CN102240067B - Method for preparing fructus fosae laevigatae extracts, application thereof in cigarette and cigarette - Google Patents
Method for preparing fructus fosae laevigatae extracts, application thereof in cigarette and cigarette Download PDFInfo
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Abstract
The invention discloses a method for preparing fructus fosae laevigatae extracts. The fructus fosae laevigatae is extracted by adopting a method combining alcohol and water, so that the effective components in the fructus fosae laevigatae can be extracted more completely; and meanwhile, gel column chromatographic separation is selected in separation, and the specific adsorption action of the polyamide is utilized, so that macromolecule substances, such as tannin and the like which can affect the effects of tobacco adding fragrances can be removed, and the content of the effective components in the fructus fosae laevigatae extract can be ensured. By utilizing the extraction method of the fructus fosae laevigatae, on the one hand, the purification effect can be improved in the production process, and the product quality of the extract product can be improved, thus the whole production process is efficient, and is suitable for industrial implementation; and on the other hand, the fructus fosae laevigatae extract obtained by the preparation method provided by the invention is applied to the preparation of the cigarette, is convenient in use, and can be used for greatly improving the intrinsic quality of the cigarette, thus the stimulation of the cigarette is reduced, the smoke gas is increased, the sweet smoothness of the smoke gas is improved, and the intrinsic quality of the cigarette is greatly improved.
Description
[technical field]
The present invention relates to plant extract and application thereof, the cigarette that particularly relates to a kind of preparation method of Fructus Rosae Laevigatae extract and the application in cigarette thereof and comprise this Fructus Rosae Laevigatae extract.
[background technology]
The fruit of Cherokee rose (Rosa laevigata Mickx) has another name called sugared orange, honeybee sugar bowl, thorn pear, Randia spinosa etc., belongs to rosaceous plant.The fruit of Cherokee rose is conventional Chinese medicine, and medicinal long, determined curative effect is reliable.The Compendium of Material Medica record: fruit of Cherokee rose property is sour, puckery, flat, nontoxic; Return kidney, bladder, large intestine channel.
For the extraction of the fruit of Cherokee rose, in traditional handicraft, have to relate to and adopt ethanol that the fruit of Cherokee rose is extracted, utilize resin to carry out the method that concentrate drying after separation and purification obtains extract.But the Fructus Rosae Laevigatae extract active constituent content that the method obtains is low, is unsuitable for industrializing implementation.And the Fructus Rosae Laevigatae extract that obtains in traditional handicraft is dried powder, also is unsuitable for the long period preservation.How extraction process is improved, the content of the Fructus Rosae Laevigatae extract active ingredient that obtains with raising is the direction that people make great efforts.On the other hand, present stage, because the pharmacological action of the fruit of Cherokee rose, the application study of the domestic relevant fruit of Cherokee rose mainly concentrates on the medicines and health protection field, and is less in the application of other field, and the application in cigarette has no relevant report.
[summary of the invention]
Technical problem to be solved by this invention is: make up above-mentioned the deficiencies in the prior art, propose a kind of preparation method of Fructus Rosae Laevigatae extract, can improve by a relatively large margin the content of active ingredient in the Fructus Rosae Laevigatae extract that obtains.
Another technical problem to be solved by this invention is: Fructus Rosae Laevigatae extract is applied to can greatly improve the interior quality of cigarette in the cigarette manufacturing.
Technical problem of the present invention is solved by following technical scheme:
A kind of preparation method of Fructus Rosae Laevigatae extract comprises the following steps: 1) pulverize: fruit of Cherokee rose raw material to be extracted is dried under 40～70 ℃ of conditions, and be crushed to the fruit of Cherokee rose powder that granularity is 150～420um; 2) alcohol extracting: it is that the mass fraction of 8～15 times of described fruit of Cherokee rose powder qualities is in 50%～80% ethanolic solution that described fruit of Cherokee rose powder is added quality, refluxing extraction obtains extract, use 150～200 described extracts of purpose screen filtration to obtain filtrate, described filtrate decompression is concentrated, get alcohol extract and the dregs of a decoction; 3) water extraction is got: be the water of 8～15 times of described dregs of a decoction quality with adding quality in the described dregs of a decoction after alcohol extracting, the backflow lixiviate obtains extract, use 150～200 described extracts of purpose screen filtration to obtain filtrate, described filtrate decompression concentrates to get concentrate, add ethanol to make to contain in described concentrate the alcohol amount to reach 50～75%, stir, static 8～24h below 4 ℃, described concentrate is filtered obtain filtrate, described filtrate decompression is concentrated to get aqueous extract; 4) aqueous extract that obtains in the alcohol extract that obtains polyamide gels column chromatography separation: with step 2) and step 3) mixes, obtain total extract, adding quality in described total extract is that the mass fraction of 4～6 times of described total extract quality is that 70～95% ethanolic solution dissolves, obtain lysate, with polyamide gels post on described lysate, water and/or alcohol flushing are collected eluent; 5) concentrated: the eluent that obtains in step 4) is carried out reduced pressure concentration, and the thick solution after described concentrating is Fructus Rosae Laevigatae extract.
In preferred technical scheme,
The consumption of the polyamide gels in the post of polyamide gels described in step 4) is 1～5 times of described total extract quality, and granularity is 60～80 orders, the blade diameter length ratio 1:4～1:10 of described polyamide gels column chromatography, and the absorption flow velocity is 0.5～4.0BV/h.
Also comprise the film separating step in step 4): be the daltonian Middle hollow fiber membrane of 3k～10k with described eluent by molecular weight, collect and see through liquid; Described step 5) is carried out reduced pressure concentration for the described liquid that sees through that will obtain, and the thick solution after described concentrating is Fructus Rosae Laevigatae extract.Like this, by increasing the film separating step, can further selectively remove some macromolecular substances, improve the content that extracts the fruit of Cherokee rose active ingredient that obtains.
Also comprise centrifugation step in step 4): described lysate centrifugation is gone out supernatant, with polyamide gels post on described supernatant, collect eluent after water and/or alcohol flushing.By before the polyamide gels column chromatography, centrifugation step being set, make total extract have higher clarity and stability, can avoid stopping up filler when upper polyamide gels column chromatography, reduce the situation of chromatography efficient.
During described centrifugation, centrifugation rate is 2000～8000rpm, and the time is 10～30min.
Step 2) in, refluxing extraction obtains extract and is specially: refluxing extraction 1～2 time, and each 0.5h～3h, the solution that each time extraction is obtained merge and obtain extract; In step 3), the backflow lixiviate obtains extract and is specially: backflow lixiviate 1～2 time, each 0.5h～3h, the solution that each time extraction is obtained merge and obtain extract.
Step 2) in during reduced pressure concentration, when described filtrate decompression is concentrated into 25 ℃, relative density is 1.12～1.16 to obtain described alcohol extract and the dregs of a decoction; In step 3) during reduced pressure concentration, when described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 concentrate; When described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 aqueous extract; In step 5), during reduced pressure concentration, when described eluent is evaporated to 25 ℃, relative density is 1.20～1.40, and described thick solution after concentrated is Fructus Rosae Laevigatae extract.Control through this reduced pressure concentration, the extract product that obtains can reach certain viscosity, and still has certain mobility, does not generally need to add anticorrisive agent under this state in extract product and just can preserve the long period.Namely make the Fructus Rosae Laevigatae extract after concentrating that better stability is arranged.
Technical problem of the present invention is solved by following further technical scheme:
A kind of application of Fructus Rosae Laevigatae extract in cigarette that obtains according to above-mentioned method diluted described Fructus Rosae Laevigatae extract with the nontoxic organic solvent that has no irritating odor, extension rate is 10-15 times; Before the preparation cigarette, described Fructus Rosae Laevigatae extract dilution is sprayed at the cured tobacco leaf surface according to the mass ratio of 0.1%-1%.
Described organic solvent is propane diols, sorbierite, xylitol or glycerine, perhaps any mixed liquor more than 2 kinds wherein.
Technical problem of the present invention is solved by following further technical scheme:
A kind of cigarette is added with the Fructus Rosae Laevigatae extract that obtains according to above-mentioned method in described cigarette.
The beneficial effect that the present invention is compared with the prior art is:
The extracting method of the fruit of Cherokee rose of the present invention, adopt alcohol, water to extract the fruit of Cherokee rose in conjunction with the method for extracting, make the active ingredient in the fruit of Cherokee rose can extract comparatively fully, select gel column chromatography to separate when separating simultaneously, utilize the specific adsorption effect of polyamide, can remove most of macromolecular substances such as tannin that affect the tobacco flavoring effect in rear extended meeting, guarantee the content of active ingredient in Fructus Rosae Laevigatae extract.The extracting method of the fruit of Cherokee rose of the present invention can be improved the purification effect in production process, improve to extract the product quality of product, makes in whole production process efficiently, is suitable for industrializing implementation.On the other hand, the resulting Fructus Rosae Laevigatae extract of preparation method of the present invention is applied in the cigarette preparation, easy to use, and can greatly improve the interior quality of cigarette, the cigarette excitant is descended, exhaust gas volumn increases, the sweet property raising of flue gas has greatly improved the interior quality of cigarette.
[specific embodiment]
Below in conjunction with the specific embodiment, the present invention is described in further details.
A kind of preparation method of Fructus Rosae Laevigatae extract comprises the following steps:
1) pulverize: fruit of Cherokee rose raw material to be extracted is dried under 40～70 ℃ of conditions, and be crushed to the meal that granularity is 150～420um.Raw material is pulverized as meal, made the fruit of Cherokee rose that higher recovery rate be arranged in subsequent extracted.
2) alcohol extracting: it is that the mass fraction of 8～15 times of fruit of Cherokee rose powder qualities is in 50%～80% ethanolic solution that fruit of Cherokee rose powder is added quality, refluxing extraction obtains extract, use 150～200 purpose screen filtration extracts to obtain filtrate, filtrate decompression is concentrated, get alcohol extract and the dregs of a decoction.Wherein, specifically can be during refluxing extraction: refluxing extraction 1～2 time, each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract.
3) water extraction is got: with step 2) to add quality in the dregs of a decoction that obtain after alcohol extracting be the water of 8～15 times of described dregs of a decoction quality, the backflow lixiviate obtains extract, use 150～200 purpose screen filtration extracts to obtain filtrate, the concentrated concentrate that to get of filtrate decompression adds ethanol to make to contain the alcohol amount in concentrate to reach 50～75%, stirs, static 8～24h below 4 ℃, concentrate is filtered obtain filtrate, filtrate decompression is concentrated, get aqueous extract.Wherein, the backflow lixiviate obtains extract and is specially: backflow lixiviate 1～2 time, and each 0.5h～3h, the solution merging that each time extraction is obtained obtains extract.
Above-mentioned employing alcohol, water extract the fruit of Cherokee rose in conjunction with the method for extracting, and the composition in the fruit of Cherokee rose can be extracted comparatively fully, make extract all can obtain good effect at color and luster and follow-up filtration difficulty or ease, the final aspects such as active constituent content that obtain of extracting.
4) aqueous extract that obtains in the alcohol extract that obtains polyamide gels column chromatography separation: with step 2) and step 3) mixes, obtain total extract, adding quality in total extract is that the mass fraction of 4～6 times of total extract quality is that 70～95% ethanolic solution dissolves, get lysate, with polyamide gels post on described lysate, water and/or alcohol flushing are collected eluent.Wherein, the consumption of the polyamide gels in the polyamide gels post is 1～5 times of total extract quality, and granularity is 60～80 orders, the blade diameter length ratio 1:4～1:10 of polyamide gels column chromatography, and the absorption flow velocity is 0.5～4.0BV/h.Through the polyamide gels column chromatography, utilize the specific adsorption effect of polyamide, can remove most of macromolecular substances such as tannin that affect the tobacco flavoring effect in rear extended meeting.
Preferably, also comprise centrifugation step in step 4): described lysate centrifugation is gone out supernatant, with polyamide gels post on described supernatant, collect eluent after water and/or alcohol flushing.Centrifugal before the polyamide gels column chromatography makes total extract have higher clarity and stability, can avoid stopping up filler when upper polyamide gels column chromatography, reduces the situation of chromatography efficient.Wherein, during centrifugation, centrifugation rate is 2000～8000rpm, and the time is 10～30min.
5) concentrated: the eluent that obtains in step 4) is carried out reduced pressure concentration, and the thick solution after concentrating is Fructus Rosae Laevigatae extract.
Preferably, also comprise the film separating step in step 4): be the daltonian Middle hollow fiber membrane of 3k～10k with eluent by molecular weight, collect and see through liquid.Described step 5) is the described liquid that sees through that will obtain and carries out reduced pressure concentration, and the thick solution after described concentrating is Fructus Rosae Laevigatae extract.Increase the film separating step in step 4), can further selectively remove some macromolecular substances, improve the content that extracts the fruit of Cherokee rose active ingredient that obtains.
Further preferably, in above steps, during reduced pressure concentration, 2) in during reduced pressure concentration, when described filtrate decompression is concentrated into 25 ℃, relative density is 1.12～1.16 to obtain described alcohol extract and the dregs of a decoction; In step 3) during reduced pressure concentration, when described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 concentrate; When described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 aqueous extract; In step 5), during reduced pressure concentration, when described eluent is evaporated to 25 ℃, relative density is 1.20～1.40, and described thick solution after concentrated is Fructus Rosae Laevigatae extract.Control through this reduced pressure concentration, the extract product that obtains can reach certain viscosity, and still has certain mobility, does not generally need to add anticorrisive agent under this state in extract product and just can preserve the long period.Namely make the Fructus Rosae Laevigatae extract after concentrating that better stability is arranged.
The Fructus Rosae Laevigatae extract that obtains in above-mentioned preparation method, adopt alcohol, water to extract the fruit of Cherokee rose in conjunction with the method for extracting, make the active ingredient in the fruit of Cherokee rose can extract comparatively fully, select gel column chromatography to separate when separating simultaneously, utilize the specific adsorption effect of polyamide, can remove most of macromolecular substances such as tannin that affect the tobacco flavoring effect in rear extended meeting, guarantee the content of active ingredient in Fructus Rosae Laevigatae extract.
The Fructus Rosae Laevigatae extract that obtains according to the method described above is applied in cigarette, at first, Fructus Rosae Laevigatae extract is diluted with the nontoxic organic solvent that has no irritating odor, extension rate is 10-15 times.Wherein, organic solvent can be propane diols, sorbierite, xylitol or glycerine, perhaps any mixed liquor more than 2 kinds wherein.Secondly, before the preparation cigarette, the Fructus Rosae Laevigatae extract dilution is sprayed at the cured tobacco leaf surface according to the mass ratio of 0.1%-1%.Then, cigarette process is routinely processed, and namely after spraying the dilution of Fructus Rosae Laevigatae extract, with flue-cured tobacco tobacco storage 4 hours, then makes cigarette finished product through chopping, baking silk, perfuming, storage silk, cigarette and packaging process.
Embodiment 1
Accurately take the 200g fruit of Cherokee rose, 45 ℃ of oven dry, and be crushed to the meal that granularity is 250um, adding 1.6kg concentration is 60% ethanolic solution, refluxing extraction 2 times, each 1h, extract filters through 200 eye mesh screens, and when being evaporated to 25 ℃, relative density is 1.12, gets alcohol extract; The water refluxing extraction 1 time that adds 2kg in the dregs of a decoction, extract 2h, extract filters through 200 eye mesh screens, when being evaporated to 25 ℃, relative density is 1.12, adds ethanol to make to contain the alcohol amount to reach 65%, stirs, static 10h below 4 ℃, filter, decompression filtrate recycling ethanol and when being concentrated into 25 ℃ relative density be 1.14, get aqueous extract; Merging alcohol extract and aqueous extract, add the 80% ethanol dissolving of 4 times, is centrifugation 20min under 4000rpm at centrifugation rate, obtains supernatant; Supernatant is by polyamide gels post Dynamic Adsorption, and water and/or alcohol flushing are collected eluent, and wherein the consumption of polyamide gels is 1.5 times of total extract quality, and granularity is 60～80 orders, and the blade diameter length ratio of chromatography is 1:8, and the absorption flow velocity is 1BV/h; Eluent is the daltonian hollow-fibre membrane of 5k through molecular cut off again, collects and sees through liquid, and when being evaporated to 25 ℃, relative density is 1.25, is the Fructus Rosae Laevigatae extract I.
Embodiment 2
Accurately take the 200g fruit of Cherokee rose, 50 ℃ of oven dry, and be crushed to the meal that granularity is 420um, adding 2.4kg concentration is 70% ethanolic solution, and refluxing extraction 1 time is extracted 3h, extract filters through 200 eye mesh screens, and when being evaporated to 25 ℃, relative density is 1.15, gets alcohol extract; The water refluxing extraction 1 time that adds 2.4kg in the dregs of a decoction, extract 3h, extract filters through 200 eye mesh screens, when being evaporated to 25 ℃, relative density is 1.14, adds ethanol to make to contain the alcohol amount to reach 75%, stirs, static 8h below 4 ℃, filter, decompression filtrate recycling ethanol and when being concentrated into 25 ℃ relative density be 1.13, get aqueous extract; Merging alcohol extract and aqueous extract, add the 95% ethanol dissolving of 3 times, is centrifugation 10min under 6000rpm at centrifugation rate, obtains supernatant; Supernatant is by the polyamide gels Dynamic Adsorption, and water and/or alcohol flushing are collected eluent, and wherein the consumption of polyamide gels is 2 times of total extract, and granularity is 60～80 orders, and the blade diameter length ratio of chromatography is 1:4, and the absorption flow velocity is 0.5BV/h; Eluent is the daltonian hollow-fibre membrane of 3k through molecular cut off again, collects and sees through liquid, and when being evaporated to 25 ℃, relative density is 1.31, is the Fructus Rosae Laevigatae extract II.
Embodiment 3
Accurately take the 200g fruit of Cherokee rose, 65 ℃ of oven dry, and be crushed to the meal that granularity is 250um, adding 1.6kg concentration is 50% ethanolic solution, refluxing extraction 2 times, each 2h, extract filters through 200 eye mesh screens, and when being evaporated to 25 ℃, relative density is 1.12, gets alcohol extract; The water refluxing extraction 2 times that adds 1.6kg in the dregs of a decoction, each 1h that extracts, extract filters through 200 eye mesh screens, when being evaporated to 25 ℃, relative density is 1.14, adds ethanol to make to contain the alcohol amount to reach 50%, stirs, static 16h below 4 ℃, filter, decompression filtrate recycling ethanol and when being concentrated into 25 ℃ relative density be 1.13, get aqueous extract; Merging alcohol extract and aqueous extract, add the 75% ethanol dissolving of 3 times, is centrifugation 15min under 5000rpm at centrifugation rate, obtains supernatant; Supernatant is by the polyamide gels Dynamic Adsorption, and water and/or alcohol flushing are collected eluent, and wherein the consumption of polyamide gels is 3 times of total extract, and granularity is 60～80 orders, and the blade diameter length ratio of chromatography is 1:10, and the absorption flow velocity is 2.5BV/h; Eluent is the daltonian hollow-fibre membrane of 8k through molecular cut off again, collects and sees through liquid, and when being evaporated to 25 ℃, relative density is 1.33, is the Fructus Rosae Laevigatae extract III.
Experimental test: the Fructus Rosae Laevigatae extract I, II, the III that make in above-described embodiment 1,2 and 3 all are diluted to 10 times with 50% propylene glycol solution, then are sprayed at the middle-grade blank of same (not flavoring and casing) flue-cured tobacco pipe tobacco surperficial, addition is 0.5% of pipe tobacco.Subsequent treatment cigarette process routinely carries out, and namely after spraying the dilution of Fructus Rosae Laevigatae extract, with flue-cured tobacco tobacco storage 4 hours, then makes cigarette finished product through chopping, baking silk, perfuming, storage silk, cigarette and packaging process.To the contrast of smokeing panel test of above-mentioned sample, the overall sensory test result that obtains is as shown in the table respectively in the group of smokeing panel test that is comprised of 8 people.Wherein, the interior quality of blank sample all is set as 0, improves to some extent (alleviate as excitant, exhaust gas volumn increases, and sweet sense improves) with respect to the blank sample interior quality, mutually deservedly just divide, score value is higher, and it is better to improve; Decrease (increase as excitant, exhaust gas volumn reduces, and sweet sense reduces) with respect to the blank sample interior quality, mutually deserved negative minute, negative minute lower, and it is poorer to improve.
From above table as can be known, with respect to the cigarette of blank sample, the cigarette excitant of the Fructus Rosae Laevigatae extract that increases on tobacco leaf alleviates, and exhaust gas volumn increases, the raising of sweet property, and the quality of excitant, exhaust gas volumn, sweet property three aspects: all is improved.
Above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, make without departing from the inventive concept of the premise some substituting or obvious modification, and performance or purposes identical, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. the preparation method of a Fructus Rosae Laevigatae extract is characterized in that: comprise the following steps:
1) pulverize: fruit of Cherokee rose raw material to be extracted is dried under 40～70 ℃ of conditions, and be crushed to the fruit of Cherokee rose powder that granularity is 150～420um;
2) alcohol extracting: it is that the mass fraction of 8～15 times of described fruit of Cherokee rose powder qualities is in 50%～80% ethanolic solution that described fruit of Cherokee rose powder is added quality, refluxing extraction obtains extract, use 150～200 described extracts of purpose screen filtration to obtain filtrate, described filtrate decompression is concentrated, get alcohol extract and the dregs of a decoction;
3) water extraction is got: be the water of 8～15 times of described dregs of a decoction quality with adding quality in the described dregs of a decoction after alcohol extracting, the backflow lixiviate obtains extract, use 150～200 described extracts of purpose screen filtration to obtain filtrate, described filtrate decompression concentrates to get concentrate, add ethanol to make to contain in described concentrate the alcohol amount to reach 50～75%, stir, static 8～24h below 4 ℃, described concentrate is filtered obtain filtrate, described filtrate decompression is concentrated to get aqueous extract;
4) aqueous extract that obtains in the alcohol extract that obtains polyamide gels column chromatography separation: with step 2) and step 3) mixes, obtain total extract, adding quality in described total extract is that the mass fraction of 4～6 times of described total extract quality is that 70～95% ethanolic solution dissolves, obtain lysate, with polyamide gels post on described lysate, water and/or alcohol flushing are collected eluent;
5) concentrated: the eluent that obtains in step 4) is carried out reduced pressure concentration, and the thick solution after described concentrating is Fructus Rosae Laevigatae extract.
2. the preparation method of Fructus Rosae Laevigatae extract according to claim 1, it is characterized in that: the consumption of the polyamide gels in the post of polyamide gels described in step 4) is 1～5 times of described total extract quality, granularity is 60～80 orders, blade diameter length ratio 1:4～the 1:10 of described polyamide gels column chromatography, the absorption flow velocity is 0.5～4.0BV/h.
3. the preparation method of Fructus Rosae Laevigatae extract according to claim 1, is characterized in that: also comprise the film separating step in step 4): be the daltonian Middle hollow fiber membrane of 3k～10k with described eluent by molecular weight, collect and see through liquid; Described step 5) is carried out reduced pressure concentration for the described liquid that sees through that will obtain, and the thick solution after described concentrating is Fructus Rosae Laevigatae extract.
4. the preparation method of Fructus Rosae Laevigatae extract according to claim 1, it is characterized in that: also comprise centrifugation step in step 4): described lysate centrifugation is gone out supernatant, with polyamide gels post on described supernatant, collect eluent after water and/or alcohol flushing.
5. the preparation method of Fructus Rosae Laevigatae extract according to claim 4, it is characterized in that: during described centrifugation, centrifugation rate is 2000～8000rpm, the time is 10～30min.
6. the preparation method of Fructus Rosae Laevigatae extract according to claim 1, is characterized in that: step 2) in refluxing extraction obtain extract and be specially: refluxing extraction 1～2 time, each 0.5h～3h, the solution that each time extraction is obtained merge and obtain extract; In step 3), the backflow lixiviate obtains extract and is specially: backflow lixiviate 1～2 time, each 0.5h～3h, the solution that each time extraction is obtained merge and obtain extract.
7. the preparation method of Fructus Rosae Laevigatae extract according to claim 1, is characterized in that: step 2) in during reduced pressure concentration, when described filtrate decompression is concentrated into 25 ℃, relative density is 1.12～1.16 to obtain described alcohol extract and the dregs of a decoction; In step 3) during reduced pressure concentration, when described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 concentrate; When described filtrate decompression is concentrated into 25 ℃ relative density be 1.12～1.16 aqueous extract; In step 5), during reduced pressure concentration, when described eluent is evaporated to 25 ℃, relative density is 1.20～1.40, and described thick solution after concentrated is Fructus Rosae Laevigatae extract.
8. the application of Fructus Rosae Laevigatae extract in cigarette that method according to claim 1 obtains is characterized in that: described Fructus Rosae Laevigatae extract is diluted with the nontoxic organic solvent that has no irritating odor, and extension rate is 10-15 times; Before the preparation cigarette, described Fructus Rosae Laevigatae extract dilution is sprayed at the cured tobacco leaf surface according to the mass ratio of 0.1%-1%.
9. the application of Fructus Rosae Laevigatae extract according to claim 8 in cigarette is characterized in that: described organic solvent is propane diols, sorbierite, xylitol or glycerine, perhaps any mixed liquor more than 2 kinds wherein.
10. a cigarette, is characterized in that: be added with the Fructus Rosae Laevigatae extract that method according to claim 1 obtains in described cigarette.
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| CN101036711A (en) * | 2007-03-12 | 2007-09-19 | 安徽师范大学 | Technique of extracting polysaccharide in rosa laevigata michx |
| CN101385793A (en) * | 2008-10-22 | 2009-03-18 | 大连医科大学 | High-purity extraction method of total flavone from cherokee rose and use thereof in preparing medicine for treating cardio-cerebrovascular disease |
| CN101829214A (en) * | 2010-05-21 | 2010-09-15 | 中国人民解放军第二军医大学 | Cherokee rose leaf extract and application thereof in preparing medicine capable of curing burn and scald |
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