CN102247425B - Composition of traditional Chinese medicine extract for treating gastrointestinal dysfunction and preparation method thereof - Google Patents
Composition of traditional Chinese medicine extract for treating gastrointestinal dysfunction and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a composition of a traditional Chinese medicine extract for treating gastrointestinal dysfunction and a preparation method thereof. The composition is characterized by consisting of the following components in parts by weight: 1-3 parts of large-head atractylodes rhizome extract, 1-3 parts of Indian buead extract, 1-2 parts of ginseng extract and 1-2 parts of licorice root extract, wherein the extracts are obtained by crushing Chinese medicinal crops into coarse powder and extracting by using a carbon dioxide supercritical extraction method. In the composition, the active ingredients are fully extracted, and the pharmacological effect is obviously superior to that of a single component extract and is superior to those of a carbon dioxide supercritical extract of a complete recipe, a conventionally applied volatile oil component and water extraction component composition or a volatile oil component and ethanol extraction component composition.
Description
Technical field
The invention belongs to the field of Chinese medicines, relate to a kind of composition and method of making the same for the treatment of the Chinese medicine extract of gastrointestinal dysfunction.
Background technology
Gastrointestinal dysfunction is a kind of clinical common functional intestinal tract disease, accounts for 30% of gastroenteropathy, especially take the young people as common.The prevalence of gastrointestinal dysfunction in the adult is 10%~22%, and the women is slightly common.Its main clinical manifestation is the gastrointestinal symptoms such as stomachache, diarrhoea, constipation.Its cause of disease is functional gastrointestinal disorder.Along with the quickening of people's rhythm of life, this disease prevalence is the trend of rising.The Therapeutic Method that adopts at present mainly contains the psychotherapy, Diet Therapy and Drug therapy.Because modern medicine is not clear and definite yet for its pathology and pathogenesis, make the chemical drugs treatment be absorbed in passive.Though the chemical drugs tool curative effect of listing, often accompany certain side effect at present, clinical efficacy is undesirable.Chinese medicine thinks that this disease is depression of liver-QI, due to dysfunction of the spleen in transportation.Decoction of four noble drugs comes from " formulary of peaceful benevolent dispensary ", and the side is comprised of Radix Ginseng, the Rhizoma Atractylodis Macrocephalae, Poria, Radix Glycyrrhizae four flavor Chinese medicines, cures mainly deficiency of spleen-QI and stomach-QI.The party is the square foundation for the treatment of syndrome of deficiency of spleen qi and stomach qi, and four medicines match, can spleen reinforcing soil and eliminating pathogen in the liver is wooden, and the regulating QI machine rushes down with pain relieving.Be used for the treatment of borborygmus diarrhoea, the stool diarrhea is rushed down all diseases of rear stomachache.Modern study finds that our decoction has the effect of Gastrointestinal motility adjustment, can suppress gastrointestinal propulsion, alleviating diarrhoea; Can make again the small intestinal that motion reduces recover normal.
But, the decoction that traditional handicraft prepares, the patient not only uses inconvenience, and is unfavorable for keeping unsettled effective ingredient in medicine.
Summary of the invention
The objective of the invention is provides a kind of compositions that is used for the treatment of the Chinese medicine extract of gastrointestinal dysfunction in order to overcome above-mentioned weak point.
Another object of the present invention is to provide the preparation method of the compositions of this Chinese medicine extract that is used for the treatment of gastrointestinal dysfunction.
The objective of the invention is to implement in the following manner:
A kind of compositions for the treatment of the Chinese medicine extract of gastrointestinal dysfunction is characterized in that said composition is composed of the following components: Rhizoma Atractylodis Macrocephalae extract 1~3 weight portion; Poria extract 1~3 weight portion; Radix Ginseng extract 1~2 weight portion; Radix Glycyrrhizae extract 1~2 weight portion; Described extract adopts carbon dioxide supercritical extraction method to extract for Chinese medicinal material is ground into coarse powder.
Described compositions is preferably composed of the following components: Rhizoma Atractylodis Macrocephalae extract 1 weight portion; Poria extract 1 weight portion; Radix Ginseng extract 1 weight portion; Radix Glycyrrhizae extract 1 weight portion; Described extract adopts carbon dioxide supercritical extraction method to extract for Chinese medicinal material is ground into coarse powder.
The compositions of the Chinese medicine extract for the treatment of gastrointestinal dysfunction of the present invention prepares by following steps:
The Rhizoma Atractylodis Macrocephalae, Poria, Radix Ginseng and Radix Glycyrrhizae are ground into respectively coarse powder, adopt respectively the carbon dioxide supercritical fluid extraction device to extract, extracting pressure 25~34MPa, 28~54 ℃ of extraction temperature, be extracted to without the extract generation, obtain Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract; Each extract is mixed in proportion, and get final product.
The compositions of the Chinese medicine extract of above-mentioned treatment gastrointestinal dysfunction specifically prepares by following steps:
(1) Rhizoma Atractylodis Macrocephalae is ground into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 31~34MPa, and 40~43 ℃ of extraction temperature, extraction time 80-90min collects Rhizoma Atractylodis Macrocephalae extract;
(2) Poria powder is broken into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 22~25MPa, and 28~32 ℃ of extraction temperature, extraction time 160-180min collects Poria extract;
(3) Radix Ginseng powder is broken into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 26~30MPa, 45~48 ℃ of extraction temperature, extraction time 180-200min, collector's conopsea extraction;
(4) Radix Glycyrrhizae powder is broken into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 24~27MPa, and 51~54 ℃ of extraction temperature, extraction time 200-220min collects Radix Glycyrrhizae extract;
(5) each extract is mixed in proportion, and get final product.
Chinese medicine composition of the present invention can add the adjuvant of pharmacy regulation be prepared into oral formulations, non-intestinal drug delivery agent, mucocutaneous drug-delivery preparation or suck preparation.As tablet, capsule, granule, drop pill, micropill, suspensoid, mixture, injection, aerosol, spray, inhalant, ointment, cataplasma, suppository, Emulsion, gel etc.
The preparation method of the Chinese medicine composition for the treatment of gastrointestinal dysfunction of the present invention comprises the following steps:
The Rhizoma Atractylodis Macrocephalae, Poria, Radix Ginseng and Radix Glycyrrhizae are ground into respectively coarse powder, adopt respectively the carbon dioxide supercritical fluid extraction device to extract, extracting pressure 25~34MPa, 28~54 ℃ of extraction temperature, be extracted to without the extract generation, obtain Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract; Each extract is mixed in proportion, and get final product.
The preparation method of the compositions of above-mentioned Chinese medicine extract specifically comprises the following steps:
(1) Rhizoma Atractylodis Macrocephalae is ground into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 31~34MPa, and 40~43 ℃ of extraction temperature, extraction time 80-90min collects Rhizoma Atractylodis Macrocephalae extract;
(2) Poria powder is broken into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 22~25MPa, and 28~32 ℃ of extraction temperature, extraction time 160-180min collects Poria extract;
(3) Radix Ginseng powder is broken into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 26~30MPa, 45~48 ℃ of extraction temperature, extraction time 180-200min, collector's conopsea extraction;
(4) Radix Glycyrrhizae powder is broken into coarse powder, and coarse powder adopts carbon dioxide supercritical fluid extraction, extracting pressure 24~27MPa, and 51~54 ℃ of extraction temperature, extraction time 200-220min collects Radix Glycyrrhizae extract;
(5) each extract is mixed in proportion, and get final product.
Compositions of the present invention is used in the medicine of preparation treatment gastrointestinal dysfunction.
The present invention adopts CO
2The advantage of supercritical extraction:
(1) can be near room temperature and CO
2Gas extracts under existing, and has effectively prevented oxidation and the loss of heat-sensitive substance; The complete retains biological activity of energy, and can be high boiling point, the material of low volatility, easily pyrolysis extracts below boiling temperature at it.(2) extraction and fractionation unites two into one.As the CO that is full of solute
2When-SF flows through separator, because pressure drop makes CO
2Become rapidly biphase (gas-liquid separation) with extract and separate immediately, not only extraction efficiency is high but also energy consumption is less, saves cost.(3) pressure and temperature can become the parameter of regulating extraction process.Near The Critical Point, the minor variations of temperature, pressure all can cause CO
2The density significant change makes the dissolubility of thing to be extracted change, and reaches the extraction purpose by controlling temperature or pressure.(4) due to overall process without organic solvent, so extract noresidue solvent prevents to the murder by poisoning of human body with to the pollution of environment.The extraction fluid can be recycled, and really realizes the production process greenization.(5) by reasonable combination of the present invention, clinical efficacy is significantly improved.(6) extract of the present invention has been removed a large amount of impurity of crude extract or irrelevant composition, and dosage is little, is easilier accepted by patient, not only can prepare all kinds of new form of administration, has simplified preparation process, and has made quality more easily control.
Checking proves through the preclinical pharmacology toxicity in the present invention: 1. maximum tolerated dose is 416.5g/kg, is equivalent to 573 times of clinical dosage.2. the long term toxicity test of pharmaceutical preparation of the present invention, arrange high 6.0g crude drug/kg, middle 3.0g crude drug/kg, low three dosage groups of 1.5g crude drug/kg, and matched group waits the capacity normal saline, and the test period is 90 days, oral administration gavage.After administration as a result 90 days and drug withdrawal two are all, each rat behavior is movable, hair is all normal.The food-intake no significant difference.The administration group rat heart, liver, spleen, lung, kidney, thyroid, adrenal gland, testis, brain, etc. organ coefficient and matched group relatively, be showed no obvious change.Hematological indices: all within range of normal value.The rat clotting time is had no significant effect.And all rats have been carried out system's postmortem, all animals internal organs are all found without abnormal.Histological examination: each system organization 26S Proteasome Structure and Function all changes without the regression after poisoning, downright bad inflammatory reaction or paraplasm.
Drug efficacy study is studied Pharmaceutical composition of the present invention according to the clinical symptoms of gastrointestinal dysfunction.Result shows that the present composition can be charged into by obvious antagonism intestinal function, improves intestinal function and suppresses, and plays the intestinal function effect of regulating; The spleen-deficient mice there is rehabilitation effect, enhancing human body immunity.The above results suggest present composition has remarkable improvement effect to gastrointestinal dysfunction or irritable bowel syndrome.Studies have shown that simultaneously: present composition pharmacological effect obviously is better than carbon dioxide supercritical fluid extraction thing of " decoction of four noble drugs " full side (after four kinds of medical materials are mixed in proportion, carry out in the lump carbon dioxide supercritical fluid extraction), significance statistical significance (P<0.01) is all arranged; Present composition pharmacological effect obviously is better than traditional volatile oil component of using+water extracting component combination or volatile oil component+ethanol extraction composition combination, and significance statistical significance (P<0.01) is all arranged.
The present invention adopts modern extraction and separation technology, and fully with extracts active ingredients out, Pharmaceutical composition of the present invention is estimated without remarkable toxic and side effects through animal acute toxicity and long term toxicity, illustrates that clinical use is safe.
Below further set forth the present invention by pharmacodynamic experiment.
Experiment one, to the regulating action of mouse small intestine motion
Sample preparation methods
Group 1, extract of the present invention: the Rhizoma Atractylodis Macrocephalae, Poria, Radix Ginseng and Radix Glycyrrhizae are ground into respectively coarse powder, adopt respectively the carbon dioxide supercritical fluid extraction device to extract, Rhizoma Atractylodis Macrocephalae coarse powder extracting pressure 32MPa, 41 ℃ of extraction temperature, extraction time 90min; Poria coarse powder extracting pressure 24MPa, 30 ℃ of extraction temperature, extraction time 170min; Radix Ginseng coarse powder extracting pressure 29MPa, 47 ℃ of extraction temperature, extraction time 180min; Radix Glycyrrhizae coarse powder extracting pressure 25MPa, 53 ℃ of extraction temperature, extraction time 200min.Obtain respectively Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract; With each extract in 1: 1: 1: 1 ratio is mixed, and get final product.
Group 2, volatile oil component+water extracting component: 1 part, 1 part of the Rhizoma Atractylodis Macrocephalae, 1 part, Poria, 1 part of Radix Ginseng and Radix Glycyrrhizae is ground into respectively coarse powder, adopts supercritical extraction to extract Rhizoma Atractylodis Macrocephalae volatile oil.Rhizoma Atractylodis Macrocephalae coarse powder extracting pressure 32MPa, 41 ℃ of extraction temperature, extraction time 90min, medicinal residues and other medical material merge, and decocting boils 3 times, and each 1 hour, collecting decoction obtained containing the water extracting component of crude drug 1g/ml after concentrated.Volatile oil is fully mixed with water extracting component, standby.
Group 3, volatile oil+alcohol extraction composition: 1 part, 1 part of the Rhizoma Atractylodis Macrocephalae, 1 part, Poria, 1 part of Radix Ginseng and Radix Glycyrrhizae is ground into respectively coarse powder, adopts supercritical extraction to extract Rhizoma Atractylodis Macrocephalae volatile oil.Rhizoma Atractylodis Macrocephalae coarse powder extracting pressure 32MPa, 41 ℃ of extraction temperature, extraction time 90min, medicinal residues and other medical material merge, 95% alcohol reflux 3 times, each 1 hour, merge extractive liquid, obtained containing the alcohol extraction composition of crude drug 1g/ml after concentrated.Volatile oil is fully mixed with the alcohol extraction composition, standby.
Group 4,1 part, 1 part of the Rhizoma Atractylodis Macrocephalae, 1 part, Poria, 1 part of Radix Ginseng and Radix Glycyrrhizae is ground into respectively coarse powder, merges and carry out carbon dioxide supercritical fluid extraction and extract, extracting pressure 32MPa, 50 ℃ of extraction temperature, extraction time 200min obtains extract, and is standby.
Group 5, Rhizoma Atractylodis Macrocephalae extract: 1 part of the Rhizoma Atractylodis Macrocephalae is ground into coarse powder, adopts supercritical extraction to obtain Rhizoma Atractylodis Macrocephalae extract.Rhizoma Atractylodis Macrocephalae powder becomes coarse powder, extracting pressure 32MPa, 41 ℃ of extraction temperature, extraction time 90min.
As requested, each group sample is made into debita spissitudo with distilled water on demand, standby.
Experimental technique
1. animal and grouping: Kunming mouse, body weight 18~22g, random packet, every group of 10 raisings.
2. experimental technique: (1) Normal group: the capacity distilled water such as gavage; (2) model group: every day the capacity distilled water such as gavage; (3) positive group: cerekinon 100mg/kg; (4) sample sets: gavage respectively test sample, by the dosed administration that is equivalent to 3.0g crude drug/kg.Each is organized gavage and gives corresponding medicine, continuously 7d.After the last administration, except Normal group, the neostigmine load is respectively organized the equal subcutaneous injection neostigmine of mice 1mg/kg; The atropine load is respectively organized the equal subcutaneous injection atropine of mice 5mg/kg.After 10min, the Azo-Blue solution 0.1ml/10g of the equal gavage 5% of all mices puts to death after 15min, cuts open the belly rapidly and takes out the gastrointestinal section, records the Azo-Blue forward position to the distance of pylorus, calculates the percentage ratio that it accounts for the small intestinal total length.A t check will be organized between each administration group.
3. experimental result shows: compare with model group, present composition group can obviously suppress the mouse small intestine hyperfunctioning (P<0.05) that the neostigmine load causes, improve the suppressed state of mouse small intestine function (P<0.05) that atropine causes, point out it to intestinal function, dual regulation to be arranged.And medicine group of the present invention (sample 1) amount group significantly is better than and other group sample (sample 2, sample 3, sample 4, sample 5) corresponding dosage group (P<0.05), the results are shown in Table 1.
The impact that table 1 advances neostigmine load/atropine load mouse small intestine, n=12
Compare with matched group: △ P<0.05.Compare with model group:
*P<0.05.Compare with 1 group, sample:
Experiment two, Radix Et Rhizoma Rhei is caused the impact of mice with spleen deficiency
1. animal and grouping: male mice in kunming, body weight 18~22g, random packet, every group of 10 raisings.
2. administration and experimental technique: except Normal group, all the other are respectively organized equal gavage and give 200% Radix Et Rhizoma Rhei decocting liquid, 0.25ml/10g, and 10d, copy the Mice with Spleen model continuously.Self-supporting Radix Et Rhizoma Rhei decocting liquid rose on the 5th day, Normal group: give the respective volume distilled water; Positive group: Ginseng Bolus for tonifying spleen 2.0g/kg; Each sample sets: be equivalent to 3.0g crude drug/kg.Each carries out spleen index analysis and freezing experiment after organizing continuous gastric infusion 6d.The results are shown in Table 2.
After mice gives Radix Et Rhizoma Rhei extract, under freezing conditions its mortality rate with give medicine mouse death rate of the present invention obvious difference (P<0.05) arranged, medicine of the present invention can obviously reduce the death toll of mice, the spleen index of rising mice illustrates that medicine of the present invention insufficiency of the spleenly has certain rehabilitation effect (P<0.05) to what Radix Et Rhizoma Rhei caused.And medicine group of the present invention (sample 1) significantly is better than and other sample sets (sample 2, sample 3, sample 4, sample 5) corresponding dosage group (P<0.05).
Table 2 medicine of the present invention causes the impact of mice with spleen deficiency on Radix Et Rhizoma Rhei
The specific embodiment
Embodiment 1
The Rhizoma Atractylodis Macrocephalae, Poria, Radix Ginseng and Chinese medicine of licorice root medical material are ground into respectively coarse powder, adopt the carbon dioxide supercritical fluid extraction device to extract.Coarse powder is packed in extractor, sets extracting pressure 29MPa, and 38 ℃ of extraction temperature are pressed CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, be extracted to without extract and produce, collect the united extraction thing from separator, obtain respectively Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract;
Get respectively Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract, by 1: 1: 1: 1 part by weight mixed, and namely obtains the present composition.Add suitable adjuvant to prepare tablet.
Embodiment 2
Rhizoma Atractylodis Macrocephalae coarse powder is packed in extractor, sets extracting pressure 33MPa, and 43 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 80min.Collect Rhizoma Atractylodis Macrocephalae extract from separator.
The Poria coarse powder is packed in extractor, sets extracting pressure 24MPa, and 31 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 160min.Collect Poria extract from separator.
The Radix Ginseng coarse powder is packed in extractor, sets extracting pressure 27MPa, and 46 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 200min.Collector's conopsea extraction from separator.
The Radix Glycyrrhizae coarse powder is packed in extractor, sets extracting pressure 25MPa, and 52 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 220min.Collect Radix Glycyrrhizae extract from separator.
Get respectively Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract, by 3: 2: 1: 2 part by weight mixed, and namely obtains the present composition.Add suitable adjuvant to prepare capsule.
Embodiment 3
Rhizoma Atractylodis Macrocephalae coarse powder is packed in extractor, sets extracting pressure 31MPa, and 40 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 90min.Collect Rhizoma Atractylodis Macrocephalae extract from separator.
The Poria coarse powder is packed in extractor, sets extracting pressure 22MPa, and 28 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 160min.Collect Poria extract from separator.
The Radix Ginseng coarse powder is packed in extractor, sets extracting pressure 30MPa, and 48 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 200min.Collector's conopsea extraction from separator.
The Radix Glycyrrhizae coarse powder is packed in extractor, sets extracting pressure 27MPa, and 54 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 200min.Collect Radix Glycyrrhizae extract from separator.
Each extract was pressed 3: 3: 2: 1 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare capsule.
Embodiment 4.
Rhizoma Atractylodis Macrocephalae coarse powder is packed in extractor, sets extracting pressure 32MPa, and 41 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 90min.Collect Rhizoma Atractylodis Macrocephalae extract from separator.
The Poria coarse powder is packed in extractor, sets extracting pressure 24MPa, and 30 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 170min.Collect Poria extract from separator.
The Radix Ginseng coarse powder is packed in extractor, sets extracting pressure 29MPa, and 47 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 180min.Collector's conopsea extraction from separator.
The Radix Glycyrrhizae coarse powder is packed in extractor, sets extracting pressure 25MPa, and 53 ℃ of extraction temperature adopt the carbon dioxide supercritical fluid extraction device to extract.Press CO
2The flow process of gas cylinder → cooling system → high-pressure pump → extractor → separator I → separator II → circulation is carried out supercritical extraction, extraction 200min.Collect Radix Glycyrrhizae extract from separator.
Each extract was pressed 1: 1: 1: 1 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare granule.
Embodiment 5
Identical with embodiment 1 method, but the each several part extract was pressed 3: 3: 2: 1 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare aerosol.
Embodiment 6. is identical with embodiment 2 methods, but the each several part extract was pressed 2: 3: 2: 1 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare gel.
Embodiment 7. is identical with embodiment 2 methods, but the each several part extract was pressed 1: 3: 1: 2 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare cataplasma.
Embodiment 8. is identical with embodiment 2 methods, but the each several part extract was pressed 1: 1: 3: 2 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare injection.
Embodiment 9. is identical with embodiment 2 methods, but the each several part extract was pressed 2: 1: 1: 3 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare granule.
Embodiment 10. is identical with embodiment 2 methods, but the each several part extract was pressed 2: 2: 1: 2 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare pellet.
Embodiment 11. is identical with embodiment 1 method, but the each several part extract was pressed 1: 2: 2: 2 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare inhalant.
Embodiment 12. is identical with embodiment 1 method, but the each several part extract was pressed 3: 1: 1: 2 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare Emulsion.
Embodiment 13. is identical with embodiment 1 method, but the each several part extract was pressed 3: 1: 1: 2 part by weight mixes, and namely obtains the present composition.Add suitable adjuvant to prepare drop pill.
Claims (7)
1. compositions for the treatment of the Chinese medicine extract of gastrointestinal dysfunction, it is characterized in that said composition is composed of the following components: Rhizoma Atractylodis Macrocephalae extract 1 ~ 3 weight portion, Poria extract 1 ~ 3 weight portion, Radix Ginseng extract 1 ~ 2 weight portion, Radix Glycyrrhizae extract 1 ~ 2 weight portion; Described extract adopts carbon dioxide supercritical extraction method to extract for Chinese medicinal material is ground into coarse powder, and said composition specifically prepares by following steps:
The Rhizoma Atractylodis Macrocephalae, Poria, Radix Ginseng and Radix Glycyrrhizae are ground into respectively coarse powder, adopt respectively the carbon dioxide supercritical fluid extraction device to extract, extracting pressure 25~34MPa, 28~54 ℃ of extraction temperature, be extracted to without the extract generation, obtain Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract; Each extract is mixed in proportion.
2. the compositions of the Chinese medicine extract for the treatment of gastrointestinal dysfunction according to claim 1, it is characterized in that said composition is composed of the following components: Rhizoma Atractylodis Macrocephalae extract 1 weight portion, Poria extract 1 weight portion, Radix Ginseng extract 1 weight portion, Radix Glycyrrhizae extract 1 weight portion; Described extract adopts carbon dioxide supercritical extraction method to extract for Chinese medicinal material is ground into coarse powder.
3. the compositions of the Chinese medicine extract for the treatment of gastrointestinal dysfunction according to claim 1 is characterized in that what said composition specifically prepared by following steps:
(1) Rhizoma Atractylodis Macrocephalae is ground into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 31~34 MPa, and 40~43 ℃ of extraction temperature, extraction time 80-90 min collects Rhizoma Atractylodis Macrocephalae extract;
(2) Poria powder is broken into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 22~25 MPa, and 28~32 ℃ of extraction temperature, extraction time 160-180 min collects Poria extract;
(3) Radix Ginseng powder is broken into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 26~30 MPa, 45~48 ℃ of extraction temperature, extraction time 180-200 min, collector's conopsea extraction;
(4) Radix Glycyrrhizae powder is broken into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 24~27 MPa, and 51~54 ℃ of extraction temperature, extraction time 200-220 min collects Radix Glycyrrhizae extract;
(5) each extract is mixed in proportion, and get final product.
4. the compositions of the Chinese medicine extract for the treatment of gastrointestinal dysfunction according to claim 1 is characterized in that adding corresponding adjuvant be prepared into oral formulations, mucocutaneous drug-delivery preparation or suck preparation in the compositions of this Chinese medicine extract.
5. the preparation method of the compositions of the Chinese medicine extract of a treatment gastrointestinal dysfunction claimed in claim 1 is characterized in that the method comprises the following steps:
The Rhizoma Atractylodis Macrocephalae, Poria, Radix Ginseng and Radix Glycyrrhizae are ground into respectively coarse powder, adopt respectively the carbon dioxide supercritical fluid extraction device to extract, extracting pressure 25~34MPa, 28~54 ℃ of extraction temperature, be extracted to without the extract generation, obtain Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Ginseng extract and Radix Glycyrrhizae extract; Each extract is mixed in proportion.
6. the preparation method of the compositions of the Chinese medicine extract for the treatment of gastrointestinal dysfunction according to claim 5 is characterized in that the method comprises the following steps:
(1) Rhizoma Atractylodis Macrocephalae is ground into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 31~34 MPa, and 40~43 ℃ of extraction temperature, extraction time 80-90 min collects Rhizoma Atractylodis Macrocephalae extract;
(2) Poria powder is broken into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 22~25 MPa, and 28~32 ℃ of extraction temperature, extraction time 160-180 min collects Poria extract;
(3) Radix Ginseng powder is broken into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 26~30 MPa, 45~48 ℃ of extraction temperature, extraction time 180-200 min, collector's conopsea extraction;
(4) Radix Glycyrrhizae powder is broken into coarse powder, adopts carbon dioxide supercritical fluid extraction, extracting pressure 24~27 MPa, and 51~54 ℃ of extraction temperature, extraction time 200-220 min collects Radix Glycyrrhizae extract;
(5) each extract is mixed in proportion.
7. the application of compositions claimed in claim 1 in the medicine of preparation treatment gastrointestinal dysfunction.
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李大鹏等.超临界二氧化碳萃取中药有效成分应用进展.《现代中药制剂发展与中药药理学研究交流会论文集》.2009,第290页"1 超临界二氧化氮萃取中药有效成分的优势"项下,第291页第13-14行,37-42行,第292页第2-6行. * |
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