CN102199532A

CN102199532A - Cell phototoxicity experimental detection device

Info

Publication number: CN102199532A
Application number: CN2011100926444A
Authority: CN
Inventors: 范维林
Original assignee: Tianjin Hope Industry and Trade Co Ltd
Current assignee: Tianjin Hope Industry and Trade Co Ltd
Priority date: 2011-04-14
Filing date: 2011-04-14
Publication date: 2011-09-28

Abstract

The invention provides a cell phototoxicity experimental detection device. The cell phototoxicity experimental detection device comprises a lightproof experimental chamber, wherein the top of the lightproof experimental chamber is provided with a plurality of ultraviolet radiation light sources which can emit 365nm of ultraviolet A; the inside of the experimental chamber is provided with a plurality of cell culture solution cavities which can be fixed by cell diaphragm plates, an upper ultraviolet quartz window protects the cell diaphragm plates to be isolated from the outside and ensures that the cell diaphragm plates are radiated by the ultraviolet radiation light sources; an incubation cavity is connected with an incubation controller and a cell culture solution supply device which are required for the survival of cells; the inside of the experimental chamber is provided with an ultraviolet and ozone disinfector, a ventilation device and a humidity and temperature controller to ensure that the experimental environment meets requirements; an radiation light probe detects the intensity of the UVA ultraviolet radiation light sources and is controlled by a computer; and the experimental parameters can be stored to the connected computer or printed by a printer. The invention has the following beneficial effects: the invention provides the cell phototoxicity experimental detection device and the device can be widely used in the cell phototoxicity scientific researches of the fields such as chemical industry, hygienic toxicology, environmental friendliness, labor occuplational disease prevention and medical science.

Description

Cell phototoxicity experiment test set

Technical field

The present invention relates to a kind of cell phototoxicity experiment test set.

Background technology

In the daily life, though many medicines and cosmetics of everyday use can not cause direct injury to human body, but under action of ultraviolet ray, for example operate under sun exposure, the ultraviolet environments etc. under the condition chemical reaction just can take place, thereby cause skin allergy or other forms of injury, be referred to as the phototoxicity injury that chemical has on the specialty.This type of chemical preparations is carried out the phototoxicity test experience can prevent and alleviate the disadvantageous effect that this class phototoxicity is brought to us.Existing phototoxicity identification experiment does not have the equipment of specialty, and used light source is impure, and directly uses laboratory animal to shine, and causes experimental result inaccurate, and conventional efficient is low, and experiment condition is abominable, and operator are dangerous.Be badly in need of a kind of experimental precision, safety, convenience, efficiently, test test set can accurately controlled at above problem, replenish blank in this respect without the cell phototoxicity of laboratory animal.

Summary of the invention

For addressing the above problem; the purpose of this invention is to provide a kind of cell phototoxicity experiment test set; for relevant research experiment provides a kind of experimental precision, safety, convenience, efficiently, test test set without the cell phototoxicity of laboratory animal can accurately controlled; for providing new method and apparatus, replenished blank in this respect at field research experiments in this regard such as chemical industry, toxicity health, environment protection, work prophylaxis, medical science.

For achieving the above object, technical scheme of the present invention provides a kind of cell phototoxicity experiment test set, this equipment comprises lighttight Laboratory Module body, and having several can send single wavelength at Laboratory Module body inner top is that the ultraviolet lamp of UVA uv irradiation light source of 365nm is as experimental light sources; Several cell cultures sap cavities are arranged in it, the cell lamina membranacea can be fixed, accepting wavelength is the irradiation of the UVA ultraviolet source of 365nm; Being close to ultraviolet quartz window protection cell lamina membranacea above the cell cultures sap cavity is isolated from the outside and accepts the uv irradiation light source irradiation; The incubation chamber connects required incubation controller and the cell culture fluid supply of cells survival; Ultraviolet and ozonateur, ventilation plant and temperature and humidity controller are arranged in the Laboratory Module, experimental situation is met the requirements; With the exposure light probe of the same level attitude of cell lamina membranacea the UVA uv irradiation intensity of light source is detected and controls by computer; Parameters such as the process of experiment, result can be stored in the computer or by printer and get the laboratory report list.

Effect of the present invention is: provide a kind of can accurately control experimental precision, safety, convenience, efficiently, without the cell phototoxicity experiment test set of laboratory animal; for providing new method and apparatus, replenished blank in this respect at field research experiments in this regard such as chemical industry, toxicity health, environment protection, work prophylaxis, medical science.

Description of drawings

Accompanying drawing is the synoptic diagram of structure of the present invention;

Among the figure:

1, ultraviolet disinfecting

2, UVA uv irradiation light source

3, ozonateur

4, ultraviolet quartz window

5, cell lamina membranacea

6, cell cultures sap cavity

7, cell culture fluid supply

8, exposure light probe

9, incubation chamber

10, incubation controller

11, ultraviolet and ozonateur time delay switch

12, exposure light test set

13, ventilation plant

14, temperature and humidity controller

15, time controller

16, computer

17, printer

18, Laboratory Module.

Embodiment

Reach embodiment in conjunction with the accompanying drawings the present invention be illustrated,

As shown in drawings, present device include comprise lighttight Laboratory Module 18, its top have several can send single wavelength be 365nm UVA uv irradiation light source 2 ultraviolet lamp as the experiment radiation source; Several cell cultures sap cavities 6 are arranged in it, can cell lamina membranacea 5 is fixing, top ultraviolet quartz window 4 protection cell lamina membranaceas 5 are isolated from the outside and accept 2 irradiations of UVA uv irradiation light source; Incubation chamber 9 connects required incubation controller 10 and the cell culture fluid supply 7 of cells survival; Ultraviolet disinfecting 1 and ozonateur 3, ventilation plant 13 and temperature and humidity controller 14 by ultraviolet and 11 controls of ozonateur time delay switch are arranged in the Laboratory Module 18, experimental situation is met the requirements, time controller 15 control experimental periods; The uv irradiation light intensity has exposure light test set 12 to monitor by the exposure light probe 8 that is in same level attitude with cell lamina membranacea 5, and controls by computer 16; Experimentation can be stored in computer 16 interior or printer 17 print processing that are connected with parameter.

Embodiment

Using present device to carry out at first opening ultraviolet disinfecting 1 when the phototoxicity experiment detects and ozonateur 3 carries out the sufficiently sterilised sterilization to each cell cultures sap cavity 6, open incubation controller 10 simultaneously, close ultraviolet and ozonateur time delay switch 11 after the sterilization and also open the hermatic door and the ultraviolet quartz window 4 of Laboratory Module 18 immediately, the cell lamina membranacea of having handled in advance 5 is placed in the cell cultures sap cavity 6,18 of closed ultraviolet quartz window 4 and Laboratory Modules, open cell culture fluid supply 7, open ventilation plant 13 and temperature and humidity controller 14 makes experimental situation reach requirement, opening time controller 15 and irradiation flash ranging device 12 monitoring irradiation time and intensity, the ultraviolet lamp of opening wavelength and be the UVA uv irradiation light source 2 of 365nm begins cell phototoxicity test experience, experiment is omnidistance can be by the outside computer 16 at-once monitor adjustment that connect, and experiment parameter can be stored in the computer 16 that is connected or by printer 17 print processing.Computer 16 can cut out the ultraviolet lamp that wavelength is the UVA uv irradiation light source 2 of 365nm automatically after experiment was finished; The experimenter take out experimental cell lamina membranacea 5 by relevant regulation and standard carry out this kind chemical substance to the evaluation of skin reaction intensity to draw this experimental result.

Claims

1. cell phototoxicity experiment test set, it is characterized in that: this equipment comprises lighttight Laboratory Module (18) body, having several can send single wavelength at Laboratory Module (18) body inner top is that the UVA uv irradiation light source (2) of 365nm is as experimental light sources; Several cell cultures sap cavities (6) are arranged in Laboratory Module (18) body, can fixed cell lamina membranacea (5), on cell cultures sap cavity (6), there is ultraviolet quartz window (4) protection cell shuttering (5) to be isolated from the outside and accepts UVA uv irradiation light source (2) irradiation that wavelength is 365nm; Is that the experiment light intensity of the UVA uv irradiation light source (2) of 365nm detects and controlled testing processes such as light intensity and experiment condition by computer (16) with cell lamina membranacea (5) par position exposure light probe (8) being arranged to single wavelength; Inner ultraviolet disinfecting (1), the ozonateur (3) of Laboratory Module body (18) guarantees to test to be operated under aseptic condition; Ventilation plant (13) and temperature and humidity controller (14) guarantee that the intravital environment of Laboratory Module (18) meets requirement of experiment; Parameters such as the process of experiment, result can be stored in the computer (16) or by printer (17) and print the laboratory report list.

2. a kind of cell phototoxicity experiment test set according to claim 1, it is characterized in that: lighttight Laboratory Module (18) body, it is that the ultraviolet lamp of the UVA uv irradiation light source (2) of 365nm seals that the dodge gate that adopts light screening material and sealing can send single wavelength with several, and the ultraviolet lamp of UVA uv irradiation light source (2) is installed in Laboratory Module (18) the body inner top directly over the cell lamina membranacea (5).

3. a kind of cell phototoxicity experiment test set according to claim 1, it is characterized in that: several cell cultures sap cavities (6) are arranged in Laboratory Module (18) body, a plurality of cell lamina membranaceas (5) can be fixed in the cell cultures sap cavity (6), connecting cell culture fluid supply (7) in the cell cultures sap cavity (6) provides nutrition at any time the cell lamina membranacea (5) in the cell cultures sap cavity (6), cell cultures sap cavity (6) and cell lamina membranacea (5) are in same incubation chamber (9), and incubation chamber (9) connect incubation controller (10) and guarantee cell cultures sap cavity (6) and cell lamina membranacea (5) and the inner essential temperature of experimental cell existence.

4. a kind of cell phototoxicity experiment test set according to claim 1 is characterized in that: Laboratory Module (18) body inner close fitting cell cultures sap cavity (6) and its interior cell lamina membranacea (5) to be had ultraviolet quartz window (4) can protect cell shuttering (5) to be isolated from the outside above just and accepts the irradiation that wavelength is the UVA uv irradiation light source (2) of 365nm.

5. a kind of cell phototoxicity experiment test set according to claim 1, it is characterized in that: the inner position of Laboratory Module (18) with cell lamina membranacea (5) equal height be provided with incubation chamber (9) in the isolated exposure light probe of liquid phase (8), be that the experiment light intensity of UVA uv irradiation light source (2) irradiation of 365nm detects and calculates and whole experiment is controlled testing parameters such as light intensity and experimental period by computer (16) to single wavelength.