CN102240067A - Method for preparing fructus fosae laevigatae extracts, application thereof in cigarette and cigarette - Google Patents
Method for preparing fructus fosae laevigatae extracts, application thereof in cigarette and cigarette Download PDFInfo
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Abstract
The invention discloses a method for preparing fructus fosae laevigatae extracts. The fructus fosae laevigatae is extracted by adopting a method combining alcohol and water, so that the effective components in the fructus fosae laevigatae can be extracted more completely; and meanwhile, gel column chromatographic separation is selected in separation, and the specific adsorption action of the polyamide is utilized, so that macromolecule substances, such as tannin and the like which can affect the effects of tobacco adding fragrances can be removed, and the content of the effective components in the fructus fosae laevigatae extract can be ensured. By utilizing the extraction method of the fructus fosae laevigatae, on the one hand, the purification effect can be improved in the production process, and the product quality of the extract product can be improved, thus the whole production process is efficient, and is suitable for industrial implementation; and on the other hand, the fructus fosae laevigatae extract obtained by the preparation method provided by the invention is applied to the preparation of the cigarette, is convenient in use, and can be used for greatly improving the intrinsic quality of the cigarette, thus the stimulation of the cigarette is reduced, the smoke gas is increased, the sweet smoothness of the smoke gas is improved, and the intrinsic quality of the cigarette is greatly improved.
Description
Technical field
The present invention relates to plant extract and application thereof, the cigarette that particularly relates to a kind of preparation method of Fructus Rosae Laevigatae extract and the application in cigarette thereof and comprise this Fructus Rosae Laevigatae extract.
Background technology
The fruit of Cherokee rose (Rosa laevigata Mickx) has another name called sugared orange, honeybee sugar bowl, thorn pear, Randia spinosa etc., belongs to rosaceous plant.The fruit of Cherokee rose is a conventional Chinese medicine, and medicinal long, determined curative effect is reliable.Compendium of Material Medica record: fruit of Cherokee rose property acid, puckery, flat, nontoxic; Return kidney, bladder, large intestine channel.
For the extraction of the fruit of Cherokee rose, in the traditional handicraft, have to relate to and adopt ethanol that the fruit of Cherokee rose is extracted, utilize resin to carry out the method that concentrate drying after the separation and purification obtains extract.But the Fructus Rosae Laevigatae extract active constituent content that this method obtains is low, is unsuitable for industrializing implementation.And the Fructus Rosae Laevigatae extract that obtains in the traditional handicraft is dried powder, also is unsuitable for the long period preservation.How extraction process being improved, with the Fructus Rosae Laevigatae extract content of effective that raising obtains, is the direction that people make great efforts.On the other hand, present stage, because the pharmacological action of the fruit of Cherokee rose, the application study of the domestic relevant fruit of Cherokee rose mainly concentrates on the medicines and health protection field, and is less in the application of other field, and relevant report is not seen in the application in cigarette.
Summary of the invention
Technical problem to be solved by this invention is: remedy above-mentioned the deficiencies in the prior art, propose a kind of preparation method of Fructus Rosae Laevigatae extract, can improve content of effective in the Fructus Rosae Laevigatae extract that obtains by a relatively large margin.
Another technical problem to be solved by this invention is: Fructus Rosae Laevigatae extract is applied to can greatly improve the interior quality of cigarette in the cigarette manufacturing.
Technical problem of the present invention is solved by following technical scheme:
A kind of preparation method of Fructus Rosae Laevigatae extract may further comprise the steps: 1) pulverize: fruit of Cherokee rose raw material to be extracted is dried under 40～70 ℃ of conditions, and be crushed to the fruit of Cherokee rose powder that granularity is 150～420um; 2) alcohol extracting: it is that the mass fraction of 8～15 times of described fruit of Cherokee rose powder qualities is in 50%～80% the ethanolic solution that described fruit of Cherokee rose powder is added quality, refluxing extraction obtains extract, use the described extract of 150～200 purpose screen filtrations to obtain filtrate, described filtrate decompression is concentrated, get the alcohol extract and the dregs of a decoction; 3) water extracts: with adding quality in the described dregs of a decoction after the alcohol extracting is the water of 8～15 times of described dregs of a decoction quality, the backflow lixiviate obtains extract, use the described extract of 150～200 purpose screen filtrations to obtain filtrate, described filtrate decompression concentrate concentrate, add ethanol and make and contain the alcohol amount in the described concentrate and reach 50～75%, stir static 8～24h below 4 ℃, described concentrate filtered obtains filtrate, with described filtrate decompression concentrate aqueous extract; 4) aqueous extract that obtains in alcohol extract that obtains polyamide gels column chromatography separation: with step 2) and the step 3) mixes, obtain total extract, the mass fraction that adds quality and be 4～6 times of described total extract quality in described total extract is that 70～95% ethanolic solution dissolves, obtain lysate, with polyamide gels post on the described lysate, water and/or alcohol flushing are collected eluent; 5) concentrate: it is concentrated that the eluent that obtains in the step 4) is reduced pressure, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.
In the optimized technical scheme,
The consumption of the polyamide gels in the post of polyamide gels described in the step 4) is 1～5 times of described total extract quality, and granularity is 60～80 orders, the blade diameter length ratio 1:4～1:10 of described polyamide gels column chromatography, and the absorption flow velocity is 0.5～4.0BV/h.
Also comprise the film separating step in the step 4): is that the daltonian hollow-fibre membrane of 3k～10k separates with described eluent by molecular weight, collects to see through liquid; Described step 5) reduces pressure concentratedly for the described liquid that sees through that will obtain, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.Like this,, can further selectively remove some macromolecular substances, improve and extract the fruit of Cherokee rose content of effective that obtains by increasing the film separating step.
Also comprise centrifugation step in the step 4): described lysate centrifugation is gone out supernatant,, collect eluent behind water and/or the alcohol flushing with polyamide gels post on the described supernatant.By before the polyamide gels column chromatography, centrifugation step being set, make total extract have higher clarity and stability, can avoid when last polyamide gels column chromatography, stopping up filler, reduce the situation of chromatography efficient.
Centrifugation rate is 2000～8000rpm during described centrifugation, and the time is 10～30min.
Step 2) refluxing extraction obtains extract and is specially in: refluxing extraction 1～2 time, and each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract; The backflow lixiviate obtains extract and is specially in the step 3): backflow lixiviate 1～2 time, and each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract.
When decompression concentrated step 2), relative density was 1.12～1.16 to obtain the described alcohol extract and the dregs of a decoction when described filtrate decompression was concentrated into 25 ℃; When decompression concentrates in the step 3), when described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 concentrate; When described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 aqueous extract; When decompression concentrated in the step 5), relative density was 1.20～1.40 when described eluent was evaporated to 25 ℃, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.Concentrate control through this decompression, the extract product that obtains can reach certain viscosity, and still has certain fluidity, does not generally need to add anticorrisive agent under this state in the extract product and just can preserve the long period.Make that promptly the Fructus Rosae Laevigatae extract after concentrating has better stability.
Technical problem of the present invention is solved by following further technical scheme:
A kind of application of Fructus Rosae Laevigatae extract in cigarette that obtains according to above-mentioned method diluted described Fructus Rosae Laevigatae extract with the nontoxic organic solvent that has no irritating odor, extension rate is 10-15 times; Before the preparation cigarette, described Fructus Rosae Laevigatae extract dilution is sprayed at the cured tobacco leaf surface according to the mass ratio of 0.1%-1%.
Described organic solvent is propane diols, sorbierite, xylitol or glycerine, perhaps wherein any mixed liquor more than 2 kinds.
Technical problem of the present invention is solved by following further technical scheme:
A kind of cigarette is added with the Fructus Rosae Laevigatae extract that obtains according to above-mentioned method in the described cigarette.
The beneficial effect that the present invention is compared with the prior art is:
The extracting method of the fruit of Cherokee rose of the present invention, adopt alcohol, water to extract the fruit of Cherokee rose in conjunction with the method for extracting, make the active ingredient in the fruit of Cherokee rose can extract comparatively fully, select for use gel column chromatography to separate when separating simultaneously, utilize the specific adsorption effect of polyamide, can remove most of influences the macromolecular substances such as tannin of tobacco flavoring effect in the back extended meeting, guarantees content of effective in the Fructus Rosae Laevigatae extract.The extracting method of the fruit of Cherokee rose of the present invention can be improved the purification effect in the production process, improve to extract the product quality of product, makes in the whole process of production efficiently, is suitable for industrializing implementation.On the other hand, the resulting Fructus Rosae Laevigatae extract of preparation method of the present invention is applied in the cigarette preparation, easy to use, and can greatly improve the interior quality of cigarette, the cigarette excitant is descended, exhaust gas volumn increases, the sweet property raising of flue gas has greatly improved the interior quality of cigarette.
The specific embodiment
Below in conjunction with the specific embodiment the present invention is described in further details.
A kind of preparation method of Fructus Rosae Laevigatae extract may further comprise the steps:
1) pulverizes: fruit of Cherokee rose raw material to be extracted is dried under 40～70 ℃ of conditions, and be crushed to the meal that granularity is 150～420um.With raw material pulverizing is meal, makes the fruit of Cherokee rose that higher recovery rate be arranged in subsequent extracted.
2) alcohol extracting: it is that the mass fraction of 8～15 times of fruit of Cherokee rose powder qualities is in 50%～80% the ethanolic solution that fruit of Cherokee rose powder is added quality, refluxing extraction obtains extract, use 150～200 purpose screen filtration extracts to obtain filtrate, filtrate decompression is concentrated, get the alcohol extract and the dregs of a decoction.Wherein, specifically can be during refluxing extraction: refluxing extraction 1～2 time, each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract.
3) water extraction: with step 2) adding quality in the dregs of a decoction that obtain after the alcohol extracting is the water of 8～15 times of described dregs of a decoction quality, the backflow lixiviate obtains extract, use 150～200 purpose screen filtration extracts to obtain filtrate, filtrate decompression concentrate concentrate, add ethanol and make and contain the alcohol amount in the concentrate and reach 50～75%, stir, static 8～24h below 4 ℃, filtration obtains filtrate to concentrate, and filtrate decompression is concentrated, and gets aqueous extract.Wherein, the backflow lixiviate obtains extract and is specially: backflow lixiviate 1～2 time, and each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract.
Above-mentioned employing alcohol, water extract the fruit of Cherokee rose in conjunction with the method for extracting, and the composition in the fruit of Cherokee rose can be extracted comparatively fully, make extract all can obtain good effect at color and luster and follow-up filtration difficulty or ease, the final aspects such as active constituent content that obtain of extracting.
4) aqueous extract that obtains in alcohol extract that obtains polyamide gels column chromatography separation: with step 2) and the step 3) mixes, obtain total extract, the mass fraction that adds quality and be 4～6 times of total extract quality in total extract is that 70～95% ethanolic solution dissolves, get lysate, with polyamide gels post on the described lysate, water and/or alcohol flushing are collected eluent.Wherein, the consumption of the polyamide gels in the polyamide gels post is 1～5 times of total extract quality, and granularity is 60～80 orders, the blade diameter length ratio 1:4～1:10 of polyamide gels column chromatography, and the absorption flow velocity is 0.5～4.0BV/h.Through the polyamide gels column chromatography, utilize the specific adsorption effect of polyamide, can remove most of macromolecular substances such as tannin that influence the tobacco flavoring effect in the back extended meeting.
Preferably, also comprise centrifugation step in the step 4): described lysate centrifugation is gone out supernatant,, collect eluent behind water and/or the alcohol flushing with polyamide gels post on the described supernatant.Centrifugal before the polyamide gels column chromatography makes total extract have higher clarity and stability, can avoid stopping up filler when last polyamide gels column chromatography, reduces the situation of chromatography efficient.Wherein, centrifugation rate is 2000～8000rpm during centrifugation, and the time is 10～30min.
5) concentrate: it is concentrated that the eluent that obtains in the step 4) is reduced pressure, and the thick solution after concentrating is Fructus Rosae Laevigatae extract.
Preferably, also comprise the film separating step in the step 4): is that the daltonian hollow-fibre membrane of 3k～10k separates with eluent by molecular weight, collects to see through liquid.Described step 5) is the described liquid that sees through that will obtain and reduces pressure concentratedly, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.Increase the film separating step in the step 4), can further selectively remove some macromolecular substances, improve and extract the fruit of Cherokee rose content of effective that obtains.
When decompression concentrated further preferably, in the above steps, when decompression concentrates, 2), relative density was 1.12～1.16 to obtain the described alcohol extract and the dregs of a decoction when described filtrate decompression was concentrated into 25 ℃; When decompression concentrates in the step 3), when described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 concentrate; When described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 aqueous extract; When decompression concentrated in the step 5), relative density was 1.20～1.40 when described eluent was evaporated to 25 ℃, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.Concentrate control through this decompression, the extract product that obtains can reach certain viscosity, and still has certain fluidity, does not generally need to add anticorrisive agent under this state in the extract product and just can preserve the long period.Make that promptly the Fructus Rosae Laevigatae extract after concentrating has better stability.
The Fructus Rosae Laevigatae extract that obtains among the above-mentioned preparation method, adopt alcohol, water to extract the fruit of Cherokee rose in conjunction with the method for extracting, make the active ingredient in the fruit of Cherokee rose can extract comparatively fully, select for use gel column chromatography to separate when separating simultaneously, utilize the specific adsorption effect of polyamide, can remove most of influences the macromolecular substances such as tannin of tobacco flavoring effect in the back extended meeting, guarantees content of effective in the Fructus Rosae Laevigatae extract.
The Fructus Rosae Laevigatae extract that obtains according to the method described above is applied in the cigarette, at first, Fructus Rosae Laevigatae extract is diluted with the nontoxic organic solvent that has no irritating odor, extension rate is 10-15 times.Wherein, organic solvent can be propane diols, sorbierite, xylitol or glycerine, perhaps wherein any mixed liquor more than 2 kinds.Secondly, before the preparation cigarette, the Fructus Rosae Laevigatae extract dilution is sprayed at the cured tobacco leaf surface according to the mass ratio of 0.1%-1%.Then, cigarette process is routinely handled, and promptly after spraying the dilution of Fructus Rosae Laevigatae extract, with flue-cured tobacco tobacco storage 4 hours, makes cigarette finished product through chopping, baking silk, perfuming, storage silk, cigarette and packaging process again.
Embodiment 1
Accurately take by weighing the 200g fruit of Cherokee rose,, and be crushed to the meal that granularity is 250um 45 ℃ of oven dry, adding 1.6kg concentration is 60% ethanolic solution, refluxing extraction 2 times, each 1h, extract filters through 200 eye mesh screens, and relative density is 1.12 when being evaporated to 25 ℃, gets alcohol extract; The water refluxing extraction 1 time that in the dregs of a decoction, adds 2kg, extract 2h, extract filters through 200 eye mesh screens, relative density is 1.12 when being evaporated to 25 ℃, adds ethanol and makes and contain the alcohol amount and reach 65%, stirs, static 10h below 4 ℃, filter, decompression filtrate recycling ethanol and when being concentrated into 25 ℃ relative density be 1.14, aqueous extract; Merging alcohol extract and aqueous extract, add 4 times 80% ethanol dissolving, is centrifugation 20min under the 4000rpm at centrifugation rate, obtains supernatant; Supernatant dynamically adsorbs by the polyamide gels post, and water and/or alcohol flushing are collected eluent, and wherein the consumption of polyamide gels is 1.5 times of total extract quality, and granularity is 60～80 orders, and the blade diameter length ratio of chromatography is 1:8, and the absorption flow velocity is 1BV/h; Eluent is the daltonian hollow-fibre membrane of 5k through molecular cut off again, collects and sees through liquid, and relative density is 1.25 when being evaporated to 25 ℃, is the Fructus Rosae Laevigatae extract I.
Embodiment 2
Accurately take by weighing the 200g fruit of Cherokee rose,, and be crushed to the meal that granularity is 420um 50 ℃ of oven dry, adding 2.4kg concentration is 70% ethanolic solution, and refluxing extraction 1 time is extracted 3h, extract filters through 200 eye mesh screens, and relative density is 1.15 when being evaporated to 25 ℃, gets alcohol extract; The water refluxing extraction 1 time that in the dregs of a decoction, adds 2.4kg, extract 3h, extract filters through 200 eye mesh screens, relative density is 1.14 when being evaporated to 25 ℃, adds ethanol and makes and contain the alcohol amount and reach 75%, stirs, static 8h below 4 ℃, filter, decompression filtrate recycling ethanol and when being concentrated into 25 ℃ relative density be 1.13, aqueous extract; Merging alcohol extract and aqueous extract, add 3 times 95% ethanol dissolving, is centrifugation 10min under the 6000rpm at centrifugation rate, obtains supernatant; Supernatant dynamically adsorbs by polyamide gels, and water and/or alcohol flushing are collected eluent, and wherein the consumption of polyamide gels is 2 times of total extract, and granularity is 60～80 orders, and the blade diameter length ratio of chromatography is 1:4, and the absorption flow velocity is 0.5BV/h; Eluent is the daltonian hollow-fibre membrane of 3k through molecular cut off again, collects and sees through liquid, and relative density is 1.31 when being evaporated to 25 ℃, is the Fructus Rosae Laevigatae extract II.
Embodiment 3
Accurately take by weighing the 200g fruit of Cherokee rose,, and be crushed to the meal that granularity is 250um 65 ℃ of oven dry, add 1.6 kg concentration and be 50% ethanolic solution, refluxing extraction 2 times, each 2h, extract filters through 200 eye mesh screens, and relative density is 1.12 when being evaporated to 25 ℃, gets alcohol extract; The water refluxing extraction 2 times that in the dregs of a decoction, adds 1.6kg, each 1h that extracts, extract filters through 200 eye mesh screens, relative density is 1.14 when being evaporated to 25 ℃, adds ethanol and makes and contain the alcohol amount and reach 50%, stirs, static 16h below 4 ℃, filter, decompression filtrate recycling ethanol and when being concentrated into 25 ℃ relative density be 1.13, aqueous extract; Merging alcohol extract and aqueous extract, add 3 times 75% ethanol dissolving, is centrifugation 15min under the 5000rpm at centrifugation rate, obtains supernatant; Supernatant dynamically adsorbs by polyamide gels, and water and/or alcohol flushing are collected eluent, and wherein the consumption of polyamide gels is 3 times of total extract, and granularity is 60～80 orders, and the blade diameter length ratio of chromatography is 1:10, and the absorption flow velocity is 2.5BV/h; Eluent is the daltonian hollow-fibre membrane of 8k through molecular cut off again, collects and sees through liquid, and relative density is 1.33 when being evaporated to 25 ℃, is the Fructus Rosae Laevigatae extract III.
Experimental test: the Pericarpium Trichosanthis extract I, II, the III that make in the foregoing description 1,2 and 3 all are diluted to 10 times with 50% propylene glycol solution, are sprayed at then with a kind of middle-grade blank (not flavoring and casing) flue-cured tobacco pipe tobacco surface, addition is 0.5% of a pipe tobacco.Subsequent treatment cigarette process routinely carries out, and promptly after spraying the dilution of Fructus Rosae Laevigatae extract, with flue-cured tobacco tobacco storage 4 hours, makes cigarette finished product through chopping, baking silk, perfuming, storage silk, cigarette and packaging process again.To the contrast of smokeing panel test of above-mentioned sample, the overall sensory test result who obtains is as shown in the table respectively in the group of smokeing panel test that is made up of 8 people.Wherein, the interior quality of blank sample all is set at 0, improves to some extent (alleviate as excitant, exhaust gas volumn increases, and sweet sense improves) with respect to the blank sample interior quality, then mutually deservedly just divide, score value is high more, and it is good more to improve; Decrease (increase as excitant, exhaust gas volumn reduces, and sweet sense reduces) with respect to the blank sample interior quality, then mutually deserved negative branch, negative branch is low more, and it is poor more to improve.
| Sample | Excitant is improved | Exhaust gas volumn is improved | Sweet sense improves |
| Blank sample | 0 | 0 | 0 |
| The Pericarpium Trichosanthis extract I | 2 | 3 | 3 |
| The Pericarpium Trichosanthis extract II | 2 | 2 | 2 |
| The Pericarpium Trichosanthis extract III | 1 | 1 | 1 |
From above table as can be known, with respect to the cigarette of blank sample, the cigarette excitant of the Fructus Rosae Laevigatae extract that increases on tobacco leaf alleviates, and exhaust gas volumn increases, the raising of sweet property, and the quality of excitant, exhaust gas volumn, sweet property three aspects all is improved.
Above content be in conjunction with concrete preferred implementation to further describing that the present invention did, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, make some substituting or obvious modification without departing from the inventive concept of the premise, and performance or purposes are identical, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. the preparation method of a Fructus Rosae Laevigatae extract is characterized in that: may further comprise the steps:
1) pulverizes: fruit of Cherokee rose raw material to be extracted is dried under 40～70 ℃ of conditions, and be crushed to the fruit of Cherokee rose powder that granularity is 150～420um;
2) alcohol extracting: it is that the mass fraction of 8～15 times of described fruit of Cherokee rose powder qualities is in 50%～80% the ethanolic solution that described fruit of Cherokee rose powder is added quality, refluxing extraction obtains extract, use the described extract of 150～200 purpose screen filtrations to obtain filtrate, described filtrate decompression is concentrated, get the alcohol extract and the dregs of a decoction;
3) water extracts: with adding quality in the described dregs of a decoction after the alcohol extracting is the water of 8～15 times of described dregs of a decoction quality, the backflow lixiviate obtains extract, use the described extract of 150～200 purpose screen filtrations to obtain filtrate, described filtrate decompression concentrate concentrate, add ethanol and make and contain the alcohol amount in the described concentrate and reach 50～75%, stir static 8～24h below 4 ℃, described concentrate filtered obtains filtrate, with described filtrate decompression concentrate aqueous extract;
4) aqueous extract that obtains in alcohol extract that obtains polyamide gels column chromatography separation: with step 2) and the step 3) mixes, obtain total extract, the mass fraction that adds quality and be 4～6 times of described total extract quality in described total extract is that 70～95% ethanolic solution dissolves, obtain lysate, with polyamide gels post on the described lysate, water and/or alcohol flushing are collected eluent;
5) concentrate: it is concentrated that the eluent that obtains in the step 4) is reduced pressure, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.
2. the preparation method of Fructus Rosae Laevigatae extract according to claim 1, it is characterized in that: the consumption of the polyamide gels in the post of polyamide gels described in the step 4) is 1～5 times of described total extract quality, granularity is 60～80 orders, blade diameter length ratio 1:4～the 1:10 of described polyamide gels column chromatography, the absorption flow velocity is 0.5～4.0BV/h.
3. the preparation method of Fructus Rosae Laevigatae extract according to claim 1 is characterized in that: also comprise the film separating step in the step 4): is that the daltonian hollow-fibre membrane of 3k～10k separates with described eluent by molecular weight, collects to see through liquid; Described step 5) reduces pressure concentratedly for the described liquid that sees through that will obtain, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.
4. the preparation method of Fructus Rosae Laevigatae extract according to claim 1, it is characterized in that: also comprise centrifugation step in the step 4): described lysate centrifugation is gone out supernatant, with polyamide gels post on the described supernatant, collect eluent behind water and/or the alcohol flushing.
5. the preparation method of Fructus Rosae Laevigatae extract according to claim 4 is characterized in that: centrifugation rate is 2000～8000rpm during described centrifugation, and the time is 10～30min.
6. the preparation method of Fructus Rosae Laevigatae extract according to claim 1 is characterized in that: step 2) in refluxing extraction obtain extract and be specially: refluxing extraction 1～2 time, each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract; The backflow lixiviate obtains extract and is specially in the step 3): backflow lixiviate 1～2 time, and each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract.
7. the preparation method of Fructus Rosae Laevigatae extract according to claim 1 is characterized in that: step 2) in decompression when concentrating, relative density was 1.12～1.16 to obtain the described alcohol extract and the dregs of a decoction when described filtrate decompression was concentrated into 25 ℃; When decompression concentrates in the step 3), when described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 concentrate; When described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 aqueous extract; When decompression concentrated in the step 5), relative density was 1.20～1.40 when described eluent was evaporated to 25 ℃, and the thick solution after described the concentrating is Fructus Rosae Laevigatae extract.
8. the application of Fructus Rosae Laevigatae extract in cigarette that method according to claim 1 obtains is characterized in that: described Fructus Rosae Laevigatae extract is diluted with the nontoxic organic solvent that has no irritating odor, and extension rate is 10-15 times; Before the preparation cigarette, described Fructus Rosae Laevigatae extract dilution is sprayed at the cured tobacco leaf surface according to the mass ratio of 0.1%-1%.
9. the application of Fructus Rosae Laevigatae extract according to claim 7 in cigarette is characterized in that: described organic solvent is propane diols, sorbierite, xylitol or glycerine, perhaps wherein any mixed liquor more than 2 kinds.
10. a cigarette is characterized in that: be added with the Fructus Rosae Laevigatae extract that method according to claim 1 obtains in the described cigarette.
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| CN103484245A (en) * | 2013-09-18 | 2014-01-01 | 浙江中烟工业有限责任公司 | Directed composite extraction method for tobacco endogenous original fragrant substance |
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| CN111363625A (en) * | 2018-12-25 | 2020-07-03 | 广州华芳烟用香精有限公司 | Preparation method of composite tobacco extract with fragrance styles of pericarpium citri reticulatae and pummelo peel and application of composite tobacco extract in cigarettes |
| CN114788578A (en) * | 2022-05-19 | 2022-07-26 | 湖北中烟工业有限责任公司 | Walnut shell extract for cigarettes, preparation method and cigars |
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| CN103343053A (en) * | 2013-07-23 | 2013-10-09 | 华宝食用香精香料（上海）有限公司 | Tobacco essence capable of improving cigarette taste and fragrance |
| CN103343053B (en) * | 2013-07-23 | 2015-01-28 | 华宝食用香精香料（上海）有限公司 | Tobacco essence capable of improving cigarette taste and fragrance |
| CN103484245A (en) * | 2013-09-18 | 2014-01-01 | 浙江中烟工业有限责任公司 | Directed composite extraction method for tobacco endogenous original fragrant substance |
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| CN110122917A (en) * | 2018-02-08 | 2019-08-16 | 深圳波顿香料有限公司 | A kind of preparation method of preserved citron extract and its application and cigarette in cigarette |
| CN111363625A (en) * | 2018-12-25 | 2020-07-03 | 广州华芳烟用香精有限公司 | Preparation method of composite tobacco extract with fragrance styles of pericarpium citri reticulatae and pummelo peel and application of composite tobacco extract in cigarettes |
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