CN102175508A - Method for freezing and embedding tissue paraffin section - Google Patents
Method for freezing and embedding tissue paraffin section Download PDFInfo
- Publication number
- CN102175508A CN102175508A CN2010106098166A CN201010609816A CN102175508A CN 102175508 A CN102175508 A CN 102175508A CN 2010106098166 A CN2010106098166 A CN 2010106098166A CN 201010609816 A CN201010609816 A CN 201010609816A CN 102175508 A CN102175508 A CN 102175508A
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- embedding
- paraffin
- tissue
- section
- paraffin section
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Abstract
The invention discloses a method for freezing and embedding a tissue paraffin section. The method comprises the following steps of: placing a paper embedding frame impregnated with paraffin tissues and molten paraffin into a 4 DEG C refrigerating chamber, standing for 10 minutes, peeling off paraffin blocks from the packet-embedding frame, slicing and dyeing. Compared with the conventional cold water embedding method of the tissue paraffin section, the method disclosed by the invention is simple, fast, environment-friendly and practical; the paper embedding frame can be recycled; cold water is not required, and phenomenon such as the formation of a hollow because water enters the paraffin block is avoided; and the quality of the section is guaranteed. Moreover, the method is wide in application, can be used for tissue sections of animals and plants and pathological sections of human bodies.
Description
[technical field]
The refrigeration embedding method of tissue paraffin section de of the present invention relates to biology and pathology histotomy technical field, is a kind of tissue paraffin section de embedding methods simple and efficient, the practical characteristics of environmental protection that have.
[background technology]
Tissue paraffin section de is the most frequently used a kind of microtomy of tissues observed form and structure, is widely used in the diagnosis of bio-science research and clinical pathology.Its operating process generally comprises fixation of tissue, dehydration, transparent, waxdip, embedding, section, bonding die, exhibition sheet, dewaxing, rehydration, dyeing, dehydration, transparent, mounting.The method of embedding is normally poured the paraffin of fusing into papery embedding frame, treats that paraffin at the bottom one deck that condenses, puts into the tissue of waxdip, rectify the position, treat in the embedding frame wax top layer film that congeals into, rapidly the embedding frame is immersed in the cold water, after treating that wax stone cools off fully, from water, take out stand-by.
The weak point of this method is, one, papery embedding frame is disposable, can't utilize not enough environmental protection after the immersion again.Two, need to prepare cold water before the embedding, waste time and energy.Three, wax stone is intake easily, forms the cavity, causes into waste product, has a strong impact on the success or failure of chipping qualities and even experiment.
[summary of the invention]
Purpose of the present invention is intended to solve the deficiency of existing method, and a kind of refrigeration embedding method of tissue paraffin section de is provided, and this method is simple and efficient, environmental protection is practical.
For addressing the above problem, the invention discloses a kind of refrigeration embedding method of tissue paraffin section de.It is characterized in that the embedding step in the tissue paraffin section de manufacturing process: the tissue of waxdip will be housed and the papery embedding frame of the paraffin that dissolves, put into 4 ℃ of reefers of refrigerator, left standstill 10 minutes, from the embedding frame, strip out wax stone after, just can cut into slices, experimental procedure such as dyeing.
Advantage of the present invention is, one, because not immersion, and after wax stone stripped out, papery embedding frame can reuse, economic environmental protection.Two, need not to prepare cold water before the embedding, the embedding frame is put into 4 ℃ of reefers of refrigerator get final product, simple and efficient.Three, the phenomenon that the wax stone water inlet forms the cavity can not occur, chipping qualities is guaranteed.For the embedding frame of other material (as glass), also available this method in case the wax stone water inlet forms the cavity, causes into defective goods, has a strong impact on the success or failure of section effect and even experiment.
[embodiment]
Tissue paraffin section de of the present invention refrigeration embedding method is: the papery embedding frame of waxdip tissue and melted paraffin will be housed, put into 4 ℃ of reefers of refrigerator, leave standstill 10 minutes, from the embedding frame, strip out wax stone after, just can cut into slices, experimental procedure such as dyeing.
Embodiment:
Dissect the anoplophora glabripennis larva, take out intestinal tissue, divide two groups, fixing, dehydration, transparent, the waxdip step is all identical, embedding method adopts the cold water embedding method for one group, another group adopts refrigeration embedding method, ensuing section, bonding die, exhibition sheet, dewaxing, rehydration, dyeing, dehydration, transparent, the mounting step is all identical.
The present invention takes double-blind study to read sheet, and refrigeration embedding method and conventional cold water embedding method compare, section effect indifference, and the feasibility of this tissue paraffin section de refrigeration embedding method is definite.
Claims (1)
1. tissue paraffin section de refrigeration investment is mainly used in the embedding step in the tissue paraffin section de manufacturing process, it is characterized in that: the embedding frame of the paraffin of waxdip tissue and fusing will be housed, put into 4 ℃ of reefers of refrigerator, leave standstill 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106098166A CN102175508A (en) | 2010-12-28 | 2010-12-28 | Method for freezing and embedding tissue paraffin section |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106098166A CN102175508A (en) | 2010-12-28 | 2010-12-28 | Method for freezing and embedding tissue paraffin section |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102175508A true CN102175508A (en) | 2011-09-07 |
Family
ID=44518715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010106098166A Pending CN102175508A (en) | 2010-12-28 | 2010-12-28 | Method for freezing and embedding tissue paraffin section |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102175508A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300757A (en) * | 2015-09-22 | 2016-02-03 | 王善宗 | Quick and simple pathological frozen specimen manufacturing method |
| CN112697556A (en) * | 2020-12-25 | 2021-04-23 | 四川省农业科学院园艺研究所 | Method for preparing citrus plant root tip chromosome slices |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1916590A (en) * | 2005-08-15 | 2007-02-21 | 段增强 | Technique for fabricating label type biologic slice |
| CN101738338A (en) * | 2009-12-29 | 2010-06-16 | 李从善 | Method for manufacturing tissue array paraffin block |
-
2010
- 2010-12-28 CN CN2010106098166A patent/CN102175508A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1916590A (en) * | 2005-08-15 | 2007-02-21 | 段增强 | Technique for fabricating label type biologic slice |
| CN101738338A (en) * | 2009-12-29 | 2010-06-16 | 李从善 | Method for manufacturing tissue array paraffin block |
Non-Patent Citations (1)
| Title |
|---|
| 江虹: "制作石蜡切片的常见问题及处理", 《健康研究》, vol. 30, no. 5, 15 October 2010 (2010-10-15), pages 381 - 382 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300757A (en) * | 2015-09-22 | 2016-02-03 | 王善宗 | Quick and simple pathological frozen specimen manufacturing method |
| CN112697556A (en) * | 2020-12-25 | 2021-04-23 | 四川省农业科学院园艺研究所 | Method for preparing citrus plant root tip chromosome slices |
| CN112697556B (en) * | 2020-12-25 | 2024-02-23 | 四川省农业科学院园艺研究所 | Method for preparing citrus plant root tip chromosome tablet |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110907 |