CN202022937U

CN202022937U - Detection device of cell phototoxicity experiments

Info

Publication number: CN202022937U
Application number: CN 201120107898
Authority: CN
Inventors: 范维林
Original assignee: Tianjin Hope Industry and Trade Co Ltd
Current assignee: Tianjin Hope Industry and Trade Co Ltd
Priority date: 2011-04-14
Filing date: 2011-04-14
Publication date: 2011-11-02
Anticipated expiration: 2021-04-14

Abstract

The utility model provides a detection device of cell phototoxicity experiments, which comprises a lightproof experimental chamber. The top of the experimental chamber is provided with a plurality of ultraviolet A (UVA) irradiation light sources with single wavelength as 365nm. A plurality of cell culture solution cavities capable of fixing membrane plates are arranged in the experimental chamber, and an ultraviolet quartz window protects the membrane plates from the outside environment and enables the membrane plates to be lighted by the UVA irradiation light sources. A warm culture chamber is connected with a warm culture controller and a cell culture solution supply device which are required by cells for life. An ultraviolet and ozone sterilizer, a ventilating device and a humiture controller are arranged in the experimental chamber to enable the experimental environment to meet the requirement. An irradiation light probe detects the intensity of the UVA irradiation light sources and is controlled by a computer, and experimental parameters can be stored in the connected computer or printed through a printer. The detection device has the advantage of being widely used for scientific research on phototoxicity in the fields of chemistry industry, toxicological health, environmental protection, labor and job danger prevention, medical science and the like.

Description

Cell phototoxicity experiment proofing unit

Technical field

The utility model relates to a kind of cell phototoxicity experiment proofing unit.

Background technology

In the daily life, though many medicines and cosmetics of everyday use can not cause direct injury to human body, but under action of ultraviolet ray, just chemical reaction can take place under the operational condition under sun exposure, the ultraviolet environments for example, thereby cause skin allergy or other forms of injury, be referred to as the phototoxicity injury that chemical has on the specialty.This type of chemical preparations is carried out the phototoxicity test experience can prevent and alleviate the disadvantageous effect that this class phototoxicity is brought to us.Existing phototoxicity identification experiment does not have the device of specialty, and used light source is impure, and directly uses laboratory animal to shine, and causes experimental result inaccurate, and conventional efficient is low, and experiment condition is abominable, and operator are dangerous.Be badly in need of a kind of experimental precision, safety, convenience, efficiently, test proofing unit can accurately controlled at above problem, replenish blank in this respect without the cell phototoxicity of laboratory animal.

Summary of the invention

For addressing the above problem; the purpose of this utility model provides a kind of cell phototoxicity experiment proofing unit; for relevant research experiment provides a kind of experimental precision, safety, convenience, efficiently, test proofing unit without the cell phototoxicity of laboratory animal can accurately controlled; for providing new method and apparatus, replenished blank in this respect at chemical industry, toxicity health, environment protection, work prophylaxis, medical field research experiment in this regard.

For achieving the above object, the technical solution of the utility model provides a kind of cell phototoxicity experiment proofing unit, this device comprises lighttight Laboratory Module body, and having several can send single wavelength at Laboratory Module body inner top is that the ultraviolet lamp of UVA uv irradiation light source of 365nm is as experimental light sources; Several cell cultures sap cavities are arranged in it, the cell lamina membranacea can be fixed, accepting wavelength is the irradiation of the UVA ultraviolet source of 365nm; Being close to ultraviolet quartz window protection cell lamina membranacea above the cell cultures sap cavity is isolated from the outside and accepts the uv irradiation light source irradiation; The incubation chamber connects required incubation controller and the cell culture fluid supply of cells survival; Ultraviolet and ozonateur, ventilation plant and temperature and humidity controller are arranged in the Laboratory Module, experimental situation is met the requirements; With the exposure light probe of the same level attitude of cell lamina membranacea the UVA uv irradiation intensity of light source is detected and controls by computer; Process, the result parameter of experiment can be stored in the computer or by printer and get the laboratory report list.

Effect of the present utility model is: provide a kind of can accurately control experimental precision, safety, convenience, efficiently, without the cell phototoxicity experiment proofing unit of laboratory animal; for providing new method and apparatus, replenished blank in this respect at chemical industry, toxicity health, environment protection, work prophylaxis, medical field research experiment in this regard.

Description of drawings

Accompanying drawing is the synoptic diagram of the utility model structure;

Among the figure:

1, ultraviolet disinfecting

2, UVA uv irradiation light source

3, ozonateur

4, ultraviolet quartz window

5, cell lamina membranacea

6, cell cultures sap cavity

7, cell culture fluid supply

8, exposure light probe

9, incubation chamber

10, incubation controller

11, ultraviolet and ozonateur time delay switch

12, exposure light test set

13, ventilation plant

14, temperature and humidity controller

15, time controller

16, computer

17, printer

18, Laboratory Module

Embodiment

Reaching embodiment in conjunction with the accompanying drawings is illustrated the utility model.

As shown in drawings, the utility model device includes lighttight Laboratory Module 18, being provided with several at cabin body inner top, to send single wavelength be that the UVA uv irradiation light source 2 of 365 nm is as experimental light sources, Laboratory Module 18 is made by light screening material, the cabin body is provided with Packed dodge gate the ultraviolet lamp that several are installed in the UVA uv irradiation light source 2 of cabin body inner top is sealed, guarantee that UVA uv irradiation light can't see through the Laboratory Module body, the ultraviolet lamp of UVA uv irradiation light source 2 be installed in cell lamina membranacea 5 directly over cabin body inner top; Several cell cultures sap cavities 6 are arranged in Laboratory Module 18 bodies, a plurality of cell lamina membranaceas 5 can be fixed on cell cultivates in the sap cavity 6, each cell cultures sap cavity 6 all is connected to cell culture fluid supply 7 and nutrition is provided at any time the cell lamina membranacea 5 of cell cultures sap cavity 6 inside, the cell lamina membranacea 5 of each cell cultures sap cavity 6 and Qi Nei is in same incubation chamber 9, and incubation chamber 9 guarantees cell cultures sap cavity and cell lamina membranacea 5 and the inner essential temperature of experimental cell existence by connecting incubation controller 10; Be close to cell cultures sap cavity 6 and its inner cell lamina membranacea 5 just above be provided with ultraviolet quartz window 4, can protect cell lamina membranacea 5 to be isolated from the outside and accept UVA uv irradiation light source 2 irradiations that wavelength is 365nm; Level attitudes inner at Laboratory Module 18 and cell lamina membranacea 5 equal heights be provided with incubation chamber 9 in the isolated exposure light probe 8 of liquid phase, be that the experiment light intensity that the UVA uv irradiation light source 2 of 365nm shines detects and controls by exposure light test set 12 connection computers 16 to single wavelength; Have in the Laboratory Module 18 by the ultraviolet disinfecting 1 and the ozonateur 3 assurance experiments of ultraviolet and 11 controls of ozonateur time delay switch and under aseptic condition, operate; Ventilation plant 13 and temperature and humidity controller 14 meet the requirements experimental situation, time controller 15 control experimental periods; Experimentation can be stored in computer 16 interior or printer 17 print processing that are connected with parameter.

Embodiment

Using the utility model device to carry out at first opening ultraviolet disinfecting 1 when the phototoxicity experiment detects and ozonateur 3 carries out the sufficiently sterilised sterilization to each cell cultures sap cavity 6, open incubation controller 10 simultaneously, close ultraviolet and ozonateur time delay switch 11 after the sterilization and also open the hermatic door and the ultraviolet quartz window 4 of Laboratory Module 18 immediately, the cell lamina membranacea of having handled in advance 5 is placed in the cell cultures sap cavity 6,18 of closed ultraviolet quartz window 4 and Laboratory Modules, open cell culture fluid supply 7, open ventilation plant 13 and temperature and humidity controller 14 makes experimental situation reach requirement, opening time controller 15 and irradiation flash ranging device 12 monitoring irradiation time and intensity, the ultraviolet lamp of opening wavelength and be the UVA uv irradiation light source 2 of 365nm begins cell phototoxicity test experience, experiment is omnidistance can be by the outside computer 16 at-once monitor adjustment that connect, and experiment parameter can be stored in the computer 16 that is connected or by printer 17 print processing.Computer 16 can cut out the ultraviolet lamp that wavelength is the UVA uv irradiation light source 2 of 365nm automatically after experiment was finished; The experimenter take out experimental cell lamina membranacea 5 by relevant regulation and standard carry out this kind chemical substance to the evaluation of skin reaction intensity to draw this experimental result.

Claims

1. cell phototoxicity experiment proofing unit, it is characterized in that: this device comprises lighttight Laboratory Module (18) body, having several can send single wavelength at Laboratory Module (18) body inner top is that the UVA uv irradiation light source (2) of 365nm is as experimental light sources; Several cell cultures sap cavities (6) are arranged in Laboratory Module (18) body, can fixed cell lamina membranacea (5), on cell cultures sap cavity (6), there is ultraviolet quartz window (4) protection cell shuttering (5) to be isolated from the outside and accepts UVA uv irradiation light source (2) irradiation that wavelength is 365nm; Is that the experiment light intensity of the UVA uv irradiation light source (2) of 365nm detects and controlled testing light intensity and experiment condition process by computer (16) with cell lamina membranacea (5) par position exposure light probe (8) being arranged to single wavelength; Inner ultraviolet disinfecting (1), the ozonateur (3) of Laboratory Module body (18) guarantees to test to be operated under aseptic condition; Ventilation plant (13) and temperature and humidity controller (14) guarantee that the intravital environment of Laboratory Module (18) meets requirement of experiment; Process, the result parameter of experiment can be stored in the computer (16) or by printer (17) and print the laboratory report list.

2. a kind of cell phototoxicity experiment proofing unit according to claim 1, it is characterized in that: lighttight Laboratory Module (18) body, it is that the ultraviolet lamp of the UVA uv irradiation light source (2) of 365nm seals that the dodge gate that adopts light screening material and sealing can send single wavelength with several, and the ultraviolet lamp of UVA uv irradiation light source (2) is installed in Laboratory Module (18) the body inner top directly over the cell lamina membranacea (5).

3. a kind of cell phototoxicity experiment proofing unit according to claim 1, it is characterized in that: several cell cultures sap cavities (6) are arranged in Laboratory Module (18) body, a plurality of cell lamina membranaceas (5) can be fixed in the cell cultures sap cavity (6), connecting cell culture fluid supply (7) in the cell cultures sap cavity (6) provides nutrition at any time the cell lamina membranacea (5) in the cell cultures sap cavity (6), cell cultures sap cavity (6) and cell lamina membranacea (5) are in same incubation chamber (9), and incubation chamber (9) connect incubation controller (10) and guarantee cell cultures sap cavity (6) and cell lamina membranacea (5) and the inner essential temperature of experimental cell existence.

4. a kind of cell phototoxicity experiment proofing unit according to claim 1 is characterized in that: Laboratory Module (18) body inner close fitting cell cultures sap cavity (6) and its interior cell lamina membranacea (5) to be had ultraviolet quartz window (4) can protect cell shuttering (5) to be isolated from the outside above just and accepts the irradiation that wavelength is the UVA uv irradiation light source (2) of 365nm.

5. a kind of cell phototoxicity experiment proofing unit according to claim 1, it is characterized in that: the inner position of Laboratory Module (18) with cell lamina membranacea (5) equal height be provided with incubation chamber (9) in the isolated exposure light probe of liquid phase (8), be that the experiment light intensity of UVA uv irradiation light source (2) irradiation of 365nm detects and calculates and whole experiment is controlled testing light intensity and experimental period parameter by computer (16) to single wavelength.