CN102250816A - Endophytic bacillus subtilis - Google Patents
Endophytic bacillus subtilis Download PDFInfo
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- CN102250816A CN102250816A CN 201110205587 CN201110205587A CN102250816A CN 102250816 A CN102250816 A CN 102250816A CN 201110205587 CN201110205587 CN 201110205587 CN 201110205587 A CN201110205587 A CN 201110205587A CN 102250816 A CN102250816 A CN 102250816A
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- bacillus subtilis
- endophytic bacillus
- endophytic
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Abstract
The invention specifically discloses an endophytic bacillus subtilis, which relates to the field of microbes. The endophytic bacillus subtilis provided in the invention is conserved in the CCCCM common micro-organism center and is assigned an accession number of CGMCC No.4844. The 40th generation of the endophytic bacillus subtilis still has high activity in inhibiting spores of phytophthora foot rot strains, high genetic stability, an effect on controlling sudden death disease similar to the effect of chemical controlling and no pollution to environment, is ecological and safe, and can be widely applied in controlling sudden death disease and in preparing preparations for controlling sudden death disease.
Description
Technical field
The present invention relates to microorganism field, relate to a kind of endophytic Bacillus subtilis in particular.
Background technology
Pepper (Piper nigrum L.) is a piperaceae Piper perennial evergreen climbing vine plant, is the important tropical characteristic spice crop in the world, and one of, favorite seasonings the most frequently used for people is of many uses in fields such as food, health cares.
The pepper seasonal febrile diseases are a kind of weather dependency diseases, take place when being everlasting rainy season, and it is the very strong soil-borne disease of a kind of infectivity that is caused by the phytophthora infection.Since introducing a fine variety pepper, the pepper seasonal febrile diseases just are the primary diseases that harm China pepper produces always, the pepper garden is with a toll of more than 90% when being in a bad way, even full garden is destroyed, bring immeasurable loss for China's pepper plant husbandry, China has carried out comparatively deep research to the Prevention Technique of pepper seasonal febrile diseases for this reason.
At present, the control of pepper seasonal febrile diseases is mainly relied on the chemical prevention technology, as spray metaxanin Propamocarb WP.Though chemical prevention can reach the better prevention effect, but the life-time service chemical pesticide not only can produce pesticide residue, reduce the pepper quality, but also can cause the resistance of pepper seasonal febrile diseases germ, cause the pepper seasonal febrile diseases that the gesture that increases the weight of is year by year arranged in recent years along with long-term frequent use of chemical pesticide.Therefore, effectively, safety, ecological prevent and treat the primary goal that the pepper seasonal febrile diseases just become pepper plant husbandry.
Biological control is a kind of technique means of preventing and treating the pepper seasonal febrile diseases that proposes present stage, it is an indicator with the strain of sudden death disease germ, filter out the microorganism that can suppress the strain of sudden death disease germ in from soil and carry out biological control, this method has effectively been avoided the resistance and the problem of environmental pollution that utilize the chemical prevention technology to be brought.Yet the composition monoid of sudden death disease germ strain is very complicated, and is indicator with typical case or the strain of single sudden death disease germ during existing screening, so the microorganism that filters out has certain limitation, its prevention effect can not show a candle to the chemical prevention effect.In addition, the existing microorganism that filters out is along with the increase of passage number, and its inhibition activity to pepper seasonal febrile diseases germ spore reduces gradually, and genetic stability is relatively poor.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of endophytic Bacillus subtilis, make the effect of this bacterial strain control pepper warm disease and the effect of chemical prevention pepper seasonal febrile diseases not have significant difference, and have higher genetic stability.
For achieving the above object, the invention provides a kind of endophyte that obtains that separates from the pepper blade, called after bacterial strain Z, the bacterial strain Z rounded edge of bacterium colony on the NA substratum is irregular, oyster white, and opaque, surface irregularity has gauffer, tarnish.Be cultured to 36h, thalline all exists with the form of hypopus gemma, and it is light yellow that bacterium colony is.The microscopic examination thalline is unicellular, shaft-like, and is single living or twin, sometimes chaining.Gram-positive, gemma oval, middle life, peritrichous.Methylene blue dyeing, the protoplasma uniform coloring.Litmus milk does not produce acid.Catalase, V.P. reaction, starch hydrolysis, nitrate reduction reaction, Citrate trianion utilization, gelatine liquefication are all positive.The utilization of M.R, phenylalanine deaminase test, urease test, propionic salt is all negative.Can grow containing on the NA substratum of 7%NaCl and pH5.7.In addition, analyze through the 16S DNA detection, 50 bacterial strains high with bacterial strain Z homology all are genus bacillus, and homology in conjunction with physiological and biochemical property, identifies that it is a subtilis up to 100%.
Endophytic Bacillus subtilis of the present invention has been deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 12nd, 2011, the address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.4844.
Go down to posterity the 1st generation of cultivating, the 10th generation, the 20th generation, the 30th generation, the 40th generation inhibition test to pepper seasonal febrile diseases germ spore of endophytic Bacillus subtilis of the present invention shows that described endophytic Bacillus subtilis suppresses the EC of pepper seasonal febrile diseases germ spore germination from 40 generations of the 1st generation to the
50No significant difference, and all below 5.5%, showing that endophytic Bacillus subtilis of the present invention has suppresses effect preferably, and genetic stability is higher, can not reduce the inhibition activity to pepper seasonal febrile diseases germ spore with the increase of passage number.
Endophytic Bacillus subtilis of the present invention and metaxanin Propamocarb WP show that to pepper seasonal febrile diseases efficiency test result both preventive effects are suitable, no significant difference.Show that endophytic Bacillus subtilis preventive effect of the present invention is higher, reach the effect of chemical prevention, can be applied in control pepper seasonal febrile diseases and the preparation control pepper seasonal febrile diseases preparation.
The present invention also provides a kind of biological prevention and control agent, comprises that deposit number is the endophytic Bacillus subtilis of CGMCC No.4844.Biological prevention and control agent of the present invention can use before pepper is transplanted in spraying, also can make the compost use, and perhaps the root use is irritated in the dilution back when transplanting.
The preparation method of described biological prevention and control agent of the present invention is as follows:
The endophytic Bacillus subtilis that with deposit number is CGMCC No.4844 is cultivated in the PDA nutrient solution, and filtering and regulate bacterium liquid spore count then is 10
8-10
10Individual/mL, promptly get the biological prevention and control agent finished product.
Wherein, the temperature of described cultivation is preferably 26-28 ℃, and more preferably 27 ℃, the pH value of described cultivation is preferably 7-9, and more preferably 8.
By above technical scheme as can be known, endophytic Bacillus subtilis of the present invention is passaged to the activity that 40 generations still had higher inhibition sudden death disease germ strain spore, has higher genetic stability, the effect of its control pepper seasonal febrile diseases reaches the effect of chemical prevention simultaneously, and it is free from environmental pollution, ecological, safety can be widely used in the control and preparation control pepper seasonal febrile diseases preparation of pepper seasonal febrile diseases.
Biological preservation explanation
Classification name: subtilis, Bacillus subtilis. is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 12nd, 2011, the address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.4844.
Embodiment
The invention discloses a kind of endophytic Bacillus subtilis, those skilled in the art can use for reference this paper content, suitably improve processing parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as being included in the present invention.Endophytic Bacillus subtilis of the present invention is described by preferred embodiment, the related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use the technology of the present invention.
Below in conjunction with embodiment, further set forth the present invention.
Embodiment 1: the separation method of endophytic Bacillus subtilis of the present invention
Heavily adopt the long healthy blade of bringing back to life in the district from the morbidity of pepper seasonal febrile diseases, be cut into the tissue block of about 2cm * 2cm specification with the aseptic operation cutter, aseptic water washing for several times, in 75% alcohol, soak 1min, aqua sterilisa flushing 3 times places aseptic mortar with tissue block, adds sterilized water after shredding with scissors and grinds, leave standstill 30min, be diluted to 10
-1, 10
-2, 10
-33 gradients pipette last 1 washing fluid and each 0.3mL of lapping liquid respectively, are applied on the PDA culture medium flat plate, and last 1 washing fluid is cultivated down in 28 ℃ as CK, observes day by day.Selecting does not have bacterium colony among the CK, and grows the bacterial strain of bacterium colony in the lapping liquid, and according to feature picking list bacterium colonies such as colonial morphology, colors, after the purifying numbering, face-off is cultivated and is detected its restraining effect to pepper seasonal febrile diseases germ in plate, selects to form the bacterial strain of antibacterial band, called after bacterial strain Z.
Embodiment 2: the mensuration of endophytic Bacillus subtilis Physiology and biochemistry of the present invention and 16S dna sequence dna
1, Physiology and biochemistry detects
Detect growing state, colonial morphology and color, gramstaining, spore staining and the flagella staining etc. of bacterial strain Z on the NA flat board, the method that " the planting the disease research method " that method is edited with reference to Fang Zhongda is 182 pages is carried out.
Detect bacterial strain Z physio-biochemical characteristics, comprise: the utilization of litmus milk, catalase, V.P. reaction, starch hydrolysis, nitrate reduction reaction, gelatine liquefication reaction, oxydase, M.R, phenylalanine deaminase, Citrate trianion utilization, urease test, propionic salt, test such as whether can grow on the substratum that contains 7%NaCl and pH5.7, method is with reference to the method for " common bacteria system identification handbook " 353-385 page or leaf of the elegant pearl in east, Cai Miaoying chief editor.
Detected result is as follows:
The rounded edge of bacterium colony is irregular on the NA substratum, and oyster white is opaque, and surface irregularity has gauffer, tarnish.Be cultured to 36h, thalline all exists with the form of hypopus gemma, and it is light yellow that bacterium colony is.The microscopic examination thalline is unicellular, shaft-like, and is single living or twin, sometimes chaining.Gram-positive, gemma oval, middle life, peritrichous.Methylene blue dyeing, the protoplasma uniform coloring.Litmus milk does not produce acid.Catalase, V.P. reaction, starch hydrolysis, nitrate reduction reaction, Citrate trianion utilization, gelatine liquefication are all positive.The utilization of M.R, phenylalanine deaminase test, urease test, propionic salt is all negative.Can grow containing on the NA substratum of 7%NaCl and pH5.7.
2, the mensuration of 16S dna sequence dna
With reference to the bacterial genomes DNA preparation method of Ausubel, carry out pcr amplification with bacterium universal primer 27F and 1492R.
The PCR reaction system is 25 μ l, 10 * PCR Buffer, 2.5 μ l wherein, each 1 μ l of Primer27F and Primer 1492R (25pmoL), dNTP Mixture 0.5 μ l, Taq DNA Polymerase (5U/ μ l) 0.5 μ l, ddH
2O 17.5 μ l, dna profiling 2 μ l.
Pcr amplification reaction condition: 95 ℃ of 4min; 95 ℃ of 1min, 55 ℃ of 40s, 72 ℃ of 2min, totally 30 circulations; 72 ℃ keep 7min then.The PCR product reclaims test kit with EZ-10 SpinColum DNA Gel Extraction Kit and reclaims, and entrusts precious biotechnology (Dalian) company limited to check order.The gene order that records is carried out the sequence alignment analysis with BLAST software, and with GenBank in known 16S rDNA carry out homology relatively.
Detected result is as follows:
With this pcr amplification product (accession number: JF747222) carry out the BLAST comparison in GenBank.The result shows, 50 bacterial strains high with Z bacterial strain homology all are bacillus (Bacillus sp.), its homology reaches 100%, and wherein table 1 is depicted as 10 bacterial strains the highest with the 16S rDNA homology of bacterial strain Z, and deducibility bacterial strain Z belongs to bacillus (Bacillus sp.) thus.
Table 116S rDNA homology relatively
| The sequence accession number | Bacterial strain | Homology |
| HM107809.1 | Bacillus amyloliquefaciens strain 16 | 100％ |
| HM107806.1 | Bacillus amyloliquefaciens strain 13 | 100％ |
| HM224387.1 | Bacillus subtilis strain M2 | 100％ |
| HM055610.1 | Bacillus amyloliquefaciens strain SD1b | 100％ |
| FJ644629.1 | Bacillus subtilis strain AQ1 | 100％ |
| GU826164.2 | Bacillus subtilis strain BL9 | 100％ |
| GU826163.1 | Bacillus subtilis strain BL8 | 100％ |
| GU826162.1 | Bacillus subtilis strain BL7 | 100％ |
| GU826161.1 | Bacillus subtilis strain BL6 | 100％ |
| GU568203.1 | Bacillus amyloliquefaciens strain GBSW11 | 100％ |
In conjunction with Physiology and biochemistry and 16S rDNA detected result, bacterial strain Z is accredited as subtilis (Bacillus subtilis.).
Embodiment 3: endophytic Bacillus subtilis of the present invention is to the inhibition test of pepper seasonal febrile diseases germ spore germination
In the PDA culture dish, 28 ℃ of cultivations are changeed ware 1 time every 24h, corotation 40 times with activatory endophytic Bacillus subtilis streak inoculation of the present invention.Measure the inhibition effect of the endophytic Bacillus subtilis bacterial strain in the 1st generation, 10 generations, 20 generations, 30 generations and 40 generations with sessile drop method, the results are shown in Table 2 the spore germination of pepper seasonal febrile diseases germ.
Table 2 endophytic Bacillus subtilis of the present invention is to the inhibition test of pepper seasonal febrile diseases germ spore germination
By table 2 result as can be known, endophytic Bacillus subtilis of the present invention suppresses the EC of pepper seasonal febrile diseases germ spore germination from the bacterium liquid in 40 generations of the 1st generation to the
50Difference is very little, shows that endophytic Bacillus subtilis of the present invention can not reduce the inhibition activity to pepper seasonal febrile diseases germ spore with the increase of passage number, has higher genetic stability.Simultaneously, endophytic Bacillus subtilis bacterium liquid of the present invention suppresses the EC of pepper seasonal febrile diseases germ spore germination
50Below 5.5%, show that it has good inhibition spore germination effect.
Embodiment 4: endophytic Bacillus subtilis of the present invention is to the efficiency test of pepper seasonal febrile diseases
3 processing are established in test: (spore count is 10 to endophytic Bacillus subtilis bacterium liquid of the present invention
8Individual/mL) 500 times of diluents; 1000 times of diluents of commercially available 25% metaxanin Propamocarb WP; Clear water.
The mensuration of provide protection: spray the soup of 3 processing earlier on the potted plant seedling of pepper, whole strain all squirts, with the soup degree of being that drips.Under the condition of preserving moisture, inoculate sudden death disease disease pathogen bacterium behind the 24h.Each is handled 3 times and repeats, 10 seedlings of every repetition, 3 leaves of every seedling inoculation.Carry out state of an illness investigation by table 3 grade scale behind the 5d.
The mensuration of therapeutic action: inoculate sudden death disease disease pathogen bacterium under the condition of preserving moisture, spray the soup of 3 processing behind the 24h on potted plant seedling, whole strain all squirts, with the soup degree of being that drips.Each is handled 3 times and repeats, 10 seedlings of every repetition, 3 leaves of every seedling inoculation.Carry out state of an illness investigation by table 3 grade scale behind the 5d.
Table 3 degree of being in a bad way grade scale
| 0 grade | No scab |
| 1 grade | Lesion area accounts for below 1/8 of one-piece blade area |
| 3 grades | Lesion area accounts for the 1/8-1/4 of one-piece blade area |
| 5 grades | Lesion area accounts for the 1/4-1/2 of one-piece blade area |
| 7 grades | Lesion area accounts for more than 1/2 of one-piece blade area |
Wherein, disease index=∑ (the sick numbers of sheets at different levels * this disease level level value)/(investigation leaf sum * the highest sick level value) * 100; prevention effect (%)=(contrast disease index-processing disease index)/contrast disease index * 100%; variance analysis is carried out in the provide protection of statistics and the anti-effectiveness SAS software of therapeutic action; the preventive effect of clear water contrast is with 0 note, and the result sees Table 4 and table 5 respectively.
The preventive effect of table 4 endophytic Bacillus subtilis provide protection of the present invention
The preventive effect of table 5 endophytic Bacillus subtilis therapeutic action of the present invention
By table 4 and table 5 result as can be known; endophytic Bacillus subtilis of the present invention reaches 77.82% to the preventive effect of pepper seasonal febrile diseases provide protection; the preventive effect of therapeutic action is 78.18%, and is suitable with the chemical prevention effect of carrying out with 25% metaxanin Propamocarb WP, no significant difference.
Embodiment 5: prepare biological prevention and control agent of the present invention
The endophytic Bacillus subtilis that with deposit number is CGMCC No.4844 is cultivated in the PDA nutrient solution, and culture temperature is 27 ℃, and fate is 5d, and rotating speed is 200rpm, and the pH value is 8, and filtering and regulate bacterium liquid spore count then is 10
9Individual/mL, promptly get the biological prevention and control agent finished product.
Embodiment 6: prepare biological prevention and control agent of the present invention
The endophytic Bacillus subtilis that with deposit number is CGMCC No.4844 is cultivated in the PDA nutrient solution, and culture temperature is 28 ℃, and fate is 5d, and rotating speed is 200rpm, and the pH value is 9, and filtering and regulate bacterium liquid spore count then is 10
10Individual/mL, promptly get the biological prevention and control agent finished product.
Embodiment 7: prepare biological prevention and control agent of the present invention
The endophytic Bacillus subtilis that with deposit number is CGMCC No.4844 is cultivated in the PDA nutrient solution, and culture temperature is 26 ℃, and fate is 5d, and rotating speed is 200rpm, and the pH value is 7, and filtering and regulate bacterium liquid spore count then is 10
8Individual/mL, promptly get the biological prevention and control agent finished product.
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. an endophytic Bacillus subtilis is characterized in that, deposit number is CGMCC No.4844.
2. the application of the described endophytic Bacillus subtilis of claim 1 in control pepper seasonal febrile diseases.
3. the application of the described endophytic Bacillus subtilis of claim 1 in preparation control pepper seasonal febrile diseases preparation.
4. a biological prevention and control agent is characterized in that, comprises that deposit number is the endophytic Bacillus subtilis of CGMCC No.4844.
5. the application of the described biological prevention and control agent of claim 4 in control pepper seasonal febrile diseases.
6. the preparation method of a biological prevention and control agent is characterized in that, is that the endophytic Bacillus subtilis of CGMCC No.4844 is cultivated in the PDA nutrient solution with deposit number, and filtering and regulate bacterium liquid spore count then is 10
8-10
10Individual/mL, promptly get the biological prevention and control agent finished product.
7. according to the described preparation method of claim 6, it is characterized in that the temperature of described cultivation is 26-28 ℃.
8. according to the described preparation method of claim 7, it is characterized in that the temperature of described cultivation is 27 ℃.
9. according to the described preparation method of claim 6, it is characterized in that the pH value of described cultivation is 7-9.
10. according to the described preparation method of claim 9, it is characterized in that the pH value of described cultivation is 8.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107760639A (en) * | 2017-12-05 | 2018-03-06 | 中国热带农业科学院香料饮料研究所 | Bacterial strain and its application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1310229A (en) * | 2000-12-14 | 2001-08-29 | 中国科学院新疆生态与地理研究所 | PR5 bacteria preparation to inhibit phytophthora disease |
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
| US20070190591A1 (en) * | 2001-05-02 | 2007-08-16 | Grech Nigel M | Microbial compositions and methods of use in water |
-
2011
- 2011-07-21 CN CN 201110205587 patent/CN102250816B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1310229A (en) * | 2000-12-14 | 2001-08-29 | 中国科学院新疆生态与地理研究所 | PR5 bacteria preparation to inhibit phytophthora disease |
| US20070190591A1 (en) * | 2001-05-02 | 2007-08-16 | Grech Nigel M | Microbial compositions and methods of use in water |
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
Non-Patent Citations (2)
| Title |
|---|
| 《2003'华东植物病理学术研讨会论文集》 20031231 邱思鑫 "植物内生促生防病细菌的筛选" , * |
| 《WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY》 20100731 Todorova, S "Characteristics and antimicrobial activity of Bacillus subtilis strains isolated from soil" 第26卷, 第7期 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107760639A (en) * | 2017-12-05 | 2018-03-06 | 中国热带农业科学院香料饮料研究所 | Bacterial strain and its application |
| CN107760639B (en) * | 2017-12-05 | 2020-03-31 | 中国热带农业科学院香料饮料研究所 | Bacterial strain and application thereof |
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