CN102191566B - The method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie - Google Patents
The method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie Download PDFInfo
- Publication number
- CN102191566B CN102191566B CN201010119192.XA CN201010119192A CN102191566B CN 102191566 B CN102191566 B CN 102191566B CN 201010119192 A CN201010119192 A CN 201010119192A CN 102191566 B CN102191566 B CN 102191566B
- Authority
- CN
- China
- Prior art keywords
- ramie
- fresh
- fiber crops
- enzyme
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Chemical Or Physical Treatment Of Fibers (AREA)
Abstract
The invention provides the Degumming method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh fiber crops of ramie.The present invention is by alkaline pectase and hemicellulase mixing, and the enzyme preparation obtained can be directly used in the fresh degumming process of ramie, solves the problem that chemical degumming law and biological bacteria degumming method effectively cannot make the fresh retting of ramie; Simultaneously; the fresh degumming process of ramie provided by the invention is simple; the fresh fiber crops preparation of ramie is all carried out with the leaching enzyme step of the fresh fiber crops of ramie in farmland; chemicals consumption in autoclaving treatment fluid is few; and do not use toxic chemical, after obtained ramee, in waste water, chemical oxygen content is low, containing a large amount of phosphorus, potassium, sodium plasma; so waste water can direct irrigated farmland, not only save cost but also protected environment.
Description
Technical field
The present invention relates to China grass degumming field, be specifically related to the method for a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie.
Background technology
Ramie is a kind of time-honored crops originating in China.Containing a large amount of as-spun fibres in its bast, ramie filament length is 40mm ~ 450mm, is the longest in crudefiber crop.Filament fineness 5.0dtex ~ 6.67dtex, cross section is polygonal or ellipse, and good springiness, good insulating, pliability are high, is a kind of textile raw material crops having economic worth.
Ramie just can obtain as-spun fibre after needing to carry out degumming process, and traditional Degumming method is for being immersed in spontaneous fermentation in water by the fresh fiber crops of unseasoned ramie after ramie harvesting, and then washes away colloid, beat again, get rid of to wash and dry, and obtain ramie harl, obtain ramie as-spun fibre; Or fresh for ramie fiber crops are dried and obtains ramie raw ramie, by the colloid in chemical Degumming or biological bacteria or enzymatic degradation ramie raw ramie and lignin, then dry obtain ramie as-spun fibre by sour rinsing, high pressure kiering.
In prior art, first method is the most original ramie degumming method, and usually time is long, process regulation difficulty, and degumming quality is not high, uneven yet, cannot realize large-scale industrial production; And second method only can be used in ramie raw ramie, can not be directly used in the fresh fiber crops of ramie, because chemical oxygen content (COD) and inorganic particulate pollute the environment in the waste water of chemical Degumming, and the ramie as-spun fibre that chemical Degumming obtains is uneven; And use biological bacteria to come unstuck, the time that biological bacteria generates biology enzyme is long, and when utilizing biological bacteria to come unstuck, the small-molecule substance discharged when the metabolite of biological bacteria and generation biology enzyme can cause the rising of COD in factory effluent, and the condition of culture of biological bacteria is harsh, high to operating personnel's technical requirement, implement difficulty in field large, if do not carry out deactivation operation or deactivation improper, very easily cause secondary pollution.
Summary of the invention
The technical problem to be solved in the present invention is, provides the method for a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie, uses described enzyme preparation directly to come unstuck operation to the fresh fiber crops of ramie, simple, power consumption is few, and cost is low, pollutes little.
In order to overcome the above problems, the invention provides the method for the fresh retting of a kind of ramie, comprising:
Green grass or young crops is gone to obtain ramie fresh fiber crops the ramie peeling of having gathered in;
Fresh for described ramie fiber crops are soaked 20h ~ 30h in enzyme preparation;
The fresh fiber crops of ramie after described immersion enzyme preparation are copied fiber crops in advance on flax stamping device;
Copy the fresh fiber crops of the ramie after fiber crops in advance carry out autoclaving by described;
Fresh for ramie after described autoclaving fiber crops are copied fiber crops on flax stamping device, obtains as-spun fibre;
Described as-spun fibre is carried out pickling, washing, and oiling to dry after washing away impurity obtains ramee.
Preferably, described autoclaving specifically comprises:
Copy the fresh fiber crops of the ramie after fiber crops in advance put into water by described, then add NaSiO in water
3, NaOH, auxiliary agent, H
2o
2obtain mixture;
Pressure is standard atmospheric pressure, temperature is heat described mixture 0.5h ~ 1h at 90 DEG C ~ 100 DEG C, then described pressure is increased to 0.2MPa, continues the described mixed liquor 0.5h ~ 2.5h of heating.
Preferably, described auxiliary agent is poly (sodium aspartate).
The fresh retting enzyme preparation of a kind of ramie, comprising:
Enzyme activity is alkaline pectase and the hemicellulase of 50U/mL ~ 5000U/mL.
Preferably, described hemicellulase is at least made up of the one in once biology enzyme: the dextranase that the neutral xylanase that enzyme activity is 500U/mL ~ 2000U/mL, enzyme activity are 4000U/mL ~ 50000U/mL, enzyme activity are the mannase of 600U/mL ~ 50000U/mL.
Preferably, also comprise with enzyme preparation by volume percentage be the surfactant of 0.1% ~ 10% and the chelating agent of 0.1% ~ 10%.
Preferably, described surfactant is at least made up of the one in following nonionic surface active agent: ethoxylated dodecyl alcohol, cetanol polyoxyethylene ether.
Preferably, described chelating agent is the atactic polymer of acrylic acid, acrylate, maleic anhydride.
A preparation method for the fresh retting enzyme preparation of ramie, comprising:
Surfactant and chelating agent being added temperature is in the water of 30 DEG C ~ 50 DEG C, stirs, and dissolves, obtains the first mixed liquor after cooling;
Alkaline pectase, neutral xylanase, dextranase, mannase are added in the first mixed liquor respectively, obtains enzyme preparation.
Preferably, in described enzyme preparation, the enzyme activity of various enzyme is: alkaline pectase 50U/mL ~ 5000U/mL, neutral xylanase 500U/mL ~ 2000U/mL, dextranase 4000U/mL ~ 50000U/mL, mannase 600U/mL ~ 50000U/mL.
The invention provides a kind of method using described enzyme preparation to come unstuck to the fresh fiber crops of ramie, comprising: go green grass or young crops to obtain ramie fresh fiber crops the ramie peeling of having gathered in; Fresh for described ramie fiber crops are soaked 20h ~ 30h in enzyme preparation; The fresh fiber crops of ramie after described immersion enzyme preparation are copied fiber crops in advance on flax stamping device; Copy the fresh fiber crops of the ramie after fiber crops in advance carry out autoclaving by described; Fresh for ramie after described autoclaving fiber crops are copied fiber crops on flax stamping device, obtains as-spun fibre; Described as-spun fibre is carried out pickling, washing, dries after removing impurity and obtain ramee.Describedly come unstuck simple to operate, the chemical reagent dosage used in autoclaving is little, reduces cost, simultaneously because employ enzyme preparation instead of biological bacteria to avoid secondary pollution in scouring processes.The invention provides the fresh retting enzyme preparation of a kind of ramie, comprising: the mannase that the neutral xylanase that the alkaline pectase that enzyme activity is 50U/mL ~ 5000U/mL, enzyme activity are 500U/mL ~ 2000U/mL, enzyme activity are the dextranase of 4000U/mL ~ 50000U/mL, enzyme activity is 600U/mL ~ 50000U/mL and surfactant and chelating agent.The fresh retting enzyme preparation of ramie provided by the invention is owing to being the colloid that the enzymatic directly used decomposes in ramie, and enzyme does not have life, so the change of enzymatic activity can not be caused when using the fresh fiber crops of ramie and cause the ramee quality of production unstable, be directly used in irrigated farmland containing a large amount of phosphorus, nitrogen, potassium etc. in waste water after simultaneously coming unstuck, reduce cost.Present invention also offers a kind of preparation method of described enzyme preparation, comprising: surfactant and chelating agent being added temperature is in the water of 30 DEG C ~ 50 DEG C, stirs, dissolve, after cooling, obtain the first mixed liquor; Alkaline pectase, neutral xylanase, dextranase, mannase are added in the first mixed liquor respectively, obtains enzyme preparation.Described preparation method is simple to operate, facilitates large-scale industrial production.
Detailed description of the invention
In order to understand the present invention further, below in conjunction with embodiment, the preferred embodiments of the invention are described, but should be appreciated that these describe just as further illustrating the features and advantages of the present invention instead of the restriction to patent requirements of the present invention.
Explanation of nouns
The fresh fiber crops of ramie: the definition of fresh fiber crops is ramie raw ramies also not dry after harvesting, comprise only through peeling go the fresh fiber crops of green grass or young crops of the ramie after green grass or young crops and machine combining processed after the ramie raw materials also do not done.
Ramie raw ramie: the definition of raw ramie is that the ramie of drying after green grass or young crops is gone in peeling after harvesting processes raw material, and comprises manual dry fiber crops and the dry fiber crops of machine stripping.
The invention provides the method for the fresh retting of a kind of ramie, comprising: go green grass or young crops to obtain ramie fresh fiber crops the ramie peeling of having gathered in; Fresh for described ramie fiber crops are soaked 20h ~ 30h in enzyme preparation; The fresh fiber crops of ramie after described immersion enzyme preparation are copied fiber crops in advance on flax stamping device; Copy the fresh fiber crops of the ramie after fiber crops in advance carry out autoclaving by described; Fresh for ramie after described autoclaving fiber crops are copied fiber crops on flax stamping device, obtains as-spun fibre.Described as-spun fibre is carried out pickling, washing, and after removing impurity, oiling to dry obtains ramee.
Described autoclaving specifically comprises:
Copy the fresh fiber crops of the ramie after fiber crops in advance put into water by described, then add NaSiO in water
3, NaOH, auxiliary agent, H
2o
2obtain mixed liquor;
Pressure is standard atmospheric pressure, temperature is heat described mixed liquor 0.5h ~ 1h at 90 DEG C ~ 100 DEG C, then described pressure is increased to 0.2MPa, continues the described mixed liquor 0.5h ~ 2.5h of heating.
China grass degumming refers to the process pectin in ramie and lignin degradation or removing being obtained ramee.
According to the present invention, the fresh fiber crops of ramie are ramie raw materials also not dry after ramie harvesting, and the fresh fiber crops of ramie can be that after the manual harvesting of peasant, peeling goes green grass or young crops to obtain, and also can be that after mechanical harvesting, peeling goes green grass or young crops to obtain.Be different from ramie raw ramie, containing large quantity of moisture and liquid organic matter in the fresh fiber crops of ramie, which results in and can cause when using chemical Degumming method to come unstuck to the fresh fiber crops of ramie insufficient problem of coming unstuck, and when using biological bacteria Degumming method to come unstuck to the fresh fiber crops of ramie, due to the harshness of its mushroom condition of culture, large quantity of moisture in the fresh fiber crops of ramie and organic meeting impact the breeding metabolism of bacterium, the efficiency that makes to come unstuck is low, if use biological bacteria Degumming method simultaneously, the improper meeting of mushroom deactivation remains in waste water, once waste water is discharged will cause secondary pollution.And enzyme preparation provided by the invention, pectin in the fresh fiber crops of direct use enzymatic degradation ramie and lignin, do not need to carry out catalysis with biological bacteria generation enzyme again to come unstuck, do like this and can the catalysis of fast and stable come unstuck, reduce costs and turn avoid metabolite that biological bacteria produces in scouring processes to the impact of efficiency of coming unstuck, and the secondary pollution that discharge of wastewater causes.
According to the present invention, be immersed in the container filling enzyme preparation provided by the invention by fresh for the ramie prepared fiber crops, carry out the operation of leaching enzyme, leaching enzyme temperature is preferably 10 DEG C ~ 55 DEG C, is more preferably 30 DEG C ~ 50 DEG C; The leaching enzyme time is preferably 20h ~ 30h, is more preferably 24h ~ 28h; PH value is preferably 7.0 ~ 10.0, is more preferably 8.5 ~ 9.5; Enzyme dosage accounts for the fresh numb total weight percent of ramie and is preferably 1% ~ 4.0%, is more preferably 1.5% ~ 2.5%.The operation of leaching enzyme terminates rear taking-up and is placed on and flax stamping device copies in advance fiber crops operation.The flax stamping device that the present invention uses is preferably the flax stamping device that cast iron manufactures, by support and runner and copy fiber crops hammer and motor form, the fresh fiber crops of ramie after having been operated by leaching enzyme are placed on runner, start motor, under the drive of motor, the fresh fiber crops of ramie are followed runner and rotate, and copy fiber crops hammer simultaneously and ceaselessly beat the fresh fiber crops of ramie, make the internal structure of the fresh fiber crops of ramie loose.Preferably copy fiber crops 2 ~ 3 circle in advance.
According to the present invention, copy in advance fiber crops operation terminate after, preferably will copy in advance the ramie after fiber crops fresh fiber crops dress cage, be preferably the every cage of 1000kg ~ 1500kg, then be placed on boil drift pond in, carry out autoclaving operation.So-called autoclaving be exactly use boil the process of drift treatment fluid copy fiber crops in advance after the fresh fiber crops of ramie, remove pigment, the process of impurity.Add autoclaving treatment fluid to boiling in drift pond, this treatment fluid comprises: quality optimization is the NaSiO for the fresh numb weight 2.5% ~ 3.5% of ramie
3; Add the NaOH that quality optimization is 10kg ~ 20kg; Add the auxiliary agent that quality optimization is the fresh numb weight 0.5% ~ 1.5% of ramie, described auxiliary agent is preferably poly (sodium aspartate); Add the H that quality optimization is the fresh numb weight 1% ~ 2% of ramie
2o
2, and the water of 5000kg ~ 6000kg, obtain mixture.Then heat this mixture at normal atmospheric pressure, temperature is preferably 90 DEG C ~ 100 DEG C, and heat time heating time is preferably 0.5h ~ 1h, then described pressure is increased to 0.2MPa, continues the described mixed liquor 0.5h ~ 2.5h of heating.
According to the present invention .H in autoclaving treatment fluid
2o
2mainly discoloration is played to the as-spun fibre in the fresh fiber crops of ramie, reach achromatic objective by degraded pigment unsaturated bond, also there is boiling-off and go deimpurity effect; NaOH plays digesting agent, main removes cellulose commensal, comprises hemicellulose, pectic substance, lignin, wax, ash grade, also have adjust ph and accelerate H
2o
2the effect of decomposing; Poly (sodium aspartate) has the effects such as good wetting, infiltration, washing, emulsification, dispersion, can improve the wettability of the fresh fiber crops of ramie. be conducive to the infiltration for the treatment of fluid. and impurity is easily washed from fiber and falls; NaSiO
3stabilizing agent. for control H
2o
2decomposition, in treatment fluid, add suitable stabilizing agent effectively and fully can utilize H
2o
2, and reduce the damage to as-spun fibre as far as possible.
After autoclaving process, the pectin in the fresh fiber crops of ramie has all been degraded with lignin etc. or has been separated with as-spun fibre, then places it in and flax stamping device copies fiber crops 3 ~ 5 circle, thoroughly by as-spun fibre and pectin and lignin separation.Obtain as-spun fibre, then by as-spun fibre through overpickling 1 ~ 2 time, wash 1 ~ 2 time, in and alkaline matter residual on as-spun fibre, fully wash away enzyme preparation and depart from the pectin of as-spun fibre and the catabolite of lignin, and various auxiliary agent and undecomposed H
2o
2.Oil after having washed, dry and obtain ramee.
The preparation of the fresh fiber crops of ramie of the present invention and the leaching enzyme step of the fresh fiber crops of ramie are preferably carried out between ramie farmland, reduce factory site and personnel depaly, can expand plant layout like this and reduce costs.
The invention provides the fresh retting enzyme preparation of a kind of ramie to comprise: the mannase that the neutral xylanase that the alkaline pectase that enzyme activity is 50U/mL ~ 5000U/mL, enzyme activity are 500U/mL ~ 2000U/mL, enzyme activity are the dextranase of 4000U/mL ~ 50000U/mL, enzyme activity is 600U/mL ~ 50000U/mL and surfactant and chelating agent.Wherein alkaline pectase belongs to the one of pectase, also has a kind of acid pectase in addition, because enzyme preparation provided by the invention acts on the fresh fiber crops of ramie, and moisture is large in the fresh fiber crops of ramie, if use acid pectase, it will inevitably be hydrolyzed and cause enzyme preparation catalysis degumming effect and the fresh numb quality instability of obtained ramie, so the present invention uses alkaline pectase.The effect of alkaline pectase is that pectin in the fresh fiber crops of catalysis ramie and other colloidal material fast degradations become small-molecule substance, thus can decompose in the process of autoclaving more stable more rapidly, and show that the ramee quality only having enzyme activity to obtain at 50U/mL ~ 5000U/mL could be stablized through the great many of experiments of the present inventor; Be hemicellulase according to neutral xylanase of the present invention, dextranase, mannase, act on hemicellulose and lignin, cellulose is stripped out from the fresh fiber crops of ramie.
According to the present invention, owing to employing alkaline pectase, so preferably use nonionic surface active agent, such as ethoxylated dodecyl alcohol well known in the art, cetanol polyoxyethylene ether.The present invention preferably uses ethoxylated dodecyl alcohol, obtained by laruyl alcohol and ethyleneoxide addition, wherein according to the difference of oxirane number, oleophylic hydrophilic group equilibrium index (HLB) is between 10 ~ 16, this illustrates that ethoxylated dodecyl alcohol can be good at reducing interfacial tension, make the enzyme in enzyme preparation can be good at contacting with between ramie colloid and other materials, add the effect that enzymatic is decomposed.
Also preferably add chelating agent in enzyme preparation of the present invention, described chelating agent preferably uses the random copolymer that in acrylic acid, acrylate, maleic anhydride, two or more is formed, and the degree of polymerization is 100-300.The effect of chelating agent uses the multidentate ligand in chelator frameworks to be fixed the metal ion in the fresh fiber crops of ramie and to extract out by coordination effect, and its many carboxyl structure can effectively disperse the colloid in ramie to improve purity and the intensity of ramee simultaneously.
Present invention also offers a kind of preparation method of described enzyme preparation, comprising: surfactant and chelating agent being added temperature is in the water of 30 DEG C ~ 50 DEG C, stirs, dissolve, after cooling, obtain the first mixed liquor;
Alkaline pectase, neutral xylanase, dextranase, mannase are added in the first mixed liquor respectively, obtains enzyme preparation.
According to the present invention, ethoxylated dodecyl alcohol and chelating agent being added temperature is in 30 DEG C ~ 50 DEG C water, stirs, obtains the first mixed liquor after ethoxylated dodecyl alcohol and chelating agent all dissolve.The temperature of water is preferably 35 DEG C ~ 45 DEG C, and the percent by volume that the amount adding ethoxylated dodecyl alcohol and chelating agent accounts for enzyme preparation total amount is respectively preferably 0.1% ~ 10%, is more preferably 0.5% ~ 2%.Alkaline pectase, neutral xylanase, dextranase, mannase are added in the first mixed liquor, because the activity of various enzyme is different, so the dosage adding enzyme will meet, alkaline pectase enzyme activity is 50U/mL ~ 5000U/mL, the enzyme activity of neutral xylanase is 500U/mL ~ 2000U/mL, the enzyme activity of dextranase is 4000U/mL ~ 50000U/mL, the enzyme activity of mannase is 600U/mL ~ 50000U/mL.Wherein U/mL to represent at 25 DEG C in the 1min that every milliliter of enzyme preparation contains the enzyme amount transformed needed for 1 μm of ol substrate.So also enzyme content can be regarded as.After reaching enzyme activity requirement, stir, be China grass degumming enzyme preparation provided by the invention.
The source of the present invention to alkaline pectase, neutral xylanase, dextranase, mannase does not limit, and is preferably the biology enzyme using biological bacteria fermentation release in advance.
The invention provides a kind of China grass degumming enzyme preparation, the colloid in ramie is decomposed in the enzymatic of direct use, and enzyme does not have life, so active change can not be caused when using the fresh fiber crops of ramie and cause ramee quality unstable, be directly used in irrigated farmland containing a large amount of phosphorus, nitrogen, potassium etc. in waste water after simultaneously coming unstuck, reduce cost.Present invention also offers a kind of preparation method of described enzyme preparation, simple to operate, facilitate large-scale industrial production.Present invention also offers a kind of method using described enzyme preparation to come unstuck to ramie, simple to operate, the chemical reagent dosage used in autoclaving is little, reduces cost, simultaneously because employ enzyme preparation to avoid secondary pollution in scouring processes.
In order to set forth technical scheme of the present invention further, being below specific embodiments of the invention, and it should be noted that following examples are only most preferred embodiment of the present invention.
Embodiment 1
The preparation of enzyme preparation:
The chelating agent of ethoxylated dodecyl alcohol 5mL and 5mL being added 1000mL temperature is in 40 DEG C of water, stir, the first mixed liquor is obtained after ethoxylated dodecyl alcohol and chelating agent all dissolve, add in the first mixed liquor by alkaline pectase, neutral xylanase, dextranase, mannase, regulatory enzyme activity is: alkaline pectase 300U/ml; Neutral xylanase: 1000U/ml; Dextranase: 8000U/ml; Mannase: 1000U/ml.Obtain fresh retting enzyme preparation.
Embodiment 2
The ramie place of production: Hunan Yuanjiang shells numb efficiency; 265 kilograms of fresh fiber crops every day (being equivalent to 100 kilograms of dry fiber crops)
After using the fresh fiber crops of mechanical harvesting, green grass or young crops is gone in mechanical barking, obtains the fresh fiber crops of ramie, is immersed in the container filling enzyme preparation prepared by embodiment 1 by fresh for the ramie prepared fiber crops, carries out the operation of leaching enzyme, and leaching enzyme temperature is 35 DEG C; The leaching enzyme time is 28h; PH value is 8.5; Enzyme dosage accounts for the fresh numb total weight percent of ramie and is preferably 2.5%.Fresh for ramie fiber crops are taken out be placed on flax stamping device after the operation of leaching enzyme terminates and copy fiber crops operation in advance.Copy fiber crops 2 circle in advance.
Copy in advance fiber crops operation terminate after, will copy in advance the ramie after fiber crops fresh fiber crops dress cage, the every cage of 1200kg, then be placed on boil drift pond in, carry out autoclaving operation.Add autoclaving treatment fluid to boiling in drift pond, this treatment fluid comprises: NaSiO
336kg; 15kgNaOH; Poly (sodium aspartate) 12kg; H
2o
218kg, obtains mixture.Then heat this mixture at normal atmospheric pressure, temperature is 95 DEG C, and heat time heating time is preferably 1h, subsequently pressure is increased to 0.2MPa and continues the described mixed liquor 2h of heating.
After autoclaving process, fresher for the ramie after boiling fiber crops are placed on flax stamping device copy fiber crops 5 circle, thoroughly by as-spun fibre and pectin and lignin separation.Obtain as-spun fibre, then by as-spun fibre through overpickling 2 times, wash 2 times, oil, dry and obtain ramee.
The performance of the ramee that testing result and traditional chemical Degumming method obtain compares result as shown in Table 1 to Table 3:
The ramee Performance comparision that table 1, embodiment 2 obtain with dry numb chemical Degumming method
Table 2, embodiment 2 and the recombing bar quality comparation doing numb chemical Degumming
| Project | Embodiment 2 | Chemical Degumming |
| Runner rate % | 0.26 | 0.32 |
| Fiber crops grain grain/g | 5 | 7 |
| Average fiber length cm | 9.5 | 9.13 |
| Short fiber rate % | 4.95 | 5.4 |
Table 3, embodiment 2 and dry numb chemical Degumming 36Nm ramie yarn quality comparation
| Project | Unit | FZ/T3200-2003 is excellent | Embodiment | Chemical Degumming |
| Actual count | Nm | - | 36.49 | 36.23 |
| Weight unevenness | ％ | ≤3 | 1.66 | 2.6 |
| Deviation of weight | ％ | ±2.5 | -1.36 | -0.64 |
| The index of quality | - | - | 1775 | 1659 |
| The black board sliver uniformity | Point | 100 | 100 | 90 |
| Large joint | Individual/800m | 0 | 0 | 0 |
| Trifle | Individual/800m | 10 | 6 | 8 |
| Fiber crops grain | Individual/800m | 20 | 17 | 19 |
| Strip dry uniformity variant coefficient | ％ | ≤21 | 18.27 | 18.91 |
| Single thread fracture strength | CN/tex | ≥19 | 24.08 | 22.46 |
| The single thread force coefficient of variation | ％ | ≤19 | 17.09 | 19.52 |
| Extension at break | ％ | - | 5.12 | 2.89 |
Embodiment 3
The ramie place of production: Hunan Yuanjiang
After using the fresh fiber crops of mechanical harvesting, green grass or young crops is gone in hand peeling, obtains the fresh fiber crops of ramie, is immersed in the container filling enzyme preparation prepared by embodiment 1 by fresh for the ramie prepared fiber crops, carries out the operation of leaching enzyme, and leaching enzyme temperature is 35 DEG C; The leaching enzyme time is 30h; PH value is 8.5; Enzyme dosage accounts for the fresh numb total weight percent of ramie and is preferably 2.5%.Fresh for ramie fiber crops are taken out be placed on flax stamping device after the operation of leaching enzyme terminates and copy fiber crops operation in advance.Copy fiber crops 3 circle in advance.
Copy in advance fiber crops operation terminate after, will copy in advance the ramie after fiber crops fresh fiber crops dress cage, the every cage of 1200kg, then be placed on boil drift pond in, carry out autoclaving operation.Add autoclaving treatment fluid to boiling in drift pond, this treatment fluid comprises: NaSiO
336kg; 20kgNaOH; Poly (sodium aspartate) 12kg; H
2o
218kg, obtains mixture.Then heat this mixture at normal atmospheric pressure, temperature is 95 DEG C, and heat time heating time is 1h, subsequently pressure is increased to 0.2MPa temperature and continues the described mixed liquor 2h of heating.
After autoclaving process, fresher for the ramie after boiling fiber crops are placed on flax stamping device copy fiber crops 5 circle, thoroughly by as-spun fibre and pectin and lignin separation.Obtain as-spun fibre, then by as-spun fibre through overpickling 2 times, wash 2 times, oil, dry and obtain ramee.
It is as shown in table 4 that the performance of the ramee that testing result and traditional chemical Degumming method obtain compares result:
Table 4, embodiment 3 and dry numb biological bacteria obtain the Performance comparision of ramee after coming unstuck
Embodiment 4
In the waste water using potassium dichromate standard method mensuration embodiment 2 embodiment 3 and dry numb chemical Degumming to produce, COD's compares
The full glass refluxing unit of instrument: 250mL, heater (electric furnace), 50mL acid buret, conical flask, pipette, volumetric flask etc.
Reagent: potassium bichromate standard liquid (c
1/ 6K
2cr
2o
7=0.2500mol/L), ferroin indicator solution, iron ammonium sulfate standard liquid [c (NH
4)
2fe (SO
4)
26H
2o=0.1mol/L] (demarcating before using), sulfuric acid-silver sulfate solution.
Determination step:
Iron ammonium sulfate is demarcated: accurately draw 10.00mL potassium bichromate standard liquid in 500mL conical flask, be diluted with water to about 110mL, slowly add the 30mL concentrated sulfuric acid, shake up. after cooling, add 3 ferroin indicator solutions (0.15mL), use l ferrous ammonium sulfate solution titration, the color from yellow of solution is terminal through aquamarine blue to bronzing.
Measure:
Get 20mL water sample, add the potassium bichromate of 10mL, plug reflux, then add 30mL sulfuric acid-silver sulfate, add hot reflux 2h;
After cooling, rinse condensation tube wall with 90.00mL water, fetch the solution after stream;
After solution cools once again, add 3 ferroin indicator solutions, with the titration of iron ammonium sulfate standard liquid, the color from yellow of solution is terminal through aquamarine blue to bronzing, the consumption of record iron ammonium sulfate standard liquid;
While measuring water sample, get 20.00mL double distilled water, make blank assay by same operating procedure. the consumption of iron ammonium sulfate standard liquid when record titration is blank.
Calculate:
CODCr(O
2，mg/L)＝[8×1000(V
0-V
1)×C]/V；
C---the concentration of iron ammonium sulfate standard liquid, mol/L
V---volume of water sample, mL
V
0---the consumption of iron ammonium sulfate standard liquid when titration is blank, mL
V
1---the consumption of iron ammonium sulfate standard liquid during Titration Water Sample, mL
In formula, (V
0-V
1) × C is the amount of water pollutant (reducing substances), and the amount of oxidizing substance can trim.When being converted into oxygen demand, because lose an electronics in an iron ammonium sulfate molecular reaction, calculate by oxygen, an oxygen atom needs 2 electronics, and the atomic weight of oxygen atom is 16, so the quality obtaining a mole electron is 8 grams.Want calculated mass, so the amount of handy reducing substances is multiplied by 8 grams/mol of oxygen, income value is the oxygen demand of pollutant.
Result of calculation is as shown in table 5:
The detection of pH value uses accurate pH test paper testing result as shown in table 5:
Table 5, embodiment 2 embodiment 3 contrast with the discharge of wastewater index of dry numb chemical Degumming
| Embodiment 2 | Embodiment 3 | Conventional chemical comes unstuck | |
| COD | 9700 | 9850 | 18700 |
| pH | 12 | 12 | 13 |
The COD of boiling-off waste liquid comes unstuck will reduce 50% than conventional chemical.
Above the method for a kind of China grass degumming enzyme preparation provided by the invention and preparation method thereof and China grass degumming is described in detail, apply specific case herein to set forth principle of the present invention and embodiment, the explanation of above embodiment just understands method of the present invention and core concept thereof for helping, should be understood that, for those skilled in the art, under the premise without departing from the principles of the invention, some improvement and modification can also be carried out to the present invention, these improve and modify and also fall in the protection domain of the claims in the present invention.
Claims (5)
1. a method for the fresh retting of ramie, is characterized in that, comprising:
Green grass or young crops is gone to obtain ramie fresh fiber crops the ramie peeling of having gathered in;
Fresh for described ramie fiber crops are soaked 20h ~ 30h in enzyme preparation, and described enzyme preparation is that the alkaline pectase of 50U/mL ~ 5000U/mL and hemicellulase form by enzyme activity;
Described hemicellulase is made up of following biology enzyme: the dextranase that the neutral xylanase that enzyme activity is 500U/mL ~ 2000U/mL, enzyme activity are 4000U/mL ~ 50000U/mL, enzyme activity are the mannase of 600U/mL ~ 50000U/mL;
Also comprise with enzyme preparation by volume percentage be the surfactant of 0.1% ~ 10% and the chelating agent of 0.1% ~ 10%;
Described surfactant is ethoxylated dodecyl alcohol, and the oleophylic hydrophilic group equilibrium index of ethoxylated dodecyl alcohol is 10 ~ 16;
Described chelating agent is acrylic acid, two or more atactic polymer formed in acrylate, maleic anhydride, and the degree of polymerization of described chelating agent is 100 ~ 300;
It is 1.5% ~ 2.5% that the consumption of described enzyme preparation accounts for the fresh numb total weight percent of ramie;
The fresh fiber crops of ramie after described immersion enzyme preparation are copied fiber crops in advance on flax stamping device;
Copy the fresh fiber crops of the ramie after fiber crops in advance carry out autoclaving by described;
Copy the fresh fiber crops of the ramie after fiber crops in advance put into water by described, then add NaSiO in water
3, NaOH, auxiliary agent, H
2o
2obtain mixture;
Pressure is standard atmospheric pressure, temperature is heat described mixture 0.5h ~ 1h at 90 DEG C ~ 100 DEG C, then described pressure is increased to 0.2MPa, continues the described mixed liquor 0.5h ~ 2.5h of heating;
Fresh for ramie after described autoclaving fiber crops are copied fiber crops on flax stamping device, obtains as-spun fibre;
Described as-spun fibre is carried out pickling, washing, and oiling to dry after washing away impurity obtains ramee.
2. Degumming method according to claim 1, is characterized in that, described auxiliary agent is poly (sodium aspartate).
3. the fresh retting enzyme preparation of ramie that uses of claim 1, it is characterized in that, be that the alkaline pectase of 50U/mL ~ 5000U/mL and hemicellulase form by enzyme activity;
Described hemicellulase is made up of following biology enzyme: the dextranase that the neutral xylanase that enzyme activity is 500U/mL ~ 2000U/mL, enzyme activity are 4000U/mL ~ 50000U/mL, enzyme activity are the mannase of 600U/mL ~ 50000U/mL;
Also comprise with enzyme preparation by volume percentage be the surfactant of 0.1% ~ 10% and the chelating agent of 0.1% ~ 10%;
Described surfactant is ethoxylated dodecyl alcohol, and the oleophylic hydrophilic group equilibrium index of ethoxylated dodecyl alcohol is 10 ~ 16;
Described chelating agent is acrylic acid, two or more atactic polymer formed in acrylate, maleic anhydride, and the degree of polymerization of described chelating agent is 100 ~ 300.
4. a preparation method for the fresh retting enzyme preparation of ramie described in claim 3, is characterized in that, comprising:
Surfactant and chelating agent being added temperature is in the water of 30 DEG C ~ 50 DEG C, stirs, and dissolves, obtains the first mixed liquor after cooling;
Alkaline pectase, neutral xylanase, dextranase, mannase are added in the first mixed liquor respectively, obtains enzyme preparation.
5. preparation method according to claim 4, it is characterized in that, in described enzyme preparation, the enzyme activity of various enzyme is: alkaline pectase 50U/mL ~ 5000U/mL, neutral xylanase 500U/mL ~ 2000U/mL, dextranase 4000U/mL ~ 50000U/mL, mannase 600U/mL ~ 50000U/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010119192.XA CN102191566B (en) | 2010-03-04 | 2010-03-04 | The method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010119192.XA CN102191566B (en) | 2010-03-04 | 2010-03-04 | The method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102191566A CN102191566A (en) | 2011-09-21 |
| CN102191566B true CN102191566B (en) | 2015-11-25 |
Family
ID=44600403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010119192.XA Active CN102191566B (en) | 2010-03-04 | 2010-03-04 | The method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102191566B (en) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206874B (en) * | 2010-03-30 | 2014-07-30 | 上海康地恩生物科技有限公司 | Degumming method of flax and enzyme preparation for using in degumming |
| CN102747434A (en) * | 2012-04-06 | 2012-10-24 | 安徽省华龙麻业有限公司 | Compound biological enzyme degumming method for ramie |
| CN102704269B (en) * | 2012-05-21 | 2015-06-03 | 江南大学 | Method for improving composite performance of laccase induced plant fiber grafted acrylic ester with resin |
| CN103132153B (en) * | 2013-03-18 | 2015-04-01 | 河南舒莱卫生用品有限公司 | Preparation method and application of antibacterial ramie fiber |
| CN103114338B (en) * | 2013-03-18 | 2014-08-20 | 河南舒莱卫生用品有限公司 | Preparation method of ramie stalk antibacterial viscose fiber and application thereof |
| CN104726938B (en) * | 2013-12-19 | 2017-07-28 | 江苏悦达纺织集团有限公司 | A kind of cattail fiber and preparation method thereof |
| CN103774245B (en) * | 2014-02-25 | 2016-04-27 | 武汉纺织大学 | Ramie chain type continuous is without the useless point method for fiber that comes unstuck |
| CN104947199A (en) * | 2014-03-26 | 2015-09-30 | 中国科学院天津工业生物技术研究所 | Ramie degumming method |
| CN104264232B (en) * | 2014-08-27 | 2016-04-06 | 青岛大学 | The method of functional bast fiber is prepared in a kind of modification of coming unstuck |
| CN105734684A (en) * | 2016-04-01 | 2016-07-06 | 河北国轩麻纤维开发有限公司 | Degumming agent special for ramie and preparation method and application of degumming agent |
| CN106012042B (en) * | 2016-06-24 | 2018-08-14 | 中国农业科学院麻类研究所 | Mulch ramie degumming method |
| CN107385523A (en) * | 2017-07-19 | 2017-11-24 | 苏州凯邦生物技术有限公司 | A kind of biological enzyme jute fiber degumming agent |
| CN107326532A (en) * | 2017-07-24 | 2017-11-07 | 厦门盛方生态技术有限公司 | A kind of production method of ramee mattress |
| CN108118416B (en) * | 2018-02-07 | 2019-09-13 | 湖南麻布仕服饰股份有限公司 | A kind of bundle fiber fiber crops and the blended fabric and its preparation process of air-conditioning fiber |
| CN108708173A (en) * | 2018-06-11 | 2018-10-26 | 安徽省创安体育用品有限公司 | A kind of processing method improving apocynum fibre spinnability |
| CN109137089A (en) * | 2018-09-07 | 2019-01-04 | 嘉兴卓盛生物科技有限公司 | A kind of ramie soak and combination of enzyme preparations object and its application method for ramie raw materials degumming |
| CN112080483A (en) * | 2019-06-13 | 2020-12-15 | 青岛奥洛思新材料有限公司 | Hemp fiber degumming composite enzyme and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
| CN1624213A (en) * | 2004-12-10 | 2005-06-08 | 湖南农业大学 | Physical degumming method of ramie |
| CN101451132A (en) * | 2007-11-28 | 2009-06-10 | 青岛康地恩生物科技有限公司 | Complex enzyme preparation for China grass degumming as well as preparation method and use thereof |
| CN101649494A (en) * | 2009-09-04 | 2010-02-17 | 吴鹏 | Novel biological degumming agent for ramie raw materials |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI20065121L (en) * | 2006-02-17 | 2007-08-18 | Valtion Teknillinen | Method for pretreatment of cellulose-based textile materials |
-
2010
- 2010-03-04 CN CN201010119192.XA patent/CN102191566B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
| CN1624213A (en) * | 2004-12-10 | 2005-06-08 | 湖南农业大学 | Physical degumming method of ramie |
| CN101451132A (en) * | 2007-11-28 | 2009-06-10 | 青岛康地恩生物科技有限公司 | Complex enzyme preparation for China grass degumming as well as preparation method and use thereof |
| CN101649494A (en) * | 2009-09-04 | 2010-02-17 | 吴鹏 | Novel biological degumming agent for ramie raw materials |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102191566A (en) | 2011-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102191566B (en) | The method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie | |
| Csiszár et al. | Enzymes and chelating agent in cotton pretreatment | |
| Calafell et al. | Effect of some process parameters in the enzymatic scouring of cotton using an acid pectinase | |
| Vankar et al. | Ecofriendly ultrasonic natural dyeing of cotton fabric with enzyme pretreatments | |
| Battan et al. | Application of thermostable xylanase of Bacillus pumilus in textile processing | |
| CN101914848B (en) | Pre-treatment auxiliary agent for cotton knitted fabrics, preparation method and application thereof | |
| CN101463502B (en) | Pineapple leaf fiber biochemical degumming technique | |
| CN100999869A (en) | Textile printing and dyeing pretreating agent and application thereof | |
| CN103555702B (en) | The production method of chemical-mechanical pulping complex enzyme liquid and application | |
| Ossola et al. | Scouring of flax rove with the aid of enzymes | |
| CN104342425B (en) | For improving fiber oxidation enzymatic compositions and papermaking process and the application of Paper White Degree | |
| CN101643998A (en) | Treatment method of textile | |
| CN107345089A (en) | A kind of glass deinking agent | |
| CN105586799A (en) | Method for extracting nano-crystalline cellulose microfiber from cotton stalk bark | |
| CN107385523A (en) | A kind of biological enzyme jute fiber degumming agent | |
| CN111205934A (en) | Cleaning agent suitable for descaling of landfill leachate evaporator, and preparation method and application thereof | |
| WO2022127940A1 (en) | Biological enzymatic dyeing method for improving strength of wool fiber | |
| CN106120295A (en) | A kind of glycerol sodium carboxymethyl cellulose Compositional type hydrogen peroxide bleaching stabilizer and application thereof | |
| Zheng et al. | A sustainable one-step pretreatment of cotton gray fabric with 18-crown-6 as phase transfer in supercritical carbon dioxide | |
| Csiszár et al. | Degradation of lignin-containing materials by xylanase in biopreparation of cotton | |
| Ren et al. | A green process for flax fiber extraction by white rot fungus (Laccase mediators system) in a less-water environment | |
| Singh et al. | Environmental pollution reducing strategy for scouring of undegummed sisal fibers using xylanase and pectinase enzymes | |
| CN109989114A (en) | A kind of method of flax degumming | |
| Wu et al. | Identification, characteristics and rice growth promotion of a highly efficient cellulolytic bacterial strain, Cellulomonas iranensis ZJW-6, isolated from paddy soil in central China | |
| CN106433659A (en) | Repairing additive for treating soil heavy metal pollution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20151015 Address after: 201203 C307 room, No. 456 blue wave road, Zhangjiang hi tech park, Shanghai, Pudong New Area Applicant after: Shanghai KDN Biotech Co. Ltd. Applicant after: Qingdao Weilan Biology Group Co., Ltd. Address before: 201203 C307 room, No. 456 blue wave road, Zhangjiang hi tech park, Shanghai, Pudong New Area Applicant before: Shanghai KDN Biotech Co. Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |