TWM456366U - Device for real-time detecting microorganism activity in air - Google Patents

Description

Instant detection device for microbial activity in the air

The present invention relates to an instant detection device for microbial activity in the air, in particular to a region that is divided into a plurality of regions in a place to be detected, to detect a sample of microorganisms collected in the air, and then to place each sample in a luminometer In the meantime, by analyzing the illuminance of the sample by the luminometer, and then inputting the equivalent map into the computer, the amount of microbial biomass in the air of each area of the detection site can be quickly learned.

According to the presence of many microorganisms (such as bacteria) in the air, in order to detect the amount of bacteria contained in the air in the indoor space, the current air detection method mainly uses the impact sampler 1 (as shown in the first figure). For sample collection, the sampler must first conduct an indoor location survey and on-site observation to determine the location of the indoor air quality to obtain the best detection result, and place the impact sampler 1 In the detection position, the flow of the detection method is as shown in the second figure. The impact sampler 1 is used to draw an appropriate volume of air sample in the room, and the extraction time is generally set to two to eight minutes. The same fixed point must be sampled multiple times, and the sampling time can be collected once every two minutes, three minutes, five minutes or eight minutes, and the air is taken from the air inlet of the cover 11 on the bearing 10 of the impact sampler 1. 12 enters, and a petri dish 13 is built in the socket 10, and the petri dish 13 is built into a suitable micro-generation The medium 14 for growing the material causes the air entering through the air inlet 12 to directly impinge on the culture dish 13, the microorganisms are attached to the medium 14, and the collected sample is cultured at a temperature of 30 ± 1 ° C for 48 hours ± 2 After the hour of growth, the number of microbes on the medium 14 is counted by visual inspection to complete the detection of microorganisms in the air to provide an improvement on the air quality of the detection site; however, the detection method of the detection method is required by the detection personnel. With professional training and detection time of up to 48 hours or more, it is extremely time-consuming and laborious, and the cost of detection is extremely expensive. This kind of detection method is expensive, so most of them are detected in one space. As a reference, and the growth of different microbial strains is different, the testers must have the ability to distinguish between bacteria and fungi, so the counting is difficult. The conventional detection method is based on quantitative detection, and the detection results cannot be optimal. The accuracy of this; therefore, the creator has deliberately studied and changed the method used to detect the total amount of microorganisms in the indoor air. , Then this is the first creation.

The main purpose of this creation is to provide an instant detection device for microbial activity in the air, which is divided into several areas for detection of microbial activity in the place to be detected, and the air in each area can be immediately known after detection. The amount of microbes can be easily and quickly detected, and the cost of detection can be greatly reduced.

The instant detection device for microbial activity in the air of the present invention is provided with a sampling pump, and the sampling pump is connected with a suction duct; a fixed seat is provided On one side of the sampling pump, the fixing seat is provided with a perforation; a fixing sleeve is disposed in the perforation of the fixing seat, the fixing sleeve is hollow, and a convex is arranged at one end of the fixing sleeve The flange is provided with an insertion hole, and an intake pipe and an air outlet pipe are disposed at the other end of the fixed sleeve, and the suction conduit of the sampling pump is connected to the air outlet pipe; The utility model is disposed in the fixed sleeve, wherein the detecting tube is provided with a sealing column in one end, the end portion is filled with a fluorescent enzyme agent, and the other end of the detecting tube is provided with a sterile cotton swab, and the sterile cotton swab outer sleeve is provided with a transparent Casing; a luminometer, which is used to detect the amount of luminescence on a sterile cotton swab.

For the purpose of achieving the above-mentioned purpose and effect of the above-mentioned use, the preferred embodiments are described, and the details are as follows: First, please refer to the third to fifth figures. The main assembly is provided with a sampling pump 2, and an extraction duct 20 is connected to the sampling pump 2; a fixing base 3 is disposed on one side of the sampling pump 2, and the fixing base 3 is provided with a through hole 30. a fixing sleeve 4 is disposed in the through hole 30 of the fixing base 3, the fixing sleeve 4 is hollow, and a flange 40 is disposed at one end of the fixing sleeve 4, and the flange 40 is provided on the flange 40 The insertion hole 41 is provided with an intake pipe 42 and an air outlet pipe 43 at the other end of the fixed sleeve 4; a detecting tube 5 is inserted into the fixed sleeve 4, and one end of the detecting tube 5 is disposed The sealing column 50 is filled with a fluorescent enzyme agent 51 at the end, and a sterile cotton swab 52 is disposed at the other end of the detecting tube 5, The sterilized cotton swab 52 is provided with a transparent sleeve 53; a luminometer 6 is provided for detecting the amount of illuminating light on the sterile cotton swab 52 of the detecting tube 5, and the luminometer 6 is provided with a measuring hole 60 on the luminometer 6 An upper cover 61 is provided.

The apparatus for detecting the microbial activity in the air is as follows. Please refer to the third to sixth figures. The fixing sleeve 4 is fixed on the fixing base 3, and the suction duct 20 of the sampling pump 2 is connected to the air outlet tube 43 of the fixed sleeve 4, and the transparent sleeve 53 of the detecting tube 5 is removed. The aseptic cotton swab 52 of the detecting tube 5 is inserted into the insertion hole 41 of the fixing sleeve 4, the detecting tube 5 is sleeved on the flange 40 of the fixing sleeve 4, and the sterile cotton swab 52 is located at the fixing sleeve 4. The air intake pipe 42 and the air outlet pipe 43 (as shown in the fourth figure) can be detected.

The detection steps are as follows (as shown in the sixth figure): (a) sampling pump for pumping: setting the sampling time of sampling pump 2 (the optimal pumping time is two to eight minutes, the same detection point can be Sampling multiple times to obtain the best detection value, the sampling time can be collected once every two minutes, three minutes, five minutes or eight minutes), by starting the sampling pump 2 operation, the air of the detection place will be The intake pipe 42 of the fixed sleeve 4 is drawn into the fixed sleeve 4; (b) the microorganism is attached to the sterile cotton swab of the detecting tube: the microorganisms in the air introduced by the intake pipe 42 of the fixed sleeve 4 are Attached to the sterile cotton swab 52 of the detecting tube 5; (c) the detecting tube is placed in the luminometer: the self-fixing sleeve of the detecting tube 5 after sampling 42 is removed, and the transparent sleeve 53 is sleeved on the sterile cotton swab 52, and the sealing column 50 at one end of the detecting tube 5 is broken, so that the fluorescent enzyme agent 51 in the detecting tube 5 is made of a sterile cotton swab. 52 flows into the transparent sleeve 53, so that the sterile cotton swab 52 is immersed in the fluorescent enzyme agent 51, and the detecting tube 5 is placed in the measuring hole 60 of the cold light meter 6, and then the upper cover 61 is closed; (d) The relative luminescence amount of the tube is analyzed by a luminometer. The sensitization method of the adenosine Tri Phosphate (ATP) bio-enzyme reaction detection method is used to analyze the aseptic cotton swab 52 after the sampling tube 5 is sampled. The amount of cold light emitted by the oxidative decomposition of the microorganism and the fluorescent enzyme agent 51, the more the number of bacteria on the sterile cotton swab 52, the stronger the cold light signal, and the rapid measurement of the amount of microbial luminescence by the luminometer 6 (e) input the measured values of each area of the detection site into the computer: divide the detection site into several areas, and perform multiple samplings in each area, and input the measured values obtained by each sampling into the computer; f) Completion of the equivalence map: the detection value obtained by each domain of the detection site is collected by the computer To complete the detection result of the contour maps (as shown in FIG seventh to twelve).

The above-mentioned detection methods are used to conduct microbial activity detection in the hospital's internal medicine clinic, hospital hall, hospital first floor, supervision station hall, large-scale retail stores and libraries, etc. The equivalent map of the detection results is shown in Figure 7. The equivalent map of the detection results in the lobby of the hospital is shown in Figure 8. The equivalent map of the results on the first floor of the hospital is as shown in the figure. As shown in Figure 9, the equivalent map of the results of the detection in the lobby of the supervision station is shown in the tenth figure. The equivalent map of the detection results in the large-scale retail stores is shown in Figure 11, and the results of the library detection are the same. The value map is shown in Fig. 12, wherein the yellow block in each isoform indicates that the higher the bacteria content, the blue block indicates that the bacteria content is lower. With the detection method of the present invention, the location to be detected can be divided into multiple regions for multiple detections, and the detection results of each region can be quickly learned, and the entire detection can be obtained without waiting for a long time. The equivalent of the air value of the test site, the creative system uses a qualitative detection method to quickly determine which area of the detection site has poor air quality, and can provide an improvement in the air quality of the area. Fast detection time and reduced detection costs.

In summary, the present invention has achieved the intended use and effect, and is more desirable and practical than the prior art. However, the above embodiments are only specifically described for the preferred embodiment of the present invention. This embodiment is not intended to limit the scope of the present invention, and all other equivalents and modifications may be included in the scope of the invention covered by the present invention.

1‧‧‧impact sampler

10‧‧‧ 承座

11‧‧‧ Cover

12‧‧‧air inlet

13‧‧‧ Petri dishes

14‧‧‧ medium

2‧‧‧Sampling pump

20‧‧‧Exhaust duct

3‧‧‧ Fixed seat

30‧‧‧Perforation

4‧‧‧Fixed casing

40‧‧‧Flange

41‧‧‧Into the hole

42‧‧‧Intake pipe

43‧‧‧Exhaust pipe

5‧‧‧Inspection tube

50‧‧‧Closed column

51‧‧‧Fluorescent enzyme

52‧‧‧Septic cotton swabs

53‧‧‧Transparent casing

6‧‧‧ luminometer

60‧‧‧Measurement hole

61‧‧‧Upper cover

The first figure shows a schematic cross-sectional view of a conventional impact sampler.

The second figure shows a flow chart of a conventional method for detecting microorganisms in the air.

The third figure is an exploded perspective view of the detecting device of the present embodiment.

The fourth figure is a schematic cross-sectional view of the detecting device of the present embodiment.

The fifth figure shows a schematic diagram of the detection tube and the luminometer of the present embodiment.

The sixth figure is a flow chart of the detection method of the present embodiment.

The seventh figure shows the equivalent map of the creative embodiment after detection in the hospital.

The eighth figure shows the equivalent map of the creative embodiment after detection in the lobby of the hospital.

The ninth figure is an equivalent diagram of the first embodiment of the present invention after being detected on the first floor of the hospital.

The tenth figure shows the equivalent diagram of the creation example in the lobby of the supervision station.

The eleventh figure shows the equivalent map of the creative embodiment after being detected by a large-scale retail store.

Figure 12 is a diagram showing the equivalent of the creative embodiment after the library is detected.

2‧‧‧Sampling pump

20‧‧‧Exhaust duct

3‧‧‧ Fixed seat

30‧‧‧Perforation

4‧‧‧Fixed casing

40‧‧‧Flange

41‧‧‧Into the hole

42‧‧‧Intake pipe

43‧‧‧Exhaust pipe

5‧‧‧Inspection tube

50‧‧‧Closed column

51‧‧‧Fluorescent enzyme

52‧‧‧Septic cotton swabs

Claims (1)

An instant detection device for microbial activity in the air is provided with a sampling pump, and an extraction duct is connected to the sampling pump; a fixing seat is disposed on one side of the sampling pump, and the fixing seat is provided with a perforation; a fixing sleeve is disposed in the through hole of the fixing seat, the fixing sleeve is hollow, and a flange is arranged at one end of the fixing sleeve, and the flange is provided with an insertion hole for fixing the sleeve The other end is provided with an intake pipe and an air outlet pipe, and the suction pipe of the sampling pump is connected to the air outlet pipe; a detecting pipe is inserted into the fixed casing, and one end of the detecting pipe is provided a sealing column, the end portion is filled with a fluorescent enzyme agent, and the other end of the detecting tube is provided with a sterile cotton swab, the sterile cotton swab outer sleeve is provided with a transparent sleeve; and a cold light meter is used for detecting the aseptic cotton of the detecting tube The amount of luminescence on the stick.