CN102188500B - Traditional Chinese medicine preparation for treating anxiety neurosis - Google Patents
Traditional Chinese medicine preparation for treating anxiety neurosis Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine preparation for treating anxiety neurosis, prepared from the following medicinal raw materials in weight: 12g of bupleurum, 9g of scullcap, 12g of cassia twig, 30g of dragon bone, 30g of oyster, 1g of pearl, 15g of tuckahoe and 6g of rhubarb. The prescription takes the bupleurum as a monarch medicine for relieving shao yang; the scullcap is bitter in flavor and cold in property and can clear pathogenic heat to internally eliminate pathogenic heat from shao yang gallbladder, the cassia twig is pungent in flavor and warm in property and can activate obstructed yang, and the scullcap and the cassia twig are both ministerial medicines to play the efficacies of activating yang and expelling heat, and the monarch medicine and the ministerial medicines are matched for externally activating yang and internally expelling heat. The dragon bone, the oyster and the pearl powder are adjuvant medicines mainly effecting on the heart meridian and the liver meridian to play the efficacy of tranquilizing with heavy material; the tuckahoe has the efficacy of relieving mental stress and is good at clearing damp and promoting diuresis; and the rhubarb has the efficacies of expelling heat and harmonizing the stomach. All the medicines are combined to play the efficacies of tranquilizing mind, calming the liver to stop the wind, expelling fire in the gallbladder and stomach, activating qi of sanjiao, and benefiting urination and defecation to eliminate exopathogen by urination and defecation. The monarch medicine, the ministerial medicines and the adjuvant medicines supplement and complement each other by cold and warm treatment to play the efficacies of relieving depression of the liver and benefiting the gallbladder, harmonizing the exterior and interior, tranquilizing mind, and relieving complex pathogenic factors from the exterior and interior.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation of treating anxiety neurosis, belong to the field of Chinese medicines.
Background technology
Anxiety neurosis is claimed anxiety neurosis again; Be to be main clinical manifestation with generalized anxiety disorder (chronic anxiety) and ictal terrified state (acute anxiety); Often with dizzy, uncomfortable in chest, cardiopalmus, dyspnea, xerostomia, frequent micturition, urgent micturition, perspire, tremble and disease such as mobility's uneasiness; Its anxiety is not to be caused by actual threat, or its nervous terrified degree is very unbecoming with reality.Patient's performance anxiety, fear and intense strain feel that the worst thing is about to take place, and normal uneasy in either sitting or lying lacks the security, and all day is on tenterhooks, unsets, and things loses interest to external world.Frightened emotion is arranged when serious; Stimulate to external world and be prone to alarm response; Often with sleep disorder and autonomic nervous dysfunction phenomenon, like difficulty falling asleep, have nightmares, be prone to wake up with a start, pale complexion or flushing, be prone to perspire, extremity are numb, muscle beat, dizzy, cardiopalmus, chest have sense of compression or sensation of asphyxia, inappetence, xerostomia, abdominal part is swollen and burning sensation, constipation or diarrhoea, frequent micturition, menoxenia, hyposexuality etc. are arranged.
Chinese medicine is to the understanding of anxiety neurosis etiology and pathogenesis; Different times has different understanding, reaches common recognition basically but respectively cure house, thinks that promptly anxiety neurosis is mainly relevant with the consitution inherited genetic factors with external environment stimulation, internal injury caused by excess of seven emotions; Mainly be the imbalance of heart spleen hepatic and renal function, qi-blood disharmony forming.Yan Fuqing thinks that mostly its etiology and pathogenesis is that heresy is disturbed cardiopulmonary, gas the moon is impaired, and god keeps in not, strongly fragrant and heat-transformation, and all diseases of anxiety appear.Feng Zhenyu etc. think that its generation and the heart, liver, kidney three dirty functional disorders are relevant.Open to feel the people think disturb in the expectorant heat and make the gallbladder disalignment just be anxiety neurosis root also.The little strong grade of king thinks that heart spleen vim and vigour are not enough, and it is its basic pathogenesis that asthenic fire is disturbed the heart.Huang Yuedong thinks this sick position the heart, liver, and anxiety with the passing of time, can secretly consume cloudy blood, can cause hematogenous blockage.Zheng Zuyan etc. think that primary disease is many and cause that by being terrified suddenly or worried excessive thinking pathogenesis is the gas combination of phlegm and heat, hoodwink the mind, and cause irritability.Feng Qibin etc. think that from the beginning of with true, then Sthenia is transforming into asthenia in prolonged illness.Wang Zaitao thinks that primary disease and the heart, kidney are in close relations.Feng Hui thinks it because of feelings will onset, and is how dirty relevant with conscience spleen three.Chen Guangmin etc. think that then its cause of disease is lasting evil thought, and pathogenesis is the pathological change of refreshing vital essence, thereby exist a series of interdependent and growth and decline relation between the etiology and pathogenesis syndrome and can supply treatment utilization.Above-mentioned argumentation has all disclosed the characteristics of anxiety neurosis incidence and development from different perspectives.
In clinical treatment, ancient Chinese medicine doctor thinks that the variation of various cards can appear in disease along with course of disease progress, the dialectical pathological change that will disclose a certain moment essence in the disease progression process exactly, and it is a dynamic idea.Huang Yuedong divides three types with anxiety neurosis: insufficiency of heart-QI blood stasis type, the stagnant strongly fragrant type of phlegm fire of blood stasis type regulating liver-QI of the motive.Liu Zhengxue etc. think that many clinically anxiety neurosis with caused by hepatic stagnation qi stagnation and stagnation of liver-QI qi stagnation and blood stasis type are main.Zhang Chuanru thinks that the clinical pattern of syndrome of primary disease is simulataneous insufficiency and excessive, and the liver heart combination of syndromes, incoordination between the liver and stomach, insufficiency of the spleen stagnant, deficiency of kidney essence are respectively laid particular stress on.Yu Yihong reduces two types with it: simple type (deficiency of heart-yin causes nutrient blood to be lost) is mingled with type (the dyspepsia phlegm-fire stops in the stomach, disturbing the mind upwards).Sun Suming etc. think and are mostly stagnation of QI unconsciousness due to blockage of phlegm by this onset of disease, liver-spleen disharmony, and gods are become deaf, and are real how empty few: after the prolonged illness, deficiency of both QI and blood, cloudy smart consumption is hindered, and marrow brain cervical carcinoma with cachexia is empty how real few.Feng Hui then divides four types with it: disturb type, type of deficiency of heart-QI and gallbladder-QI and type of hyperactivity of fire caused by deficiency of YIN on caused by hepatic stagnation qi stagnation, the expectorant heat.
Record to primary disease also is dispersed in the ancient Chinese medicine doctor ancient books and records, like Xu Ling tire experience: " we are the frightened expectorant of liver and gall down, and controlling epilepsy must imitate ".Become the deteriorated case of disease involving both the exterior and the interior, deficient and excessive syndromes appearing together.Golden Mirror of Medicine was once called and " is to demonstrate,prove also, is the mixed heresy of negative and positive; The side of being also also attacks and mends mixed medicine.Bavin, Gui Xiewei pathogenic factor in the exterior to the greatest extent, Radix Et Rhizoma Rhei is attacked sunken interior-heat, Radix Ginseng, Rhizoma Zingiberis Recens, Fructus Jujubae tonify deficiency and stomach function regulating; Poria, Rhizoma Pinelliae diuretic and sending down the abnormal ascending QI, Os Draconis, Concha Ostreae, Plumbum preparatium puckery heavy, relieving convulsion getting into the frame of mind for work and calm the nerves brightly, this is for controlling mixed disease also with mixed medicine ", or the like.
Based on above theory, the object of the present invention is to provide the Chinese medicine preparation of the good treatment anxiety neurosis of a kind of therapeutic effect.
Summary of the invention
To above-mentioned prior art, the invention provides a kind of Chinese medicine preparation of treating anxiety neurosis.
On basis to TCM Document and modern study; The present invention is conscientiously summing up under the prerequisite that cures mainly symptom that generalized anxiety disorder emotion symptom and Radix Bupleuri adds Os Draconis Concha Ostreae soup; Through clinical constantly preferred, formed at last that to calm the nerves be that Therapeutic Principle's Radix Bupleuri adds the Os Draconis Concha Ostreae Tonga side of subtracting to discongest.Discongest tranquillization method and be inventor of the present invention a kind of method of treating anxiety reaction and generalized anxiety disorder that oneself is summed up in the theory of Chinese medical science framework, dirty based on the heart, liver two, method is a dispersing the stagnated live-QI to relieve the stagnation of QI, mind tranquilizing and the heart calming.Full side serves as main the composition with Radix Bupleuri, Ramulus Cinnamomi, Radix Scutellariae, Radix Et Rhizoma Rhei, Os Draconis, Concha Ostreae, Poria, Margarita etc.
The present invention realizes through following technical scheme:
A kind of Chinese medicine preparation of treating anxiety neurosis is processed by following bulk drugs: 10～15 parts of Radix Bupleuri, 6～12 parts of Radix Scutellariaes, 10～15 parts of Ramulus Cinnamomi, 25～35 parts of Os Draconis, 25～35 parts of Concha Ostreaes, 0.8～1.2 part of Margarita, 10～20 parts in Poria, 4～8 parts of Radix Et Rhizoma Rhei.
Preferably, process: Radix Bupleuri 12g, Radix Scutellariae 9g, Ramulus Cinnamomi 12g, Os Draconis 30g, Concha Ostreae 30g, Margarita 1g, Poria 15g, Radix Et Rhizoma Rhei 6g by following crude drug.
A kind of preparation technology who treats the Chinese medicine preparation of anxiety neurosis is characterized in that, may further comprise the steps:
(1) get Radix Bupleuri, Ramulus Cinnamomi, add 6 times of amounts (volume) water, soaked 1 hour, extract volatile oil then, extracted 5 hours, collect volatile oil, subsequent use;
(2) medicinal liquid of said extracted being crossed volatile oil filters, and must filter the back medicinal liquid, and is subsequent use; Medicinal residues and Radix Scutellariae mix, and extracting in water twice adds 8 times of amounts (volume) at every turn, extracts 2 hours at every turn, extracts after-filtration at every turn, must filtrate, and the filtrating of extracted twice and aforementioned filtration back combining medicine, being concentrated into relative density is 1.16 (60 ℃); In this concentrated solution, add ethanol then and reach 60% (60% is meant volume parts), left standstill 24 hours, filter, must contain alcohol filtrating to containing the alcohol amount, subsequent use;
(3) get Radix Et Rhizoma Rhei, add 6 times of amounts (volume), 70% ethanol (volume parts), twice of reflux, extract; The each extraction 2 hours extracted after-filtration at every turn, must filtrate; The alcohol filtrating that contains that obtains in filtrating and the step (2) merges, and reclaims ethanol to relative density and is 1.28～1.32 (50 ℃ of surveys), and is subsequent use;
(4) get Os Draconis, Concha Ostreae, Poria, add 8 times of water gagings and extract twice, extracted 2 hours at every turn, extract after-filtration at every turn, must filtrate, filtrating is concentrated into relative density and is 1.28～1.32 (50 ℃ of surveys) extractum, and is subsequent use;
(5) volatile oil is mixed by mass ratio 1:8 with beta-schardinger dextrin-, add 2 Bei β – CD water gagings (ml), ground 1 hour, 40 ℃ of dryings get the volatile oil beta-cyclodextrin inclusion complex, and are subsequent use;
(6) get Margarita, micronizing, subsequent use;
(7) extractum that the extractum that obtains in the step (3) and step (4) is obtained mixes, and adds Margarita micropowder, mix homogeneously, drying under reduced pressure below 70 ℃, dry extract, subsequent use;
(8) the volatile oil beta-cyclodextrin inclusion complex that obtains in the step (5) is mixed with the dry extract that step (7) obtains, pulverize, cross 80 mesh sieves, add the mixture of sucrose and dextrin, mix homogeneously is processed granule, dry getting final product below 60 ℃.
The mass ratio of sucrose and dextrin is 2:1 in the said step (8).
During treatment, oral, each 10g (being equivalent to crude drug amount 40.875g approximately), every day 2 times, 30 days one courses of treatment, cure general 1 course of treatment.
The side of prescription of the present invention separates as follows:
Radix Bupleuri: separate in the harmony in the exterior, dispersing the stagnated live-QI to relieve the stagnation of QI, ascending spleen-QI, effect such as Radix Bupleuri has antiinflammatory, protects the liver, analgesic, analgesia clinically is used to control few positive disease, has a headache menoxenia, dysmenorrhea due to the depression of liver-QI; The collapse of QI card.Diseases such as main treatment flu, gastric and duodenal ulcers, cholelithiasis, dysentery, bile reflux gastritis, yellow cellulitis hepatitis.Main component is a saikoside in the Radix Bupleuri root, and next contains plant sterol, adonite, and a small amount of volatile oil, polysaccharide.Aerial parts mainly contains flavonoid, small amounts of soap glycoside, lignanoids, Coumarins composition.Saikoside (saikosaponin) is one of wherein important active component, and mostly it is pentacyclic triterpene oleanane type derivant.Discover that saikoside has effects such as antiinflammatory, antiviral, antitumor, endocrine regulation and immune system.Radix Bupleuri also has clear and definite hepatoprotective effect, and the liver-protecting activity of saikoside mainly shows the following aspects: 1. reduce the activity of Cytochrome P450, protection hepatic necrosis, stimulated hepatic cell regeneration; 2. stimulate pituitary adrenal system, the originality glucocorticoid secretion increases in making; 3. reduce the activity of the coenzyme cytochrome C reductase of dehydrogenase, reduce the reaction of hormonelike side effect; 4. can make the giant cell activation, the generation of enhancing antibody, interferon; 5. promote albumen synthetic, increase hepatic glycogen, reduce lipid peroxide, promote liver cell regeneration; 6. the activity of enhanced NK cell and LAK.
Radix Scutellariae: classify as top gradely in the Shennong's Herbal, have heat clearing away, dampness, eliminating fire and detoxication, hemostasis, antiabortive effect.Modern pharmacological research, Radix Scutellariae have anti-HIV (pathogen of AIDS) activity, and no cytotoxicity and cell growth inhibiting phenomenon, become a kind of new effective prevention and the medicine of treatment AIDS.Radix Scutellariae still is a kind of good anti-ultraviolet material, has good blood pressure lowering in addition, suppresses superoxide radical to destruction, prevention and the treatment cataract of erythrocyte membrane and to the effects such as reverse of diabetic nephropathy.Radix Scutellariae mainly contains flavone compound, and wherein content of baicalin is the highest, is one of main effective ingredient.The pharmacological action of Radix Scutellariae mainly comes from baicalin wherein and can significantly alleviate acetaminophen and causes the GSH that hepatic injury causes and reduce; Reducing the liver lipid peroxidation thing simultaneously generates; Experiment shows that content of baicalin is lower than ethanol extract in the water extract, but the antiinflammatory of water extract, bacteriostasis all are better than ethanol extract.
Ramulus Cinnamomi: have relieving the exterior syndrome by diaphoresis, promoting the flow of QI-blood by warming the meridian, supporing yang activating QI, the effect of the flat gas that spins.Be used for anemofrigid cold, coldness and pain in the epigastrium, cold in blood amenorrhea, arthralgia, phlegm retention, edema, cardiopalmus, renal mass.These article contain volatile oil, and main component is a cinnamic aldehyde in the oil.Modern pharmacodynamic study shows: cinnamic aldehyde can the inhibition maincenter and show certain calmness, analgesia and refrigeration function.Oleum Cinnamomi is the armaticity carminative that is good for the stomach, and the intestines and stomach is had demulcent stimulation, can promote saliva and gastric secretion, strengthens digestive function, and can remove the visceral smooth muscle spasm, alleviates the intestinal spasmic pain.Cinnamic aldehyde can also suppress the formation of mice zest gastric ulcer, has prevention of arterial or venothrombotic effect, and arteria coronaria and cerebrovascular are had of short duration dilating effect, and peripheral blood vessel is had direct dilating effect, and certain leukocyte increasing and anti-radiation function are arranged.
Os Draconis: Os Draconis are mammals such as the skeleton fossil of Hippocampal cortex, rhinoceros class, deer class, bovine, type of elephant etc. or the fossil of type of elephant front tooth in ancient times.The property sweet-puckery flavor, flat.Has tranquillizing the mind by relieving convulsion, arresting sweating controlling nocturnal emission with astringent drugs, hemostasis astringing intestine to stop diarrhea, the effect of promoting tissue regeneration and ulcer healing.Cure mainly that to control infantile convulsion demented, palpitation with a distress feeling is forgetful, insomnia and dreamful sleep, and spontaneous sweating, the seminal emission stranguria with turbid discharge being told nosebleed and having blood in stool, bleeding not during menses, the dysentery proctoptosis, ulcer does not close up for a long time.Mainly contain calcium carbonate, calcium phosphate, still iron content, potassium, sodium, chlorine, sulfate radical etc.
Concha Ostreae: Concha Ostreae is the shell of the long Concha Ostreae Ostrea of Ostreidae animal gigas Thunberg, the curved Concha Ostreae Ostrea talienwhanensis Crosse in Dalian or Crassostrea rivularis Ostrea rivularis Gould.Nature and flavor are salty wet, cool.Have yin fluid astringing, YANG hyperactivity suppressing, hidroschesis, arresting seminal emission reduces phlegm, the effect of softening the hard mass.Be used for the palpitation with fear insomnia, vertigo and tinnitus, tuberculous lymphadenitis, abdominal mass mass in the abdomen, spontaneous sweating, seminal emission metrorrhagia and leukorrhagia, stomachache pantothenic acid.
Margarita: its nature and flavor are sweet, cool, and the calm the nerves arresting convulsion, the removing nebula that makes eye bright, yin nourishing of its function of successive dynasties book on Chinese herbal medicine record relieves dizziness, high fever, infantile convulsions, epilepsy, etc., and cure mainly palpitation with fear insomnia, infantile convulsion epilepsy, order life nebula, skin infection and disease such as do not hold back.Main chemical compositions is a calcium carbonate in the Margarita, also contains several amino acids, trace element and taurine.
Poria: Poria is to use time-honored Chinese medicine simply, and its property is flat, and sweet in the mouth, light has the effect of promoting diuresis to eliminate damp pathogen, invigorating the spleen and regulating the stomach, mind tranquilizing and the heart calming; The traditional Chinese medical science is mainly treated dysuria with it, edema, deficiency of spleen-QI and stomach-QI, anorexia and loose stool; Fatigue and asthenia and malaise, palpitation with fear, diseases such as insomnia.Modern pharmacology research shows that Poria has many-sided pharmacological actions such as tumor suppression, immunostimulant, antiinflammatory.Its main chemical compositions is polysaccharide and triterpene.
Radix Et Rhizoma Rhei: have many-sided drug effects such as heat-clearing and toxic substances removing, activating blood circulation to dissipate blood stasis, removing dampness hemostasis.Its complex chemical composition has kinds such as anthra-glucoside, stilbene glycosides, the glycosides of tanning, and wherein anthra-glucoside is main composition.Radix Et Rhizoma Rhei is one of important Chinese medicine, and is more to its research report.
In sum, full side is monarch with Radix Bupleuri, in order to discongest few sun; The Radix Scutellariae bitter cold is let out pathogenic heat clearly and can be made few positive gallbladder internal organs pathogenic heat internal diabetes, the hot temperature of Ramulus Cinnamomi, and sensible Yu Yang, the two closes and the merit of activating yang and expelling the heat-evil for the minister tool, and the monarch and his subjects are auxilliary mutually, let out in passing through inside.Assistant is with Os Draconis, Concha Ostreae, Margarita powder, main GUIXIN, liver two warps, the god's of lifting Zhenan County effect; Poria had both been had a merit of mind tranquilizing and the heart calming, was longer than promoting diuresis with drugs of tasteless flavour again, the Radix Et Rhizoma Rhei stomach function regulating that expels the heat-evil; All medicines share tranquilizing the mind, suppressing the hyperactive liver to relieve the wind syndrome, and the fire of catharsis gallbladder, stomach, the gas of sensible three warmers, sharp two just, makes evil descendingly just to go out from two.Supplement and complement each other in conjunction with monarch, minister, adjuvant drug, cold Wen Tongyong, depressed liver-energy dispersing and function of gallbladder promoting, inside and outside the reconciliation, tranquilizing the mind makes mixed evil must the separating of the exterior and the interior.
The specific embodiment
The present invention is further described with experiment below in conjunction with embodiment:
A kind of Chinese medicine preparation of treating anxiety neurosis of embodiment 1 preparation
Prescription is: Radix Bupleuri 12g, Radix Scutellariae 9g, Ramulus Cinnamomi 12g, Os Draconis 30g, Concha Ostreae 30g, Margarita powder 1g, Poria 15g, Radix Et Rhizoma Rhei 6g.
Method for preparing is following:
(1) get Radix Bupleuri, Ramulus Cinnamomi, add 6 times of amounts (volume) water, soaked 1 hour, extract volatile oil then, extracted 5 hours, collect volatile oil, subsequent use;
(2) medicinal liquid of said extracted being crossed volatile oil filters, and must filter the back medicinal liquid, and is subsequent use; Medicinal residues and Radix Scutellariae mix, and extracting in water twice adds 8 times of amounts (volume) at every turn, extracts 2 hours at every turn, extracts after-filtration at every turn, must filtrate, and the filtrating of extracted twice and aforementioned filtration back combining medicine, being concentrated into relative density is 1.16 (60 ℃); In this concentrated solution, add ethanol then and reach 60%, left standstill 24 hours, filter, must contain alcohol filtrating to containing the alcohol amount, subsequent use;
(3) get Radix Et Rhizoma Rhei, add 6 times of amounts (volume), 70% ethanol, twice of reflux, extract; The each extraction 2 hours extracted after-filtration at every turn, must filtrate; The alcohol filtrating that contains that obtains in filtrating and the step (2) merges, and reclaims ethanol to relative density and is 1.28-1.32 (50 ℃ of surveys), and is subsequent use;
(4) get Os Draconis, Concha Ostreae, Poria, add 8 times of water gagings and extract twice, extracted 2 hours at every turn, extract after-filtration at every turn, must filtrate, filtrating is concentrated into relative density and is 1.28～1.32 (50 ℃ of surveys) extractum, and is subsequent use;
(5) volatile oil is mixed by mass ratio 1:8 with beta-schardinger dextrin-, add 2 Bei β – CD water gagings (ml), ground 1 hour, 40 ℃ of dryings get the volatile oil beta-cyclodextrin inclusion complex, and are subsequent use;
(6) get Margarita, micronizing, subsequent use;
(7) extractum that the extractum that obtains in the step (3) and step (4) is obtained mixes, and adds Margarita micropowder, mix homogeneously, drying under reduced pressure below 70 ℃, dry extract, subsequent use;
(8) the volatile oil beta-cyclodextrin inclusion complex that obtains in the step (5) is mixed with the dry extract that step (7) obtains, pulverize, cross 80 mesh sieves; The mixture (mass ratio of sucrose and dextrin is 2:1) that adds sucrose and dextrin; Mix homogeneously is processed granule, dry getting final product below 60 ℃.
Main pharmacodynamics and Its Mechanisms thereof
1 pair of influence that overhead cross labyrinth rat behavior is learned: rat is bought the back adaptability and raises a week, by body weight, sex at random equilibrium be divided into blank, positive drug control group and receive totally 5 groups of the high, medium and low dose groups of test preparation.Positive controls gives diazepam 1mg/kg, receives three dose groups of test preparation with not receiving reagent thing 18g/kg, 9g/kg and 4.5g/kg.Irritate stomach by setting dosage with 10ml/kg; Successive administration 10 days; Test gets into open arms number of times (OE), gets into the open arms time (OT) behind the last administration 2h; Get into closure arm number of times (CE), get into the closure arm time (CT), probe into number of times downwards, behavioristics's indexs such as the upright number of times of closure arm back leg.And calculate respectively: get into the total degree (OE+CE) of open arms and closure arm, get into open arms number of times ratio (OE%), open arms time of staying ratio (OT%).The gained data are represented with average ± standard deviation.
Three dose groups all can significantly increase EPM rat OE% and OT% value as a result; Compare with matched group; High dose group OE ten CE are apparently higher than matched group (P < 0.01), and OE% and OT % also have tangible statistical significance (P < 0.05), and the OE% of middle dose groups and blank group relatively have the statistical significance (P < 0.01) of height; Other two indexs have increase, but with blank group there was no significant difference (P>0.05) relatively.Suppress rat in the rearing of closure arm value, but rat is not had the tangible trend that influences at the Head-dipping of open arms and platform area.Experimental result shows: this receives reagent in 4.5g/kg one 18g/kg dosage range; Has tangible anxiety effect; Consistent with the action direction of diazepam, from being influenced the result, EPM rat each item index sees, best with the 18g/kg dose effect; From the comprehensive evaluation analysis of each item index, it is relevant that its dose-effect relationship is positivity.Result such as table 1 are shown in 2.
Table 1 receive the reagent thing to the influence of EPM rat OE ten CE, OE%, OT% (
± S, n=10)
| Group | Dosage (g/kg) | OE+CE | OE% | OT% |
| Blank control group | —— | 10.78±2.80 | 30.02±10.48 | 34.06±11.58 |
| Diazepam | 0.001 | 13.25±2.62* | 43.60±13.35* | 43.08±13.37* |
| High dose | 18 | 14.00±2.24** | 42.09±11.94* | 46.84±16.59* |
| Middle dosage | 9.0 | 12.80±2.70 | 50.11±7.65** | 44.02±20.54 |
| Low dosage | 4.5 | 12.00±4.64 | 41.12±13.70* | 35.25±20.56 |
Annotate: with blank control group relatively " * " P 0.05, " * * " P < 0.01 (down with).
Table 2 receive the reagent thing to EPM rat Head-dipping, Rearing influence (
± S, n=10)
| Group | Dosage (g/kg) | Head-dipping | Rearing |
| Blank control group | —— | 21.33±3.77 | 14.67±3.84 |
| Diazepam | 0.001 | 19.38±6.80 | 11.38±3.72* |
| High dose | 18 | 19.33±8.69 | 13.78±6.06 |
| Middle dosage | 9.0 | 22.5±5.60 | 9.20±4.94** |
| Low dosage | 4.5 | 20.78±6.18 | 10.56±4.88* |
2 pairs of mice light and shades are worn the influence of case: 18-22gSPF level Kunming mouse, male and female half and half, by body weight, sex at random equilibrium be divided into the blank group; Diazepam 1.5mg/kg matched group; Receive reagent 26g/kg, 13g/kg and 6.5g/kg dose groups are mixed with respective concentration by the 20ml/kg gastric infusion; The blank group is given and co-content normal saline, continuous 7 days.After the last administration, animal is placed camera-lucida central authorities, the back of the body is observed These parameters 5min towards dividing plate.Write down number of times that mice passes dividing plate respectively and in time of camera-lucida and camera bellows.
The result shows that the antianxiety drugs diazepam is when 1.5mg/kg dosage; What increase mice wears the case number of times; Relatively have tangible significant difference (P < 0.05) with blank control group, show tangible angst resistance effect, but to there is not the significance influence in the time of camera-lucida; Its camera bellows time greater than the camera-lucida time, prompting possibly show certain syngignoscism.What receive that the reagent low dose group obviously increases mice wears the case number of times, relatively has tangible statistical significance (P < 0.05) with blank control group.Senior middle school's dose groups has increases the trend wear the case number of times, but with blank control group not statistically significant (P>0.05) relatively.But middle dose groups obviously increases the time of mice in camera-lucida, relatively has obvious statistical significance (P < 0.05) with the blank group, and the result is as shown in table 3.
Table 3 receive the reagent thing to light and shade wear case anxiety model mice influence (
± S, n=10)
| Group | Dosage (g/kg) | Number of times | The camera-lucida time | The camera bellows time |
| Blank control group | —— | 12.17±6.24 | 151.06±44.99 | 148.93±44.99 |
| Diazepam | 0.0015 | 17.75±6.98* | 138.25±52.55 | 161.75±52.55 |
| High dose | 26 | 16.25±6.14 | 145.51±42.11 | 154.49±42.11 |
| Middle dosage | 13 | 14.25±8.48 | 191.38±39.48* | 108.62±49.47* |
| Low dosage | 6.5 | 19.00±8.77* | 155.56±58.27 | 144.44±58.27 |
The influence of indexs such as 3 couples of rat vogel conflict and cerebral tissue 5-TH thereof: wistar rat; Male and female half and half; Buy the back adaptability and raise a week, prohibit to measure behind the water 24h and lick the waterside number, by lick waterside number, body weight and sex at random equilibrium be divided into blank group, model control group, diazepam 1.0mg/kg group and receive reagent 18g/kg, 9g/kg and three dose groups of 4.5g/kg; Totally 6 groups, 10 every group.Different dosing group medicine is mixed with respective concentration, irritates stomach with the 10ml/kg volume, and blank group and model control group are irritated the isometric normal saline of clothes.Each organized successive administration 10 days.After last administration, prohibiting water 48h, record punishment phase (3min) rat lick the waterside number.The test rat jugular vein blood sampling 2ml that finishes, the centrifugal 10min of 3000r/min, separation of serum carries out monoamine oxidase, MAO and measures, and blood sampling back rat takes off neck puts to death, and on ice pan, gets cerebral tissue rapidly, claims liquid nitrogen storage after the weight in wet base, carries out monoamine transmitters and detects.
The DZP group and the waterside number of licking that receives three dose groups of reagent all not show the conflict test angst resistance effect increase as a result; Opposite with expected results; Lick the waterside number average and be tangible downward trend between all groups of data show, and the antilinear that demonstrates dosage and drug effect concerns, be thought of as successive administration and make medicine in vivo due to the lasting caused sedative-hypnotic effect of valid density; The while data show; With the normal control group relatively, each is organized cerebral index and decreases, the diazepam group with receive that reagent is high, middle dose groups and normal group relatively have significant difference.Receive that low dose group obviously improves the MAO vigor in the reagent, corresponding with the content ratio of monoamine transmitters in the cerebral tissue, in the prompting, low dose group is to the influence of cerebral tissue monoamine transmitters maybe to influence the MAO vigor directly related with it.The effect and the dosage that receive reagent to reduce 5-TH content are inverse ratio, and its effect is consistent to the effect trend of MAO vigor with preparation, and result such as table 4 are shown in 5.
Table 4 receive the reagent thing to rat model lick waterside number and monoamine oxidase, MAO influence (
± S, n=10)
| Group | Dosage (g/kg) | Lick the waterside number | Cerebral index | The MAO vigor |
| Blank control group | —— | —— | 0.619±0.056 | 13.04±5.05 |
| Model group | —— | 384.2±71.19 | 0.589±0.056 | 8.67±5.23# |
| Diazepam | 0.001 | 224±113.65** | 0.565±0.058# | 9.48±8.10 |
| High dose | 18 | 235.5±56.20** | 0.549±0.059# | 8.88±5.27 |
| Middle dosage | 9.0 | 201.3±67.31** | 0.547±0.035# | 13.43±5.20* |
| Low dosage | 4.5 | 154.88±64.32** | 0.574±0.057 | 13.31±5.28* |
Annotate: compare with the blank group, " # " P 0.05, " ## " P < 0.01; Compare with model group, " * " P 0.05, " * * " P < 0.01.
Table 5 receive the reagent thing to the influence of monoamine neurotransmitters in the rat model brain (
± S, n=10)
| Group | Dosage (g/kg) | NE（ng/mg） | DA（ng/mg） | 5-HT（ng/mg） |
| Blank control group | —— | 2.612±0.823 | 1.19±0.160 | 0.571±0.116 |
| The model resistance | —— | 2.832±0.656 | 1.740±0.543## | 0.994±0.262## |
| Diazepam | 0.001 | 2.838±1.114 | 1.062±0.185** | 0.450±0.094** |
| High dose | 18 | 2.645±0.500 | 1.621±0.665# | 0.887±0.319## |
| Middle dosage | 9.0 | 2.792±0.622 | 1.756±0.536## | 0.751±0.175* |
| Low dosage | 4.5 | 2.15±1.553* | 1.307±0.314* | 0.642±0.238** |
Annotate: compare " # " P < 0.05 with the normal control group; Compare with model group, " * " P 0.05, " * * " P < 0.01.
Claims (4)
1. a Chinese medicine preparation of treating anxiety neurosis is characterized in that, is processed by following bulk drugs: 10～15 parts of Radix Bupleuri, 6～12 parts of Radix Scutellariaes; 10～15 parts of Ramulus Cinnamomi, 25～35 parts of Os Draconis, 25～35 parts of Concha Ostreaes; 0.8～1.2 part of Margarita powder, 10～20 parts in Poria, 4～8 parts of Radix Et Rhizoma Rhei.
2. a kind of Chinese medicine preparation of treating anxiety neurosis according to claim 1 is characterized in that, is processed by following crude drug: Radix Bupleuri 12g, Radix Scutellariae 9g, Ramulus Cinnamomi 12g, Os Draconis 30g, Concha Ostreae 30g, Margarita 1g, Poria 15g, Radix Et Rhizoma Rhei 6g.
3. the described a kind of preparation technology who treats the Chinese medicine preparation of anxiety neurosis of claim 1 is characterized in that, may further comprise the steps:
(1) get Radix Bupleuri, Ramulus Cinnamomi, add 6 times of water gagings, soaked 1 hour, extract volatile oil then, extracted 5 hours, collect volatile oil, subsequent use;
(2) medicinal liquid of said extracted being crossed volatile oil filters, and must filter the back medicinal liquid, and is subsequent use; Medicinal residues and Radix Scutellariae mix, and extracting in water twice adds 8 times of amounts at every turn, extracts 2 hours at every turn, extracts after-filtration at every turn, must filtrate, and the filtrating of extracted twice and aforementioned filtration back combining medicine, being concentrated into relative density is 1.16; In this concentrated solution, add ethanol then and reach 60%, left standstill 24 hours, filter, must contain alcohol filtrating to containing the alcohol amount, subsequent use;
(3) get Radix Et Rhizoma Rhei, add 6 times of amount 70% ethanol, reflux, extract, twice was extracted 2 hours at every turn, extracted after-filtration at every turn, must filtrate, and the alcohol filtrating that contains that obtains in filtrating and the step (2) merges, and reclaims ethanol to relative density and is 1.28～1.32, and is subsequent use;
(4) get Os Draconis, Concha Ostreae, Poria, add 8 times of water gagings and extract twice, extracted 2 hours at every turn, extract after-filtration at every turn, must filtrate, filtrating is concentrated into relative density and is 1.28～1.32 extractum, and is subsequent use;
(5) volatile oil is mixed by mass ratio 1:8 with beta-schardinger dextrin-, add 2 Bei β – CD water gagings, ground 1 hour, 40 ℃ of dryings get the volatile oil beta-cyclodextrin inclusion complex, and are subsequent use;
(6) get Margarita, micronizing, subsequent use;
(7) extractum that the extractum that obtains in the step (3) and step (4) is obtained mixes, and adds Margarita micropowder, mix homogeneously, drying under reduced pressure below 70 ℃, dry extract, subsequent use;
(8) the volatile oil beta-cyclodextrin inclusion complex that obtains in the step (5) is mixed with the dry extract that step (7) obtains, pulverize, cross 80 mesh sieves, add the mixture of sucrose and dextrin, mix homogeneously is processed granule, dry getting final product below 60 ℃.
4. a kind of preparation technology who treats the Chinese medicine preparation of anxiety neurosis according to claim 3 is characterized in that: the mass ratio of sucrose and dextrin is 2:1 in the said step (8).
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| CN105942517A (en) * | 2016-07-16 | 2016-09-21 | 惠安县科联农业科技有限公司 | Anxiety-improving health-care food and preparing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167629A (en) * | 1997-04-07 | 1997-12-17 | 刘一凡 | Non-sugar |
| CN1383867A (en) * | 2002-03-28 | 2002-12-11 | 山西桂龙医药有限公司 | Medicine for treating depression and its prepn |
| CN1985984A (en) * | 2006-12-14 | 2007-06-27 | 刘超 | Medicine composition for treating neurosis |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167629A (en) * | 1997-04-07 | 1997-12-17 | 刘一凡 | Non-sugar |
| CN1383867A (en) * | 2002-03-28 | 2002-12-11 | 山西桂龙医药有限公司 | Medicine for treating depression and its prepn |
| CN1985984A (en) * | 2006-12-14 | 2007-06-27 | 刘超 | Medicine composition for treating neurosis |
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| Title |
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| 刘荩文.增损柴胡加龙骨牡蛎汤的临床运用.《辽宁中医杂志》.1983,(第1期),26-27. * |
| 闫炳远.中医药结合心理疗法治疗焦虑症.《浙江中西医结合杂志》.2009,第19卷(第1期),24-25. * |
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