CN102210355A - Method for enzymatic synthesis of corn oil rich in caprylin - Google Patents
Method for enzymatic synthesis of corn oil rich in caprylin Download PDFInfo
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- CN102210355A CN102210355A CN 201110081424 CN201110081424A CN102210355A CN 102210355 A CN102210355 A CN 102210355A CN 201110081424 CN201110081424 CN 201110081424 CN 201110081424 A CN201110081424 A CN 201110081424A CN 102210355 A CN102210355 A CN 102210355A
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- corn oil
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Abstract
The invention relates to a method for enzymatic synthesis of corn oil rich in caprylin. By adopting the method, the problem that the method for enzymatic synthesis of the corn oil rich in the caprylin by utilizing octanoic acid and the corn oil is absent at present can be solved. The method comprises the following steps: placing the octanoic acid, the corn oil and lipase into an autoclave, performing reaction in a water bath pot, taking out a product after the end of the reaction, and centrifugating, thereby getting the corn oil rich in the caprylin. Gas chromatography analysis proves that in the composition of fatty acid of the reaction product, the content of medium chain fatty acid with physiological activity can be as high as 34.02%, and the functional characteristic of the corn oil is enhanced. By adopting the method for enzymatic synthesis of the corn oil rich in the caprylin, the reaction is rapid, the pollution is avoided, and the method can be used in trial production in the food industry.
Description
Technical field:
The present invention relates to the synthetic method that is rich in caprylin corn oil of a kind of enzyme process
Background technology:
Foreign study is found in recent years, edible medium chain fatty acid can reduce body weight and body fat content, the consumption of promotion energy in the meals, some country even clearly with the health food of middle LCFA Related product authentication for having specific function replaces LCFA can effectively reduce healthy human body fat aggregation and blood triglyceride levels after the meal with medium chain fatty acid.
Corn oil claims maize germ oil again, and wherein unsaturated fatty acid content is mainly linoleic plus oleic acid more than 80%, and linoleic acid accounts for more than 50% of grease total amount, is rich in vitamin E, phosphatide etc., is a kind of high-quality edible oil.Linoleic acid is the essential fatty acid that human body self can not synthesize, it has the body's cholesterol of reduction, hypotensive, softening blood vessel, prevention and improve artery sclerosis reduces effects such as heart disease generation, and can alleviate the outbreak of human body prostatic disorder and the generation of dermatitis.Because the function of natural B B-complex E, corn oil all has tangible curative effect and prevention effect to heart disease, thrombophlebitis, muscular atrophy, trophism encephalomalacia.
The aliphatic acid that does not contain medium chain in the corn oil, but also do not utilize sad at present and the synthetic method that is rich in caprylin corn oil of corn oil enzyme process.The present invention adopts the synthetic corn oil that is rich in caprylin of enzyme process, is swift in response, pollution-free, and method can be used for producing as a trial in the food industry.
Summary of the invention:
The invention provides the synthetic method that is rich in caprylin corn oil of enzyme process, also do not utilize sad at present and the synthetic problem that is rich in the method for caprylin corn oil of corn oil enzyme process, strengthened the functional characteristic of corn oil to solve.
Step of the present invention is as follows:
Step 1: the processing before the reaction: the molar ratio by 3: 1 in stainless steel autoclave adds sad and corn oil, adds the lipase Novozym 435 of gross mass 3%～5% simultaneously, adds and stirs magnetic bead;
Step 2: preheating: stainless steel autoclave is put into 45～65 ℃ of thermostat water bath preheating 20min;
Step 3: add thermal response: stainless steel autoclave is put into 60 ℃ of thermostat water baths, start magnetic stirring apparatus under the condition of constant temperature and pressure, speed of agitator is 120r/min, and the reaction time is 20～28h;
Step 4: reaction finishes: reaction is cooled to room temperature with stainless steel autoclave after finishing, and temperature fall time is
Step 4: reaction finishes: reaction is cooled to room temperature with stainless steel autoclave after finishing, and temperature fall time is 1～2h, emits gas, opens stainless steel autoclave, takes out the product that obtains;
Step 5: centrifugal: place centrifuge centrifugal the product of taking out, rotating speed is 4000r/min, and the time is 10～25min;
Step 6: promptly get product after taking out supernatant.
Through gas phase analysis, during the aliphatic acid of product was formed, the medium chain fatty acid content with physiologically active had strengthened the functional characteristic of corn oil up to 34.02%.The present invention adopts the synthetic corn oil that is rich in caprylin of enzyme process, is swift in response, pollution-free, and method can be used for producing as a trial in the food industry.
The specific embodiment:
The specific embodiment one: present embodiment is as follows:
Step 1: the processing before the reaction: the molar ratio by 3: 1 in stainless steel autoclave adds sad and corn oil, adds the lipase Novozym 435 of gross mass 3%～5% simultaneously, adds and stirs magnetic bead;
Step 2: preheating: stainless steel autoclave is put into 45～65 ℃ of thermostat water bath preheating 20min;
Step 3: add thermal response: stainless steel autoclave is put into 60 ℃ of thermostat water baths, start magnetic stirring apparatus under the condition of constant temperature and pressure, speed of agitator is 120r/min, and the reaction time is 20～28h;
Step 4: reaction finishes: reaction is cooled to room temperature with stainless steel autoclave after finishing, and temperature fall time is 1～2h, emits gas, opens stainless steel autoclave, takes out the product that obtains;
Step 5: centrifugal: place centrifuge centrifugal the product of taking out, rotating speed is 4000r/min, and the time is 10～25min;
Step 6: promptly get product after taking out supernatant.
The specific embodiment two: present embodiment and the specific embodiment one difference are that the addition of lipase Novozym 435 in the step 1 is 4% of a sad and corn oil gross mass, and other composition is identical with the specific embodiment one with step.
The specific embodiment three: present embodiment and the specific embodiment one difference are that the temperature of thermostat water bath in the step 2 is 60 ℃, and other composition is identical with the specific embodiment one with step.
The specific embodiment four: present embodiment and the specific embodiment one difference are that the reaction time is 22h in the step 6, and other composition is identical with the specific embodiment one with step.
The specific embodiment five: present embodiment and the specific embodiment one difference are that centrifugation time is 15min in the step 6.Other composition is identical with the specific embodiment one with step.
Claims (5)
1. an enzyme process synthesizes the method that is rich in caprylin corn oil, it is characterized in that its step is as follows:
Step 1: the processing before the reaction: the molar ratio by 3: 1 in stainless steel autoclave adds sad and corn oil, adds the lipase Novozym 435 of gross mass 3%～5% simultaneously, adds and stirs magnetic bead;
Step 2: preheating: stainless steel autoclave is put into 45～65 ℃ of thermostat water bath preheating 20min;
Step 3: add thermal response: stainless steel autoclave is put into 60 ℃ of thermostat water baths, start magnetic stirring apparatus under the condition of constant temperature and pressure, speed of agitator is 120r/min, and the reaction time is 20～28h;
Step 4: reaction finishes: reaction is cooled to room temperature with stainless steel autoclave after finishing, and temperature fall time is 1～2h, emits gas, opens stainless steel autoclave, takes out the product that obtains;
Step 5: centrifugal: place centrifuge centrifugal the product of taking out, rotating speed is 4000r/min, and the time is 10～25min;
Step 6: promptly get product after taking out supernatant.
2. the synthetic method that is rich in caprylin corn oil of a kind of enzyme process according to claim 1, the addition that it is characterized in that lipase Novozym 435 in the step 1 be sad and the corn oil gross mass 4%;
3. the synthetic method that is rich in caprylin corn oil of a kind of enzyme process according to claim 1, the temperature that it is characterized in that thermostat water bath in the step 5 is 60 ℃;
4. the synthetic method that is rich in caprylin corn oil of a kind of enzyme process according to claim 1 is characterized in that the reaction time is 22h in the step 6;
5. the synthetic method that is rich in caprylin corn oil of a kind of enzyme process according to claim 1 is characterized in that centrifugation time is 15min in the step 8.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110081424 CN102210355A (en) | 2011-04-01 | 2011-04-01 | Method for enzymatic synthesis of corn oil rich in caprylin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110081424 CN102210355A (en) | 2011-04-01 | 2011-04-01 | Method for enzymatic synthesis of corn oil rich in caprylin |
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| CN102210355A true CN102210355A (en) | 2011-10-12 |
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| CN 201110081424 Pending CN102210355A (en) | 2011-04-01 | 2011-04-01 | Method for enzymatic synthesis of corn oil rich in caprylin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492743A (en) * | 2011-11-28 | 2012-06-13 | 浙江工业大学 | Method for preparing medium-chain triglyceride from ionic liquid |
| CN104642570A (en) * | 2015-03-11 | 2015-05-27 | 重庆第二师范学院 | Modification preparation method of maize germ oil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070105204A1 (en) * | 2005-11-10 | 2007-05-10 | Archer-Daniels-Midland Company | Methods for producing propylene glycol monoesters using a lipase |
-
2011
- 2011-04-01 CN CN 201110081424 patent/CN102210355A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070105204A1 (en) * | 2005-11-10 | 2007-05-10 | Archer-Daniels-Midland Company | Methods for producing propylene glycol monoesters using a lipase |
Non-Patent Citations (4)
| Title |
|---|
| 《Bioresource Technology》 20100523 Tarık Öztürk et al Production of medium-chain triacylglycerols from corn oil: Optimization by response surface methodology 7456-7461 1-5 , 第101期 * |
| 《JAOCS》 20040630 In-Hwan Kim et al Production of Structured Lipids by Lipase-Catalyzed Acidolysis in Supercritical Carbon Dioxide: Effect on Acyl Migration 537-541 1-5 第81卷, 第6期 * |
| 《食品科学》 20031231 别小妹 等 脂肪酶催化结构酯的合成及其应用概况 221-225 1-5 第24卷, 第8期 * |
| 《食品科学》 20081231 丁双 等 酶法改良大豆油制备质构脂质的研究 173-176 1-5 第29卷, 第1期 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492743A (en) * | 2011-11-28 | 2012-06-13 | 浙江工业大学 | Method for preparing medium-chain triglyceride from ionic liquid |
| CN102492743B (en) * | 2011-11-28 | 2013-11-13 | 浙江工业大学 | Method for preparing medium-chain triglyceride from ionic liquid |
| CN104642570A (en) * | 2015-03-11 | 2015-05-27 | 重庆第二师范学院 | Modification preparation method of maize germ oil |
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Application publication date: 20111012 |