CN102210257A - Method for producing seeds of triple cross cotton - Google Patents
Method for producing seeds of triple cross cotton Download PDFInfo
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- CN102210257A CN102210257A CN2010101447885A CN201010144788A CN102210257A CN 102210257 A CN102210257 A CN 102210257A CN 2010101447885 A CN2010101447885 A CN 2010101447885A CN 201010144788 A CN201010144788 A CN 201010144788A CN 102210257 A CN102210257 A CN 102210257A
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Abstract
The invention relates to a method for producing seeds of triple cross cotton. The method comprises the following steps of: cultivating a sterile line of zero type and I type fruit branches, namely hybridizing an upland cotton sterile line with a sea land mixed variety maintaining line to cultivate the sterile line of the zero type and I type fruit branches, namely the female parent; cultivating a restoring line, namely hybridizing an upland cotton variety restoring line with a sea land mixed variety to cultivate a sea land mixed restoring line, namely the male parent; and producing the seeds, namely hybridizing the sterile line of the zero type and I type fruit branches serving as the female parent with the sea land mixed restoring line serving as the male parent to produce the triple cross seeds. By the method, the seed production time is shortened from 40 days to 7 to 10 days; and the yield of the prepared hybrid seeds is increased by 20 percent compared with that of the conventional seeds. The sterility and the sterile strain ratio of the sterile line reach 100 percent; the restoring degree of the hybrid seeds reaches over 95 percent; and the restoring strain ratio reaches 100 percent. The prepared hybrid cotton seeds maintain the advantages of the upland cotton and the high greensickness resistance characteristic of sea island cotton.
Description
Technical field
The present invention relates to agricultural technology field, be specifically related to a kind of producing method for seed of three-line cotton.
Background technology
Traditional cotton hybrid production of hybrid seeds need be removed the maternal stamen of cotton, and the pollen of gathering male parent then carries out artificial pollination.Though the first-filial generation of producing has the advantage on the output, this method recruitment is many, and the production of hybrid seeds time is long, and purity is difficult for guaranteeing.The cotton three series seeding technique, be that male sterile line, recovery are, maintenance line three series mating seeding technique is taken the lead in conducting a research by the U.S. the earliest, the researcher utilizes the male sterile line with Haake west Buddhist nun's cotton cell matter, is to hybridize to produce the hybridization cotton seed with having the recovery that recovers sterile ability.But, go so can't be applied directly in the production because it is poor to recover the recovery capability of system.Effort by China scientist in recent years, the three series mating level of recovering system improved constantly, as Zhejiang University utilize glutathione-S-transferase (GST) thus gene imports Haake west Buddhist nun's cytoplasm recovers to improve in the system recovery capability; The Chinese Academy of Agricultural Sciences molecular biosciences research center transforms the anti insect gene of bt gfm cryla, bt cryla+cpti male sterile line and recovers system.The existing three series mating seeding technique of China can be saved the labour about 60% at present, and the kind of three series mating has begun to use aborning.Breeding cost is higher, cotton hybrid generation output increase rate is not so good as problems such as two-series hybrid but present three-line cotton also exists.
Summary of the invention
The object of the present invention is to provide a kind of producing method for seed of three-line cotton, this producing method for seed has that maternal male sterile line is suitable for dense planting, the high concentration of blooming, can further reduce production of hybrid seeds cost of labor and this first-filial generation output advantages of higher.
In order to realize described purpose, the know-why that the present invention adopts is: adopt zero formula, the I formula fruit branch producing method for seed as the three-line cotton of maternal plant type, it is to hybridize to produce the hybridization cotton seed that mixed recovery of male sterile line and extra large land mixed in the extra large land of the zero formula that is about to select, I formula fruit branch, utilize zero formula, concentrated characteristic of I formula fruit branch cotton phase, the production of hybrid seeds time was foreshortened to 7～10 days from 40 days, reach the purpose that reduces the cotton seed production labour by reduction production of hybrid seeds time and the maternal density of increase.
The invention discloses a kind of producing method for seed of three-line cotton, comprise the following steps:
A) male sterile line of cultivation zero formula, I formula fruit branch: utilize upland cotton male sterile line and extra large land to mix maintenance line and backcross, cultivate the extra large land of zero formula, I formula fruit branch at last and mix male sterile line, be i.e. female parent;
B) cultivate recovery system: it is and the mixed inter breed crossing in extra large land to cultivate the land of going to sea and mix recovery system, i.e. male parent that the upland cotton kind is recovered;
C) production of hybrid seeds: with the sterile of zero formula, I formula fruit branch is that mixed recovery the in female parent, extra large land is paternal hybrid, produces three line hybrid seed.
In a preferred embodiment, described upland cotton is sterile to be to utilize cotton No. 12 hybridization of cotton No. 3 male sterile lines of nasal mucus and Anhui, and wherein Anhui be male parent cotton No. 12, and backcrosses as backcross parent with it and to breed Anhui cotton 12A male sterile line.
In a preferred embodiment, it is to utilize new sea 14 of sea-island cotton or America sea-island cotton and upland cotton chicken foot leaf Anhui 14 to hybridize repeatedly, backcross, are choosing that the kind maintenance line is mixed in described extra large land, cultivates the land of going to sea at last and mixes maintenance line 152 or 134.
The maintenance line seed selection requires: the one, and it is above and the knot bell is strong that fruit branch type zero formula, I formula fruit branch, bell weigh 5 grams; The 2nd, fiber quality suede is long, and clothing part is more than 40% more than 30 millimeters, and more than strong 32cn/Tex, mic value is below 4.2; The 3rd, fusarium wilt refers to that below 10, verticillium wilt refers to below 30.Hybridization between the upland cotton is difficult to reach above breeding goal to maintenance line.The method that adopts extra large land to mix is carried out the seed selection of target type, can reach the maintenance line blow-of-cottons of blooming and concentrate, connects characteristics such as bell is strong, clothing part is high, fiber quality good, anti-withered anti-Huang.Because the filial generation of sea-island cotton and upland cotton can produce serious separation the on strain shape, leaf, the fiber quality, common upland cotton or sea-island cotton be conventional, and to plant seed selection generally stable by reaching proterties about 8 generations.And kind is mixed in extra large land since separate serious, need just can select about 30 generations have objective trait conventional variety as maintenance line.The present invention adopts land, self-fertile sea to mix kind 152 and 134 as maintenance line.This is to hybridize repeatedly, backcross, be choosing with the new sea 14 of sea-island cotton, America sea-island cotton and upland cotton chicken foot leaf Anhui 14, cultivates the land of going to sea at last and mixes kind 152 maintenance lines and 134 maintenance lines.These two strains all have the quality better of sea-island cotton, and the knot bell is strong, the characteristic of anti-withered anti-Huang; Big, the characteristics such as clothing part is high, high-temp resisting high-humidity resisting of the bell that also has upland cotton simultaneously.152 is broad-leaved, and blade is bigger, zero formula, I formula fruit branch: bell weighs 5.6 grams, clothing part 40%, and long 30.9 millimeters of suede, mic value 4.0 is than strong 32.9CN/TEX, 120 days breeding times.134 is chicken foot leaf: bell weighs 7.0 grams, clothing part 42.5, and long 33 millimeters of suede, mic value 3.83, than strong 35.3cn/tex, 122 days breeding times.
In a preferred embodiment, described zero formula, the sterile of I formula fruit branch are 152A male sterile line or 134A male sterile line.
In a preferred embodiment, described recovery is to recover system and cotton 14 hybridization in autonomous seed selection chicken foot leaf Anhui cotton No. 3 with nasal mucus, and selecting that chicken foot leaf Anhui 14 recovers is R9, and mixing with R9 and the sea of containing the BT gene is 116 to be hybridization, and cultivating the land of going to sea, to mix recovery be R2.
Zero formula, I formula fruit branch cotton are as follows as the advantage of male sterile line female parent:
1. it is concentrated that zero formula, I formula fruit branch cotton bloom, the florescence has only 20 days, and the pollination production of hybrid seeds time only needs 7～10 days, and the cotton florescence of general unlimited fruit branch is taken 60 days, the production of hybrid seeds phase is taken 45 days, and every mu of two-series hybrid production of hybrid seeds recruitment needs about 110 workers, and common three line for hybrid seed production needs about 40 workers.The three line for hybrid seed production method of employing zero formula, I formula fruit branch cotton can be so that the recruitment cost be reduced to 10～12 workers.Reduced breeding cost significantly.Calculate through us, 0.07 hectare needs 2～3 people of recruitment to pollinate every morning.The pollination phase is 7～10 days, and human cost is 1/3rd of a common three line for hybrid seed production.
2. zero formula, this proterties of I formula fruit branch are a kind of recessive genes, after the cotton hybridization of loose type fruit branch, Dan Lingchong obviously surpasses male parent and maternal mean value.Zero formula, the cotton conduct female parent of I formula fruit branch, the bell of the made first generation of hybrid heavily has heterobeltiosis, the coordinate force height.
3. three-line parent all is the method seed selection success by sea-land hybridization repeatedly, the characteristic that has kept the Gao Kanghuang of upland cotton advantage and sea-island cotton to wither among the first-filial generation F1, and the region of suitable planting is wide in Cotton Production.
The present invention has following beneficial effect compared with the prior art:
This utilization zero formula, I formula fruit branch as the three-line cotton hybrid method of female parent can save 90% artificial, the conventional kind amount of increase in production of Pei Zhi hybrid can reach 20% simultaneously.Male sterile line sterile degree of the present invention, sterile plant rate all reach 100%, and the crossbreed restoring degree reaches more than 95%, recover the strain rate and reach 100%.152 male sterile line bells weigh 5.6 grams, and it is that bell weighs 5 grams that R2 recovers, and the F1 bell weighs 5.8 grams.
Specific embodiment
Below in conjunction with specific embodiment, further illustrate the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
1. select production of hybrid seeds base.Because zero formula, I formula fruit branch male sterile line are bloomed concentrated, pollination time concentrates in 7～10 days of florescence, it is that pollen breaks that the precipitation in the morning can cause recovery, cause pollination failures, continuous precipitation more can directly have influence on the output of the production of hybrid seeds, therefore the suitable addressing in production of hybrid seeds base annual rainfall 30 millimeter, illumination resource good area such as Kuerle, Xinjiang, Dunhuang, Gansu is to help the quality that the production of hybrid seeds phase avoids precipitation, improves seed.
2. pollination.5～6 on fruit branch is stayed in maternal (152A male sterile line) every mu of about 10000 strains of plantation, and the back of blooming begins pollination an about week, and every morning is awarded Da Hua, 7～10 days pollination phases.
3. sowing maternal (152A male sterile line).Maternal (152A male sterile line) divides the sowing of two phases, and first aspire to before and after April 25, and the second phase was than first phase late sowing 10～15 days.
4. sow male parent (it is R2 that recovery is mixed in extra large land).Sow when male parent (it is R2 that recovery is mixed in extra large land) was sowed in maternal (152A male sterile line) first phase, male parent is not pinched, final singling density 1.2～1.5 ten thousand strain/mus, and pollen can be for maternal use of two phases, and this area of father and mother is 1: 16～1: 20.
Zero formula, I formula three-line cotton production experiment have been done in beginning in 2008 in the Kuerle, Xinjiang, and obtain following good actual effect.
1. breediness
This kind is that the flower medium variety is mixed in extra large land, is combined as 152A * R2, and about 125 days breeding times, the flower rate is more than 95% before the frost; The plant turriform, plant type is more loose, plant height 70～80cm, II-III formula fruit branch, first fruit branch joint position 6.2, the joint position is moderate, fat chicken foot leaf, ventilation and penetrating light is good, the bell oval, median size, bell 4-5 chamber, the bell shell is thin, all bell weighs 5.5g, and boll per plant 6-8, the knot bell is concentrated, and blow-of-cottons are smooth, easily harvesting.The seed pyriform, canescence, son refers to 12.2g, ginning outturn 40-41%, the whole growing growing way is stronger.
2. output
2008-2009 participates in experiment station's varietal yield test, and real receipts of unginned cotton is respectively every mu of 550kg and 578kg before the two annual frosts, and comparison is according to Anhui 14 volume increase 18% and 20% respectively; Performance stable high yield characteristic.
3. prematurity
Zero formula, I formula three-line cotton time of infertility is 125 days, and comparison was according to cotton 14 precocities in Anhui 1～2 day in 2008-2009 experiment station variety comparative test, and the flower rate is respectively 94.5%～99.5% before the frost, and prematurity is outstanding.
4. fiber quality
Detect through cotton quality supervision and inspection center of the Ministry of Agriculture: the on average long 33.9mm of fiber, mic value 4.3, specific strength 33.26cN/tex, the good requirement that meets textile industry centering long-staple cotton physical index of fiber quality.
5. disease resistance
Identified and cutd open the stalk evaluation by 81 experiment stations of agricultural eight teachers in the plantation of blight garden in 2009, zero formula, I formula triple crossing fusarium wilt of cotton strain rate are 15.8%, and disease refers to 5.7; Verticillium wilt strain rate is 20.8%, and disease refers to be 18.9; Belong to anti-withered anti-verticillium wilt kind.
Below be 152 three-line cotton F1 output application forms.
| Time | Plant height cm | First fruit branch joint position joint | Fruit branch number | Big bell/strain | Boll per plant/strain | Actual density strain/mu | Total bell several/mu |
| 2008 | 68 | 6.2 | 8.2 | 8.0 | 8.8 | 11500 | 101200 |
| 2009 | 70 | 6.2 | 8.1 | 8.2 | 8.4 | 12500 | 105000 |
Scope of the present invention is not subjected to the restriction of described specific embodiments, and described embodiment also comprises the method and the component of functional equivalent only as the single example of illustrating various aspects of the present invention in the scope of the invention.In fact, except content as herein described, those skilled in the art can easily grasp multiple improvement of the present invention with reference to above description and accompanying drawing.Described improvement also falls within the scope of appended claims.Every piece of list of references mentioned above is listed this paper in as a reference all in full.
Claims (8)
1. the producing method for seed of a three-line cotton comprises the following steps:
A) male sterile line of cultivation zero formula, I formula fruit branch: utilize upland cotton male sterile line and extra large land to mix maintenance line and backcross, cultivate the extra large land of zero formula, I formula fruit branch at last and mix male sterile line, be i.e. female parent;
B) cultivate recovery system: it is and the mixed inter breed crossing in extra large land to cultivate the land of going to sea and mix recovery system, i.e. male parent that the upland cotton kind is recovered;
C) production of hybrid seeds: with the sterile of zero formula, I formula fruit branch is that mixed recovery the in female parent, extra large land is paternal hybrid, produces three line hybrid seed.
2. producing method for seed as claimed in claim 1, it is characterized in that described upland cotton sterile be to utilize cotton No. 12 hybridization of cotton No. 3 male sterile lines of nasal mucus and Anhui, wherein Anhui be male parent cotton No. 12, and backcrosses as backcross parent with it and to breed Anhui cotton 12A male sterile line.
3. producing method for seed as claimed in claim 1 is characterized in that it is to utilize new sea 14 of sea-island cotton or America sea-island cotton and upland cotton chicken foot leaf Anhui 14 to hybridize repeatedly, backcross, are choosing that maintenance line is mixed in described extra large land, cultivates the land of going to sea at last and mixes maintenance line 152 or 134.
4. producing method for seed as claimed in claim 1 is characterized in that the sterile 152A of the being male sterile line or the 134A male sterile line of described zero formula, I formula fruit branch.
5. producing method for seed as claimed in claim 1; it is characterized in that described recovery is to recover system and cotton 14 hybridization in autonomous seed selection chicken foot leaf Anhui cotton No. 3 with nasal mucus; selecting that chicken foot leaf Anhui 14 recovers is R9, and mixing with R9 and the sea of containing the BT gene is 116 to be hybridization, and cultivating the land of going to sea, to mix recovery be R2.
6. producing method for seed as claimed in claim 1 is characterized in that every mu of about 10000 strains of plantation of described female parent, stays 5～6 on fruit branch, and the back of blooming begins pollination an about week, and every morning is awarded Da Hua, 7～10 days pollination phases.
7. producing method for seed as claimed in claim 1 is characterized in that the described maternal two phases sowing that divides, and first aspire to before and after April 20, and the second phase was than first phase late sowing 10～15 days.
8. producing method for seed as claimed in claim 1 is characterized in that sowing when institute's male parent that is set forth in was sowed in the maternal first phase, and male parent is not pinched, final singling density 1.2～1.5 ten thousand strain/mus, and pollen can be for maternal use of two phases, and this area of father and mother is 1: 16～1: 20.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101447885A CN102210257A (en) | 2010-04-09 | 2010-04-09 | Method for producing seeds of triple cross cotton |
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| CN2010101447885A CN102210257A (en) | 2010-04-09 | 2010-04-09 | Method for producing seeds of triple cross cotton |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1103542A (en) * | 1993-12-09 | 1995-06-14 | 王余军 | Breeding method of high-yield variety of cotton with several bolls in one node |
| CN1586162A (en) * | 2004-08-31 | 2005-03-02 | 北京科欣基业科技发展有限公司 | Cultivating method for super early mature short season cotton |
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2010
- 2010-04-09 CN CN2010101447885A patent/CN102210257A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1103542A (en) * | 1993-12-09 | 1995-06-14 | 王余军 | Breeding method of high-yield variety of cotton with several bolls in one node |
| CN1586162A (en) * | 2004-08-31 | 2005-03-02 | 北京科欣基业科技发展有限公司 | Cultivating method for super early mature short season cotton |
Non-Patent Citations (2)
| Title |
|---|
| 杨亚东: "陆海三系杂交棉的配制及应用研究", 《新疆农垦科技》 * |
| 杨仁碧: "陆海三系杂交棉制种技术", 《新疆农业科学》 * |
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Application publication date: 20111012 |