CN102960575A - Preparation method of oligopeptide bean pulp used for aquatic feed - Google Patents
Preparation method of oligopeptide bean pulp used for aquatic feed Download PDFInfo
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- CN102960575A CN102960575A CN2012105472461A CN201210547246A CN102960575A CN 102960575 A CN102960575 A CN 102960575A CN 2012105472461 A CN2012105472461 A CN 2012105472461A CN 201210547246 A CN201210547246 A CN 201210547246A CN 102960575 A CN102960575 A CN 102960575A
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Abstract
The invention discloses a preparation method of oligopeptide bean pulp used for an aquatic feed. The preparation method of the oligopeptide bean pulp used for the aquatic feed comprises the following steps of: smashing bean pulp; adding water, and homogenizing; carrying out heating pretreatment; regulating the temperate and pH value of a system; adding proteases for enzymolysis at constant temperature; controlling a hydrolyzing process; heating for enzyme deactivation; centrifugalizing, drying, and smashing so as to obtain the oligopeptide bean pulp used for the aquatic feed. The preparation method disclosed by the invention can effectively remove or passivate antinutritional factors contained in the oligopeptide bean pulp; the prepared oligopeptide bean pulp with the molecular weight of 180-2000 Da achieves oligopeptide content higher than 30%, has good viscoelasticity and can meet the requirement for processing of the aquatic feed; and the oligopeptide bean pulp produced by the preparation method disclosed by the invention is not only suitable for aquatic animal feeds, but also used for livestock and poultry feeds in large quantities, thereby reducing the production cost and enhancing the economic benefit.
Description
Technical field
The invention belongs to the feed preparation field, be specifically related to the preparation method that a kind of aquatic feeds is used the oligopeptides dregs of beans.
Background technology
Along with the fast development of aquaculture, the animal sources forage protein supply days such as fish meal are becoming tight, and developing new protein resource has become the significant problem that feed industry needs to be resolved hurrily.Utilize biocatalysis technology that dregs of beans albumen is changed into the focus that polypeptide, the contour value albumen of oligopeptides become current research.At present, existing a large amount of scholar's research improve the feeding quality of dregs of beans by the ANFs in the removal of the biochemical technologies such as fungi degradation, enzymolysis or the passivation dregs of beans both at home and abroad.But the product of its preparation is mainly paid close attention on the biological function of enzymolysis product, pursues high degree of hydrolysis, has ignored the processing characteristics of enzymolysis product.The special aquatic products feed not only requires high trophism, also need have the compatibility that good viscoelasticity is protein raw materials and a-starch; Excessively hydrolysis causes the part oligopeptides thoroughly to be hydrolyzed into free amino acid, affects the processing characteristics of hydrolysate; Hydrolysis is not enough, causes the digestibility of feed to reduce; Hydrolysis is not enough or the excessive needs that all can not satisfy the special aquatic products feed.
This method just is being based on this needs, screening by instrument protease and control index, the hydrolysis process of strict control dregs of beans, use two synchronous Hydrolyze method of enzyme defatted soybean meal is carried out enzymolysis, the oligopeptides dregs of beans of preparation not only has higher degree of hydrolysis and oligopeptides yield, and this product and a-starch has better compatibility, can satisfy the process requirements of special aquatic products feed.The oligopeptides dregs of beans of producing through this method is not only applicable to aquatic animal feed, also can use in a large number in animal and fowl fodder.The fish meal demand pressure will be greatly alleviated in the production of this product and application, promote the effective utilization of dregs of beans on the special aquaculture industry, to the aquaculture industry fast, stable, sustainable development is significant.
Summary of the invention
The object of the present invention is to provide a kind of aquatic feeds preparation method of oligopeptides dregs of beans, method of the present invention can effectively remove or the passivation dregs of beans in ANFs, the oligopeptides dregs of beans molecular weight of preparation is higher than 30% at the oligopeptides content of 180 ~ 2000Da, and have good viscoelasticity, can satisfy the process requirements of special aquatic products feed.The oligopeptides dregs of beans that the present invention produces is not only applicable to aquatic animal feed, also can use in a large number in animal and fowl fodder, thereby reduce production costs, and increases economic efficiency.
For achieving the above object, the present invention adopts following technical scheme:
A kind of aquatic feeds adds water homogenate, heating pretreatment with the preparation method of oligopeptides dregs of beans, dregs of beans through pulverizing, the temperature of regulation system and pH value add protease constant temperature enzymolysis, the controlled hydrolysis process, the heating enzyme that goes out, is pulverized at centrifugal, oven dry, namely makes aquatic feeds oligopeptides dregs of beans.Described preparation method may further comprise the steps:
(1) bean cake powder is broken to granularity and reaches 60～80 orders, adds water homogenate, and the mass concentration of substrate is 8～12%;
(2) the heating pretreatment temperature is 85 ~ 95 ℃, and be 15 ~ 30 min heat time heating time;
(3) temperature to 50 of regulation system ~ 60 ℃, pH value to 6.0 ~ 8.0;
(4) add protease constant temperature enzymolysis, described protease is the composition of a kind of and flavor protease in alkali protease, the papain, and enzyme is lived than being 1:1～2:1; Enzyme concentration is 3000 ~ 4000 U/g; Enzymolysis time is 7.5 ~ 9 h.
Alkali protease and papain belong to endo protease, and action site is extensive, have guaranteed to a certain extent the abundant hydrolysis of dregs of beans albumen; Flavor protease has the function of restriction endonuclease and excision enzyme simultaneously concurrently, and its enzymolysis product has lower bitterness value and higher local flavor; This combination is carried out synchronous enzymolysis to dregs of beans, not only can the Effective Raise hydrolysis efficiency, improve the palatability of feed, and because catalytic site is different, the nutritive peculiarity of enzymolysis product and physiological function be horn of plenty more.
Described controlled hydrolysis process, foundation is controlled the hydrolysis process of the standby oligopeptides dregs of beans of the synchronous legal system of two enzymes take the oligopeptides yield as the control parameter, determine enzymolysis time with oligopeptides or oligopeptides yield optimal result, solved preferably the not enough or excessive problem of hydrolysis in the proteolysis process.Oligopeptides bean cake powder and the a-starch of preparation have preferably compatibility, and scatter ratio, scatter and disappear ratio is less than 5%, and process quality index can reach the aquaculture feed processing request.
Because flavor protease includes the multiple protein enzymes such as endo protease and exoproteinase, not only act on the peptide bond of peptide chain inside, and act on the peptide bond of peptide chain outside, long enzymolysis time easily causes the part oligopeptides thoroughly to be hydrolyzed into free amino acid.
The product that the present invention obtains is specially adapted to special aquatic products forage protein source, can also be used as the feed of cultivated animals simultaneously, such as poultry, domestic animal etc.
Beneficial effect of the present invention is: utilize the nutritive peculiarity of peptide albumen and mode and the hydrolysis degree of processing characteristics and its hydrolysis that close correlation is arranged, hydrolytic process is strictly controlled.With degree of hydrolysis as control index, although in the apparent control that has realized proteolysis, but ignored the selective of difference that protein forms, protease, cause easily excessive hydrolysis, may cause the part oligopeptides thoroughly to be hydrolyzed into free amino acid, not only reduce nutritive value, also destroyed the processing characteristics of product simultaneously; Take the molten albumen of acid (or nitrogen solubility index) as index, consequently reflect molecular weight less than the soluble peptide of 10 kDa, easily cause hydrolysis not enough, cause the digestibility of feed to reduce; Hydrolysis is not enough or excessively all can not reach ideal effect.For these reasons, the present invention's proposition as the control index, is carried out appropriate enzymolysis to defatted soybean meal with oligopeptides content.Utilize the oligopeptides dregs of beans of this method preparation not only to have higher degree of hydrolysis and oligopeptides yield, and this product and a-starch has better compatibility, can satisfy the process requirements of special aquatic products feed.
The specific embodiment
Basic ideas of the present invention are to utilize the nutritive peculiarity of peptide albumen and mode and the hydrolysis degree of processing characteristics and its hydrolysis that close correlation is arranged, compare by selecting suitable instrument proteinase combination and enzyme to live, take the oligopeptides yield as the control parameter, hydrolytic process is strictly controlled.Basic process: dregs of beans adds water homogenate by a certain percentage through pulverizing, and heating pretreatment transfers to suitable temperature, pH value, adds protease constant temperature enzymolysis, heats the enzyme that goes out, oven dry, pulverizing.
Embodiment 1:(alkali protease and flavor protease)
(1) gets 1 kg defatted soybean meal, add 10 L water homogenate, 90 ℃ of lixiviate 30 min.
(2) with the homogenate pH to 8.0 in the 1 mol/L NaOH regulating step 1, transferring system temperature is 60 ℃.
(3) press enzyme work than (alkali protease: flavor protease) 2:1 than 3000 U/g, adds alkali protease and flavor protease mixed liquor at the bottom of the enzyme.
(4) 60 ℃ of insulation hydrolysis 7.5 h.
(5) 90 ℃ of enzyme 10 min that go out, the solution oven dry, bake out temperature is 70 ℃.
(6) with the sample of oven dry, utilize the Cyclone mill grinds, namely make finished product.
Embodiment 2:(papain and flavor protease)
(1) gets 1 kg defatted soybean meal, add 10 L water homogenate, 90 ℃ of lixiviate 30 min.
(2) with the homogenate pH to 7.0 in the 1 mol/L NaOH regulating step 1, transferring system temperature is 45 ℃.
(3) press enzyme work than (papain: flavor protease) 1:1 than 4000 U/g, adds papain and flavor protease mixed liquor at the bottom of the enzyme.
(4) 45 ℃ of insulation hydrolysis 9 h.
(5) 90 ℃ of enzyme 10 min that go out, the solution oven dry, bake out temperature is 70 ℃.
(6) with the sample of oven dry, utilize the Cyclone mill grinds, namely make finished product.
The product processing quality check
Make two kinds of oligopeptides soybean products, dissolve-loss ratio is estimated and measured to its viscoelasticity.
Embodiment 1: product have good viscoelasticity and extensibility, volume obviously expands behind the drawn, can bear pressure, it is uniform sequential to be launched into thin shape, by very fast bounce-back recovery and tack-free after recessed, dissolve-loss ratio is 2.5%, shows that this product and a-starch have better compatibility, can satisfy the requirement of special aquatic products feed process quality index.
Embodiment 2: product have good viscoelasticity and extensibility, dissolve-loss ratio is 3.8%, reaches fishing with the requirement of mixed feed powder dissolve-loss ratio≤5%.
The above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. an aquatic feeds is with the preparation method of oligopeptides dregs of beans, it is characterized in that: dregs of beans is through pulverizing, add water homogenate, heating pretreatment, the temperature of regulation system and pH value add protease constant temperature enzymolysis, the controlled hydrolysis process, the heating enzyme that goes out, is pulverized at centrifugal, oven dry, namely makes aquatic feeds oligopeptides dregs of beans.
2. aquatic feeds according to claim 1 is with the preparation method of oligopeptides dregs of beans, and it is characterized in that: described preparation method may further comprise the steps:
(1) bean cake powder is broken to granularity and reaches 60～80 orders, adds water homogenate, and the mass concentration of substrate is 8～12%;
(2) the heating pretreatment temperature is 85 ~ 95 ℃, and be 15 ~ 30 min heat time heating time;
(3) temperature to 50 of regulation system ~ 60 ℃, pH value to 6.0 ~ 8.0;
(4) add protease constant temperature enzymolysis, described protease is the composition of a kind of and flavor protease in alkali protease, the papain, and enzyme is lived than being 1:1～2:1; Enzyme concentration is 3000 ~ 4000 U/g; Enzymolysis time is 7.5 ~ 9 h.
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Cited By (6)
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| CN103349175A (en) * | 2013-06-22 | 2013-10-16 | 福建省农业科学院中心实验室 | Powdery formulated feed for anguilla marmorata overwintering |
| CN104187174A (en) * | 2014-09-15 | 2014-12-10 | 中国科学院青岛生物能源与过程研究所 | Process for producing livestock feed additive from reside and dreg materials |
| CN104543568A (en) * | 2015-01-12 | 2015-04-29 | 福建省农业科学院中心实验室 | Pelteobagrus fulvidraco feed rich in small peptide |
| CN107484921A (en) * | 2017-08-24 | 2017-12-19 | 浦江县协盈动物饲料技术开发有限公司 | The preparation method of shallow sea fish fry phase mixed feed |
| CN108056219A (en) * | 2017-12-08 | 2018-05-22 | 武汉新华扬生物股份有限公司 | Remove bitter taste fermented bean dregs and preparation method thereof |
| CN112956645A (en) * | 2021-03-05 | 2021-06-15 | 克东禹王大豆蛋白食品有限公司 | Production device of low-temperature defatted soybean meal with high whiteness and low beany flavor and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103349175A (en) * | 2013-06-22 | 2013-10-16 | 福建省农业科学院中心实验室 | Powdery formulated feed for anguilla marmorata overwintering |
| CN103349175B (en) * | 2013-06-22 | 2014-12-10 | 福建省农业科学院中心实验室 | Powdery formulated feed for anguilla marmorata overwintering |
| CN104187174A (en) * | 2014-09-15 | 2014-12-10 | 中国科学院青岛生物能源与过程研究所 | Process for producing livestock feed additive from reside and dreg materials |
| CN104543568A (en) * | 2015-01-12 | 2015-04-29 | 福建省农业科学院中心实验室 | Pelteobagrus fulvidraco feed rich in small peptide |
| CN107484921A (en) * | 2017-08-24 | 2017-12-19 | 浦江县协盈动物饲料技术开发有限公司 | The preparation method of shallow sea fish fry phase mixed feed |
| CN108056219A (en) * | 2017-12-08 | 2018-05-22 | 武汉新华扬生物股份有限公司 | Remove bitter taste fermented bean dregs and preparation method thereof |
| CN112956645A (en) * | 2021-03-05 | 2021-06-15 | 克东禹王大豆蛋白食品有限公司 | Production device of low-temperature defatted soybean meal with high whiteness and low beany flavor and preparation method thereof |
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Application publication date: 20130313 |