CN102258036B - Bactericide and application of bactericide in prevention and control of rice sheath blight and strawberry anthracnose - Google Patents

Bactericide and application of bactericide in prevention and control of rice sheath blight and strawberry anthracnose Download PDF

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CN102258036B
CN102258036B CN 201110234198 CN201110234198A CN102258036B CN 102258036 B CN102258036 B CN 102258036B CN 201110234198 CN201110234198 CN 201110234198 CN 201110234198 A CN201110234198 A CN 201110234198A CN 102258036 B CN102258036 B CN 102258036B
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bactericide
thifluzamide
control
epoxiconazole
sheath blight
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CN102258036A (en
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吉沐祥
庄义庆
姚克兵
王健
束兆林
缪康
赵来成
吴祥
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Jiangsu Lvdun Plant Protection Agrochemlcal Experimental Co Ltd
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Jiangsu Lvdun Plant Protection Agrochemlcal Experimental Co Ltd
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Abstract

The invention discloses a bactericide and application of the bactericide to the prevention and control of rice sheath blight and strawberry anthracnose, and belongs to pesticides. The bactericide comprises thifluzamide and epoxiconazole in a mass ratio ranging from 1:50 to 50:1, wherein the thifluzamide and the epoxiconazole account for 1 to 60 percent of the total mass. The invention also provides application of the bactericide to the prevention and control of the rice sheath blight and the strawberry anthracnose. Due to the mixing of the thifluzamide and the epoxiconazole, the bactericide has the obvious synergic effect, the using dose is reduced, a bactericidal spectrum is enlarged, and the characteristics of the bactericide are complementary to each other, and the bactericide can prevent and control diseases of various crops and is convenient and practical. In addition, the mixed thifluzamide and the epoxiconazole are hardly toxic, do not have resides and have a great significance for the resistance and comprehensive treatment of the diseases.

Description

A kind of bactericide and the application on control strawberry anthracnose thereof
Technical field
The present invention designs a kind of agricultural chemicals, relates in particular to a kind of bactericide of preventing and treating rice sheath blight disease and plant anthracnose.
Background technology
Reform along with cropping system, China existing high-quality, high-yield rice kind than the late-maturing type of establishing in large scale, and follow promoting the use of of climate warming, light cultivation technology, harm takes place and totally is the situation that rises significantly in rice sheath blight disease over past ten years, in south China, the south of the River, the Yangtze river basin and Yangze river and Huai river rice district lay particular stress on generation, disease serious threat rice safety is produced.
Strawberry anthracnose is that one of comparatively general disease takes place in southern strawberry producing region.Strawberry anthracnose mainly occurs in nursery stage (stolon is taken out the living phase) and field planting initial stage, and fruiting period is rare.Its main harm stolon, petiole, blade, stipule, petal, calyx and fruit.
Thifluzamide also claims the thiophene flutolanil, and English common name Trifluzamide belongs to the thiazole amide bactericide, has strong interior conductivity and the long lasting effect of inhaling.Thifluzamide is the succinate dehydrogenase inhibitor, because fluorine-containing, its competitiveness in biochemical pathway is very strong, in case combine with regard to difficult recovery with substrate or enzyme.Thifluzamide has special efficacy to the fungus-caused disease of Basidiomycetes such as banded sclerotial blight, damping off etc., and pathomycetes such as Rhizoctonia, Puccinia, Ustilago, Tilletia, corticium, nuclear cavity Pseudomonas are also all had good preventive effect.
Fluorine ring azoles, English common name Epoxiconazole, it is the fluorine-containing wide-spectrum bactericide of a kind of novel important triazole type, activity is high, toxicity is low good with Environmental compatibility.Ten multiple diseases such as a series of cereal crops such as banded sclerotial blight, damping off, powdery mildew, ring spot are had good preventive and therapeutic effect, and can prevent and treat diseases such as sugar beet, peanut, rape, tomato, watermelon, strawberry and fruit tree.Not only have good protection, treat and root out activity, and inhale and preferable residual activity in having.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of bactericide that combines as main active by thifluzamide and fluorine ring azoles, rice sheath blight disease and strawberry anthracnose are had better control efficiency.
Technical scheme: for a kind of bactericide is provided, the present invention includes thifluzamide and fluorine ring azoles, the mass ratio of described thifluzamide and fluorine ring azoles is 1: 50～50: 1, and described thifluzamide and fluorine ring azoles account for 1～60% of gross mass.
As further optimization of the present invention, the mass ratio of described thifluzamide and fluorine ring azoles is 2: 5～5: 2, and described thifluzamide and fluorine ring azoles account for 15～60% of gross mass.
Described thifluzamide and fluorine ring azoles are the main active ingredient of this bactericide, and all the other compositions comprise surfactant, stabilizing agent, antifreezing agent, thickener, disintegrant, plant growth regulator, carrier, and the compound with weeding, desinsection or bactericidal property.The formulation of described bactericide is suspending agent, aqueous emulsion, wetting powder or water dispersible granules.
Further optimize as the present invention, described surfactant is wetting agent or dispersant or emulsifier, and described surfactant comprises ionic and nonionic.Wherein, described wetting agent is natural products wetting agent and cationic wetting agent, comprises Tea Saponin, silkworm excrement, bay alcohol radical sodium sulphate, lauryl sodium sulfate, draws back in powder etc. one or more; Dispersant is industrial by-products and anionic dispersing agent, comprises in lignosulfite, lignin and derivative sulfonate thereof, the bitter edible plant and alkyl bitter edible plant methyl alcohol condensate sulfonates, the alkylsulfonate one or more; Emulsifier comprises one or more of nonionic emulsifier such as polyoxyethylene fatty acid ester, polyoxyethylene aliphatic alcohol ether, polyoxyethylene fat glue, ethoxylated castor oil and anionic emulsifier.
As improvement of the present invention, described thickener comprises one or more of water-soluble high-molecular compound, water-soluble resin gum Arabic, xanthans, methylcellulose, carboxymethyl cellulose; Described antifreezing agent comprises one or more of ethylene glycol, propane diols, glycerine; Described defoamer comprises organic silicone, C 8-10Fatty alcohol, C 10-20Representative examples of saturated aliphatic carboxylic and ester class thereof, ester-ether type compound etc., one or more of organic silicone commonly used and ester-ether type defoamer.
Wherein, described carrier comprises solid carrier and liquid-carrier.Described solid carrier comprises one or more of natural sticking scholar, silicate, magnesium silicate, Magnesiumaluminumsilicate, alumina silicate, calcium carbonate, calcium sulphate, ammonium sulfate, hydrated silica, calcium silicates, alumina silicate, also comprises the attapulgite, bentonite, montmorillonite, the diatomite that are suitable for making pulvis.
Described liquid-carrier comprises water and organic solvent.Water can be used as solvent or thinner, and organic solvent also can be used as secondary solvent or stabilization additives, and described organic solvent comprises aromatic hydrocarbons, for example toluene, dimethylbenzene, benzene Fluhyzon and chlorinated aromatic hydrocarbons, chloro aliphatic hydrocarbon, aliphatic hydrocarbon.Particularly suitable be polar solvent, for example isopropyl alcohol of alcohols, butanols, ethylene glycol, cyclohexanol, ketone such as acetone, MEK, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO) and N-methyl adjoin pyrrolidone.
The present invention relates to a kind of bactericide in the application that prevents and treats on rice sheath blight disease and the strawberry anthracnose.Simultaneously, the invention still further relates to application on fungus-caused damping off, controlling powdery mildew by Basidiomycetes.In addition, the present invention is also to by Rhizoctonia, Puccinia, Ustilago, Tilletia, photovoltaicing leather bacteria section, nuclear cavity Pseudomonas.
In order to understand essence of the present invention better, will prepare bactericide as main active with thifluzamide and fluorine ring azoles below, prove that by virulence test it is preventing and treating the effect of rice sheath blight disease.
Prepare former medicine of 96% thifluzamide and 97% epoxiconazole raw drug, the former medicine of thifluzamide is mixed with 9 groups of bactericide mixture processed group with epoxiconazole raw drug by different proportionings, thifluzamide (A), fluorine ring azoles (B) are organized respectively in contrast.Pot-culture method is adopted in test.Select two leaves wholeheartedly, the potted plant rice seedling of growing way unanimity, spraying is handled the back and is dried naturally, 24h inoculates, and is spraying a banded sclerotial blight mycelia of the central clamping connection of the every basin rice seedling base portion piece of medicine, the bacterium piece does not contact with basin soil and water layer.Postvaccinal examination material moves in the humidistat to be cultivated, 26 ℃ of temperature, and humidity 80～90% is observed the rice growth situation every day, investigation prevention effect when blank is fully fallen ill behind the 5d.
Wherein, investigation method adopts sick leaf disease feelings is scaled co-toxicity coefficient, with the control efficiency of reflection bactericide.It is as follows to set up graded index earlier:
0 grade---do not fall ill;
1 grade---the morbidity of first leaf sheath;
3 grades---first blade and the morbidity of second leaf sheath;
5 grades---second blade and the morbidity of second leaf sheath;
7 grades---whole strain morbidity.
Wherein:
Disease index=(morbidity number * graded index at different levels) * 100/ (investigation seedling number * 7)
Control efficiency (%)=(contrast disease index-processing disease index) * 100/ contrast disease index
Calculate the control efficiency of each processed group with disease index, carry out the data analysis statistics, obtain regression line, EC with " DPS data handling system V3.11 " 50, correlation coefficient.EC that each that will measure according to Sun Yunpei (Y-P Sun) method handled 50Be converted into actual poison exponent (ATI),, obtain theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula according to the proportioning of mixture.
Poison exponent=standard medicament EC 50* 100/ reagent agent EC 50
Actual measurement (using with) toxicity index (ATI)=standard medicament EC 50* 100/ for examination (using with) medicament EC 50
The content (%) of toxicity index * B in mixture of content (the %)+B of toxicity index * A in mixture of theoretical (using with) toxicity index (TTI)=A
Co-toxicity coefficient (CTC)=actual measurement (using with) toxicity index (ATI) * 100/ theory (using with) toxicity index
If co-toxicity coefficient is greater than 120, showing has synergistic effect: if be starkly lower than 100, be indicated as antagonism; Between 100～120, be indicated as summation action.
Table 1 thifluzamide and fluorine ring azoles and different proportioning are to rice sheath blight disease toxicity test result
Figure BDA0000083627740000031
Figure BDA0000083627740000041
As can be seen from Table 1, thifluzamide and fluorine ring azoles are mixed, and in mass ratio is 2: 5～5: 2 scope, rice sheath blight disease are all shown as synergistic effect.Thifluzamide and fluorine ring azoles are mixed and have reasonability and feasibility.
More than preventing and treating the effect of rice sheath blight disease by the present invention of virulence test proof, below, prepare bactericide with thifluzamide and fluorine ring azoles as main active, prove that by virulence test it is in the effect of preventing and treating strawberry anthracnose.
Prepare former medicine of 96% thifluzamide and 97% epoxiconazole raw drug, the former medicine of thifluzamide is mixed with 9 groups of bactericide mixture processed group with epoxiconazole raw drug by different proportionings, thifluzamide (C), fluorine ring azoles (D) are organized respectively in contrast.With reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".With strawberry anthrax bacteria PDA medium culture, when treating that bacterium colony has just covered with culture dish, punch from the edge with the card punch of internal diameter 7mm, the mycelia piece that breaks into is as inoculum.Get the soup 5ml for preparing and quantitative 75ml sterilising medium respectively and mix, make 4 pastille flat boards, be mixed into contrast with equivalent sterile water and medium.The inversion of mycelia piece is connected to dull and stereotyped central authorities, and in 28 ℃ of incubators, cultivates.Behind the 7d, measure colony diameter, calculate and respectively handle the clean amount of growth of mycelia, mycelial growth inhibition rate with the right-angled intersection method.
Clean amount of growth (mm)=measurement colony diameter-7
Figure BDA0000083627740000042
The mycelial growth rate conversion is become probability value (y), and liquor strength (ug/ml) converts logarithm value (x) to, calculates concentration EC in virulence equation and the inhibition with method of least squares 50, according to the toxicity index and the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun medicament.
Table 2 thifluzamide and fluorine ring azoles and different proportioning are to strawberry anthracnose toxicity test result
Figure BDA0000083627740000043
Table 2 shows that thifluzamide and fluorine ring azoles have synergistic effect to strawberry anthracnose between proportioning 5: 2～2: 5, and especially between 5: 4～3: 5, synergistic effect is obvious, and co-toxicity coefficient is all more than 120.
Beneficial effect: the present invention has notable synergistic by after thifluzamide and fluorine ring azoles are mixed to sterilization, has reduced using dosage, enlarges fungicidal spectrum, and the medicament characteristic has obtained mutual supplement with each other's advantages, and a kind of medicament can be prevented and treated the various crop disease, and is convenient and practical; And little poison, noresidue were significant to the resistance and the comprehensive regulation of disease after thifluzamide and fluorine ring azoles were mixed.
Embodiment
Come the present invention is done further explanation with embodiment below, the percentage in following examples is mass percent if no special instructions.
Embodiment 1
Figure BDA0000083627740000052
By the prescription requirement, be medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined batching mix in the still, after ball mill or clipper pulverizing just, porphyrize in the sand milling still is made suspending agent, through after the assay was approved, measuring packing is finished product.
Embodiment 2
By the prescription requirement, be medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined batching mix in the still, after ball mill or clipper pulverizing just, porphyrize in the sand milling still is made suspending agent, through after the assay was approved, measuring packing is finished product.
Embodiment 3
By the prescription requirement, be medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined batching mix in the still, after ball mill or clipper pulverizing just, porphyrize in the sand milling still is made suspending agent, through after the assay was approved, measuring packing is finished product.
Embodiment 4
Figure BDA0000083627740000063
By the prescription requirement, mix after after Roughpulverizer pulverizes, enter airslide disintegrating mill and fully pulverize mixing, through after the assay was approved, be packaged to be automatically wetting powder.
Embodiment 5
Figure BDA0000083627740000071
By the prescription requirement, mix after after Roughpulverizer pulverizes, enter airslide disintegrating mill and fully pulverize mixing, through after the assay was approved, be packaged to be automatically wetting powder.
Embodiment 6
Figure BDA0000083627740000072
By the prescription requirement, former medicine, dispersant, wetting agent, disintegrant, solid carrier etc. are mixed after comminution by gas stream becomes wetting powder, add suitable quantity of water then and carry out granulation and obtain water dispersible granules.
Embodiment 7
Figure BDA0000083627740000073
By the prescription requirement, former medicine, dispersant, wetting agent, disintegrant, solid carrier etc. are mixed after comminution by gas stream becomes wetting powder, add suitable quantity of water then and carry out granulation and obtain water dispersible granules.
Embodiment 8
By the prescription requirement, be medium with water, auxiliary agents such as former medicine, emulsifier, dispersant are joined in the batching still, mix through high-speed shearing machine and make aqueous emulsion, through after the assay was approved, measure packing and promptly obtain finished product.
Embodiment 9
Figure BDA0000083627740000082
By the prescription requirement, be medium with water, auxiliary agents such as former medicine, emulsifier, dispersant are joined in the batching still, mix through high-speed shearing machine and make aqueous emulsion, through after the assay was approved, measure packing and promptly obtain finished product.
Comparative example
Get 30% thifluzamide suspending agent (just reaching earlier Britain Investment Co., Ltd produces) and 12.5% epoxiconazole suspension concentrate (Jiangsu Gengyun Chemical Co., Ltd.'s production) respectively and organize in contrast, embodiment 1～9 is as experimental group, and reagent agent is all according to 50kg/667m 2Even spraying is prevented and treated rice sheath blight disease and strawberry anthracnose,, uses once every 7d with medicine for the first time in their early stage, respectively at 7d and 14d investigation field efficacy behind the second time medicine again.
The rice sheath blight disease grade scale is as follows:
0 grade---do not fall ill;
1 grade---the morbidity of first leaf sheath;
3 grades---first blade and the morbidity of second leaf sheath;
5 grades---second blade and the morbidity of second leaf sheath;
7 grades---whole strain morbidity.
Strawberry anthrax disease grade scale is:
0 grade: no scab;
1 grade: lesion area accounts for whole leaf area below 5%;
3 grades: lesion area accounts for whole leaf area 6%～15%;
5 grades: lesion area accounts for whole leaf area 16%～25%;
7 grades: lesion area accounts for whole leaf area 26%～50%;
9 grades: lesion area accounts for whole leaf area more than 51%.
Disease index=(morbidity number * graded index at different levels) * 100/ (investigation seedling number * 7)
Control efficiency (%)=(contrast disease index-processing disease index) * 100/ contrast disease index
Table 3 thifluzamide and fluorine ring azoles and different proportioning embodiment are to rice sheath blight disease and strawberry anthracnose field efficacy
As shown in Table 3, thifluzamide and fluorine ring azoles are mixed, and have the notable synergistic effect and enlarge the fungicidal spectrum effect.Wherein, 30% thifluzamide, 30% fluorine ring azoles are mixed with water dispersible granules, and the bactericidal effect of the suspending agent that is mixed with of 30% thifluzamide, 15% fluorine ring azoles is the most outstanding.
The above only is a preferred implementation of the present invention; be noted that for those skilled in the art; under the prerequisite that does not break away from the principle of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (1)

1. the application of bactericide on control strawberry anthracnose, it is characterized in that: this bactericide comprises thifluzamide and fluorine ring azoles, and the mass ratio of described thifluzamide and fluorine ring azoles is 2:5 ~ 5:2, and described thifluzamide and fluorine ring azoles account for 15 ~ 60% of gross mass.
CN 201110234198 2011-08-16 2011-08-16 Bactericide and application of bactericide in prevention and control of rice sheath blight and strawberry anthracnose Expired - Fee Related CN102258036B (en)

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CN102657185B (en) * 2012-04-20 2014-04-23 杭州宇龙化工有限公司 Sterilization combination containing thifluzamide and tetraconazole
CN103563919A (en) * 2012-08-04 2014-02-12 南京华洲药业有限公司 Bactericidal composition containing thifluzamide and triadimefon and application thereof
CN103563923B (en) * 2012-08-04 2015-06-03 南京华洲药业有限公司 Bactericidal composition containing thifluzamide and epoxiconazole and application thereof
CN102864220B (en) * 2012-08-09 2014-06-11 中国农业大学 Method for identifying nucleotide point mutation of rhizoctonia solani Sdh gene and drug resistance of rhizoctonia solani Sdh gene to thifluzamide
CN103060422B (en) * 2013-01-04 2014-11-05 杭州市农业科学研究院 Method for identifying whether strawberry to be anthracnose-resistant or not indoors

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Publication number Priority date Publication date Assignee Title
CN101816307A (en) * 2010-05-13 2010-09-01 江苏丘陵地区镇江农业科学研究所 Bactericide for preventing and treating fruit diseases
CN102239853A (en) * 2011-07-27 2011-11-16 南宁市德丰富化工有限责任公司 Thifluzamide-containing composite bacteriacide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101816307A (en) * 2010-05-13 2010-09-01 江苏丘陵地区镇江农业科学研究所 Bactericide for preventing and treating fruit diseases
CN102239853A (en) * 2011-07-27 2011-11-16 南宁市德丰富化工有限责任公司 Thifluzamide-containing composite bacteriacide

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