CN1310229A - PR5 bacteria preparation to inhibit phytophthora disease - Google Patents
PR5 bacteria preparation to inhibit phytophthora disease Download PDFInfo
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- CN1310229A CN1310229A CN 00135652 CN00135652A CN1310229A CN 1310229 A CN1310229 A CN 1310229A CN 00135652 CN00135652 CN 00135652 CN 00135652 A CN00135652 A CN 00135652A CN 1310229 A CN1310229 A CN 1310229A
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- microbial inoculum
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- root rot
- phytophthora
- bacillus
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Abstract
PR5 bacteria preparation is obtained through separation and screen of soil around roots of ephedra as one kind of syrtidad and belongs to, identification shows, Bacillus. It is used in dressing seed or applying to root, so that PR5 bacterial colony propagates around roots massively and secretes toxoprotein to inhibit phytophthora and treat phytophthora disease. It has obvious inhibition effect on phytophthora disease of solanaceous vegetables, water melon, chilli, etc. Experiments show that it has bacteriostasis rate of 71.4-87.5 % and has certain effect of promoting growth of vegetable and melon.
Description
The present invention relates to a kind of PR that suppresses phytophthora root rot
5Microbial inoculum and preparation method thereof
Phytophthora root rot distributes very extensive in China vegetables producing region, and is one of very big soilborne disease of hazardness.This disease velocity of propagation is very fast, in case find the phytophthora root rot state of an illness, administers day after the fair again.The early prevention thing must gone, and adopting biological control should be optimal selection.
At present, utilize rhizospheric microorganism to carry out the focus that biological control has become research and development.Antagonistic microbe is to the biological regulating and controlling effect and the existing many reports of mechanism thereof of soil-borne disease.If have the microorganism species of antagonism pathogenic bacteria occupies an leading position at crop rhizosphere, crop produces materials such as antagonist protein or microbiotic in metabolic process so, form the barrier of opposing soil-borne disease pathogenic bacteria infringement, just can effectively suppress development and the propagation of the pathogenic bacteria in the soil, reach the purpose of early prevention.
The object of the invention is, the PR of the inhibition phytophthora root rot of development
5Microbial inoculum is the microbial inoculum by separation screening in the arid psammophyte racephedrine rhizosphere soil.Should belong to bacillus through identifying.PR
5Bacterium is through the PR of fermentation system
5Microbial inoculum by measures such as the root of dressing seed, be stained with, filling roots, makes PR
5Flora enters crop root zone and breeds in a large number at rhizosphere colonization, occupies main site in the fauna of root.PR is provided in the environment of enough nutrient, air and moisture at root system and soil
5Bacterium will be constantly growth and breeding, the secretion toxalbumin suppresses the generation of elicitin pathogenic bacteria and to the infecting of root system, thereby reaches the purpose of control phytophthora root rot.This microbial inoculum has significant inhibitory effect to the phytophthora root rot of solanaceous vegetable and watermelon etc.Suppress the sick effect R of Phytophthora capsici
2/ R
1Be 0.47 comparatively obvious.With the potted plant bacteriostatic test PR that does
5The microbial inoculum bacteriostasis rate reaches 87.5%, and bacteriostatic test PR is made in the field
5Bacteriostasis rate reaches 71.4%.Except that the phytophthora root rot of inhibition is arranged,, can improve output more than 20% to vegetables and the melon effect that has promotion to grow.
The PR of inhibition phytophthora root rot of the present invention
5Microbial inoculum, it is the bacterium by separation screening in the arid biogeographic zone psammophyte racephedrine rhizosphere soil, belongs to bacillus, all at China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation (CGMCC), NO.0523
The PR that suppresses phytophthora root rot
5The preparation method of microbial inoculum follows these steps to carry out:
Former spawn culture method: PR
5The substratum that microbial inoculum is used is bacillus substratum commonly used (1000 milliliters are radix), and extractum carnis 2-8 gram, peptone 2-8 gram are with 1000 milliliters of 7 ° of wort constant volumes, the agar powder that adds 1.0-2.0% during solid culture, transfer PH 7, aerobic cultivation, culture temperature 28-32 ℃ with sodium hydroxide.
Liquid fermenting: bacterial classification is carried out slant strains cultivate, insert the triangular flask liquid culture again and insert the seeding tank aerated culture, air pressure is 0.01-0.05 μ Pa, 18 hours time entered fermentor tank, 24 hours time, leavening temperature 28-30 ℃, can put jar, packing, packing.
The PR of inhibition phytophthora root rot of the present invention
5Microbial inoculum, wherein PR
5Bacterium is characterized as bacterium, rod-short, and 2.5 * 1.2mm, peritrichous has brood cell, middle life; Gram-positive; The catalase reacting positive; The nitrate reaction positive.Glucose utilization produces acid, aerogenesis not; The VP reacting positive; The starch hydrolysis positive; Citric acid utilizes positive; The casein hydrolysis positive; The tyrosine hydrolysis feminine gender.According to PR
5The feature of microbial inoculum is checked by uncle's outstanding bacterium handbook (eight editions) index, should name to be subtilis (Bacillius subtilis).
PR
5The microbial inoculum standard: with the viable count in the microbial inoculum is leading indicator, reaches 25-30 hundred million/ml, and zymotechnique all carries out under aseptic condition, and other assorted bacterium must not be arranged in the microbial inoculum.Detection method: recording the result with dull and stereotyped dilution process is main foundation.In making processes, be foundation mainly with microscope blood counting method.
Field effect: PR
5Microbial inoculum is mainly used in vegetables and melon plant husbandry, and vegetables and melon phytophthora root rot are had restraining effect, and the sick inhibiting rate of Phytophthora capsici is reached more than 60%, and watermelon phytophthora root rot inhibiting rate is reached more than 70%.
Using method:
Be stained with root or irritate root: PR
520 times of microbial inoculum dilutions make every milliliter of bacterium liquid contain the bacterium number and reach more than 100,000,000.Root was stained with in the average 5ml/ strain of consumption when the dish seedling was transplanted, and irritated root in the time of plant strain growth 20-30 centimetre, and dilution is 40 times when irritating root, irritates the average 10ml/ strain of root consumption.
Spray: took place preceding 10 days pressing down mildew, 100 times of microbial inoculum dilutions, every mu of ground preferably sprays to the rhizome position when spraying with 10 kilograms (dilutions).
Seed dressing: watermelon, vegetables etc. are dressed seed prior to seeding and get final product every mu of former PR of grain weight
5Microbial inoculum liquid 300ml seed dressing is deposited to sow after 30 minutes and is got final product.
Embodiment 1
(1) at first prepares PR
5Microbial inoculum
Former spawn culture: PR
5The substratum that microbial inoculum is used is bacillus substratum commonly used (1000 milliliters are radix), and extractum carnis 2 grams, peptone 8 grams are with 1000 milliliters of 7 ° of wort constant volumes, the agar powder that adds 1.0-2.0% during solid culture, transfer PH7 with sodium hydroxide, aerobic cultivation, culture temperature 28-32 ℃.
Liquid fermenting: bacterial classification is carried out slant strains cultivate, insert the triangular flask liquid culture again and insert the seeding tank aerated culture, air pressure is 0.01-0.05 μ Pa, 18 hours time; Enter fermentor tank, 24 hours time, leavening temperature 28-30 ℃, can put jar, packing, packing.
(2) using method:
April, at first select the sick region of disease of Phytophthora capsici, 1.3 mu of lands used, 0.65 mu is contrast, 0.65 mu is to handle in addition.Be stained with root, consumption 5ml/ strain with the bacterium liquid that dilutes 20 times during pepper cultivation.Be stained with plantation in root 5-10 minute.Cross the two weeks investigation after transplanting seedlings, the rice shoot growing way is normal, and contrast does not all have notable difference with processing.Mid-July, the phytophthora root rot emergence period is carried out the investigation second time.Handle with contrast and select 10 points respectively at random, every 40 strain contrasts and handles and investigate 400 strains respectively.Contrast morbidity 52 strains, sickness rate is 13%; PR
5The microbial inoculum treatment zone, 16 strains of falling ill, sickness rate is 4%; PR
5It is 69.2% that microbial inoculum presses down the disease rate.In September, investigation is for the third time surveyed and is produced, and handles comparison according to volume increase 36.2%.
Embodiment 2
(1) at first prepares PR
5Microbial inoculum
Former spawn culture: PR
5The substratum that microbial inoculum is used is bacillus substratum commonly used (1000 milliliters are radix), and extractum carnis 8 grams, peptone 2 grams are with 1000 milliliters of 7 ° of wort constant volumes, the agar powder that adds 1.0-2.0% during solid culture, transfer PH7 with sodium hydroxide, aerobic cultivation, culture temperature 28-32 ℃.
Liquid fermenting: bacterial classification is carried out slant strains cultivate, insert the triangular flask liquid culture again and insert the seeding tank aerated culture, air pressure is 0.01-0.05 μ Pa, 18 hours time; Enter fermentor tank, 24 hours time, leavening temperature 28-30 ℃, can put jar, packing, packing.
(2) using method:
April, at first select the phytophthora root rot region of disease, select 4 mu of slight phytophthora root rot spots, divide two sub-districts, 2 mu of every sub-districts.Distinguish black close No. 2 watermelons of a kind of no seed, distinguish 2 kinds of yellow watermelons.Every sub-district is divided contrast and is handled each 0.07 hectare.Before the sowing, treatment zone 300mlPR
5Sowing after 30 minutes is deposited in microbial inoculum (stoste) seed dressing.Contrast is sowed with 300ml clear water seed dressing back.Contrast is identical with other Agricultural management methods of handling.Investigate seedling stage at watermelon, the treatment zone growing way is better than contrast, and leaf is dark green, and stem is sturdy.The melonry phytophthora root rot emergence period is carried out the investigation second time, 10 of the contrast of every sub-district and processing difference random selecting points, and each some investigation 20 strain adds up to 200 strains.In district's 1 contrast, 200 strains 28 strains morbidity is arranged, the morbidity strain accounts for 14%.Treatment zone 200 strains have 6 strains morbidity, and sickness rate is 3%, PR
5The inhibiting rate of the anti-phytophthora root rot of microbial inoculum reaches 78.8%; In district's 2 contrasts, 200 strains 25 strains morbidity is arranged, sickness rate is 12.5%.Handle morbidity 5 strains in 200 strains, sickness rate 2.5%.PR
5It is 84% that microbial inoculum presses down the disease rate.User's statistics was distinguished 1 and was used PR September
5The aspermous watermelon comparison that microbial inoculum is handled is according to volume increase 25%.Distinguish 2 yellow watermelons and shine volume increase 30% with the comparison of PR5 microbial inoculum processing.
Embodiment 3
(1) at first prepares PR
5Microbial inoculum
Former spawn culture: PR
5The substratum that microbial inoculum is used is bacillus substratum commonly used (1000 milliliters are radix), and extractum carnis 6 grams, peptone 4 grams are with 1000 milliliters of 7 ° of wort constant volumes, the agar powder that adds 1.0-2.0% during solid culture, transfer PH7 with sodium hydroxide, aerobic cultivation, culture temperature 28-32 ℃.
Liquid fermenting: bacterial classification is carried out slant strains cultivate, insert the triangular flask liquid culture again and insert the seeding tank aerated culture, air pressure is 0.01-0.05 μ Pa, 18 hours time; Enter fermentor tank, 24 hours time, leavening temperature 28-30 ℃, can put jar, packing, packing.
(2) using method:
At first the capsicum base slight generating area of phytophthora root rot is tested, 2 mu of choosing ground areas, 1 mu of contrast, PR is made on 1 mu of ground
5Microbial inoculum is handled, and uses 5ml/ strain PR during the capsicum transplantation of seedlings
5Bacterium liquid is stained with root, plantation after 10 minutes.The sick emergence period investigation of Phytophthora capsici.Contrast and use PR
5That handles selects 10 points respectively at random, every some investigation 88 strains, and contrasting has 55 strains morbidity, sickness rate 6.25% in 880 strains; Use PR
55 strains morbidity is only arranged, sickness rate 0.34% in 880 strains that microbial inoculum is handled; PR
5Microbial inoculum presses down the disease rate and reaches 94%.According to customer responsiveness, use PR
5The capsicum that microbial inoculum is stained with root prolongs breeding time, and contrast ground is withered substantially, does not tie the green pepper phenomenon.And PR
5Microbial inoculum still has a lot of fresh chillis to pluck with handling capsicum.
Claims (2)
1, a kind of PR that suppresses phytophthora root rot
5Microbial inoculum is characterized in that it is a bacterium by separation screening in the arid biogeographic zone psammophyte racephedrine rhizosphere soil, belongs to bacillus, all at China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation (CGMCC), NO.0523
2, a kind of PR that suppresses phytophthora root rot
5The preparation method of microbial inoculum is characterized in that following these steps to carrying out:
Former spawn culture method: PR
5The substratum that microbial inoculum is used is bacillus substratum commonly used (1000 milliliters are radix), and extractum carnis 2-8 gram, peptone 2-8 gram are with 1000 milliliters of 7 ° of wort constant volumes, the agar powder that adds 1.0-2.0% during solid culture, transfer PH7 with sodium hydroxide, aerobic cultivation, culture temperature 28-32 ℃.
Liquid fermenting: bacterial classification is carried out slant strains cultivate, insert the triangular flask liquid culture again and insert the seeding tank aerated culture, air pressure is 0.01-0.05 μ Pa, 18 hours time entered fermentor tank, 24 hours time, leavening temperature 28-30 ℃, can put jar, packing, packing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00135652 CN1116412C (en) | 2000-12-14 | 2000-12-14 | PR5 bacteria preparation to inhibit phytophthora disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00135652 CN1116412C (en) | 2000-12-14 | 2000-12-14 | PR5 bacteria preparation to inhibit phytophthora disease |
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| Publication Number | Publication Date |
|---|---|
| CN1310229A true CN1310229A (en) | 2001-08-29 |
| CN1116412C CN1116412C (en) | 2003-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00135652 Expired - Fee Related CN1116412C (en) | 2000-12-14 | 2000-12-14 | PR5 bacteria preparation to inhibit phytophthora disease |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100334953C (en) * | 2003-11-22 | 2007-09-05 | 西北农林科技大学 | Preparation for biological prevention and cure of hot pepper epidemic disease and method for producing the same |
| CN102250816A (en) * | 2011-07-21 | 2011-11-23 | 中国热带农业科学院香料饮料研究所 | Endophytic bacillus subtilis |
| CN109628344A (en) * | 2018-12-28 | 2019-04-16 | 天津开发区坤禾生物技术有限公司 | A kind of prevention and treatment Kiwi berry phytophthora root rot microbial inoculum and its preparation method and application |
-
2000
- 2000-12-14 CN CN 00135652 patent/CN1116412C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100334953C (en) * | 2003-11-22 | 2007-09-05 | 西北农林科技大学 | Preparation for biological prevention and cure of hot pepper epidemic disease and method for producing the same |
| CN102250816A (en) * | 2011-07-21 | 2011-11-23 | 中国热带农业科学院香料饮料研究所 | Endophytic bacillus subtilis |
| CN102250816B (en) * | 2011-07-21 | 2013-02-13 | 中国热带农业科学院香料饮料研究所 | Endophytic bacillus subtilis |
| CN109628344A (en) * | 2018-12-28 | 2019-04-16 | 天津开发区坤禾生物技术有限公司 | A kind of prevention and treatment Kiwi berry phytophthora root rot microbial inoculum and its preparation method and application |
| CN109628344B (en) * | 2018-12-28 | 2021-07-09 | 天津开发区坤禾生物技术有限公司 | Bacterial agent for preventing and treating phytophthora root rot of kiwi fruits as well as preparation method and application of bacterial agent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1116412C (en) | 2003-07-30 |
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