CA2749093C - Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions - Google Patents
Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions Download PDFInfo
- Publication number
- CA2749093C CA2749093C CA2749093A CA2749093A CA2749093C CA 2749093 C CA2749093 C CA 2749093C CA 2749093 A CA2749093 A CA 2749093A CA 2749093 A CA2749093 A CA 2749093A CA 2749093 C CA2749093 C CA 2749093C
- Authority
- CA
- Canada
- Prior art keywords
- polymerase
- seq
- hybrid
- dna
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14335009P | 2009-01-08 | 2009-01-08 | |
| US61/143,350 | 2009-01-08 | ||
| PCT/US2010/020371 WO2010080910A1 (en) | 2009-01-08 | 2010-01-07 | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2749093A1 CA2749093A1 (en) | 2010-07-15 |
| CA2749093C true CA2749093C (en) | 2019-03-26 |
Family
ID=42316809
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2749093A Active CA2749093C (en) | 2009-01-08 | 2010-01-07 | Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US8859205B2 (https=) |
| EP (2) | EP2382321A4 (https=) |
| JP (1) | JP5818688B2 (https=) |
| CN (1) | CN102272319B (https=) |
| CA (1) | CA2749093C (https=) |
| SG (2) | SG10201500705YA (https=) |
| WO (1) | WO2010080910A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9181551B2 (en) | 2002-02-20 | 2015-11-10 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
| US9657294B2 (en) | 2002-02-20 | 2017-05-23 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
| EP2382321A4 (en) * | 2009-01-08 | 2012-12-19 | Bio Rad Laboratories | METHODS AND COMPOSITIONS FOR ENHANCING THE EFFICACY OF NUCLEIC ACID AMPLIFICATION REACTIONS |
| SG193990A1 (en) * | 2011-04-08 | 2013-11-29 | Bio Rad Laboratories | Pcr reaction mixtures with decreased non-specific activity |
| JP6060447B2 (ja) * | 2011-04-08 | 2017-01-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 非特異的活性が低下したＳｓｏ７ポリメラーゼコンジュゲート |
| US8470573B2 (en) * | 2011-06-21 | 2013-06-25 | Bio-Rad Laboratories, Inc. | Hybrid polymerases having the ability to produce long amplicons |
| CN103045554B (zh) * | 2012-11-29 | 2014-10-29 | 成都福际生物技术有限公司 | 一种制备热启动Taq酶的方法 |
| EP2971134B1 (en) * | 2013-03-15 | 2023-10-25 | Aegea Biotechnologies, Inc. | Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence |
| EP2986719B1 (en) | 2013-12-06 | 2020-07-29 | Bio-Rad Laboratories, Inc. | Fusion polymerases |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| JP6594955B2 (ja) * | 2014-08-27 | 2019-10-23 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | シントン形成 |
| US10273530B2 (en) | 2016-01-15 | 2019-04-30 | Thermo Fisher Scientific Baltics Uab | Antibodies that bind thermophilic DNA polymerases |
| US10626383B2 (en) | 2016-01-15 | 2020-04-21 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| KR102380264B1 (ko) * | 2017-08-31 | 2022-03-29 | 주식회사 씨젠 | 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가 |
| CN112375807B (zh) * | 2017-12-30 | 2023-02-21 | 浙江安诺优达生物科技有限公司 | 一种随机打断dna的方法 |
| WO2020264067A1 (en) | 2019-06-25 | 2020-12-30 | Bio-Rad Laboratories, Inc | Compositions and methods for enhancing reverse transcriptase activity and/or reducing the inhibition of reverse transcriptase |
| WO2021141970A1 (en) * | 2020-01-06 | 2021-07-15 | Pairwise Plants Services, Inc. | Recruitment of dna polymerase for templated editing |
| JP2021153393A (ja) * | 2020-03-25 | 2021-10-07 | 東洋紡株式会社 | 核酸増幅反応用組成物 |
| KR20240141247A (ko) * | 2022-01-26 | 2024-09-26 | 주식회사 씨젠 | 샘플 내 타겟 핵산을 검출하는 방법 |
| WO2025116499A1 (en) * | 2023-11-27 | 2025-06-05 | Seegene, Inc. | Method for analyzing activity of nucleic acid polymerase using self-dimer primer |
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| CU22545A1 (es) * | 1994-11-18 | 1999-03-31 | Centro Inmunologia Molecular | Obtención de un anticuerpo quimérico y humanizado contra el receptor del factor de crecimiento epidérmico para uso diagnóstico y terapéutico |
| US4545985A (en) | 1984-01-26 | 1985-10-08 | The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services | Pseudomonas exotoxin conjugate immunotoxins |
| US4751180A (en) | 1985-03-28 | 1988-06-14 | Chiron Corporation | Expression using fused genes providing for protein product |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5679543A (en) | 1985-08-29 | 1997-10-21 | Genencor International, Inc. | DNA sequences, vectors and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi |
| US4935233A (en) | 1985-12-02 | 1990-06-19 | G. D. Searle And Company | Covalently linked polypeptide cell modulators |
| US6025545A (en) | 1990-01-22 | 2000-02-15 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| DE69230545T2 (de) * | 1991-08-21 | 2000-07-06 | Novartis Ag, Basel | Antikörperderivate |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5914110A (en) * | 1993-09-07 | 1999-06-22 | Smithkline Beecham Corporation | Recombinant IL4 antibodies useful in treatment of IL4 mediated disorders |
| DE69531487T2 (de) * | 1994-01-31 | 2004-06-17 | The Regents Of The University Of California, Oakland | Methode zur eliminierung von sequenzierartefakten |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US6015668A (en) | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
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| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
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| EP1033411B1 (en) * | 1996-06-04 | 2006-02-22 | University of Utah Research Foundation | Fluorescent donor-acceptor pair |
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-
2010
- 2010-01-07 EP EP10729512A patent/EP2382321A4/en not_active Withdrawn
- 2010-01-07 EP EP15155113.2A patent/EP2894226B1/en active Active
- 2010-01-07 US US12/683,950 patent/US8859205B2/en active Active
- 2010-01-07 WO PCT/US2010/020371 patent/WO2010080910A1/en not_active Ceased
- 2010-01-07 CA CA2749093A patent/CA2749093C/en active Active
- 2010-01-07 SG SG10201500705YA patent/SG10201500705YA/en unknown
- 2010-01-07 CN CN201080004057.9A patent/CN102272319B/zh active Active
- 2010-01-07 JP JP2011545427A patent/JP5818688B2/ja active Active
- 2010-01-07 SG SG2011050093A patent/SG172925A1/en unknown
-
2014
- 2014-09-10 US US14/482,756 patent/US9994887B2/en active Active
-
2018
- 2018-05-11 US US15/977,254 patent/US10787694B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2012514473A (ja) | 2012-06-28 |
| EP2894226B1 (en) | 2021-04-14 |
| US20140377767A1 (en) | 2014-12-25 |
| SG172925A1 (en) | 2011-08-29 |
| US10787694B2 (en) | 2020-09-29 |
| WO2010080910A1 (en) | 2010-07-15 |
| CN102272319B (zh) | 2014-08-27 |
| US8859205B2 (en) | 2014-10-14 |
| CN102272319A (zh) | 2011-12-07 |
| CA2749093A1 (en) | 2010-07-15 |
| EP2382321A4 (en) | 2012-12-19 |
| EP2894226A1 (en) | 2015-07-15 |
| US20180258455A1 (en) | 2018-09-13 |
| US20110104760A1 (en) | 2011-05-05 |
| SG10201500705YA (en) | 2015-04-29 |
| EP2382321A1 (en) | 2011-11-02 |
| US9994887B2 (en) | 2018-06-12 |
| JP5818688B2 (ja) | 2015-11-18 |
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