CN102217583A - Method for collecting small-sized foraging insects of rice - Google Patents
Method for collecting small-sized foraging insects of rice Download PDFInfo
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- CN102217583A CN102217583A CN 201110096009 CN201110096009A CN102217583A CN 102217583 A CN102217583 A CN 102217583A CN 201110096009 CN201110096009 CN 201110096009 CN 201110096009 A CN201110096009 A CN 201110096009A CN 102217583 A CN102217583 A CN 102217583A
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- plastic bag
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- insect
- insects
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Abstract
The invention relates to the field of bioresearch foundation, and provides a method for collecting small-sized foraging insects of rice. The method comprises the following steps of: taking a colorless transparent polyethylene plastic bag, and inversely covering the plastic bag on ears of flowering rice, wherein the distance between the bottom of the plastic bag and the tops of the ears of rice is at least 2cm; slightly tapping rice stems, and slightly removing the plastic bag after insects fly onto the wall of the plastic bag; and singly transferring the insects of which body lengths are less than 0.2cm to centrifuge tubes, and adding stationary liquid into the tubes, wherein the stationary liquid is prepared by mixing glacial acetic acid and ethanol in a volume ratio of 1:3. The polyethylene plastic bag is light and convenient to carry; the plastic bag is provided with a sealing bar, so the plastic bag is convenient to use and is suitable for field work; and during insect catching, the catching of single small-sized insects (body lengths are less than 0.2cm) can be easily realized, so that the work efficiency is greatly improved.
Description
Technical field
The invention belongs to the biological study basic field, be specifically related to a kind of method of gathering the small-sized visit flower of paddy rice insect.
Background technology
Present insect collection method mainly by net sweep, Ma Shi net is gathered, lure collection, trap etc.The importance of research insect pollinator is the pollen quantity that will know that insect is carried on one's body, this is an important indicator weighing the insect pollination ability, but the insect of present method collection mixes more, the situation that pollen infects can appear each other, the pollen amount that carries of single head worm can't be accurately added up, thereby this research needs can't be satisfied.
Summary of the invention
The technical problem to be solved in the present invention is, overcomes deficiency of the prior art, and a kind of method of gathering the small-sized visit flower of paddy rice insect is provided.
Be the technical solution problem, solution of the present invention is:
Propose a kind of method of gathering the small-sized visit flower of paddy rice insect, comprising:
Get the water white transparency polyethylene plastic bag, plastic sack covered on the spike of rice of the paddy rice that blooms, between plastic sack bottom and the spike of rice top apart from 2cm at least; Bounce the rice bar gently, treat that insect flies to the plastic sack wall after, remove plastic sack gently; The insect single head of body length＜0.2cm is transferred to centrifuge tube, adds fixer in the pipe; Described fixer was mixed by glacial acetic acid and ethanol in 1: 3 by volume.
Among the present invention, described polyethylene plastic bag: long 25.0cm, wide 17.0cm, transparency 〉=85%.、
Among the present invention, described polyethylene plastic bag is established sealing strip.
Beneficial effect of the present invention is: because of the polyethylene plastic bag material is light, be easy to carry; It is easy to use that plastic sack has sealing strip, is fit to field work; When insect-catching, can realize easily the single head small insects (body is long＜0.2cm) seizure, greatly improved operating efficiency.
Description of drawings
Fig. 1 is for gathering the plastic sack of usefulness;
Fig. 2 is the collecting method schematic diagram.
Embodiment
Select a kind of colourless transparent plastic bag of making by polyethylene (long: 25.0cm, wide: 17.0cm, transparency 〉=85%), during collection, plastic sack is covered in as shown in Figure 2 on the spike of rice of the paddy rice that blooms, bounce the rice bar gently, after treating that insect flies to the plastic sack wall, remove plastic sack gently, with dotted line as shown in Figure 2 with the insect on top (body is long＜0.2cm) single head is transferred to centrifuge tube, adds fixer in the pipe.Worm and pollen are taken back indoor, wash pollen with fixer, are filtered on the smooth blank sheet of paper, at microscopically statistics pollen amount.
Claims (3)
1. method of gathering the small-sized visit of paddy rice flower insect comprises:
Get the water white transparency polyethylene plastic bag, plastic sack covered on the spike of rice of the paddy rice that blooms, between plastic sack bottom and the spike of rice top apart from 2cm at least; Bounce the rice bar gently, treat that insect flies to the plastic sack wall after, remove plastic sack gently; The insect single head of body length＜0.2cm is transferred to centrifuge tube, adds fixer in the pipe; Described fixer was mixed by glacial acetic acid and ethanol in 1: 3 by volume.
2. the method for the small-sized visit flower of collection paddy rice according to claim 1 insect is characterized in that described polyethylene plastic bag: long 25.0cm, wide 17.0cm, transparency 〉=85%.
3. the method for the small-sized visit flower of collection paddy rice according to claim 1 insect is characterized in that described polyethylene plastic bag is established sealing strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110096009 CN102217583A (en) | 2011-04-21 | 2011-04-21 | Method for collecting small-sized foraging insects of rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110096009 CN102217583A (en) | 2011-04-21 | 2011-04-21 | Method for collecting small-sized foraging insects of rice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102217583A true CN102217583A (en) | 2011-10-19 |
Family
ID=44774497
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110096009 Pending CN102217583A (en) | 2011-04-21 | 2011-04-21 | Method for collecting small-sized foraging insects of rice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102217583A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860292A (en) * | 2012-10-17 | 2013-01-09 | 云南省农业科学院蚕桑蜜蜂研究所 | Method for collecting and receiving Pealius mori adults in biological assay |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2091540U (en) * | 1991-01-22 | 1992-01-01 | 财李孙产业株式会社 | Insects collector and implements |
| CN101053321A (en) * | 2007-05-30 | 2007-10-17 | 盛茂领 | Trunk insect device |
-
2011
- 2011-04-21 CN CN 201110096009 patent/CN102217583A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2091540U (en) * | 1991-01-22 | 1992-01-01 | 财李孙产业株式会社 | Insects collector and implements |
| CN101053321A (en) * | 2007-05-30 | 2007-10-17 | 盛茂领 | Trunk insect device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860292A (en) * | 2012-10-17 | 2013-01-09 | 云南省农业科学院蚕桑蜜蜂研究所 | Method for collecting and receiving Pealius mori adults in biological assay |
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Legal Events
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111019 |