CN102144546A - Method for subculturing camphorwood tissue culture seedlings - Google Patents
Method for subculturing camphorwood tissue culture seedlings Download PDFInfo
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- CN102144546A CN102144546A CN 201110004918 CN201110004918A CN102144546A CN 102144546 A CN102144546 A CN 102144546A CN 201110004918 CN201110004918 CN 201110004918 CN 201110004918 A CN201110004918 A CN 201110004918A CN 102144546 A CN102144546 A CN 102144546A
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Abstract
The invention provides a method for subculturing camphorwood tissue culture seedlings. The formulation of a subculture medium for the culture method comprises 1/2 MS of culture medium, 2.5-4 mg/L of 6-BA (6-Benzyl Aminopurine), 0.1-0.5 mg/L of NAA (Naphthyl Acetic Acid), 6.5 g/L agar and 30 g/L of sucrose. Multiple subcultures are carried out by using the same subculture formulation, the multiplication factor can be increased to achieve the purpose of propagation, industrial production can be carried out, and a great number of nursery stocks are provided for the market. The method for subculturing the camphorwood tissue culture seedlings, provided by the invention, is beneficial to not only simplification of the operating process, but also reduction of the production cost, thereby achieving higher economic benefits.
Description
Technical field
The present invention relates to a kind of successive transfer culture method of fragrant camphor tree tissue cultivating seedling.
Background technology
Lignum cinnamomi camphorae material texture is careful, pliable and tough densification, and smooth and beautiful appearance contains volatile oil and special fragrance, moisture-proof, anti-corruption, the worm of dispelling, little, the long shelf-life of distortion, is valuable furniture, building, bin, engraving, shipbuilding good timber.Root, branch, leaf, timber all can extract camphor, camphor oil, and camphor, camphor oil are the important source material of aspects such as modern age medicine, metallurgy, chemical industry, spices, food industry and military project, be that China south is precious with material with specially use economic tree, but the differentiation of seed seedling seriously.Vegetative propagation can keep maternal merit, as the group culturation rapid propagating technology of nursery stock, though some researchs are arranged in theory, still exists many problems in tissue culture procedures and batch production production, and in the successive transfer culture process, growth coefficient is low; The prescription difference of successive transfer culture repeatedly; Repeatedly behind the subculture, value-added coefficient reduces bigger.
Summary of the invention
Produce the problem that exists for solving present fragrant camphor tree batch production, low as value-added coefficient, repeatedly the subculture prescription is different, and the subculture increment problem of easily degenerating repeatedly the invention provides a kind of successive transfer culture method of fragrant camphor tree tissue cultivating seedling.
The successive transfer culture method of fragrant camphor tree tissue cultivating seedling of the present invention, its successive transfer culture based formulas is: 1/2MS(macroelement consumption reduces by half)+6-BA 2.5～4mg/L+NAA 0.1～0.5 mg/L+ agar 6.5g/L+ sucrose 30 g/L.Preferably, described successive transfer culture based formulas is: 1/2MS+6-BA3.0 mg/L+NAA0.4 mg/L+ agar 6.5g/L+ sucrose 30g/L.
The step of described successive transfer culture method is: after obtaining the nontoxic seedling of fragrant camphor tree, when axillalry bud grows to 1.5-2.0cm, downcut axillalry bud from explant, with axillalry bud switching carrying out on described subculture medium successive transfer culture.Repeatedly successive transfer culture adopts with a kind of subculture medium.
Utilize successive transfer culture method of the present invention, the subculture number of successive transfer culture can reach more than 15 times.
Remarkable advantage of the present invention:
The present invention filters out the higher culture medium prescription of a kind of value-added coefficient, and repeatedly subculture can improve value-added coefficient to carry out with a kind of subculture prescription, reaches to expand numerous purpose, can carry out batch production production, for market provides a large amount of nursery stocks.The successive transfer culture method of fragrant camphor tree tissue cultivating seedling of the present invention not only can simplify the operation course, and can reduce production costs, and reaches higher economic benefit.
Description of drawings
Fig. 1 is 8.1 fragrant camphor tree tissue cultivating seedling for value-added coefficient.
Embodiment
1, medium screening
Fragrant camphor tree tissue cultivating seedling is through behind the initial culture, and axillalry bud grows to 1.5-2.0cm, downcuts axillalry bud from explant, and axillalry bud is transferred on subculture medium.
L9(3 is adopted in the screening of subculture experiment culture medium prescription
4) Orthogonal Experiment and Design, as shown in table 1, the basic element of cell division is 6-BA, growth hormone is NAA, IBA.Inoculate after 30 days, effective bud number of the bud that statistics can subculture is as the method for estimating value-added coefficient.
The Orthogonal Experiment and Design of the fragrant camphor tree subculture medium screening of table 1
Fragrant camphor tree tissue cultivating seedling is through behind the successive transfer culture, filtering out best successive transfer culture based formulas with orthogonal design method is: the MS medium of the improvement that macroelement reduces by half+6-BA3.0 mg/L+NAA0.4 mg/L+ agar 6.5g/L+ sucrose 30g/L, value-added coefficient is up to more than 6.As shown in Figure 1, adopt with a kind of subculture medium, through 2 successive transfer culture, fragrant camphor tree tissue cultivating seedling value-added coefficient is 8.1.The general growth coefficient of prior art is less than 3, and differently do not have system and need different propagation prescriptions, exists a lot of problems as subculture continuously simultaneously, easily produces vitrifying seedling etc.The present invention uses with a kind of culture medium prescription all the time in the successive transfer culture process, can reduce the probability of variation like this, and can reduce production costs, and shortens the production cycle.
Claims (4)
1. the successive transfer culture method of a fragrant camphor tree tissue cultivating seedling, it is characterized in that: the successive transfer culture based formulas of fragrant camphor tree tissue cultivating seedling is: 1/2MS medium+6-BA 2.5-4mg/L+NAA 0.1-0.5 mg/L+ agar 6.5g/L+ sucrose 30 g/L.
2. the successive transfer culture method of fragrant camphor tree tissue cultivating seedling according to claim 1 is characterized in that: the successive transfer culture based formulas of fragrant camphor tree tissue cultivating seedling is: 1/2MS+6-BA3.0 mg/L+NAA0.4 mg/L+ agar 6.5g/L+ sucrose 30g/L.
3. the successive transfer culture method of fragrant camphor tree tissue cultivating seedling according to claim 1 and 2, it is characterized in that: the step of described successive transfer culture method is: after obtaining the nontoxic seedling of fragrant camphor tree, when axillalry bud grows to 1.5-2.0cm, downcut axillalry bud from explant, with axillalry bud switching on described subculture medium, carrying out successive transfer culture.
4. the successive transfer culture method of fragrant camphor tree tissue cultivating seedling according to claim 1 and 2 is characterized in that: repeatedly successive transfer culture adopts with a kind of subculture medium.
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| CN201110004918XA CN102144546B (en) | 2011-01-12 | 2011-01-12 | Method for subculturing camphorwood tissue culture seedlings |
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| CN201110004918XA CN102144546B (en) | 2011-01-12 | 2011-01-12 | Method for subculturing camphorwood tissue culture seedlings |
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| CN102144546A true CN102144546A (en) | 2011-08-10 |
| CN102144546B CN102144546B (en) | 2012-04-25 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511270A (en) * | 2011-12-09 | 2012-06-27 | 大千生态景观股份有限公司 | Method for planting Cinnamomum camphora in north |
| CN102860258A (en) * | 2012-09-19 | 2013-01-09 | 广东省林业科学研究院 | Clonal tissue culture breeding method for camphor tree |
| CN103651145A (en) * | 2013-12-26 | 2014-03-26 | 江西省林业科学院 | Cinnamomum micranthum tissue culture method |
| CN103782906A (en) * | 2013-12-30 | 2014-05-14 | 宁波城市职业技术学院 | Method for regenerating camphor variety yongjin (Chinese character) plant |
| CN103947556A (en) * | 2014-05-06 | 2014-07-30 | 南阳师范学院 | Method for selecting adult state potential in-vitro flowering materials of cinnamomumcamphoraL. and regenerating plant |
| CN103947555A (en) * | 2014-05-06 | 2014-07-30 | 南阳师范学院 | Clonal breeding method for regenerating high-frequency plants of cinnamomumcamphoraL. embryo culture seedling stem segment |
| CN105941150A (en) * | 2016-05-20 | 2016-09-21 | 宁波城市职业技术学院 | Method for germinating and propagating cinnamomum camphora 'Yongjin' clusters |
| CN106942059A (en) * | 2017-03-27 | 2017-07-14 | 河池乐康生态农业科技有限公司 | A kind of method for tissue culture of cinnamomum camphora |
| CN108934700A (en) * | 2018-07-05 | 2018-12-07 | 紫云自治县紫香源农林科技有限责任公司 | A kind of mating system of cinnamomum camphora tree |
| CN110771505A (en) * | 2019-11-19 | 2020-02-11 | 南昌工程学院 | Culture method of citral tonglu stem tissue |
| CN111316919A (en) * | 2020-04-22 | 2020-06-23 | 安徽农业大学 | Method for improving regeneration efficiency in cinnamomum camphora tissue culture process |
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| CN101933455A (en) * | 2009-07-03 | 2011-01-05 | 中国科学院上海生命科学研究院 | In vitro propagation method for cinnamomum japonicum |
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| CN101933455A (en) * | 2009-07-03 | 2011-01-05 | 中国科学院上海生命科学研究院 | In vitro propagation method for cinnamomum japonicum |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511270A (en) * | 2011-12-09 | 2012-06-27 | 大千生态景观股份有限公司 | Method for planting Cinnamomum camphora in north |
| CN102511270B (en) * | 2011-12-09 | 2013-12-04 | 大千生态景观股份有限公司 | Method for planting Cinnamomum camphora in north |
| CN102860258A (en) * | 2012-09-19 | 2013-01-09 | 广东省林业科学研究院 | Clonal tissue culture breeding method for camphor tree |
| CN102860258B (en) * | 2012-09-19 | 2014-05-07 | 广东省林业科学研究院 | Clonal tissue culture breeding method for camphor tree |
| CN103651145A (en) * | 2013-12-26 | 2014-03-26 | 江西省林业科学院 | Cinnamomum micranthum tissue culture method |
| CN103651145B (en) * | 2013-12-26 | 2016-07-06 | 江西省林业科学院 | A kind of heavy water Camphor tree method for tissue culture |
| CN103782906A (en) * | 2013-12-30 | 2014-05-14 | 宁波城市职业技术学院 | Method for regenerating camphor variety yongjin (Chinese character) plant |
| CN103782906B (en) * | 2013-12-30 | 2016-03-30 | 宁波城市职业技术学院 | A kind of fragrant camphor tree kind gushes the method for golden plant regeneration |
| CN103947556B (en) * | 2014-05-06 | 2016-03-02 | 南阳师范学院 | The selection of the potential test tube flowering material of a kind of fragrant camphor tree Adult form and plant regeneration method |
| CN103947555A (en) * | 2014-05-06 | 2014-07-30 | 南阳师范学院 | Clonal breeding method for regenerating high-frequency plants of cinnamomumcamphoraL. embryo culture seedling stem segment |
| CN103947556A (en) * | 2014-05-06 | 2014-07-30 | 南阳师范学院 | Method for selecting adult state potential in-vitro flowering materials of cinnamomumcamphoraL. and regenerating plant |
| CN103947555B (en) * | 2014-05-06 | 2016-08-17 | 南阳师范学院 | Radix Cinnamomi porrecti embryo seedlings cultivating cutting shoots plant regeneration Clone Selection And Reproduction method |
| CN105941150A (en) * | 2016-05-20 | 2016-09-21 | 宁波城市职业技术学院 | Method for germinating and propagating cinnamomum camphora 'Yongjin' clusters |
| CN105941150B (en) * | 2016-05-20 | 2018-06-15 | 宁波城市职业技术学院 | Cinnamomum camphora gushes golden adventitious buds proliferation method |
| CN106942059A (en) * | 2017-03-27 | 2017-07-14 | 河池乐康生态农业科技有限公司 | A kind of method for tissue culture of cinnamomum camphora |
| CN108934700A (en) * | 2018-07-05 | 2018-12-07 | 紫云自治县紫香源农林科技有限责任公司 | A kind of mating system of cinnamomum camphora tree |
| CN110771505A (en) * | 2019-11-19 | 2020-02-11 | 南昌工程学院 | Culture method of citral tonglu stem tissue |
| CN111316919A (en) * | 2020-04-22 | 2020-06-23 | 安徽农业大学 | Method for improving regeneration efficiency in cinnamomum camphora tissue culture process |
| CN111316919B (en) * | 2020-04-22 | 2021-12-10 | 安徽农业大学 | Method for improving regeneration efficiency in cinnamomum camphora tissue culture process |
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