CN102057881B - Method for verifying quality of young hippocampus quickly - Google Patents
Method for verifying quality of young hippocampus quickly Download PDFInfo
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- CN102057881B CN102057881B CN2009101988262A CN200910198826A CN102057881B CN 102057881 B CN102057881 B CN 102057881B CN 2009101988262 A CN2009101988262 A CN 2009101988262A CN 200910198826 A CN200910198826 A CN 200910198826A CN 102057881 B CN102057881 B CN 102057881B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for verifying the quality of young hippocampus quickly, relates to a quality verification technique of young hippocampus, and an index and quick verification method for verifying the quality of the young hippocampus are required to be provided. The method for verifying the quality of the young hippocampus quickly is characterized by comprising: taking new hatched young hippocampus randomly and putting in three 25-40 litre aquriums, wherein the density of young hippocampus arrangement is 2 tails per litre; feeding new hatched artemia nauplius twice every day, and feeding for 21 days; taking 5 tails every 3 days randomly before and after young hippocampus arrangement so as to measure the body height, the trunk height, and the width at 1/2 of the trunk height; observing every day, removing promptly if new hatched young hippocampus is died, and measuring the body height, the trunk height, and the width at 1/2 of the trunk height; calculating the ratio W/H of the width at 1/2 of the truck height to the trunk height, and comparing the differences of two indexes between living individuals and died individuals, thus determining the ratio of the width at 1/2 of the trunk height of the body index to the trunk height, wherein the ratio represents the index of the quality of the young hippocampus; and measuring the W/H data so as to verify the quality of the young hippocampus. The method for verifying the quality of the young hippocampus quickly is used for verifying the quality of the young hippocampus.
Description
Technical field
The present invention relates to hippocampus seedling Quality Identification technology.
Background technology
Hippocampus is traditional rare Chinese medicine.According to statistics, dry product is used as medicine every year over 150 tons in China.Because Natural Resources in China is closely exhausted, the required hippocampus import most of existing market, price raises year by year, and average price surpasses 10,000 yuan per jin, according to 150 tons of calculating of annual import, is worth 3,000,000,000 yuan.Because the hippocampus natural resources is seriously damaged, be to recover resource, many countries have carried out and have propagated research artificially in the world.China namely carries out the hippocampus culture research as far back as nineteen fifty-seven, past 50 years, though seedling growing process has development, mass production techniques does not obtain breakthrough yet.At present, hippocampus to propagate the greatest problem that runs into artificially be that survival rate of seedling is low.Hippocampus seedling quality is to determine to grow seedlings the key of success or not.If can know in advance the quality of seedling, just can determine seedling values of whether withing a hook at the end according to the quality of seedling before growing seedlings, or determine to take which kind of measure raising survival rate of seedling in seedling raising process.
In fish hatching, generally detect the quality of ovum, as Development of egg, incubation rate etc.If abnormal rate is too high and incubation rate is too low, the seedling of this ovum or new hatching will be given it up, thus of low quality just the stopping of just finding seedling after a period of time of needn't by the time growing seedlings grow seedlings, but cost saving.But line hippocampus propagating system has its particularity, and male hippocampus is undertaken the child-bearing task.Male and female hippocampus post-coitum, female hippocampus produce ovum fertilization in the child-bearing capsule of male hippocampus, grow until hatching is emerged.The quality of ovum can't be judged before hatching.Therefore, the quality of Rapid identification line hippocampus seedling is significant to raising survival rate of seedling, reduction seedling cost.
Summary of the invention
The technical issues that need to address of the present invention are to provide index and the rapid identification method that can measure line hippocampus seedling quality.
Technical scheme of the present invention: get at random that 10 tails measure that heights, trunk are high, the width at trunk high 1/2 place; The line hippocampus seedling of getting at random new hatching is put into 3 25-40 and is risen in aquarium, puts every liter of 2 tails of seedling density; The artemia nauplii of throwing something and feeding every day and newly hatching for 2 times was fed 21 days; Put after seedling got at random that 5 tails measure that heights, trunk are high in every 3 days, the width at trunk high 1/2 place; Whether have dead individual, if any death, take out immediately and measure the width (W) at high 1/2 place of height, trunk high (H), trunk if observing every day; Calculate the width at trunk high 1/2 place and the ratio (W/H) of trunk height, relatively survival individual with dead individuality between the difference of this index, thereby the width at definite build index trunk high 1/2 place and the ratio of trunk height represent the index of line hippocampus seedling quality; And measure the W/H data and identify line hippocampus seedling quality.
The outstanding feature of this method is to identify the quality of line hippocampus seedling according to the width at build index trunk high 1/2 place and the ratio (W/H) of trunk height.Although IC also can illustrate the quality of seedling, need valuable precision balance to weigh.This type of balance is enervated, maintains bad error large, and general plant does not satisfy the requirements.And weight in wet base test macro error is large, can affect the confidence level of IC.Method of the present invention is easy, as long as once dealing with problems chi, systematic error is little, and calculates simple.In addition, the time is short, only needs can to determine in 3 days the difference of width with the ratio of trunk height at individual trunk high 1/2 place, thereby can determine the quality of seedling.
Description of drawings
Accompanying drawing represents be line hippocampus (Hippocampus erectus) dead with the width at survival young trunk high 1/2 place and the relation of trunk high (W/H) and height.
Embodiment
The below illustrates the overall process of implementing this method as an example of line hippocampus (Hippocampus erectus) example.
After line hippocampus hatching, get at random that 10 tails measure that heights, trunk are high, width, the weight in wet base at trunk high 1/2 place.The line hippocampus seedling of getting at random new hatching is put into 3 25-40 and is risen in aquarium, puts every liter of 2 tails of seedling density.The artemia nauplii of throwing something and feeding every day and newly hatching for 2 times was fed 20 days.Put after seedling 3 days, 7 days, 14 days and 20 days each get at random that 10 tails measure that heights, trunk are high, the width at trunk high 1/2 place.Observe every day whether dead individuality is arranged.If any death, take out immediately and measure that height, trunk are high, the width at trunk high 1/2 place.The width at high 1/2 place of calculating trunk and the ratio (W/H) of trunk height, the relatively difference of this index between survival individuality and dead individuality.
Can find from Fig. 1 test result, dead individual W/H significantly (P＜0.05) lower than normal individual, illustrates that W/H also can be used as the quality index of line hippocampus seedling.
In order to confirm the validity of this index, another line hippocampus seedling with newly hatching is put every liter of 2 tails of seedling density in the cylinder of 3 30 liters, the artemia nauplii of 2 the new hatchings of throwing something and feeding every day.Cultivate after 3-4 days, the obvious thin seedling (being the little seedling of W/H) of body in 3 cylinders is moved on in another cylinder, make the density of 4 cylinders close.Select at random 10 tails to measure the width at high 1/2 place of the high and trunk of trunks, continue cultivation 14 days, compare the survival rate of field run plant and the thin seedling of body.The result demonstration, the survival rate of the seedling that W/H is little (the thin seedling of body) is significantly lower than the high seedling of W/H.Result confirms that further W/H can be used as line hippocampus seedling quality Rapid identification index, and cultivates about 3 days and can tell the low quality seedling.
Claims (1)
1. line hippocampus seedling quality rapid identification method, the line hippocampus seedling that it is characterized in that getting at random new hatching is put into 3 25-40 and is risen in aquarium, puts every liter of 2 tails of seedling density; The artemia nauplii of throwing something and feeding every day and newly hatching for 2 times was fed 21 days; Before putting seedling, put after seedling got at random that 5 tails measure that heights, trunk are high in every 3 days, the width at trunk high 1/2 place; Whether have dead individual, if any death, take out immediately and measure that height, trunk are high, the width at trunk high 1/2 place if observing every day; Calculate the width at trunk high 1/2 place and the ratio W/H of trunk height, relatively survival individual with dead individuality between the difference of this index, thereby the width at definite build index trunk high 1/2 place and the ratio of trunk height represent the index of line hippocampus seedling quality; And measure the W/H data and identify line hippocampus seedling quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101988262A CN102057881B (en) | 2009-11-16 | 2009-11-16 | Method for verifying quality of young hippocampus quickly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101988262A CN102057881B (en) | 2009-11-16 | 2009-11-16 | Method for verifying quality of young hippocampus quickly |
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| Publication Number | Publication Date |
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| CN102057881A CN102057881A (en) | 2011-05-18 |
| CN102057881B true CN102057881B (en) | 2013-05-08 |
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| CN2009101988262A Expired - Fee Related CN102057881B (en) | 2009-11-16 | 2009-11-16 | Method for verifying quality of young hippocampus quickly |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1219430C (en) * | 2001-04-09 | 2005-09-21 | 中山大学 | Youngling of large sea horse and artificial cultivating method of commodity sea horse and its equipment |
| CN101401554A (en) * | 2007-10-07 | 2009-04-08 | 常庆发 | Artificial cultivation method for sea horse fry |
| CN101248769A (en) * | 2008-03-31 | 2008-08-27 | 李秉记 | Feeding method of bait for breeding Hippocampus trimaculatus Leach |
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Granted publication date: 20130508 Termination date: 20201116 |