relates to the processing field of food, and in particular to a processing method of jack-fruit. Enzymatic hydrolysis and subsequent purification procedures are undertaken on seeds and bud meat of jack-fruit, so contents of amylose, polypeptide, aqueous soluble starch, cellulose, vitamins and minerals in the jack-fruit extracts can be improved.
Description
Technical field
The present invention relates to food processing field, particularly a kind of jackfruit processing method.
Background technology
Jackfruit is typical tropical fruit (tree), originates in India, and tropic countries such as Sri Lanka, Burma, Indonesia all have cultivation at present.China's cultivation jackfruit is the history in existing more than 1,000 year so far, and all there is cultivation in the torrid zone, the south subtropics area of existing Guangdong, Guangxi, Hainan, Yunnan, Fujian and South Sichuan, and Hainan Province's plantation at most.
Modern medicine study confirms, contains rich saccharide, protein, B family vitamin (B in the jackfruit
1, B
2, B
6), vitamin C, mineral matter, fatty wet goods, the normal physiological function of keeping body is had important function.The hydrolysis of ability intensive aspect inner fibrin after the edible jackfruit; Can be with blocking in tissue and endovascular fibrin and dissolution of blood clot; Thereby improve local blood, body fluid circulation; Make inflammation and oedema absorb, disappear, cerebral thrombus and the caused disease of other thrombus are also had certain supplemental treatment effect.
Pulp contains total reducing sugar 20.5～21.7%, protein 1.05～1.72%, fat 0.6%, carbohydrate 23.4%, ash content 0.5%, cellulose 1.8% in the jackfruit fruit; Seed carbohydrate containing 38.4%, protein 6.6%, fat 0.4%, cellulose 1.5%.But; At present to the exploitation of jackfruit bud meat only be through to the drying of jackfruit bud meat, toast and process luxuriant sliced meat or sugar is processed preserved fruit; Exploitation to the jackfruit seed just makes starch through the method for physics; Process is destroyed the nutritional labeling in jackfruit bud meat and the seed easily, further influences the recovery rate of each nutritional labeling.
Summary of the invention
In view of this, the present invention provides a kind of jackfruit processing method.This method is carried out enzymolysis and subsequent purification step through seed and the luxuriant meat to jackfruit, has improved the content of nutritional labelings such as polysaccharide, polypeptide in the jackfruit extract.
In order to realize the foregoing invention purpose, the present invention provides following technical scheme:
The invention provides a kind of jackfruit processing method, comprising:
Step 1: the seed of jackfruit is soaked 30min with 0.5～1% sodium bicarbonate solution, remove epidermis after the heating cooling, clean the oven dry back and mix with the luxuriant meat of jackfruit and obtain raw material, in raw material, add entry and make stoste;
Step 2: in said stoste, adding alkali, to make the pH value of said stoste be 8.3～8.7, under 43～47 ℃, adds 1.0～10.0 ten thousand IU pancreatin in every kg raw material, and enzymolysis 2～6h obtains first enzymolysis liquid;
Step 3: in said first enzymolysis liquid, adding acid, to make the pH value of said first enzymolysis liquid be 3.8～4.2; Under 43～47 ℃; Add in the AMS, pectase, cellulase, carbohydrase, papain of 1.0～20.0 ten thousand IU one or more in every kg raw material; Enzymolysis 2～6h obtains second enzymolysis liquid;
Step 4: said second enzymolysis liquid is made the jackfruit extract through the separation of DEAE chromatographic column, ultrafiltration.
Pancreatin, be through extract, refining, the composite a kind of complex enzyme that forms, contain protease, lipase, amylase etc., have abilities such as stronger decomposing protein and fat, be widely used in food, medical treatment, brewage, industries such as silk, process hides.Jackfruit processing method provided by the invention, in the pancreatin of selection, in enzyme activity (IU), trypsase: amylopsin: pancreatic lipase=6: 70: 40.Preferably, in the method provided by the invention, in every 1g pancreatin, tryptic enzyme activity is 600～800IU, and the enzyme activity of amylopsin is 7000～9000IU, and the enzyme activity of pancreatic lipase is 4000～6000IU.
AMS, numbering: EC 3.2.1.1, diastatic a kind of, be endo-amylase, catalysis random hydrolysis α-1, the 4-glycosidic bond produces maltose, maltotriose and schardinger dextrin.Wherein, fungal alpha-amylase is a kind of AMS that is produced by the aspergillus microbial fermentation.Different with bacterial is that the optimum temperature of fungal alpha-amylase is about 40～55 ℃, surpasses 60 ℃ of beginning inactivations; The maltose that its amylatic product mainly is a high-load and some compound sugar and a spot of glucose.And bacterial optimum temperature high (70～80 ℃ of middle temperature AMSs, high temperature resistant AMS are 95～105 ℃), amylatic primary product is a dextrin.Therefore, bacterial can only be used for fermentation industry, and fungal alpha-amylase then is widely used in the production of starch syrup, compound sugar, beer, bakery, Flour product etc.In the jackfruit processing method provided by the invention, select for use fungal alpha-amylase to carry out enzymolysis.
Pectase, a multienzyme complex of decompose pectin generally includes protopectinase, pectinesterase hydrolase, pectinesterase, makes pectic substance be able to decompose fully through their synergy.Natural pectic substance changes into the pectin of water dissolvable under the protopectinase effect; Pectin is generated pectic acid by pectin methyl esters hydrolase catalysis removing methyl esters group; Pectic acid generates galacturonic acid through pectic acid hydrolase and the degraded of pectate lyase class.Pectase can be made with extra care and get through fermentation by aspergillus niger (Aspergillus niger), and decompose pectin is had good effect.Action pH value is: 2.5～6.0, and the righttest action pH value is: 3.5～4.5.
Cellulase is a kind of complex enzyme, mainly is made up of circumscribed 1,4 beta-glucanase, inscribe 1,4 beta-glucanase and beta-glucosidase etc., also has the Xylanase activity of very high vigor.Endoglucanase is the inner unformed area of the plain polysaccharide chain of cutting fibre at random, produces the oligosaccharides of different length and the end of new chain.Exoglucanase acts on the end of the fibrination sugar chain of these reproducibilities and irreducibility, discharges glucose or cellobiose.Beta-glucosidase can be decomposed into glucose with cellobiose, cellotriose and other low molecule cellodextrins.
Carbohydrase is claimed glucoamylase again, and formal name used at school is α-1,4-glucose hydrolysis enzyme (α-1; 4-Glucan glucohydrolace), can produce glucose to starch from non reducing end hydrolyzing alpha-1,4 glucoside bond; Also slow hydrolyzing alpha-1,6 glucoside bond is converted into glucose.The Substratspezifitaet of carbohydrase is lower, it except can from the irreducibility of starch chain not end-grain cutting open α-1,4 key place, also can slowly cut α-1,6.Therefore, grape unit is downcut in not holding amylose from irreducibility that it can be very fast successively, cuts apart running into 1,6 key, earlier α-1,6 key is cut apart, and α-1,4 key is cut apart again, thereby is made amylopectin be hydrolyzed into glucose.Carbohydrase increases with temperature rising vigor, surpasses 65 ℃ and sharply descends with temperature rising vigor again, and optimum temperature is 60～62 ℃, optimum pH 4.0～4.5.
Papain is called for short papain, claims Fructus Chaenomelis ferment again; Being a kind ofly can antibody molecule be hydrolyzed to 3 fragments at acid, neutral, alkaline environment equal proteolytic enzyme of ability decomposing protein down, is a kind of low specific proteins hydrolase that contains in the papaya; The activated centre contains cysteine; Belong to thiol protease, have the substrate specificity of broad, act on the peptide bond that L-arginine in the protein, L-lysine, glycine and L-citrulling residue are participated in formation; Papain belongs to endopeptidase, can cut whole-egg protein matter intramolecule peptide chain-CO-NH-and generate molecular weight smaller polypeptides class.The mechanism of the shearing peptide bond of papain comprises: Cys-25 deprotonation under the His-159 effect, and Asn-158 can help the putting of imidazole ring of His-159, and makes deprotonation to take place; Carbonyl carbon on the Cys-25 nucleophillic attack peptide main chain, and covalently bound with it formation acyl group-enzyme intermediate then; Removal of acylation takes place in then enzyme and hydrone effect, and discharges the carbonyl end of peptide chain.Water-soluble and the glycerine of papain, the aqueous solution is colourless or faint yellow, is creamy white sometimes; Be dissolved in organic solvents such as ethanol, chloroform and ether hardly; The most suitable pH value is 5.7 (general 3～9.5 all can), effect is also arranged, isoelectric point 18.75 when neutrality or slant acidity; 55～60 ℃ of the most suitable temperature (general 10～85 ℃ all can), hear resistance is strong, also can complete deactivation in the time of 90 ℃; Oxidated dose of inhibition, reducing substances activates.
As preferably, in g/mL, the mass volume ratio of above-mentioned raw materials and water is 1: 2～8.
As preferably, the addition of AMS and pectase is 1.0～20.0 ten thousand IU/kg raw materials, and the addition of cellulase, carbohydrase, papain is 1.0～15.0 ten thousand IU/kg raw materials.
Preferably, the addition of AMS and pectase is 1.0～10.0 ten thousand IU/kg raw materials, and the addition of cellulase, carbohydrase, papain is 1.0～8.0 ten thousand IU/kg raw materials.
Preferably, the addition of AMS and pectase is 5.0～10.0 ten thousand IU/kg raw materials, and the addition of cellulase, carbohydrase, papain is 6.0～8.0 ten thousand IU/kg raw materials.
In the method provided by the invention, can if enzymolysis time is too short, cause the insufficient minimizing nutriment of enzymolysis yield easily through control enzymolysis time control enzymolysis process; The long increase cost that can extend manufacture cycle again of enzymolysis time causes the wasting of resources simultaneously and increases contaminated probability.Therefore, the present invention incites somebody to action the enzymolysis time first time, enzymolysis time all is controlled to be 2～6h for the second time.Preferably, enzymolysis time is controlled to be 3.5～4.5h for the first time, and enzymolysis time is controlled to be 2.5～3.5h for the second time.
As preferably, the present invention extracts nutritional labeling through to carrying out enzymolysis after the seed of jackfruit and the luxuriant meat preliminary treatment, obtains the jackfruit extract through purification step.Purifying comprises that the DEAE chromatographic column separates, ultrafiltration step.Through the DEAE chromatographic column separate, ultrafiltration step, polysaccharide, polypeptide, water soluble starch, cellulose, vitamin, mineral matter and micro-isoreactivity material that can the Fractional Collections small-molecular weight.
As preferably, said DEAE chromatographic column separates and adopts 10～30mmol/L, and the pH value is 6.8～7.2 phosphate buffer.
As preferably, it is the milipore filter of 50～100KD that molecular cut off is adopted in said ultrafiltration.
As preferably, also comprise filtration, centrifugation step before the said DEAE chromatographic column separation.
As preferably, said alkali is one or more the mixed base in NaOH, calcium hydroxide, potassium hydroxide, sodium acid carbonate, the ammoniacal liquor.
As preferably, said acid is one or more the mixed acid in hydrochloric acid, sulfuric acid, phosphoric acid, the citric acid.
Jackfruit processing method provided by the invention is carried out enzymolysis and subsequent purification step through seed and the luxuriant meat to jackfruit, obtains the jackfruit extract.Evidence, jackfruit processing method provided by the invention, the recovery rate of jackfruit extract is 69.27～71.62%; In every 100mL jackfruit extract, contain polysaccharide 1015～1132mg, polypeptide 480～533mg, water soluble starch 73～82mg, cellulose 13～17mg, vitamin 8～13mg; In the jackfruit extract that obtains, contain Ca 0.0321～0.0334%, Mg 0.0178～0.0213%, and Na 0.0042～0.0045%, and Zn 0.0037～0.0044%.Jackfruit provided by the invention is processed this method, has improved the content of polysaccharide, polypeptide, water soluble starch, cellulose, vitamin, mineral matter in the jackfruit extract.
The specific embodiment
The invention discloses a kind of jackfruit processing method, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as and are included in the present invention.Method of the present invention and application are described through preferred embodiment; The related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use technology of the present invention.
In the jackfruit processing method provided by the invention, pancreatin, AMS, pectase, cellulase, carbohydrase, papain are commercially available.Wherein, pancreatin is provided by Chongqing City strength health Bioisystech Co., Ltd, and batch number is 20100312; AMS is provided by Jining and U.S. bioengineering Co., Ltd, and batch number is 20100322; Pectase is provided by permanent magnificent road, Nanning east biotechnology Co., Ltd, and batch number is 20100405; Cellulase is provided by permanent magnificent road, Nanning east biotechnology Co., Ltd, and batch number is 20100416; Carbohydrase is provided by Jining and U.S. bioengineering Co., Ltd, and batch number is 20100325; Papain is provided by permanent magnificent road, Nanning east biotechnology Co., Ltd, and batch number is 20100325.
Below in conjunction with embodiment, further set forth the present invention:
Embodiment 1
Choose one of ripe jackfruit fruit, 8.6kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked heated and boiled 5min behind the 30min with 0.5% sodium acid carbonate, 85 ℃ of baking 1h, the cooling back is rubbed with the hands and is removed the seed epidermis, and mixing obtains raw material with seed with luxuriant meat, is weighed as 4.19kg; Add 14.66L distilled water, distilled water is added in the making beating back, obtains stoste 18.66L, is weighed as 18.85kg; Stoste is warming up to 47 ℃; With 0.1mol/L NaOH stoste is regulated pH value to 8.7,, in stoste, add the pancreatin of 16.76 ten thousand IU according to the pancreatin of every kg raw material (gross weight of luxuriant meat and seed) interpolation 40,000 IU; After mixing; In the pH value is 8.7, and temperature is enzymolysis 3h under 47 ℃ the condition, obtains first enzymolysis liquid; With 0.1mol/L HCl the pH value of first enzymolysis liquid is adjusted to 4.1 again; Add pectase and the amylase of 100,000 IU respectively according to every kg raw material (gross weight of luxuriant meat and seed); In stoste, adding the pectase of 41.9 ten thousand IU and the amylase of 41.9 ten thousand IU respectively, after mixing, is 4.1 in the pH value; Temperature is enzymolysis 3h under 47 ℃ the condition, obtains second enzymolysis liquid; After enzymolysis finished, with stoste heat temperature raising to 100 ℃, insulation 10min carried out enzyme-deactivating, is cooled to below 30 ℃, uses 0.1mol/L NaOH to regulate pH value and is 7.2, and centrifugal, the collection supernatant gets 14.25kg; Carry out coarse filtration with plate filter, collect filter liquor and get 13.90kg; With water for injection with DEAE furnishing pasty state; Adopt slurry type method dress post; Dress column pressure 0.5Mpa, using 400mmol/L pH value behind the dress post earlier is 7.0 phosphate buffer cleaning, using 20mmol/L pH value again is 7.0 phosphate buffer balance; DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and use 20mmol/L pH value is 7.0 phosphate buffer wash-out.Collect object and get 13.70kg, use molecular cut off to carry out ultrafiltration, collect ultrafiltrate 13.50kg, obtain the jackfruit extract as the milipore filter of 100KD.
Gained jackfruit extract is detected, and the result sees table 1.
Table 1 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1022 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (forint phenol method), mg/100mL | 520 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 7.05 |
| Water soluble starch, mg/100mL | 80 |
| Cellulose, mg/100mL | 13 |
| Vitamin, mg/100mL | 10 |
| Ca，％ | 0.0321 |
| Mg，％ | 0.0186 |
| Na，％ | 0.0043 |
| Zn，％ | 0.0042 |
Embodiment 2
Choose one of ripe jackfruit fruit, 9.58kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked heated and boiled 5min behind the 30min with 0.5% sodium acid carbonate, 85 ℃ of baking 1h, the cooling back is rubbed with the hands and is removed the seed epidermis, and mixing obtains raw material with seed with luxuriant meat, is weighed as 4.55kg; Add 15.93L distilled water, distilled water is added in the making beating back, obtains stoste 19.90L, is weighed as 20.50kg; Stoste is warming up to 43 ℃, with 30%Ca (OH)
2Stoste being regulated pH value to 8.58, add the pancreatin of 40,000 IU according to every kg raw material (gross weight of luxuriant meat and seed), in stoste, add the pancreatin of 18.2 ten thousand IU, after mixing, is 8.3 in the pH value, and temperature is enzymolysis 3h under 43 ℃ the condition, obtains first enzymolysis liquid; Use 0.5mol/L H again
3PO
4The pH value of first enzymolysis liquid is adjusted to 4.0; Add the cellulase of 80,000 IU and the carbohydrase of 60,000 IU respectively according to every kg raw material (gross weight of luxuriant meat and seed); In stoste, adding the cellulase of 36.4 ten thousand IU and the carbohydrase of 27.3 ten thousand IU respectively, after mixing, is 3.8 in the pH value; Temperature is enzymolysis 3h under 43 ℃ the condition, obtains second enzymolysis liquid; After enzymolysis finished, with stoste heat temperature raising to 100 ℃, insulation 10min carried out enzyme-deactivating, is cooled to below 30 ℃, with 0.1mol/L Ca (OH)
2Regulate the pH value and be 6.8, centrifugal, collect supernatant and get 15.63kg; Carry out coarse filtration with plate filter, collect filter liquor and get 15.00kg; With water for injection with DEAE furnishing pasty state; Adopt slurry type method dress post; Dress column pressure 0.5Mpa, using 400mmol/L pH value behind the dress post earlier is 7.0 phosphate buffer cleaning, using 20mmol/L pH value again is 7.0 phosphate buffer balance; DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and use 20mmol/L pH value is 7.0 phosphate buffer wash-out.Collect object and get 14.50kg, use molecular cut off to carry out ultrafiltration, collect ultrafiltrate 14.20kg, obtain the jackfruit extract as the milipore filter of 50KD.
Gained jackfruit extract is detected, and the result sees table 2.
Table 2 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1132 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (forint phenol method), mg/100mL | 480 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 6.92 |
| Water soluble starch, mg/100mL | 73 |
| Cellulose, mg/100mL | 14 |
| Vitamin, mg/100mL | 8 |
| Ca，％ | 0.0332 |
| Mg，％ | 0.0213 |
| Na，％ | 0.0042 |
| Zn，％ | 0.0037 |
Embodiment 3
Choose one of ripe jackfruit fruit, 7.9kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked heated and boiled 5min behind the 30min with 0.5% sodium acid carbonate, 85 ℃ of baking 1h, the cooling back is rubbed with the hands and is removed the seed epidermis, and mixing obtains raw material with seed with luxuriant meat, is weighed as 3.85kg; Add 23.1L distilled water, distilled water is added in the making beating back, obtains stoste 26.01L, is weighed as 26.95kg; Stoste is warming up to 45 ℃; With 0.1mol/L KOH stoste is regulated pH value to 8.3,, in stoste, add the pancreatin of 38.5 ten thousand IU according to the pancreatin of every kg raw material (gross weight of luxuriant meat and seed) interpolation 100,000 IU; After mixing; In the pH value is 8.3, and temperature is enzymolysis 5h under 43 ℃ the condition, obtains first enzymolysis liquid; Use 0.1mol/LH again
2SO
4The pH value of first enzymolysis liquid is adjusted to 4.1; Add pectase and the amylase of 150,000 IU respectively according to every kg raw material (gross weight of luxuriant meat and seed); In stoste, adding the pectase of 57.75 ten thousand IU and the amylase of 57.75 ten thousand IU respectively, after mixing, is 3.8 in the pH value; Temperature is enzymolysis 6h under 43 ℃ the condition, obtains second enzymolysis liquid; After enzymolysis finished, with stoste heat temperature raising to 100 ℃, insulation 10min carried out enzyme-deactivating, is cooled to below 30 ℃, uses 0.1mol/L NaHCO
3Regulate the pH value and be 7.2, centrifugal, collect supernatant and get 21.25kg; Carry out coarse filtration with plate filter, collect filter liquor and get 19.55kg; With water for injection with DEAE furnishing pasty state; Adopt slurry type method dress post; Dress column pressure 0.5Mpa, using 400mmol/L pH value behind the dress post earlier is 6.8 phosphate buffer cleaning, using 30mmol/L pH value again is 7.2 phosphate buffer balance; DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and use 30mmol/L pH value is 7.2 phosphate buffer wash-out.Collect object and get 19.30kg, use molecular cut off to carry out ultrafiltration, collect ultrafiltrate 19.05kg, obtain the jackfruit extract as the milipore filter of 100KD.
Gained jackfruit extract is detected, and the result sees table 3.
Table 3 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1085 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (forint phenol method), mg/100mL | 496 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 7.02 |
| Water soluble starch, mg/100mL | 76 |
| Cellulose, mg/100mL | 15 |
| Vitamin, mg/100mL | 12 |
| Ca，％ | 0.0327 |
| Mg，％ | 0.0192 |
| Na，％ | 0.0045 |
| Zn，％ | 0.0044 |
Embodiment 4
Choose one of ripe jackfruit fruit, 10.46kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked heated and boiled 5min behind the 30min with 0.5% sodium acid carbonate, 85 ℃ of baking 1h, the cooling back is rubbed with the hands and is removed the seed epidermis, and mixing obtains raw material with seed with luxuriant meat, is weighed as 5.71kg; Add 45.68L distilled water, distilled water is added in the making beating back, obtains stoste 50.88L, is weighed as 51.39kg; Stoste is warming up to 44 ℃; With 0.1mol/L ammoniacal liquor stoste is regulated pH value to 8.4,, in stoste, add the pancreatin of 57.1 ten thousand IU according to the pancreatin of every kg raw material (gross weight of luxuriant meat and seed) interpolation 100,000 IU; After mixing; In the pH value is 8.4, and temperature is enzymolysis 6h under 44 ℃ the condition, obtains first enzymolysis liquid; With the 0.1mol/L citric acid pH value of first enzymolysis liquid is adjusted to 4.1 again; Add pectase and the amylase of 200,000 IU respectively according to every kg raw material (gross weight of luxuriant meat and seed); In stoste, adding the pectase of 114.2 ten thousand IU and the amylase of 114.2 ten thousand IU respectively, after mixing, is 3.9 in the pH value; Temperature is enzymolysis 6h under 44 ℃ the condition, obtains second enzymolysis liquid; After enzymolysis finished, with stoste heat temperature raising to 100 ℃, insulation 10min carried out enzyme-deactivating, is cooled to below 30 ℃, uses 0.1mol/L NaHCO
3Regulate the pH value and be 6.8, centrifugal, collect supernatant and get 37.25kg; Carry out coarse filtration with plate filter, collect filter liquor and get 36.76kg; With water for injection with DEAE furnishing pasty state; Adopt slurry type method dress post; Dress column pressure 0.5Mpa, using 400mmol/L pH value behind the dress post earlier is 7.2 phosphate buffer cleaning, using 10mmol/L pH value again is 6.8 phosphate buffer balance; DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and use 10mmol/L pH value is 6.8 phosphate buffer wash-out.Collect object and get 36.40kg, use molecular cut off to carry out ultrafiltration, collect ultrafiltrate 36.25kg, obtain the jackfruit extract as the milipore filter of 80KD.
Gained jackfruit extract is detected, and the result sees table 4.
Table 4 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1053 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (forint phenol method), mg/100mL | 533 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 6.95 |
| Water soluble starch, mg/100mL | 82 |
| Cellulose, mg/100mL | 17 |
| Vitamin, mg/100mL | 13 |
| Ca，％ | 0.0330 |
| Mg，％ | 0.0196 |
| Na，％ | 0.0045 |
| Zn，％ | 0.0043 |
Embodiment 5
Choose one of ripe jackfruit fruit, 8.48kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked heated and boiled 5min behind the 30min with 0.5% sodium acid carbonate, 85 ℃ of baking 1h, the cooling back is rubbed with the hands and is removed the seed epidermis, and mixing obtains raw material with seed with luxuriant meat, is weighed as 3.8kg; Add 11.4L distilled water, distilled water is added in the making beating back, obtains stoste 14.92L, is weighed as 15.2kg; Stoste is warming up to 46 ℃; With 0.1mol/L NaOH stoste is regulated pH value to 8.6,, in stoste, add the pancreatin of 7.6 ten thousand IU according to the pancreatin of every kg raw material (gross weight of luxuriant meat and seed) interpolation 20,000 IU; After mixing; In the pH value is 8.6, and temperature is enzymolysis 2h under 46 ℃ the condition, obtains first enzymolysis liquid; With 0.1mol/L HCl the pH value of first enzymolysis liquid is adjusted to 3.9 again; Add cellulase and the carbohydrase of 20,000 IU respectively according to every kg raw material (gross weight of luxuriant meat and seed); In stoste, adding the cellulase of 7.6 ten thousand IU and the carbohydrase of 7.6 ten thousand IU respectively, after mixing, is 4.1 in the pH value; Temperature is enzymolysis 3h under 47 ℃ the condition, obtains second enzymolysis liquid; After enzymolysis finished, with stoste heat temperature raising to 100 ℃, insulation 10min carried out enzyme-deactivating, is cooled to below 30 ℃, uses 0.1mol/L NaOH to regulate pH value and is 6.8, and centrifugal, the collection supernatant gets 11.3kg; Carry out coarse filtration with plate filter, collect filter liquor and get 11.1kg; With water for injection with DEAE furnishing pasty state; Adopt slurry type method dress post; Dress column pressure 0.5Mpa, using 400mmol/L pH value behind the dress post earlier is 6.8 phosphate buffer cleaning, using 15mmol/L pH value again is 6.9 phosphate buffer balance; DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and use 15mmol/L pH value is 6.9 phosphate buffer wash-out.Collect object and get 10.96kg, use molecular cut off to carry out ultrafiltration, collect ultrafiltrate 10.85kg, obtain the jackfruit extract as the milipore filter of 90KD.
Gained jackfruit extract is detected, and the result sees table 5.
Table 5 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1019 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (forint phenol method), mg/100mL | 527 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 7.00 |
| Water soluble starch, mg/100mL | 78 |
| Cellulose, mg/100mL | 16 |
| Vitamin, mg/100mL | 12 |
| Ca，％ | 0.0325 |
| Mg，％ | 0.0192 |
| Na，％ | 0.0044 |
| Zn，％ | 0.0041 |
Embodiment 6
Choose one of ripe jackfruit fruit, 9.5kg weighs; Get luxuriant meat, seed after cutting open, seed is soaked heated and boiled 5min behind the 30min with 0.5% sodium acid carbonate, 85 ℃ of baking 1h, the cooling back is rubbed with the hands and is removed the seed epidermis, and mixing obtains raw material with seed with luxuriant meat, is weighed as 4.7kg; Add 9.42L distilled water, distilled water is added in the making beating back, obtains stoste 13.01L, is weighed as 13.42kg; Stoste is warming up to 47 ℃; With 0.1mol/L NaOH stoste is regulated pH value to 8.7,, in stoste, add the pancreatin of 28.2 ten thousand IU according to the pancreatin of every kg raw material (gross weight of luxuriant meat and seed) interpolation 60,000 IU; After mixing; In the pH value is 8.7, and temperature is enzymolysis 4h under 47 ℃ the condition, obtains first enzymolysis liquid; With 0.1mol/LHCl the pH value of first enzymolysis liquid is adjusted to 4.2 again; Add cellulase and the papain of 150,000 IU respectively according to every kg raw material (gross weight of luxuriant meat and seed); In stoste, adding the cellulase of 70.6 ten thousand IU and the papain of 70.6 ten thousand IU respectively, after mixing, is 4.2 in the pH value; Temperature is enzymolysis 3h under 47 ℃ the condition, obtains second enzymolysis liquid; After enzymolysis finished, with stoste heat temperature raising to 100 ℃, insulation 10min carried out enzyme-deactivating, is cooled to below 30 ℃, uses 0.1mol/L NaOH to regulate pH value and is 7.2, and centrifugal, the collection supernatant gets 9.89kg; Carry out coarse filtration with plate filter, collect filter liquor and get 9.76kg; With water for injection with DEAE furnishing pasty state; Adopt slurry type method dress post; Dress column pressure 0.5Mpa, using 400mmol/L pH value behind the dress post earlier is 7.0 phosphate buffer cleaning, using 20mmol/L pH value again is 7.0 phosphate buffer balance; DEAE chromatographic column on the above-mentioned filter liquor is adsorbed, and use 20mmol/L pH value is 7.0 phosphate buffer wash-out.Collect object and get 9.66kg, use molecular cut off to carry out ultrafiltration, collect ultrafiltrate 9.48kg, obtain the jackfruit extract as the milipore filter of 100KD.
Gained jackfruit extract is detected, and the result sees table 6.
Table 6 jackfruit extract testing result
| Test item | Testing result |
| Polysaccharide is differentiated (phenolsulfuric acid reagent reacting) | Positive |
| Polyoses content (phenolsulfuric acid method), mg/100mL | 1015 |
| Polypeptide is differentiated (ninhydrin method) | Positive |
| Content of peptides (forint phenol method), mg/100mL | 515 |
| PH value (2005 editions second appendix VI H of Chinese Pharmacopoeia) | 6.95 |
| Water soluble starch, mg/100mL | 82 |
| Cellulose, mg/100mL | 12 |
| Vitamin, mg/100mL | 12 |
| Ca，％ | 0.0334 |
| Mg，％ | 0.0178 |
| Na，％ | 0.0042 |
| Zn，％ | 0.0043 |
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (7)
1. a jackfruit processing method is characterized in that, comprising:
Step 1: the seed of jackfruit is soaked 30min with 0.5～1% sodium bicarbonate solution, remove epidermis after the heating cooling, clean the oven dry back and mix with the luxuriant meat of jackfruit and obtain raw material, in raw material, add entry and make stoste;
Step 2: in said stoste, adding alkali, to make the pH value of said stoste be 8.3～8.7, under 43～47 ℃, adds the pancreatin of 1.0～10.0 ten thousand IU in every kg raw material, and enzymolysis 2～6h obtains first enzymolysis liquid;
Step 3: in said first enzymolysis liquid, adding acid, to make the pH value of said first enzymolysis liquid be 3.8～4.2; Under 43～47 ℃; Add in the AMS, pectase, cellulase, carbohydrase, papain of 1.0～20.0 ten thousand IU one or more in every kg raw material; Enzymolysis 2～6h obtains second enzymolysis liquid;
Step 4: said second enzymolysis liquid is made the jackfruit extract after the separation of DEAE chromatographic column, ultrafiltration.
2. the method for claim 1 is characterized in that, in g/mL, the mass volume ratio of raw material and water is 1: 2～8.
3. the method for claim 1 is characterized in that, said DEAE chromatographic column separates employing 10～30mmol/L, and the pH value is 6.8～7.2 phosphate buffer.
4. the method for claim 1 is characterized in that, it is the milipore filter of 50～100KD that molecular cut off is adopted in said ultrafiltration.
5. the method for claim 1 is characterized in that, said DEAE chromatographic column also comprises filtration, centrifugation step before separating.
6. the method for claim 1 is characterized in that, said alkali is one or more the mixed base in NaOH, calcium hydroxide, potassium hydroxide, sodium acid carbonate, the ammoniacal liquor.
7. the method for claim 1 is characterized in that, said acid is one or more the mixed acid in hydrochloric acid, sulfuric acid, phosphoric acid, the citric acid.
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| CN103710214A (en) * | 2014-01-06 | 2014-04-09 | 中国热带农业科学院香料饮料研究所 | Jack fruit wine and preparation method thereof |
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| CN105063129A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Ultrasonic extraction process of kudzuvine root fructose |
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| CN112971133A (en) * | 2021-04-19 | 2021-06-18 | 海南云皓生物科技有限公司 | Paederia scandens enzyme, preparation method thereof and application of paederia scandens enzyme in preparation of anti-inflammatory and analgesic products |
| CN114098075A (en) * | 2021-11-09 | 2022-03-01 | 安徽盛美诺生物技术有限公司 | Jackfruit-based compound preparation with immunocompetence and application thereof |
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