CN102272319B - 用于提高核酸扩增反应效率的方法和组合物 - Google Patents

用于提高核酸扩增反应效率的方法和组合物 Download PDF

Info

Publication number
CN102272319B
CN102272319B CN201080004057.9A CN201080004057A CN102272319B CN 102272319 B CN102272319 B CN 102272319B CN 201080004057 A CN201080004057 A CN 201080004057A CN 102272319 B CN102272319 B CN 102272319B
Authority
CN
China
Prior art keywords
seq
variable region
polymerase
dna
reaction mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080004057.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN102272319A (zh
Inventor
龚晓松
约翰·苏利文
付荣典
王岩
埃文·H·伯西
程曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CN102272319A publication Critical patent/CN102272319A/zh
Application granted granted Critical
Publication of CN102272319B publication Critical patent/CN102272319B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201080004057.9A 2009-01-08 2010-01-07 用于提高核酸扩增反应效率的方法和组合物 Active CN102272319B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14335009P 2009-01-08 2009-01-08
US61/143,350 2009-01-08
PCT/US2010/020371 WO2010080910A1 (en) 2009-01-08 2010-01-07 Methods and compositions for improving efficiency of nucleic acids amplification reactions

Publications (2)

Publication Number Publication Date
CN102272319A CN102272319A (zh) 2011-12-07
CN102272319B true CN102272319B (zh) 2014-08-27

Family

ID=42316809

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080004057.9A Active CN102272319B (zh) 2009-01-08 2010-01-07 用于提高核酸扩增反应效率的方法和组合物

Country Status (7)

Country Link
US (3) US8859205B2 (https=)
EP (2) EP2382321A4 (https=)
JP (1) JP5818688B2 (https=)
CN (1) CN102272319B (https=)
CA (1) CA2749093C (https=)
SG (2) SG172925A1 (https=)
WO (1) WO2010080910A1 (https=)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
JP5818688B2 (ja) * 2009-01-08 2015-11-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 核酸増幅反応の効率を改善するための方法および組成物
CN103703141B (zh) * 2011-04-08 2016-08-17 伯乐实验室公司 具有降低的非特异性活性的pcr反应混合物
JP6060447B2 (ja) * 2011-04-08 2017-01-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 非特異的活性が低下したＳｓｏ７ポリメラーゼコンジュゲート
US8470573B2 (en) 2011-06-21 2013-06-25 Bio-Rad Laboratories, Inc. Hybrid polymerases having the ability to produce long amplicons
CN103045554B (zh) * 2012-11-29 2014-10-29 成都福际生物技术有限公司 一种制备热启动Taq酶的方法
JP2016512696A (ja) * 2013-03-15 2016-05-09 アーノルド, ライル, ジェイ.ARNOLD, Lyle, J. アセンブラ配列を利用する断片化された標的核酸の増幅方法
CN105452451B (zh) 2013-12-06 2020-06-05 生物辐射实验室股份有限公司 融合聚合酶
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
EP3778891A1 (en) * 2014-08-27 2021-02-17 New England Biolabs, Inc. Synthon formation
WO2017121836A1 (en) 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
WO2017121843A1 (en) * 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Antibodies that bind thermophilic dna polymerases
EP3645710A1 (en) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
CN109988817B (zh) * 2017-12-30 2020-12-04 安诺优达基因科技(北京)有限公司 一种随机打断dna的方法
WO2020264067A1 (en) 2019-06-25 2020-12-30 Bio-Rad Laboratories, Inc Compositions and methods for enhancing reverse transcriptase activity and/or reducing the inhibition of reverse transcriptase
US12173335B2 (en) 2020-01-06 2024-12-24 Pairwise Plants Services, Inc. Recruitment of DNA polymerase for templated editing
JP2021153393A (ja) * 2020-03-25 2021-10-07 東洋紡株式会社 核酸増幅反応用組成物
US20250145989A1 (en) * 2022-01-26 2025-05-08 Seegene, Inc. Method for detecting target nucleic acids in sample
WO2025116499A1 (en) * 2023-11-27 2025-06-05 Seegene, Inc. Method for analyzing activity of nucleic acid polymerase using self-dimer primer

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0821058A2 (en) * 1996-07-25 1998-01-28 The Institute Of Physical & Chemical Research Method for enhancing enzyme activity at elevated temperature
US6025545A (en) * 1990-01-22 2000-02-15 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
WO2004011605A2 (en) * 2002-07-25 2004-02-05 Mj Bioworks Incorporated Hybrid protein methods and compositions
US20040219558A1 (en) * 2002-07-25 2004-11-04 Mj Bioworks Incorporated Novel compositions with polymerase activity

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU22545A1 (es) * 1994-11-18 1999-03-31 Centro Inmunologia Molecular Obtención de un anticuerpo quimérico y humanizado contra el receptor del factor de crecimiento epidérmico para uso diagnóstico y terapéutico
US4545985A (en) 1984-01-26 1985-10-08 The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services Pseudomonas exotoxin conjugate immunotoxins
US4751180A (en) 1985-03-28 1988-06-14 Chiron Corporation Expression using fused genes providing for protein product
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5679543A (en) 1985-08-29 1997-10-21 Genencor International, Inc. DNA sequences, vectors and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
US4935233A (en) 1985-12-02 1990-06-19 G. D. Searle And Company Covalently linked polypeptide cell modulators
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
DK0528767T3 (da) * 1991-08-21 2000-04-17 Novartis Ag antistofderivater
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5914110A (en) * 1993-09-07 1999-06-22 Smithkline Beecham Corporation Recombinant IL4 antibodies useful in treatment of IL4 mediated disorders
DE69531487T2 (de) * 1994-01-31 2004-06-17 The Regents Of The University Of California, Oakland Methode zur eliminierung von sequenzierartefakten
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6015668A (en) 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
WO1997009451A1 (en) 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP3206894B2 (ja) * 1996-07-25 2001-09-10 理化学研究所 酵素を熱活性化する方法
US6972323B1 (en) * 1997-04-01 2005-12-06 Sankyo Company, Limited Anti-Fas antibodies
US5882904A (en) 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
AU3534199A (en) * 1998-04-23 1999-11-08 Takara Shuzo Co., Ltd. Method For Synthesizing DNA
EP1111044A4 (en) * 1998-09-08 2003-06-04 Takara Shuzo Co METHOD OF DNA SYNTHESIS
US20040031072A1 (en) * 1999-05-06 2004-02-12 La Rosa Thomas J. Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement
JP3589912B2 (ja) * 1999-09-03 2004-11-17 泰久 福井 ホスファチジルイノシトール−３，４−二リン酸を認識するモノクローナル抗体
IT1307309B1 (it) * 1999-12-30 2001-10-30 Enea Ente Nuove Tec Peptidi stabilizzanti, polipeptidi ed anticorpi che li comprendono.
US20040038339A1 (en) * 2000-03-24 2004-02-26 Peter Kufer Multifunctional polypeptides comprising a binding site to an epitope of the nkg2d receptor complex
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
US7408041B2 (en) * 2000-12-08 2008-08-05 Alexion Pharmaceuticals, Inc. Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof
US20040131607A1 (en) * 2001-06-08 2004-07-08 Carroll Michael C. Methods and compositions for inhibiting immunoglobulin-mediated reperfusion injury
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US7365167B2 (en) * 2001-11-26 2008-04-29 Cell Matrix, Inc. Humanized collagen antibodies and related methods
EP1456394B2 (en) 2001-11-28 2011-04-27 Bio-Rad Laboratories, Inc. Methods of using improved polymerases
DE60235491D1 (de) 2001-11-28 2010-04-08 Bio Rad Laboratories Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
US7666645B2 (en) 2002-10-23 2010-02-23 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugate proteins
WO2004038007A2 (en) * 2002-10-25 2004-05-06 Stratagene Dna polymerases with reduced base analog detection activity
US8283148B2 (en) * 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
AU2004225520A1 (en) 2003-03-25 2004-10-14 Stratagene DNA polymerase fusions and uses thereof
US20040214194A1 (en) 2003-04-17 2004-10-28 Mj Bioworks, Inc. Methods of making hybrid proteins
US20050025763A1 (en) * 2003-05-08 2005-02-03 Protein Design Laboratories, Inc. Therapeutic use of anti-CS1 antibodies
US7445898B2 (en) 2004-04-01 2008-11-04 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
US20060005174A1 (en) 2004-07-01 2006-01-05 International Business Machines Corporation Defining hierarchical structures with markup languages and reflection
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
CA2615151A1 (en) * 2005-07-15 2007-01-25 Stratagene California Dna binding protein-polymerase chimeras
ES2543341T3 (es) * 2005-09-13 2015-08-18 National Research Council Of Canada Métodos y composiciones para modular la actividad de células tumorales
EP1966243B1 (en) * 2005-12-23 2012-02-15 Eli Lilly And Company Tgf-beta binding antibodies
US9085762B2 (en) * 2006-12-29 2015-07-21 Agilent Technologies, Inc. Compositions and methods using split polymerases
MX2009008430A (es) * 2007-02-09 2009-10-28 Genentech Inc Anticuerpos anti-robo4 y sus usos.
WO2009140497A1 (en) 2008-05-16 2009-11-19 New England Biolabs, Inc. Enzyme reagents for amplification of polynucleotides in the presence of inhibitors
JP5818688B2 (ja) * 2009-01-08 2015-11-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 核酸増幅反応の効率を改善するための方法および組成物

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025545A (en) * 1990-01-22 2000-02-15 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
EP0821058A2 (en) * 1996-07-25 1998-01-28 The Institute Of Physical & Chemical Research Method for enhancing enzyme activity at elevated temperature
WO2004011605A2 (en) * 2002-07-25 2004-02-05 Mj Bioworks Incorporated Hybrid protein methods and compositions
US20040219558A1 (en) * 2002-07-25 2004-11-04 Mj Bioworks Incorporated Novel compositions with polymerase activity

Also Published As

Publication number Publication date
US9994887B2 (en) 2018-06-12
SG10201500705YA (en) 2015-04-29
US8859205B2 (en) 2014-10-14
WO2010080910A1 (en) 2010-07-15
EP2382321A4 (en) 2012-12-19
EP2894226B1 (en) 2021-04-14
SG172925A1 (en) 2011-08-29
CN102272319A (zh) 2011-12-07
CA2749093C (en) 2019-03-26
JP5818688B2 (ja) 2015-11-18
EP2382321A1 (en) 2011-11-02
CA2749093A1 (en) 2010-07-15
EP2894226A1 (en) 2015-07-15
US10787694B2 (en) 2020-09-29
US20110104760A1 (en) 2011-05-05
US20140377767A1 (en) 2014-12-25
US20180258455A1 (en) 2018-09-13
JP2012514473A (ja) 2012-06-28

Similar Documents

Publication Publication Date Title
CN102272319B (zh) 用于提高核酸扩增反应效率的方法和组合物
US9556423B2 (en) PCR reaction mixtures with decreased non-specific activity
EP2723880B1 (en) Hybrid polymerases having the ability to produce long amplicons
US9322002B2 (en) Sso7-polymerase conjugates with decreased non-specific activity
US10351831B2 (en) Fusion polymerases
EP3172332B1 (en) Buffers for use with polymerases

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant