CN102965402A - Method for preparing diglyceride through utilizing camphor tree seed oil - Google Patents
Method for preparing diglyceride through utilizing camphor tree seed oil Download PDFInfo
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- CN102965402A CN102965402A CN2012104461949A CN201210446194A CN102965402A CN 102965402 A CN102965402 A CN 102965402A CN 2012104461949 A CN2012104461949 A CN 2012104461949A CN 201210446194 A CN201210446194 A CN 201210446194A CN 102965402 A CN102965402 A CN 102965402A
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- seed oil
- camphor tree
- tree seed
- diglyceride
- lipase
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Abstract
A method for preparing diglyceride through utilizing camphor tree seed oil is characterized by comprising the following specific steps that (1) the camphor tree seed oil is put in a temperature control reactor of 45 DEG C-75 DEG C, and LipozymeRMIM or TLIM lipase which accounts for 4%-16% of the mass of the camphor tree seed oil and distilled water which accounts for 20%-60% of the mass of the lipase are added; (2) the rotating speed is regulated to 150-250 rounds/minute to react for 1-4 hours; and (3) after the reaction, the lipase is filtered out, and vacuum dewatering and drying are carried out for 12 hours-24 hours so as to obtain diglyceride. The method takes the camphor tree seed oil as a reaction substrate and has the advantages of simplicity in technology, low production cost, low reaction temperature, short reaction time and high production efficiency, and the diglyceride yield can reach 60%; and moreover, the separation of glyceryl monoaliphatic ester and diglyceride is easier than the separation of diglyceride and triglyceride, so that convenience is brought to diglyceride purification, and industrial production is benefited.
Description
Technical field
The invention belongs to the grease manufacture field.Relate to the triglyceride preparation method.
Background technology
Camphor tree is China's endemic tree, mainly is distributed in areas to the south, the Yangtze valley.The camphor tree seed production is large, oil length high (reaching 60-70%), and the medium-chain fatty acid that lacks the most take China is as main, wherein capric acid content reaches 55%, lauric acid content reaches 38%, both content reach more than 90%, are the high quality raw material of carbochain grease in the complex functionality, have important value of exploiting and utilizing.At present camphor tree seed oil is lacked rationally and utilize, if utilize its production to be rich in the triglyceride of medium-chain fatty acid, will have a good application prospect.
Triglyceride (DAG) is that a lipid acid be the trace ingredients of natural plant grease and the endogenous intermediate product of body fat metabolism, but the content in natural grease is lower by the structured lipid that hydroxyl replaces in the quasi-glycerol three-ester (TAG).DAG has 1,2(2, and 3)-DAG and two kinds of isomerss of 1,3-DAG, owing to be GRAS(Generally Recognized as Safe, usually be considered to safe) material, be used as edible oil in Japan and the U.S..The conventional oil that is rich in DAG at aspects such as taste, outward appearance, physical properties and functions all with the TAG oil phase seemingly.In human body, the consumption of DAG fats oxidn is obviously fast than TAG, and edible DAG oil can reduce blood plasma TAG level and suppress body fat and pile up, and edible DAG oil can reduce obesity for a long time, is conducive to fat-reducing, so also be used as the additive of food and medicine.
At present the enzyme process method for preparing DAG has three kinds of esterification, glycerolysis reaction and hydrolysis reaction.Esterification generates DAG by free fatty acids and glycerine reaction, and the purity that obtains DAG is high, but required free fatty acids is expensive.Glycerolysis reaction is removed TAG acyl group or is passed through Tegin 55G acidylate in reaction, but is difficult to avoid the cohesion of enzyme granulate in the reaction process, causes reacting insufficient.
Summary of the invention
The camphor tree seed oil that the objective of the invention is to take full advantage of China's abundant and be not used effectively is raw material, obtains the camphor tree seed oil triglyceride by lipase-catalyzed hydrolysis.
The present invention includes following concrete steps:
(1) camphor tree seed oil is placed 45 ℃～75 ℃ temperature control reactor, add 4%～16% Lipozyme RM IM or TL IM lipase of camphor tree seed oil quality, and 20%～60% distilled water of enzyme quality.
(2) adjusting rotary speed is to 150-250 r/min reaction 1～4 h.
(3) filter lipase after reaction finishes, dry 12 h of vacuum hydro-extraction～24 h obtain triglyceride.
Principal feature of the present invention: camphor tree seed oil is rich in medium chain fatty acid (〉 90%), medium chain fatty acid has the Absorption And Metabolism characteristics of Quick energizing quantity.Triglyceride is considered to safe material usually, and its oxidation consumption is obviously fast than triglyceride level, and the edible glycerol diester oil can significantly reduce the plasma triglyceride level, suppresses the body fat accumulation and reduce fat.Take camphor tree seed oil as raw material, the triglyceride of middle carbochain is rich in preparation, will be widely used in obesity or the too much patient's of body fat the food.
The invention has the beneficial effects as follows.
1, China's Camphor resources is abundant, and camphor tree seed oil content high (reaching 60-70%), replaces carbochain triglyceride in Oleum Cocois and the plam oil production with camphor tree seed oil, both can increase new resources, can reduce production costs again.
2, hydrolysis reaction is adopted in this test, temperature low (55-75 ℃), and the reaction times short (1-4h), both guaranteed that grease was not destroyed, can enhance productivity again.
3, this test is take camphor tree seed oil as reaction substrate, technique is simple, and the triglyceride productive rate can reach 60%, and because separating of mono-glycerides and triglyceride is easier than separating of triglyceride and triglyceride level, this facilitates for the purification of glycerol diester, more is conducive to suitability for industrialized production.
Specific embodiments
Below in conjunction with specific examples, further set forth the present invention.These examples only are not used in for explanation the present invention and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is usually according to normal condition.Except in addition explanation, otherwise all percentage ratio, ratio and ratio all calculate according to weight.
Except in addition definition, employed all specialty and scientific words and same meanings well-known to those skilled in the art in the literary composition.In addition, any method similar or impartial to described content and material all can be used among the present invention.Better implementation method described in the literary composition and material are only done the usefulness of demonstration.
Embodiment 1.
The camphor tree seed oil that takes by weighing 10g places the temperature control reactor, add immobilized lipase Lipozyme RM IM(oil quality 4% ~ 16%) and distilled water (enzyme quality 40%), put into 65 ℃ on water bath with thermostatic control vibrator and react 3 h, rotating speed is 200 r/min.After reaction finished, qualitative filter paper removed by filter the immobilized enzyme in the reaction solution, and dry 18 h of vacuum hydro-extraction get finished product, and the content of DAG is 30.93%～51.65%(w/w) in the product, and is rich in medium chain fatty acid (60%～90%).
The lipase-catalyzed camphor tree seed oil hydrolysis of table 1:Lipozyme RM IM generates DAG content
| Enzyme concentration (%) | 4 | 6 | 8 | 10 | 12 | 14 | 16 |
| DAG content (%) | 30.93 | 35.33 | 41.32 | 50.19 | 51.65 | 51.65 | 51.65 |
Embodiment 2.
The camphor tree seed oil that takes by weighing 10g places the temperature control reactor, add immobilized lipase Lipozyme RM IM(oil quality 10%) and distilled water (enzyme quality 10% ~ 70%), put into 65 ℃ on water bath with thermostatic control vibrator and react 3 h, rotating speed is 200 r/min.After reaction finished, qualitative filter paper removed by filter the immobilized enzyme in the reaction solution, and dry 18 h of vacuum hydro-extraction get finished product, and the content of DAG is 33.53%～53.09%(w/w) in the product, and is rich in medium chain fatty acid (60%～90%).
The lipase-catalyzed camphor tree seed oil hydrolysis of table 2:Lipozyme RM IM generates DAG content
| Water-content (%) | 10 | 20 | 30 | 40 | 50 | 60 | 70 |
| DAG content (%) | 33.53 | 38.93 | 45.12 | 53.09 | 52.35 | 49.85 | 48.75 |
Embodiment 3.
The camphor tree seed oil that takes by weighing 10g places the temperature control reactor, add immobilized lipase Lipozyme RM IM(oil quality 10%) and distilled water (enzyme quality 40%), put into 45 ℃～75 ℃ reactions of water bath with thermostatic control vibrator, 3 h, rotating speed is 200 r/min.After reaction finished, qualitative filter paper was removed the immobilized enzyme in the reaction solution, and dry 18 h of vacuum hydro-extraction get finished product, and the content of DAG is 41.62%～52.38%(w/w) in the product, and is rich in medium chain fatty acid (60%～90%).
The lipase-catalyzed camphor tree seed oil hydrolysis of table 3:Lipozyme RM IM generates DAG content
| Temperature (℃) | 45 | 55 | 65 | 75 |
| DAG content (%) | 41.62 | 48.57 | 52.38 | 45.23 |
Embodiment 4.
The camphor tree seed oil that takes by weighing 10g places the temperature control reactor, add immobilized lipase Lipozyme RM IM(oil quality 10%) and distilled water (enzyme quality 40%), put into 65 ℃ on water bath with thermostatic control vibrator and react 1 h～4 h, rotating speed is 200 r/min.After reaction finished, qualitative filter paper removed by filter the immobilized enzyme in the reaction solution, and dry 18 h of vacuum hydro-extraction get finished product, and the content of DAG is 20.81%～53.39%(w/w) in the product, and is rich in medium chain fatty acid (60%～90%).
The lipase-catalyzed camphor tree seed oil hydrolysis of table 3:Lipozyme RM IM generates DAG content
| Time (h) | 1 | 2 | 3 | 4 |
| DAG content (%) | 20.81 | 36.78 | 53.45 | 53.39 |
Claims (1)
1. method of utilizing camphor tree seed oil to prepare triglyceride is characterized in that comprising following concrete steps:
(1) camphor tree seed oil is placed 45 ℃～75 ℃ temperature control reactor, add 4%～16% Lipozyme RM IM or TL IM lipase of camphor tree seed oil quality, and 20%～60% distilled water of enzyme quality;
(2) adjusting rotary speed is to 150-250 r/min reaction 1～4 h;
(3) filter lipase after reaction finishes, dry 12 h of vacuum hydro-extraction～24 h obtain triglyceride.
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| CN2012104461949A CN102965402A (en) | 2012-11-09 | 2012-11-09 | Method for preparing diglyceride through utilizing camphor tree seed oil |
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| CN2012104461949A CN102965402A (en) | 2012-11-09 | 2012-11-09 | Method for preparing diglyceride through utilizing camphor tree seed oil |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103352067A (en) * | 2013-08-04 | 2013-10-16 | 中国农业科学院油料作物研究所 | Method for preparing functional grease rich in phytosterol ester and diglyceride |
| CN105463034A (en) * | 2014-07-10 | 2016-04-06 | 丰益(上海)生物技术研发中心有限公司 | Diacylglycerol-rich grease preparation method |
| CN106615239A (en) * | 2016-12-30 | 2017-05-10 | 天津百利食品有限公司 | Preparation method of multifunctional cooking oil |
| CN107058413A (en) * | 2017-04-10 | 2017-08-18 | 南昌大学 | The method of Long carbon chain triglycerides in prepared by a kind of utilization camphor tree seeds oil and soybean oil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802967A (en) * | 2006-01-24 | 2006-07-19 | 浙江大学 | Diglyceride edible oil production method |
-
2012
- 2012-11-09 CN CN2012104461949A patent/CN102965402A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802967A (en) * | 2006-01-24 | 2006-07-19 | 浙江大学 | Diglyceride edible oil production method |
Non-Patent Citations (1)
| Title |
|---|
| 洪鲲等: "响应面法优化固相化脂酶Lipozyme TL IM 催化菜籽油水解过程", 《中国油脂》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103352067A (en) * | 2013-08-04 | 2013-10-16 | 中国农业科学院油料作物研究所 | Method for preparing functional grease rich in phytosterol ester and diglyceride |
| WO2015018206A1 (en) * | 2013-08-04 | 2015-02-12 | 中国农业科学院油料作物研究所 | Method for preparing functional grease rich in phytosterol ester and diglyceride |
| CN105463034A (en) * | 2014-07-10 | 2016-04-06 | 丰益(上海)生物技术研发中心有限公司 | Diacylglycerol-rich grease preparation method |
| CN105463034B (en) * | 2014-07-10 | 2019-08-16 | 丰益(上海)生物技术研发中心有限公司 | A method of preparing the grease rich in diglyceride |
| CN106615239A (en) * | 2016-12-30 | 2017-05-10 | 天津百利食品有限公司 | Preparation method of multifunctional cooking oil |
| CN107058413A (en) * | 2017-04-10 | 2017-08-18 | 南昌大学 | The method of Long carbon chain triglycerides in prepared by a kind of utilization camphor tree seeds oil and soybean oil |
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Application publication date: 20130313 |