CN102272146A - 用带负电的聚合物沉淀生物分子 - Google Patents

用带负电的聚合物沉淀生物分子 Download PDF

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Publication number
CN102272146A
CN102272146A CN2010800048316A CN201080004831A CN102272146A CN 102272146 A CN102272146 A CN 102272146A CN 2010800048316 A CN2010800048316 A CN 2010800048316A CN 201080004831 A CN201080004831 A CN 201080004831A CN 102272146 A CN102272146 A CN 102272146A
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CN
China
Prior art keywords
polymer
aqueous
antibody
precipitate
paa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2010800048316A
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English (en)
Chinese (zh)
Inventor
J·范阿尔斯廷
J·沙纳加尔
R·霍尔思
K·莱基
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
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GE Healthcare Bio Sciences AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of CN102272146A publication Critical patent/CN102272146A/zh
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39591—Stabilisation, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN2010800048316A 2009-01-13 2010-01-11 用带负电的聚合物沉淀生物分子 Pending CN102272146A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0900032 2009-01-13
SE0900032-4 2009-01-13
PCT/SE2010/050017 WO2010082894A1 (en) 2009-01-13 2010-01-11 Precipitation of biomolecules with negatively charged polymers

Publications (1)

Publication Number Publication Date
CN102272146A true CN102272146A (zh) 2011-12-07

Family

ID=42339999

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010800048316A Pending CN102272146A (zh) 2009-01-13 2010-01-11 用带负电的聚合物沉淀生物分子

Country Status (7)

Country Link
US (1) US20120022239A1 (https=)
EP (1) EP2376523A4 (https=)
JP (1) JP2012515161A (https=)
CN (1) CN102272146A (https=)
AU (1) AU2010205007A1 (https=)
CA (1) CA2748809A1 (https=)
WO (1) WO2010082894A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114574457A (zh) * 2022-01-25 2022-06-03 华东理工大学 一种快速分离纯化蛋白质的方法

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US20120177610A1 (en) 2007-09-19 2012-07-12 Kieu Hoang Manufacturing and Purification Processes of Complex Protein found in Fraction IV to make a separated Apo, Transferrin , and Alpha 1 Anti strepsin (A1AT) or A combined Transferrin / Apo/Human Albumin/A1AT and all new found proteins
WO2009151514A1 (en) 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
CN102257122B (zh) 2008-12-16 2015-07-29 Emd密理博公司 搅拌槽反应器及方法
CN107312062B (zh) 2010-05-17 2021-03-16 Emd密理博公司 用于纯化生物分子的刺激响应性聚合物
JP5950477B2 (ja) * 2011-08-10 2016-07-13 アドシア 少なくとも１種の基礎インスリンの注射溶液
WO2013039449A1 (en) * 2011-09-16 2013-03-21 Ge Healthcare Bio-Sciences Ab Plasma protein fractionation by sequential polyacid precipitation
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
AR089231A1 (es) 2011-12-15 2014-08-06 Amgen Inc Metodo de floculacion
TW201335181A (zh) * 2012-01-31 2013-09-01 Kieu Hoang ５５種新發現的蛋白質之序列及其應用
CN103293299B (zh) * 2012-02-24 2016-06-08 珀金埃尔默医学诊断产品（上海）有限公司 拓宽双抗体夹心免疫检测浓度范围的方法及其试剂盒
NO2994485T3 (https=) 2013-05-06 2018-06-09
WO2015126523A1 (en) 2014-02-21 2015-08-27 Clontech Laboratories, Inc. Spin columns comprising poly(acid) membrane separation matrices, and methods of making and using the same
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
WO2020036869A1 (en) 2018-08-16 2020-02-20 Emd Millipore Corporation Closed bioprocessing device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080193981A1 (en) * 2007-01-22 2008-08-14 Fahrner Robert L Polyelectrolyte precipitation and purification of proteins

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE348942B (https=) * 1970-06-02 1972-09-18 Statens Bakteriologiska Labor
US5976382A (en) * 1996-07-10 1999-11-02 Ligochem, Inc. Removal of proteins from aqueuos media by precipitation
DE102005003500A1 (de) * 2005-01-25 2006-07-27 Saint-Gobain Industriekeramik Rödental GmbH Brennguttragevorrichtung mit elastischer Stützenfixierung
US8362217B2 (en) * 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008100578A2 (en) * 2007-02-14 2008-08-21 Amgen Inc. Method of isolating antibodies by precipitation
JP2010530414A (ja) * 2007-06-19 2010-09-09 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー ポリマー多相システムを用いた分離方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080193981A1 (en) * 2007-01-22 2008-08-14 Fahrner Robert L Polyelectrolyte precipitation and purification of proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MCDONALD P. ET AL.: "Selective antibody precipitation using polyelectrolytes: a novel approach to the purifiation of monoclonal antibodies.", 《BIOTECHNOLOGY AND BIOENGINEERING》 *
无: "CAPTO MMC", 《GE HEALTHCARE DATA FILE 11-0035-45 AA MULTIMODAL MEDIA》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114574457A (zh) * 2022-01-25 2022-06-03 华东理工大学 一种快速分离纯化蛋白质的方法

Also Published As

Publication number Publication date
EP2376523A1 (en) 2011-10-19
WO2010082894A1 (en) 2010-07-22
JP2012515161A (ja) 2012-07-05
US20120022239A1 (en) 2012-01-26
CA2748809A1 (en) 2010-07-22
AU2010205007A1 (en) 2011-07-07
EP2376523A4 (en) 2012-11-28

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SE01 Entry into force of request for substantive examination
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WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20111207