CN102191162A - Real time detecting apparatus and method for microbial activity in air - Google Patents
Real time detecting apparatus and method for microbial activity in air Download PDFInfo
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- CN102191162A CN102191162A CN2010101279343A CN201010127934A CN102191162A CN 102191162 A CN102191162 A CN 102191162A CN 2010101279343 A CN2010101279343 A CN 2010101279343A CN 201010127934 A CN201010127934 A CN 201010127934A CN 102191162 A CN102191162 A CN 102191162A
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Abstract
The invention provides a real time detecting apparatus and a method for microbial activity in air. The real time detecting apparatus comprises a sampling pump, a fixing base, a fixing cannula, a detecting tube and a luminescence apparatus. The method comprises that: a desiring place is divided into a plurality of detection areas; with the sampling pump, indoor air is introduced into the fixing cannula through an air inlet pipe provided on the fixing cannula in each detection area, such that microbes in air adhere to a sterilized cotton stick of the detecting tube provided in the fixing cannula; after sampling, a transparent cannula of the detecting tube is sleeved on the sterilized cotton stick; a sealed column in one side of the detecting tube is broken, such that the sterilized cotton stick is immersed in a luciferase agent; the detecting tube is placed into a detecting aperture of the luminescence apparatus to analyze relative luminous quantity of the detecting tube through the luminescence apparatus; detected values of each area in the detected place are input into a computer; the detected values are collected through the computer to complete an isogram of the detected results to quickly obtain various amounts of the microbes in air in each area in the detected place area.
Description
Technical field
The present invention relates to microorganism active detecting real-time device and method in a kind of air, be particularly related to and a kind ofly in the place of desire detecting, be divided into a plurality of zones, gather the sample of microorganism in the air with detector tube, again each sample is placed cold light instrument, luminous quantity by cold light instrument real-time analysis sample, import again and try to achieve equal-value map in the computer, can learn the method for microbial biomass number in this each regional air of detecting place fast.
Background technology
Have many microorganisms (as bacterium) in the air, for detecting contained amount of bacteria in the air of indoor place.Present air detection mode mainly uses impact-actuated sampler 1 (as shown in Figure 1) to carry out the collection of sample.Detecting person must carry out the investigation and the field observation in indoor place earlier before the sampling, judges the worst position of this IAQ (indoor air quality), to obtain best detecting result.This impact-actuated sampler 1 is placed on this detecting position, and the flow process of this method for detecting is taken out the air sample that draws this indoor an amount of volume by impact-actuated sampler 1 as shown in Figure 2, takes out to draw the time and generally be set at two to eight minutes.Must sample several times in same fixed point, its sampling time can be respectively two minutes, three minutes, five minutes or eight minutes etc., and each is gathered once.Air is entered by the inlet mouth 12 of the lid 11 on the bearing 10 of impact-actuated sampler 1, in bearing 10, inserted culture dish 13, insert the substratum 14 of suitable microorganism growth in this culture dish 13, the air that is entered by inlet mouth 12 is directly impacted on culture dish 13, microorganism then is attached on the substratum 14, to gather again sample place cultivate growth in 48 hours ± 2 hours under 30 ± 1 ℃ the temperature after, count the colony number of microorganism on the substratum 14 again in the range estimation mode, finish the detecting of microorganism in the air, so that the improvement foundation of this detecting place Air quality to be provided.
Yet, existing detecting mode, the detecting personnel must possess the training that specialty is arranged, and need reach detection time more than 48 hours, and are very time-consuming, bothersome, and the expense of detecting is very expensive; This again detecting mode is because of the expense costliness, thus in a space, mostly only detect a point as reference, and the growth of different microorganisms bacterial classification differs, and the testing staff must possess the ability of difference bacterium and fungi, so counting is comparatively difficult.Existing method for detecting is to adopt quantitative detecting mode, and its detected result can't obtain best accuracy.
Summary of the invention
Main purpose of the present invention is to provide microorganism active detecting real-time device and method in a kind of air, be in the place of desire detecting, to be divided into several zones to carry out the detecting of microorganism active, can learn the microbial biomass number in each regional air after the detecting at once, can make things convenient for and finish detecting fast, and can significantly reduce the detecting expense.
Device of the present invention is provided with: a sampling pump is equipped with the conduit of bleeding on this sampling pump; One permanent seat is located at a side of sampling pump, and this permanent seat is provided with perforation; One fixed sleeving is arranged in the perforation of permanent seat, and this fixed sleeving is made as hollow form, end in fixed sleeving is provided with a flange, this flange is provided with patchhole, is provided with an inlet pipe and an escape pipe in the other end of fixed sleeving, and the conduit of bleeding of sampling pump is arranged on the escape pipe; One detector tube is inserted in the fixed sleeving, is provided with the envelope post in the end of this detector tube, is injected with fluorescence enzyme agent (luciferase) in this end, is provided with aseptic cotton rod in the other end of detector tube, the outer transparent casing that is arranged with of this aseptic cotton rod; One cold light instrument is for the luminous quantity on the aseptic cotton rod that detects detector tube.
Method for detecting of the present invention, its step is as follows:
(a) sampling pump is bled: set sampling pump and bleed the time, by starting the sampling pump running, the air in detecting place is taken out in the introducing fixed sleeving by the inlet pipe of fixed sleeving;
(b) microorganic adhesion is on the aseptic cotton rod of detector tube: then be attached on the aseptic cotton rod of detector tube by microorganism in the air of the introducing that inlet pipe is taken out of fixed sleeving;
(c) detector tube is inserted in the cold light instrument: the detector tube after will sampling takes off on fixed sleeving, and transparent casing is sheathed on the aseptic cotton rod, the envelope post of detector tube one end is fractureed, make the interior fluorescence enzyme agent of detector tube by flowing in the transparent casing in the aseptic cotton rod, aseptic cotton rod be impregnated in the fluorescence enzyme agent, again detector tube is placed in the mensuration hole of cold light instrument, covers loam cake again;
(d) cold light instrument is analyzed the relative luminous quantity of detector tube: utilize cold light instrument adenosine triphosphate biologic ferment reaction inspection method, analyze the microorganism on the aseptic cotton rod after the sampling and fluorescence enzyme agent in the detector tube through cold light luminous quantity that oxygenolysis produced, bacterium number on the aseptic cotton rod is many more, its cold light signal is strong more, can be obtained the measured value of microbial luminescence amount fast by cold light instrument;
(e) the mensuration numerical value that will detect each zone, place is imported computer: will detect the place and be divided into several zones, and repeatedly sample respectively in each zone, respectively with obtained respectively the measuring in the numerical value input computer of each sampling;
(f) finish equal-value map: compile the obtained detecting value in each territory of detecting the place by computer, can finish detecting result's equal-value map.
Description of drawings
Figure 1 shows that the cross-sectional schematic of existing impact-actuated sampler;
Figure 2 shows that the schema of microorganism method for detecting in the existing air;
Figure 3 shows that the three-dimensional exploded view of embodiment of the invention arrangement for detecting;
Figure 4 shows that the cross-sectional schematic of embodiment of the invention arrangement for detecting;
Figure 5 shows that the synoptic diagram of embodiment of the invention detector tube and cold light instrument;
Figure 6 shows that the schema of embodiment of the invention method for detecting;
Figure 7 shows that the traditional Chinese medical science of the embodiment of the invention in hospital examine between the detecting after equal-value map;
Figure 8 shows that the equal-value map of the embodiment of the invention after the detecting of the hall of hospital;
Figure 9 shows that the equal-value map of the embodiment of the invention after hospital's Stall detecting;
Figure 10 shows that the equal-value map of the embodiment of the invention after managing the detecting of hall, station;
Figure 11 shows that the equal-value map of the embodiment of the invention after the detecting of large-scale mass merchant;
Figure 12 shows that the equal-value map of the embodiment of the invention after Library's detecting.
Description of reference numerals: 1-impact-actuated sampler; The 10-bearing; The 11-lid; The 12-inlet mouth; The 13-culture dish; The 14-substratum; The 2-sampling pump; The 20-conduit of bleeding; The 3-permanent seat; The 30-perforation; The 4-fixed sleeving; The 40-flange; The 41-patchhole; The 42-inlet pipe; The 43-escape pipe; The 5-detector tube; 50-seals post; 51-fluorescence enzyme agent; The aseptic cotton rod of 52-; The 53-transparent casing; The 6-cold light instrument; 60-measures the hole; The 61-loam cake.
Embodiment
Be described in more detail with other technical characterictic and advantage the present invention is above-mentioned below in conjunction with drawings and Examples, it should be understood that these embodiment only are used for the purpose of illustration, never limit protection scope of the present invention.
Embodiments of the invention see also Fig. 3～shown in Figure 5, and it mainly is provided with a sampling pump 2, are equipped with the conduit 20 of bleeding on this sampling pump 2; One permanent seat 3 is located at a side of sampling pump 2, and this permanent seat 3 is provided with a perforation 30; One fixed sleeving 4 is arranged in the perforation 30 of permanent seat 3, and this fixed sleeving 4 is made as hollow form, is provided with a flange 40 in an end of fixed sleeving 4, and this flange 40 is provided with patchhole 41, is provided with an inlet pipe 42 and an escape pipe 43 in the other end of fixed sleeving 4; One detector tube 5 is inserted in the fixed sleeving 4, is provided with envelope post 50 in the end of this detector tube 5, is injected with fluorescence enzyme agent 51 in this end, is provided with aseptic cotton rod 52 in the other end of detector tube 5, these aseptic cotton rod 52 outer transparent casings 53 that are arranged with; One cold light instrument 6, the luminous quantity on the aseptic cotton rod 52 that detects detector tube 5 is provided with one and measures hole 60 in this cold light instrument 6, and cold light instrument 6 is provided with a loam cake 61.
Wherein said detector tube 5 is provided with envelope post 50 in the one end, the liquid that injects in this end can comprise fluorescein (luciferin) or fluorescence ferment (luciferase), the about 2.5ml of this liquid; The other end of detector tube 5 is provided with aseptic cotton rod 52, the liquid that can contain the 0.2ml that has an appointment at this cotton end, this liquid comprises damping fluid (buffer components) and cleaning mixture (detergent component), the main effect of the two is the cytolemma of puncture destroy microorganisms, makes it be built-up in sampling cotton end.
Method for detecting, see also Fig. 3～shown in Figure 6, by fixed sleeving 4 being arranged in the perforation 30 of permanent seat 3, fixed sleeving 4 is fixed on the permanent seat 3, the conduit 20 of bleeding with sampling pump 2 is arranged on the escape pipe 43 of fixed sleeving 4 again, transparent casing 53 with detector tube 5 takes off in addition, the aseptic cotton rod 52 of detector tube 5 is inserted in the patchhole 41 of fixed sleeving 4, detector tube 5 is sheathed on the flange 40 of fixed sleeving 4, and make aseptic cotton rod 52 between the inlet pipe 42 of fixed sleeving 4 and escape pipe 43 (as shown in Figure 4), so can detect.
It detects step following (as shown in Figure 6):
(a) sampling pump is bled: that sets sampling pump 2 bleeds the time that (the best time of bleeding is two to eight minutes, same detecting point can be sampled several times to obtain the optimum detection value, its sampling time can be respectively two minutes, three minutes, five minutes or eight minutes etc., and each is gathered once), by starting sampling pump 2 runnings, the air in detecting place is taken out in the introducing fixed sleeving 4 by the inlet pipe 42 of fixed sleeving 4;
(b) microorganic adhesion is on the aseptic cotton rod of detector tube: then be attached to by microorganism in the air of inlet pipe 42 introducing of taking out of fixed sleeving 4 on the aseptic cotton rod 52 of detector tube 5;
(c) detector tube is inserted in the cold light instrument: the detector tube 5 after will sampling takes off on fixed sleeving 42, and transparent casing 53 is sheathed on the aseptic cotton rod 52, envelope post 50 with detector tube 5 one ends is fractureed again, fluorescence enzyme agent 51 in the detector tube 5 is flowed in the transparent casings 53 in aseptic cotton excellent 52, aseptic cotton rod 52 be impregnated in the fluorescence enzyme agent 51, detector tube 5 is placed in the mensuration hole 60 of cold light instrument 6 again, covers loam cake 61 again;
(d) cold light instrument is analyzed the relative luminous quantity of detector tube: utilize cold light instrument 6 adenosine triphosphates (AdenosineTri Phosphate, be called for short ATP) biologic ferment reaction inspection method, analyze the microorganism on the aseptic cotton rod 52 after the sampling and fluorescence enzyme agent 51 in the detector tube 5 through cold light luminous quantity that oxygenolysis produced, bacterium number on the aseptic cotton rod 52 is many more, its cold light signal is strong more, can be obtained the measured value of microbial luminescence amount fast by cold light instrument 6;
(e) the mensuration numerical value that will detect each zone, place is imported computer: will detect the place and be divided into several zones, and repeatedly sample respectively in each zone, respectively with obtained respectively the measuring in the numerical value input computer of each sampling; And how to divide the detecting place the zone, because of interior space shape changeable, therefore as far as possible with the system mode planning space zone of triangular, eight points are detected in each space at least, so must divide eight zones at least, record unusual high value or space when big when the observation space situation has obvious pollution source, scene, can increase the detecting point in real time, to improve the microorganism active distribution characteristics;
(f) finish equal-value map: compile detecting each territory, place obtained detecting value by computer, can finish detecting result's equal-value map (as Fig. 7～shown in Figure 12).
The present invention is between the traditional Chinese medical science in hospital is examined on the spot respectively with above-mentioned method for detecting, the hall of hospital, hospital's Stall, manage the hall, station, the microorganism active detecting is carried out in places such as large-scale mass merchant and Library, detecting result's equal-value map as shown in Figure 7 between the traditional Chinese medical science in hospital was examined, in the hall of hospital detecting result's equal-value map as shown in Figure 8, in hospital Stall detecting result's equal-value map as shown in Figure 9, in the equal-value map of managing hall, station detecting result as shown in figure 10, in the detecting result's of large-scale mass merchant equal-value map as shown in figure 11, in Library detecting result's equal-value map as shown in figure 12, wherein, yellow block represents that bacteria containing amount is high more in each equal-value map, and blue block represents that bacteria containing amount is lower.
By method for detecting of the present invention, the place of desire detecting can be divided into a plurality of zones repeatedly detects respectively, the detecting result in each zone all can learn fast, need not to wait as long for, can try to achieve the equal-value map of whole detecting place air performance number, the present invention adopts qualitative detecting mode, the Air quality that can judge which zone in this detecting place fast is relatively poor, can provide the dealer to carry out the improvement of Air quality at this zone, its detecting time is quick, and can reduce the detecting expense.
The above only is preferred embodiment of the present invention, only is illustrative for the purpose of the present invention, and nonrestrictive.Those skilled in the art is understood, and can carry out many changes to it in the spirit and scope that claim of the present invention limited, revise, even equivalence, but all will fall within the scope of protection of the present invention.
Claims (3)
1. microorganism active detecting real-time device in the air is characterized in that being provided with:
One sampling pump is equipped with the conduit of bleeding on this sampling pump;
One permanent seat is located at a side of sampling pump, and this permanent seat is provided with perforation;
One fixed sleeving is arranged in the perforation of permanent seat, and this fixed sleeving is a hollow form, end in fixed sleeving is provided with a flange, this flange is provided with patchhole, is provided with an inlet pipe and an escape pipe in the other end of fixed sleeving, and the conduit of bleeding of sampling pump is arranged on the escape pipe;
One detector tube is inserted in the fixed sleeving, is provided with the envelope post in the end of this detector tube, is injected with the fluorescence enzyme agent in this end, is provided with aseptic cotton rod in the other end of detector tube, the outer transparent casing that is arranged with of this aseptic cotton rod;
One cold light instrument is for the luminous quantity on the aseptic cotton rod that detects detector tube.
2. microorganism active detecting real-time method in the air is characterized in that its step is as follows:
(a) sampling pump is bled: set sampling pump and bleed the time, by starting the sampling pump running, the air in detecting place is taken out in the introducing fixed sleeving by the inlet pipe of fixed sleeving;
(b) microorganic adhesion is on the aseptic cotton rod of detector tube: then be attached on the aseptic cotton rod of detector tube by microorganism in the air of the introducing that inlet pipe is taken out of fixed sleeving;
(c) detector tube is inserted in the cold light instrument: the detector tube after will sampling takes off on fixed sleeving, and transparent casing is sheathed on the aseptic cotton rod, the envelope post of detector tube one end is fractureed, make the interior fluorescence enzyme agent of detector tube by flowing in the transparent casing in the aseptic cotton rod, aseptic cotton rod be impregnated in the fluorescence enzyme agent, again detector tube is placed in the mensuration hole of cold light instrument, covers loam cake again;
(d) cold light instrument is analyzed the relative luminous quantity of detector tube: utilize cold light instrument adenosine triphosphate biologic ferment reaction inspection method, analyze the microorganism on the aseptic cotton rod after the sampling and fluorescence enzyme agent in the detector tube through cold light luminous quantity that oxygenolysis produced, bacterium number on the aseptic cotton rod is many more, its cold light signal is strong more, can be obtained the measured value of microbial luminescence amount fast by cold light instrument;
(e) the mensuration numerical value that will detect each zone, place is imported computer: will detect the place and be divided into several zones, and repeatedly sample respectively in each zone, respectively with obtained respectively the measuring in the numerical value input computer of each sampling;
(f) finish equal-value map: compile the obtained detecting value in each territory of detecting the place by computer, can finish detecting result's equal-value map.
3. microorganism active detecting real-time method is characterized in that in the air as claimed in claim 2, and the best time of bleeding of sampling is two to eight minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010127934 CN102191162B (en) | 2010-03-19 | 2010-03-19 | Real time detecting apparatus and method for microbial activity in air |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010127934 CN102191162B (en) | 2010-03-19 | 2010-03-19 | Real time detecting apparatus and method for microbial activity in air |
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| Publication Number | Publication Date |
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| CN102191162A true CN102191162A (en) | 2011-09-21 |
| CN102191162B CN102191162B (en) | 2013-08-21 |
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| CN 201010127934 Expired - Fee Related CN102191162B (en) | 2010-03-19 | 2010-03-19 | Real time detecting apparatus and method for microbial activity in air |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390948A (en) * | 2014-11-24 | 2015-03-04 | 中国食品发酵工业研究院 | Method for detecting bacterioplankton in air |
| CN109364283A (en) * | 2018-11-13 | 2019-02-22 | 薄玉冰 | Medical air sterilizing machine intelligence control system based on ATP detection technique |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6293634A (en) * | 1985-10-18 | 1987-04-30 | Matsushita Seiko Co Ltd | Microbe counter |
| CN1218553A (en) * | 1997-01-15 | 1999-06-02 | 新眼界诊断公司 | Method for detection of contaminants |
| CN1957089A (en) * | 2004-03-01 | 2007-05-02 | 麦卡米特私人有限公司 | Measuring contamination |
-
2010
- 2010-03-19 CN CN 201010127934 patent/CN102191162B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6293634A (en) * | 1985-10-18 | 1987-04-30 | Matsushita Seiko Co Ltd | Microbe counter |
| CN1218553A (en) * | 1997-01-15 | 1999-06-02 | 新眼界诊断公司 | Method for detection of contaminants |
| CN1957089A (en) * | 2004-03-01 | 2007-05-02 | 麦卡米特私人有限公司 | Measuring contamination |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390948A (en) * | 2014-11-24 | 2015-03-04 | 中国食品发酵工业研究院 | Method for detecting bacterioplankton in air |
| CN109364283A (en) * | 2018-11-13 | 2019-02-22 | 薄玉冰 | Medical air sterilizing machine intelligence control system based on ATP detection technique |
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| Publication number | Publication date |
|---|---|
| CN102191162B (en) | 2013-08-21 |
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