CN101086004B - Enzyme method reverse extraction process for white fungus polysaccharidase - Google Patents
Enzyme method reverse extraction process for white fungus polysaccharidase Download PDFInfo
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- CN101086004B CN101086004B CN200710014978A CN200710014978A CN101086004B CN 101086004 B CN101086004 B CN 101086004B CN 200710014978 A CN200710014978 A CN 200710014978A CN 200710014978 A CN200710014978 A CN 200710014978A CN 101086004 B CN101086004 B CN 101086004B
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Abstract
The invention relates to a methof for extracting white fungus polysaccharide reversely. It comprises following steps: immersing white fungus in water, moderating pH to 7.3- 7.6, moving expanded white fungus to enzymolysis solution for enzymolysis for 1- 2hours, the temperature for enzymolysis is 35- 38 Deg. C, pH is 7.5- 8.5; moving white fungus to basic solution, immersing and heating to 90- 98 Deg. C for 0.5- 1.0 hour, stirring, removing foam residule on the surface, removing flocculated sluage, putting white fungus in acidic solution, immersing for 20 -40 minutes, removing flocculated sluage and putting it in light basic solution, immersing for 10- 20 minutes, moving it to clean water, taking it out, draining, dewatering, drying at low temperature an getting white fungus polysaccharide. The product can be used as terminal product to prevent fat absorption in intestine, reduce blood fat, prevent fatty liver, reduce weight, or used as main raw material to prepare health- care foodstuff that can reduce sugar content and prevent constipation.
Description
Technical field
The present invention relates to a kind of reverse extraction process of enzyme process of tremella polysaccharide.
Background technology
The extraction of the deep processing of white fungus and its elite composition is the white fungus plant husbandry urgent problem that promotes development.At present, white fungus is most of directly as food on the dining table and the edible frequently invigorant of people, but time-consuming when edible, inconvenience forms food custom, and directly ediblely also is difficult to bring into play the health-care effect that white fungus is prevented and cured diseases.The extracting method of existing tremella polysaccharide is after white fungus is made slurry and mixed with water, to isolate tremella polysaccharide again from mixed solution, and its shortcoming is a large amount of water consumptions and alcohol, and yield is very low, only is suitable for providing the usefulness of scientific research and testing, is difficult to form large-scale production.
Summary of the invention
The purpose of this invention is to provide a kind of reverse extraction process of enzyme process of tremella polysaccharide, the above-mentioned shortcoming that it can overcome prior art satisfies the requirement of scale operation.
The reverse extraction process of a kind of enzyme process of tremella polysaccharide is characterized in that white fungus is dipped in the water, regulates pH to 7.3-7.6, make the white fungus water-swelling, the white fungus after expanding is moved in the enzymolysis solution carry out enzymolysis, hydrolysis temperature is 35-38 ℃, pH7.5-8.5, time 1-2h shifts out the white fungus behind the enzymolysis from enzymolysis solution, place basic solution, soak after 10-20 minute, be warming up to 90-98 ℃, continue 0.5-1.0h, stir, remove the foam residues of liquid level emersion, cotton-shaped solid substance is shifted out, place acidic solution, soaked 20-40 minute, shift out cotton-shaped solid substance, place weak caustic solution, soaked 10-20 minute, move into clear water again, take out, drain, dehydration, cryodrying gets tremella polysaccharide; Described enzymolysis solution is the aqueous solution that contains pancreatin, and pH is 7.5-8.5.
The tremella polysaccharide that extracts by method of the present invention can absorb to stop enteron aisle lipid as end product separately, and blood fat reducing and prevention fatty liver, fat-reducing etc. also can be used as and mainly imitate preparation of raw material hypoglycemic, toxin-expelling and face nourishing, prevent protective foodss such as constipation.
Embodiment
Further specify the present invention below by embodiment.
The white fungus 100g that learnt from else's experience selected, impurity elimination and removed pallet is dipped in the water, uses Na
2CO
3The aqueous solution regulate pH to 7.3-7.6, make white fungus water-swelling 15 times to former weight, white fungus after expanding moved in the enzymolysis solution that contains pancreatin carry out enzymolysis, protein and lipoid material branch in the white fungus structure are explained, the concentration expressed in percentage by weight of pancreatin is 1.0-1.5% in the enzymolysis solution, hydrolysis temperature is 35-38 ℃, and pH is 7.5-8.5, time 1-2h.Shift out the white fungus behind the enzymolysis from enzymolysis solution, placing pH is the basic solution of 9-10, soaks 10-20 minute, begins to heat, be warming up to 90 ℃-98 ℃, continue 0.5-1.0h, stir, have foam residues to emerge this moment, and visible pearl-like Oil globule of microscopy and cell content relic are removed.From above-mentioned basic solution, cotton-shaped solid substance is shifted out then, place through regulate the acidic solution of pH to 6.0-6.5 with hydrochloric acid, soaked 20-40 minute, non-extraction organism of small molecules in the former white fungus and solubility in acid inorganic substance also are removed, this moment, this cotton-shaped solid substance presented glittering and translucent, White Dress apperance, shift out cotton-shaped solid substance, place the weak caustic solution of regulating pH to 7.3-7.5 through NaOH, soaked 10-20 minute, move into clear water again, take out after 10 minutes, drain dehydration, cryodrying, get tremella polysaccharide dry product 59g, wherein contain moisture 3.5%.After testing, this tremella polysaccharide purity reaches 98.2% (the sulfuric acid anthrone method is quantitative).
Described pancreatin is a natural product, wherein main trypsinase and the steapsin of containing.The tremella polysaccharide of using the present invention's production the experiment proved that: all be better than the tremella polysaccharide that current methods extracts at aspects such as hypoglycemic effect, the lipid absorption of prevention enteron aisle and strengthening immunity.
Claims (1)
1. the reverse extraction process of the enzyme process of a tremella polysaccharide is characterized in that white fungus is dipped in the water, regulates pH to 7.3-7.6, make the white fungus water-swelling, the white fungus after expanding is moved in the enzymolysis solution carry out enzymolysis, hydrolysis temperature is 35-38 ℃, pH7.5-8.5, time 1-2h shifts out the white fungus behind the enzymolysis from enzymolysis solution, place basic solution, soak after 10-20 minute, be warming up to 90-98 ℃, continue 0.5-1.0h, stir, remove the foam residues of liquid level emersion, cotton-shaped solid substance is shifted out, place acidic solution, soaked 20-40 minute, shift out cotton-shaped solid substance, place weak caustic solution, soaked 10-20 minute, move into clear water again, take out, drain, dehydration, cryodrying gets tremella polysaccharide; Described enzymolysis solution is the aqueous solution that contains pancreatin, and pH is 7.5-8.5; The pH of described basic solution is 9-10; The pH of acidic solution is 6.0-6.5; The pH of described weakly alkaline solution is 7.3-7.5; The concentration expressed in percentage by weight of pancreatin is 1.0-1.5% in the described enzymolysis solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710014978A CN101086004B (en) | 2007-06-21 | 2007-06-21 | Enzyme method reverse extraction process for white fungus polysaccharidase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200710014978A CN101086004B (en) | 2007-06-21 | 2007-06-21 | Enzyme method reverse extraction process for white fungus polysaccharidase |
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| CN101086004A CN101086004A (en) | 2007-12-12 |
| CN101086004B true CN101086004B (en) | 2010-05-19 |
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| CN200710014978A Expired - Fee Related CN101086004B (en) | 2007-06-21 | 2007-06-21 | Enzyme method reverse extraction process for white fungus polysaccharidase |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101257993B1 (en) * | 2010-09-13 | 2013-04-24 | 풀무원건강생활 주식회사 | Producing Method of extract of Tremella fuciformis using enzymes and the composition for improving memory strength comprising the extract. |
| CN102911280A (en) * | 2012-10-24 | 2013-02-06 | 江苏博杰特机电有限公司 | Preparation method of Tremelle fuciformis extract |
| CN105558523A (en) * | 2014-10-16 | 2016-05-11 | 浙江元康食品有限公司 | No-preservative white fungus thick soup and making technology thereof |
| CN105753999A (en) * | 2016-04-08 | 2016-07-13 | 广州市美驰化妆品有限公司 | Method for extracting tremella polysaccharide at ultralow temperature |
| CN108653316A (en) * | 2018-06-04 | 2018-10-16 | 清华大学 | Tremella polysaccharides are preparing the application in preventing antiobesity agents |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1071060A (en) * | 1991-09-25 | 1993-04-21 | 梁芳亭 | Auricularia auriculajudae and tremella mycelium acidic technique for production of proteoglycan |
| CN1778824A (en) * | 2004-11-25 | 2006-05-31 | 中国医学科学院放射医学研究所 | Multiple homogeneous tremella polysaccharide, its extraction and medicinal composition with this compound as active components |
| CN1807463A (en) * | 2005-01-18 | 2006-07-26 | 上海辉文生物技术有限公司 | White fungus heteropolysaccharide and its extract, preparation method and uses |
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- 2007-06-21 CN CN200710014978A patent/CN101086004B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1071060A (en) * | 1991-09-25 | 1993-04-21 | 梁芳亭 | Auricularia auriculajudae and tremella mycelium acidic technique for production of proteoglycan |
| CN1778824A (en) * | 2004-11-25 | 2006-05-31 | 中国医学科学院放射医学研究所 | Multiple homogeneous tremella polysaccharide, its extraction and medicinal composition with this compound as active components |
| CN1807463A (en) * | 2005-01-18 | 2006-07-26 | 上海辉文生物技术有限公司 | White fungus heteropolysaccharide and its extract, preparation method and uses |
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| JP特开平7-33623A 1995.02.03 |
| 崔蕊静 等.银耳多糖的提取及其在饮料中的应用.中国食用菌23 2.2004,23(2),39-41. |
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