CN102145128A - Medicinal composition for preventing and treating cardiac and cerebral vascular diseases and preparation method thereof - Google Patents
Medicinal composition for preventing and treating cardiac and cerebral vascular diseases and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a medicinal composition for preventing and treating cardiac and cerebral vascular diseases, consisting of antler blood, ginseng, milkvetch root, Chinese angelica, Chinese caterpillar fungus, medlar, fleeceflower root, manyprickle acathopanax root, fiveleaf gynostemma herb, red sage root, safflower, lucid ganoderma, red paeony root, Szechuan lovage rhizome, earthworm, pueraria root, amber, hawthorn, algae, rose flower, tall gastrodia tuber and bear gall. The medicinal composition has the functions of adjusting the immunity of the organism and the functions of a neuroendocrine system, improving the circulation of cerebral blood substances, reducing the blood fat and blood viscosity, improving the brain function, preventing and treating the cardiac and cerebral vascular diseases, delaying the ageing and the like. The medicinal composition can prepared into capsules, tablets, granules, pills, soft capsules and other dosage forms.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition and preparation method thereof, this pharmaceutical composition can be regulated immunity of organisms specifically, regulates the neuroendocrine system function; Improve brain blood material circulation, blood fat reducing, blood glue, and improve brain function, pharmaceutical composition of control cerebrovascular and slow down aging and preparation method thereof.
Background technology
Cardiovascular and cerebrovascular disease is a commonly encountered diseases clinically, China particularly the northern area of China belong to regular incidence, frequently-occurring disease, and ascendant trend is gradually arranged in recent years, constituted huge prestige association to human health.Although along with the fast development of scientific and technological level, this sick treatment level is improved a lot, new drug also emerges in an endless stream, but still exists many insoluble contradictions.As the control to concurrent arrhythmia, acute heart failure, the reparation of perfusion back myocardial damage is because of for a long time, the avoiding etc. of the toxic and side effects that produces of continuing to take medicine.Therefore, it is rapidly lasting that development has effect, and stable curative effect is reliable and do not have the medicine with cardiovascular and cerebrovascular disease of serious toxicity, just becomes current main target and direction.
The SHANHAIDAN JIAONANG that the Sanitation Ministry medicine standard WS3-253 (Z-54)-93 (Z) records is by the capsule of flavour of a drug through being processed into such as Radix Notoginseng, Radix Ginseng, the Radix Astragali, Flos Carthami, goat blood powder, Semen Cassiae, Radix Puerariae, Fructus Citri Sarcodactylis, Sargassum, Radix Polygoni Multiflori, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiongs.This medical instrument has promoting blood circulation to remove obstruction in the collateral, treatment heart arteries and veins stasis of blood resistance Xiong Paralysis effect.Pharmacological research confirms that this product has benefiting QI and nourishing blood, regulates the central nervous system, hormonal system and raise immunity, and the energy coronary artery dilating increases cerebral arteries and crown blood flow volume, improves myocardial oxygen delivery, reduces myocardial oxygen consumption.And can obviously bring high blood pressure down, arrhythmia reduces myocardial oxygen consumption, reduces cholesterolemia, triglyceride, high density lipoprotein increasing, and anticoagulant suppresses effects such as thrombosis.
A kind of pharmaceutical composition of preventing and treating cardiovascular and cerebrovascular disease of the present invention is on the SHANHAIDAN JIAONANG basis, its prescription has been carried out the dialectical adjustment of science by the Chinese medical discrimination theory, its effect particularly is better than SHANHAIDAN JIAONANG at aspects such as strengthening body immunity, reduction cholesterolemia, triglyceride, prevention and treatment thrombosis, it is a kind of bonded medicine of preventing, control, show that through clinical research it is more lasting to act on, toxic and side effects is little, the patient can take for a long time, has good market prospect.
Summary of the invention
Purpose of the present invention is exactly that patient to cardiovascular and cerebrovascular disease provides that a kind of high-drug-effect, effect are lasting, bonded pharmaceutical composition of control and preparation method thereof, and pharmaceutical composition of the present invention can be regulated immunity of organisms, regulates neuroendocrine system; Improve the brain blood circulation, blood fat reducing, blood glue, and improve brain function, reach the purpose of control cerebrovascular disease and slow down aging.This pharmaceutical composition can be made dosage forms such as capsule, tablet, granule, drop pill, soft capsule at needs of patients in addition.
Technical scheme of the present invention is as follows:
A kind of pharmaceutical composition of preventing and treating cardiovascular and cerebrovascular disease is characterized in that it being to be formed by the following raw medicaments in portion by weight processing and preparing:
Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 3-5g Radix Ginseng 1-3g Radix Astragali 40-45g Radix Angelicae Sinensis 9-12g Cordyceps 1-3g Fructus Lycii 10-12g Radix Polygoni Multiflori (system) 23-26g Radix Et Caulis Acanthopanacis Senticosi 10-12g Herb Gynostemmae Pentaphylli 10-12g Radix Salviae Miltiorrhizae 20-30g Flos Carthami 10-13g Ganoderma 1-3g Radix Paeoniae Rubra 10-12g Rhizoma Chuanxiong 23-27g Pheretima 10-12g Radix Puerariae 23-27g succinum 1-3g Fructus Crataegi 10-12g Sargassum 10-12g Flos Rosae Rugosae 2-5g Rhizoma Gastrodiae 5-7g Fel Ursi 1-3g
A kind of pharmaceutical composition of preventing and treating cardiovascular and cerebrovascular disease is characterized in that becoming the optimum scheme by following bulk drugs processing and preparing:
Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 4g Radix Ginseng 2g Radix Astragali 43g Radix Angelicae Sinensis 11g Cordyceps 2g Fructus Lycii 11g Radix Polygoni Multiflori (system) 25g Radix Et Caulis Acanthopanacis Senticosi 11g Herb Gynostemmae Pentaphylli 11g Radix Salviae Miltiorrhizae 25g Flos Carthami 11g Ganoderma 2g Radix Paeoniae Rubra 11g Rhizoma Chuanxiong 25g Pheretima 11g Radix Puerariae 25g succinum 2g Fructus Crataegi 11g Sargassum 11g Flos Rosae Rugosae 4g Rhizoma Gastrodiae 6g Fel Ursi 2g
A kind of pharmaceutical composition of preventing and treating cardiovascular and cerebrovascular disease of the present invention can be prepared into dosage forms such as capsule, tablet, granule, drop pill, soft capsule, and wherein capsule preparation method thereof comprises the step of following order:
(1) it is standby to take by weighing the above-mentioned raw materials medicine in proportion;
(2) getting the Radix Astragali, Radix Puerariae, Flos Carthami, Herb Gynostemmae Pentaphylli, Rhizoma Chuanxiong, Flos Rosae Rugosae, Sargassum, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Pheretima, Fructus Crataegi, Rhizoma Gastrodiae, Radix Polygoni Multiflori ten Six-element medical materials gives as one thinks fit cataclasm;
(3) above-mentioned cataclasm medical material is put in the extraction pot, added 4 times of amount dippings of 75-85% ethanol 12 hours, the heating and refluxing extraction secondary, each 2 hours, merge extractive liquid, was put cold, filter, decompression recycling ethanol to relative density is the clear paste of 1.10-1.20 (60 ℃), and is standby;
(4) medicinal residues that above-mentioned ethanol extraction is crossed add water 8-10 and doubly measure, the heating and refluxing extraction secondary, and each 2 hours, merge extractive liquid, filters, and is concentrated into relative density and is the clear paste of 1.10-1.20 (60 ℃), and was standby;
(5) with above-mentioned (3) and (4) clear paste of extracting mix, dry in vacuum desiccator, get dry extract;
(6) give as one thinks fit cataclasm and dried cream to mix pulverizing all the other Six-element medical materials such as Sanguis Cornu Cervi Pantotrichum, cross 100 mesh sieves, make granule, incapsulate, promptly.
The medicated powder of crossing 100 mesh sieves in above-mentioned (6) can be made tablet, granule, drop pill, soft capsule dosage form by the preparation method preparation of tablet, granule, drop pill, soft capsule dosage form.
A kind of its function of pharmaceutical composition of preventing and treating cardiovascular and cerebrovascular disease of the present invention has the adjusting neuroendocrine system; Improve brain blood material circulation, blood fat reducing, blood glue, and improve brain function, control cerebrovascular and function in delaying senility.
Why the present invention adopts above-mentioned medical material is raw material, is because they have following effect:
Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) warming YANG that nourishes blood, the effect of blood circulation promoting and blood stasis dispelling;
Sargassum has eliminating phlegm and softening indurated mass, the vessel softening effect.Modern study finds that it has the effect of blood pressure lowering, blood fat reducing and anticoagulant and thrombolytic;
Radix Salviae Miltiorrhizae has blood circulation promoting and blood stasis dispelling, invigorating brain and relieving mental uneasiness effect.Modern study proves, it has expansion of cerebral vascular, promotes collateral circulation formation, cerebral blood flow increasing amount, microcirculation improvement, bring high blood pressure down, the effect of decreased heart rate;
The Radix Astragali has the effect of invigorating the spleen and replenishing QI, spleen invigorating consolidating superficial resistance.Modern study finds that it has the significant effect that improves brain blood circulation, blood pressure lowering, decreased heart rate, cerebral blood flow increasing amount;
Radix Ginseng has the effect of QI invigorating heart tonifying, spleen invigorating Fructus Alpiniae Oxyphyllae.Modern study proves, it has the central nervous system of adjustment unify endocrine metabolism and immunologic function, expansion cerebral arteries, cerebral blood flow increasing amount and the effect that brings high blood pressure down;
Pheretima have peacefully breathe heavily, the effect of dredging the meridian.Modern study finds, it has and brings high blood pressure down the effect of vessel softening;
Rhizoma Chuanxiong has activating QI to alleviate the depression, the effect of the analgesic therapy of invigorating blood circulation.Modern study finds that it has the adjustment central nervous system, expansion of cerebral vascular, microcirculation improvement and the effect that brings high blood pressure down;
Radix Angelicae Sinensis have enrich blood, the effect of promoting blood circulation and stopping pain.Modern study finds that it has the effect of adjusting nervus centralis and endocrine metabolism, improving brain blood circulation, anticoagulant;
Radix Paeoniae Rubra has the effect of removing blood stasis intestinal stasis relieving.Modern study finds that it has the effect that improves brain blood circulation, spasmolysis and analgesia and blood pressure lowering;
Flos Carthami has the effect of blood circulation promoting and blood stasis dispelling, dredging collateral to stop pain.Modern study proves that it has the effect of adjusting the central nervous system, bring high blood pressure down and improve the brain blood circulation;
Radix Puerariae has the effect of yang invigorating expelling pathogenic factors from muscles, heat clearing away spasmolytic.Modern study finds, it has improves brain blood circulation, expansion of cerebral vascular, cerebral blood flow increasing amount significantly, brings high blood pressure down and the effect of blood glucose;
Radix Et Caulis Acanthopanacis Senticosi has the invigorating middle warmer benefit smart, the effect of eliminate stagnation and dissolve phlegm.Modern study proves that it has the adaptation former state effect stronger than Radix Ginseng, can adjust cerebral cortex and vegetative nerve, strengthens resistance of human body, blood sugar lowering and blood pressure.
Radix Polygoni Multiflori has nourishing the kidney and liver, the effect of the black hair that lengthens one's life.Modern study finds that it has hypertension and hyperlipemia and function in delaying senility;
Herb Gynostemmae Pentaphylli has benefiting vital QI and blood, the strong effect that lengthens one's life.Modern study proves, it agree to have the effect of blood fat reducing and blood pressure, raising immunologic function;
Ganoderma has the effect of tranquilizing by nourishing the heart, nourishing the brain and improving intelligence.Modern study finds, it has adjusts nervus centralis, brings high blood pressure down and the effect of blood fat;
Fructus Crataegi has the effect of blood circulation promoting and blood stasis dispelling, spleen benefiting and stimulating the appetite.Modern study finds that it has the effect that improves brain blood circulation, blood fat reducing and blood pressure;
Rhizoma Gastrodiae has to relieve dizziness, high fever, infantile convulsions, epilepsy, etc. and reduces phlegm, invigorates blood circulation the effect of relieving convulsion.Modern study finds that it has the effect of adjusting nervus centralis, convulsion, ending dizziness and headache and numb limbs and tense tendons;
Fel Ursi has that resolving depression reduces phlegm, the effect of heat clearing away dissipating blood stasis.Modern study finds that it has the brain of improvement blood circulation, effect blood fat-reducing blood pressure-decreasing and that purify microecological environment in the health;
The lung benefit heart is protected in the Cordyceps the kidney invigorating.Modern study proves that it has the effect that improves immunologic function and slow down aging;
Fructus Lycii has the nourishing kidney tonifying liver, nourishing blood to tranquillize the mind, the effect that the benefit of preserving youthful looks is known.Modern study finds that it has the effect of very strong slow down aging;
Succinum has the repercussive eliminating stagnation, relieving palpitation pain relieving, the normal pulse effect of calming the nerves;
Flos Rosae Rugosae has soothing liver-QI for relieving depression, the effect of promoting flow of QI and blood.
Pharmaceutical composition of the present invention is with Sanguis Cornu Cervi Pantotrichum, Radix Ginseng, the Radix Astragali, Radix Angelicae Sinensis, Cordyceps, Fructus Lycii, Radix Polygoni Multiflori, Radix Et Caulis Acanthopanacis Senticosi, the Herb Gynostemmae Pentaphylli warming YANG that nourishes blood, and enriching yin boost qi is a principal agent.The expectorant of invigorating blood circulation of Radix Salviae Miltiorrhizae, Flos Carthami, Ganoderma, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Pheretima, Radix Puerariae, succinum, Fructus Crataegi, Sargassum, invigorating brain and relieving mental uneasiness is an accessory drugs; Flos Rosae Rugosae, Rhizoma Gastrodiae, the Fel Ursi regulating QI is soothing the liver, and clearing away heat and relieving the wind syndrome is an adjuvant.Make a general survey of full side, temperature is not and dry, and taste is and oiliness, and reinforcement and elimination in combination is association of activity and inertia, and purport is clear and definite though square medicine is assorted, is a safe and effective medicine that is suitable for taking for a long time.
The curative effect of pharmaceutical composition of the present invention is proved by following pharmacodynamic experiment:
For experiment needs easily, a kind of pharmaceutical composition of preventing and treating cardiovascular and cerebrovascular disease of the present invention is made capsule, temporarily called after brain-strengthening pulse invigorating capsule.
One, pharmaceutical composition pair cell Immune Effects of the present invention
Test unit: pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material: the brain-strengthening pulse invigorating capsule is provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.; SHANHAIDAN JIAONANG is produced by Xi'an Hong Qing pharmaceutical factory; Lymphocyte layering liquid; 0.1% sheep red blood cell (SRBC) suspension (SRBC, this chamber self-control); 1% penta 3 aldehyde solution; 0.5% U.S. blue solution.
TL-851 type G calibrates desk centrifuge, desk-top water bath (SHZ-88-1 type, granary, Jiangsu); Microscope (Olympus, Japan).
Kunming kind white mice is provided by Inner Mongol university Experimental Animal Center.
The healthy volunteer is the student of the court.
Experimental technique and result:
(1) brain-strengthening pulse invigorating capsule vivo medicine-feeding is to the influence of mice T-iss lymphocyte E--garland formation
Get 60 of Kunming mouses, body weight 18-22 gram, male and female half and half are divided into 6 groups at random, 10 every group.
(1) blank group (giving tap water);
(2)-(4) brain-strengthening pulse invigorating capsule group (to contain crude drug amount conversion 2.8,5.6,11.2g/kg);
(5)-(6) SHANHAIDAN JIAONANG group (to contain crude drug amount conversion 7.5,15g/kg), per os gavages, and gives by the 0.2ml/10g volume at every turn, once a day, serve on 7 days, after the last administration 1 hour, gets blood in eye socket, preparation lymphocyte suspension.Get 0.1ml lymphocyte suspension, add 0.1%SRBC0.1ml, mixing shakes up, 37 ℃ of water-baths 5 minutes, and 500 left the heart 5 minutes.Supernatant is removed in suction, stays a little liquid (about 0.1ml), adds 1% glutaraldehyde 0.1ml, fixes 10 minutes.Add 0.5% U.S. blue 1-2 and drip, dyeed 3-5 minute, mix gently.Get 1 and put on the microscope slide, add coverslip, high power lens is 200 lymphocytes of counting down, and 3 above persons of SRBC of surface adsorption are rosette formation cell.Calculated activity E-rosette formation cell percentage rate sees Table 1
Table 1 brain-strengthening pulse invigorating capsule is to the plastidogenetic influence of the active E-garland of mice (X ± SD)
* P＜0.05, administration group and matched group comparison.T check between group.
The result shows that the brain-strengthening pulse invigorating capsule has the effect that dose dependent improves the active E-rosette formation cell of mice production rate, has the effect that improves the mouse cell immunologic function, and its effect is strong than SHANHAIDAN JIAONANG.
(2) the brain-strengthening pulse invigorating capsule is to people's immune function influence
10 of healthy volunteers, each 5 of man, woman, age 20-22 year.
Rise morning on an empty stomach, get blood 5ml from fossa cubitalis vein respectively, preparation lymphocyte suspension.Get 70 in test tube of sterilization, 7 every group, be divided into blank pipe, the high, medium and low concentration pipe of brain-strengthening pulse invigorating capsule (concentration be respectively 0.8,0.4 and 0.2mg/ml), the high, medium and low concentration pipe of SHANHAIDAN JIAONANG (concentration be respectively 0.8,0.4 and 0.2mg/ml).Every pipe adds lymphocyte suspension 0.1ml, and for reagent liquid 0.2ml, 37 ℃ of water-baths were cultivated 30 minutes, U.S. blue dyeing, the E-rosette formation cell in smear, 200 lymphocytes of counting is obtained formation rate, and, see Table 2 by the difference that pairing T checks relatively administration and blank pipe.
The influence that table 2 brain-strengthening pulse invigorating capsule forms the In vitro culture normal person T-lymphocyte E-garland (N=10 of X ± SD)
Experimental result shows that big or middle two dosage of brain-strengthening pulse invigorating capsule all can significantly improve people's peripheral t-lymphocyte E-garland formation rate, and better with middle dose effect, and small dose group and blank group compare, no difference of science of statistics.Middle dosage and each dosage of SHANHAI PILL more also have notable difference.Illustrate that the brain-strengthening pulse invigorating capsule can improve the human body cell immunologic function.
Two, the brain-strengthening pulse invigorating capsule is to the influence of rat fat content
Test unit: pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material:
Medicine: the brain-strengthening pulse invigorating capsule is provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.;
Herb Gynostemmae Pentaphylli total glycosides tablet is produced by the Ankang pharmaceutical factory of traditional Chinese medicine;
Animal: select for use SD (Sprage Dewleg) to plant rat, body weight 200 ± 15g, male and female half and half are provided by University of the Inner Mongol's Experimental Animal Center.
Reagent: serum total cholesterol enzyme process test kit; Serum triglycerides liquid enzyme method test kit; The HDL-C test kit; More than produce by Wenzhou City east bowl biological engineering reagent instrument plant.
Equipment: 721-1 type spectrophotometer; 80-2 type centrifuge; Constant water bath box; Operating theater instruments.
Experimental technique and result:
Get 60 of normal rats, weighing makes marks, and is divided into 6 groups at random.The A group is the blank group, feeds with normal feedstuff; The B group is the hyperlipidemia model group, feeds with high lipid food (normal feedstuff+1% cholesterol+10% Adeps Sus domestica); C organizes positive matched group; Feed with high lipid food, irritate stomach simultaneously and give Herb Gynostemmae Pentaphylli total glycosides (dosage 0.5g/kg); The D group is the heavy dose of group of brain-strengthening pulse invigorating capsule (dosage 8g/kg); The E group is dosage group (dosage 4g/kg) in the brain-strengthening pulse invigorating capsule; The F group is brain-strengthening pulse invigorating capsule small dose group (dosage 2g/kg).D, E, F all feed with high lipid food for three groups, simultaneously by each group dose difference gastric infusion.
After 9 days, broken end is got blood, separation of serum.Adopt the standard reagent box, by standard method, measure blood T-CHOL, triglyceride hdl concentration, assay method is as follows:
(1) serum total cholesterol determination (enzyme process)
Have in the test kit: enzyme reagent, diluent, titer (containing cholesterol 2g/L); The enzyme working solution mixes with diluent volume with enzyme reagent at 1: 4, the according to the form below operation:
Mixing, 37 ℃ of water-baths 15 minutes are taken out to room temperature, under the 500nm wavelength, blank zeroing, 0.5cm optical path colorimetric, read to be calculated as follows after the optical density value:
The results are shown in Table 3
(2) serum triglycerides is measured (enzyme process)
Have in the test kit: enzyme reagent, diluent, triglyceride titer (2g/L), enzyme working solution use enzyme reagent and diluent with 1: 4 mixing of volume ratio, the according to the form below operation:
Mixing immediately, 37 ℃ of water-baths 15 minutes are taken out to room temperature, under the 500nm wavelength, blank zeroing, 0.5cm optical path colorimetric, read to be calculated as follows after the optical density value:
(3) serum high-density LP determination (enzyme process)
Have in the test kit: enzyme reagent, diluent, precipitant, high density lipoprotein titer (0.5g/L).Than 1: 4 mixing, operate by according to the form below with enzyme reagent and diluent volume for the enzyme working solution:
Shake up immediately, placed 15 minutes, add precipitant, add precipitant, centrifugal 15 minutes, get the supernatant mixing again with 3000 rev/mins.
37 ℃ of water-baths 15 minutes, under the 500nm wavelength, blank zeroing, 0.5cm optical path colorimetric is calculated as follows after reading optical density value:
The results are shown in Table 3.
Table 3 brain-strengthening pulse invigorating capsule is to the X ± S as a result that influences of rat fat content
T check between group: the model group normal group compares: Δ P＜0.05 Δ Δ P＜0.01
Medication group and model group compare: * p＜0.05**P＜0.01
The experiment knot shows:
The brain-strengthening pulse invigorating capsule can reduce T-CHOL in the blood, content of triglyceride, and slightly high density lipoprotein increasing content is learned according to statistics and handled, and model group and normal group compare, and cholesterol, content of triglyceride all have significantly or highly significant raises; And medication group and model group are relatively, and large, medium and small dosage is remarkable or highly significant to the reduction of T-CHOL, triglyceride in the blood; This medicine is not obvious to the effect of high density lipoprotein.
Three, strong beniol arteries and veins capsule is to the influence of White Rabbit platelet adhesion rate erythrocyte electrophoresis
Test unit:
Pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material:
Medicine: the brain-strengthening pulse invigorating capsule is provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.; Herb Gynostemmae Pentaphylli total glycosides tablet is produced by the Ankang pharmaceutical factory of traditional Chinese medicine,
Citric acid is received heparin: 150,000 units/gram, and bioid factory in Shanghai produces,
Animal: select 60 of New Zealand white rabbit for use, body weight 2.0 ± 0.3kg, male and female half and half are provided by University of the Inner Mongol's Experimental Animal Center.
Equipment: the erythrocyte electrophoresis instrument, the platelet adhesion instrument is produced by Sensitive Technology Co. of the Chinese Academy of Sciences; 80-2 type centrifuge;
Experimental technique and result:
Get 60 of normal White Rabbits, steady labelling is divided into 6 groups at random.The A group is the blank group, feeds with normal feedstuff; The B group is the hyperlipidemia model group, feeds with high lipid food (normal feedstuff+0.5% cholesterol+5% Adeps Sus domestica), and C organizes positive matched group, feeds with high lipid food, irritates stomach simultaneously and gives Herb Gynostemmae Pentaphylli total glycosides (dosage: 0.06g/kg); The D group is the heavy dose of group of brain-strengthening pulse invigorating capsule (dosage 3g/kg); The E group is dosage group (dosage 15g/kg) in the brain-strengthening pulse invigorating capsule; The F group is brain-strengthening pulse invigorating capsule small dose group (1g/kg).D, E, F all feed with high lipid food for three groups, simultaneously by each group dose gastric infusion.
After 15 days by heart extracting blood (blood drawing syringe, contain the silication in advance of blood test tube).1.2ml blood injects in the citric acid anticoagulant tube (the test tube preparation: sodium citrate is made into the solution of concentration 3.8% with distilled water, gets 0.13ml and adds in the silication test tube dry for standby), shakes up standby; 2ml blood injects anticoagulant heparin pipe (test tube preparation: heparin distilled water wiring solution-forming, contain the concentration of heparin 31.25 units with every ml blood, add bottom the test tube, put into 90-100 ℃ of baking oven dry for standby), shakes up standby.
(1) platelet adhesion rate is measured (rotary glass ball method)
Draw 1ml citric acid anticoagulation with sample injector, add in the long-neck ball vial of capacity 5ml, place it on the rotating disk of platelet adhesion instrument, take off after 15 minutes with 5 rev/mins rotating speed rotation.
Before getting the 20ml rotation respectively, blood vessel puts into 1% ammonium oxalate diluent with quantitatively getting with postrotational anticoagulation, platelet count in the blood before and after the mensuration rotation, then by following formula calculating:
The results are shown in Table 4
(2) mensuration of erythrocyte electrophoretic time
With the 2ml heparin anti-coagulating centrifugal separation plasma that takes out, centrifugal 15 minutes, rotating speed was 3000 rev/mins.
Sucking-off blood plasma is put into another test tube, draws the minute quantity erythrocyte simultaneously and puts into the blood plasma mixing, makes red cell suspension.
Red cell suspension is injected the electrophoresis pipe, measure erythrocyte needed electrophoresis time of swimming certain distance under the extra electric field effect, the results are shown in Table 4 with electrophresis apparatus.
Table 4 brain-strengthening pulse invigorating capsule is to the X ± S as a result that influences of White Rabbit platelet adhesion rate and erythrocyte electrophoresis
T check between group: model group and normal group compare: Δ P＜0.05
Each medication group and model group compare: * P＜0.05**P＜0.01
Experimental result shows:
The brain-strengthening pulse invigorating capsule can reduce platelet adhesion rate, shortens erythrocyte electrophoretic time, handles by statistics, and model group and normal group compare, and platelet adhesion rate there was no significant difference, erythrocyte electrophoretic time have significant difference (P＜0.05); And medication group and model are relatively, and large, medium and small dosage is to the reduction effect highly significant (P＜0.01) of platelet adhesion rate, and the shortening effect of big or middle dosage pair cell electrophoresis time is (p＜0.05) significantly, and low dose of no significant difference.
Four, the brain-strengthening pulse invigorating capsule is to the influence of rat thrombus in vivo formation
Test unit:
Pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material:
The brain-strengthening pulse invigorating capsule is by being provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.; SHANHAIDAN JIAONANG is produced by Xi'an Hong Qing pharmaceutical factory.
The Wistar rat is provided by University of the Inner Mongol's Experimental Animal Center.
The experimental thrombus in vivo of BT-87-2 type forms analyzer (manufacturing of medical college cardiovascular diseases research department, packet header).
Experimental technique and result:
70 of Wistar rats, body weight 180-220g; Male and female half and half are divided into 7 groups at random, 10 every group.Organize for normal saline in contrast for the 1st group; The 2-4 group is given brain-strengthening pulse invigorating capsule 2,4 and 8g/kg (calculating to contain the crude drug amount) respectively; The 5-7 group is given SHANHAIDAN JIAONANG 25.5 and 10g/kg (calculating to contain the crude drug amount) respectively; The per os gastric infusion once a day, serve on 7 days, gives by 2ml/100g at every turn.After the last administration 1 hour, behind pentobarbital sodium 45mg/kg intraperitoneal injection of anesthesia, separate a side common carotid artery by electrostimulation, the stimulating electrode that forms instrument with experimental thrombosis stimulates common carotid artery entad to hold (electric current 1.5mA, stimulation time is 7 minutes), survey blood vessel distal end surface temperature with temperature probe, observe common carotid artery bloodstream blocking time (occlusion time, and calculate the rate elongation of administration group OT OT):
Experimental result shows: thrombus formation time in the brain-strengthening pulse invigorating capsule concentration dependent ground extension body, heavy dose of group relatively has notable difference with matched group.Though each organizes the trend that also has prolongation OT value SHANHAIDAN JIAONANG, each group and the relatively more equal not statistically significant of matched group.The results are shown in Table 5.
The influence that table 5 brain-strengthening pulse invigorating capsule forms the rat thrombus in vivo (X ± SD)
* P＜0.05, T check between administration group and matched group comparable group
Five, the brain-strengthening pulse invigorating capsule is to the not influence of the property finished cerebral ischemia of rat acute
Test unit:
Pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material:
The brain-strengthening pulse invigorating capsule is provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.; SHANHAIDAN JIAONANG is produced by Xi'an Hong Qing pharmaceutical factory.
The Wistar rat is provided by the big word Experimental Animal Center in the Inner Mongol.
The T-6328A photoelectric analytical balance, analytical tool factory in Shanghai makes.
Experimental technique and result:
80 of Wistar rats, body weight 205 ± 24g, male and female half and half are divided into 8 groups at random, 10 every group.(1) blank group; (2) the cerebral ischemic model group is a negative control group.Above-mentioned two groups gavage normal saline 2ml/100g/d; (3)-(5) be big-and-middle, the small dose group of brain-strengthening pulse invigorating capsule, gavage 2,4 respectively, 8g/kg/d (by containing the crude drug amount); (6)-(8) be the large, medium and small dosage group of SHANHAIDAN JIAONANG, gavage 2.5,5 respectively, 10g/kg/d (by containing the crude drug amount).It is the same that the administration group is irritated the stomach volume, serve on 7 days.The method of reference literature record is given back 1 hour in last, after animal is used sodium pentobarbital (45mg/kg) intraperitoneal injection of anesthesia, separate bilateral common carotid arteries, introduce two-wire ligation (a blank group threading, not ligation), cause the cerebral ischemia of acute experiment imperfection, after the ligation 3 hours, quick broken end is got brain and is claimed weight in wet base, calculates cerebral index, dries by the fire 48 hours in 110 ℃ of baking boxs then, claim dry weight, calculate brain water content.
Cerebral index=(cutaneous horn heavy * 100%)/body weight
Table 6 brain-strengthening pulse invigorating capsule is to the influence of rat brain index, brain water content (X ± SD)
* P＜0.05, model group and blank group relatively, T check between group;
* T check between P＜0.05 * * P＜0.01 administration group and model group comparable group
Experimental result shows that the cerebral index of cerebral ischemic model group, brain water content increase than the blank group, and learning by statistics to handle has significant difference, illustrates that cerebral ischemic model is successful.In the brain-strengthening pulse invigorating capsule, heavy dose of group all has significant difference with ischemia model group comparison cerebral index, brain water content, heavy dose of group is similar to the blank group, can alleviate the caused cerebral edema of acute experiment imperfection cerebral ischemia with the similar brain-strengthening pulse invigorating capsule that shows of SHANHAIDAN JIAONANG.
Six, the brain-strengthening pulse invigorating capsule is to the influence of chmice acute cerebral ischemia
Test unit:
Pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material:
The brain-strengthening pulse invigorating capsule is provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.; SHANHAIDAN JIAONANG is produced by Xi'an Hong Qing pharmaceutical factory;
The kind white mice is provided by University of the Inner Mongol's Experimental Animal Center in Kunming.
Experimental technique and result:
Get 120 of mices, body weight 18-22g, male and female half and half are divided into 6 groups at random, 20 every group.(1) blank group (giving tap water); (2)-(4) brain-strengthening pulse invigorating capsule group (to contain crude drug amount conversion 2.8,5.6,11.2g/kg) to contain crude drug; (5)-(6) SHANHAIDAN JIAONANG group (7.5,15g/kg calculate) to contain the crude drug amount, each 0.2ml/10g volume gastric infusion of pressing, once a day, continuous 7 days, after the last administration 1 hour, break end rapidly at the mouse ear rear portion, cause the brain acute ischemia, observe frequency of respiration and the breathing of broken end back animal and hold time, the results are shown in Table 7.
* P＜0.05; * P＜0.01, administration group and matched group are relatively, T check between group. experimental result shows, the brain-strengthening pulse invigorating capsule has the dose-effect dependency along with frequency of respiration and breathing that the dosage increase can obviously increase decapitated mice are held time. show that the brain-strengthening pulse invigorating capsule can resist chmice acute cerebral hemorrhage, anoxia symptom.
Seven, the brain-strengthening pulse invigorating capsule is to the influence of mice swimming time
Test unit:
Pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material:
The brain-strengthening pulse invigorating capsule is provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.; SHANHAIDAN JIAONANG Xi'an Hong Qing pharmaceutical factory provides.
The kind white mice is provided by University of the Inner Mongol's Experimental Animal Center in Kunming.
Experimental technique and result:
60 of Kunming kind white mice, body weight 22 ± 3g, male and female half and half are divided into 6 groups at random, 10 every group.
(1) the blank group gives tap water; (2)-(4) brain-strengthening pulse invigorating capsule group (2.8,5.6 and 11.2g/kg, calculate to contain the crude drug amount) (5)-(6) SHANHAIDAN JIAONANG group (7.5 and 15.0g/kg, calculate to contain the crude drug amount) press gastric infusion 0.2ml/10g/ time, once a day, continuous eight days, after the last administration one hour, mice is placed on swimming in the glass jar (20 ± 0.5 ℃ of depth of water 20cm, water temperatures) respectively, observe the mice swimming time and relatively organize a T inspection with matched group.The results are shown in Table 8.
The result shows: brain-strengthening pulse invigorating capsule dependency prolongs the mice swimming time, and the SHANHAIDAN JIAONANG of more similar dosage obviously strengthens.
Table 8 brain-strengthening pulse invigorating capsule is to the influence of mice swimming time (X ± SD)
* P＜0.05; * P＜0.01, the more identical T check of administration group with matched group
Δ Δ P＜0.01, the heavy dose of group of brain-strengthening pulse invigorating capsule is organized relatively with SHANHAIDAN JIAONANG is heavy dose of, T check between group
Eight, the brain-strengthening pulse invigorating capsule is to the influence of mice normal pressure resistant anoxia
Test unit: pharmaceutical college of Medical Colleges of the Inner Mongol Pharmacology Lab
Experiment material: the brain-strengthening pulse invigorating capsule is provided by Hulunbeier Songlu Pharmaceutical Co., Ltd.; SHANHAIDAN JIAONANG Xi'an Hong Qing pharmaceutical factory produces.The kind white mice is provided by University of the Inner Mongol's Experimental Animal Center in Kunming.
Method and result:
60 of Kunming kind white mice, body weight 22 ± 3g, male and female half and half are divided into 6 groups at random, 10 every group.(1) the blank group gives tap water; (2)-(4) brain-strengthening pulse invigorating capsule group (2.8,5.6 and 11.2g/kg), calculate to contain the crude drug amount) (5)-(6) SHANHAIDAN JIAONANG group (7.5 and 15.0g/kg, calculate to contain the crude drug amount) press gastric infusion 0.2ml/10g/ time, once a day, continuous eight days, after the last administration one hour, mice is put into the wide mouthed bottle (putting 1 for every bottle) that fills the 15g sodica calx, cover completely and make it air tight, timing immediately.With the respiratory arrest is index, observes mice because of the dead time of anoxia.Relatively carry out statistical procedures state with matched group.The results are shown in Table 3.
The result shows: heavy dose of brain-strengthening pulse invigorating capsule can obviously prolong the time-to-live of mice under the normobaric hypoxia condition.
Table 9 brain-strengthening pulse invigorating capsule is to the influence of mice normal pressure resistant anoxia (X ± SD)
* P＜0.05, T check between administration group and matched group comparable group
Experiment conclusion:
Experimental result shows that the brain-strengthening pulse invigorating capsule confirms the cellular immunology test of inside and outside, can improve mice and people T-lymphocyte E-garland formation rate, shows that it can improve body's immunological function.Hyperlipidemia model experiment prompting, this medicine can reduce T-CHOL in the blood, content of triglyceride, slightly high density lipoprotein increasing content; And can reduce platelet adhesion rate, shorten the erythrocyte electricity trip time.The brain-strengthening pulse invigorating capsule is formed with the obvious suppression effect to the rat thrombus in vivo, its effect is strong than SHANHAI PILL, to in the acute cerebral ischemia in rats, heavy dose of group can reduce brain water content, and the frequency of respiration and the breathing that can prolong the chmice acute cerebral ischemia are held time, this medicine can also prolong the time of swimming time and the normal pressure resistant anoxia of mice, illustrates that it can alleviate physical work fatigue and increase the anaerobic condition that oxygen supply improves body.
The specific embodiment
Embodiment 1
(1) it is standby to take by weighing Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 3g Radix Ginseng 1g Radix Astragali 40g Radix Angelicae Sinensis 9g Cordyceps 1g Fructus Lycii 10g Radix Polygoni Multiflori (system) 23g Radix Et Caulis Acanthopanacis Senticosi 10g Herb Gynostemmae Pentaphylli 10g Radix Salviae Miltiorrhizae 20g Flos Carthami 10g Ganoderma 1g Radix Paeoniae Rubra 10g Rhizoma Chuanxiong 23g Pheretima 10g Radix Puerariae 23 succinum 1g Fructus Crataegi 10g Sargassum 10g Flos Rosae Rugosae 2g Rhizoma Gastrodiae 5g Fel Ursi 1g;
(2) getting the Radix Astragali in the above-mentioned prescription, Radix Puerariae, Flos Carthami, Herb Gynostemmae Pentaphylli, Rhizoma Chuanxiong, Flos Rosae Rugosae, Sargassum, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Pheretima, Fructus Crataegi, Rhizoma Gastrodiae, Radix Polygoni Multiflori ten Six-element medical materials gives as one thinks fit cataclasm;
(3) above-mentioned cataclasm medical material is put in the extraction pot, added 4 times of 75% ethanol amount dipping 12 hours, the heating and refluxing extraction secondary, each 2 hours, merge extractive liquid, was put coldly, filters, and decompression recycling ethanol to relative density is the clear paste of 1.10-1.20 (60 ℃), and is standby;
(4) medicinal residues that above-mentioned ethanol extraction is crossed add 8 times of amounts of water, the heating and refluxing extraction secondary, and each 2 hours, merge extractive liquid, filters, and is concentrated into relative density and is the clear paste of 1.10-1.20 (60 ℃), and was standby;
(5) with above-mentioned (3) and (4) clear paste of extracting mix, dry in vacuum desiccator, get dry extract;
(6) give as one thinks fit cataclasm and dried cream to mix pulverizing all the other Six-element medical materials such as Sanguis Cornu Cervi Pantotrichum, cross 100 mesh sieves, make granule, incapsulate, promptly.
Embodiment 2
(1) it is standby to take by weighing Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 4g Radix Ginseng 2g Radix Astragali 42g Radix Angelicae Sinensis 10g Cordyceps 2g Fructus Lycii 11g Radix Polygoni Multiflori (system) 24g Radix Et Caulis Acanthopanacis Senticosi 11g Herb Gynostemmae Pentaphylli 11g Radix Salviae Miltiorrhizae 25g Flos Carthami 11g Ganoderma 2g Radix Paeoniae Rubra 11g Rhizoma Chuanxiong 24 Pheretima 11g Radix Puerariae 24g succinum 2g Fructus Crataegi 11g Sargassum 11g Flos Rosae Rugosae 3g Rhizoma Gastrodiae 6g Fel Ursi 2g;
(2) removing the Radix Astragali in the above-mentioned prescription, Radix Puerariae, Flos Carthami, Herb Gynostemmae Pentaphylli, Rhizoma Chuanxiong, Flos Rosae Rugosae, Sargassum, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Pheretima, Fructus Crataegi, Rhizoma Gastrodiae, Radix Polygoni Multiflori ten Six-element medical materials gives as one thinks fit cataclasm;
(3) above-mentioned cataclasm medical material is put in the extraction pot, added 4 times of 80% ethanol amount dipping 12 hours, the heating and refluxing extraction secondary, each 2 hours, merge extractive liquid, was put coldly, filters, and decompression recycling ethanol to relative density is the clear paste of 1.10-1.20 (60 ℃), and is standby;
(4) medicinal residues that above-mentioned ethanol extraction is crossed add 9 times of amounts of water, the heating and refluxing extraction secondary, and each 2 hours, merge extractive liquid, filters, and is concentrated into relative density and is the clear paste of 1.10-1.20 (60 ℃), and was standby;
(5) with above-mentioned (3) and (4) clear paste of extracting mix, dry in vacuum desiccator, get dry extract;
(6) give as one thinks fit cataclasm and dried cream to mix pulverizing all the other Six-element medical materials such as Sanguis Cornu Cervi Pantotrichum, cross 100 mesh sieves, make granule, make tablet by the manufacture method of tablet, promptly.
Embodiment 3
It is standby to take by weighing Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 5g Radix Ginseng 3g Radix Astragali 45g Radix Angelicae Sinensis 12g Cordyceps 3g Fructus Lycii 12g Radix Polygoni Multiflori (system) 26g Radix Et Caulis Acanthopanacis Senticosi 12g Herb Gynostemmae Pentaphylli 12g Radix Salviae Miltiorrhizae 30g Flos Carthami 13g Ganoderma 3g Radix Paeoniae Rubra 12g Rhizoma Chuanxiong 27g Pheretima 12g Radix Puerariae 27g succinum 3g Fructus Crataegi 12g Sargassum 12g Flos Rosae Rugosae 5g Rhizoma Gastrodiae 7g Fel Ursi 3g;
2) getting the Radix Astragali in the above-mentioned prescription, Radix Puerariae, Flos Carthami, Herb Gynostemmae Pentaphylli, Rhizoma Chuanxiong, Flos Rosae Rugosae, Sargassum, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Pheretima, Fructus Crataegi, Rhizoma Gastrodiae, Radix Polygoni Multiflori ten Six-element medical materials gives as one thinks fit cataclasm;
(3) above-mentioned cataclasm medical material is put in the extraction pot, added 4 times of 85% ethanol amount dipping 12 hours, the heating and refluxing extraction secondary, each 2 hours, merge extractive liquid, was put coldly, filters, and decompression recycling ethanol to relative density is the clear paste of 1.10-1.20 (60 ℃), and is standby;
(4) medicinal residues that above-mentioned ethanol extraction is crossed add 10 times of amounts of water, the heating and refluxing extraction secondary, and each 2 hours, merge extractive liquid, filters, and is concentrated into relative density and is the clear paste of 1.10-1.20 (60 ℃), and was standby;
(5) with above-mentioned (3) and (4) clear paste of extracting mix, dry in vacuum desiccator, get dry extract;
(6) give as one thinks fit cataclasm and dried cream to mix pulverizing all the other Six-element medical materials such as Sanguis Cornu Cervi Pantotrichum, cross 100 mesh sieves, make soft capsule by the soft capsule manufacture method, promptly.
Embodiment 4
1g is standby for Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 5g Radix Ginseng 2g Radix Astragali 43g Radix Angelicae Sinensis 12g Cordyceps 3g Fructus Lycii 12g Radix Polygoni Multiflori (system) 25g Radix Et Caulis Acanthopanacis Senticosi 11g Herb Gynostemmae Pentaphylli 11g Radix Salviae Miltiorrhizae 30g Flos Carthami 13g Ganoderma 3g Radix Paeoniae Rubra 12g Rhizoma Chuanxiong 26g Pheretima 11g Radix Puerariae 25g succinum 2g Fructus Crataegi 11g Sargassum 11g Flos Rosae Rugosae 4g Rhizoma Gastrodiae 5g Fel Ursi;
2) getting the Radix Astragali in the above-mentioned prescription, Radix Puerariae, Flos Carthami, Herb Gynostemmae Pentaphylli, Rhizoma Chuanxiong, Flos Rosae Rugosae, Sargassum, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Pheretima, Fructus Crataegi, Rhizoma Gastrodiae, Radix Polygoni Multiflori ten Six-element medical materials gives as one thinks fit cataclasm;
(3) above-mentioned cataclasm medical material is put in the extraction pot, added 4 times of 75% ethanol amount dipping 12 hours, the heating and refluxing extraction secondary, each 2 hours, merge extractive liquid, was put coldly, filters, and decompression recycling ethanol to relative density is the clear paste of 1.10-1.20 (60 ℃), and is standby;
(4) medicinal residues that above-mentioned ethanol extraction is crossed add 8 times of amounts of water, the heating and refluxing extraction secondary, and each 2 hours, merge extractive liquid, filters, and is concentrated into relative density and is the clear paste of 1.10-1.20 (60 ℃), and was standby;
(5) with above-mentioned (3) and (4) clear paste of extracting mix, dry in vacuum desiccator, get dry extract;
(6) give as one thinks fit cataclasm and dried cream to mix pulverizing all the other Six-element medical materials such as Sanguis Cornu Cervi Pantotrichum, cross 100 mesh sieves, make drop pill by the drop pill manufacture method, promptly.
Embodiment 5
It is standby to take by weighing Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 4g Radix Ginseng 2g Radix Astragali 43g Radix Angelicae Sinensis 11g Cordyceps 2g Fructus Lycii 11g Radix Polygoni Multiflori (system) 25g Radix Et Caulis Acanthopanacis Senticosi 11g Herb Gynostemmae Pentaphylli 11g Radix Salviae Miltiorrhizae 25g Flos Carthami 11g Ganoderma 2g Radix Paeoniae Rubra 11g Rhizoma Chuanxiong 25g Pheretima 11g Radix Puerariae 25g succinum 2g Fructus Crataegi 11g Sargassum 11g Flos Rosae Rugosae 4g Rhizoma Gastrodiae 6g Fel Ursi 2g Rhizoma Gastrodiae 5-7g Fel Ursi 1-3g;
2) getting the Radix Astragali in the above-mentioned prescription, Radix Puerariae, Flos Carthami, Herb Gynostemmae Pentaphylli, Rhizoma Chuanxiong, Flos Rosae Rugosae, Sargassum, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Pheretima, Fructus Crataegi, Rhizoma Gastrodiae, Radix Polygoni Multiflori ten Six-element medical materials gives as one thinks fit cataclasm;
(3) above-mentioned cataclasm medical material is put in the extraction pot, added 4 times of 75% ethanol amount dipping 12 hours, the heating and refluxing extraction secondary, each 2 hours, merge extractive liquid, was put coldly, filters, and decompression recycling ethanol to relative density is the clear paste of 1.10-1.20 (60 ℃), and is standby;
(4) medicinal residues that above-mentioned ethanol extraction is crossed add 8 times of amounts of water, the heating and refluxing extraction secondary, and each 2 hours, merge extractive liquid, filters, and is concentrated into relative density and is the clear paste of 1.10-1.20 (60 ℃), and was standby;
(5) with above-mentioned (3) and (4) clear paste of extracting mix, dry in vacuum desiccator, get dry extract;
(6) give as one thinks fit cataclasm and dried cream to mix pulverizing all the other Six-element medical materials such as Sanguis Cornu Cervi Pantotrichum, cross 100 mesh sieves, make granule, make granule by the granule manufacture method, promptly.
Claims (5)
1. prevent and treat pharmaceutical composition of cardiovascular and cerebrovascular disease and preparation method thereof for one kind, it is characterized in that being prepared from by the bulk drug processing of following weight parts: antler blood (deer blood) the 3-5g ginseng 1-3g Radix Astragali 40-45g Radix Angelicae Sinensis 9-12g Cordyceps sinensis 1-3g fruit of Chinese wolfberry 10-12g fleece-flower root (system) 23-26g wilsonii 10-12g gynostemma pentaphylla 10-12g red sage root 20-30g safflower 10-13g glossy ganoderma 1-3g radix paeoniae rubrathe 10-12g Ligusticum wallichii 23-27g earthworm 10-12g root of kudzu vine 23-27g amber 1-3g hawthorn 10-12g marine alga 10-12g rose 2-5g rhizoma Gastrodiae 5-7g bear gall 1-3g.
2. pharmaceutical composition as claimed in claim 1 is characterized in that becoming the optimum scheme by the following raw medicaments in portion by weight processing and preparing: Sanguis Cornu Cervi Pantotrichum (Sanguis cervi) 4g Radix Ginseng 2g Radix Astragali 43g Radix Angelicae Sinensis 11g Cordyceps 2g Fructus Lycii 11g Radix Polygoni Multiflori (system) 25g Radix Et Caulis Acanthopanacis Senticosi 11g Herb Gynostemmae Pentaphylli 11g Radix Salviae Miltiorrhizae 25g Flos Carthami 11g Ganoderma 2g Radix Paeoniae Rubra 11g Rhizoma Chuanxiong 25g Pheretima 11g Radix Puerariae 25g succinum 2g Fructus Crataegi 11g Sargassum 11g Flos Rosae Rugosae 4g Rhizoma Gastrodiae 6g Fel Ursi 2g.
3. preparation method as claimed in claim 1 is characterized in that being prepared into capsule and comprises following preparation steps:
(1) it is standby to take by weighing in above-mentioned 1 or 2 crude drug in proportion;
(2) getting the Radix Astragali, Radix Puerariae, Flos Carthami, Herb Gynostemmae Pentaphylli, Rhizoma Chuanxiong, Flos Rosae Rugosae, Sargassum, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Pheretima, Fructus Crataegi, Rhizoma Gastrodiae, Radix Polygoni Multiflori ten Six-element medical materials gives as one thinks fit cataclasm;
(3) above-mentioned cataclasm medical material is put in the extraction pot, added 4 times of amount dippings of 75-85% ethanol 12 hours, the heating and refluxing extraction secondary, each 2 hours, merge extractive liquid, was put cold, filter, decompression recycling ethanol to relative density is the clear paste of 1.10-1.20 (60 ℃), and is standby;
(4) medicinal residues that above-mentioned ethanol extraction is crossed add water 8-10 and doubly measure, the heating and refluxing extraction secondary, and each 2 hours, merge extractive liquid, filters, and is concentrated into relative density and is the clear paste of 1.10-1.20 (60 ℃), and was standby;
(5) with above-mentioned (3) and (4) clear paste of extracting mix, dry in vacuum desiccator, get dry extract;
(6) give as one thinks fit cataclasm and dried cream to mix pulverizing all the other Six-element medical materials such as Sanguis Cornu Cervi Pantotrichum, cross 100 mesh sieves, make granule, incapsulate, promptly.
4. as preparation method as described in the claim 3, it is characterized in that medicated powder of crossing 100 mesh sieves in (6) can be made tablet, granule, drop pill, soft capsule dosage form by the preparation method preparation of tablet, granule, drop pill, soft capsule dosage form.
5. pharmaceutical composition as claimed in claim 1 is characterized in that its function has the adjusting neuroendocrine system; Improve brain blood material circulation, blood fat reducing, blood glue, and improve brain function, control cerebrovascular and function in delaying senility.
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| CN103385963A (en) * | 2013-07-23 | 2013-11-13 | 于军 | Chinese medicine preparation for treating angina pectoris caused by coronary heart disease |
| CN103385963B (en) * | 2013-07-23 | 2014-08-13 | 于军 | Chinese medicine preparation for treating angina pectoris caused by coronary heart disease |
| CN103520257A (en) * | 2013-10-11 | 2014-01-22 | 李林 | Safflower composite medicine and application thereof |
| CN105106438A (en) * | 2015-09-23 | 2015-12-02 | 青岛友诚高新技术有限公司 | Traditional Chinese medicine composition for treating arrhythmia and preparation method thereof |
| CN105456710A (en) * | 2016-01-07 | 2016-04-06 | 孟寒 | Blood-activating and lipid-lowering medicine and preparation method thereof |
| CN106138759A (en) * | 2016-08-29 | 2016-11-23 | 姚新亮 | A kind of pharmaceutical composition preventing and treating cardiovascular and cerebrovascular disease |
| CN106822616A (en) * | 2017-04-05 | 2017-06-13 | 李少伦 | It is a kind of to treat medicine of cardiovascular and cerebrovascular diseases and preparation method thereof |
| CN109423403A (en) * | 2017-08-21 | 2019-03-05 | 天津市神草医药动物养殖有限公司 | The manufacturing method and its production technology of fine and soft perfume liquid |
| CN107970380A (en) * | 2018-01-18 | 2018-05-01 | 邵文通 | A kind of Chinese medicine preparation for preventing cardio-cerebral diseases and its preparation |
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