CN102246914A - 利用稀酸浸提蛋白质类食品中重金属的方法与应用 - Google Patents
利用稀酸浸提蛋白质类食品中重金属的方法与应用 Download PDFInfo
- Publication number
- CN102246914A CN102246914A CN201110189397XA CN201110189397A CN102246914A CN 102246914 A CN102246914 A CN 102246914A CN 201110189397X A CN201110189397X A CN 201110189397XA CN 201110189397 A CN201110189397 A CN 201110189397A CN 102246914 A CN102246914 A CN 102246914A
- Authority
- CN
- China
- Prior art keywords
- heavy metal
- protein
- diluted acid
- based food
- lixiviate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 112
- 238000000034 method Methods 0.000 title claims abstract description 69
- 239000002253 acid Substances 0.000 title claims abstract description 52
- 235000013305 food Nutrition 0.000 title claims abstract description 38
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 35
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 35
- 238000002386 leaching Methods 0.000 title abstract description 7
- 238000005516 engineering process Methods 0.000 claims abstract description 11
- 239000000126 substance Substances 0.000 claims abstract description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 20
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 18
- 241000272525 Anas platyrhynchos Species 0.000 claims description 8
- 241000238557 Decapoda Species 0.000 claims description 8
- 241000287828 Gallus gallus Species 0.000 claims description 6
- 238000002203 pretreatment Methods 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 238000012271 agricultural production Methods 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 2
- 241000272814 Anser sp. Species 0.000 claims description 2
- 241000237502 Ostreidae Species 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 241000282898 Sus scrofa Species 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 235000020636 oyster Nutrition 0.000 claims description 2
- 235000015170 shellfish Nutrition 0.000 claims description 2
- 239000010949 copper Substances 0.000 abstract description 41
- 229910052793 cadmium Inorganic materials 0.000 abstract description 40
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 abstract description 40
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 abstract description 39
- 229910052802 copper Inorganic materials 0.000 abstract description 39
- 238000002965 ELISA Methods 0.000 abstract description 32
- 238000006243 chemical reaction Methods 0.000 abstract description 15
- 238000001514 detection method Methods 0.000 abstract description 15
- 150000002500 ions Chemical class 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 2
- 244000144972 livestock Species 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 56
- 239000000243 solution Substances 0.000 description 37
- 239000000047 product Substances 0.000 description 20
- 238000010561 standard procedure Methods 0.000 description 20
- 238000012360 testing method Methods 0.000 description 20
- 238000011084 recovery Methods 0.000 description 16
- 239000006228 supernatant Substances 0.000 description 14
- 238000010183 spectrum analysis Methods 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 241001522252 Crassostrea rivularis Species 0.000 description 12
- 238000005303 weighing Methods 0.000 description 12
- 229910052751 metal Inorganic materials 0.000 description 11
- 239000002184 metal Substances 0.000 description 11
- 238000002360 preparation method Methods 0.000 description 11
- 241001245601 Perna viridis Species 0.000 description 9
- 238000000605 extraction Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 239000013535 sea water Substances 0.000 description 8
- 238000000120 microwave digestion Methods 0.000 description 7
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 235000013330 chicken meat Nutrition 0.000 description 5
- 229910001431 copper ion Inorganic materials 0.000 description 5
- 235000015277 pork Nutrition 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 235000015278 beef Nutrition 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000012417 linear regression Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000012224 working solution Substances 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 2
- 229960001484 edetic acid Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910017604 nitric acid Inorganic materials 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 210000004872 soft tissue Anatomy 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 description 1
- 238000004380 ashing Methods 0.000 description 1
- 238000003321 atomic absorption spectrophotometry Methods 0.000 description 1
- 238000001391 atomic fluorescence spectroscopy Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- -1 diethyl pentetic acid Chemical compound 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000010791 domestic waste Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000002354 inductively-coupled plasma atomic emission spectroscopy Methods 0.000 description 1
- 239000011133 lead Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229960003330 pentetic acid Drugs 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000000918 plasma mass spectrometry Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000000209 wet digestion Methods 0.000 description 1
Images
Landscapes
- Processing Of Solid Wastes (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110189397XA CN102246914B (zh) | 2011-07-07 | 2011-07-07 | 利用稀酸浸提蛋白质类食品中重金属的方法与应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110189397XA CN102246914B (zh) | 2011-07-07 | 2011-07-07 | 利用稀酸浸提蛋白质类食品中重金属的方法与应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102246914A true CN102246914A (zh) | 2011-11-23 |
CN102246914B CN102246914B (zh) | 2013-01-02 |
Family
ID=44974776
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201110189397XA Expired - Fee Related CN102246914B (zh) | 2011-07-07 | 2011-07-07 | 利用稀酸浸提蛋白质类食品中重金属的方法与应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102246914B (zh) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102539742A (zh) * | 2011-12-13 | 2012-07-04 | 暨南大学 | 一种检测碳水化合物类农产品中重金属的方法与应用 |
CN103575583A (zh) * | 2013-11-21 | 2014-02-12 | 国家粮食局科学研究院 | 一种从谷物中快速温和提取重金属的方法 |
CN104181024A (zh) * | 2014-09-10 | 2014-12-03 | 江苏天瑞仪器股份有限公司 | 一种用于谷物类农作物中镉元素快速测定的萃取液 |
CN104749364A (zh) * | 2013-12-25 | 2015-07-01 | 深圳先进技术研究院 | 一种检测锌的免疫分析方法及检测锌的免疫分析检测试剂盒 |
CN105452841A (zh) * | 2013-05-24 | 2016-03-30 | 株式会社住化分析中心 | 用于测定农作物试样中的有害元素量的试样的前处理方法 |
CN106018713A (zh) * | 2016-05-03 | 2016-10-12 | 成都亿信标准认证集团有限公司 | 食物重金属自动检测系统 |
CN107702972A (zh) * | 2017-09-07 | 2018-02-16 | 暨南大学 | 二氧化钛在酶联免疫法检测土壤中重金属离子的前处理中的应用 |
CN107727645A (zh) * | 2017-09-07 | 2018-02-23 | 暨南大学 | 多聚磷酸铝在酶联免疫法检测土壤中重金属离子的前处理中的应用 |
CN107966348A (zh) * | 2017-11-23 | 2018-04-27 | 中国农业科学院农产品加工研究所 | 一种青稞中重金属元素检测的快速前处理方法 |
CN108036983A (zh) * | 2017-12-08 | 2018-05-15 | 微粒云科技(北京)有限公司 | 一种含有蛋白质的样本中金属离子的检测方法及仪器包 |
CN110411812A (zh) * | 2019-09-06 | 2019-11-05 | 国家粮食和物资储备局科学研究院 | 一种羧基磁珠用于富集净化粮食中重金属的应用 |
CN112881376A (zh) * | 2021-01-15 | 2021-06-01 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | 一种水产品重金属镉残留量快速检测的方法 |
CN113142464A (zh) * | 2021-03-22 | 2021-07-23 | 陈元曦 | 海产品重金属去除方法 |
-
2011
- 2011-07-07 CN CN201110189397XA patent/CN102246914B/zh not_active Expired - Fee Related
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102539742A (zh) * | 2011-12-13 | 2012-07-04 | 暨南大学 | 一种检测碳水化合物类农产品中重金属的方法与应用 |
CN102539742B (zh) * | 2011-12-13 | 2014-12-03 | 暨南大学 | 一种检测碳水化合物类农产品中重金属的方法与应用 |
CN105452841A (zh) * | 2013-05-24 | 2016-03-30 | 株式会社住化分析中心 | 用于测定农作物试样中的有害元素量的试样的前处理方法 |
CN103575583A (zh) * | 2013-11-21 | 2014-02-12 | 国家粮食局科学研究院 | 一种从谷物中快速温和提取重金属的方法 |
CN103575583B (zh) * | 2013-11-21 | 2016-04-20 | 国家粮食局科学研究院 | 一种从谷物中快速温和提取重金属的方法 |
CN104749364A (zh) * | 2013-12-25 | 2015-07-01 | 深圳先进技术研究院 | 一种检测锌的免疫分析方法及检测锌的免疫分析检测试剂盒 |
CN104181024A (zh) * | 2014-09-10 | 2014-12-03 | 江苏天瑞仪器股份有限公司 | 一种用于谷物类农作物中镉元素快速测定的萃取液 |
CN106018713A (zh) * | 2016-05-03 | 2016-10-12 | 成都亿信标准认证集团有限公司 | 食物重金属自动检测系统 |
CN107702972A (zh) * | 2017-09-07 | 2018-02-16 | 暨南大学 | 二氧化钛在酶联免疫法检测土壤中重金属离子的前处理中的应用 |
CN107727645A (zh) * | 2017-09-07 | 2018-02-23 | 暨南大学 | 多聚磷酸铝在酶联免疫法检测土壤中重金属离子的前处理中的应用 |
CN107966348A (zh) * | 2017-11-23 | 2018-04-27 | 中国农业科学院农产品加工研究所 | 一种青稞中重金属元素检测的快速前处理方法 |
CN108036983A (zh) * | 2017-12-08 | 2018-05-15 | 微粒云科技(北京)有限公司 | 一种含有蛋白质的样本中金属离子的检测方法及仪器包 |
CN108036983B (zh) * | 2017-12-08 | 2021-07-13 | 微粒云科技(北京)有限公司 | 一种含有蛋白质的样本中金属离子的检测方法及仪器包 |
CN110411812A (zh) * | 2019-09-06 | 2019-11-05 | 国家粮食和物资储备局科学研究院 | 一种羧基磁珠用于富集净化粮食中重金属的应用 |
CN112881376A (zh) * | 2021-01-15 | 2021-06-01 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | 一种水产品重金属镉残留量快速检测的方法 |
CN113142464A (zh) * | 2021-03-22 | 2021-07-23 | 陈元曦 | 海产品重金属去除方法 |
Also Published As
Publication number | Publication date |
---|---|
CN102246914B (zh) | 2013-01-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102246914B (zh) | 利用稀酸浸提蛋白质类食品中重金属的方法与应用 | |
Le et al. | Human urinary arsenic excretion after one-time ingestion of seaweed, crab, and shrimp | |
EFSA Panel on Contaminants in the Food Chain (CONTAM) | Scientific Opinion on arsenic in food | |
Laird et al. | Bioaccessibility of metals in fish, shellfish, wild game, and seaweed harvested in British Columbia, Canada | |
Shah et al. | Total mercury determination in different tissues of broiler chicken by using cloud point extraction and cold vapor atomic absorption spectrometry | |
Bashir et al. | Evaluation of trace metal levels in tissues of two commercial fish species in Kapar and Mersing coastal waters, Peninsular Malaysia | |
CN102539742B (zh) | 一种检测碳水化合物类农产品中重金属的方法与应用 | |
Leichliter et al. | Nitrogen isotopes in tooth enamel record diet and trophic level enrichment: Results from a controlled feeding experiment | |
Perga | Taphonomic and early diagenetic effects on the C and N stable isotope composition of cladoceran remains: implications for paleoecological studies | |
Kirby et al. | Arsenic concentrations and speciation in a temperate mangrove ecosystem, NSW, Australia | |
Sun et al. | Bioaccessibility of cadmium in fresh and cooked Agaricus blazei Murill assessed by in vitro biomimetic digestion system | |
Quiroz et al. | Binding of thorium (IV) to carboxylate, phosphate and sulfate functional groups from marine exopolymeric substances (EPS) | |
CN102928500A (zh) | 微波消解-icp-ms法直接测定海产品中有机硒、蛋白硒或多糖硒的检测方法 | |
Grasso et al. | Dietary exposure of zinc oxide nanoparticles (ZnO-NPs) from canned seafood by single particle ICP-MS: Balancing of risks and benefits for human health | |
Qiu et al. | Species distribution characteristics of arsenic in shellfish seafood collected from Fujian Province of China | |
Kuehnelt et al. | Organoarsenic compounds in the terrestrial environment | |
Kwaśniak et al. | The assessment of organic mercury in Baltic fish by use of an in vitro digestion model | |
García-Llatas et al. | 11 minerals and trace elements | |
Chen et al. | Microcystin accumulation in freshwater bivalves from Lake Taihu, China, and the potential risk to human consumption | |
CN105768086A (zh) | 一种食用菌蛋白肽-硒螯合物的制备方法 | |
CN109709322B (zh) | 一种检测黄曲霉毒素b1的检测方法 | |
De Bettencourt et al. | Refractory arsenic species in estuarine waters | |
CN102608085A (zh) | 一种检测藻类溶血毒素活性的方法与应用 | |
Planer-Friedrich et al. | Dimethylated thioarsenates: a potentially dangerous blind spot in current worldwide regulatory limits for arsenic in rice | |
Jørgensen et al. | Relation between the concentration of Dinophysis acuminata and diarrheic shellfish poisoning toxins in blue mussels (Mytilus edulis) during a toxic episode in the Limfjord (Denmark), 2006 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20111123 Assignee: SHENZHEN CITY TRIPHIL BIO-TECH Co.,Ltd. Assignor: Jinan University Contract record no.: 2015440020046 Denomination of invention: Method for leaching heavy metals in protein foods by using diluted acid and application thereof Granted publication date: 20130102 License type: Exclusive License Record date: 20150211 |
|
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130102 |