CA2748809A1 - Precipitation of biomolecules with negatively charged polymers - Google Patents
Precipitation of biomolecules with negatively charged polymers Download PDFInfo
- Publication number
- CA2748809A1 CA2748809A1 CA2748809A CA2748809A CA2748809A1 CA 2748809 A1 CA2748809 A1 CA 2748809A1 CA 2748809 A CA2748809 A CA 2748809A CA 2748809 A CA2748809 A CA 2748809A CA 2748809 A1 CA2748809 A1 CA 2748809A1
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- Prior art keywords
- polymer
- precipitate
- biomolecule
- antibody
- paa
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
- A61K39/39591—Stabilisation, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/32—Extraction; Separation; Purification by precipitation as complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3847—Multimodal interactions
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0900032 | 2009-01-13 | ||
| SE0900032-4 | 2009-01-13 | ||
| PCT/SE2010/050017 WO2010082894A1 (en) | 2009-01-13 | 2010-01-11 | Precipitation of biomolecules with negatively charged polymers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2748809A1 true CA2748809A1 (en) | 2010-07-22 |
Family
ID=42339999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2748809A Abandoned CA2748809A1 (en) | 2009-01-13 | 2010-01-11 | Precipitation of biomolecules with negatively charged polymers |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20120022239A1 (https=) |
| EP (1) | EP2376523A4 (https=) |
| JP (1) | JP2012515161A (https=) |
| CN (1) | CN102272146A (https=) |
| AU (1) | AU2010205007A1 (https=) |
| CA (1) | CA2748809A1 (https=) |
| WO (1) | WO2010082894A1 (https=) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2902799B1 (fr) | 2006-06-27 | 2012-10-26 | Millipore Corp | Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide |
| US8569464B2 (en) | 2006-12-21 | 2013-10-29 | Emd Millipore Corporation | Purification of proteins |
| WO2008079280A1 (en) | 2006-12-21 | 2008-07-03 | Millipore Corporation | Purification of proteins |
| US8362217B2 (en) | 2006-12-21 | 2013-01-29 | Emd Millipore Corporation | Purification of proteins |
| US20120177610A1 (en) | 2007-09-19 | 2012-07-12 | Kieu Hoang | Manufacturing and Purification Processes of Complex Protein found in Fraction IV to make a separated Apo, Transferrin , and Alpha 1 Anti strepsin (A1AT) or A combined Transferrin / Apo/Human Albumin/A1AT and all new found proteins |
| WO2009151514A1 (en) | 2008-06-11 | 2009-12-17 | Millipore Corporation | Stirred tank bioreactor |
| CN102257122B (zh) | 2008-12-16 | 2015-07-29 | Emd密理博公司 | 搅拌槽反应器及方法 |
| CN107312062B (zh) | 2010-05-17 | 2021-03-16 | Emd密理博公司 | 用于纯化生物分子的刺激响应性聚合物 |
| JP5950477B2 (ja) * | 2011-08-10 | 2016-07-13 | アドシア | 少なくとも１種の基礎インスリンの注射溶液 |
| WO2013039449A1 (en) * | 2011-09-16 | 2013-03-21 | Ge Healthcare Bio-Sciences Ab | Plasma protein fractionation by sequential polyacid precipitation |
| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| AR089231A1 (es) | 2011-12-15 | 2014-08-06 | Amgen Inc | Metodo de floculacion |
| TW201335181A (zh) * | 2012-01-31 | 2013-09-01 | Kieu Hoang | ５５種新發現的蛋白質之序列及其應用 |
| CN103293299B (zh) * | 2012-02-24 | 2016-06-08 | 珀金埃尔默医学诊断产品（上海）有限公司 | 拓宽双抗体夹心免疫检测浓度范围的方法及其试剂盒 |
| NO2994485T3 (https=) | 2013-05-06 | 2018-06-09 | ||
| WO2015126523A1 (en) | 2014-02-21 | 2015-08-27 | Clontech Laboratories, Inc. | Spin columns comprising poly(acid) membrane separation matrices, and methods of making and using the same |
| GB2539420B (en) * | 2015-06-16 | 2021-01-13 | Cytiva Sweden Ab | Determination of chromatography conditions |
| EP3546475A1 (en) | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| WO2020036869A1 (en) | 2018-08-16 | 2020-02-20 | Emd Millipore Corporation | Closed bioprocessing device |
| CN114574457B (zh) * | 2022-01-25 | 2024-07-23 | 华东理工大学 | 一种快速分离纯化蛋白质的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE348942B (https=) * | 1970-06-02 | 1972-09-18 | Statens Bakteriologiska Labor | |
| US5976382A (en) * | 1996-07-10 | 1999-11-02 | Ligochem, Inc. | Removal of proteins from aqueuos media by precipitation |
| DE102005003500A1 (de) * | 2005-01-25 | 2006-07-27 | Saint-Gobain Industriekeramik Rödental GmbH | Brennguttragevorrichtung mit elastischer Stützenfixierung |
| US8362217B2 (en) * | 2006-12-21 | 2013-01-29 | Emd Millipore Corporation | Purification of proteins |
| NZ577933A (en) * | 2007-01-22 | 2011-12-22 | Genentech Inc | Polyelectrolyte precipitation and purification of antibodies |
| WO2008100578A2 (en) * | 2007-02-14 | 2008-08-21 | Amgen Inc. | Method of isolating antibodies by precipitation |
| JP2010530414A (ja) * | 2007-06-19 | 2010-09-09 | ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー | ポリマー多相システムを用いた分離方法 |
-
2010
- 2010-01-11 US US13/144,100 patent/US20120022239A1/en not_active Abandoned
- 2010-01-11 AU AU2010205007A patent/AU2010205007A1/en not_active Abandoned
- 2010-01-11 JP JP2011545324A patent/JP2012515161A/ja active Pending
- 2010-01-11 CA CA2748809A patent/CA2748809A1/en not_active Abandoned
- 2010-01-11 CN CN2010800048316A patent/CN102272146A/zh active Pending
- 2010-01-11 WO PCT/SE2010/050017 patent/WO2010082894A1/en not_active Ceased
- 2010-01-11 EP EP10731439A patent/EP2376523A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP2376523A1 (en) | 2011-10-19 |
| WO2010082894A1 (en) | 2010-07-22 |
| CN102272146A (zh) | 2011-12-07 |
| JP2012515161A (ja) | 2012-07-05 |
| US20120022239A1 (en) | 2012-01-26 |
| AU2010205007A1 (en) | 2011-07-07 |
| EP2376523A4 (en) | 2012-11-28 |
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