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  • C12N5/06—Animal cells or tissues; Human cells or tissues
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  • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
  • C12N5/0627—Hair cells
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  • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
  • C12N5/06—Animal cells or tissues; Human cells or tissues
  • C12N5/0602—Vertebrate cells
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  • C12N5/0656—Adult fibroblasts
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  • C12N2531/00—Microcarriers

Definitions

• the present invention concerns a process for cultivating differentiated human cells retaining stem cell potential.
• Stem cells and cells having stem cell potential are of increasing interest in many therapeutic areas. Such cells are typically produced by cultivation of cells anchored to a solid support medium.
• Mouse embryonic stem cells have been shown to grow in stirred suspension culture on Sigma SoIoHiII (Fibronectin-coated polystyrene) and Cytodex 3 (collagen) microcarriers. (Fok EY et al, Stem Cells 2005, 23(9): 1333-42).
• Porcine bone marrow-derived primary mesenchymal stem cells have been shown to grow on Cytodex type 1 , 2 and 3 (collagen) microcarriers (Frauschuh S et al, Biotechnol Prog. 2007, 23(1): 187-193).
• Embryonic feline lung fibroblasts have been shown to grow on Cytodex 1 in wave and stirred tank bioreactors (Hundt B et al, Vaccine 2007, 25(10): 3987-95).
• Mouse embryonic stem cells have also been shown to grow on Cytodex 3 microcarriers in spinner flasks (Abranches E et al, Biotechnol Bioeng. 2007, 96(6): 1211-21).
• it was discovered that such microcarriers suffer from a number of deficiencies rendering them unsuitable for reliable and scaleable production of differentiated human cells retaining stem cell potential.
• US2007/0264713 discloses that many different microcarriers can be employed to grow many different types of stem cells.
• a gelatin microcarrier is exemplified along with a collagen microcarrier in the cultivation of human embryonic stem cells. The results show that the collagen microcarrier gives an increase in cells three times greater then the gelatin microcarrier.
• a process for cultivation of differentiated human cells retaining stem cell potential which comprises culturing differentiated human cells retaining stem cell potential anchored to a microcarrier selected from the group consisting of gelatin microcarriers and quaternary ammonium derivatised polystyrene microcarriers.
• the cells are anchored to the microcarrier by methods known in the art, for example including by attachment to the surface of the microcarrier, by attachment to the internal structure of macroporous microcarriers, or by physical entrapment inside the internal structure of macroporous microcarriers.
• Gelatin microcarriers which can be employed in the method of the present invention can be composed of gelatin particles, cross linked gelatin particles or gelatin used as a coating on carrier materials, for example polystyrene or glass particles.
• the gelatin can be of natural source or recombinantly or synthetically produced.
• Gelatins or collagens can be crosslinked via the amine groups of lysine, via carboxyl groups glutamic acid or aspartic acid, or a combination thereof.
• Gelatin microcarriers are typically approximately spherical but can have other shapes and can be either porous or solid. Both porous and solid types of microcarriers are commercially available. Microcarriers may have a dense surface with dents to facilitate anchorage of the cells.
• Macroporous gelatin microcarriers for example are available commercially from Percell Biolytica AB, Sweden under the tradename Cultispher, especially Cultispher G and Cultispher S.
• Gelatin macroporous microcarriers are typically comprise particles based on a highly cross linked gelatin matrix, often with a particle size of from 10-500 ⁇ m and form a polymer matrix enclosing a large number of cavities typically having a diameter of from 1-50 ⁇ m.
• the particle size range of the microcarrier is commonly selected to be large enough to accommodate the anchorage of the stem cell whilst being small enough to form suspensions with properties suitable for use in cell culture bioreactors such as shake flasks, roller bottles, spinner flasks, wave bioreactors and stirred tank bioreactor systems.
• Quaternary ammonium derivatised polystyrene microcarriers which can be employed in the present invention comprise and amino-group attached to polystyrene, the amino group being quatemised, preferably by three alkyl groups, especially three C 1-4 alkyl groups. Examples of such microcarriers include HyQSphereTM HLX11-170, commercially available from Hyclone/ThermoFisher Scientific Inc.
• Gelatin microcarriers are particularly suited for applications where cells are cultured on different batches of microcarriers, where cells are cultivated on a first batch of microcarrier, and then to expand the scale of cultivation, cells are harvested and then reattached, optionally after storage for a period, for example storage in a freezer, to a microcarrier, typically a larger batch of microcarrier, or to multiple cultivators totalling a larger amount of microcarrier. Such culturing - harvesting and reattachment can be repeated as often as necessary to produce the desired quantity of cells.
• Gelatin microcarriers can be employed as the initial microcarrier or as subsequent microcarriers, or an alternative microcarrier, especially a quaternary ammonium-derivatised polystyrene microcarrier, can be employed as the initial microcarrier, with subsequent reattachment post harvesting being to a gelatin microcarrier.
• Quaternary ammonium-derivatised polystyrene microcarriers are suited to applications where the cells are seeded onto the microcarrier and cultured on that microcarrier until harvest, with no detachment and reattachment, or as the initial microcarrier with subsequent harvesting and reattachment to a gelatin microcarrier. Additionally, a quaternary ammonium-derivatised polystyrene microcarrier may be employed as the final microcarrier, with other microcarriers being employed for earlier cultivation steps.
• Differentiated human cells retaining stem cell potential which can be produced by the method of the present invention are known in the art, and are preferably adult cells, especially mesenchymal cells, and most preferably dermal cells, including adiposyte cells.
• Especially preferred cells include dermal sheath cells, dermal fibroblast cells and dermal papilla cells, especially dermal sheath cells as described in EP-A-0980270, dermal papilla cells as described in US5851831 and most especially dermal fibroplast cells as described in co-pending British patent application no. 0913469.3.
• the process according to the present invention can be carried out in vessels known in the art, including tissue culture flasks, shake flasks, spinner flasks, stirred tank bioreactors, disposable bag based bioreactor systems such as wave cell culture systems, and expanded bed bioreactor systems.
• vessels known in the art including tissue culture flasks, shake flasks, spinner flasks, stirred tank bioreactors, disposable bag based bioreactor systems such as wave cell culture systems, and expanded bed bioreactor systems.
• Options for large scale production also include roller bottles, hollow fibre systems, single, multi-plate or stacked-plate culture systems and cell cubes.
• the process of the present invention is commonly carried out by cultivating the cells in a stirred tank culture vessel system.
• the cells, microcarriers and nutrient medium are supplied to the culture vessel and stored under conditions conducive to cell propagation. If desired, additional culture medium may be added to the culture vessel system until the culture is finally terminated and cells harvested.
• the process of the present invention is carried out by cultivating the cells under conditions conducive to the growth of the cells whilst retaining the cell's stem cell potential.
• Culture conditions such as temperature, pH, dissolved oxygen (including hypoxic low oxygen conditions) and the like, are those known to be optimal for the particular cell and will be apparent to the skilled person (see, e.g., Animal Cell Culture: A Practical Approach 2 nd Ed., Rickwood, D.
• cells are cultured at a pH around neutral pH, commonly in the range of from 6.5 to 7.5 and a temperature in the range of from about 30 to 38°C.
• the cells are cultured in a vessel which is agitated with a with an agitator comprising two or more impellers, commonly rotational stirrers, located at different depths in the culture vessel.
• the impellers are mounted about a common shaft.
• each impeller is preferably located towards the base of the medium in the culture vessel, such as in the lower third of the vessel, with the second impeller located either towards the middle of the medium in the vessel, such as in the middle third, or towards the top of the medium in the vessel, such as in the top third.
• each impeller comprises two, three of four blades angled compared with the axis of the impeller shaft(s), such as propeller-type blades.
• the stirrers are selected to have a stirrer diameter : vessel diameter ratio of at least 0.25 : 1 , such as in the range from 0.3 : 1 to 0.7 : 1.
• the cells are cultured in a vessel which is agitated with a with an agitator comprising at least one helical blade, and preferably a helical stirrer as described in International patent application WO00/66258.
• the process is operated in one culture vessel, the cells are inoculated directly into the culture vessel containing microcarriers, the cells are propagated until the desired cell density is reached and the cells harvested.
• the process is operated in at least two distinct cell culture vessels. Cultivation may take place in one or more seed expansion vessels, followed by cultivation in a cell production vessel, and from which the cell product is harvested.
• the multiple seed expansion process may take in culture vessels of increasing size until a sufficient number of cells is obtained for the inoculation of the final production cell culture vessel.
• the seed expansion culture vessels can be of the same type (e.g. tissue culture flasks, shake flasks, roller bottles, spinner flasks, wave bioreactors, stirred tank bioreactors) but increasing in size as the seed expansion progresses or can be a mixture of culture systems increasing in size as the seed culture is expanded in readiness for transfer to the production bioreactor (e.g. tissue culture flasks to shake flasks to spinner flasks to stirred tank bioreactor systems, etc).
• fed batch or continuous cell culture conditions are devised to enhance growth of the cells in culture.
• Culture conditions such as temperature, pH, dissolved oxygen (d ⁇ 2 ) and the like, are those used with the particular cell and will be apparent to the ordinarily skilled artisan.
• the pH is adjusted to a level between about 6.5 and 7.5 using either an acid (e.g., CO 2 ) or a base (e.g., Na 2 CO 3 or NaOH).
• a suitable temperature range for culturing cells is often between about 30° to 38°C and a suitable d ⁇ 2 is often between 5-90% of air saturation.
• Cells can be released from gelatin microcarriers using procedures known in the art which limit the potential damage to the cell harvest during cell recovery processes.
• Cells are commonly released from gelatin microcarriers with the aid of a proteolytic enzyme, for instance, collagenase. Subsequent to the termination of the growth of the cells, the cells are released, when desired, from the carrier with such a proteolytic enzyme.
• the medium exchange can be performed by allowing the microcarriers to settle to the bottom of the cell culture vessel, after which a selected percentage of the cell culture growth medium volume is removed and a corresponding percentage of fresh cell culture growth medium is added to the cell culture vessel. The microcarriers are then re- suspended in the medium and this process of medium removal and replacement are typically repeated.
• 'Cell culture generally refers to cells taken from a living organism and grown under controlled conditions.
• a primary cell culture is a culture of cells, tissues or organs taken directly from organisms before the first subculture. Cells are expanded in culture when they are placed in a growth medium under conditions that facilitate growth and/or division, resulting in a larger population of cells.
• a cell line is a population of cells formed by one or more subcultivations of a primary cell culture. Each round of subculturing is referred to as a passage. It will be understood by those skilled in the art that there may be many population doublings during the period of passaging.
• Culture media suitable for use in the process of the present invention are known in the art, including, basal media supplemented with serum, serum-free media, protein-free media or chemically defined growth media.
• a 'conditioned' medium may be used.
• a conditioned medium is one in which a specific cell or population of cells has been cultured and then removed. While these cells are cultured in the medium they secrete cellular factors that can provide support to other cells.
• the medium containing the cellular factors is the 'conditioned' medium.
• the cells used for conditioning the medium can be of the same type as subsequently cultivated, a different cell type or a combination of both.
• Hair follicle mesenchymal cells were isolated essentially as described in EP980270 with the modifications described below.
• Human skin tissue samples were washed 3 times with Minimal Essential Medium (MEM, Sigma) containing 1 ⁇ g/ml amphotericin and 10 ⁇ g /ml gentamycin.
• MEM Minimal Essential Medium
• anagen 'end bulbs' were dissected using fine surgical scissors and placed into small volumes (typically 100-200 ⁇ l) of MEM.
• the end bulbs were inverted using needles, and the papilla dissected and the sheath extracted.
• the papillae and sheaths were then transferred separately to 4 well cell culture plates (Nunc).
• Ten papillae and 10 sheath were transferred per well in 1ml of MEM supplemented with 20% foetal bovine serum, 0.5 ⁇ g/ml amphotericin and 5 ⁇ g /ml gentamycin.
• the four well cell culture plates were incubated under sterile and standard conditions (37°C, 5% carbon dioxide). After 10 days cell growth, cells were detached from each well (using standard methods well established in the art) and transferred separately to a 35mm diameter cell culture dish (Nunc).
• 'AVDS' dermal sheath
• 'AVDP' dermal papilla
• AVDS and AVDP cell lines were established form a number of different human tissue samples. A summary of these cell lines which are described in the following examples is provided in Table 1 below.
• Dermal fibroblast (hereinafter referred to as 'AVDF') cell lines were established from the same human skin tissue samples described above. The dermis was separated from the adipose layer and then dissected under a microscope into pieces of approximately 2- 3mm 2 surface area. Dissected tissue was transferred to a T25 cell culture flask (Nunc) containing MEM supplemented as described for the dermal sheath and dermal papilla cell lines. The T25 cell culture flasks containing dermal fibroblast (AVDF) cell lines were incubated under sterile and standard conditions (as described previously). The dermal fibroblast (AVDF) cell lines were then further expanded using the same conditions when the cultures had reached confluency. AVDF cell lines were established form a number of different human tissue samples. A summary of these cell lines which are described in the following examples is provided in Table 1 below.
• the MEM growth medium supplemented with 10% FBS was replaced in each flask on day 3.
• the cells were harvested after 5 days using standard cell detachment methods well established in the art and the number of AVDS viable cells determined using trypan blue staining and cell counts as is well established in the art. Surprisingly, no viable AVDS cells were recovered from the culture with either Cytodex I or Cytodex III microcarriers.
• Cytodex I and Cytodex III microcarriers are widely-used successfully in the industry for the culture of a wide range of mammalian cell types. Both Cytodex I and Cytodex III are composed of Dextran. Cytodex I is composed of a dextran matrix with cationic DEAE groups whilst Cytodex III is composed of dextran matrix coated with denatured porcine collagen.
• AVDS dermal sheath cells
• 2D MicroHex microcarriers were used to culture cell lines AVDS1, AVDS2 and AVDP1.
• the microcarriers were prepared and sterilised using standard methods recommended by the manufacturer.
• the 2D MicroHex microcarriers (Nunc) are composed of the same material/surface chemistry that is used for static cell culture (Example 1 and 2).
• Microcarrier concentrations of 1.0 cm 2 /ml, 2.0 cm 2 /ml and 4.4cm 2 /ml were used and 4 x 10 5 AVDS1 , AVDS2, AVDS3 and AVDP1 cells per 250ml cell culture shake flask (Corning) were seeded in a 40ml total volume MEM (Sigma M4655) supplemented with 10% fetal bovine serum (FBS).
• the cell culture flasks were placed in an orbital shaker at 37°C with agitation at 70rpm.
• the cell culture flasks were harvested after 3 to 10 days and the total viable cell concentration determined using methods well established in the art. The results obtained are presented in Table 2.
• the poor growth achieved by mesenchymal dermal stem cell lines AVDS1 , AVDS2 and AVDP1 were not expected and totally surprising given that in static culture these cell lines adhere to the surface of the flask and proliferate readily.
• the 2D MicroHex microcarriers are composed of the same material/surface chemistry as that used in the static cell culture flasks.
• Table 2 Growth of cell lines AVDS1, AVDS2, AVDS3 and AVDP1 on MicroHex Microcarriers
• microcarriers prepared were: FACT and Pronectin F (Sigma, SoIoHiII) CGEN 102-L, HLX11-170, Pro-F 102-L, FACT 102-L, P102- L and P plus 102-L (Thermo Fisher). FACT and Pronectin F were used at 5g/L, CGEN 102-L, HLX11-170, Pro-F 102-L, FACT 102-L, P102-L and P plus 102-L were used at 2.5g/L.
• FACT and Pronectin F Sigma, SoIoHiII
• the HLX11-170 microcarriers are composed of polystyrene coated with cationic trimethyl ammonium.
• the other microcarriers which surprisingly did not support growth were composed of either a type I porcine collagen with a cationic charge, uncoated polystyrene plastic or plastic with either a surface charge or coated with recombinant fibronectin or fibronectin.
• AVDS2 cells The poor growth of AVDS2 cells was especially surprising given that the prior art demonstrates that a wide range of cells such as embryonic stem cell lines, bone marrow derived mesenchymal stem cell lines and lung fibroblast cell lines will readily grow on microcarriers composed of collagen.
• the bioreactor was cultured at a temperature of 36.5°C, pH 7.0 (manual control by carbon dioxide gas sparging and/or addition of sodium hydroxide) and an impeller speed of 80 rpm. After 2 days incubation under the conditions described a further 1.0L MEM cell culture growth medium supplemented with 10% FBS and 0.2% pluronic F- 68 was added aseptically to the cell culture bioreactor. The incubation was continued under the conditions described for a further five days. The bioreactor was then harvested and 4.4 x 10 8 AVDS4 viable cells were recovered from the bioreactor. The results demonstrate that HLX11-170 microcarriers can be used for the reproducible generation of large numbers of stem cells with well-defined characteristics under tightly controlled and scaleable conditions.
• the AVDS4 cells recovered from the HLX11-170 microcarrier bioreactor culture described in Example 4 were used to prepare frozen cell stocks using methods well established in the art. After storage in liquid nitrogen for 4 days, 2 ampoules were thawed and diluted in 30ml MEM cell culture growth medium (Sigma M4655) supplemented with 10% fetal bovine serum (FBS). This AVDS4 cell suspension was then used to seed 6 cell culture flasks as shown in Table 4. The shake flasks with HLX11-170 microcarriers were placed in an orbital shaking incubator at 37°C and 70 rpm and the 225cm 2 cell culture flasks were grown under static culture conditions (37°C, 5% carbon dioxide) with no microcarriers. Flasks were harvested after 5 to 6 days and the viable cell concentration determined as described previously. Table 4: Growth of AVDS4 cells in static and shake flask culture following harvest from HLX11-170 microcarriers
• the flasks was harvested and the viable cell concentration determined as described previously. No viable cells were recovered.
• the HLX11-170 microcarriers (Flasks 4, 5 and 6) did not support growth of the AVSD4 cells (which had previously been harvested from a HLX11-170 microcarrier bioreactor culture - Example 5) and a lower number of cells were recovered from the flasks than originally seeded.
• the AVDS4 cells harvested from a HLX11-170 microcarrier bioreactor culture (Example 5) did grow and proliferate in static culture in the absence of microcarriers (Flasks 1, 2, 3).
• Example 6 One 225cm 2 cell culture flask (Nunc) and two 75cm 2 cell culture flasks (Nunc) of dermal sheath cells AVDS5 grown in static culture were detached using methods well established in the art. 2.2 x 10 6 and 1.1 x 10 6 AVDS5 cells were then used to seed a 1 L cell culture shake flask (Nunc) and a 500ml cell culture shake flask (Nunc) respectively.
• the cell culture flasks contained 1g/L CultiSpher S microcarriers (prepared as described previously) in a total volume of 200ml and 100ml of MEM cell culture growth medium supplemented with 10% FBS in the 1 L and the 500ml shake flasks respectively.
• the headspace was equilibrated with 5% CO 2 in air gas and the flasks were transferred to an orbital shaker incubator at 37°C, 40rpm. After 7 days incubation under the conditions described, the contents of the flasks were harvested and cells were detached from the microcarriers. 9.1 x 10 6 AVDS5 cells were used to inoculate a 2L glass cell culture bioreactor (Applikon) in a total volume of 500ml of MEM cell culture growth medium supplemented with 10% FBS + 0.2% pluronic F-68 with 2g/L CultiSpher S microcarriers. The bioreactor was cultured at 36.5°C, pH 7.0 (maintained as described in Example 6) and an agitator speed of 50rpm.
• Example 7 Three 225cm 2 cell culture flasks (Nunc) of dermal papilla cells AVDP1 grown in static culture were detached. 2.0 x 10 6 AVDP1 cells were used to seed each of two 1.5L cell culture spinner flasks containing 1.5g/L CultiSpher S microcarriers in a total volume of 250ml of MEM cell culture growth medium supplemented with 10% FBS. The headspace was equilibrated with 5% CO 2 in air gas. The spinner flask was transferred to a cell culture incubator (37°C) and agitated at 30rpm using a magnetic stirrer base.
• 125ml spent cell culture growth medium was removed from each flask and replaced with fresh cell culture growth medium as described above.
• the AVDP1 cells were detached from the microcarriers.
• 1.6 x 10 7 cells were used to inoculate a 2L glass cell culture bioreactor (Applikon) in a total volume of 2L of MEM cell culture growth medium supplemented with 10% FBS, 0.2% pluronic F-68 and 1.5g/L CultiSpher S.
• the bioreactor was cultured at 37°C, pH 7.10 (controlled as described in Example 6), dissolved oxygen tension 20% (air saturation) and an agitator speed of 50rpm.
• the dissolved oxygen level was maintained using CO 2 and N 2 sparging. After 4 and 8 days incubation under the conditions described, 1 L of spent media was removed from the bioreactor and replaced with 1L of fresh medium (as described above). After 4 days incubation, the agitator speed was increased to 70rpm, and at 11 days incubation, the agitator speed was increased to 90rpm. Samples were removed from the bioreactor periodically and the cells were detached and the number of viable cells determined as described previously. The viability and cell number are presented in Figure 1. A significantly high peak viable cell density of 2.9 x 10 9 AVDPI cells was achieved.
• Example 8 CultiSpher S microcarriers were prepared as described previously. The microcarriers were then used to culture dermal fibroblast cell line AVDF1. A 250ml shake flask (Corning) with 25ml MEM cell culture growth medium (Sigma M4655) supplemented with 10% fetal bovine serum (FBS) was seeded with 5 x 10 5 AVDF1 cells and 1g/L CultiSpher S microcarriers. The flask was placed in an orbital shaker incubator at 37 0 C and 70rpm.
• MEM cell culture growth medium Sigma M4655
• FBS fetal bovine serum
• Two 225cm 2 cell culture flasks (Nunc) of dermal fibroblast cells AVDF2 grown in static culture were detached and counted using methods well described in the art.
• 2.0 x 10 6 AVDF2 cells were used to seed each of two 1L cell culture shake flasks (Nunc) containing a total volume of 90ml MEM cell culture growth medium supplemented with 10% FBS containing 2g/L Cultispher S microcarriers (prepared as described previously).
• the headspace of the cell culture flasks was equilibrated with 5% CO 2 in air gas.
• the cell culture flasks were transferred to an orbital shaker incubator at a temperature of 37°C and agitation at 40 rpm.
• the flasks were incubated under the conditions described for 24h and a further 90ml of cell culture growth medium (as described above) added to each flask.
• the flasks were returned to the shaker incubator under the conditions described above and the incubation continued for 7 days.
• the contents of the flasks were harvested and cells were detached (as described previously) from the microcarriers.
• 1.65 x 10 7 AVDF2 cells were then used to inoculate a 3L spinner cell culture flask (Corning) in a total volume of 750ml MEM cell culture growth medium supplemented with 10% FBS containing 2g/L CultiSpher S microcarriers.
• the headspace was equilibrated with 5% CO 2 in air gas.
• the spinner flask was transferred to a cell culture incubator (37°C) and agitated at 30rpm using a magnetic stirrer base. After 2 days incubation under the conditions described, a further 750ml of the cell culture growth medium described above was added to the spinner flask. The incubation was continued under the conditions described above for a further 4 days. The contents of the cell culture spinner flask were harvested and cells recovered. 4.0 x 10 7 AVDF2 cells were recovered at a viability of 91%.
• AVDF2 dermal fibroblast cells AVDF2 grown in static culture was detached and counted using methods well described in the art.
• 2.2 x 10 6 AVDF2 cells were used to seed a 1 L spinner cell culture flask (Wheaton) in a total volume of 240ml MEM cell culture growth medium supplemented with 10% FBS containing 1.5g/L CultiSpher S microcarriers (prepared as previously described).
• the headspace of the flask was equilibrated with 5% CO 2 and 2% O 2 in nitrogen gas.
• the spinner flask was transferred to a cell culture incubator (37°C) and agitated at 40rpm using a magnetic stirrer base.
• the recovered cells (2.4 x 10 6 AVDF2 cells) were then used to seed each of two 1 L spinner cell culture flasks (Wheaton) in a total volume of 300ml cell culture growth medium (described above) per flask containing 1.5g/L CultiSpher S microcarriers (prepared as previously described).
• the headspace of the flask was equilibrated with 5% CO 2 and 2% O 2 in nitrogen gas.
• the spinner flask was transferred to a cell culture incubator (37°C) and agitated at 40rpm using a magnetic stirrer base.
• the bioreactor was cultured at 37 C C, pH 7.0 (controlled as described in Example 7), dissolved oxygen tension 2.0% (air saturation) and an agitator speed of 50rpm which was increased gradually to 90rpm over the course of the culture.
• the dissolved oxygen level in the cell culture was maintained using CO 2 and N 2 gas sparging.
• Emulsion C antifoam agent Sigma was added to the bioreactor when foaming occurred.
• 20% of the total culture volume in the bioreactor was replaced with fresh cell culture growth medium every 24 hours. Samples were removed from the bioreactor periodically and the cells were detached and the number of viable cells determined as described previously. The viability and cell number are presented in Figure 2. A viable cell number of 5.0 x 10 8 cells was reached after 18 days in culture.
• a vial of cell line AVDS6 was thawed and 50ml of MEM cell culture growth medium supplemented with 10% FBS was added. 4.2 x 10 6 cells at a viability of 90% were recovered. 2 x 10 6 AVDS6 cells were used to seed each of two spinner cell culture flasks (Wheaton) with 8.0 x 10 3 cells/mL and 1.5 g/L CultiSpher S microcarriers (prepared as described previously) in a total volume of 250 ml. growth medium (described above). The flasks were incubated as described in Example 10.
• a volume of 30ml of the cell culture supernatant was removed from each spinner flask and replaced with 30ml fresh cell culture growth medium (described above). After a total of 8 days incubation, the contents of the flasks were harvested and 4.9 x 10 7 cells at a viability of 80% were recovered.
• 1.6 x 10 7 AVDS6 cells were then used to inoculate a 2L glass cell culture bioreactor (Applikon) in a total volume of 1.5L of MEM cell culture growth medium supplemented with 10% FBS, 0.2% pluronic F- 68 and 1.5g/L CultiSpher S microcarriers (prepared as described previously).
• the bioreactor was cultured at 37°C, pH 7.0 (controlled as described in Example 7), dissolved oxygen tension 2.0% (air saturation) and an agitator speed of 50rpm which was increased gradually to 90rpm over the course of the culture.
• the dissolved oxygen level was maintained as described in Example 10.
• Emulsion C antifoam agent Sigma was added to the bioreactor when foaming occurred.
• a further 500ml of growth medium (described above) was added to the bioreactor. From 5 days in culture, 5% of the total culture volume was replaced every 24h. Samples were removed from the bioreactor periodically and the cells were detached and the number of viable cells determined (as described previously). The viability and cell number are presented in Figure 3.
• a viable cell number of 9.0 x 10 7 cells was observed in a 2L bioreactor after 17 days in culture.
• Example 12 The cells from five 225cm 2 flasks (Nunc) of cell line AVDS6 grown in static culture were detached and counted using methods well described in the art. 2.0 x 10 6 AVDS6 cells were used to seed each of two 1 L spinner cell culture flasks (Wheaton) in a total volume of 200ml MEM cell culture growth medium supplemented with 10% FBS containing 1.5g/L CultiSpher S microcarriers (prepared as described previously). The headspace was equilibrated with 5% CO 2 and 2% O 2 in nitrogen gas. The spinner flask was transferred to a cell culture incubator (37°C) and agitated at 40rpm using a magnetic stirrer base.
• a cell culture incubator 37°C
• the bioreactor was cultured at a temperature of 36.5°C, pH at 7.1 (controlled as described in Example 7), dissolved oxygen tension 5.0% (air saturation) and an agitator speed of 70rpm (which was increased gradually to 110rpm over the course of the culture).
• the dissolved oxygen level was maintained as described in Example 10.
• Emulsion C antifoam agent Sigma was added to the bioreactor when foaming occurred. From 4 to 7 days incubation under the conditions described, 10% of the total culture volume was replaced every 24 hours. From 8 to 10 days in culture, 15% of the total culture medium and from 11 to 22 days in culture, 20% of the total culture volume was replaced every 24 hours. Samples were removed from the bioreactor periodically and the cells were detached and the number of viable cells determined (as described previously). The viability and cell number are presented in Figure 4. A maximum viable cell number of 4.1 x 10 8 cells was observed in a 5L bioreactor after 23 days in culture, demonstrating successful process scale up to 5L bioreactor scale.
• Example 13 Three 225cm 2 flasks (Nunc) of cell line AVDS6 grown in static culture were detached and counted using methods well described in the art. 4.3 x 10 6 cells at a viability of 84% were recovered. 3.0 x 10 6 AVDS6 cells were then used to seed a 1 L spinner cell culture flask (Wheaton) in a total volume of 300ml low serum (2%) growth medium supplemented with 4mM glutamine (Sigma) containing 1.5g/L CultiSpher S microcarriers (prepared as described previously). The culture was incubated as described in Example 12.
• the bioreactor was cultured at a temperature of 36.5°C and pH 7.1 (controlled as described in Example 7), dissolved oxygen tension 5.0% (air saturation) and an agitator speed of 70rpm which was increased gradually to 130rpm over the course of the culture.
• the dissolved oxygen level was maintained as described in Example 10.
• Emulsion C antifoam agent Sigma was added to the bioreactor when foaming occurred. From 4 to 7 days incubation under the conditions described, 10% of the total culture volume was replaced with fresh growth medium every 24h. From 8 to 10 days in culture, 15% of the total culture volume and from 11 to 20 days in culture, 20% of the total culture volume was replaced with fresh growth medium every 24h.
• a vial of cell line AVDF3 was thawed and re-suspended using 45ml of MEM cell culture growth medium supplemented with 10% FBS. 7.7 x 10 6 AVDF3 cells at a viability of 94% were recovered. 2.4 x 10 6 cells were used to seed each of two 1 L spinner cell culture flasks (Wheaton) with 8.0 x 10 3 cells/mL and 1.5 g/L CultiSpher S microcarriers (prepared as described previously) in a total volume of 300 ml. growth medium (described above). The flasks were incubated as described in Example 10.
• 1.6 x 10 7 cells were used to inoculate a glass cell culture bioreactor (Applikon) in a total volume of 2L of MEM cell culture growth medium supplemented with 10% FBS, 0.2% pluronic F-68 and 1.5g/L CultiSpher S microcarriers (prepared as described previously).
• the bioreactor was cultured at a temperature of 36.5°C, pH 7.0 (controlled as described in Example 7), dissolved oxygen tension 5.0% (air saturation) and an agitator speed of 70rpm which was increased gradually to 120rpm over the course of the culture. The dissolved oxygen level was maintained as described in Example 10.
• Emulsion C antifoam agent (Sigma) was added to the bioreactor when foaming occurred. From 4 to 7 days under the conditions described, 10% of the total culture volume was replaced with fresh growth medium every 24h. From 8 to 10 days in culture, 15% of the total culture volume and from 11 to 20 days in culture, 20% of the total culture volume was replaced with fresh growth medium every 24 hours. Samples were removed from the bioreactor periodically and the cells were detached and the number of viable cells determined as described previously. The viability and cell number are presented in Figure 6. A maximum viable cell number of 3.3 x 10 8 cells was achieved on day 15.
• One 225cm2 cell culture flask (Nunc) of dermal fibroblast cells AVDF3 grown in static culture conditions were detached and counted using methods well described in the art.
• 2.3 x 10 6 cells were used to seed a 1.5L cell culture spinner flask containing 1.5g/L CultiSpher S microcarriers (prepared as described previously) in a total volume of 330ml of serum free growth medium supplemented with 2mM glutamine (Sigma).
• the headspace of the flask was equilibrated with 5% CO 2 , 2% O 2 gas.
• the spinner flask was transferred to a cell culture incubator at 37°C and agitated at 35rpm using a magnetic stirrer base.
• the bioreactor was cultured at a temperature of 36.5°C, pH 7.0 (controlled as described in Example 7), dissolved oxygen tension 5.0% (air saturation) and an agitator speed of 40rpm which was increased gradually to 60rpm over the course of the culture.
• the dissolved oxygen tension was maintained as described in Example 10.
• Emulsion C antifoam agent Sigma was added to the bioreactor when foaming occurred. After 4 days incubation under the conditions described, 200ml of culture supernatant was removed from the bioreactor and replaced with fresh serum free growth medium (as described above). A further 200ml of culture supernatant was removed and replaced with fresh serum free growth medium (as described above) on days 6, 8, 10,11 ,13, 15, 17 and 21.
• the cells from two 225cm 2 flasks (Nunc) of cell line AVDS2 grown in static culture conditions were detached and counted using methods well described in the art.
• 3.1 x 10 6 cells at a viability of 90% were recovered.
• 2.9 x 10 6 AVDS2 cells were used to seed each of two 1 L spinner cell culture flasks (Wheaton) in a total volume of 300ml MEM cell culture growth medium supplemented with 10% FBS containing 1.5g/L CultiSpher S microcarriers (prepared as described previously).
• the headspace of the flask was equilibrated with 5% CO 2 and 2% O 2 in nitrogen gas.
• the spinner flask was transferred to a cell culture incubator (37°C) and agitated at 40rpm using a magnetic stirrer base. After 4 days incubation under the conditions described, and on each day up to and including 7 days incubation, a volume of 30ml of the cell culture supernatant was removed from each spinner flask and replaced with 30ml fresh cell culture growth medium (described above). After 8 days incubation under the conditions described, the flasks were harvested and 4.7 x 10 7 cells at a viability of 96% were recovered.
• AVDS2 cells 2.0 x 10 7 AVDS2 cells were used to inoculate a glass cell culture bioreactor (Applikon) in a total volume of 2L of MEM cell culture growth medium supplemented with 10% FBS, 0.2% pluronic F-68 and 1.5g/L CultiSpher S (prepared as described previously).
• the bioreactor was cultured at a temperature of 36.5°C, pH 7.1 (controlled as described in Example 7), dissolved oxygen tension 5.0% (air saturation) and an agitator speed of 40rpm which was increased gradually to 70rpm over the course of the culture.
• the dissolved oxygen level was maintained as described in Example 10.
• Emulsion C antifoam agent Sigma was added to the bioreactor when foaming occurred.
• the impeller (agitation) configuration used in this example was altered to that in Examples 7 to 15.
• the impeller configuration used in this example consisted of 2 impellers with a ratio of impeller diameter to bioreactor diameter of 0.4 and 0.3.
• an impeller of a ratio of 0.3 was used at the base of the impeller shaft, i.e. located at the base of the cell culture vessel.
• the impeller with a 0.4 diameter ratio was used at the base of the impeller shaft/base of the cell culture vessel, and the impeller with a 0.3 diameter ratio was attached to the impeller shaft in a central position (relative to the impeller at the base of the shaft and the final cell culture medium volume (at harvest).
• Samples were removed from the bioreactor periodically and the cells were detached and the number of viable cells determined as described previously. The viability and cell number are presented in Figure 7. A maximum viable cell number of 2.0 x 108 cells was observed after 10 days in culture.
• the altered impeller configuration lead to a significant improvement to the process.
• a lower agitation speed could be used during the cell culture - reducing the potential negative effects of shear stress on the cells without influencing the optimum mixing required to maintain good cell growth and viability.
• the cell culture time taken to reach a high viable cell number was significantly reduced.
• Example 13 it can be seen that a peak cell number for a DS cell line was reached on day 23. In the present example, a similar peak cell number was reached on day 11 reducing cell culture time significantly. It will be evident to those skilled in the art how a reduction in cell manufacturing time will reduce manufacturing time and costs when the process is scaled-up to manufacture cells commercially for therapeutic applications.
• Example 17 Skin-derived precursors are a self-renewing, multipotent precursor population of cells which can be isolated from dermal cells.
• SKPs cells have been well described in the art as exhibiting a similar gene expression profile to embryonic neural crest stem cells and thus can be used to generate neural crest derivatives, including Schwann cells and neurons of the peripheral nervous system.
• SKPs cells can be isolated by culturing dermal cells using high concentrations of fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF) in the growth medium.
• FGF2 fibroblast growth factor 2
• EGF epidermal growth factor
• the SKPs proliferate as floating spheres and thus are easily visualised and quantified using methods well established in the art.
• the cells were then centrifuged at 180 x g for 5 minutes and resuspended in SKPs growth medium consisting of 74% DMEM (Sigma), 24% F12 with Glutamax (Invitrogen), 0.1% Penicillin/Streptomycin (Sigma), 40 ng/mL human FGF-2 (R&D Systems) 20 ng/mL human EGF (R&D Systems) and 2% B27 supplement (Invitrogen).
• 2.5 x 10 5 cells were then transferred to a 25cm 2 cell culture flask (Nunc) and incubated as described above. Every 3 to 4 days, 1ml of cell-free supernatant was removed from the flask and replaced with 1 ml of SKPs growth medium containing a 5 times greater concentration of EGF, FGF-2 and B27 supplement.
• the cells were then centrifuged at 180 x g for 5 minutes and resuspended in SKPs growth medium as described above. 2.5 x 10 5 cells were then transferred to a 25cm 2 cell culture flask (Nunc) and incubated as described above. Every 3 to 4 days, 1 ml of cell supernatant was removed from the flask and replaced with 1 ml of SKPs growth medium containing a 5 times greater concentration of EGF, FGF-2 and B27 supplement.
• the headspace of the flask was equilibrated with 5% CO 2 and 2% O 2 in nitrogen gas.
• the spinner flask was transferred to a cell culture incubator (37°C) and agitated at 40rpm using a magnetic stirrer base. After 4 days incubation under the conditions described, and on each day up to and including 6 days incubation, a volume of 25ml of the cell culture supernatant was removed from each spinner flask and replaced with 25ml fresh cell culture growth medium (described above). After 7 days incubation under the conditions described, the flask was harvested and 2.1 x 10 7 cells at a viability of 95% were recovered.
• 1.3 x 10 5 cells were then seeded into a 125ml cell culture shake flask (Corning) containing a 12.5 cm 2 piece of a 3-dimensional biocompatible tissue regeneration scaffold (Integra Dermal Regeneration template (Integra Life Sciences)) in a total volume of 17ml cell culture growth medium (described above).
• the Integra Dermal Regeneration template was positioned in the flask so that the silicon layer faced the cell culture flask base and the collagen surface was faced upwards and thus bathed in cell suspension/cell growth medium.
• the headspace of the flask was equilibrated with 5% CO 2 and 2% O 2 in nitrogen gas and transferred to an orbital shaking incubator set to 37°C and 60rpm.
• the data indicate infiltration and proliferation of the cells into the Integra Dermal Regeneration template. These data further exemplify the utility of the process demonstrating that cells manufactured using CultiSpher S microcarrier culture retain the ability to infiltrate and proliferate within a biocompatible 3-dimensional scaffold such as Integra Dermal Regeneration template. It will be evident to those skilled in the art of dermal regeneration that if the cells are present only on the outer surface of a scaffold then critical aspects of wound healing such as, but not limited to, extracellular matrix production and vascularisation will be limited and the likelihood of successful tissue regeneration would below/poor.
• Example 19 It is well established in the art that apoptosis is an important and active regulatory pathway of cell growth and proliferation. Apoptosis may be effected by (but limited to) growth media, stress conditions or other parameters. Determination of the level of apoptotic cells in a cell culture population can be used as a tool to monitor and assess the negative effects of process conditions, cell expansion, etc.
• the annexin V assay is well established in the art and was used to measure apoptosis in cells grown on CultiSpher S microcarriers in a bioreactor with MEM + 10% FBS cell culture growth medium.
• Control culture was produced by expanding AVDS4 from a cell bank seeded at the same level as the flask described previously into a 225cm 2 flask in MEM + 10% FBS.
• This control culture had not been processed using microcarriers or bioreactor culture. Both flasks were incubated for 5 days at 37 0 C, 5% CO 2 and then each cell culture was prepared for analysis as follows. The growth medium was separated from the cell monolayer. The cell monolayer was washed and then detached from the tissue culture flask as is well established in the art. The growth medium, wash and detached monolayer were then re-combined. The cells were centrifuged at 300 x g for 5 minutes at room temperature. The supernatant was aspirated and the cell pellet was resuspended in fresh MEM + 10% FBS at 5 x 10 5 cells/ml.
• cells have been cultured in a bioreactor using CultiSpher microcarriers and that were in culture for a significantly longer period than the control cells, show comparable and acceptable cell viability/ level of apoptotic cells.
• TGF- ⁇ 1 Transforming growth factor beta 1
• TGF- ⁇ 1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions and has been identified as having a key role in wound healing.
• a TGF- ⁇ 1 duo ELISA assay (R&D Systems) was used to determine levels of the protein in culture supernatant from cells grown on CultiSpher S microcarriers in a bioreactor with MEM + 10% FBS cell culture growth medium. Cells from continued passage in static culture using tissue culture flasks, also grown in MEM + 10% FBS, were used as a control to compare to the levels of TGF- ⁇ 1 produced by the two cell populations.
• Control culture was produced by expanding AVDS4 from a cell bank seeded at the same level as the flask described previously into a 225cm 2 flask in MEM + 10% FBS. This control culture had not been processed using microcarriers or bioreactor culture. Both flasks were incubated for 5 days at 37°C, 5% CO 2 and the residual cell culture medium was then harvested and assayed to determine the concentration of TGF- ⁇ 1 using a ELISA assay (R&D Systems). Samples were analysed in duplicate and the results are shown in Figure 10. The TGF- ⁇ 1 levels were normalised to cell concentration vs. flask surface area.

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