CN102297857A - Intelligent red blood cell folate detector and detection method - Google Patents
Intelligent red blood cell folate detector and detection method Download PDFInfo
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- CN102297857A CN102297857A CN2011102234575A CN201110223457A CN102297857A CN 102297857 A CN102297857 A CN 102297857A CN 2011102234575 A CN2011102234575 A CN 2011102234575A CN 201110223457 A CN201110223457 A CN 201110223457A CN 102297857 A CN102297857 A CN 102297857A
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Abstract
The invention discloses an intelligent red blood cell folate detector and a detection method. The intelligent red blood cell folate detector comprises a measuring system for collecting photons released by a sample needing to be detected, a liquid feeding pump system for filling a reagent into a sample tube, a photon counting system for counting the number of photons, and a control system comprising a power circuit, a single-chip microcomputer, a keyboard and a display, wherein the keyboard and the display are connected to the single-chip microcomputer. The detection method comprises the following steps of putting the sample tube on a detection site in a measuring chamber, filling a reagent into the sample tube by the liquid feeding pump system, collecting photon signals sent out by a photomultiplier tube (PMT) to counting the number of photons, and under control of the single-chip microcomputer, calculating a red blood cell folate content value and displaying directly the red blood cell folate content value on the display. The intelligent red blood cell folate detector has the advantages that the intelligent red blood cell folate detector can show directly a red blood cell folate content value of a patient, does not need the help of radioactive substances, can be operated easily, has high sensitivity and low costs, can produce stable detection results, and most mainly, does not produce radioactive pollution.
Description
Technical field
The present invention relates to a kind of field of medical technology, the method for particularly a kind of intelligent red blood cell acidum folicum detector and detection.
Background technology
For a long time, generally mensuration in the clinical examination to folate content in the human serum, the shortage of judging human body folic acid is whether.In recent years, prove that after deliberation folate content in the red blood cell is 10 ~ 20 times of folate content in the serum, and folate content is owing to be subjected to the influence of foeign elements such as diet, disease in the serum, its concentration level fluctuation is bigger.And the interference that folate content is subjected to extraneous factor in the red blood cell seldom, more can reflect the concentration level of folic acid in the tissue truly.But the method and the clinical detection instrument that also do not have direct quantitative human body red blood cell acidum folicum content up till now both at home and abroad.Only measure serum folic acid content and can not satisfy clinical medical requirement, find that especially the height of red blood cell acidum folicum level has very important value to prevention population inborn defect, the work of measuring red blood cell acidum folicum content just has a kind of new meaning.
Past serum folic acid assay generally adopts radio-immunity detection method γ-calculating instrument to carry out.This technology (be called for short (RIA)) is a kind of microanalysis method that radioisotope labeling and antigen-antibody reaction are combined.
The classical RIA that nineteen sixty sets up makes isotope-labeled antigen and unlabelled antigen to be measured and corresponding antibody effect, under the condition of antigen excess, two kinds of antigens combine with antibody competition, try to achieve the content of determined antigen then by the radioactivity of measuring antigen antibody complex and free antigen.This method determination step is loaded down with trivial details, the reaction time long, reagent stability is poor, the more important thing is because label is a radioactive isotope, big for environment pollution, people's health there is threat, but owing to relate to methodological characteristics, speciality circles can only do something against one's will in a suitable period.
In recent years because the appearance of chemical luminescence detection method, this method is highly sensitive, accuracy good, practical stability, especially do not have environmental pollution, be suitable for, but chemiluminescence method also of no use at present detects the equipment of red blood cell acidum folicum content in the technical application of quantitative detection of folic acid.
Summary of the invention
One of the object of the invention provides a kind of chemoluminescence method that is applicable to detect the intelligent red blood cell acidum folicum detector of red blood cell acidum folicum content.
To achieve these goals, the present invention is by the following technical solutions:
A kind of intelligent red blood cell acidum folicum detector comprises: measuring system, and comprise PMT and reach and the airtight measuring chamber that is used to place specimen tube that is connected of PMT, be used for the photon that the acquisition testing sample discharges; The fluid filling pump system links to each other with measuring chamber, is used for to specimen tube filling reagent; Photo Counting System comprises high-tension circuit module, division module, the pulse module sum counter module of electrical connection, is used for photon is counted; And control system, comprise power circuit, have the single-chip microcomputer and keyboard that links to each other with single-chip microcomputer and the display that calculate red blood cell acidum folicum range preface; Wherein measuring chamber comprises casing and door.
In the embodiment of the invention, be provided with in the measuring chamber and be used for the detection optoelectronic switch whether specimen tube is put into, the control corresponding system includes the specimen tube testing circuit.
In the embodiment of the invention, a pair of door magnetic that is used for the folding condition of control door is installed on casing and the door, the control corresponding system includes a testing circuit.
In the embodiment of the invention, the fluid filling pump system comprises holder, be installed in motor on the holder, by cam and code-disc, two guide poles and two corrugated tubes of driven by motor, each guide pole one end contacts with cam face, the other end connects corrugated tube one end, the corrugated tube other end is equipped with solenoid valve, each corrugated tube all is set with coil tension spring outward, and the control corresponding system includes pump control circuit.
In the embodiment of the invention, measuring chamber also comprises automatic door operator, automatic door operator comprises the door bolt that has spring that is movably connected on the door, what cooperate with door bolt is arranged on guide on the cabinet wall, the mount pad that the outer inside of casing has electromagnet is set, is arranged on the coil brace on the mount pad and the interior coil of coil brace is set.
In the embodiment of the invention, PMT, high-tension circuit module, division module, pulse module are installed in the metallic shield box.
In the embodiment of the invention, it also comprises the printer that links to each other with single-chip microcomputer.
Two of the object of the invention provides a kind of and utilizes above-mentioned intelligent red blood cell acidum folicum detector to carry out the method that red blood cell acidum folicum detects.
To achieve these goals, the present invention is by the following technical solutions:
A kind of method of utilizing described intelligent red blood cell acidum folicum detector to carry out the detection of red blood cell acidum folicum amount may further comprise the steps:
1) specimen tube is put into the detecting position of measuring chamber, then manual-lock measuring chamber door;
2) the fluid filling pump system is to the specimen tube reagent of annotating; Simultaneously, high-tension circuit module and division module are sent to PMT and are measured instruction and the measurement high pressure is provided;
3) PMT gathers the photon signal that sends, and by division module, pulse module sum counter module photon is counted;
4) under the control of single-chip microcomputer, manually import patient's packed cell volume value by keyboard, by the calculating of single-chip microcomputer, can directly on display, show patient's red blood cell acidum folicum content value.
Beneficial effect of the present invention:
The present invention gathers the photon number of using the chemoluminescence method test sample to discharge by PMT, carry out photon counting by number system, under the control of single-chip microcomputer, input patient's packed cell volume value, promptly can directly report out patient's red spire acid content, not by radiomaterial, instrumentation is simple, highly sensitive, testing result stable, the present invention is with low cost, and main is not have radioactive contamination.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 is the structural representation of measuring chamber.
Fig. 3 is the structural representation of fluid filling pump system.
Among the figure 1, Photo Counting System, 2, measuring system, 3, control system, 4, door magnetic, 5, casing, 6, mount pad, 7, coil brace, 8, coil, 9, guide, 10, detect optoelectronic switch, 11, door bolt, 12, spring, 13, door, 14, solenoid valve, 15, motor, 16, coil tension spring, 17, holder, 18, corrugated tube, 19, guide pole, 20, cam, 21, code-disc.
Embodiment
Better understand the present invention for the ease of those skilled in the art, below in conjunction with the drawings and specific embodiments the present invention is described in further details, following explanation only is exemplary, does not limit protection scope of the present invention.
With reference to the accompanying drawings 1, a kind of intelligent red blood cell acidum folicum detector, comprise the measuring system 2 that is used for the photon that the acquisition testing sample discharges, be used for to specimen tube filling reagent the fluid filling pump system, be used for Photo Counting System 1 and control system 3 that photon is counted.Measuring system 2 comprises PMT and reaches and the airtight measuring chamber that is used to place specimen tube that is connected of PMT, and the PMT probe should be over against the detection hole of sample in the practice, and measuring chamber comprises casing 5 and door 13; The fluid filling pump system links to each other with measuring chamber; Photo Counting System 1 comprises high-tension circuit module, division module, the pulse module sum counter module of electrical connection; Control system 3 comprises: power circuit, charging circuit has the single-chip microcomputer that calculates red blood cell acidum folicum range preface, and the keyboard that links to each other with single-chip microcomputer, printer, reaches display.
With reference to the accompanying drawings 2, when needing to put into specimen tube in the practice, measuring chamber can open to have simultaneously to detect whether specimen tube is put into and the function of measuring chamber door folding condition automatically.Measuring chamber is opened the dependence automatic door operator automatically, automatic door operator comprises the door bolt that has spring 12 11 that is movably connected on the door 15, what cooperate with door bolt 11 is arranged on guide 9 on casing 5 inwalls, the mount pad 6 that casing 5 outer inside have electromagnet is set, is arranged on coil brace 7 and the coil 8 that is provided with in the coil brace 7 on the mount pad 6; Be provided with in the measuring chamber and be used for the detection optoelectronic switch 10 whether specimen tube is put into, control corresponding system 3 includes the specimen tube testing circuit; The a pair of door magnetic 4 that is used for the folding condition of control door is installed on casing 5 and the door 13, and control corresponding system 3 includes a testing circuit.
With reference to the accompanying drawings 3, the cam 20 that the fluid filling pump system comprises pump control circuit, holder 17, be installed in motor 15 on the holder 17, driven by motor 15 and code-disc 21, two guide poles 19 and two corrugated tubes 18, each guide pole 19 1 end contacts with cam 20 surfaces, the other end connects corrugated tube 18 1 ends, corrugated tube 18 other ends are equipped with solenoid valve 14, all be set with coil tension spring 16 outside each corrugated tube 18, control corresponding system 3 includes pump control circuit.The fluid filling pump system work process is as follows: under the drive of motor 15, cam 20 and code-disc 21 rotate with motor 15, and then drive guide pole 19 along upwards slip of cam 20, guide pole 19 and coil tension spring 2 are together, it is flexible to drive corrugated tube 18, under the control of single-chip microcomputer, when corrugated tube 18 elongates, the inlet of solenoid valve 14 is opened, and liquid outlet is closed; When corrugated tube 18 shrank, the inlet of solenoid valve 14 was closed, and liquid outlet is opened. by measuring the amount that code-disc 21 rotates, control flexible what of corrugated tube 18, to realize accurate liquid feeding.
In order to guarantee test result accuracy and stability, PMT, high-tension circuit module, division module and pulse module are installed in the metallic shield box.
A kind of method of utilizing described intelligent red blood cell acidum folicum detector to carry out the detection of red blood cell acidum folicum amount may further comprise the steps:
1) by automatic automatic door operator the door 13 of measuring chamber is opened, specimen tube is put into the detecting position of measuring chamber, then the manual-lock measuring chamber the door 13, whether detect optoelectronic switch 10 and specimen tube testing circuit this moment and cooperate the detection specimen tube to put into, whether door magnetic 4 cooperates detecting gate 13 to close with a door testing circuit;
2) if above-mentioned situation is normal, pump control circuit control fluid filling pump system is to the specimen tube reagent of annotating; Simultaneously, high-tension circuit module and division module are sent to PMT and are measured instruction and the measurement high pressure is provided;
3) PMT gathers the photon signal that sends, and by division module, pulse module sum counter module photon is counted;
4) under the control of single-chip microcomputer, manually import patient's packed cell volume value by keyboard, by the calculating of single-chip microcomputer 8, can directly on display, show and/or the red blood cell acidum folicum content value by the printer prints patient.
Above embodiment only is used to illustrate that technical scheme of the present invention is not intended to limit; although the present invention is had been described in detail by embodiment; under the field personnel should make amendment or the part technical characterictic is equal to replacement with reference to concrete mode of the present invention; but under the spirit that does not break away from technical solution of the present invention, above-mentioned change or be equal to replacement and should belong in the technical scheme scope that the present invention asks for protection.
Claims (8)
1. an intelligent red blood cell acidum folicum detector is characterized in that: comprise
Measuring system (2) comprises PMT and reaches and the airtight measuring chamber that is used to place specimen tube that is connected of PMT, is used for the photon that the acquisition testing sample discharges;
The fluid filling pump system links to each other with measuring chamber, is used for to specimen tube filling reagent;
Photo Counting System (1) comprises the high-tension circuit module, division module, the pulse module sum counter module that electrically connect, is used for photon is counted; With
Control system (3) comprises power circuit, has the single-chip microcomputer and keyboard that links to each other with single-chip microcomputer and the display that calculate red blood cell acidum folicum range preface;
Wherein measuring chamber comprises casing (5) and door (13).
2. according to the described intelligent red blood cell acidum folicum detector of claim 1, it is characterized in that: be provided with in the measuring chamber and be used for the detection optoelectronic switch whether specimen tube is put into, control corresponding system (3) includes the specimen tube testing circuit.
3. according to the described intelligent red blood cell acidum folicum detector of claim 1, it is characterized in that: a pair of door magnetic (4) that is used for the folding condition of control door is installed on casing (5) and the door (13), and control corresponding system (3) includes a testing circuit.
4. according to the described intelligent red blood cell acidum folicum detector of claim 1, it is characterized in that: the fluid filling pump system comprises holder (17), be installed in the motor (15) on the holder (17), cam (20) and code-disc (21) by motor (15) drive, two guide poles (19) and two corrugated tubes (18), each guide pole (19) one end contacts with cam (20) surface, the other end connects corrugated tube (18) one ends, corrugated tube (18) other end is equipped with solenoid valve (14), each corrugated tube (18) is outer all to be set with coil tension spring (16), and control corresponding system (3) includes pump control circuit.
5. according to the described intelligent red blood cell acidum folicum detector of claim 1, it is characterized in that: measuring chamber also comprises automatic door operator, automatic door operator comprises the door bolt that has spring (12) (11) that is movably connected on the door (15), what cooperate with door bolt (11) is arranged on guide (9) on casing (5) inwall, the mount pad (6) that the outer inside of casing (5) has electromagnet is set, is arranged on the coil brace (7) on the mount pad (6) and the interior coil of coil brace (7) is set.
6. according to any described intelligent red blood cell acidum folicum detector of claim 1 to 5, it is characterized in that: PMT, high-tension circuit module, division module, pulse module are installed in the metallic shield box.
7. according to any described intelligent red blood cell acidum folicum detector of claim 1 to 5, it is characterized in that: it also comprises the printer that links to each other with single-chip microcomputer.
8. one kind is utilized the described intelligent red blood cell acidum folicum detector of claim 1 to carry out the method that the red blood cell acidum folicum amount detects, and it is characterized in that: may further comprise the steps
1) specimen tube is put into the detecting position of measuring chamber, then manual-lock measuring chamber door;
2) the fluid filling pump system is to the specimen tube reagent of annotating; Simultaneously, high-tension circuit module and division module are sent to PMT and are measured instruction and the measurement high pressure is provided;
3) PMT gathers the photon signal that sends, and by division module, pulse module sum counter module photon is counted;
4) under the control of single-chip microcomputer, manually import patient's packed cell volume value by keyboard, by the calculating of single-chip microcomputer, can directly on display, show 1 patient's red blood cell acidum folicum content value.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011102234575A CN102297857A (en) | 2011-08-05 | 2011-08-05 | Intelligent red blood cell folate detector and detection method |
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| CN2011102234575A CN102297857A (en) | 2011-08-05 | 2011-08-05 | Intelligent red blood cell folate detector and detection method |
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| CN103149132A (en) * | 2013-03-18 | 2013-06-12 | 深圳市理邦精密仪器股份有限公司 | Detection system for improving accuracy of measuring packed cell volume accuracy and operating control method |
| CN113376186A (en) * | 2021-05-12 | 2021-09-10 | 赵静 | High-precision intelligent erythrocyte folic acid detector and detection method thereof |
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| CN113376186A (en) * | 2021-05-12 | 2021-09-10 | 赵静 | High-precision intelligent erythrocyte folic acid detector and detection method thereof |
| CN113376186B (en) * | 2021-05-12 | 2022-12-23 | 无锡科金生物科技有限公司 | High-precision intelligent erythrocyte folic acid detector and detection method thereof |
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Application publication date: 20111228 |