CN102132789B - Method for preparing stichopus japonicus feed by using hydrolyzed fish meal and fermented bean pulp as main protein source - Google Patents
Method for preparing stichopus japonicus feed by using hydrolyzed fish meal and fermented bean pulp as main protein source Download PDFInfo
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- CN102132789B CN102132789B CN2011100798643A CN201110079864A CN102132789B CN 102132789 B CN102132789 B CN 102132789B CN 2011100798643 A CN2011100798643 A CN 2011100798643A CN 201110079864 A CN201110079864 A CN 201110079864A CN 102132789 B CN102132789 B CN 102132789B
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- fish meal
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- stichopus japonicus
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- fermented bean
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a method for preparing stichopus japonicus feed by using hydrolyzed fish meal and fermented bean pulp as a main protein source, which comprises the following steps: grinding fish meal and bean pulp, which serve as the raw materials, to 200 meshes; soaking the fish meal in deionized water, regulating the pH value to 2.0 with hydrochloric acid, adding pepsase and trypsase, hydrolyzing fish meal for 20 hours at 37 DEG C, collecting hydrolysis solution, absorbing and drying to obtain hydrolyzed fish meal; sterilizing the bean pulp, placing the bean pulp into a fermentation tank, inoculating activated bacillus and lactobacillus, and fermenting at an initial temperature of 40 DEG C and pH value of 7 for 48 hours to obtain a fermented product; and mixing the hydrolyzed fish meal and the fermented bean pump to form the protein source of the feed, and mixing the protein source with an energy feed to obtain the stichopus japonicus feed. The method has the advantages of advanced process, high digestibility and capability of promoting growth. The feed prepared by the method can improve the immunity and disease resistance in stichopus japonicas and improve the culture survival rate of the stichopus japonicas.
Description
Technical field
The present invention relates to a kind of compound method of holothurian feed, particularly a kind of is the method for major protein source preparation stichopus japonicus feed with hydrolysis fish meal and fermented bean dregs.
Background technology
Because the stichopus japonicus digestive system is simple in structure, the digestive ferment hyposecretion, to the ability of nutrient digestion a little less than, existing stichopus japonicus feed is in default of the pretreating process of raw material, digestibility was on the low side after stichopus japonicus ingested, over-burden in the digestion of feed, not only influenced the growth of stichopus japonicus, also reduced the utilization rate of feed.
Summary of the invention
The compound method that the purpose of this invention is to provide a kind of high-quality and efficient stichopus japonicus feed, the stichopus japonicus feed of this method preparation are conducive to improve digestibility, accelerate the stichopus japonicus speed of growth, promote immunity, and the efficiency of feed utilization height overcomes the deficiency that has the stichopus japonicus feed now.
Of the present invention is the method for major protein source preparation stichopus japonicus feed with hydrolysis fish meal and fermented bean dregs, and step is as follows:
A, be basic material and carry out pulverization process with fish meal and soybean meal, pulverize the back granularity and reach 200 orders;
B, fish meal is soaked with deionized water, the volume ratio of fish meal and deionized water is 1:2, soaking temperature is 25 ℃, and be 2.0 with the pH that hydrochloric acid is regulated fish meal and deionized water mixture, add pepsin and trypsase then, pepsin and tryptic amount ratio are 10: 1, pepsin and trypsase are 1:2500 with the mass ratio of fish meal and deionized water mixture, then, under 37 ℃ condition, fish meal carried out 20 hours constant temperature hydrolysis, collect hydrolyzate through after the centrifugal impurity elimination with namely making hydrolysis fish meal product after organic carrier absorption, the drying; Described pepsic vigor is at least 4000IU/g, and tryptic vigor is at least 2500IU/mg;
C, soybean meal is carried out putting into fermentation tank after the sterilization treatment, in fermentation tank, inoculate bacillus and the Bacillus acidi lactici that passes through activation culture then, the mass ratio of soybean meal and bacillus and Bacillus acidi lactici is 100:1:2, control fermentation initial temperature is 40 ℃, material is 1: 1 with the volume ratio of water, initial pH value is 7, obtains fermented product through 48 hours ferment at constant temperature, with fermented product after through 60 ℃ of constant temperature drying moisture fermented bean dregs; The concentration of described bacillus is at least 1 * 10
9Individual/g, the concentration of Bacillus acidi lactici is at least 5 * 10
8Individual/g;
D, the hydrolysis fish meal is mixed the protein sources that forms feed with fermented bean dregs, the quality proportioning of hydrolysis fish meal and fermented bean dregs is 1:1.5, protein sources is mixed forming the stichopus japonicus feed with energy feed, and the mass ratio of protein sources and energy feed is 1:3.
Described energy feed is wheaten starch and seawood meal and ooze, and the quality proportioning of wheaten starch and seawood meal and ooze is 3: 3: 4.
Of the present invention is that the method for major protein source preparation stichopus japonicus feed has the technology advanced person, is easy to digest, promotes the advantage of growing with hydrolysis fish meal and fermented bean dregs.
It is simple in structure to the present invention is directed to stichopus japonicus digestive system physiological tissue, the digestive ferment hyposecretion, the biology characteristics that the nutrient digestion ability is weak, utilize protein raw material through the technology of extracorporeal hydrolysis and fermentation, alleviate the stichopus japonicus digestive system to the digestion burden of feedstuff, save stichopus japonicus digestion energy consumption, promote the utilization ratio of feed greatly.Simultaneously, utilize the rapid breeding amplification of beneficial bacterium in the bean pulp fermentation process, a large amount of beneficial bacterium live bodies is introduced in the stichopus japonicus body by the feed approach, effectively strengthen the immune disease-resistance ability of stichopus japonicus, promote the apostichopus japonicus culture survival rate.
Use the culture experiment of the stichopus japonicus feedstuff feeding stichopus japonicus of the present invention's preparation to show, the immune disease-resistance power index that the experimental group stichopus japonicus shows significant growth fast and obviously raises than control group.Specific targets are: stichopus japonicus children ginseng (average specification 50-100g/ head), under 15 ℃ of conditions of temperature of cultivation, through 3 months breed contrast test, the absolute growth speed of experimental group stichopus japonicus and relative growth rate improved 16.7% and 19.6% than control group respectively.After culture experiment finishes, the immune relevant enzyme index of determination experiment group stichopus japonicus and control group stichopus japonicus shows, the vigor index of acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LSZ), superoxide dismutase four kinds of important immune enzymes such as (SOD) all has raising in various degree, wherein the acid phosphatase of experimental group (ACP), alkaline phosphatase (AKP), lysozyme (LSZ) vigor improve 12.3%, 17.7% and 28.1% respectively than control group, and experimental group stichopus japonicus superoxide dismutase (SOD) vigor is not remarkable than the control group increase.Pond scale test also shows, the efficient stichopus japonicus compound feed that this invention of throwing something and feeding is produced, and the culture-cycle of stichopus japonicus will on average shorten 3 months, and the average annual output of apostichopus japonicus culture obviously improves.
The specific embodiment
Of the present invention is the method for major protein source preparation stichopus japonicus feed with hydrolysis fish meal and fermented bean dregs, and its step is as follows:
A, employing Peru produce high-quality fish meal and Heilungkiang product high quality soybean dregs of beans is basic material and carries out pulverization process respectively that granularity is 200 orders after pulverizing;
B, the fish meal after will pulverizing soak with deionized water, and the volume ratio of fish meal and deionized water is 1:2; Soaking temperature is controlled to be 25 ℃; And be 2.0 with the pH that hydrochloric acid is regulated fish meal and deionized water mixture; Add pepsin and trypsase then, pepsin and tryptic amount ratio are 10: 1, and pepsin and trypsase are 1:2500 with the mass ratio of fish meal and deionized water mixture; Then, under 37 ℃ condition, fish meal carried out 20 hours constant temperature hydrolysis; Collect hydrolyzate then through adsorbing with organic carrier after the centrifugal impurity elimination, namely make hydrolysis fish meal product after the drying; Wherein pepsic vigor is at least 4000IU/g, and tryptic vigor is at least 2500IU/mg;
C, soybean meal is being put into fermentation tank under 120 ℃ of conditions after 20 minutes high-temperature sterilizations are handled, inoculation is through bacillus and the Bacillus acidi lactici of activation culture in the fermentation tank then, and the mass ratio of soybean meal and bacillus and Bacillus acidi lactici is 100:1:2; Control fermentation initial temperature is 40 ℃, and material is 1: 1 with the volume ratio of water, and initial pH value is 7, obtains fermented product through 48 hours ferment at constant temperature, with fermented product after through 60 ℃ of constant temperature drying moisture fermented bean dregs; Wherein the concentration of bacillus is at least 1 * 10
9Individual/g, the concentration of Bacillus acidi lactici is at least 5 * 10
8Individual/g;
D, by kjeldahl determination and alkali nitrogen determination, to understand crude protein content and the free aminoacid content of said hydrolyzed fish meal and fermented bean dregs.
The said hydrolyzed fish meal is mixed the protein sources that forms feed with fermented bean dregs, the quality proportioning of hydrolysis fish meal and fermented bean dregs is 1:1.5, and protein sources and energy feed are uniformly mixed to form the stichopus japonicus feed, and the mass ratio of protein sources and energy feed is 1:3; Wherein energy feed is wheaten starch and seawood meal and ooze, and the quality proportioning of wheaten starch and seawood meal and ooze is 3: 3: 4.
Claims (2)
1. one kind is the method for major protein source preparation stichopus japonicus feed with hydrolysis fish meal and fermented bean dregs, and it is characterized in that: step is as follows:
A, be basic material and carry out pulverization process with fish meal and soybean meal, pulverize the back granularity and reach 200 orders;
B, fish meal is soaked with deionized water, the volume ratio of fish meal and deionized water is 1:2, soaking temperature is 25 ℃, and be 2.0 with the pH that hydrochloric acid is regulated fish meal and deionized water mixture, add pepsin and trypsase then, pepsin and tryptic amount ratio are 10: 1, pepsin and trypsase are 1: 2500 with the mass ratio of fish meal and deionized water mixture, then, under 37 ℃ condition, fish meal carried out 20 hours constant temperature hydrolysis, collect hydrolyzate through after the centrifugal impurity elimination with namely making hydrolysis fish meal product after organic carrier absorption, the drying; Described pepsic vigor is at least 4000IU/g, and tryptic vigor is at least 2500IU/mg;
C, soybean meal is carried out putting into fermentation tank after the sterilization treatment, in fermentation tank, inoculate bacillus and the Bacillus acidi lactici that passes through activation culture then, the mass ratio of soybean meal and bacillus and Bacillus acidi lactici is 100:1:2, control fermentation initial temperature is 40 ℃, material is 1: 1 with the volume ratio of water, initial pH value is 7, obtains fermented product through 48 hours ferment at constant temperature, with fermented product after through 60 ℃ of constant temperature drying moisture fermented bean dregs; The concentration of described bacillus is at least 1 * 10
9Individual/g, the concentration of Bacillus acidi lactici is at least 5 * 10
8Individual/g;
D, the hydrolysis fish meal is mixed the protein sources that forms feed with fermented bean dregs, the quality proportioning of hydrolysis fish meal and fermented bean dregs is 1:1.5, protein sources is mixed forming the stichopus japonicus feed with energy feed, and the mass ratio of protein sources and energy feed is 1:3.
2. according to claim 1 is the method for major protein source preparation stichopus japonicus feed with hydrolysis fish meal and fermented bean dregs, it is characterized in that: described energy feed is wheaten starch and seawood meal and ooze, and the quality proportioning of wheaten starch and seawood meal and ooze is 3: 3: 4.
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| CN2011100798643A CN102132789B (en) | 2011-03-31 | 2011-03-31 | Method for preparing stichopus japonicus feed by using hydrolyzed fish meal and fermented bean pulp as main protein source |
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| CN102132789B true CN102132789B (en) | 2013-08-21 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101449744A (en) * | 2007-11-30 | 2009-06-10 | 北京嘉博文生物科技有限公司 | Multifunctional biology aquatic feedstuff additive |
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| CN101449744A (en) * | 2007-11-30 | 2009-06-10 | 北京嘉博文生物科技有限公司 | Multifunctional biology aquatic feedstuff additive |
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