CN102286788B - Method for degumming flax by using microbial rain and dew - Google Patents
Method for degumming flax by using microbial rain and dew Download PDFInfo
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- CN102286788B CN102286788B CN 201110167644 CN201110167644A CN102286788B CN 102286788 B CN102286788 B CN 102286788B CN 201110167644 CN201110167644 CN 201110167644 CN 201110167644 A CN201110167644 A CN 201110167644A CN 102286788 B CN102286788 B CN 102286788B
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Abstract
The invention provides a method for degumming flax by using microbial rain and dew and relates to a method for degumming flax by using rain and dew. The invention aims to solve the problems of low long hemp rate and overlong degumming time in the conventional method for degumming flax by using rain and dew. The method comprises the following steps of: performing fermenting cultivation on two strains of Bacillus subtilis YT1 and YT2 to obtain two kinds of bacterial solution; mixing the bacterial solution; adding an H-901 penetrating agent to prepare mixed microbial inoculum; spraying the microbial inoculum into the flax to be dewed to degum the flax by using rain and dew. By the method for degumming the flax by using rain and dew, the degumming time is shortened to 8 to 15 days. In the traditional process, the degumming time is 30 to 45 days, so the degumming time in the method is shortened by over 50 percent. After hemp is prepared from the degummed flax stems, the average hemp rate is increased by 2 percent and the comprehensive hemp rate is increased by 1 percent. The method is applicable to large-scale popularization of retting by rain and dew.
Description
Technical field
The present invention relates to flax rain and dew Degumming method.
Background technology
Flax degumming is an important link of flax process, directly affects the index such as long flax rate, intensity, division degree of flax.At present, external main produce numb state and replaced the warm water degumming tech such as French, Belgian etc. being come unstuck by rain and dew fully because of factors such as environmental protection, the warm water retting technology only has a few countries such as China, Egypt at present still in employing.
But the characteristics of rain and dew retted fibre technique are little, the non-environmental-pollution continuous mechanized operation of investment etc. makes it replace warm water retting technique fully in Europe, and its products characteristics is that intensity is good.Rain and dew retted fibre technique is subjected to the restriction of natural conditions very large, and such as the main growing area in the Europe such as France, natural conditions are maritime climate, and with the humid air, crinosity drizzle weather, temperature are suitable, and rain is crossed both sunny, and temperature is not high, soil is permeable soil.Such climate because the alternating action of rainwater and sunlight has been arranged, and is aided with flax turning machine repeatedly turning over of falx straw is dried in the air, and has guaranteed that retted fibre is even, just right.And there are the large and problem such as occupied ground is large, labor strength is large, the amount of labour used is large and scutched line intensity is low of quantity of wastewater effluent in warm water retting technique and dew retting ratio.
In recent years, according to expert advice, the flax planting district moves northward, but is subjected to the restriction of objective condition, does not have retted fibre pond and the airing place of warm water retting, and this has also promoted the development of rain and dew retted fibre industry.But because Bei'an and northern area receipts fiber crops rainfall in season and dew retting are to the restriction of the factors such as requirement of temperature; therefore; the unsettled phenomenon of rain and dew retted fibre quality in autumn often can appear; even can the retted fibre process time is long maybe can not be finished because the rainfall deficiency occurs; cause long flax rate low, thereby cause the problem of the cheap and dull sale of product.But the rain and dew retted fibre is the developing direction of flax degumming, in order to comply with this developing direction, needs the problem that method that a kind of new flax rain and dew come unstuck overcomes the low and technical process overlong time of the long flax rate that exists in the conventional method badly.
Summary of the invention
The present invention will solve the problem that long flax rate is low and usually time is long that exists in existing flax rain and dew Degumming method, and the method that provides the flax microorganism rain and dew to come unstuck.
The method that flax microorganism rain and dew of the present invention come unstuck, realize by following steps: one, two bacillus subtilis (Bacillus subtilis) YT1 and YT2 are placed on respectively in the fermentation tank and cultivated 48 hours, cultivation temperature is 37～40 ℃, pH is 8～9, and the amount that obtains containing two bacillus subtilis YT1 and YT2 is 10
13Two kinds of bacterium liquid of individual/ml; Wherein, the YT1 preserving number is CCTCC M 2011165, and the YT2 preserving number is CCTCC M 2011166; Two, the YT1 that obtains in the step 1 and two kinds of bacterium liquid of YT2 are mixed by 1: 0.7～1.5 volume ratio after, thin up is respectively 1% and 0.7%～1.5% to the volume fraction that contains YT1 and two kinds of bacterium of YT2, adds the H-901 bleeding agent again, obtains mix bacterium agent; Wherein, the volume fraction that contains the H-901 bleeding agent in the mix bacterium agent is 0.1%～0.5%; Three, utilize spraying machine that the mix bacterium agent that obtains in the step 2 is sprayed on flax stem to be revealed, the temperature that controls environment is at 15～40 ℃, and the sprinkling amount is per hectare 600～900kg, finishes the flax microorganism rain and dew and comes unstuck.
The H-901 bleeding agent is the commercially available prod in the step 2 of the present invention, is the H-901 high-efficient penetrant that is used for flax product by the specialty of Heilongjiang Province Chemical Industry Research Institute's development and production.
The method that flax microorganism rain and dew of the present invention come unstuck by add two kinds of bacillus subtilis (Bacillus subtilis) YT1 and YT2 and H-901 bleeding agent in mix bacterium agent, reaches the raising long flax rate, and the purpose that shortens usually time.YT1 of the present invention mainly produces alkaline pectase, YT2 mainly produces hemicellulase, decompose non-cellulose composition pectin and hemicellulose in flax, the flax that adds simultaneously 0.1%H-901 promotes bacterium liquid to the wet processes of flax straw with bleeding agent, thereby in the time shorten to 15 that will come unstuck day, compares with traditional handicraft, usually time shortens more than 50%, and behind flax stem fiber crops processed after will coming unstuck, its average long flax rate improves 2%, and comprehensive numb rate improves 1%.The present invention is applicable to the large-scale promotion of rain and dew retted fibre.
Two bacillus subtilis used in the present invention (Bacillus subtilis) YT1 and YT2, provide preservation to Chinese Typical Representative culture collection center (CCTCC), preservation address: Wuhan City Wuhan University, preservation date: on May 10th, 2011, the YT1 preserving number is CCTCC No:M 2011165, and the YT2 preserving number is CCTCC No:M 2011166.
The specific embodiment
The specific embodiment one: the method that the flax microorganism rain and dew come unstuck in the present embodiment, realize by following steps: one, two bacillus subtilis (Bacillus subtilis) YT1 and YT2 are placed on respectively in the fermentation tank and cultivated 48 hours, cultivation temperature is 37～40 ℃, pH is 8～9, and the amount that obtains containing two bacillus subtilis YT1 and YT2 is 10
13Two kinds of bacterium liquid of individual/ml; Wherein, the YT1 preserving number is CCTCC M2011165, and the YT2 preserving number is CCTCC M 2011166; Two, the YT1 that obtains in the step 1 and two kinds of bacterium liquid of YT2 are mixed by 1: 0.7～1.5 volume ratio after, thin up is respectively 1% and 0.7%～1.5% to the volume fraction that contains YT1 and two kinds of bacterium of YT2, adds the H-901 bleeding agent again, obtains mix bacterium agent; Wherein, the volume fraction that contains the H-901 bleeding agent in the mix bacterium agent is 0.1%～0.5%; Three, utilize spraying machine that the mix bacterium agent that obtains in the step 2 is sprayed on flax stem to be revealed, the temperature that controls environment is at 15～40 ℃, and the sprinkling amount is per hectare 600～900kg, finishes the flax microorganism rain and dew and comes unstuck.
Two bacillus subtilis YT1 separate according to the following steps with YT2 and obtain in the present embodiment step 1: be sub-packed in the Boiling tube after the rain and dew straw section of being cut into flax, it is the 0.85%NaCl aqueous solution that each Boiling tube adds mass fraction, the solid-liquid mass ratio is 1: 10～15, be heated to 65～90 ℃, keep this temperature 20～40min, then be coated on the ordinary culture medium, single bacterium colony of growth is transferred on the isolation medium, incubated overnight, then with the full copper acetate dyeing 15min that closes, choose the bacterium colony that transparent circle is arranged, obtain two bacillus subtilis YT1 and YT2; Wherein, ordinary culture medium is beef extract 1%～1.5% by mass percentage, peptone 0.8%～1.2%, yeast extract 0.3%～0.5%, sodium chloride 0.4%～0.6%; Isolation medium is pectin 0.5%～1.2% by mass percentage, peptone 0.1%～0.5%, yeast extract 0.1%～0.3%, agar powder 0.8%-1.2%; Wherein, the rain and dew straw of flax is from flax institute of grand celebration branch of Heilongjiang Prov. Academy of Sciences.
The fermentation cylinder for fermentation culture medium is by mass percentage mainly by corn flour 6% in the present embodiment step 1, bean powder 4%, Na
2HPO
40.4%, NaH
2PO
40.03%, Na
2CO
30.1% forms;
The H-901 bleeding agent is the commercially available prod in the present embodiment step 2, is the H-901 high-efficient penetrant that is used for flax product by the specialty of Heilongjiang Province Chemical Industry Research Institute's development and production;
YT1 in the present embodiment step 1 mainly produces alkaline pectase, YT2 mainly produces hemicellulase, decompose non-cellulose composition pectin and hemicellulose in flax, the flax that adds simultaneously 0.1%H-901 promotes bacterium liquid to the wet processes of flax straw with bleeding agent, thereby in the time shorten to 15 that will come unstuck day, compares with traditional handicraft, usually time shortens more than 50%, and behind flax stem fiber crops processed after will coming unstuck, its average long flax rate improves 2%, and comprehensive numb rate improves 1%.
The specific embodiment two: what present embodiment and the specific embodiment one were different is that cultivation temperature is 38 ℃ in the step 1, and pH is 8.5.Other step and parameter are identical with the specific embodiment one.
The specific embodiment three: present embodiment and the specific embodiment one to two are different is that thin up is respectively 1% and 0.8～1.4% to the volume fraction that contains YT1 and two kinds of bacterium of YT2 after in the step 2 YT1 that obtains in the step 1 and two kinds of bacterium liquid of YT2 being mixed by 1: 0.8～1.4 volume ratio.Other step and parameter are identical with the specific embodiment one to two.
The specific embodiment four: present embodiment and the specific embodiment one to two are different is that thin up is respectively 1% and 1% to the volume fraction that contains YT1 and two kinds of bacterium of YT2 after in the step 2 YT1 that obtains in the step 1 and two kinds of bacterium liquid of YT2 being mixed by 1: 1 volume ratio.Other step and parameter are identical with the specific embodiment one to two.
The specific embodiment five: present embodiment and the specific embodiment one to four are different is that the volume fraction that contains the H-901 bleeding agent in the step 2 in the mix bacterium agent is 0.2%～0.4%.Other step and parameter are identical with the specific embodiment one to four.
The specific embodiment six: present embodiment and the specific embodiment one to four are different is that the volume fraction that contains the H-901 bleeding agent in the step 2 in the mix bacterium agent is 0.3%.Other step and parameter are identical with the specific embodiment one to four.
The specific embodiment seven: what present embodiment and the specific embodiment one to six were different is to control environment temperature in the step 3 at 20～35 ℃, and the sprinkling amount is per hectare 650～850kg.Other step and parameter are identical with the specific embodiment one to six.
The specific embodiment eight: what present embodiment and the specific embodiment one to six were different is to control environment temperature in the step 3 at 30 ℃, and the sprinkling amount is per hectare 700kg.Other step and parameter are identical with the specific embodiment one to six.
The specific embodiment nine: the method that the flax microorganism rain and dew come unstuck in the present embodiment, realize by following steps: one, two bacillus subtilis (Bacillus subtilis) YT1 and YT2 are placed on respectively in the fermentation tank and cultivated 48 hours, cultivation temperature is 38 ℃, pH is 8.2, and the amount that obtains containing two bacillus subtilis YT1 and YT2 is 10
13Two kinds of bacterium liquid of individual/ml; Wherein, the YT1 preserving number is CCTCC M 2011165, and the YT2 preserving number is CCTCC M 2011166; Two, the YT1 that obtains in the step 1 and two kinds of bacterium liquid of YT2 are mixed by 1: 1 volume ratio after, thin up is respectively 1% and 1% to the volume fraction that contains YT1 and two kinds of bacterium of YT2, adds the H-901 bleeding agent again, obtains mix bacterium agent; Wherein, the volume fraction that contains the H-901 bleeding agent in the mix bacterium agent is 0.1%; Three, utilize spraying machine that the mix bacterium agent that obtains in the step 2 is sprayed on flax stem to be revealed, the temperature that controls environment is at 20 ℃, and the sprinkling amount is per hectare 700kg, finishes the flax microorganism rain and dew and comes unstuck.
The fermentation cylinder for fermentation culture medium is by mass percentage mainly by corn flour 6% in the present embodiment step 1, bean powder 4%, Na
2HPO
40.4%, NaH
2PO
40.03%, Na
2CO
30.1% forms;
The H-901 bleeding agent is the commercially available prod in the present embodiment step 2, is the H-901 high-efficient penetrant that is used for flax product by the specialty of Heilongjiang Province Chemical Industry Research Institute's development and production;
The time shorten to 10 that the method that the flax microorganism rain and dew of present embodiment come unstuck will be come unstuck day, and behind flax stem fiber crops processed after will coming unstuck, its long flax rate is 12.3%, comprehensive numb rate is 23.4%.And behind flax stem fiber crops processed that the existing flax rain and dew Degumming method of use comes unstuck, its long flax rate is 9.8%, and comprehensive numb rate is 22.1%.
Claims (6)
1. the flax microorganism rain and dew method of coming unstuck, it is characterized in that the method that the flax microorganism rain and dew come unstuck mainly may further comprise the steps: one, two bacillus subtilis (Bacillus subtilis) YT1 and YT2 are placed on respectively in the fermentation tank and cultivated 48 hours, cultivation temperature is 37～40 ℃, pH is 8～9, and the amount that obtains containing two bacillus subtilis YT1 and YT2 is 10
13Two kinds of bacterium liquid of individual/ml; Wherein, the YT1 preserving number is CCTCC M 2011165, and the YT2 preserving number is CCTCC M2011166; Two, the YT1 that obtains in the step 1 and two kinds of bacterium liquid of YT2 are mixed by 1: 0.7～1.5 volume ratio after, thin up is respectively 1% and 0.7%～1.5% to the volume fraction that contains YT1 and two kinds of bacterium of YT2, adds the H-901 bleeding agent again, obtains mix bacterium agent; Wherein, the volume fraction that contains the H-901 bleeding agent in the mix bacterium agent is 0.1%～0.5%; Three, utilize spraying machine that the mix bacterium agent that obtains in the step 2 is sprayed on flax stem to be revealed, the temperature that controls environment is at 15～40 ℃, and the sprinkling amount is per hectare 600～900kg, finishes the flax microorganism rain and dew and comes unstuck.
2. the flax microorganism rain and dew according to claim 1 method of coming unstuck is characterized in that cultivation temperature is 38 ℃ in the step 1, and pH is 8.5.
3. the flax microorganism rain and dew according to claim 1 and 2 method of coming unstuck, after it is characterized in that in the step 2 that the YT1 that will obtain in the step 1 and two kinds of bacterium liquid of YT2 mix by 1: 0.8～1.4 volume ratio, thin up is respectively 1% and 0.8～1.4% to the volume fraction that contains YT1 and two kinds of bacterium of YT2.
4. the flax microorganism rain and dew according to claim 3 method of coming unstuck, after it is characterized in that in the step 2 that the YT1 that will obtain in the step 1 and two kinds of bacterium liquid of YT2 mix by 1: 1 volume ratio, thin up is respectively 1% and 1% to the volume fraction that contains YT1 and two kinds of bacterium of YT2.
5. the flax microorganism rain and dew according to claim 4 method of coming unstuck, the volume fraction that it is characterized in that containing in the mix bacterium agent in the step 2 H-901 bleeding agent is 0.2%～0.4%.
6. the flax microorganism rain and dew according to claim 5 method of coming unstuck, the temperature that it is characterized in that controling environment in the step 3 is at 20～35 ℃, and the sprinkling amount is per hectare 650～850kg.
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| CN104250858B (en) * | 2014-07-14 | 2016-06-08 | 东华大学 | A kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare bastose |
| CN108823192A (en) * | 2018-06-08 | 2018-11-16 | 山东宸青生物科技有限公司 | The production method of crudefiber crop fiber refining enzyme |
| CN109112691A (en) * | 2018-09-03 | 2019-01-01 | 哈尔滨华仁亚麻纺织有限公司 | The production technology of the easypro elater scribbled of flax |
| CN111379034A (en) * | 2020-04-30 | 2020-07-07 | 黑龙江省农业科学院经济作物研究所 | Enzyme method flax retting technology |
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| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
| CN101285211A (en) * | 2008-06-05 | 2008-10-15 | 黑龙江省科学院亚麻综合利用研究所 | Microorganism retting flax method for crudefiber crops |
| CN101575738A (en) * | 2009-06-15 | 2009-11-11 | 大连工业大学 | Method for degumming flax bast fiber microbe in solid state |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MX2011007504A (en) * | 2009-01-13 | 2011-12-16 | Ca Nat Research Council | Enzymatic preparation of plant fibers. |
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Patent Citations (3)
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|---|---|---|---|---|
| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
| CN101285211A (en) * | 2008-06-05 | 2008-10-15 | 黑龙江省科学院亚麻综合利用研究所 | Microorganism retting flax method for crudefiber crops |
| CN101575738A (en) * | 2009-06-15 | 2009-11-11 | 大连工业大学 | Method for degumming flax bast fiber microbe in solid state |
Non-Patent Citations (2)
| Title |
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| 亚麻雨露快速生物脱胶技术研究;陈浩等;《中国麻业科学》;20071231;第29卷(第6期);第339-343页 * |
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