CN100497651C - Sturgon chondrine extracting process - Google Patents
Sturgon chondrine extracting process Download PDFInfo
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- CN100497651C CN100497651C CNB2006101240667A CN200610124066A CN100497651C CN 100497651 C CN100497651 C CN 100497651C CN B2006101240667 A CNB2006101240667 A CN B2006101240667A CN 200610124066 A CN200610124066 A CN 200610124066A CN 100497651 C CN100497651 C CN 100497651C
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- chondrine
- sturgeon
- sturgon
- enzymolysis
- alkali
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Abstract
The process of extracting sturgeon chondrine includes the following steps: 1. taking sturgeon cartilage tissue, grinding and washing; 2. alkaline hydrolyzing sturgeon cartilage with alkali in 9-10 times sturgeon cartilage for 10-12 hr and regulating the pH value of the solution to neutrality; 3. enzymolyzing the alkaline hydrolyzed solution with enzyme in 0.5-1 wt% of sturgeon cartilage at 40+/-2 deg.c for 7-8 hr; and 4. adding 2-3 times alcohol solution in 95 % concentration to the enzymolyzed solution via stirring, stilling at 0-4 deg.c for 8-10 hr, eliminating supernatant, stoving the precipitate in a stove at 40-50 deg.c to obtain sturgeon chondrine product. The process has high sturgeon chondrine yield and quality.
Description
Technical field
The present invention relates to a kind of Sturgon chondrine extracting process.
Background technology
Sturgeon also claims the sturgeon dragon, and the same with shark is the most ancient and primary cartilage fingerling on the earth, is the fish of individual maximum, longest-lived in the freshwater fish, has very high economic worth and scientific value.Along with the success and the popularization of sturgeon artificial propagation and cultural technique, China's sturgeon cultivation had been obtained significant achievement already, cultured area of sturgeon and cultured output cumulative year after year.The fish chrondroitin is one of hot issue of researchist's concern in recent years.The sturgeon entire body is a cartilage, has anticancer and calcium supplementing effect, it is reported that the chondroitin sulfate cellulose content of cartilage in sturgeon head, notochord, the fin up to 30%, have saying of " shark fin, sturgeon bone, the establishing-Yang that makes eye bright of food is promoted longevity ".
Chondroitin sulfate (Chondroitin sulfate, be called for short Chs) be the natural acidic mucopolysaccharide that from the animal cartilaginous tissue, extracts, multiple isomer such as ChsA, ChsB, ChsC, ChsD and ChsE are arranged, they all are the disaccharide polymkeric substance that alternately linked by D-glucuronic acid and N-acetyl-D-amino semi-lactosi, just sulfate group position difference.The β elimination reaction takes place in its common and proteic form existence of protein bound saccharogenesis when its hydrolysis, connect the O-glycosidic bond fracture of Serine hydroxyl in chondroitin sulfate and the protein, can obtain the chondroitin sulfate of free state.Chondroitin sulfate is white or micro-yellow powder, has water absorbability, and the bigger solution of viscosity that forms soluble in water is insoluble in organic solvents such as methyl alcohol, ethanol, ether, propyl alcohol, acetone and Glacial acetic acid.Hydrolysis easily takes place and comes off in chondroitin sulfate case of thermal instability, ethanoyl, and more facile hydrolysis becomes the oligose of different polymerization degree under acidic conditions.In recent years, along with to the going deep into of its physiological function and biochemical property research, find that Chs has anti-freezing, anti-inflammatory, antithrombotic, anticancer and reduce myocardial consumption of oxygen, promote coronary artery circulation, reducing blood-fat, anticoagulation and prevent effect such as arteriosclerosis; Treatment to cardiovascular disordeies such as coronary heart disease, atherosclerosis, stenocardia, myocardial anoxia, myocardial infarctions has certain curative effect; Also neurocyte, mucomembranous cell, nephrocyte etc. had provide protection; Chs also can activate lipolytic enzyme, makes fat acid decomposition, is to prevent fat active substance therefore; Chs can become the fitness of used for cosmetic in protection skin with some substance modulation; Chs also has water-retentivity, protects colloidality and high viscosity, and the Chs that China exports to Japan, Korea S is used as foodstuff additive.Therefore Chs is the important source material of domestic and international popular healthcare products and makeup and pharmaceuticals.Therefore study the extraction of Sturgon chondrine, can make full use of the sturgeon cartilage resource, not only can improve the sturgeon added value of product, and minimizing is to the pollution of environment, also can develop the sturgeon series product, to promoting further developing of China's sturgeon cultivation industry, make the breed of China sturgeon and be processed to form the inexorable trend that benign cycle is development, have immeasurable meaning for the economy and the social value that improve sturgeon.
Summary of the invention
The object of the present invention is to provide Sturgon chondrine extracting process, enzymolysis is combined with alkaline hydrolysis technology, optimize the extraction process of Sturgon chondrine, improve the extraction yield and the quality of Sturgon chondrine, promote the lifting of its economic benefit.
For achieving the above object, the invention provides a kind of Sturgon chondrine extracting process, specifically comprise the steps:
May further comprise the steps:
A, get the sturgeon cartilage tissue, blend and rinse well;
B, the above-mentioned sturgeon cartilage that blends is carried out alkaline hydrolysis, the alkaline hydrolysis time is 10～12h,, consumption is 9～10 times of sturgeon cartilage weight; Transfer pH to neutral solution behind the alkaline hydrolysis;
C, then carry out enzymolysis to transferring to the neutral alkali solution liquid, enzymolysis time 7～8h, the enzyme amount is 0.5～1% of a sturgeon cartilage weight, 40 ± 2 ℃ of hydrolysis temperatures;
D, the solution behind the enzymolysis is added 95% ethanol by the volume ratio of 1:2～1:3, stir with glass rod while adding; Staticly settle 8～10h in 0～4 ℃ then, remove supernatant liquor, the gained throw out with 40～50 ℃ of oven dry, obtains the Sturgon chondrine product in baking oven.
Used alkali kind is NaOH among the described step b, and alkali concn is 5.5～6.5%.
Used enzyme is a pancreatin among the described step c, and enzyme activity is 180 ± 20U.
Can reach following effect after adopting the present invention: the present invention combines alkaline hydrolysis with enzymolysis process, promotes that protein decomposes in the cartilaginous tissue, and effectively the bonded chrondroitin discharges with it, improves the extraction yield and the quality product of Sturgon chondrine.Product colour is an oyster white, and viscosity is higher, is the blob of viscose shape, and thick product yield is about 16%, and wherein the high-content of amidohexose reaches 27.58%.
Embodiment
Embodiment 1
Sturgeon cartilage after cleaning is carried out alkaline hydrolysis, and the alkali kind is Na0H, and alkali concn is 5.5%, and alkaline hydrolysis time 12h, alkali lye consumption are about 10 times of sturgeon cartilage weight; Transfer pH to neutral solution behind the alkaline hydrolysis.To transfer to the neutral alkali solution liquid and carry out enzymolysis with pancreatin, enzyme activity is 200U, and enzyme dosage is a sturgeon cartilage weight 1.0%, 40 ± 2 ℃ of hydrolysis temperatures, enzymolysis time 8h.Solution behind the enzymolysis is pressed 1:2.5 (v/v) add 95% ethanol, stir gently with glass rod while adding.Staticly settle 9h in 0～4 ℃ then, carefully topple over the removal supernatant liquor, with 45 ℃ of oven dry, it is faint yellow obtaining the Sturgon chondrine product to throw out in baking oven, and thick product yield is 16.20%, and wherein the content of amidohexose is 27.58%.
Embodiment 2
Sturgeon cartilage is carried out alkaline hydrolysis, and the alkali kind is NaOH, and alkali concn is 6%, and alkaline hydrolysis time 11h, alkali lye consumption are about 9.5 times; Transfer pH to neutral solution behind the alkaline hydrolysis.To transfer to the neutral alkali solution liquid and carry out enzymolysis with pancreatin, enzyme activity is 190U, and enzyme dosage is 0.75%, 40 ± 2 ℃ of hydrolysis temperatures, enzymolysis time 7.5h.Solution behind the enzymolysis is pressed 1:3 (v/v) add 95% ethanol, stir gently with glass rod while adding.Staticly settle 10h in 0～4 ℃ then, carefully topple over the removal supernatant liquor, throw out in baking oven with 50 ℃ of oven dry, obtaining Sturgon chondrine product product colour is milk yellow, and viscosity is higher, is the blob of viscose shape, thick product yield is 15.58%, and wherein the content of amidohexose is 26.31%.
Embodiment 3
Sturgeon cartilage is carried out alkaline hydrolysis, and the alkali kind is NaOH, and alkali concn is 6.5%, and alkaline hydrolysis time 10h, alkali lye consumption are about 9 times; Transfer pH to neutral solution behind the alkaline hydrolysis.To transfer to the neutral alkali solution liquid and carry out enzymolysis with pancreatin, enzyme activity is 180U, and enzyme dosage is 0.5%, 40 ± 2 ℃ of hydrolysis temperatures, enzymolysis time 8h.Solution behind the enzymolysis is pressed 1:2 (v/v) add 95% ethanol, stir gently with glass rod while adding.Staticly settle 8h in 0～4 ℃ then, carefully topple over the removal supernatant liquor, throw out in baking oven with 40 ℃ of oven dry, obtaining Sturgon chondrine product product colour is milk yellow, and viscosity is higher, is the blob of viscose shape, thick product yield is 16.30%, and wherein the content of amidohexose is 23.66%.
Claims (3)
1, a kind of Sturgon chondrine extracting process is characterized in that may further comprise the steps:
A, get the sturgeon cartilage tissue, blend and rinse well;
B, the above-mentioned sturgeon cartilage that blends is carried out alkaline hydrolysis, the alkaline hydrolysis time is 10～12h, and consumption is 9～10 times of sturgeon cartilage weight, transfers pH to neutral solution behind the alkaline hydrolysis;
C, then carry out enzymolysis to transferring to the neutral alkali solution liquid, enzymolysis time 7～8h, enzyme dosage are 0.5～1% of sturgeon cartilage weight, 40 ± 2 ℃ of hydrolysis temperatures;
D, the solution behind the enzymolysis is added 95% ethanol by the volume ratio of 1:2～1:3, stir with glass rod while adding; Staticly settle 8～10h in 0～4 ℃ then, remove supernatant liquor, the gained throw out with 40～50 ℃ of oven dry, obtains the Sturgon chondrine product in baking oven.
2, Sturgon chondrine extracting process according to claim 1 is characterized in that: used alkali kind is NaOH among the described step b, and alkali concn is 5.5～6.5%.
3, Sturgon chondrine extracting process according to claim 1 is characterized in that: used enzyme is a pancreatin among the described step c, and enzyme activity is 180 ± 20U.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101240667A CN100497651C (en) | 2006-12-05 | 2006-12-05 | Sturgon chondrine extracting process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101240667A CN100497651C (en) | 2006-12-05 | 2006-12-05 | Sturgon chondrine extracting process |
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| CN1974778A CN1974778A (en) | 2007-06-06 |
| CN100497651C true CN100497651C (en) | 2009-06-10 |
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| CNB2006101240667A Expired - Fee Related CN100497651C (en) | 2006-12-05 | 2006-12-05 | Sturgon chondrine extracting process |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101220383B (en) * | 2008-01-18 | 2010-06-02 | 中国水产科学研究院南海水产研究所 | Method for producing calcium amino acid |
| CN103130915B (en) * | 2012-03-31 | 2015-09-02 | 大连工业大学 | The method of chondroitin sulfate is prepared based on fish cranial cartilage |
| CN102924624A (en) * | 2012-11-26 | 2013-02-13 | 中国水产科学研究院南海水产研究所 | Method for preparing chondroitin sulfate from sturgeon cartilage |
| CN103554304B (en) * | 2013-11-07 | 2015-07-22 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for preparing low-molecular-weight sturgeon chondroitin sulfate by utilizing sturgeon chine |
| CN104788586B (en) * | 2015-03-31 | 2018-03-09 | 南方科技大学 | Chondroitin sulfate strontium and preparation method thereof |
| CN106432541A (en) * | 2016-09-19 | 2017-02-22 | 福建中医药大学 | Method for extracting sturgeon cartilage extract |
| CN107964055A (en) * | 2016-10-19 | 2018-04-27 | 清华大学 | Giant salamander cartilage chondroitin sulfate and its extracting method |
-
2006
- 2006-12-05 CN CNB2006101240667A patent/CN100497651C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 硫酸软骨素快速提取法研究. 罗曼，蒋立科.动物学杂志，第35卷第5期. 2000 |
| 硫酸软骨素快速提取法研究. 罗曼，蒋立科.动物学杂志，第35卷第5期. 2000 * |
| 硫酸软骨素的提取合分离纯化技术. 李鑫等.天然产物研究与开发，第16卷第6期. 2004 |
| 硫酸软骨素的提取合分离纯化技术. 李鑫等.天然产物研究与开发，第16卷第6期. 2004 * |
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