CN102188515A - Purpose of the plant Sibiraea angustata - Google Patents

Abstract

The invention discloses application of Sibiraea angustata in preparing antifatigue medicaments or health products and in preparing antihypoxic medicaments or health products, or rather, application of water extract of S. laecigata (L) Maxim. in preparing antihypoxic medicaments or health products. According to results of related experiments in the invention, aqueous extract of Sibiraea angustata can enhance exercise tolerance of an animal, increase content of animal hepatic/muscle glycogen, alleviate oxygen deficit in extracellular fluid of the animal, and raise utilization efficiency of oxygen for cells. The experiments in the invention indicate that Sibiraea angustata can enhance both exercise tolerance and antianoxia capacity of an animal.

Description

A kind of purposes of plant willow tea

Technical field

The present invention relates to the purposes of a kind of plant, the present invention relates to willow tea exactly, promptly plant Xianbei an ancient nationality in China spends the application in certain medicine of preparation or preparation health product.

Background technology

Willow tea is Tibetan's medicine commonly used among the people, spend branch and leaf and the infructescence (Fruit branch of band fruit) of S.laecigata (L) Maxim. for flower plant narrow leaf Xianbei an ancient nationality in China of Rosaceae Xianbei an ancient nationality in China spends Sibiraeaangustata (Rehd.) Hand.-Mazz. and Xianbei an ancient nationality in China, be mainly used in dyspepsia and intestinal stasis relieving, dispelling wind and heat pathogens; The Tibetan medicine document records, and cures mainly calentura and eqpidemic disease.In Tibetan's traditional food, have and the branch of willow tea is simmered ripe with ash, or its infructescence taken after mixing it with water with clearing away stomach-heat, building body after with the little stir-fry of little fire.Also have the open willow tea of document to have: anti-liver injury, transfer fat, blood pressure lowering, the antitumor action of antioxidation, raising immunity etc.Chinese invention patent 200610041923.7 also discloses willow tea in the medicine of preparation treatment constipation or the application in the health product.

Summary of the invention

The invention provides the purposes of a kind of unexposed mistake still of willow tea.

Purposes of the present invention is: Xianbei an ancient nationality in China spends the application in preparation antifatigue medicine, or the application of the water extract spent of Xianbei an ancient nationality in China in preparation antifatigue medicine, or Xianbei an ancient nationality in China spends the application in preparation antifatigue health product, or the application of the water extract spent of Xianbei an ancient nationality in China in preparation antifatigue health product, or Xianbei an ancient nationality in China spends the application in the medicine of preparation anti-hypoxia, or the application of the water extract spent of Xianbei an ancient nationality in China in the medicine of preparation anti-hypoxia, perhaps Xianbei an ancient nationality in China spends the application in the health product of preparation anti-hypoxia, the application of the water extract that Xianbei an ancient nationality in China spends in the health product of preparation anti-hypoxia.

Relevant experiment shows that willow tea the water extracted immersing paste can increase the exercise tolerance of mice, also can improve Mouse Liver/muscle glycogen content; Experiment shows that also the outer liquid anoxia of mouse cell can be alleviated and prolong to willow tea the water extracted immersing paste, makes cell utilize the efficient of oxygen to improve.These experiment prompting willow tea can improve the exercise tolerance of animal, also can improve the ability of animal anti-hypoxia.

The specific embodiment

The experiment of embodiment one willow tea water extract resisting fatigue

1. material: Kunming mouse, body weight 18-22g.Male, Lanzhou University zoopery center provides.Willow tea is adopted in cooperation suburbs, Tibetan Autonomous Prefecture of Gannan, Gansu Province when the grassland, state, local height above sea level 3200m, and identify through pharmaceutical college of Lanzhou University pharmacognostical study.

Willow tea water extract obtains extractum through the rotary evaporation drying; Rhodiola rosea capsules: commercially available (development of Himalaya drug research center, Chinese Qinghai)

Hepatic glycogen and muscle glycogen test kit (Nanjing is built up bio-engineering research and provided); Normal saline; Ultraviolet-uisible spectrophotometer.

2. grouping administration: 100 of mices are divided into 5 groups, blank group (with the volume normal saline), positive controls (Rhodiola rosea capsules), willow tea the water extracted immersing paste high dose (3.8g/kg.d), middle dosage (1.9g/kg.d), low dosage (0.95g/kg.d) at random.Each organizes the continuous gastric infusion of mice carries out every index after 15 days mensuration.

3. experiment:

(1) willow tea the water extracted immersing paste is to the influence of mouse movement endurance: the mice last is got 10 for every group after irritating stomach 30min, at the lead of mouse tail load with respect to body weight 5%, places the swimming case swimming with a load attached to the body of (30 ± 2) ℃.Swim to head with mice and to go deep in the water 10s end that do not refloat, record mice swimming time.Experimental result sees Table 1.

Table 1

By table one as seen, the exercise tolerance of mice body is influenced the aspect, the basic, normal, high dosage of willow tea is compared blank group and is increased significantly.In the dosage group for the raising of exercise tolerance a little more than positive controls.Illustrate that willow tea water body thing can will improve the exercise tolerance of mice body.

(2) willow tea the water extracted immersing paste is to the influence of Mouse Liver/muscle glycogen content: every group of mice got 10, and not swimming with a load attached to the body places the swimming case of (30 ± 2) ℃ 90min that swims.Test liver/muscle glycogen content after taking out every mice 30min, collect liver, the muscular tissue of every mice.Test liver/muscle glycogen successively according to liver/muscle glycogen testing cassete that Nanjing is built up bio-engineering research and provided.Experimental result sees Table 2

Table 2

By table two as seen, to the influence of mice biochemical indicator aspect, the basic, normal, high dosage of willow tea water extract is compared the blank group of liver/muscle glycogen content that can obviously improve body.The liver of basic, normal, high dosage group/muscle glycogen content is lower than positive controls, and its reason is likely because the positive controls of choosing is a compound preparation, is not a simple kind of plant.

As known from the above, high height above sea level living environment makes willow tea possess special adaptability material from heredity.The special chemical constituent that willow tea is contained makes it have some special biological function.Visible by experiment willow tea has certain positive role for the raising of macroscopic motion endurance; Willow tea can increase body hepatic glycogen, muscle glycogen deposit on biochemical indicator, improves body carbohydrate metabolism system energy supply level, reduces the ratio of body protein energy supply.

The research of embodiment two willow tea anti-hypoxia

1 test material

1.1 material and instrument

Willow tea is gathered with embodiment 1.

Rhodiola rosea capsules: commercially available (development of Himalaya drug research center, Chinese Qinghai)

1.2 laboratory animal

Kunming mouse, mice were 6～8 ages in week, body weight (25 ± 3) g, and Lanzhou University's medical college Experimental Animal Center provides.

Animal housing's temperature is controlled at 20 ℃～25 ℃, and relative humidity is 45%-55%,

Normal feedstuff: Lanzhou University's medical college Experimental Animal Center provides, and freely takes the photograph water and diet.

1.3 the preparation of reagent

Willow tea: pulverize the back and make thick extractum shape with water extraction, every gram is equivalent to crude drug 6.0 grams, preserves in the refrigerator of sealing back, with before adding the suspension that an amount of distilled water is made into desired concn.

Rhodiola rosea capsules: content taking-up in the capsule is dissolved in the distilled water, and the test dose of mice Radix Rhodiolae is 0.45g/kg.

2. test method

2.1 laboratory animal grouping

Mice is bought back breeding observing 3 days, eliminate body weight and motor capacity and average level and have behind the mice of notable difference residue male and female half and half totally 80 go into to organize.

Mice is divided into 5 groups at random, is respectively empty from matched group, Radix Rhodiolae group (positive control) and L (low), M (middle), three test group of H (high).Every group of 8 mices all carry out labelling with 5% fuchsin solution with semar technique, and mark is left front leg 1 in proper order, left lumbar region 2, left back lower limb 3,4, back of the body waist 5, tail base portion 6, RAT 7, right lumbar region 8.

2.2 dosage design

Experiment beginning mice average weight is counted 25g, and mice irritates initially that body of stomach is long-pending to be 0.25mL/ d, and body of stomach is long-pending does suitable adjustment to irritating with the actual increase situation of mice body weight later on.For trying mice after laboratory condition adapts to 3 days, 3 test group mices give L (low), M (middle), three kinds of various dose extract solutions of H (high) respectively, the Radix Rhodiolae group gives the Radix Rhodiolae aqueous solution, and the blank group only gives the equal-volume distilled water, every day 1 time.Its dosage designs as table 3:

The design of table 3 mice dosage

2.3 normal pressure hypoxia endurance test

Continuous irrigation stomach mice 21d, every group of 8 mices are irritated the stomach end after 1 hour at last, put into and fill 10g sodica calx (absorption moisture and carbon dioxide, the creation anaerobic environment) in the 250mL port grinding bottle (1 every bottle), bottleneck covers completely with all ten woodss sealings, the time of record white mice survival (with the standard of respiratory arrest as judgement death).

3. experimental result

Anoxia is a kind of stressor to body, influences the various metabolism of body, particularly influences the oxidation energy supply of body, finally can cause the major organs anoxia energy supplies such as the heart, brain of body not enough and dead.Experiment mice is strong more to anoxybiotic tolerance in the long more expression of following time-to-live of anaerobic environment.Experimental result such as table 4.

Table 4 willow tea is to the influence of mice normal pressure hypoxia-bearing capability

P＜0.05: compare from contrast that there were significant differences with sky; P＞0.05: compare no significant difference from contrast with sky;

Data show in the table 4, compare with blank, Radix Rhodiolae group and willow tea extract have all prolonged the time-to-live of mice in the normobaric hypoxia environment, and wherein Radix Rhodiolae and the MP group time-to-live of mice in the normobaric hypoxia environment obtained significant prolongation (P＜0.05).Compare between LP, MP, three test group of HP, three groups of mice time-to-live in the normobaric hypoxia environment do not have significant difference.This illustrates that high, medium and low dosage extract all can effectively improve the normal pressure hypoxia-bearing capability of mice, and three's effect does not have significant difference.

Above test shows that willow tea water extract can improve the exercise tolerance of mice body, and can obviously improve the liver/muscle glycogen content of mice body, and prompting willow tea has the obvious anti-fatigue effect; Simultaneously, experiment is proof also, and the willow tea extract can prolong mice in the following time-to-live of anaerobic environment, shows that willow tea has altitude sickness prevention, the effect of anti-hypoxia.

Claims (8)

1. Xianbei an ancient nationality in China spends the application in preparation antifatigue medicine.

2. the application of the water extract spent of Xianbei an ancient nationality in China in preparation antifatigue medicine.

3. Xianbei an ancient nationality in China spends the application in preparation antifatigue health product.

4. the application of the water extract spent of Xianbei an ancient nationality in China in preparation antifatigue health product.

5. Xianbei an ancient nationality in China spends the application in the medicine of preparation anti-hypoxia.

6. the application of the water extract spent of Xianbei an ancient nationality in China in the medicine of preparation anti-hypoxia.

7. Xianbei an ancient nationality in China spends the application in the health product of preparation anti-hypoxia.

8. the application of the water extract spent of Xianbei an ancient nationality in China in the health product of preparation anti-hypoxia.