CN102144493A - Herba epimedii seed propagation method - Google Patents
Herba epimedii seed propagation method Download PDFInfo
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- CN102144493A CN102144493A CN 201110082297 CN201110082297A CN102144493A CN 102144493 A CN102144493 A CN 102144493A CN 201110082297 CN201110082297 CN 201110082297 CN 201110082297 A CN201110082297 A CN 201110082297A CN 102144493 A CN102144493 A CN 102144493A
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Abstract
The invention discloses a herba epimedii seed propagation method which comprises the following steps of: (1) collecting herba epimedii seeds in May and June, and preserving in sand immediately after collection; (2) stirring the seeds preserved in sand with fine soil, and sowing the seeds on an even seed bed; and (3) covering the seeds with fine soil, and preserving moisture by using a covering. Through seed propagation, the propagation coefficient is increased by more than 200 times and is greatly enlarged. Furthermore, by rejuvenating herba epimedii propagated through seeds, the content of general flavone contained in the plants is far higher than that in plants obtained by vegetative propagation.
Description
Technical field
The present invention relates to the breeding field of plant, particularly relate to a kind of barrenwort seed propagation method.
Background technology
Barrenwort is the Berberidaceae barrenwort, is used as medicine to make medicinal material barrenwort usefulness, records in Guizhou Province's medicinal material provincial standard and goes through version, is a dispersed species in Guizhou.Hot, sweet, warm, kidney-replenishing, strengthening the bones and muscles, wind-damp dispelling.In recent years, along with the continuous development of science and technology, the purposes of many renewals constantly is found, and its demand will inevitably be increasing.Guizhou Province with the barrenwort be every year the medical industry gross output value of raw material more than hundred million yuan, but the barrenwort source all is a wild resource, excavates for a long time and has caused the total resources minimizing.Therefore, the expansion population quantity is imperative.
At present, existing seed propagation method is a division propagation, but division propagation coefficient low (1-2), the slow seedling of plant recovers slower behind the division propagation, the resistance of plant strain growth is poor, the growth later stage growing way a little less than, what is more important, long-term vegetative propagation meeting causes the decline of barrenwort quality, and effectively the medicinal ingredient general flavone content can reduce.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of barrenwort seed propagation method.
Barrenwort seed propagation method provided by the invention comprises the steps:
1) gathers the barrenwort seed in 5～June, carry out sand Tibetan after the collection immediately;
2) with after husky seed of hiding of step 1) and the fine earth seed dressing, broadcast sowing on smooth seedbed;
3) after planting cover, cover with covering again and preserve moisture with fine earth.
Seed propagation method provided by the invention, comprise that also seed germination grows cotyledon after, remove the covering except that sawdust, carry out routine servicing and manage.
Wherein, the husky Tibetan time of seed is preferably 4～6 months.
Wherein, carry out sand after described seed and fine sand mix by weight 1: 5～15 and hide, carry out sand after preferably mixing and hide by weight 1: 10.
Wherein, described husky seed and the fine earth mixed dressed seed by weight 1: 5～15, preferably by weight 1: 10.
Wherein, the seeding quantity of mixing husky seed is 60～100g/m
2, be preferably 80g/cm
2
Among the present invention, after planting the thickness that covers with fine earth is 2～3cm.
In one embodiment of the invention, described covering is selected from one or more in liver moss, mulch film, sawdust and the weeds.In a preferred embodiment, cover with sawdust, the thickness of covering is 2cm.In another preferred embodiment, cover with liver moss or hybrid or its combination, cladding thickness is 3～4cm.In another embodiment preferred, cover with mulch film, preferred colourless plastic film, subsides are layered on the seedbed.
Among the present invention, after planting,, in time sprayed in the seedbed, guarantee that the seedbed is moistening according to the humidity of nursery soil.After seed germination grows cotyledon, remove and cover.As if comprising in the covering or being sawdust, then need not remove sawdust.
Just can carry out daily management after sprouting, main management method comprises: water, and weeding and fertilising, concrete operations are carried out according to traditional farming method.
In the prior art, the main modes of reproduction of barrenwort is a division propagation, and reproduction coefficient is 1-2 (promptly 1 maternal plant can only breed the strain of 1-2 stalk).The present invention carries out sand with the barrenwort seed and hides, and has promoted the after-ripening of seed, has improved seed germination rate greatly.The present invention adopts seminal propagation, reproduction coefficient be 400 (be every strain maternal plant about 600 mature seeds of on average collecting seed, about 400 of the seed amount that can be used to sow at last after the treated preservation.) reproduction coefficient improved 200 surplus times, enlarged reproduction coefficient greatly.Further, the general flavone content that is contained with the barrenwort plant of seminal propagation will be higher than the plant that division propagation obtains far away.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 barrenwort seed is husky to be hidden
In the river of cleaning, collect the river sand of cleaning, wash, remove earth, cross the sieve in 50 order apertures.Dry or dry, controlled humidity uses when hiding for seed is husky about 45 °～50 °.
Gather the barrenwort seed in 5～June according to known method, the seed gathered was evenly mixed the In Shade sand Tibetan of carrying out with above-mentioned fine sand by weight 1: 10.The husky Tibetan can be sowed in 4～6 months.
Embodiment 2 seminal propagations
Annual late December dressed seed embodiment 1 husky seed of hiding and fine earth by weight 1: 10, uniform broadcasting is on smooth seedbed, and the seeding quantity of wherein mixing husky seed is 80g/cm
2, after broadcasting, cover 2～3cm fine earth more in the above.
In time cover liver moss, thickness is 3cm.After planting, 40-50 ° of soil moisture of control is in time sprayed, and guarantees that the seedbed is moistening, treats seed germination, grow cotyledon after, remove liver moss.Carry out daily management.The percentage of seedgermination of Sha Zanghou reaches 90%.And the not husky seed of hiding is sowed by above-mentioned same type of seeding, and its percentage of seedgermination is only about 8%.
Embodiment 3
Embodiment 1 husky seed of hiding and fine earth were dressed seed by weight 1: 5, and uniform broadcasting is on smooth seedbed, and the seeding quantity of wherein mixing husky seed is 60g/cm
2, after broadcasting, cover 2～3cm fine earth more in the above.
In time cover colourless plastic film, subsides are layered on the seedbed.After planting, 40-50 ° of soil moisture of control is in time sprayed, and guarantees that the seedbed is moistening, treats seed germination, grow cotyledon after, remove film.Carry out daily management.The percentage of seedgermination of Sha Zanghou reaches 91%.And the not husky seed of hiding is sowed by above-mentioned same type of seeding, and its percentage of seedgermination is only about 9.5%.
Embodiment 4
Embodiment 1 husky seed of hiding and fine earth were dressed seed by weight 1: 15, and uniform broadcasting is on smooth seedbed, and the seeding quantity of wherein mixing husky seed is 100g/cm
2, after broadcasting, cover 2～3cm fine earth more in the above.
In time cover sawdust, cladding thickness is 2cm.After planting, 40-50 ° of soil moisture of control is in time sprayed, and guarantees that the seedbed is moistening, treats seed germination, carries out daily management after growing cotyledon.The percentage of seedgermination of Sha Zanghou reaches 93%.And the not husky seed of hiding is sowed by above-mentioned same type of seeding, and its percentage of seedgermination is only about 10%.
Test example 1 division propagation and seminal propagation plant determination of total flavonoids of the present invention
(1) general flavone assay method
Precision is measured need testing solution (with reference to 2010 editions first one of the Pharmacopoeia of People's Republic of China) 0.5ml under the icariin mensuration item, puts in the 50ml measuring bottle, adds methyl alcohol to scale, shakes up, as need testing solution.It is an amount of that other gets icariin reference substance (purchase of Chinese institute for drug control), and accurate the title decides, and adds the solution that methyl alcohol is made 10 μ g/ml, in contrast product solution.Getting need testing solution and reference substance solution respectively, is blank with the corresponding reagent, according to UV-VIS spectrophotometry (appendix V A), measures absorbance at 270nm wavelength place, calculates, promptly.
2010 editions pharmacopeia regulations are pressed dry product and are calculated, and contain general flavone (TF) with icariin (C
33H
40O
15) meter, must not be less than 5.0%.Table 1 is depicted as by general flavone content in the barrenwort plant of division propagation and seminal propagation relatively, and the result shows that the contained general flavone of the plant of seminal propagation is significantly higher than the division propagation method, and all samples of being surveyed all meets the regulation of pharmacopeia.
General flavone content in the barrenwort plant of table 1 division propagation and seminal propagation relatively
(2) assay method of icariin
(2010 editions pharmacopeia appendix VI D (those skilled in the art know that) measure icariin according to high performance liquid chromatography.
Chromatographic condition and system suitability test
With octadecylsilane chemically bonded silica is filler; Acetonitrile-water (30: 70) is a flowing phase; The detection wavelength is 270nm.Number of theoretical plate calculates by the icariin peak should be not less than 1500.
The reference substance solution preparation
It is an amount of that precision takes by weighing the icariin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.1mg, promptly.
The preparation of need testing solution
Get the about 0.2g of this product blade powder (crossing sieve No. three), the accurate title, decide, and puts in the tool plug conical flask, and the accurate Diluted Alcohol 20ml that adds claims to decide weight, and ultrasonic processing 1 hour claims to decide weight again, supplies the weight that subtracts mistake with Diluted Alcohol, shakes up, and filtration is got subsequent filtrate, promptly.
Determination method
Accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing inject liquid chromatograph, measure, promptly.
2010 editions pharmacopeia regulations, this product blade is pressed dry product and is calculated, and contains (C
33H
40O
15) must not be less than 0.50%.Table 2 is depicted as the comparison by icariin content in the barrenwort plant leaf of division propagation and seminal propagation, the result shows, the contained icariin of the plant of seminal propagation is significantly higher than the division propagation method, and all samples of being surveyed all meets the regulation of pharmacopeia.
Icariin content relatively in the barrenwort blade of table 1 division propagation and seminal propagation
| Sample | The barrenwort source | Division propagation method ICA% | Seed propagation method ICA% |
| 89001 | Peach stockaded village, Leishan | 0.205 | 0.894 |
| 89004 | The Xijiang River, Leishan | 0.010 | 0.570 |
| 0434 | Leishan Lang De | 0.020 | 0.676 |
| 0450 | Danjiang River, Leishan | 0.030 | 1.207 |
| 0453 | The plantation of improving literature | 0.010 | 0.950 |
| 0310 | Longli Wan Zhai | 0.700 | 0.743 |
| 0312 | Longli cat cat head | 0.220 | 0.986 |
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a barrenwort seed propagation method comprises the steps:
1) gathers the barrenwort seed in 5～June, carry out sand Tibetan after the collection immediately;
2) with behind the seed and fine earth seed dressing behind the husky Tibetan of step 1), broadcast sowing on smooth seedbed;
3) after planting cover, cover with covering again and preserve moisture with fine earth.
2. seed propagation method according to claim 1 is characterized in that, comprise that also seed germination grows cotyledon after, the covering except that removing sawdust carries out routine servicing and manages.
3. seed propagation method according to claim 1 and 2 is characterized in that, the husky time of hiding of seed is 4～6 months.
4. seed propagation method according to claim 1 and 2 is characterized in that, carries out sand after seed and fine sand mix by weight 1: 5～15 and hides.
5. seed propagation method according to claim 4 is characterized in that, mixes husky seed and fine earth and dresses seed by weight 1: 5～15.
6. seed propagation method according to claim 1 and 2 is characterized in that, the seeding quantity of mixing husky seed is 60～100g/m
2
7. seed propagation method according to claim 6 is characterized in that, the seeding quantity of mixing husky seed is 80g/m
2
8. seed propagation method according to claim 1 and 2 is characterized in that, after planting the thickness that covers with fine earth is 2～3cm.
9. seed propagation method according to claim 1 and 2 is characterized in that described covering is selected from one or more in liver moss, mulch film, sawdust and the weeds.
10. seed propagation method according to claim 9 is characterized in that, when described covering was liver moss, weeds or its combination, the thickness of covering was 3～4cm; When described covering was sawdust, the thickness of covering was 2cm.
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| CN 201110082297 CN102144493B (en) | 2011-04-01 | 2011-04-01 | Herba epimedii seed propagation method |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145570A (en) * | 2014-08-20 | 2014-11-19 | 宁波市鄞州云帆工程咨询有限公司 | Vision obstruction mode pecking-proof sowing method |
| CN104770099A (en) * | 2015-03-11 | 2015-07-15 | 中国农业大学 | Seedling growing method of Wushan epimedium |
| CN104904467A (en) * | 2015-05-26 | 2015-09-16 | 北京珅奥基医药科技有限公司 | Epimedium planting method |
| CN104904466A (en) * | 2015-05-26 | 2015-09-16 | 北京珅奥基医药科技有限公司 | Planting method for herba epimedii |
| CN104920019A (en) * | 2015-05-26 | 2015-09-23 | 北京珅奥基医药科技有限公司 | Seed planting method of epimediums |
| CN106376345A (en) * | 2016-10-30 | 2017-02-08 | 贵州省蕙安农业科技发展有限公司 | Epimedium dolichostemon Stearn planting method |
| CN107371738A (en) * | 2017-08-24 | 2017-11-24 | 江苏奥沃思特生物科技有限公司 | A kind of implantation methods of Epimedium sagittatum |
| CN109006225A (en) * | 2018-07-17 | 2018-12-18 | 临江市北方道地中药材有限公司 | A kind of implantation methods of Herba Epimedii |
| CN109452098A (en) * | 2018-12-25 | 2019-03-12 | 汉中天然谷生物科技股份有限公司 | A kind of imitating wild planting process of Herba Epimedii |
| CN110876291A (en) * | 2019-11-20 | 2020-03-13 | 陕西金慧方中药科技有限公司 | Method for breaking after-ripening of epimedium seeds |
| CN111149465A (en) * | 2020-02-27 | 2020-05-15 | 通化师范学院 | Method for promoting morphological after-ripening of Korean epimedium seed embryo |
| CN111357574A (en) * | 2018-12-26 | 2020-07-03 | 国药集团同济堂（贵州）制药有限公司 | High-yield planting method of Epimedium sagittatum |
| CN111820083A (en) * | 2019-04-23 | 2020-10-27 | 国药集团同济堂（贵州）制药有限公司 | Seedling growing method of epimedium |
| CN116897776A (en) * | 2023-06-25 | 2023-10-20 | 神农架林区中医药产业协会 | Storage method, culture method and planting method of epimedium seeds |
-
2011
- 2011-04-01 CN CN 201110082297 patent/CN102144493B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| 《中国中药杂志》 20101231 樊家乙等 巫山淫羊藿种子休眠特性及破眠方法研究 3342-3345 1-10 第35卷, 第24期 2 * |
| 《中草药》 20050228 李作洲等 淫羊藿属药用植物的研究现状与展望 289-295 第36卷, 第2期 2 * |
| 《农业与技术》 20061031 刘玉等 长白山区朝鲜淫羊藿林下仿生栽培技术 103-104 1-10 第26卷, 第5期 2 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145570A (en) * | 2014-08-20 | 2014-11-19 | 宁波市鄞州云帆工程咨询有限公司 | Vision obstruction mode pecking-proof sowing method |
| CN104770099A (en) * | 2015-03-11 | 2015-07-15 | 中国农业大学 | Seedling growing method of Wushan epimedium |
| CN104904467A (en) * | 2015-05-26 | 2015-09-16 | 北京珅奥基医药科技有限公司 | Epimedium planting method |
| CN104904466A (en) * | 2015-05-26 | 2015-09-16 | 北京珅奥基医药科技有限公司 | Planting method for herba epimedii |
| CN104920019A (en) * | 2015-05-26 | 2015-09-23 | 北京珅奥基医药科技有限公司 | Seed planting method of epimediums |
| CN106376345A (en) * | 2016-10-30 | 2017-02-08 | 贵州省蕙安农业科技发展有限公司 | Epimedium dolichostemon Stearn planting method |
| CN107371738A (en) * | 2017-08-24 | 2017-11-24 | 江苏奥沃思特生物科技有限公司 | A kind of implantation methods of Epimedium sagittatum |
| CN109006225A (en) * | 2018-07-17 | 2018-12-18 | 临江市北方道地中药材有限公司 | A kind of implantation methods of Herba Epimedii |
| CN109452098A (en) * | 2018-12-25 | 2019-03-12 | 汉中天然谷生物科技股份有限公司 | A kind of imitating wild planting process of Herba Epimedii |
| CN111357574A (en) * | 2018-12-26 | 2020-07-03 | 国药集团同济堂（贵州）制药有限公司 | High-yield planting method of Epimedium sagittatum |
| CN111820083A (en) * | 2019-04-23 | 2020-10-27 | 国药集团同济堂（贵州）制药有限公司 | Seedling growing method of epimedium |
| CN110876291A (en) * | 2019-11-20 | 2020-03-13 | 陕西金慧方中药科技有限公司 | Method for breaking after-ripening of epimedium seeds |
| CN111149465A (en) * | 2020-02-27 | 2020-05-15 | 通化师范学院 | Method for promoting morphological after-ripening of Korean epimedium seed embryo |
| CN111149465B (en) * | 2020-02-27 | 2023-08-04 | 通化师范学院 | Method for promoting morphological after-ripening of Korean epimedium seed embryo |
| CN116897776A (en) * | 2023-06-25 | 2023-10-20 | 神农架林区中医药产业协会 | Storage method, culture method and planting method of epimedium seeds |
| CN116897776B (en) * | 2023-06-25 | 2024-03-29 | 神农架林区中医药产业协会 | Storage method, culture method and planting method of epimedium seeds |
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Inventor after: Zhou Ning Inventor after: Guo Baolin Inventor after: He Yong Inventor after: Yang Xiangbo Inventor after: Li Li Inventor after: Wang Xincun Inventor after: Chen Debin Inventor after: Feng Zexi Inventor after: Zeng Xianglan Inventor before: Zhou Ning Inventor before: He Yong Inventor before: Yang Xiangbo Inventor before: Li Li Inventor before: Wang Xincun Inventor before: Chen Debin Inventor before: Feng Zexi Inventor before: Zeng Xianglan |
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