CN103018244A - Rapid phototoxicity experiment equipment with simultaneous exposure of multiple point positions - Google Patents
Rapid phototoxicity experiment equipment with simultaneous exposure of multiple point positions Download PDFInfo
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- CN103018244A CN103018244A CN2012104851827A CN201210485182A CN103018244A CN 103018244 A CN103018244 A CN 103018244A CN 2012104851827 A CN2012104851827 A CN 2012104851827A CN 201210485182 A CN201210485182 A CN 201210485182A CN 103018244 A CN103018244 A CN 103018244A
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Abstract
The invention provides rapid phototoxicity experiment equipment with simultaneous exposure of multiple point positions. The equipment comprises a light-absorbing surface operating console, a main control instrument plate with a camera, and a light path box supported by an altitude controller, wherein experimental animals are placed in the middle of the light-absorbing surface operating console; a UVA (ultraviolet A) powerful light source of the light path box adopts high-voltage strong-light halide lamp as a UVA pure light source, an experimental region and an experimental area of the experimental animals are adjustable, and laser is used for locating and position calibration. In an experiment, a foot pedal control circuit is stepped so as to start the UVA powerful light source in the light path box, and under the feedback of a UVA light intensity measurer and the control of the control circuit, the UVA powerful light source is switched on and appropriately delayed, so that enough UVA light rays simultaneously and electrically irradiate on multiple points of back skins of the experimental animals to implement a rapid phototoxicity exposure test. The equipment provided by the invention has the beneficial effects that the UVA powerful light source is adopted for implementing the exposure and irradiation of the multiple point positions simultaneously, so that the speed is high, and the consistency is good; the localization of the experimental animals is exact, experimental data are accurate, and the pollution caused by operating staff can be avoided; and the experimental equipment can be widely applied to scientific researches in the fields of chemical industry, labor and occupational protection, medicines and the like.
Description
Technical field
The invention provides a kind of multi-point and expose simultaneously fast light poison experimental facilities.
Background technology
After phototoxicity refers to the skin contact chemical substance, then be exposed to the lower a kind of Skin toxicity that causes of ultraviolet ray irradiation, the phototoxicity experiment is that a certain amount of tested material is spread upon on the plucked skin of back part of animal, through being exposed to the experiment of simulated solar irradiation under the UVA light of wavelength in the 320-400 nanometer range behind the certain hour interval, whether skin occurs thick and heavy after the UVA light irradiation of observation animal subject process doses, red and swollen, macula, the bad reaction such as bubble also determines that according to the Comparison of standards of relevant regulations thereby whether this tested material has phototoxicity for animal and reason out for human possibility and cause the phototoxicity injury.Light poison experimental facilities in the past is to use common UV light source or pointolite, because the light intensity of common UV light source is lower, the experiment irradiation time is longer, light source can produce thermal effect, thereby the irradiation field temperature is raise, animal is felt under the weather and the movement that strains at the leass, and the position of irradiation also affects experimental result thereupon mobile dislocation, and therefore experiment operator can touch ultraviolet ray and come to harm; The pointolite light intensity is high but can only the single-point irradiation, although irradiation time is short, it is low to need multiple spot to shine one by one long-acting rate of overall time during experiment, the bad constraint of animal, so a kind of multi-point of development and Design exposes simultaneously fast light poison equipment and becomes the task of top priority.
Summary of the invention
For addressing the above problem, the purpose of this invention is to provide a kind of multi-point and expose simultaneously the fast light poison in fact
Test equipment, according to the formula of reconditioning:
Annotate: 1 mw/cm
2=1 mJ/cm
2/ sec
Reckoning bring up to the light intensity of UVA intense light source large or when equaling 10000mW irradiation time just can shorten to little or equal 1 second, can use the millisecond meter evaluation time, this kind is with under the millisecond meter evaluation time length situation, short speed of the time that light exposes is fast, animal used as test is controlled well, the exposure intensity high conformity of multi-point, the animal used as test accurate positioning, experimental data is accurate, and effectively the fence operation personnel are subject to light pollution.
For achieving the above object, the technical solution used in the present invention is that this equipment comprises an extinction face operator's console that is placed on the support, animal used as test can be placed in its middle, be positioned at the operator and on one side main control instrument plate with camera be installed the front, position, another side is fixed with a height adjuster that supports the light path box, it is positioned at animal used as test top, extinction face operator's console center and can adjusts up and down height, main control instrument plate is provided with control circuit and operation keyboard and display, control circuit is by being electrically connected UVA luminous intensity measurement device and its inner UVA intense light source of installing on the control light path box, the UVA intense light source is a special employing high voltage high light gold Halogen lamp LED, its surface adopts quartz material can the high temperature resistant while not have loss for the light of UVA wavelength, adhere to last layer in the surface vacuum coating method of lamp and contain the special light transmissive material of gold to guarantee that the high light that sees through is the single light source of UVA wavelength, special HT stabilizer provides power supply by the control of main control instrument plate to high voltage high light gold Halogen lamp LED, the only high stable that assurance is sent, high dose luminosity, the pure light source of UVA of monochromatic wavelength, the different parts to animal used as test imposes identical in short time to reach, the light poison experiment of sufficient exposure dose irradiation, thereby realize the experiment of fast light poison.Also have the laser positioning point can proofread and correct the accurate location that animal used as test is laid on the light path box, light path box side is equipped with between left and right can penetrate the A district by regulating illumination apart from adjusting knob and fore-and-aft clearance adjusting knob, irradiation B district, irradiation C district, before and after between the irradiation D district, about horizontal range, light path box bottom is equipped with a plurality of light conducting cylinders and will be transmitted out simultaneously by UVA light filtration focusing and the multiple spot that the UVA intense light source is launched, the foot-switch that is positioned at extinction face operator's console bottom is electrically connected to control circuit, and the inner UVA intense light source of installing of control circuit start light path box is opened under UVA luminous intensity measurement device feedback and control circuit control and suitable time-delay UVA intense light source is radiated at animal used as test back skin with the UVA light multiple spot while electricity of sufficient dosage and carries out the phototoxicity exposure test when being operated.
The invention has the beneficial effects as follows that having developed a kind of multi-point exposes fast light poison experimental facilities simultaneously, multi-point exposes irradiation simultaneously in the experiment of light poison, indifference between the each point, data are accurate, the fast efficient of speed is high, because the irradiation time weak point does not have temperature rise and sluggish in air circulationly causes spinoff, can take back animal used as test locates and just steps on foot-switch and finish exposure at once, need not fetter animal used as test, therefore very applicable to zoopery in enormous quantities, spacing and the area of light exposure test block are adjustable, be applicable to large toy, laser positioning is accurate, has effectively protected operating personnel and has been polluted, and can be widely used in chemical industry, the work prophylaxis, the scientific research in the fields such as medical science.
Description of drawings
Accompanying drawing 1 exposes the structural representation of fast light poison experimental facilities simultaneously for a kind of multi-point of present device
Accompanying drawing 2 is experiment irradiation area and laser positioning vertical view
Among the figure
1, between left and right apart from adjusting knob 2, fore-and-aft clearance adjusting knob 3, interference filter 4, collector lens
5, control panel extinction face 6, foot-switch 7, main control instrument plate 8, camera
9, light conducting cylinder 10, movable shadow shield 11, animal used as test 12, extinction face operator's console
13, UVA luminous intensity measurement device 14, laser positioning point 15, height adjuster 16, golden Halogen lamp LED
17, UVA intense light source 18, circular reflection shield 19, light path box 20, irradiation D district
21, irradiation B district 22, irradiation A district 23, irradiation C district
Embodiment
By reference to the accompanying drawings 1 and 2 and embodiment a kind of multi-point of the present invention exposed fast light poison experimental facilities simultaneously be illustrated.
Shown in attached Fig. 1 and 2, the invention provides a kind of multi-point and expose simultaneously fast light poison experimental facilities, this equipment comprises an extinction face operator's console 12 that is placed on the support, be one stably rectangle operating table surface of stent support is arranged, upper surface is smooth smooth and to scribble light absorbent not reflective, can place animal used as test 11, at middle part, long limit height adjuster 15 is installed and supports light path box 19, it is positioned at extinction face operator's console animal used as test 11 tops, 12 center and can adjusts up and down height, be positioned at operator another middle part, long limit on one side main control instrument plate 7 is installed, the operator can expose experiment in its back operation.Main control instrument plate 7 is provided with control circuit and operation keyboard and display, control circuit is by being electrically connected UVA luminous intensity measurement device 13 and its inner UVA intense light source 17 of installing on the control light path box 19, main control instrument plate 7 is rectangular thin type boxes, be erected on extinction face operator's console 12 one side, its size and highly just in time can the isolated operation person and the irradiation of the UVA intense light source 17 of light path box 19, by the control panel extinction face 5 at the back side in the operator's console be made by light absorbent not reflective, the top is equipped with camera 8 and shoots with video-corder in real time animal used as test 11, there are supervisory keyboard and display in front facing to the operator, inside has circuit controller to be electrically connected control UVA luminous intensity measurement device 13, UVA intense light source 17 and foot-switch 6 step on when placing animal used as test 11 that foot-switch 6 triggers and control UVA intense light source 17 irradiation times and image and experimental data be presented on the display.Light path box 19 is fixed on the unsettled top, position, extinction face operator's console 12 center that is positioned on the crossbeam of height adjuster 15, can according to the size height of animal used as test 11 by regulate knob on the height adjuster 15 just can up-down adjustment its highly, light path box 19 is the airtight box bodys that lighttight exotic material is made, UVA intense light source 17 of fixed installation in the middle of airtight box body inside, inwall of back-off is coated with the circular reflection shield 18 of special optical reflectance coating directly over the UVA intense light source 17, a plurality of below light path box 19 of the emergency light reflex of the UVA wavelength that UVA intense light source 17 is sent can arbitrarily adjust on the light conducting cylinder 9 of spacing and outwards transmit, UVA intense light source 17 is special employing high voltage high light gold Halogen lamp LEDs 16, its surface adopts quartz material can the high temperature resistant while not have loss for the light of UVA wavelength, adhere to last layer in the surface vacuum coating method of lamp and contain the special light transmissive material of gold to guarantee that the high light that sees through is the single light source of UVA wavelength, special HT stabilizer provides power supply by the control of main control instrument plate 7 to high voltage high light gold Halogen lamp LED, the only high stable that assurance is sent, high dose luminosity, the pure light source of UVA of monochromatic wavelength, the different parts to animal used as test 11 imposes identical in the short time to reach, the light poison experiment of sufficient exposure dose irradiation.Light path box 19 bottoms are equipped with a plurality of light conducting cylinders 9 and will be transmitted out simultaneously by UVA light filtration focusing and the multiple spot that UVA intense light source 17 is launched, light conducting cylinder 9 is cylinders of Long Circle two printing opacity, interference filter 3 and the collector lens 4 that can only see through the UVA wavelength is equipped with in its inside, top is connecting to be had between left and right apart from adjusting knob 1 and fore-and-aft clearance adjusting knob 2, and the bottom is equipped with movable shadow shield 10 and guarantees that light conducting cylinder 9 not only can but also not have leakage of light out in the following random horizontal adjustment position of light path box 19.Light path box 19 sides be equipped be connected to light conducting cylinder 9 tops between left and right apart from adjusting knob 1 and fore-and-aft clearance adjusting knob 2, can move horizontally to adapt to the horizontal range of all around between irradiation A district 22, irradiation B district 21, irradiation C district 23, the irradiation D district 20 according to the size adjustment light conducting cylinder 9 of animal used as test 11.Also have laser positioning point 14 can proofread and correct the accurate location that animal used as test 11 is laid on the light path box 19, laser positioning point 14 is LASER Light Source that are fixed on light path box bottom, adjust it and send the middle that the minor diameter hot spot just in time is radiated at it irradiation A district 22 of animal used as test 11, irradiation B district 21, irradiation C district 23, irradiation D district 20, so that whether accurately proofreaded the position that animal used as test 11 lays under the condition of not opening UVA intense light source 17 correct.UVA luminous intensity measurement device 13 is one and is contained in the following special light intensity inductor of light path box 19 by support, can open instantaneous measurement in UVA intense light source 17 and go out to shine light intensity, by the continuity irradiation time after being installed in control circuits in the main control instrument plate 7 and automatically controlling UVA intense light source 17 and open, to reach the dosage of accurate control animal used as test 11 irradiated light intensities.The foot-switch 6 that is positioned at extinction face operator's console 12 bottoms is electrically connected to control circuit, and control circuit start light path box 19 inner UVA intense light sources 17 of installing are opened under UVA luminous intensity measurement device 13 feedbacks and control circuit control and suitable time-delay UVA intense light source 17 is radiated at animal used as test 11 back skins with the UVA light multiple spot while electricity of sufficient dosage and carries out the phototoxicity exposure test when being operated.
Embodiment:
Use a kind of multi-point of the present invention to expose simultaneously and should at first be ready to animal used as test 11 and tested material before fast light poison experimental facilities is tested, according to the relevant standard code of skin Phototoxicity experiment before carrying out the experiment of light poison 18-24 hour, with animal backbone both sides skin unhairing, the test position needs intact, harmless injuring unusually, go out to prepare out 4 unhairing districts at animal used as test back, every unhairing area is about 2 * 2cm, then in irradiation A district 22 and irradiation B district 21, smear tested material, after 30 minutes, cover irradiation B district 21 and irradiation D district 20 with aluminium foil, ready animal used as test 11 is placed on the extinction face operator's console 12, according to the height of animal subject 11 height by the knob up-down adjustment light path box 19 on the adjusting height adjuster 15, according to size adjustment light conducting cylinder 9 tops of animal used as test 11 between left and right apart from adjusting knob 1 and fore-and-aft clearance adjusting knob 2, regulate light conducting cylinder 9 and move horizontally to adapt to irradiation A district 22, irradiation B district 21, irradiation C district 23, before and after between the irradiation D district 20, about horizontal range, connecting device power supply (DPS) locates with laser positioning point 14, laser positioning point 14 is LASER Light Source that are fixed on light path box bottom, adjusting it sends the minor diameter hot spot and just in time is radiated at animal used as test 11 irradiation A district 22 with it, irradiation B district 21, irradiation C district 23, the middle in irradiation D district 20, accurately proofreaded the correct position that animal used as test 11 is laid, control circuit start light path box 19 inner UVA intense light sources 17 of installing are opened under UVA luminous intensity measurement device 13 feedbacks and control circuit control and suitable time-delay UVA intense light source 17 is radiated at animal used as test 11 back skins with the UVA light multiple spot while fast electric of sufficient dosage and carries out the phototoxicity exposure test when stepping on foot-switch 6, be presented on the display on the main control instrument plate 7 simultaneously and with image and experimental data, test complete, take off animal subject 11, all finish all animals used as test if the experiment of carrying out in enormous quantities can repeat aforesaid operations rapidly and close the rear closing device power supply of exposure, finishing light exposes rear respectively at 1,24,48, observe dermoreaction after 72 hours, whether occur thick and heavy according to skin, red and swollen, macula, the bad reaction such as bubble also determines that according to the Comparison of standards of relevant regulations thereby whether this tested material has phototoxicity for animal used as test and reason out for human possibility and cause the phototoxicity injury.
Claims (8)
1. a multi-point exposes fast light poison experimental facilities simultaneously, it is characterized in that: this equipment comprises an extinction face operator's console (12) that is placed on the support, animal used as test (11) can be placed in its middle, be positioned at the operator and on one side main control instrument plate (7) with camera (8) be installed the front, position, another side is fixed with a height adjuster (15) that supports light path box (19), it is positioned at extinction face operator's console (12) center animal used as test (11) top and can adjusts up and down height, main control instrument plate (7) is provided with control circuit and operation keyboard and display, control circuit is by being electrically connected UVA luminous intensity measurement device (13) and its inner UVA intense light source (17) of installing on the control light path box (19), light path box (19) is upper to also have laser positioning point (14) can proofread and correct the accurate location that animal used as test (11) is laid, light path box (19) side is equipped with between left and right can penetrate A district (22) by regulating illumination apart from adjusting knob (1) and fore-and-aft clearance adjusting knob (2), irradiation B district (21), irradiation C district (23), before and after between the irradiation D district (20), about horizontal range, light path box (19) bottom is equipped with a plurality of light conducting cylinders (9) and will be transmitted out simultaneously by UVA light filtration focusing and the multiple spot that launch UVA intense light source (17), be positioned at the following foot-switch (6) of extinction face operator's console (12) and be electrically connected to control circuit, the inner UVA intense light source (17) of installing of control circuit start light path box (19) is radiated at animal used as test (11) back skin with the UVA light multiple spot while electricity of sufficient dosage and carries out the phototoxicity exposure test in the lower suitable time-delay UVA intense light source (17) also opened of UVA luminous intensity measurement device (13) feedback and control circuit control when being operated.
2. a kind of multi-point according to claim 1 exposes fast light poison experimental facilities simultaneously, it is characterized in that: described extinction face operator's console (12) is one stably rectangle operating table surface of stent support, upper surface is smooth smooth and to scribble light absorbent not reflective, can place animal used as test (11), at middle part, long limit height adjuster (15) is installed and supports light path box (19), another middle part, long limit is equipped with main control instrument plate (7), and the operator can carry out light in its back operation and expose experiment.
3. a kind of multi-point according to claim 1 exposes fast light poison experimental facilities simultaneously, it is characterized in that: described main control instrument plate (7) is a rectangular thin type box, be erected on extinction face operator's console (12) one side, its size and highly just in time can the isolated operation person and the irradiation of the UVA intense light source (17) of light path box (19), by the control panel extinction face (5) at the back side in the operator's console be made by light absorbent not reflective, the top is equipped with camera (8) and shoots with video-corder in real time animal used as test (11), there are supervisory keyboard and display in front facing to the operator, inside has circuit controller to be electrically connected control UVA luminous intensity measurement device (13), UVA intense light source (17) and foot-switch (6) step on when placing animal used as test (11) that foot-switch (6) triggers and control UVA intense light source (17) irradiation time and image and experimental data be presented on the display.
4. a kind of multi-point according to claim 1 exposes fast light poison experimental facilities simultaneously, it is characterized in that: described light path box (19) is an airtight box body that lighttight exotic material is made, a fixed installation UVA intense light source (17) in the middle of airtight box body inside, inwall of back-off is coated with the circular reflection shield (18) of special optical reflectance coating directly over the UVA intense light source (17), a plurality of below light path box (19) of the emergency light reflex of the UVA wavelength that UVA intense light source (17) is sent can adjust arbitrarily that the light conducting cylinders (9) of spacing are upper outwards to be transmitted, light path box (19) is fixed on the unsettled top, position, extinction face operator's console (12) center that is positioned on the crossbeam of height adjuster (15), can according to the size height of animal used as test (11) by regulate knob on the height adjuster (15) just can up-down adjustment its highly, light path box (19) side be equipped be connected to light conducting cylinder (9) top between left and right apart from adjusting knob (1) and fore-and-aft clearance adjusting knob (2), can move horizontally to adapt to irradiation A district (22) according to the size adjustment light conducting cylinder (9) of animal used as test (11), irradiation B district (21), irradiation C district (23), before and after between the irradiation D district (20), about horizontal range.
5. a kind of multi-point according to claim 1 exposes fast light poison experimental facilities simultaneously, it is characterized in that: described UVA intense light source (17) is a special employing high voltage high light gold Halogen lamp LED, its surface adopts quartz material can the high temperature resistant while not have loss for the light of UVA wavelength, adhere to last layer in the surface vacuum coating method of lamp and contain the special light transmissive material of gold to guarantee that the high light that sees through is the single light source of UVA wavelength, special HT stabilizer provides power supply by the control of main control instrument plate (7) to high voltage high light gold Halogen lamp LED, the only high stable that assurance is sent, high dose luminosity, the pure light source of UVA of monochromatic wavelength, the different parts to animal used as test (11) imposes identical in the short time to reach, the light poison experiment of sufficient exposure dose irradiation.
6. a kind of multi-point according to claim 1 exposes fast light poison experimental facilities simultaneously, it is characterized in that: described light conducting cylinder (9) is the cylinder of Long Circle two printing opacity, interference filter (3) and the collector lens (4) that can only see through the UVA wavelength is equipped with in its inside, top is connecting to be had between left and right apart from adjusting knob (1) and fore-and-aft clearance adjusting knob (2), and the bottom is equipped with movable shadow shield (10) and guarantees that light conducting cylinder (9) not only can but also not have leakage of light out in the following random horizontal adjustment position of light path box (19).
7. a kind of multi-point according to claim 1 exposes fast light poison experimental facilities simultaneously, it is characterized in that: described UVA luminous intensity measurement device (13) is one and is contained in the following special light intensity inductor of light path box (19) by support, can open instantaneous measurement in UVA intense light source (17) and go out to shine light intensity, automatically control continuity irradiation time after UVA intense light source (17) is opened by being installed in control circuit in the main control instrument plate (7), to reach the dosage of the irradiated light intensity of accurate control animal used as test (11).
8. a kind of multi-point according to claim 1 exposes fast light poison experimental facilities simultaneously, it is characterized in that: described laser positioning point (14) is a LASER Light Source that is fixed on light path box bottom, adjust it and send the middle that the minor diameter hot spot just in time is radiated at it irradiation A district (22) of animal used as test (11), irradiation B district (21), irradiation C district (23), irradiation D district (20), so that whether accurately proofreaded the position that animal used as test (11) lays under the condition of not opening UVA intense light source (17) correct.
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| CN201210485182.7A CN103018244B (en) | 2012-11-26 | 2012-11-26 | Rapid phototoxicity experiment equipment with simultaneous exposure of multiple point positions |
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| CN201210485182.7A CN103018244B (en) | 2012-11-26 | 2012-11-26 | Rapid phototoxicity experiment equipment with simultaneous exposure of multiple point positions |
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| CN103018244B CN103018244B (en) | 2014-11-26 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103323455A (en) * | 2013-04-24 | 2013-09-25 | 南京文采科技有限责任公司 | Tobacco leaf grading method based on reflection, perspective and microscopic images |
| CN106190797A (en) * | 2016-07-23 | 2016-12-07 | 吉林建筑大学 | A kind of device for microalgae phototaxis research |
| CN107917887A (en) * | 2017-12-18 | 2018-04-17 | 天津开发区合普工贸有限公司 | A kind of quick tester of conveyor type animal phototoxicity |
| CN111787847A (en) * | 2018-02-26 | 2020-10-16 | 株式会社爱茉莉太平洋 | Skin change measuring device and method using blue light, and blue light irradiation device |
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| US20040009091A1 (en) * | 2002-03-15 | 2004-01-15 | Deal Jeffery L. | C-band disinfector |
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| CN103323455A (en) * | 2013-04-24 | 2013-09-25 | 南京文采科技有限责任公司 | Tobacco leaf grading method based on reflection, perspective and microscopic images |
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| CN111787847A (en) * | 2018-02-26 | 2020-10-16 | 株式会社爱茉莉太平洋 | Skin change measuring device and method using blue light, and blue light irradiation device |
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