Summary of the invention
At the deficiencies in the prior art, the objective of the invention is to design the technical scheme that a kind of health food and uses thereof is provided, adopt the broken down extraction raw material of low temperature, produce in conjunction with the low-temperature operation technology of flash concentration, the functional component purity height of the health products that make, quality better, this health food health nutrient is worth height, result of treatment is remarkable, and production technology is simple.
Described a kind of health food, it is characterized in that forming: Abelmoschus Esculentus Linn extract 20~50%, the real extract 10~30% of bowl hesperidium aurantium, sophora bud extract 10~20%, Honegsukle flower P.E 5~20%, green-tea extract 5~20%, dendrobium candidum powder 5~20%, American Ginseng powder 5~20% by the following material of weight percentage;
Described Abelmoschus Esculentus Linn extract adopts the preparation of following method: the tender fruit of okra is dried after with the section of crosspiece face or air-dry, the okra fruit of drying is placed in the container of broken extractor, add 30~60% hydrous ethanols or water, starting switch, rotating speed with 10000 r/min carries out the broken extraction of room temperature texture 2~3 times, raw material is broken into 40 purpose granularities, if okra fruit dry weight is that then hydrous ethanol or water consumption are 6~10 liters to 1kg, extraction time is 1~3 minute, with the extract suction filtration or get rid of filter, discard filter residue, the extract that obtains is at 50 ℃~80 ℃, vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulates the feed liquor speed of solution to be concentrated, with 200~300 mL min
-1Flow be concentrated into 1/5~1/8 of original volume repeatedly, concentrate is carried out vacuum drying becomes dry powder, obtain okra fruit extract dry powder with the mortar porphyrize after by 100 mesh sieves;
The real extract of described bowl hesperidium aurantium adopts following method preparation: the real peeling of bowl hesperidium aurantium is placed in the container of broken extractor, starting switch, carrying out high-speed stirred with the rotating speed of 10000 r/min smashed to pieces 2~5 minutes, raw material is broken into 40 purpose granularities, fruit after the fragmentation is migrated out, push or get rid of filter after packing tightly with coarse cloth, discard pomace, the extract that obtains is at 60 ℃~80 ℃, vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulate the feed liquor speed of solution to be concentrated, with 200~300 mL min
-1Flow to be concentrated into thickness repeatedly aqueous, viscous fluid is carried out vacuum drying below 60 ℃ becomes dry powder, obtain the real extract dry powder of bowl hesperidium aurantium with the mortar porphyrize after by 100 mesh sieves;
Described sophora bud extract adopts following method preparation: the bud of japanese pagoda tree of drying is put in the container of broken extractor, the warm water that adds 60 ℃~80 ℃, starting switch, rotating speed with 10000 r/min carries out historrhexis's extraction 2~3 times, raw material is broken into 40 purpose granularities, if sophora bud dry weight be 1kg then the warm water consumption be 6~12 liters, each extraction time is 1~3 minute, with the extract suction filtration or get rid of filter, discard filter residue, the extract that merging obtains is at 50 ℃~80 ℃, and vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulate the feed liquor speed of solution to be concentrated, with 200~300 mL min
-1Flow be concentrated into repeatedly 1/2~1/3, concentrate is placed into room temperature, separate out a large amount of yellow mercury oxides, suction filtration carries out vacuum drying with the sediment that obtains behind the suction filtration, obtains sophora bud extract dry powder with the mortar porphyrize after by 100 mesh sieves;
Described Honegsukle flower P.E adopts following method preparation: the honeysuckle of drying is put in the container of broken extractor, add 50~80% hydrous ethanols, starting switch, rotating speed with 10000 r/min carries out the broken extraction of room temperature texture 1~3 time, raw material is broken into 40 purpose granularities, if honeysuckle dry weight be 1kg then the hydrous ethanol consumption be 6~12 liters, each extraction time is 2~5 minutes, with the extract suction filtration, discard filter residue, the extract that merging obtains is at 50 ℃~70 ℃, and vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulate the feed liquor speed of solution to be concentrated, with 200~300 mL min
-1Flow be concentrated into repeatedly 1/5~1/10, concentrate is carried out vacuum drying becomes dry powder, obtain Honegsukle flower P.E dry powder with the mortar porphyrize after by 100 mesh sieves;
Described green-tea extract adopts following method preparation: the green tea of drying is put in the container of broken extractor, add 50~70% hydrous ethanols or aqueous acetone, starting switch, rotating speed with 10000 r/min carries out the broken extraction of room temperature texture 1~2 time, raw material is broken into 40 purpose granularities, if green tea dry weight is that then hydrous ethanol or aqueous acetone consumption are 6~10 liters to 1kg, each extraction time is 2~3 minutes, with the extract suction filtration, discard filter residue, the extract that merging obtains is at 50 ℃~70 ℃, vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulate the feed liquor speed of solution to be concentrated, with 200~300 mL min
-1Flow be concentrated into repeatedly 1/5~1/8, then concentrate is proceeded vacuum drying and becomes dry powder, obtain green-tea extract dry powder with the mortar porphyrize after by 100 mesh sieves.
Described a kind of health food, the content that it is characterized in that described Abelmoschus Esculentus Linn extract is 25~45%, preferred 30~40%.
Described a kind of health food is characterized in that the content of the real extract of described bowl hesperidium aurantium is 15~25%, preferred 18~20%.
Described a kind of health food, the content that it is characterized in that institute's sophora bud extract is 12~18%, preferred 15~17%.
Described a kind of health food, the content that it is characterized in that institute's Honegsukle flower P.E is 8~18%, preferred 10~15%.
Described a kind of health food, the content that it is characterized in that institute's green-tea extract is 8~18%, preferred 10~15%.
Described a kind of health food, the content that it is characterized in that institute's dendrobium candidum powder is 8~18%, preferred 10~15%.
Described a kind of health food, the content that it is characterized in that institute's American Ginseng powder is 8~18%, preferred 10~15%.
Described a kind of health food is characterized in that among the preparation method of described Abelmoschus Esculentus Linn extract: the concentration of hydrous ethanol is 40~55%, and is preferred 45%~50%, and during the extract flash concentration, feed liquor speed is 220~280 mLmin
-1, preferred 250~260 mLmin
-1, temperature is 60 ℃~75 ℃, preferred 65 ℃~70 ℃;
In the real preparation method of extract of described bowl hesperidium aurantium: during the extract flash concentration, feed liquor speed is 220~280 mLmin
-1, preferred 250~260 mLmin
-1Temperature is 65 ℃~75 ℃, preferred 70 ℃~72 ℃;
In the described sophora bud preparation method of extract: warm water temperature is 65 ℃~75 ℃, preferred 70 ℃~72 ℃; During the extract flash concentration, feed liquor speed is 220~280 mLmin
-1, preferred 250~260 mLmin
-1Temperature is 60 ℃~70 ℃, preferred 65 ℃~68 ℃;
Among the preparation method of described Honegsukle flower P.E: the concentration of hydrous ethanol is 55~75%, and is preferred 60~70%, and during the extract flash concentration, feed liquor speed is 220~280 mLmin
-1, preferred 250~260 mLmin
-1Temperature is 60 ℃~70 ℃, preferred 65 ℃~68 ℃;
Among the preparation method of described green-tea extract: the concentration of hydrous ethanol or aqueous acetone is 55~65%, and is preferred 60~63%, and during the extract flash concentration, feed liquor speed is 220~280 mLmin
-1, preferred 250~260 mLmin
-1Temperature is 55 ℃~65 ℃, preferred 60 ℃~65 ℃.
The application of described a kind of health food in antibacterial, anti-oxidant, the antifatigue of preparation, hypoglycemic product.
Described broken extractor can directly be buied from the market, and Reduced Pressure Concentration Device (flash concentration device) is design and assembly voluntarily, also can directly buy from the market.
Above-mentioned plant extracts all adopts broken extraction and flash concentration special process to be processed into, and compared with prior art, the present invention has following advantage:
1. among the present invention okra fruit, bowl hesperidium aurantium reality, the sophora bud, honeysuckle, green tea are all adopted broken the extraction, this process is that fragmentation and leaching process are united two into one.Cardinal principle is at room temperature, materials such as the different parts of plant and appropriate solvent are put in the extractor, utilize the cutter of rotation at a high speed that the plant fragmentation is trimmed to fine particle, simultaneously by realizing the combination of high-speed stirred, vibration, mixing, infiltration, dipping, the active ingredient that makes medicinal material inside is transferred in the solvent in the extremely short time and reaches balance, realizes extracting purpose by filtering.Finish when this extracting method has realized pulverizing and leaching process, the broken extraction once only needs 1~3 min, and leaching process need not heating simultaneously, the possibility of avoiding thermo-labile component to destroy.Show that by experiment this extracting method is widely applicable, be applicable to the extraction of the medicinal material and the multiple solvent of various character, have extract quick, complete, energy-efficient, easy and simple to handle, safeguard and clean convenient, environmental, operating efficiency than advantages such as height.Adopting this method to extract can extract the most of functional component in the above natural plants fully, has improved functional component recovery rate and content.
2. adopt Reduced Pressure Concentration Device to carry out flash concentration to okra fruit, bowl hesperidium aurantium reality, the sophora bud, honeysuckle, green tea extractive liquor among the present invention.This device is a kind of concentrated solution rapidly and efficiently or the flash distillation plant that reclaims solvent, basic principle is under reduced pressure, liquid to be concentrated is at the uniform velocity entered in the thin film evaporation pipe with thread, and the back formation film that is heated in pipe makes the partial solvent gasification and reaches concentrated purpose.Use this device to concentrate and have following advantage: it is low to carry out the flash concentration heating temperature, compole short (several seconds) when being concentrated heated liquid, and the concentrate temperature of recovery is lower, effectively protects the thermal sensitivity composition not to be destroyed, the solvent recovering yield height.This installed capacity is big, is not concentrated the restriction of the long-pending size of liquid, and it is concentrated to circulate continuously, volume required up to being concentrated into.The concentrated speed of this device is fast, efficient is high, simple to operate, easy to use, energy-conserving and environment-protective.This extraction concentration technique can avoid directly with water boiling and extraction and water extract need under higher temperature, concentrate for a long time may bring the influence to functional component.
Described a kind of health food, make by multiple natural plant extracts and rare Chinese medicine combination, the synergy that can effectively realize acting on a plurality of target organs and bring into play, has antifatigue, strengthen human tolerance and immunity of organisms, antibacterial, anti-inflammatory, clearing heat and detoxicating, relieve inflammation or internal heat and make eye bright, liver protecting, the strong kidney of establishing-Yang, anti-oxidant, anti-ageing, remove free radical, hypotensive, hypoglycemic, the effect of aspects such as aid digestion and stomach-invigorating intestine-moisturizing, its health nutrient is worth high, result of treatment is remarkable, takes for a long time not only to have the good health care effect, also has good therapeutic action.And reasonable recipe, easy to use, production technology is simple, can realize suitability for industrialized production, has improved the added value of multiple natural plants and the utilization rate of resource.
The percentage composition that relates in the present specification unless otherwise indicated all refers to weight percentage.
The specific embodiment
Now in conjunction with embodiments of the invention and correlation test, the invention will be further described.
Embodiment 1: this health food is formed according to following weight proportion: Abelmoschus Esculentus Linn extract 50%, the real extract 10% of bowl hesperidium aurantium, sophora bud extract 10%, Honegsukle flower P.E 10%, green-tea extract 10%, dendrobium candidum powder 5%, American Ginseng powder 5%.
Embodiment 2: this health food is formed according to following weight proportion: Abelmoschus Esculentus Linn extract: 40%, the real extract 20% of bowl hesperidium aurantium, sophora bud extract 10%, Honegsukle flower P.E 10%, green-tea extract 10%, dendrobium candidum powder 5%, American Ginseng powder 5%.
Embodiment 3: this health food is formed according to following weight proportion: Abelmoschus Esculentus Linn extract: 30%, the real extract 20% of bowl hesperidium aurantium, sophora bud extract 10%, Honegsukle flower P.E 10%, green-tea extract 10%, dendrobium candidum powder 10%, American Ginseng powder 10%.
Embodiment 4: this health food is formed according to following weight proportion: Abelmoschus Esculentus Linn extract: 30%, the real extract 20% of bowl hesperidium aurantium, sophora bud extract 20%, Honegsukle flower P.E 10%, green-tea extract 10%, dendrobium candidum powder 5%, American Ginseng powder 5%.
Embodiment 5: this health food is formed according to following weight proportion: Abelmoschus Esculentus Linn extract: 20%, the real extract 20% of bowl hesperidium aurantium, sophora bud extract 20%, Honegsukle flower P.E 10%, green-tea extract 10%, dendrobium candidum powder 10%, American Ginseng powder 10%.
Embodiment 6: this health food is formed according to following weight proportion: Abelmoschus Esculentus Linn extract: 40%, the real extract 20% of bowl hesperidium aurantium, sophora bud extract 20%, Honegsukle flower P.E 5%, green-tea extract 5%, dendrobium candidum powder 5%, American Ginseng powder 5%.
Can be made into formulations such as lozenge, capsule, granule after above-mentioned prescription mixed according to above weight ratio.The preparation of corresponding buccal tablet needs to add auxiliary materials such as an amount of Icing Sugar, cornstarch, sodium carboxymethylcellulose, dolomol, ethanol as excipient, the processing by auxiliary material, pulverize, sieve, mix, operations such as granulation, compressing tablet, drying finish; The preparation of corresponding granule formulation needs to add auxiliary materials such as a spot of Icing Sugar and dextrin as excipient, adds a spot of wetting agent again, and operations such as the processing by auxiliary material, granulation, drying, whole grain are finished.The preparation of corresponding capsule preparations needs to add a spot of starch, adopts conventional method that the dry powder and the auxiliary material of above-mentioned prescription evenly are mixed and made into capsule.
Described Abelmoschus Esculentus Linn extract adopts the preparation of following method: the tender fruit of okra is dried after with the section of crosspiece face or air-dry, the okra fruit of drying is placed in the container of broken extractor, add 50% hydrous ethanol or water, starting switch, rotating speed with 10000 r/min carries out the broken extraction of room temperature texture 2 times, and raw material is broken into 40 purpose granularities.If okra fruit dry weight is that then hydrous ethanol or water consumption are 8 liters to 1kg, extraction time is 2 minutes, with the extract suction filtration or get rid of filter, discard filter residue, the extract that obtains is at 80 ℃, vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulates the feed liquor speed of solution to be concentrated, with 230 mLmin
-1Flow be concentrated into 1/7 of original volume repeatedly, concentrate is carried out vacuum drying becomes dry powder, with obtaining okra fruit extract dry powder behind the mortar porphyrize.In the Abelmoschus Esculentus Linn extract preparation method: the concentration of hydrous ethanol is respectively 30%, 40%, 45%, 60%, broken extraction time is 3 times, 3 times, 1 time, 2 times, the hydrous ethanol consumption is 10 liters, 9 liters, 6 liters, 8 liters, and broken extraction time is 3 minutes, 3 minutes, 1 minute, 2 minutes; The feed liquor speed of concentrate is 200 mLmin
-1, 250 mLmin
-1, 280 mLmin
-1, 300 mLmin
-1, the flash concentration temperature is 50 ℃, 60 ℃, 65 ℃, 70 ℃, extract is concentrated into 1/8,1/7,1/5,1/6 of original volume, also can obtain described beneficial effect.Okra fruit extract yield reaches 22.8%~42.6% after measured, and content of total flavone reaches 6.4%~18.8% in the okra fruit extract.
The real extract of described bowl hesperidium aurantium adopts following method preparation: the real peeling of bowl hesperidium aurantium is placed in the container of broken extractor, starting switch, carrying out high-speed stirred with the rotating speed of 10000 r/min smashed to pieces 2 minutes, fruit after the fragmentation is migrated out, push or get rid of filter after packing tightly with coarse cloth, discard pomace, the extract that obtains is at 60 ℃, vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulates the feed liquor speed of solution to be concentrated, with 240 mLmin
-1Flow be concentrated into to thickness aqueously repeatedly, viscous fluid is carried out vacuum drying below 60 ℃ becomes dry powder, with obtaining the real extract dry powder of bowl hesperidium aurantium behind the mortar porphyrize.In the preparation method of bowl hesperidium aurantium bark extract: the high-speed stirred time of smashing to pieces was respectively 3 minutes, 4 minutes, 5 minutes, 4 minutes, and the feed liquor speed of concentrate is 200 mLmin
-1, 230 mLmin
-1, 260 mLmin
-1, 280 mLmin
-1, extract flash concentration temperature is 65 ℃, 70 ℃, 75 ℃, 75 ℃, also can obtain described beneficial effect.Content of total flavone reaches 5.5%~16.8% in the real extract dry powder of bowl hesperidium aurantium after measured.
Described sophora bud extract adopts the preparation of following method: the bud of japanese pagoda tree of drying is put in the container of broken extractor, is added 60 ℃ warm water, starting switch carries out historrhexis's extraction 2 times with the rotating speed of 10000 r/min, and raw material is broken into 40 purpose granularities.If sophora bud dry weight be 1kg then the warm water consumption be 12 liters, each extraction time is 3 minutes, with the extract suction filtration or get rid of filter, discard filter residue, the extract that merging obtains is at 80 ℃, vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulates the feed liquor speed of solution to be concentrated, with 260 mLmin
-1Flow be concentrated into 1/3 repeatedly, concentrate is placed into room temperature, separate out a large amount of yellow mercury oxides, suction filtration carries out vacuum drying with the sediment that obtains behind the suction filtration, with obtaining sophora bud extract dry powder behind the mortar porphyrize.In sophora bud preparation method of extract: the temperature of warm water is respectively: 70 ℃, 80 ℃, broken extraction time is 1 time, 3 times, the hydrous ethanol consumption is 10 liters, 6 liters, 9 liters, 8 liters, and broken extraction time is 2 minutes, 1 minute, and the feed liquor speed of concentrate is 200 mLmin
-1, 230 mLmin
-1, 280 mLmin
-1, 300 mLmin
-1, the flash concentration temperature is 50 ℃, 60 ℃, 70 ℃, extract is concentrated into 1/2 of original volume, also can obtain described beneficial effect.Sophora bud extract dry powder yield reaches 23.4%~38.8% after measured, and the content of general flavone in the sophora bud extract (rutin) reaches 78.5%~95.7%.
Described Honegsukle flower P.E adopts following method preparation: the honeysuckle of drying is put in the container of broken extractor, add 50% hydrous ethanol and carry out the broken extraction of room temperature texture 1 time, if honeysuckle dry weight be 1kg then the hydrous ethanol consumption be 6 liters, each extraction time is 5 minutes, with the extract suction filtration, discard filter residue, merging the extract that obtains adopts Reduced Pressure Concentration Device to carry out flash concentration to 1/10 of original volume at 60 ℃, concentrate is carried out vacuum drying become dry powder, with obtaining Honegsukle flower P.E dry powder behind the mortar porphyrize.In the preparation method of Honegsukle flower P.E: the concentration of hydrous ethanol is respectively 60%, 70%, 80%, broken extraction time is 2 times, 3 times, the hydrous ethanol consumption corresponds to 6 liters, 8 liters, 9 liters, 10 liters, broken extraction time is 2 minutes, 4 minutes, 5 minutes, the flash concentration temperature is 50 ℃, 70 ℃, extract is concentrated into 1/8,1/7,1/6,1/9 of original volume, also can obtain described beneficial effect.Reach 18.86%~30.4% through the Honegsukle flower P.E yield, chlorogenic acid contents reaches 1.6%~4.8% in the Honegsukle flower P.E.
Described green-tea extract adopts following method preparation: the green tea of drying is put in the container of broken extractor, starting switch, add 50% hydrous ethanol or aqueous acetone with the rotating speed of 10000 r/min and carry out that room temperature texture is broken to extract 2 times, raw material is broken into 40 purpose granularities.If green tea dry weight is that then hydrous ethanol or aqueous acetone consumption are 8 liters to 1kg, each extraction time is 3 minutes, with the extract suction filtration, discard filter residue, the extract that merging obtains is at 60 ℃, vacuum is to adopt Reduced Pressure Concentration Device to carry out flash concentration under the 0.095 Mpa condition, regulates the feed liquor speed of solution to be concentrated, with 220 mLmin
-1Flow be concentrated into 1/6 repeatedly, then concentrate is proceeded vacuum drying and becomes dry powder, with obtaining green-tea extract dry powder behind the mortar porphyrize.In the preparation method of green-tea extract: the concentration of hydrous ethanol or aqueous acetone is respectively 60%, 70%, broken extraction time is 1 time, the hydrous ethanol consumption corresponds to 6 liters, 7 liters, 9 liters, 10 liters, broken extraction time is 2 minutes, 4 minutes, 5 minutes, and the feed liquor speed of concentrate is 200 mLmin
-1, 250 mLmin
-1, 280 mLmin
-1, 300 mLmin
-1, the flash concentration temperature is 50 ℃, 70 ℃, extract is concentrated into 1/5,1/7,1/8,1/9 of original volume, also can obtain described beneficial effect.The green-tea extract yield reaches 32.2%~44.6% after measured, and the content of Tea Polyphenols reaches 23.6%~30.4% in the green-tea extract.
Described dendrobium candidum powder adopts superfine communication technique that pulverizing medicinal materials is become superfine powder with American Ginseng powder, can be by the above sub-sieve of 200 orders.
For further specifying health-care efficacy of the present invention,, antibacterial activity measurement result anti-oxidant below by carrying out and antifatigue and hypoglycemic test describe.
1. bacteriostatic activity test
The mensuration of bacteriostatic activity adopts agar diffusion filter paper method, judges bacteriostasis according to inhibition zone diameter.Filter paper diameter 6.0 mm, standby behind the sterilizing-drying.The data SPSS1010 analyzes, and relatively adopts between group
tCheck.
1) for the preparation of test agent solution: take by weighing the health food of a certain amount of embodiment 1 preparation respectively, parallelly take by weighing 3 parts, adding distil water is settled in the volumetric flask, and dilution is solution in different concentration successively then, and does blank with sterilized distilled water.
2) mensuration of bacteriostatic experiment and inhibition zone: add in the sterile petri dish with the various bacteria suspensions of aseptic pipette, extract, soak into the filter paper of sample solution, place it in media surface with the tweezers gripping.The bacterium culture dish that contains of putting filter paper well is cultivated in insulating box respectively, then taken out, measure and write down the diameter of the antibacterial ring that forms.Every group of experiment repeats 3 times, and experimental result is averaged.
3) mensuration of minimal inhibitory concentration (MIC) measurement result: MIC adopts doubling dilution.The sample solution of variable concentrations is moved in each plate with pipette respectively, pour in the culture medium of high-temperature sterilization fully mixing into, after the cooled and solidified, add bacteria suspension in every ware, be coated with aseptic spreader and evenly cultivate.Take out, observed result, with the solution concentration of long bacterium not as minimum inhibitory concentration MIC, the result for test agent to the bacteriostatic test of staphylococcus aureus as a result MIC be 38.5 μ gmL
-1, to colibacillary bacteriostatic test as a result MIC be 55.7 μ gmL
-1This result of the test shows, supplies the aqueous solution of test agent all to have stronger inhibitory action to supplying examination bacterial classification staphylococcus aureus, Escherichia coli, and strengthens with the increase fungistatic effect of concentration.Under the same concentrations condition for test agent to aureus with inhibition obviously greater than Escherichia coli.This result of the test shows that the health food of embodiment 1 preparation has stronger bacteriostatic activity, carries out same test with embodiment 2-6, also can reach essentially identical beneficial effect.
2. antioxidation activity test:
1) instrument and reagent
Infinite M 200 ELIASAs (Switzerland Tecan); UV-2102 PCS ultraviolet-uisible spectrophotometer (Shanghai You Nike Instr Ltd.); 1, the bitter diazanyl free radical (DPPH, sigma company) of 1-diphenyl-2-.
2) preparation of confession test agent solution
Accurately take by weighing a certain amount of health food respectively, in 100 ml measuring bottles, with 60 % ethanol constant volumes, ultrasonic dissolution by embodiment 1 preparation.It is stand-by to place 4 ℃ of refrigerators to preserve for test agent solution.
3) DPPH radical scavenging activity measuring principle
The DPPH method is one of common method of radical scavenging activity detection, can be used for the evaluation of various natural plant extracts antioxidation activities.DPPH is a kind of stable free radical in organic solvent, at 5l7 nm strong absorption is arranged.When having free radical scavenger, owing to the pairing of scavenger and DPPH single electron reduces its absorption, the variation of its absorbance becomes quantitative relationship with its electron number of accepting, and available AAS carries out quantitative analysis.Make positive control with Trolox in the sample determination process, and converse the total oxidation resistance of sample with this, measurement result is expressed as and reaches the needed Trolox concentration of the suitable oxidation resistance of finite concentration test substances.Claim that this method is the TEAC method.The TEAC value representation is the content that sample is equivalent to Trolox when reaching the half clearance rate.The TEAC value is big more, shows that removing free radical ability is strong more.IC
50Value is a parameter that is usually used in estimating oxidation resistance, required concentration when it is meant the DPPH free radical of antioxidant for clearing 50%.Its value is more little, and when expression reached 50% clearance rate, the concentration dose of used free radical scavenger was more little, and its removing effect to free radical is also just good more, and the corresponding sample antioxidation activity of participating in the experiment is strong more.
4) assay method accurately takes by weighing a certain amount of DPPH reagent, with the dissolving of 95% ethanol, and quantitatively changes in the 100 mL measuring bottles, with 95 % ethanol constant volumes, shakes up to such an extent that mass concentration is the DPPH stock solution of 59.0 μ g/mL, and it is standby to place refrigerator and cooled to hide.The sample solution 200 μ L and the 59.0 μ g/mL DPPH test solution 100 μ L that in 96 hole ELISA Plates, add variable concentrations respectively.Sample adds back concussion 30 s, behind 24 ℃ of insulation 30 min, measures its light absorption value (Ap) under 517 nm wavelength, measures simultaneously not add the sample blank light absorption value (Ac) of DPPH and add DPPH but do not add the light absorption value (Amax) of sample.With each concentration (μ g/mL) for test agent is abscissa, is that ordinate is drawn directrix curve with the free radical scavenging activity (Y) that records, according to regression equation calculation IC
50Making positive control with Trolox, is abscissa with Trolox (X) concentration, is that ordinate is made calibration curve with free radical scavenging activity (Y), obtains regression equation, according to regression equation calculation IC
50, free radical scavenging activity=1-(Ap-Ac)/Amax * 100%.
Result of calculation Trolox is to the IC of DPPH clearance rate
50Value is 19.27 μ g/mL, for the IC of test agent to the DPPH clearance rate
50Value is 41.26 μ g/mL.Show that for the consumption of test agent when reaching the half clearance rate be 2.1 times of Trolox consumption.The result shows for test agent the DPPH free radical to be had certain clearance rate, and clearance rate improves with the increase for the test agent solution concentration.The health food that shows embodiment 1 preparation has stronger antioxidation activity, carries out same test with embodiment 2-6, also can reach essentially identical beneficial effect.
3. antifatigue and hypoglycemic test
By human feeding trial, select the volunteer meet experimental condition to take the capsule preparations of the present invention's preparation, result of the test sees Table 1 and table 2.
1 pair of experimenter group's anti-fatigue test of table 1 embodiment is table as a result
Experimenter's number |
Experimenter group's symptom |
Observing time |
Take the back recovery situation |
Total effective rate |
30 people |
The body void crowd that suffers from a deficiency of the kidney, body weight is more fat.Usually dizzy at ordinary times, ache all over, lassitude hypodynamia, Low Back Pain, cold perspiration, often insomnia, decrease of memory, minor illness is constant, normal cat fever. |
45 days |
Physical and energetic, have rosy cheeks, the energy foot, passive protective physical fitness obviously improves, and is seldom sick. |
56%~84% |
The hypoglycemic result of the test table of 1 couple of experimenter group of table 2 embodiment
Experimenter's number |
Experimenter group |
Requirement during taking |
Observing time |
Take back blood sugar decline percentage |
Total effective rate |
30 people |
Hyperglycemia population, subject age, the course of disease, blood sugar level and medicining condition no significant difference |
Adhere to diet control, it is constant to take hypoglycemic medicament categories and dose originally |
45 days |
Measure blood glucose value on an empty stomach after decline 6.4%~8.43(test-meal) |
56%~84% |
With embodiment 2-6 the general population is carried out same test, also can reach essentially identical beneficial effect.