CN105123621A - Establishment method of goat resource population - Google Patents
Establishment method of goat resource population Download PDFInfo
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- CN105123621A CN105123621A CN201510684788.7A CN201510684788A CN105123621A CN 105123621 A CN105123621 A CN 105123621A CN 201510684788 A CN201510684788 A CN 201510684788A CN 105123621 A CN105123621 A CN 105123621A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention belongs to the field of establishment of a population genetic resource bank, and provides a scientific establishment method of a goat resource population bank, and provides a goat reference family system establishment case, and relates to two national-level genetic resource varieties, namely, a Dazu black goat and an Inner Mongolia Cashmere goat (Alxa type). According to the resource population establishment method, forward cross and reciprocal cross of Dazu black goat and Inner Mongolia Cashmere goat (Alxa type) are utilized, random mating of genetic relationship is avoided for F1, so that Mendel genetic characters are separated, a goat economic character QTL or a functional gene is located by virtue of a genome means, and a relatively-precise genetic mark is provided for the goat breeding. The genetic resource bank comprises goat population, a genealogy inforamtion database, a phenotypic character database, a DNA sample library, an RNA sample library and a genetic variation information database.
Description
Technical field
The present invention relates to a kind of goat sources group construction method and genes of individuals group variation information database structure thereof.
Background technology
Goat (
caprahircusone of) domestic animal raised and train the earliest as the mankind, extensively raised in worldwide, especially in developing countries such as China, India, within 2013, only whole world goat cultivation quantity is more than 1,000,000,000 according to estimates.Goat can provide the products such as meat, hair (suede), skin, milk, casing for the mankind, and is one of food of the Moslem people, so the sound development of sheep husbandry is significant with promotion national unity to raising people level.Goats Breeds is the backbone ensureing that sheep husbandry develops in a healthy way, but in world wide, goat genetic improvement is made slow progress.Along with completing of the Human Genome Project, Animal genome plan also develops into a critical period, and the technology of the Human Genome Project, method and thinking are applied to Animal genome field by increasing research.Differently with the Human Genome Project be, Animal genome focuses on genetic map and the physical map of drawing out control livestock and poultry proterties, thus the quantitative trait locus (Quantitativetraitloci of accurate positioning effects livestock and poultry proterties, QTL) and excavate advantage proterties functional gene, then in conjunction with DNA recombinant technique, these information are used for improveing livestock and poultry species.
China is goat cultivation big country, and its cultivation is with a long history, kind (or genetic resources) aboundresources, according to statistics the existing Goats Breeds of China 70 (Zhao Youzhang, Chinese sheep husbandry, 2013).China goat raising is in recent years raised scattered by individuality and is made the transition to large-scale breeding; along with goat cultivation scale, intensively to improve constantly; kind is single, integral production level is difficult to the industry development predicaments such as raising and highlights; seriously hinder the sustainable health development of China's goat industry; be badly in need of efficiently breeding etc. around the rational exploitation and utilization of goat genetic resources, breeding carrying out cutting edge technology research, deeply understand molecular mechanism and the qualification target gene of complex character.
Black goat is gained the name because originating in great Zu district of Chongqing City, belongs to meat and fur Local Excellent Goats Breeds.Male and female sheep birth weight is respectively 2.2kg and 2.1kg, and 6 monthly ages were heavily respectively 22.3kg and 19.0kg, one full year of life male and female sheep be respectively 37.6kg and 27.7kg, grow up male and female sheep body weight be respectively 59.6kg and 40.2kg.12 the monthly age Slaughter weight male and female sheep be respectively 35.10kg and 24.04kg, male and female sheep dressing percentage is respectively 44.93% and 44.72%, and pure meat percentage is respectively 34.24% and 33.18%.This population has early sexual maturity, fundamental characteristics that fertility is high.Namely ram shows sexual behavior about 2 ~ 3 monthly ages, and generally just joining the age is 7 ~ August; There are just feelings at 3 monthly ages in ewe, 6 monthly ages started breeding.The average lambing percentage of primiparity ewe is 218.0%, 2 ~ 6 tire multiparity ewe lambing percentages is 272.2%.
Cashmere Goat mainly originates in China and west Mongolia.Its producing region, center is temperate zone extreme continental climate, and winter is very long and cold, and summer is warm and very brief, drought, and wind great Sha is many, and day and night temperature is large.Cashmere Goat grows up that the average height of ram, body are long, chest measurement and body weight are respectively 65.4cm, 70.8cm, 85.1cm, 47.8kg, and adult ewe is respectively 56.4cm, 59.1cm, 70.7cm, 27.4kg, dressing percentage 40% ~ 50%.Cashmere Goat clip ram average out to 570g, ewe average out to 257g; Grab suede amount to grow up ram average out to 385g, grow up ewe average out to 305g.Villus length ram average out to 7.6cm, ewe average out to 6.6cm; Fine hair fineness ram average out to 14.6 μm, ewe average out to 15.6 μm; Coarse wool length ram average out to 17.6cm, ewe average out to 13.5cm.This variety breeding rate is low, voluminous single lamb, and lamb development is fast, and survival rate is high.Lambing percentage 100% ~ 105%.
Black goat and Cashmere Goat are produce meat and produce suede for main kind respectively, are the important genetic resourcess of China.The present invention is to set up Black goat and Cashmere Goat (Alxa type) reference family for case, create a kind of goat sources group construction method, SNPs, SV and CNVs is excavated, research complex character Genetic Mechanisms in conjunction with genome research technology, method and thinking; Conbined usage DNA(SNPs, SV and CNVs etc.), RNA(express spectra, microRNA), the information of proteomics, deeply understand molecular mechanism and the qualification target gene of complex character; Utilize existing breeding system, cultivate the new varieties with advantage performance in conjunction with genome selection, fast breeding technique.Thus provide strong science and technology support for the development of China Modern Animal Husbandry, realize healthy aquaculture quantity, quality and benefits lays equal stress on.
Summary of the invention
The object of the invention is to: one scientifically goat sources group construction method is provided, the constructing plan of case Black goat and Cashmere Goat (Alxa type) reference family is provided.
The present invention has built national genetic resources kind---and Black goat and Cashmere Goat (Alxa type) reference family, wherein information comprises: pedigree information database, phenotype trait data storehouse, tissue samples storehouse, DNA sample storehouse, RNA Sample Storehouse and genetic variation information database.Reference family case provided by the invention has gathered the basic sign information of 50 goats (wherein ewe 40, ram 10) and blood sample 50 parts at present, and completes typing and the sample gene group DNA extraction of sign information database and preserve.
This goat sources group has feature: 1, Research Significance is obvious, and Black goat and Cashmere Goat (Alxa type) all belong to national genetic resources, and breed improvement contributes to improving national economy level and international competition strength; 2, composition of sample contrast obviously, and Black goat and Cashmere Goat (Alxa type) are obvious by many-sided differences such as hair color, production mode reform, reproductive performances, and positive and negative friendship can impel Gene Isolation, has to utilize and find objective trait QTL or functional gene; 3, all information, preservation complete, can for continuing research, the correlated traits records such as the sources group pedigree information that the present invention relates to, sign information, cashmere production, meat performance, reproductive performance and skin performance are perfect, tissue samples, RNA and DNA sample are included complete, single nucleotide polymorphism (Singlenucleotidepolymorphism, SNP), structure variation (Structuralvariation, and the copy number variation Database structural integrity such as (Copynumbervariation, CNV) SV); 4, build opportunity appropriate, in conjunction with nearly 2 years fast-developing omics technologies (especially DNA sequencing technology), goat with reference to genome and goat superchip, goat economic characters functional gene can be excavated fast.
Technical scheme of the present invention: a kind of construction method of goat sources group storehouse.The concrete steps building goat genetic resource are as follows:
One, sources group constructing plan and data logger is drafted, comprise specific embodiments, introduce a fine variety scheme (comprise and introduce a fine variety points for attention and breed standard), Goats Breeds resource investigation individual registration form, sample collection consumptive material, habitus index survey tool, staff divides into groups.
Two, buy consanguinity-less relation Black goat and Cashmere Goat, carry out sources group structure according to the constructing plan of Fig. 1.
Three, build group researcher carries out overbit establishment to the individuality involved by sources group, and the collection of basic sign information record.
Four, suede grinds group researcher and treats that the down producing goat lint in sources group carries out grabbing suede season, and records related data record.
Five, breeding group researcher carries out mensuration and the record of reproductive trait respectively to the male and female sheep in sources group, and gather placenta, Cord blood sample is some, be stored in be equipped with RNA protect liquid cryopreservation tube in.
Six, meat ground group researcher carry out meat performance information when goat is butchered, and it is some to gather the position samples such as the heart, liver, spleen, lung, kidney, muscle, skin, brain, cerebellum and hypophysis, data record.
Seven, skin grind group researcher the collection of skin proterties is carried out and record to the goat individuality in sources group.
Eight, blood sampling group researcher carries out jugular vein blood collection (multi collect) to all individualities of sources group when 6 monthly age, adopts liquaemin anti-freezing, ACD anti-freezing and EDTA-Na respectively
2anti-freezing, is numbered blood sample and registers related content.
Nine, experiment processed group I is responsible for extracting meat and is ground the tissue samples RNA of group collection and the blood sample DNA of blood sampling group collection.RNA is placed in and the cryopreservation tube that RNA protects liquid is housed, and pipe shaft indicates No. ID, sample and extracts the date, and fills in RNA sample information table; DNA sample adopts TEBuffer dissolve and be stored in 1.5ml cryopreservation tube, and pipe shaft marks No. ID, sample respectively and extracts the date, and fills in DNA sample information table.
Ten, test processed group II to be responsible for arranging gene order-checking and chip gene somatotype, excavate the genetic variation information such as SNP, SV and CNV.
11, sources group pedigree information database, phenotype trait data storehouse, tissue samples storehouse, RNA Sample Storehouse, DNA sample storehouse and genetic variation information database is set up: seal up for safekeeping after the bookbinding of genetic resources all original written information numberings, information material is stored in computer, the full and accurate information database of system is set up with Access software, to imprint CDs preservation simultaneously, genetic variation variation database is positioned on the ftp server oneself built, and backs up and preserve in hard disk.All databases are in charge of the management by full-time researcher.
12, the goat sources group information data library directory that collects also is worked out all kinds of sample and is received rule.
In step 3, the basic sign information of build group collection should comprise following information: by hair color, the colour of skin, body body, angle-style, wattle, wrinkle, beard, nose, shank, ear type, hoof matter, style of the shoe, four limbs, udder shape, whether nipple is even, with or without additional teat, angular length (cm), ear length (cm), ear wide (cm), height (cm), body weight (kg), chest measurement (cm), circumference of cannon bone (cm), hip width (cm), testis encloses (cm), long (cm), the head breadth (cm), body long (cm), chest breadth (cm), chest depth (cm), hip cross high (cm), see Fig. 2.
Step 4 medium staple grinds group collection proterties and should comprise: hair color, suede look, fine hair ratio, cashmere length (μm), cashmere diameter (μm), cashmere density (root/cm
2), pile layer height (cm), cashmere intensity (CN), clean suede rate, is shown in Fig. 3.
The reproductive performance information of step 5 breeding group collection should comprise: lambing type, lamb sex, parity, birth weight (g), cotyledon < 1cm, cotyledon >=2-3cm, cotyledon >=1-2cm, cotyledon >=4-5cm, cotyledon >=3-4cm, cotyledon >=5cm, Placental Weight (g), the wide footpath of cotyledon (cm), testis encloses (cm), sperm concentration, ejaculatory amount (μ L), sperm viability, rate of teratosperm, acrosomal integrity, sperm viability, sperm survival index, seminal fluid pH value, seminal fluid osmotic pressure, GOT(glutamic-oxalacetic transaminease) burst size, conception rate, see Fig. 4.
In step 6, meat grinds group acquisition index and should comprise: birth weight (g), fatten starting weight (kg), weaning weight (kg), fatten whole weight (kg), 6 monthly ages heavy (kg), Slaughter weight (kg), age one full year of life heavy (kg), carcass weight (kg), 18 monthly ages heavy (kg), dressing percentage, grow up heavy (24 monthly age) (kg), trunk pure meat percentage, carcass quality, the thickness of backfat (cm), yellowish pink (5 points of systems), eye muscle area (cm
2), marbling (12 points of systems), plate thick (GR value), fat color (7 points of systems), meat bone ratio, percentage of water loss, pH value, storage loss rate, tenderness (shearing force), lean meat percentage, meat bone ratio, see Fig. 5.
In step 7, skin grinds group and should gather following information: wadding of wool proterties, silk, wadding of wool size, staple length mensuration, flower case area, flower case definition, Wool density, hide thickness, identity area, plate matter, hair color, decorative pattern width, bending, pattern, gloss, the hair color uniformity, hide area, tare weight etc., sees Fig. 6.
In step 8, blood sampling group gathers blood and should be 5ml at every turn, and each blood sample gathered should extract genomic DNA in time, if short-term preservation can be placed in-20 DEG C of refrigerators, preserves will be placed in-80 DEG C of refrigerators if long-term.
The genome DNA extracting method that step 9 relates to is phenol-chlorine extraction process, and DNA sample information record sheet should comprise following content: overbit, Guan Hao, kind, tissue, anticoagulant, extracting method, lysate, OD260/280, OD260/230, concentration C (ng/ μ l), information such as extraction date, DNA volume etc.; RNA extracts to grind after group has adopted sample at step 6 meat and extracts immediately; extract and adopt sky root RNA to extract kit; the RNA added protects liquid to be TRIzol, and RNA sample information record sheet should comprise following content overbit, Guan Hao, kind, tissue, extracting method, lysate, OD260/280, OD260/230, concentration C (ng/ μ l), extract the information such as date, volume.
The genetic variation data that step 10 relates to deposit in ftp server according to dissimilar type.
All information equal typing Access database involved by step 3 to step 10, and according to different classes of preservation of imprinting CDs.
Accompanying drawing explanation
Fig. 1 is sources group constructing plan design drawing;
Fig. 2 is the basic sign information record sheet of sources group;
Fig. 3 is that sources group produces suede characteristics record table;
Fig. 4 is sources group reproductive performance record sheet;
Fig. 5 is sources group meat-producing traits record sheet;
Fig. 6 is sources group skin characteristics record table;
Fig. 7 is DNA sample information record sheet;
Fig. 8 is RNA sample information record sheet;
Fig. 9 is phenotype trait data storehouse sectional drawing.
Embodiment
Build below in conjunction with Black goat of the present invention and Cashmere Goat (Alxa type) reference family that the invention will be further described, but not as limitation of the present invention.
Goat sources group builds case: Black goat and Cashmere Goat (Alxa type) reference family (DIReferenceFamily, DIRF) build, and carry out according to the following steps:
One, inspection information, draft goat reference family constructing plan, formulate breeding record sheet, basic sign information record sheet, cashmere production record sheet, meat performance record sheet, reproductive performance record sheet and skin performance inventory table, prepare reagent consumptive material needed for sample collection, and organize relevant staff.
Two, buy consanguinity-less relation, meet the Black goat ram 5 of breed standard, ewe 20, Cashmere Goat (Alxa type) ram 5, ewe 20, using these 50 goats as parental generation (P).
Three, adopt synchronization of Estrus, be divided into 3 groups, use Black goat ram (D) to carry out orthogonal with Cashmere Goat (I) ewe respectively by male and female sheep 1:4, large black goat ewe (D) and Cashmere Goat ram (I) carry out reciprocal cross and obtain F1 generation.
Four, positive and negative friendship is that offspring's male and female sheep avoids affiliation to carry out random mating obtaining F2 generation, and constructing plan is shown in Fig. 1.
Five, prepare all kinds of performance and gather consumptive material, mainly comprise: breeding record sheet, basic sign information record sheet, cashmere production record sheet, meat performance record sheet, reproductive performance record sheet, skin performance gloves, mouth mask, record sheet, pen, tape measure, ruler, biltmore stick, pelvis device, template, PH test paper, weighing balance, syringe, liquaemin anticoagulant tube, EDTA-Na
2anticoagulant tube and ACD anticoagulant tube etc.
Six, performance collector is grouped into: build group, suede grind group, meat grinds group, breeding group, skin grind group, blood sampling group, experiment processed group I and II.
Seven, build group membership when goat is born, 2 monthly ages, 6 monthly ages, 12 monthly ages and 24 monthly ages carry out basic sign generaI investigation and record to all individualities of sources group respectively.
Eight, suede grinds group and carries out grabbing suede season in goat lint every year, and all characteristic indexs measured involved by cashmere production placing on record.
Nine, meat grinds group and is responsible for collecting reference family individual growth proterties, measures and records the index that all meat performance record sheets relate to, and be responsible for each tissue sample of collection when sources group goat is butchered.
Ten, breeding group is responsible for performing the sympathy relating to reference family and is oestrused all reproductive trait parameters of technology, record reproductive performance record sheet.
11, skin grinds the responsible index gathered involved by all reference families individual skin performance inventory table of group.
12, blood sampling group gathers jugular vein blood (can multi collect) at 6 monthly ages, adopts liquaemin anti-freezing, ACD anti-freezing and EDTA-Na respectively
2anti-freezing, is numbered blood sample and registers related content.
13, test processed group I to be responsible for grinding to blood sampling group and meat the sample that group gathers and to carry out blood sample genomic DNA and organize sample RNA to extract, No. ID, check sample to preserve sample for subsequent use.
14, test processed group II to be responsible for arranging all genes of individuals group order-checkings of reference family and chip gene somatotype, excavate the variation information such as SNP, SV and CNV.
15, set up pedigree information database: the breeding record sheet of compilation reference family, arrange result establishment pedigree information data.
16, set up tissue samples storehouse: meat is ground the tissue samples that group gathers and load in aseptic cryopreservation tube, pipe shaft indicate No. ID, organization name, collection date etc., all tissue samples are placed in Liquid nitrogen storage according to a definite sequence, in order to the use of DNA or RNA extraction.
17, reference family DNA sample storehouse is set up: be stored in respectively after being checked digit by the sample genomic dna that experiment processed group is extracted (each individuality preserves 2 parts of genomic DNA samples) in-80 DEG C of ultra low temperature freezers.
18, reference family RNA Sample Storehouse is set up: be stored in liquid nitrogen for subsequent use after being checked digit by the RNA sample that experiment processed group is extracted.
19, reference family phenotype trait data storehouse is set up: comprise papery version database and electronics profile database.Papery version database is sealed up for safekeeping according to different classes of unification and is deposited; Electronics profile database, based on papery version record, has been formulated by Access software, and preservation of imprinting CDs.And the record management work of database is responsible for by full-time researcher.
20, set up reference family genetic variation information database: carry out gene order-checking or chip scanning with reference to all individualities of family, excavate the variation such as SNP, SV and CNV, by these variation data according to variation type with individual ID is sub-category deposits, for future use.
21, Black goat and Cashmere Goat (Alxa type) reference family total data storehouse (referred to as DIRF database) is set up: total data storehouse comprises pedigree information database, tissue sample storehouse, phenotype trait data storehouse, DNA sample storehouse, RNA Sample Storehouse and genetic variation information database, compilation total data library directory, formulation total data storehouse is checked and is received rule and management method.
Step 9 meat grinds group collection tissue and comprises: the heart, liver, spleen, lung, kidney, muscle, skin, brain, cerebellum and hypophysis etc.
The jugular vein blood of step 12 blood sampling group collection utilizes liquaemin, ACD and EDTA-Na respectively
2anti-freezing, can repeatedly take a blood sample.
Step 10 nine reference family phenotype trait data storehouse comprises build group, suede grinds group, meat grinds group, breeding group and skin grind all proterties that group is collected, comprise: by hair color, the colour of skin, body body, angle-style, wattle, wrinkle, beard, nose, shank, ear type, hoof matter, style of the shoe, four limbs, udder shape, whether nipple is even, with or without additional teat, angular length (cm), ear length (cm), ear wide (cm), height (cm), body weight (kg), chest measurement (cm), circumference of cannon bone (cm), hip width (cm), testis encloses (cm), long (cm), the head breadth (cm), body long (cm), chest breadth (cm), chest depth (cm), hip cross high (cm), hair color, suede look, fine hair ratio, cashmere length (μm), cashmere diameter (μm), cashmere density (root/cm
2), pile layer height (cm), cashmere intensity (CN), clean suede rate, lambing type, lamb sex, parity, birth weight (g), cotyledon < 1cm, cotyledon>=2-3cm, cotyledon>=1-2cm, cotyledon>=4-5cm, cotyledon>=3-4cm, cotyledon>=5cm, Placental Weight (g), the wide footpath of cotyledon (cm), testis encloses (cm), sperm concentration, ejaculatory amount (μ L), sperm viability, rate of teratosperm, acrosomal integrity, sperm viability, sperm survival index, seminal fluid pH value, seminal fluid osmotic pressure, GOT(glutamic-oxalacetic transaminease) burst size, conception rate, birth weight (g), fatten starting weight (kg), weaning weight (kg), fatten whole weight (kg), 6 monthly ages heavy (kg), Slaughter weight (kg), age one full year of life heavy (kg), carcass weight (kg), 18 monthly ages heavy (kg), dressing percentage, grow up heavy (24 monthly age) (kg), trunk pure meat percentage, carcass quality, the thickness of backfat (cm), yellowish pink (5 points of systems), eye muscle area (cm
2), marbling (12 points of systems), plate thick (GR value), fat color (7 points of systems), meat bone ratio, percentage of water loss, pH value, storage loss rate, tenderness (shearing force), lean meat percentage, meat bone ratio, wadding of wool proterties, silk, wadding of wool size, staple length mensuration, flower case area, flower case definition, Wool density, hide thickness, identity area, plate matter, hair color, decorative pattern width, bending, pattern, gloss, the hair color uniformity, hide area, tare weight etc.
Claims (16)
1. a goat genetic resources base construction method, relates to the structure of the reference family of the national genetic resources Black goat of research and Cashmere Goat (Alxa type) proterties mechanism, it is characterized in that:
One, draft sources group constructing plan, formulate basic sign information record sheet, cashmere production record sheet, meat performance record sheet, reproductive performance record sheet, skin performance inventory table etc., prepare sample collection reagent consumptive material, experimenter divides into groups;
Two, require to buy consanguinity-less relation parental generation Black goat and Cashmere Goat (Alxa type) according to sources group constructing plan, Black goat ram (D) is used to carry out orthogonal with Cashmere Goat (I) ewe respectively, large black goat ewe (D) and Cashmere Goat ram (I) carry out reciprocal cross and obtain F1 generation, and positive and negative friendship is that offspring's male and female sheep avoids affiliation to carry out random mating obtaining F2 generation;
Three, build group membership when goat is born, 2 monthly ages (wean), 6 monthly ages, 12 monthly ages and 24 monthly ages carry out basic sign generaI investigation and record to all individualities of sources group respectively;
Four, suede grinds group and carries out grabbing suede season in goat lint every year, and all characteristic indexs measured involved by cashmere production placing on record;
Five, meat grinds group and measures when sources group goat is butchered and record the index that all meat performance record sheets relate to, and is responsible for gathering each tissue sample;
Six, breeding group is responsible for performing the sympathy relating to sources group and is oestrused technology, formulates breeding scheme and pedigree record, and gathers the listed reproductive trait parameter of reproductive performance record sheet;
Seven, skin grinds the responsible index gathered involved by all sources group individual skin performance inventory table of group;
Eight, blood sampling group gathers jugular vein blood (can multi collect) at 6 monthly ages, adopts liquaemin anti-freezing, ACD anti-freezing and EDTA-Na respectively
2anti-freezing, is numbered blood sample and registers related content;
Nine, test processed group I to be responsible for grinding to blood sampling group and meat the sample that group gathers and to carry out genomic DNA and organize sample RNA to extract, No. ID, check sample to preserve sample for subsequent use;
Ten, test processed group II to be responsible for excavating sources group individual SNP, SV and CNV variation data;
Ten, pedigree information database is set up: the breeding information relative recording according to breeding sets up sources group information database;
11, set up tissue samples storehouse: meat is ground the tissue samples that group gathers and load in aseptic cryopreservation tube, pipe shaft indicate No. ID, organization name, collection date etc., all tissue samples are placed in Liquid nitrogen storage according to a definite sequence, extract in order to DNA or RNA;
12, sources group DNA sample storehouse is set up: be stored in respectively after being checked digit by the sample genomic dna that experiment processed group is extracted (each individuality preserves 2 parts of genomic DNA samples) in-80 DEG C of ultra low temperature freezers;
13, sources group RNA Sample Storehouse is set up: be stored in liquid nitrogen for subsequent use after being checked digit by the RNA sample that experiment processed group is extracted;
14, genetic variation information database is set up: the variation data classifying type of experiment processed group II is deposited in ftp server;
15, sources group phenotype trait data storehouse is set up: comprise papery version database and electronics profile database;
2.papery version database is sealed up for safekeeping according to different classes of unification and is deposited; Electronics profile database, based on papery version record, has been formulated by Access software, and preservation of imprinting CDs;
3.and the record management work of database is responsible for by full-time researcher;
16, Black goat and Cashmere Goat (Alxa type) reference family total data storehouse (referred to as DIRF database) is set up: total data storehouse comprises pedigree information database, tissue sample storehouse, phenotype trait data storehouse, DNA database, RNA database and genetic variation information database, compilation total data library directory, formulation total data storehouse is checked and is received rule and management method.
2. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, it is characterized in that: step one sources group constructing plan relates to the positive and negative friendship of two kinds of national genetic resources Goats Breeds consanguinity-less relations, and positive and negative friendship offspring avoids affiliation random mating; Preparation comprises the formulation of breeding record sheet, basic sign information record sheet, cashmere production record sheet, meat performance record sheet, reproductive performance record sheet, skin performance inventory table; Experimenter is grouped into build group, suede grinds group, meat grinds group, breeding group, skin grind group, blood sampling group, experiment processed group I and II.
3. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, it is characterized in that: the proterties of step 3 build group collection comprises: by hair color, the colour of skin, body body, angle-style, wattle, wrinkle, beard, nose, shank, ear type, hoof matter, style of the shoe, four limbs, udder shape, whether nipple is even, with or without additional teat, angular length (cm), ear length (cm), ear wide (cm), height (cm), body weight (kg), chest measurement (cm), circumference of cannon bone (cm), hip width (cm), testis encloses (cm), long (cm), the head breadth (cm), body long (cm), chest breadth (cm), chest depth (cm), hip cross high (cm).
4. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, is characterized in that: step 4 suede grinds the proterties that group gathers and comprises: hair color, suede look, fine hair ratio, cashmere length (μm), cashmere diameter (μm), cashmere density (root/cm
2), pile layer height (cm), cashmere intensity (CN), clean suede rate.
5. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, is characterized in that: step 5 meat grinds the proterties that group gathers and comprises: birth weight (g), fatten starting weight (kg), weaning weight (kg), fatten whole weight (kg), 6 monthly ages heavy (kg), Slaughter weight (kg), age one full year of life heavy (kg), carcass weight (kg), 18 monthly ages heavy (kg), dressing percentage, grow up heavy (24 monthly age) (kg), trunk pure meat percentage, carcass quality, the thickness of backfat (cm), yellowish pink (5 points of systems), eye muscle area (cm
2), marbling (12 points of systems), plate thick (GR value), fat color (7 points of systems), meat bone ratio, percentage of water loss, pH value, storage loss rate, tenderness (shearing force), lean meat percentage, meat bone ratio.
6. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, it is characterized in that: the proterties that in step 6, breeding group is collected comprises: lambing type, lamb sex, parity, birth weight (g), cotyledon < 1cm, cotyledon >=2-3cm, cotyledon >=1-2cm, cotyledon >=4-5cm, cotyledon >=3-4cm, cotyledon >=5cm, Placental Weight (g), the wide footpath of cotyledon (cm), testis encloses (cm), sperm concentration, ejaculatory amount (μ L), sperm viability, rate of teratosperm, acrosomal integrity, sperm viability, sperm survival index, seminal fluid pH value, seminal fluid osmotic pressure, GOT(glutamic-oxalacetic transaminease) burst size, conception rate.
7. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, is characterized in that: step 7 skin grinds the proterties that group collects and comprises: wadding of wool proterties, silk, wadding of wool size, staple length measure, flower case area, flower case definition, Wool density, hide thickness, identity area, plate matter, hair color, decorative pattern width, bending, pattern, gloss, the hair color uniformity, hide area, tare weight.
8. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, it is characterized in that: step 8 blood sampling group gathers blood 5ml at every turn, at least gather three times, be placed in liquaemin anti-freezing, ACD anti-freezing and EDTA-Na respectively
2anti-freezing.
9. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, it is characterized in that: step 9 experiment processed group I takes phenol-chlorine extraction method to extract genomic DNA, adopt sky root kit to extract and organize RNA, then fill in DNA sample information table and RNA sample information table respectively.
10. Black goat and Cashmere Goat (Alxa type) reference family construction method according to claim 1, it is characterized in that: the experiment processed group II of step 10 utilizes genome sequencing and superchip screening mononucleotide pleomorphism (Singlenucleotidepolymorphism, SNP), structure variation (Structuralvariation, SV) and copy number variation (Copynumbervariation, the information such as CNV), these information according to different classes of and individual No. ID deposit in ftp server.
11. Black goat and Cashmere Goat (Alxa type) reference family construction methods according to claim 1, is characterized in that: step 9,12 organize sample and RNA sample be all stored in liquid nitrogen.
12. Black goat and Cashmere Goat (Alxa type) reference family construction methods according to claim 1, is characterized in that: the DNA sample of step 9 is stored in-80 DEG C of refrigerators.
13. Black goat and Cashmere Goat (Alxa type) reference family construction methods according to claim 1, is characterized in that: the phenotype trait data storehouse set up in step 15 comprises papery version archives, the electronics shelves Access database of typing and the cd resource of imprinting.
14. Black goat and Cashmere Goat (Alxa type) reference family construction methods according to claim 1, it is characterized in that: step 10 six DIRF reference family database comprises pedigree information database, tissue sample storehouse, phenotype trait data storehouse, DNA database, RNA database and variation information database, compilation total data library directory, formulation total data storehouse is checked and is received rule and management method.
15. 1 kinds of Black goats and Cashmere Goat (Alxa type) reference family construction method, it is characterized in that: construct Black goat and Cashmere Goat (Alxa type) reference family, construct Black goat and Cashmere Goat (Alxa type) genetic resource.
16. application in other Goat Population in Yangtse genetic resources of structure as method as described in any one in claim 1-15 or step.
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