CN102171333A - 培养细胞的方法 - Google Patents

培养细胞的方法 Download PDF

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Publication number
CN102171333A
CN102171333A CN2009801393452A CN200980139345A CN102171333A CN 102171333 A CN102171333 A CN 102171333A CN 2009801393452 A CN2009801393452 A CN 2009801393452A CN 200980139345 A CN200980139345 A CN 200980139345A CN 102171333 A CN102171333 A CN 102171333A
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CN
China
Prior art keywords
cells
microcarriers
culture
cell
cell culture
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Pending
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CN2009801393452A
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English (en)
Chinese (zh)
Inventor
B·V·卡拉
R·Y·博迪
A·奈特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Diosynth Biotechnologies UK Ltd
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Avecia Biologics Ltd
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Application filed by Avecia Biologics Ltd filed Critical Avecia Biologics Ltd
Publication of CN102171333A publication Critical patent/CN102171333A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Dermatology (AREA)
  • Virology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2009801393452A 2008-08-07 2009-08-06 培养细胞的方法 Pending CN102171333A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0814443.8A GB0814443D0 (en) 2008-08-07 2008-08-07 Process for cultivating cells
GB0814443.8 2008-08-07
PCT/GB2009/001938 WO2010015831A2 (en) 2008-08-07 2009-08-06 Process for cultivating cells

Publications (1)

Publication Number Publication Date
CN102171333A true CN102171333A (zh) 2011-08-31

Family

ID=39767656

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801393452A Pending CN102171333A (zh) 2008-08-07 2009-08-06 培养细胞的方法

Country Status (7)

Country Link
US (1) US20110312089A1 (enExample)
EP (1) EP2318517A2 (enExample)
JP (1) JP2011529701A (enExample)
KR (1) KR20110042210A (enExample)
CN (1) CN102171333A (enExample)
GB (1) GB0814443D0 (enExample)
WO (1) WO2010015831A2 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0916370D0 (en) * 2009-09-18 2009-10-28 Avecia Biolog Ltd Compositions
WO2017044158A1 (en) * 2015-09-07 2017-03-16 Bioreactor Sciences Llc Method of continuous mass production of progenitor stem-like cells using a bioreactor system
IL258738B (en) * 2018-04-16 2020-04-30 Pluristem Ltd Methods and compositions for formulating and dispensing pharmaceutical formulations
CN110093312A (zh) * 2019-05-15 2019-08-06 张永国 一种细胞规模化培养方法、纯化方法及细胞信使
KR102111283B1 (ko) 2019-12-12 2020-05-15 주식회사 이뮤니스바이오 면역세포의 진탕 배양 방법

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050089512A1 (en) * 2001-12-19 2005-04-28 Kordula Schlotmann Skin/hair equivalent with reconstructed papillae
US7819934B2 (en) * 2006-07-14 2010-10-26 Xcellerex, Inc. Environmental containment systems

Also Published As

Publication number Publication date
US20110312089A1 (en) 2011-12-22
WO2010015831A2 (en) 2010-02-11
EP2318517A2 (en) 2011-05-11
GB0814443D0 (en) 2008-09-10
JP2011529701A (ja) 2011-12-15
WO2010015831A3 (en) 2010-04-01
KR20110042210A (ko) 2011-04-25

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Application publication date: 20110831